JP3696941B2 - Method for preventing slime formation in industrial water systems - Google Patents

Description

【００２１】
次に、スライム形成能が認められるＡ、Ｂ及びＤに対して、共通的に殺菌効果を示す微生物撲滅剤を、常法にしたがって培養試験により、探索した結果、２，２‐ジブロモ‐２‐ニトロエタノールと２‐メチル‐５‐クロルイソチアゾリン‐３‐オンとを１：１で混合した製剤が有効であることが分った。
この微生物撲滅剤を、最大８０ｐｐｍの濃度で抄紙白水中に添加したところ、３０日間の操業中に、抄紙機中でのスライムの形成は認められなかった。
【００２２】
実施例２
紙パルプ製造工程の用水中にしばしば見出されるピンクコロニー形態を示す微生物は凝集物を形成しやすく、スライム生成の原因となっていることは当業者間に周知の事実である。
そこで、各社の抄紙白水中から、３種の異なったピンクコロニー形態を示す微生物（Ｆ、Ｇ、Ｈ）を採取し、実施例１で用いたのと同じセルロース繊維含有液体培地に接種し、実施例１と同じ条件下で培養して、増殖状況を顕微鏡で観察したところ、次の結果を得た。
【００２３】
（Ｆ） ピンクコロニー微生物Ｉ；セルロース繊維上で著しい菌体増殖及び凝集が認められた。また菌体の巨大な凝集物の中にセルロース繊維の塊が観察された。
（Ｇ） ピンクコロニー微生物ＩＩ；セルロース繊維上での菌体増殖が確認され、かつ菌体の凝集が認められた。
（Ｈ） ピンクコロニー微生物ＩＩＩ；セルロース繊維を抱き込んだ微生物の凝集物が観察された。
以上の結果から、紙パルプ製造工程におけるスライム形成の原因として知られているピンクコロニー形態を示す微生物は、いずれもセルロース繊維の表面において増殖することが分る。
【００２４】
次に、ピンクコロニー微生物Ｉについて、殺菌剤の効力試験を行い、２，２‐ジブロモ‐３‐ニトリロプロピオンアミドが有効であることを確認した。次いで、このピンクコロニー微生物Ｉを採取した抄紙白水中に同製剤を２０ｐｐｍの濃度で添加したところ、３０日経過後にわずかに淡黄色コロニーのスライムが認められたが、ピンクコロニーのスライムの発生は全く認められなかった。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for efficiently preventing slime formation of industrial wastewater discharged from various manufacturing industries and industrial water recycled after recycling treatment, such as white water in a papermaking process.
[0002]
[Prior art]
In industrial water systems that need to prevent slime formation by adding industrial disinfectants, such as white water in the papermaking process, there are many cases in which a plurality of microorganisms grow in the water system. However, single component fungicides are not sufficient and the synergistic effect of multiple component fungicides must usually be utilized.
[0003]
Until now, since the overall control has been carried out without specifying the type of microorganisms that grow in the water system, all microorganisms can be distinguished without distinction between microorganisms that form slime and microorganisms that are not related to slime formation. It was necessary to use a combination of ingredients capable of controlling.
[0004]
Therefore, for example, when trying to reduce troubles typified by the occurrence of slime during papermaking and the occurrence of spots on paper, it is common practice to use industrial disinfectants called slime control agents. However, since it is necessary to control all the microorganisms inhabiting in the system, it is necessary to use a combined preparation that combines multiple ingredients, and so far a combination of various active ingredients. Industrial disinfectants consisting of have been proposed.
[0005]
However, if the microorganisms that cause the generation of harmful slime can be detected in advance in the water system, a bactericide containing only components for controlling the microorganisms may be used, and unnecessary components may be omitted. Since it can be done, it should be very advantageous in terms of cost.
[0006]
[Problems to be solved by the invention]
The present invention accurately grasps microorganisms that cause slime formation in industrial water systems, and uses only an effective bactericide for the microorganisms to efficiently prevent slimes in industrial water systems. It was made for the purpose of providing.
[0007]
[Means for Solving the Problems]
As a result of extensive research on the method for preventing slime formation in industrial water systems, the present inventor has a majority of slime in the paper making process aggregated by microorganisms inhabiting white water to form flocs. Noting that based on the phenomenon, by agglomerating cellulose fibers was observed whether slime formation is done by observing the behavior of individual microorganisms in a liquid medium containing cellulose fibers, with respect to microbial slime formation is made Thus , it has been found that if only an effective bactericide is introduced, slime formation in white water can be efficiently prevented, and the present invention has been made based on this finding.
[0008]
That is, the present invention cultivates and proliferates a microorganism sample collected from an industrial water system , then isolates each microorganism present therein, and each of the isolated microorganisms is a liquid medium containing cellulose fibers individually. They were cultured in the medium, and observing the presence or absence of formation of aggregates bound to cellulose fibers, select an effective disinfectant with the microorganisms to form aggregates, and Turkey be added only the sterilizing agent to the industrial water An industrial water-based slime formation preventing method is provided.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Industrial water systems to be treated according to the present invention include, in addition to white water in the papermaking process described above, food manufacturing industry, chemical seasoning manufacturing industry, sugar refinery industry, brewing industry, rayon industry, papermaking, silk smelting industry, wool industry And waste water and reclaimed water in the oil and fat industry, pharmaceutical manufacturing industry, petroleum industry, leather manufacturing industry and the like.
[0010]
