JP3694806B2 - Multi-component quantitative method and multi-component quantitative device - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention is a multi-component quantification method for quantifying at least two components contained in a substance to be measured by color chemistry measurement and a multi-component quantification apparatus using the method, and in particular, component quantification from coordinates in a color system coordinate space. The present invention relates to a multi-component quantification method for acquiring data for use and a multi-component quantification apparatus using the method.
[0002]
[Prior art]
Concentration is generally an indispensable operation when quantifying trace metal elements present in environmental samples. This is because a large amount of various organic and inorganic substances are present in environmental samples, but the amount of substances involved in the measurement is very small, such as on the order of μg or ng or less. Because there is. In recent years, the performance of analyzers has improved rapidly, and the detection limit value has decreased, but in order to fully exploit the performance of analyzers, pre-separation and pre-concentration with little trace element contamination and loss are increasing. It is becoming important. In addition, instrumental analyzers such as atomic absorption, emission spectrometry, and various types of chromatography are more complex and expensive as the sensitivity is higher. Therefore, the installation is expensive and the operation is not easy. In particular, in use, running costs such as carrier gas, combustion gas, and cooling water are also required, and preparation thereof is troublesome.
[0003]
In addition, as described in Japanese Patent Application Laid-Open No. 10-73533, as a solid phase color substance quantification method including coexisting color substances, a sample is irradiated with light, and the chromaticity coordinates and light quantity of the reflected light are determined by three. Reflection from the sample based on the measured chromaticity coordinates, the measured chromaticity coordinates, the chromaticity coordinates of the coexisting colored objects, and the chromaticity coordinates of the light output from the light source. There is a method of calculating the amount of the light component and determining the content of the colored object based on the calculated amount of light.
[0004]
[Problems to be solved by the invention]
As described above, analyzers such as atomic absorption, emission spectrometry, and various chromatographs are expensive to install, and are not easy in terms of handling. Yes. The technique described in Japanese Patent Application Laid-Open No. 10-73533 is used as a solid-phase color quantification method including a coexisting color product, but basically removes the reflected light component of the co-existing color product. By doing so, only the sample is quantified, and two or more components cannot be quantified simultaneously.
[0005]
Therefore, in view of the above technical problems, the present invention provides a multi-component quantification method capable of simultaneously quantifying two or more components while maintaining the simplicity of measurement, and a multi-component quantification apparatus using the method. The purpose is to provide.
[0006]
[Means for Solving the Problems]
  In the multi-component quantification method of the present invention, a multi-component calibration surface formed by intersecting a plurality of calibration curves is used for at least two components contained in the measurement target substance.pluralAfter forming in the color system coordinate space, obtain coordinates in the color system coordinate space of the measurement target substance from the color difference information of the measurement target substance,Select a color system coordinate space in which the mesh formed by the intersection of the calibration curves on the multi-component calibration surface from the plurality of color system coordinate spaces exhibits a calibration surface that is wider than other meshes,Extending the coordinates of the measurement target substance in the color system coordinate space from the position of the multi-component calibration plane along each calibration curve to obtain a component value corresponding to the measurement target substance and quantifying each component Features.
[0007]
When at least two components are expressed in the color system coordinate space, a multi-component calibration surface formed by intersecting calibration curves for each component is obtained. Therefore, if the color difference information of the substance to be measured can be obtained, the position on the multi-component calibration surface can be known, and each component can be quantified by extending from the position along each calibration curve. At this time, since the multi-component calibration surface has a shape reflecting the tendency of the curve in the color system coordinate space of each calibration curve, more accurate quantification is realized.
[0008]
  Further, the multi-component quantification device of the present invention is a measuring means for reading color difference information from light irradiation on the measurement target substance,Corresponding to a plurality of color system coordinate spaces,Calibration surface data storage means for storing multicomponent calibration surface data of at least two components contained in the substance to be measured;A multi-component calibration corresponding to a color system coordinate space in which a mesh formed by an intersection of calibration curves on the multi-component calibration surface from among the plurality of color system coordinate spaces exhibits a calibration surface that is wider than other meshes. After selecting the face dataCalculation means for obtaining the position of the multi-component calibration surface based on the color / color difference information, and quantification means for obtaining a component value corresponding to the substance to be measured from the position of the multi-component calibration surface and quantifying each component. It is characterized by.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
In the multi-component quantification method of the present invention, the multi-component calibration surface formed by intersecting a plurality of calibration curves is formed in the color system coordinate space for at least two components contained in the measurement target material, and then the measurement target material The coordinate in the color system coordinate space of the measurement target substance is obtained from the color difference information of the measurement object, and the coordinates in the color system coordinate space of the measurement target substance are extended from the position of the multi-component calibration plane along each calibration curve. Then, a component value corresponding to the measurement target substance is obtained, and each component is quantified.
