JP3656280B2 - How to control filamentous bulking - Google Patents
How to control filamentous bulking Download PDFInfo
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- JP3656280B2 JP3656280B2 JP13874595A JP13874595A JP3656280B2 JP 3656280 B2 JP3656280 B2 JP 3656280B2 JP 13874595 A JP13874595 A JP 13874595A JP 13874595 A JP13874595 A JP 13874595A JP 3656280 B2 JP3656280 B2 JP 3656280B2
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- activated sludge
- filamentous
- bulking
- protin
- protease
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Description
【0001】
【産業上の利用分野】
本発明は、糸状性細菌溶菌酵素による活性汚泥槽の糸状性バルキング防除方法に関する。
【0002】
【従来の技術】
活性汚泥法は、1914年にAndernとLockettらにより開発されて以来、微生物の能力を活用した画期的な廃水処理法として用いられている。この活性汚泥法の利点は、BOD除去率が高く、運転操作が簡単で、自然生態系とも調和する処理方法であるが、その反面、窒素、リン除去率が低いことや、バルキングの発生などの問題点がある。バルキングは、活性汚泥の圧密性が低下し、活性汚泥と処理水の分離が悪化するために処理水とともに活性汚泥が流出する現象で、処理水の水質が悪化することになる。この原因の大半は、糸状性細菌の異常増殖によるとされている。このため、活性汚泥法においては、バルキングの防除および予防が重要な課題となっている。
【0003】
これまで、活性汚泥に発生する糸状性バルキングを防除するために、化学的および微生物学的方法が用いられている。このうち、化学的方法に用いる防除剤としては、塩素剤や第四級アンモニウム塩等の殺菌剤と各種沈降・凝集剤がある。しかし、殺菌剤は、比較的多量に用いることにより、その効果は確実であるが、作用が無差別的であるため、活性汚泥の有用微生物の増殖をも阻害することがあるほか、処理水中にも残存する可能性があり、環境への悪影響が懸念される。沈降・凝集剤は、活性汚泥の凝集および沈降に関与するだけで、糸状性細菌には作用しないため、本質的な解決にならないうえ、連続投入が要求される。その上、高分子凝集剤が使用されている場合は、同時に環境への悪影響も懸念される。微生物学的方法に用いる微生物製剤は、効果が確実ではないし、効果があらわれるまで長い時間を要する場合が多い。これは、微生物製剤中の微生物が糸状性細菌溶菌能もしくは殺菌能を有していても、活性汚泥のような各種微生物が存在する混合系のなかで、しかも生育環境が最適とも言えない条件下で活着させること自体難しいことであるし、ましてやその能力を十分発揮させることはなおさら困難なためである。このため、活性汚泥中の糸状性細菌以外の有用微生物や周囲の環境等に悪影響をおよぼさず、即効性のあるバルキング防除剤が求められている。
【0004】
【発明が解決しようとする課題】
本発明は、有用な微生物等の増殖を妨げることなく、また環境にも悪影響を与えず、即効性のある活性汚泥の糸状性バルキング防除方法を提供せんとすることにある。
【0005】
【課題を解決するための手段】
本発明は、微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼを活性汚泥槽に添加することを特徴とする酵素を用いた糸状性バルキングの防除方法である。
【0006】
本発明者らは、微生物起源で中性もしくはアルカリ性領域で作用するプロテアーゼを探索し、例えばバチルス ズブチルス起源のエンドペプチダーゼで至適pHを中性領域に持つプロテアーゼNアマノ(天野製薬株式会社)、バチルス属起源のエンドペプチダーゼで至適pHをアルカリ性領域に持つGODO−BAP(合同酒精株式会社)、アスペルギルス オリゼ起源のエンドペプチダーゼで至適pHを中性領域に持つプロチンFN(大和化成株式会社)、バチルス ズブチルス起源のエンドペプチダーゼで至適pHをアルカリ性に持つプロチンA(大和化成株式会社)、バチルス属起源のエンドペプチダーゼで至適pHをアルカリ性領域に持つビオサム(昭和電工株式会社)、バチルス属起源のエンドペプチダーゼで至適pHをアルカリ性領域に持つカズサーゼ(昭和電工株式会社)、バチルス属起源のエンドペプチダーゼで至適pHをアルカリ性領域に持つエスペラーゼ(ノボノルディスクバイオインダストリー株式会社)等が糸状性細菌を溶菌することを見いだした。