Next, the culture and growth of microbial samples collected from these industrial water systems are carried out using known media commonly used for culturing various microorganisms, such as ordinary agar, asparagine / glucose agar, bouillon agar, zapec It can be carried out by inoculating microorganisms and keeping the culture conditions in accordance with a conventional method using a solid agar medium, starch agar medium, egg agar medium, Waxman agar medium, gelatin agar medium and the like.
[0011]
After incubation, the colonies of the samples grown on medium, optionally using a microscope, isolated City each microorganisms present therein, a liquid medium containing the isolated each individual cellulose fibers microorganisms Inoculate. The liquid medium in this case is not particularly limited, and L-medium, LB-medium, meat extract medium, tryptosy bouillon medium, YCC liquid medium, etc. are used. Waxman liquid medium is used in terms of the transparency of the medium. Is preferred. Moreover, as a cellulose fiber included in this liquid culture medium, the various cellulose fiber which exists in the industrial water system which produces pulp fiber and slime formation is used. This cellulose fiber preferably has a diameter of 5 to 50 μm and a length of 2 to 10 mm.
[0012]
In the method of the present invention, it is observed using a microscope as necessary whether or not aggregates bound with fiber cellulose are formed in the culture medium after culturing . At this time, it is advantageous to stain the microorganisms in accordance with a conventional method, but it can also be observed as it is using a phase contrast microscope without staining.
[0013]
As described above, it binds to cellulose fibers to form aggregates , searches for microorganisms that cause slime formation, and if specified, selects a bactericidal agent effective against these microorganisms. Only bactericides are added to industrial water systems from which microbial samples have been collected. The bactericides used at this time include those commonly called antibacterial and antifungal agents and those called bacteriostatic agents.
[0014]
As such a bactericidal agent, those effective against the target microorganism are appropriately selected and used from among phenolic drugs, thiazole drugs, isothiazole drugs, bromine drugs, iodine drugs, and the like. This disinfectant may be used alone or in combination of two or more. These are added to the water system at a concentration in the range of 1 to 100 ppm in the case of the paper industry, and in the range of 20 to 400 ppm in the case of the oil and fat industry, leather industry, food industry and the like.
[0015]
【The invention's effect】
According to the present invention, by using a liquid medium to which cellulose fiber is added, it is possible to easily reproduce the occurrence of slime in a test tube and to identify which microorganisms are involved in slime formation. Therefore, an effective disinfectant can be selected for microorganisms involved in slime formation, and by adding only this disinfectant , the required industrial water-based slime can be efficiently formed.
[0016]
【Example】
Next, the present invention will be described in more detail with reference to examples.
[0017]
Example 1
The slime adhering to the paper machine in operation at Company A was collected, inoculated into Waxman agar medium prepared in Petri, and cultured at 30 ° C. for 24 hours. The colonies were observed with a microscope. It turned out that it is comprised from five types and the number of bacteria of (A)-(E) shown.
[0018]
Next, according to a conventional method, these 5 bacterial species were completely isolated, transferred to a test tube containing Waxmann liquid medium to which 1% by weight of pulp fiber was added, and cultured with shaking at 33 ° C. for 24 hours. The resulting culture is thoroughly agitated and then treated to a final concentration of 0.05 M Tris-HCl buffer (pH 9.5) and 5% sodium chloride solution to obtain carboxyfluorescein diacetate (5-, 6-mixture). 10 ppm and propidium iodide 50 ppm were added and dyed.
Each sample thus prepared was observed with a B excitation wavelength using a fluorescence microscope, and the following results were obtained.
[0019]
(A) Pale yellow sticky bacterium: Cell growth on cellulose fibers was performed, and it was confirmed that the grown cells aggregated cellulose fibers and caused slime.
(B) Yellow slimy bacterium: It was confirmed that remarkable bacterial cell growth was carried out on the cellulose fiber, causing aggregation. Moreover, the lump of the cellulose fiber was observed in the giant aggregate of the microbial cell.
(C) Yellow slime bacterium; no bacterial growth on cellulose fibers was observed. It can therefore be seen that this does not cause slime formation.
(D) Pale brown mucous bacteria; marked cell growth and aggregation on cellulose fiber was confirmed. Moreover, the lump of the cellulose fiber was observed in the huge aggregate of the microbial cell.
(E) Light brown mucous bacterium; no bacterial growth on cellulose fibers was observed. Therefore, it was found that this bacterium was not responsible for slime formation.
From the above results, bacteria causing slime formation were confirmed, and the results are shown in Table 1.
[0020]
[Table 1]