[0010]
The multi-component quantification method of the present invention is a method for quantifying two or more components at the same time in principle, and combinations of the components include chemical or physical shading change depending on the concentration, or It is an organic compound or an inorganic compound in which a hue change, a saturation change, a brightness change, etc. occur, and various components such as an inorganic compound such as a metal ion, an organic compound such as a dye, and a complex thereof can be measured.
[0011]
For example, in the case of quantifying two components, when a single equilibrium is established between the two components of interest and the colors of the two components change, a method of measuring the substance or condition governing the equilibrium by color measurement As an example, the multi-component quantification method of the present invention can be applied to a pH measurement method using a color change due to acid dissociation equilibrium of a dye.
[0012]
More specifically, the multicomponent quantification method of the present invention can be used for each of the following substances. That is, (1) the multi-component quantification method of the present invention can be applied to the measurement of useful or harmful components contained in river water, ground water, tap water, rain water, sea water, lake water, hot spring water, drinking water, pure water and the like. For example, these waters may contain mercury, chromium, arsenic, lead, residual chlorine, etc., and even when one element is dissolved in the form of a plurality of metal ions, it can be quantified. (2) The multi-component quantification method of the present invention can be applied to gas concentration tests in the atmosphere, gas products, and exhaled breath. Since the multi-component quantification method of the present invention uses direct color difference information, it is easy to measure a substance phase in a gas state regardless of the solid phase, liquid phase, or gas phase. is there. For example, two components or more of various gases such as NOx, SOx, hydrogen sulfide, carbon monoxide, oxygen concentration, alcohol and aldehyde can be measured simultaneously.
[0013]
The multi-component quantification method of the present invention further comprises (3) measurement of trace components in living body, diagnostic agent (trace substances contained in blood, plasma, urine, etc.), (4) measurement of soil and fertilizer content in soil. (Nitrogen, phosphorus, potash, etc.), (5) surface temperature measurement (liquid crystal thermometer, etc.), (6) humidity, moisture measurement, (7) food color and freshness management (fish, fruit, fruit juice, vegetables, meat, Cereals, etc.), (8) Growth status and product confirmation in agriculture and forestry (flowers, leaves, fruits, wood, etc.), (9) Quality control of colored products (fabrics, clothing, automobiles, furniture, building materials, etc.) , Medicines such as tablets, foods such as cosmetics, confectionery and beverages, electronic equipment, household appliances, printed materials, polymers, etc.), (10) determination of deteriorated products, deteriorated buildings (concrete, rubber, metal, etc.), (11) Quality control of products whose surface condition is controlled by reflected light (half Body, glass, etc.), (12) color diagnosis of skin, eyes, etc. (jaundice, sunburn degree, skin whiteness, personal identification by eyeball, etc.), (13) redox atmosphere test (iron (II) and iron (III) (14) can be used as a quantitative method for simultaneously quantifying multiple components in various fields such as quantification of quantitative values in simple test methods such as pH test paper, ion test paper, and pack test. .
[0014]
Next, with respect to each step of the multicomponent quantification method of the present invention, referring to the flowchart of FIG. 1 and the schematic diagram of the L * a * b * (a * b *) color coordinate space in FIG. explain.
[0015]
As shown in FIG. 1, first, a multi-component calibration surface is formed in the color system coordinate space (step S11). In this first stage, it is assumed that a substance to be measured is selected, and two or more components related to measurement are also selected. For example, when the measurement medium is a solution such as ground water and the metal to be quantified is Co, Ni, Fe, etc., a calibration curve corresponding to each component is first drawn in the color system coordinate space, and each intersection point A mesh-like (mesh-like) calibration surface is formed by a method for obtaining the above. Here, for convenience, two components in the substance to be measured are referred to as a first component and a second component.