これらのプロテアーゼを活性汚泥槽に添加することにより、活性汚泥の有用な微生物の増殖を阻害せず、糸状性細菌を選択的に溶菌し、バルキングを防除することにより、活性汚泥の沈降性を改善することを見いだした。一般に活性汚泥法では、曝気槽水が中性領域であることが多いため、例えば、バチルス ズブチルス起源のエンドペプチダーゼで、至適pHを中性領域に持つプロテアーゼNアマノ(天野製薬株式会社)やアスペルギルス オリゼ起源のエンドペプチダーゼで、至適pHを中性領域に持つプロチンFN(大和化成株式会社)等が特に有効である。
【0007】
本発明において、微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼを活性汚泥中において100ppm〜10,000ppm、好ましくは、200ppm〜1,000ppmで用いることにより、糸状性バルキングを防除することができる。
【0008】
本発明における微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼが溶菌作用を示すのは、例えば、スフェロチルス属、チオスリックス属、ベギアトア属、さらにEikelboom(Water Res.、第9巻、365−388頁、1975年)によって命名されたTYPE 1701、1702、021N等の糸状性細菌に対してである。特に世界中の廃水処理施設で糸状性バルキングの最も主要な原因となっているTYPE 021Nには非常に有効である。
【0009】
活性汚泥槽に微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼを添加する方法としては、活性汚泥中に直接添加、または、返送汚泥や流入廃水中に混和、混合するなどの方法が適宜とれる。
【0010】
バルキングが発生した活性汚泥槽に微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼを添加すると、糸状性細菌を特異的、即効的に溶菌することができ、効果的にバルキングを防除できる。
【0011】
【実施例】
以下、本発明を実施例により更に具体的に説明するが、本発明は、以下の実施例に何ら限定されるものではない。
【0012】
実施例1(溶菌性試験)
グルコース0.3g/l、酢酸ナトリウム0.3g/l、硫酸アンモニウム0.1g/l、塩化カリウム0.05g/l、硫酸アンモニウム0.05g/l、炭酸カルシウム0.02g/l、塩化第二鉄0.0005g/l、リン酸二ナトリウム0.312g/l、リン酸一ナトリウム0.02g/l、寒天エキス200ml/l、ビタミンミックス* 5ml/l、pH7.0からなる培地に供試糸状性細菌としてTYPE 021N(タイプII−3 T1−4株)を植菌し、30℃にて2〜3日間振盪培養を行った。
(ビタミンミックス* : パントテン酸カルシウム20mg/l、ニコチン酸20mg/l、ビオチン1mg/l、シアノコバラミン1mg/l、葉酸1mg/l、塩酸ピリドキシン20mg/l、パラアミノ安息香酸20mg/l、コカルボキシラーゼ20mg/l、イノシット20mg/l、塩酸チアミン20mg/l、リボフラビン20mg/l、アデニン2mg/l、ウラシル2mg/l、チミン2mg/l)
培養されたTYPE 021N(タイプII−3 T1−4株)を集め、50mMトリス塩酸緩衝液(pH8.5)に懸濁させ、ホモジナイズした。この懸濁液に本発明の微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼのうち、プロテアーゼNアマノ(天野製薬株式会社)、GODO−BAP(合同酒精株式会社)、プロチンFN(大和化成株式会社)、プロチンAS10(大和化成株式会社)、ビオサムAP−1.5(昭和電工株式会社)、カズサーゼAP−2.2(昭和電工株式会社)、エスペラーゼ4.0T(ノボノルディスクバイオインダストリー株式会社)をそれぞれ100ppmとなるように秤取り添加した。添加直後の吸光度を600nmで測定した。続いて、37℃でインキュベーションし、一定時間ごとに吸光度を測定し、TYPE 021N(タイプII−3 T1−4株)の溶菌状態を観察した。なお、対照区としてプロテアーゼNアマノ、GODO−BAP、プロチンFN、プロチンAS10、ビオサムAP−1.5、カズサーゼAP−2.2エスペラーゼ4.0Tを添加していないものを使用した。
菌体残存率は、試験開始時のOD600nm値を100% として求めた値である。
その結果、表1に示した。
【0013】
【表1】
【0014】