[0021]
Next, as a result of searching for a microorganism eradicating agent having a common bactericidal effect on A, B and D with recognized slime forming ability by a culture test according to a conventional method, 2,2-dibromo-2- It has been found that a 1: 1 mixture of nitroethanol and 2-methyl-5-chloroisothiazolin-3-one is effective.
When this microbe eradication agent was added to papermaking white water at a maximum concentration of 80 ppm, no slime formation was observed in the papermaking machine during 30 days of operation.
[0022]
Example 2
It is a well-known fact among those skilled in the art that microorganisms exhibiting pink colony morphology often found in the water used in the pulp and paper manufacturing process tend to form aggregates and cause slime formation.
Therefore, three types of microorganisms (F, G, H) showing different types of pink colonies were collected from the papermaking white water of each company, and inoculated into the same cellulose fiber-containing liquid medium as used in Example 1. When the cells were cultured under the same conditions as in Example 1 and the growth state was observed with a microscope, the following results were obtained.
[0023]
(F) Pink colony microorganism I: Remarkable cell growth and aggregation were observed on cellulose fibers. Cellulose fiber clumps were observed in the huge aggregates of the cells.
(G) Pink colony microorganism II; microbial cell growth on cellulose fibers was confirmed, and microbial cell aggregation was observed.
(H) Pink colony microorganism III: Aggregates of microorganisms embracing cellulose fibers were observed.
From the above results, it can be seen that all the microorganisms showing the pink colony form known as the cause of slime formation in the paper pulp manufacturing process grow on the surface of the cellulose fiber.
[0024]
Next, the efficacy test of the bactericide was performed on the pink colony microorganism I, and it was confirmed that 2,2-dibromo-3-nitrilopropionamide was effective. Subsequently, when the same formulation was added at a concentration of 20 ppm to the papermaking white water from which the pink colony microorganism I was collected, a slight pale-colony colony slime was observed after 30 days, but the occurrence of slime in the pink colony was completely absent. I was not able to admit.

Claims (2)

After grown by culturing a microorganism samples taken from industrial water, isolated each microorganisms present therein, culturing each of the isolated microorganisms in a liquid medium containing individually cellulose fibers, cellulose observing the presence or absence of formation of aggregates bound to the fibers, with a microorganism to form aggregates select an effective disinfectant, industrial water system characterized by adding only the sterilizing agent to the industrial water Slime formation prevention method. Slime formation prevention process according to claim 1, wherein the industrial water system is a manufacturing paper white water.