[0016]
FIG. 2 shows an example in which a multi-component calibration surface (in this case, two components) is formed on the a * b * plane in the L * a * b * color system which is such a color system coordinate space. . In FIG. 2, the curve indicated by the black circle is the calibration curve K1 for the first component, and the curve indicated by the black square is the calibration curve K2 for the second component. The calibration curve K1 of the first component is given number 1, number 2, number 3, and number 4 from the side close to the origin, and each number corresponds to a predetermined concentration or the number of micrograms contained (μg). Yes. That is, for example, each black dot of No. 1, No. 2, No. 3, and No. 4 corresponds to the number of contained micrograms of 0.1 μg, 0.2 μg, 0.3 μg, and 0.4 μg. Similarly, the calibration curve K2 of the second component is given number 1, number 2, number 3, and number 4 from the side close to the origin, and each number has a predetermined concentration or the number of micrograms contained (μg). Correspond. That is, for example, each black square of No. 1, No. 2, No. 3, and No. 4 corresponds to the number of micrograms contained in 0.1 μg, 0.2 μg, 0.3 μg, and 0.4 μg.
[0017]
The calibration curve K1 for the first component and the calibration curve K2 for the second component are formed so as to intersect at an angle with the origin as the center, and constitute a mesh-like (mesh-like) two-component calibration surface. More calibration points are formed in FIG. For example, in FIG.₂₂Is configured by plotting the coordinates of the color system measured with the microgram content of the first component being 0.2 μg and simultaneously the microgram content of the second component being 0.2 μg. Similarly, the calibration point P₄₃Is configured by plotting the coordinates of the color system measured with the number of micrograms contained in the first component being 0.4 μg and simultaneously the number of micrograms contained in the second component being 0.3 μg. With regard to the calibration points on such a two-component calibration surface, it is possible to construct a two-component calibration surface having a sufficient mesh shape (mesh) by measuring all the calibration points. It is also possible to obtain the missing points by calculation.
[0018]
As shown in FIG. 2, each calibration curve constituting the two-component calibration surface is not a straight line, and the intervals between calibration points are not even intervals. For this reason, the calibration method based on the simple proportional distribution method or proportional distribution method increases the error, but the calibration error can be minimized by extending along the curve as will be described later. Each calibration curve is obtained by such measurement, and in the multicomponent quantification method of the present invention, a multicomponent calibration surface can be formed. In FIG. 2, a multi-component calibration surface is formed by obtaining coordinates on the a * b * plane in the L * a * b * color system. However, depending on the sample, in the L * a * b * color system An L * a * plane or an L * b * plane can be used, and an xy plane, an xY plane, or a yY plane of an XYZ color system can also be used. Furthermore, other color space coordinates such as the L * u * v * color system, the UCS color system, and the Hunter Lab color system can also be used.
[0019]
As a method for forming a multi-component calibration surface, the multi-component calibration surface can be obtained by actually measuring the components, but in addition to that, two-component calibration of A component and B component in advance. Surface data can be acquired and used for measurement at different times and locations depending on storage, storage, transfer, etc.
[0020]
Next, according to the flowchart of FIG. 1, when the multi-component calibration surface is formed, the coordinates of the substance to be measured are measured with a color difference meter (step S12). A color difference meter is a measuring device for measuring coordinate values of a color system, such as CR-300, CR-310, CR-331, CR-331C, CR-321, CR-241, manufactured by Minolta Co., Ltd. Measuring devices such as CT-310, CT-320, CS-100, CL-100, SQ2000, SE2000, ZE2000, VSS300H, NF777, NF999, NR-3000A / B, NDR-2000 manufactured by Nippon Denshoku Industries Co., Ltd. ND-1001DP, NR-1, BP-1, etc. can be used, and other color-difference meters can be used as long as the color-difference meter can coordinate the measurement target substance with required accuracy. A color difference meter may be used.
[0021]
In this measurement, the substance to be measured may be a solid phase, or a liquid phase or a gas phase. When moisture is mixed in the measurement target substance, the measurement may be performed after drying. By measuring with a color difference meter, coordinate data of the color system space of the substance to be measured is output. The data format can be selected depending on the color difference meter, and the data format is selected according to the aforementioned multi-component calibration surface. As an example, the coordinates on the a * b * plane in the L * a * b * color system are output, but the coordinates of the L * a * plane in the L * a * b * color system or L * b * Plane coordinates may be used. Furthermore, it is also possible to use coordinates such as the xy plane, the xY plane, or the yY plane of the XYZ color system. Furthermore, other color space coordinates such as the L * u * v * color system, the UCS color system, and the Hunter Lab color system can also be used.