表1に示すように、糸状性細菌は、プロテアーゼNアマノ、GODO−BAP、プロチンFN、プロチンAS10、ビオサムAP−1.5、カズサーゼAP−2.2、エスペラーゼ4.0Tを添加することにより、糸状性細菌の残存量が減少し、糸状性細菌が溶菌されていた。
【0015】
実施例2(溶菌性試験)
酵母エキス2.5g/l、ペプトン5.0g/l、グルコース1.0g/l、pH7.0からなる培地に供試細菌として、シュードモナス エルギノーサ(P seudomonas aeruginosa) IFO12689とバチルスズブチリス(Bacillus subtilis) IFO3134、エシェリシア コリ(Escherichia coli) ATCC27166をそれぞれ植菌し、30℃で1〜2日間振盪培養を行った。
培養されたそれぞれの細菌を各種類ごとに別々に集め、50mMトリス塩酸緩衝液(pH8.5)に懸濁させた。この懸濁液に本発明の微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼのうち、プロテアーゼNアマノ、GODO−BAP、プロチンFN、プロチンAS10、ビオサムAP−1.5、カズサーゼAP−2.2、エスペラーゼ4.0Tをそれぞれ1,000ppmとなるように秤取り添加した。添加直後の吸光度を600nmで測定した。続いて、37℃でインキュベーションし、一定時間ごとに吸光度を測定し、それぞれの細菌の溶菌状態を観察した。なお、対照区として、プロテアーゼNアマノ、GODO−BAP、プロチンFN、プロチンAS10、ビオサムAP−1.5、カズサーゼAP−2.2、エスペラーゼ4.0Tを添加していないものを使用した。 菌体残存率は、試験開始時のOD600nm値を100% として求めた値である。
その結果、表1に示したように、各供試細菌ともプロテアーゼNアマノ、GODO−BAP、プロチンFN、プロチンAS10、ビオサムAP−1.5、カズサーゼAP−2.2、エスペラーゼ4.0Tによっては、ほとんど溶菌されなかった。従って、糸状性細菌と一般的な他の細菌との間には、微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼの溶菌性に対して大きな感受性差があることがわかった。
【0016】
実施例3(バルキング防除試験)
グルコース870mg/l、ペプトン250mg/l、塩化ナトリウム300mg/l、リン酸二カリウム39.1mg/l、硝酸アンモニウム43mg/lの合成廃水を調製した。
合成廃水の流入量 1l/日、MLSS 2,000ppm、室温20〜25℃で図1に示す連続培養活性汚泥システムを用いて、活性汚泥の培養を行い、糸状性バルキングを発生させ、以下の実験を行った。なお、このとき発生していた糸状性細菌は、TYPE 021Nとスフェロチルス ナタンスであった。
試験区として、微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼのうち、プロチンFNを曝気槽に対し、400ppmとなるように秤取り、投与した。対照区にはプロチンFNを投与しなかった。その後、対照区、試験区のそれぞれの活性汚泥の沈降度を測定し、顕微鏡観察を行った。なお、活性汚泥の沈降度は、メスシリンダーを用いて、SV180を測定した。
その結果、図2に示したように、プロチンFN投与区の活性汚泥の沈降度は、投与翌日から対照区に比べ、改善された。また、プロチンFNを投与した翌日の両者の活性汚泥を顕微鏡観察したところ、図3に示したように対照区は、糸状性細菌が視野一杯に広がっていたのに対し、図4に示したように試験区は、糸状性細菌はかなり減少していた。また、試験区は活性汚泥のフロックもしっかりしていた。
【0017】
【発明の効果】
微生物起源で中性もしくはアルカリ性領域で作用し、かつ糸状性細菌溶菌能を有するプロテアーゼを活性汚泥槽に添加することにより、活性汚泥の有益な微生物に悪影響を与えることなく、糸状性細菌を特異的、即効的に溶菌することができ、効果的に糸状性バルキングを防除する事ができる。
【図面の簡単な説明】
【図1】実施例3に用いた連続培養活性汚泥システム
【図2】実施例3のプロチンFN投与における活性汚泥沈降度変化
【図3】実施例3の対照区の活性汚泥の顕微鏡写真
【図4】実施例3の試験区の活性汚泥の顕微鏡写真
【符号の説明】
1:滅菌処理済み合成廃水(4.5l)
2:曝気槽(2l)
3:沈殿槽(0.5l)
4:定量ポンプ
5:エアーポンプ
6:滅菌フィルター
7:スターラー
8:処理水[0001]
[Industrial application fields]
The present invention relates to a method for controlling filamentous bulking in an activated sludge tank using a filamentous bacterial lytic enzyme.
[0002]
[Prior art]
Since the activated sludge method was developed by Andern and Lockett et al. In 1914, it has been used as a revolutionary wastewater treatment method utilizing the ability of microorganisms. The advantage of this activated sludge method is a treatment method that has a high BOD removal rate, is easy to operate, and harmonizes with the natural ecosystem, but on the other hand, it has a low nitrogen and phosphorus removal rate and the occurrence of bulking. There is a problem. Bulking is a phenomenon in which activated sludge flows out together with the treated water because the compactness of the activated sludge is reduced and the separation of the activated sludge and the treated water is deteriorated, and the quality of the treated water is deteriorated. Most of this is attributed to abnormal growth of filamentous bacteria. For this reason, in the activated sludge method, control and prevention of bulking are important issues.
[0003]
To date, chemical and microbiological methods have been used to control filamentous bulking that occurs in activated sludge. Among these, as the control agent used in the chemical method, there are bactericides such as chlorinating agents and quaternary ammonium salts and various precipitating / flocculating agents. However, the effect of the disinfectant is certain by using a relatively large amount, but since its action is indiscriminate, it may also inhibit the growth of useful microorganisms in activated sludge, and in the treated water. May remain, and there are concerns about adverse environmental impacts. The sedimentation / flocculant is only involved in the aggregation and sedimentation of activated sludge and does not act on filamentous bacteria. Therefore, the sedimentation / flocculant is not an essential solution and requires continuous input. In addition, when a polymer flocculant is used, there is also a concern about an adverse effect on the environment. Microbial preparations used in microbiological methods are not reliable and often require a long time to appear. This is because the microorganisms in the microbial preparation have the ability to lyse or disinfect filamentous bacteria, but in a mixed system in which various microorganisms such as activated sludge are present, and the growth environment is not optimal. This is because it is difficult to bring it to life, and it is even more difficult to make full use of its ability. For this reason, there is a demand for a bulking control agent that has an immediate effect without adversely affecting useful microorganisms other than filamentous bacteria in the activated sludge and the surrounding environment.
[0004]
[Problems to be solved by the invention]
It is an object of the present invention to provide a method for controlling activated sludge filamentous bulking that has immediate effect without hindering the growth of useful microorganisms and the like and without adversely affecting the environment.
[0005]
[Means for Solving the Problems]
The present invention is a method for controlling filamentous bulking using an enzyme, characterized in that a protease having a microbial origin and acting in a neutral or alkaline region and having a filamentous bacterial lysis ability is added to an activated sludge tank.
[0006]
The present inventors searched for proteases that originate in microorganisms and act in the neutral or alkaline region. For example, protease N Amano (Amano Pharmaceutical Co., Ltd.), Bacillus, which is an endopeptidase derived from Bacillus subtilis and has an optimum pH in the neutral region. Endopeptidase of the genus origin and having an optimum pH in the alkaline region GODO-BAP (Joint Shusei Co., Ltd.), Aspergillus oryzae-derived endopeptidase having the optimum pH in the neutral region (Daiwa Kasei Co., Ltd.) and Bacillus Probutin A (Daiwa Kasei Co., Ltd.), which is an alkaline endopeptidase derived from subtilis, and Biosum (Showa Denko Co., Ltd.), an alkaline endotopeptidase derived from Bacillus, which has an alkaline pH, Optimum pH with alkaline peptidase Kazusaze (Showa Denko KK) having a, Esperase with optimum pH in the alkaline region with endopeptidase Bacillus origin (Novo Nordisk Bioindustry Ltd.) was found to lyse filamentous bacteria. Addition of these proteases to the activated sludge tank improves the sedimentation of activated sludge by selectively lysing filamentous bacteria and controlling bulking without inhibiting the growth of useful microorganisms in activated sludge. I found something to do. In general, in the activated sludge method, since the aeration tank water is often in the neutral region, for example, endopeptidase derived from Bacillus subtilis and protease N Amano (Amano Pharmaceutical Co., Ltd.) and Aspergillus having the optimum pH in the neutral region. Protin FN (Daiwa Kasei Co., Ltd.), which is an endopeptidase derived from oryzae and has an optimum pH in the neutral region, is particularly effective.