[0022]
When the coordinate data of the color system space of the measurement target substance is obtained, the position of the coordinates of the color system coordinate space obtained by measurement is obtained on the multi-component calibration surface as shown in step S13 of FIG. For example, in FIG. 2, the obtained coordinate data is (a *₁, b *₁), The obtained coordinate data (a *₁, b *₁) Is a coordinate point on the a * b * plane and occupies a position indicated by a cross-shaped point. The coordinate data obtained at this time (a *₁, b *₁) Is not necessarily on the line of each calibration curve, and may be coordinate data that takes a position inside the mesh-like mesh.
[0023]
Next, as shown in step S14 of FIG. 1, the component amounts are obtained simultaneously by extending the coordinates of the measured color system coordinate space along the calibration curve. This process will be described with reference to FIG. 2. The coordinate data (a *) obtained by measuring the measurement target substance.₁, b *₁), Extension lines E1 and E2 are drawn as indicated by dotted lines in the figure. The extension line E1 is a line extended so as to intersect the calibration curve K1 of the first component, and the extension method is coordinate data (a *₁, b *₁) Is maintained in accordance with the distance between the pair of calibration curves sandwiching), and the same is extended in the next mesh as it is. Here, the slope according to the distance is close to one of the calibration curves, the slope of the extension line is close to the slope of the calibration curve on the near side. For example, in the case of just the midpoint, a pair of calibration curves Take the average slope. The extension line E2 is a line extended so as to intersect the calibration curve K2 of the second component, and the extension method is the same as the extension line E1, and the coordinate data (a *₁, b *₁) Is maintained in accordance with the distance between the pair of calibration curves sandwiching), and the same is extended in the next mesh as it is.
[0024]
In the multicomponent quantification method of the present invention, the amount of each component can be obtained simultaneously by extending along such a calibration curve. That is, the intersection of the extension line E1 and the first component calibration curve K1 is the first component quantification point, and the intersection of the extension line E2 and the second component calibration curve K2 is the second component quantification point. In the case of FIG. 2, for example, the first component can be calculated as 0.14 μg, and the second component can be calculated as 1.15 μg.
[0025]
Next, the creation of a multi-component calibration surface will be described in further detail. First, methylene blue (basic blue 9), which is a blue pigment, three dyes, safranin T (basic red 2), which is a red pigment, and thioflavine, which is a yellow pigment, are used to create a three-component calibration surface. Using three types of cationic dyes (Basic Yellow 1), mixing at various ratios and amounts, 0.5 ml of a cation exchange resin suspension (Dowex HCR-S 8.4 μequiv. / Ml) is added. Next, 1 ml of acetic acid-sodium acetate buffer solution (pH 5) is added to make the total volume 20 ml, and suction filtration is performed with a membrane filter (0.65 μm). After drying the obtained solid phase, coordinate data in the color system is obtained by a color difference meter to form a multi-component calibration surface. For quantification of two components (for example, two components of methylene blue and safranin T), the two-component portion of the multi-component calibration surface formed with such three components may be used.
[0026]
FIG. 3 is a diagram showing an example of a calibration surface obtained by such a method, and the coordinates on the a * b * plane in the L * a * b * color system are obtained. Further, the multi-component calibration surface shown in FIG. 4 is formed from the coordinates on the xy plane in the XYZ color system using the same three types of cationic dyes. FIG. 5 shows the coordinates on the L * a * plane in the L * a * b * color system, and FIG. 6 shows the coordinates on the Yx plane in the XYZ color system. Further, FIG. 7 shows the coordinates on the L * b * plane in the L * a * b * color system, and FIG. 8 shows the coordinates on the Yy plane in the XYZ color system.
[0027]
As shown in FIGS. 3 to 8, the calibration surface shows different calibration curves and calibration surfaces even for the same component depending on the coordinate axis and the color system. Therefore, when the calibration curves overlap each other too much, even if the color coordinate system coordinate data is measured with a color difference meter with respect to the object to be measured, quantification based on the data becomes difficult, so it is formed by the intersection of the calibration curves. It is preferable to select a calibration surface having a structure in which the mesh itself is greatly expanded. In particular, the three-component calibration surface will be explained. When the coordinates of the L * a * b * color system are exchanged, a cube with a slightly distorted mesh structure is displayed to rotate, and there is no other pigment component. The lines constitute the edges of the cube with its slightly distorted mesh structure.
[0028]
Similarly, FIG. 9 and FIG. 10 show that three dyes, methylene blue (basic blue 9), a red dye, safranin T (basic red 2), and three dyes are used to create a three-component calibration surface. In this example, three types of cationic dyes, thioflavine (basic yellow 1), which is a yellow dye, were measured in a liquid phase with a total volume of 25 ml. NDR- manufactured by Nippon Denshoku Industries Co., Ltd. was used for the measurement. Measurement is performed using 2000.
[0029]
FIG. 9 shows the coordinates on the a * b * plane in the L * a * b * color system. Further, the multi-component calibration surface shown in FIG. 10 is formed from coordinates on the xy plane in the XYZ color system using the same three types of cationic dyes. As is clear from FIGS. 9 and 10, coordinate data can be obtained in the same manner as the solid phase for the liquid phase sample, and multi-component simultaneous calibration is realized.
[0030]
Next, a measurement example of the metal concentration that forms a colored complex will be described. FIG. 11 shows calibration curves for mercury (Hg), iron (Fe (II) and Fe (III)), cobalt Co (II) and Co (III), and nickel (Ni (II)). Fe (II) is calibrated in two types: Nitroso-PSAP complex (PSAP = 2-nitroso-5- (N-propyl-N-sulfopropylamino) phenol) and BPS complex (BPS = Bathophenanthroline disulfonic acid). .
[0031]
In FIG. 11, only the portion of the calibration curve before the calibration surface is formed is drawn, and the multi-component calibration surface may be configured by selecting two components, that is, two calibration curves. FIG. 11 shows the coordinates on the a * b * plane in the L * a * b * color system. The L * a * plane or the L * b * plane in the L * a * b * color system. Furthermore, it is also possible to use an xy plane, an xY plane, or a yY plane of the XYZ color system. Furthermore, other color space coordinates such as the L * u * v * color system, the UCS color system, and the Hunter Lab color system can also be used.
[0032]
Regarding the preparation of calibration curves for cobalt and nickel, first, 1 × 10^-3After adding 1 ml of Nitroso-PSAP solution of M, the pH is adjusted to weak acidity and stirred. Next, 0.5 ml of anion exchange resin suspension (Diaion, manufactured by Mitsubishi Chemical, PA316, 16 μequiv. / Ml) is added and stirred, followed by suction filtration with a membrane filter (0.65 μm). Then, the obtained solid phase is measured with a color difference meter in a wet state to obtain a Ni (II) -Nitroso-PSAP curve and a Co (II) -Nitroso-PSAP curve in FIG. These two Ni (II) -Nitroso-PSAP and Co (II) -Nitroso-PSAP curves are both extended in the first quadrant of the a * b * plane and are not overlapping. It is easy to construct a multi-component calibration surface from these two curves, and a multi-component calibration surface having a mesh structure can be easily created.
[0033]
In FIG. 11, each curve of Hg-STTA, Fe (II) -BPS, Fe (II) -nitroso-PSAP, Fe (III) -nitroso-PSAP, Co (III) -5-Br-PSAA ( A multi-component calibration surface of mesh structure with any two curves out of a total of seven curves of Ni (II) -Nitroso-PSAP and Co (II) -Nitroso-PSAP) It can be created.
[0034]
STTA has the chemical structure shown in FIG. 12, and is an abbreviation for 1,1,1-Trifluoro-4-mercapto-4- (2-thienyl) -3-butene-2-one. Further, 5-Br-PSAA has the chemical structure shown in FIG. 13 and includes 2- (5-Bromo-2-pyridylazo) -5- [Nn-propyl-N- (3-sulfopropyl) amino] aniline, Abbreviation for sodium salt. The structural formula of Nitroso-PSAP is shown in FIG. These complex constituent agents are examples, and other complex constituent agents can be used as the multicomponent quantitative method of the present invention.
[0035]
Next, an embodiment of the multi-component quantitative apparatus of the present invention will be described with reference to FIG.
[0036]
The multi-component quantification apparatus according to the present embodiment includes a measurement unit that reads color difference information from light irradiation on a measurement target substance, and a calibration plane data storage that stores data of a multi-component calibration plane of at least two components included in the measurement target substance. Means for calculating the position of the multi-component calibration surface based on the color difference information; and a quantification unit for determining the component value corresponding to the measurement target substance from the position of the multi-component calibration surface and quantifying each component; It is characterized by having.