[0007]
In the present invention, a protease having a microorganism origin and acting in a neutral or alkaline region and having a filamentous bacterial lysis ability is used in activated sludge at 100 ppm to 10,000 ppm, preferably 200 ppm to 1,000 ppm. Sexual bulking can be controlled.
[0008]
Proteases that act in the neutral or alkaline region and have the ability to lyse the filamentous bacteria in the present invention exhibit the lytic activity. For example, the genus Spherocillus, Thiothrix, Begiatoa, and Eikeboom (Water Res., 9: 365-388 (1975), for filamentous bacteria such as TYPE 1701, 1702, 021N. It is particularly effective for TYPE 021N, which is the most common cause of filamentous bulking in wastewater treatment facilities around the world.
[0009]
As a method of adding a protease having a microbial origin and acting in a neutral or alkaline region and having a filamentous bacterial lysis ability to the activated sludge tank, it can be added directly to the activated sludge, or mixed in the return sludge and influent wastewater. The method of mixing etc. can be taken suitably.
[0010]
Addition of proteases that act in the neutral or alkaline region of microbial origin and have the ability to lyse filamentous bacteria to the activated sludge tank where bulking has occurred, can specifically and quickly lyse filamentous bacteria, Can control bulking.
[0011]
【Example】
EXAMPLES Hereinafter, although an Example demonstrates this invention further more concretely, this invention is not limited to a following example at all.
[0012]
Example 1 (Bacteriolytic test)
Glucose 0.3 g / l, sodium acetate 0.3 g / l, ammonium sulfate 0.1 g / l, potassium chloride 0.05 g / l, ammonium sulfate 0.05 g / l, calcium carbonate 0.02 g / l,
(Vitamin mix * : calcium pantothenate 20 mg / l, nicotinic acid 20 mg / l, biotin 1 mg / l, cyanocobalamin 1 mg / l, folic acid 1 mg / l, pyridoxine hydrochloride 20 mg / l, paraaminobenzoic acid 20 mg / l, cocarboxylase 20 mg / l l, inosit 20 mg / l, thiamine hydrochloride 20 mg / l, riboflavin 20 mg / l,
The cultured TYPE 021N (type II-3 T1-4 strain) was collected, suspended in 50 mM Tris-HCl buffer (pH 8.5), and homogenized. Among these proteases, protease N Amano (Amano Pharmaceutical Co., Ltd.), GODO-BAP (Godoshu Seiko Co., Ltd.), among the proteases that act in the neutral or alkaline region of the suspension of the present invention and have the ability to lyse filamentous bacteria. ), Protin FN (Daiwa Kasei Co., Ltd.), Protin AS10 (Daiwa Kasei Co., Ltd.), Biosum AP-1.5 (Showa Denko Co., Ltd.), Kazusease AP-2.2 (Showa Denko Co., Ltd.), Esperase 4.0T (Novo Nordisk Bio Industry Co., Ltd.) was weighed and added to 100 ppm each. The absorbance immediately after the addition was measured at 600 nm. Then, it incubated at 37 degreeC, the light absorbency was measured for every fixed time, and the lysis state of TYPE021N (type II-3 T1-4 strain) was observed. In addition, the thing which did not add protease N Amano, GODO-BAP, protin FN, protin AS10, biosum AP-1.5, kazuse AP-2.2 esperase 4.0T was used as a control group.
The cell survival rate is a value obtained by setting the OD 600 nm value at the start of the test as 100%.
The results are shown in Table 1.
[0013]
[Table 1]
[0014]
As shown in Table 1, filamentous bacteria are added by adding protease N Amano, GODO-BAP, protin FN, protin AS10, biosum AP-1.5, kazuse AP-2.2, esperase 4.0T. The remaining amount of filamentous bacteria was reduced and the filamentous bacteria were lysed.