[0037]
FIG. 15 is a block diagram showing the configuration of the multi-component quantitative apparatus. A light source 11 such as a xenon lamp is provided so as to face the measurement target substance 10, and the light source 11 is controlled to be turned on or off by power supply from the illumination circuit unit 12. A hole is provided in the central portion of the light source 11, and an optical system 13 including a fiber lens or the like is installed so as to collect the reflected light on the surface of the measurement target substance 10. The light thus collected is collected in the light receiving unit 14 and output from the photoelectric conversion in the form of an electrical signal. Since the light receiving unit 14 is formed with a light receiving element such as a CCD image pickup element and receives light through a required filter, this electrical signal becomes a signal representing the coordinates of the color system reflecting the tristimulus values.
[0038]
Further, in the multi-component quantitative apparatus of the present embodiment, a control unit 15 is formed through an I / O unit 17 that receives an electrical signal from the light receiving unit 14. The control unit 15 is a part that mainly calculates the coordinate data of the multi-component calibration surface. For example, at the stage of creating the multi-component calibration surface, a plurality of samples are placed under the light source 11 and measured. Therefore, coordinate data corresponding to each sample is written in the storage unit 20 in the form of a table. The control unit 15 is capable of key input and is connected to the keypad unit 18. The monitor 16 is also connected to the control unit 15, and information regarding the multi-component calibration surface accumulated in the storage unit 20 and information such as the state of the apparatus can be displayed on the screen of the monitor 16. Further, it is possible to print out as required, and required printing can be performed from the printer 19 connected to the control unit 15.
[0039]
  In the control unit 15, a calculation process for creating a multi-component calibration surface, and any part of the multi-component calibration surface when the measurement target substance 10 is actually measuredInArithmetic processing for determining whether it is located and arithmetic processing for quantifying each component from the position on the multi-component calibration surface are performed.In particular, the calculation processing for creating a multi-component calibration surface can be made to correspond to a plurality of color system coordinate spaces, and a calibration curve on the multi-component calibration surface can be selected from the plurality of color system coordinate spaces. It is possible to select multi-component calibration surface data corresponding to the color system coordinate space in which the mesh formed by the intersection of the two colors exhibits a calibration surface that is wider than the other meshes.
[0040]
The multi-component quantification apparatus having such a configuration can measure the measurement target substance 10 and simultaneously specify a plurality of component amounts contained therein. For this purpose, a multi-component calibration surface for comparison is held in the form of data. The multi-component quantification apparatus of this embodiment stores a method for preparing and reading a plurality of samples for creating a multi-component calibration surface immediately before measuring a measurement target substance and past data. Selecting a method of storing in the unit 20 and reading the past data and processing, and a method of distributing data relating to the multi-component calibration surface as standard data in the form of the memory card 22 or the like Can do. In particular, when the standard data of the memory card 22 is stored and the apparatus is operated, the measurement from the measurement target substance 10 becomes possible simply by inserting the memory card 22 into the insertion portion 21 of the memory card 22. Therefore, even when uniform quality control is required at multiple factories, uniform quality control is realized by storing and distributing the data related to the standardized multi-component calibration surface in the memory card 22. Data changes can be made smoothly because digital data changes.
[0041]
  According to the multi-component quantification method and multi-component quantification apparatus of the present invention, it is possible to quantitate two or more components at the same time while maintaining the simplicity of measurement. Since the coordinate value is used for quantification of each component, the accuracy of the quantification work can be sufficiently increased.In particular, according to the multi-component quantification method and multi-component quantification apparatus of the present invention, the mesh formed by the intersection of the calibration curves on the multi-component calibration surface from the plurality of color coordinate space is more than other meshes. Since a plurality of components are quantified after selecting a color system coordinate space that exhibits a wide calibration surface, reliable and highly accurate quantification is possible even when certain calibration curves overlap each other. Done.
[0042]
In the measurement, a color difference meter or a multi-component quantification apparatus as described above can be used, and in that case, prompt quality measurement and prompt on-site response can be promoted. In addition, a color difference meter can be miniaturized in general, so it greatly contributes to on-site analysis.
[Brief description of the drawings]
FIG. 1 is a flowchart of a multi-component quantitative method according to an embodiment of the present invention.
FIG. 2 is an L * a * b * color system space diagram showing a multi-component quantitative surface in the multi-component quantitative method of one embodiment of the present invention.
FIG. 3 is an L * a * b * color system space diagram showing a quantitative surface of a pigment-based multicomponent, and is a diagram plotted on an a * b * plane.
FIG. 4 is an XYZ color system space diagram showing a quantitative surface of a pigment-based multicomponent, plotted on the xy plane.
FIG. 5 is an L * a * b * color system space diagram showing a quantitative surface of a dye-based multicomponent, and is a diagram plotted on an L * a * plane.
FIG. 6 is an XYZ color system space diagram showing a quantitative surface of a dye-based multicomponent, plotted on the Yx plane.
FIG. 7 is an L * a * b * color system space diagram showing the quantitative surface of a dye-based multicomponent, and is a diagram plotted on the L * b * plane.
FIG. 8 is an XYZ color system space diagram showing a quantitative surface of a dye-based multicomponent, plotted on the Yy plane.
FIG. 9 is an L * a * b * color system space diagram showing a quantitative surface of a liquid phase dye-based multicomponent, and is a diagram plotted on an a * b * plane.
FIG. 10 is an XYZ color system space diagram showing a quantitative surface of a liquid phase dye-based multicomponent, and is a diagram plotted on an xy plane.
FIG. 11 is an L * a * b * color system space diagram showing a calibration curve for each metal that forms a colored complex, plotted on the a * b * plane.
FIG. 12 is a chemical structure diagram showing the structure of STTA.
FIG. 13 is a chemical structural diagram showing the structure of 5-Br-PSAA.
FIG. 14 is a chemical structure diagram showing the structure of nitroso-PSAP.
FIG. 15 is a block diagram showing an embodiment of the multi-component quantitative apparatus of the present invention.
[Explanation of symbols]
10 Substances to be measured
11 Light source
12 Lighting circuit
13 Optical system
14 Receiver
15 Control unit
16 Monitor
17 I / O section
18 Keypad part
19 Printer
20 storage unit
21 Insertion section
22 Memory card

Claims (6)

A multi-component calibration surface formed by intersecting a plurality of calibration curves is formed in a plurality of color system coordinate spaces for at least two components included in the measurement target material, and the measurement is performed from the color difference information of the measurement target material. Calibration in which the coordinates of the target substance in the color system coordinate space are obtained, and the mesh formed by the intersection of the calibration curves on the multi-component calibration surface from the plurality of color system coordinate spaces is wider than other meshes Select a color system coordinate space that presents a surface and extend the coordinates of the measurement target substance in the color system coordinate space from the position of the multi-component calibration plane along each calibration curve to correspond to the measurement target substance A multi-component quantification method characterized in that each component is quantified by obtaining component values to be determined.   The color system coordinate space is an RGB color system, an XYZ color system, an L * a * b * color system, an L * u * v * color system, or a UCS color system defined by the International Lighting Commission, or 2. The multi-component quantification method according to claim 1, wherein the coordinate system is a color system coordinate space belonging to any of the Hunter Lab color systems.   The multi-component quantification method according to claim 1, wherein the color difference information is measured using a color difference meter.   2. The multi-component quantitative method according to claim 1, wherein a single equilibrium state is established between at least two components contained in the measurement target substance. Measuring means for reading color / color difference information from light irradiation on the measurement target substance, and calibration plane data for storing data of a multi-component calibration plane of at least two components included in the measurement target substance corresponding to a plurality of color system coordinate spaces Corresponding to the color system coordinate space in which the mesh formed by the intersection of the calibration lines on the multi-component calibration surface from the storage means and the plurality of color system coordinate spaces exhibits a calibration surface that is wider than other meshes And calculating means for determining the position of the multi-component calibration surface based on the color difference information, and determining a component value corresponding to the measurement target substance from the position of the multi-component calibration surface. A multi-component quantification device comprising a quantification means for quantifying each component.   The multi-component calibration surface data stored in the calibration surface data storage means includes the multi-component calibration surface data measured using the multi-component quantification device in the past, and the multi-component calibration surface data created before the start of measurement. 6. The multi-component quantitative apparatus according to claim 5, wherein the multi-component quantitative apparatus is one of data or standard data or a combination thereof.

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