[0015]
Example 2 (Bacteriolytic test)
Yeast extract 2.5 g / l, peptone 5.0 g / l, glucose 1.0 g / l, as provided試細bacterium in a medium consisting of pH 7.0, Pseudomonas aeruginosa (P seudomonas aeruginosa) IFO12689 and Bacillus subtilis (Bacillus subtilis) IFO3134 Escherichia coli ATCC27166 was inoculated and cultured at 30 ° C. for 1-2 days with shaking.
Each cultivated bacterium was collected separately for each type, and suspended in 50 mM Tris-HCl buffer (pH 8.5). Among the proteases of the present invention that act in the neutral or alkaline region of the microbial origin of the present invention and have the ability to lyse filamentous bacteria, protease N Amano, GODO-BAP, protin FN, protin AS10, biosum AP-1 .5, Kazusase AP-2.2, and Esperase 4.0T were weighed and added to 1,000 ppm each. The absorbance immediately after the addition was measured at 600 nm. Then, it incubated at 37 degreeC, the light absorbency was measured for every fixed time, and the lysis state of each bacteria was observed. In addition, as a control group, protease N amano, GODO-BAP, protin FN, protin AS10, biosum AP-1.5, kazuse AP-2.2, and esperase 4.0T were not used. The cell survival rate is a value obtained by setting the OD 600 nm value at the start of the test as 100%.
As a result, as shown in Table 1, depending on each of the test bacteria, protease N Amano, GODO-BAP, protin FN, protin AS10, biosum AP-1.5, kazuse AP-2.2, and esperase 4.0T. Almost no lysis. Therefore, there is a large sensitivity difference between the filamentous bacteria and other common bacteria with respect to the lytic properties of proteases that act in the neutral or alkaline region of microbial origin and have the ability to lyse filamentous bacteria. I understood it.
[0016]
Example 3 (bulking prevention test)
A synthetic wastewater was prepared containing glucose 870 mg / l, peptone 250 mg / l, sodium chloride 300 mg / l, dipotassium phosphate 39.1 mg / l and ammonium nitrate 43 mg / l.
Synthetic wastewater inflow 1 L / day, MLSS 2,000 ppm, room temperature 20-25 ° C., using the continuous culture activated sludge system shown in FIG. Went. The filamentous bacteria generated at this time were TYPE 021N and spherocillus natans.
As a test group, protin FN was weighed out and administered to aeration tank to a concentration of 400 ppm among proteases having a microbial origin and acting in a neutral or alkaline region and having the ability to lyse filamentous bacteria. Protin FN was not administered to the control group. Then, the sedimentation degree of each activated sludge of a control group and a test group was measured, and the microscope observation was performed. In addition, SV180 was measured for the sedimentation degree of activated sludge using the graduated cylinder.
As a result, as shown in FIG. 2, the sedimentation degree of the activated sludge in the protin FN administration group was improved from the day after administration compared to the control group. In addition, when the activated sludge on both days after administration of protin FN was observed with a microscope, as shown in FIG. 3, in the control group, filamentous bacteria spread over the entire field of view, whereas as shown in FIG. In the test plot, filamentous bacteria were considerably reduced. In the test area, activated sludge flocs were solid.
[0017]
【The invention's effect】
By adding a protease that has a microbial origin and acts in the neutral or alkaline region and has the ability to lyse filamentous bacteria to the activated sludge tank, the filamentous bacteria can be specifically identified without adversely affecting the beneficial microorganisms of the activated sludge. It can lyse immediately and can effectively control filamentous bulking.
[Brief description of the drawings]
1 is a continuous culture activated sludge system used in Example 3. FIG. 2 is a change in activated sludge sedimentation degree after administration of protin FN in Example 3. FIG. 3 is a micrograph of activated sludge in the control group of Example 3. 4] Photomicrograph of activated sludge in the test section of Example 3 [Explanation of symbols]
1: Sterilized synthetic wastewater (4.5 l)
2: Aeration tank (2l)
3: Precipitation tank (0.5 l)
4: Metering pump 5: Air pump 6: Sterilization filter 7: Stirrer 8: Treated water
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EP2189422B1 (en) | 2007-08-28 | 2015-06-03 | Diamond Engineering Co., Ltd. | Activated sludge material, reduction method of excess sludge amount in bioreactor, and maintenance method of bioreactor |
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