JP3638573B2 - Infusion method of test sample for optical property measurement - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an optical property measuring apparatus used for purity test of a liquid sample, identification of its solute, determination of concentration, etc., in particular, a polarimeter applicable to a urinalysis apparatus. The present invention relates to an infusion device and an infusion method for introduction into a sample cell.
[0002]
[Prior art]
In general, the optical characteristics of the test sample, such as absorbance and optical rotation, are obtained by transmitting light to the test sample held in the sample cell and analyzing the light propagated in the test sample. As the sample cell, a container having a pair of transparent transmission surfaces for transmitting light is used which is a box shape mainly made of glass or the like.
Conventionally, these optical characteristics have been measured by injecting a sample sample into the sample cell from the opened upper portion with a dropper, pipetter, syringe, etc., and then setting the sample cell in the optical system. That is, the introduction and discharge of the test sample and the cleaning of the sample cell have to be performed once the sample cell is removed from the optical system. As described above, the measurement of these optical characteristics is inferior in workability and takes much time. In addition, when bubbles exist in the optical path in the sample cell, the measured values are likely to vary.
[0003]
In recent years, a urine test method using a polarimeter has been proposed. Glucose and protein, ie albumin, show optical rotation. Therefore, the concentration of urine is determined by measuring the optical rotation of urine. According to this method, low concentrations of glucose and protein can be obtained without using consumables such as test paper as in the conventional urinalysis method in which a reagent is immersed in urine and the color reaction is observed with a spectrophotometer or the like. It is possible to detect.
[0004]
[Problems to be solved by the invention]
The present invention is to solve the problems of measure conventional optical properties as described above, the introduction emissions and the test sample, washing the sample cell is easy, the foam is mixed into the test sample in the sample cell and to provide a transportation solution how optical characteristic measuring the Ken試fees to enable highly accurate measurement without interfering with the optical path Te.
[0005]
[Means for Solving the Problems]
The present invention is a container for temporarily housing the test sample liquid for measuring the optical properties, the holding test sample, the sample cell for transmitting the projected light in the test sample, prior to A portion of the container that is immersed in the sample and a conduit that connects the sample cell, a three-way stopcock disposed in the route of the conduit, and a syringe that is connected to one end of the three-way stopcock, The container is connected to the other end through a part of the conduit, the sample cell is connected to the remaining end of the three-way cock through the other part of the conduit, and the syringe is a plunger part. Using an optical property measuring device arranged with the
Injecting a test sample or washing liquid into the container;
Introducing the test sample or the cleaning liquid injected into the container into the syringe through the conduit by sucking using the syringe ; and
Waiting until the movement of bubbles in the test sample or cleaning liquid introduced into the syringe stops;
And a step of introducing the test sample or the cleaning liquid introduced into the syringe into the sample cell through the pipe line by the syringe .
Also, the above the sample cell in a state in which the test sample remained, introducing further said other liquid test sample or washing liquid for measuring an optical characteristic to the sample cell by the steps, remaining on the sample cell It is preferable that the test sample or cleaning liquid is discharged from the sample cell and replaced with the other liquid test sample or cleaning liquid.
[0006]
DETAILED DESCRIPTION OF THE INVENTION
The infusion method for a test sample for optical property measurement according to the present invention includes a container for temporarily storing a liquid sample for measuring optical properties, a test sample, and a projection light on the test sample. A step of injecting a sample or cleaning liquid into a container using a sample cell for permeation, and an optical property measuring apparatus comprising a pipe line connecting the sample cell and a portion in contact with the sample of the container; A step of introducing the test sample or the cleaning liquid injected into the container into the sample cell through a pipe line by suction from the arranged opening.
The test sample in the sample cell is discharged from the sample cell by suction from the opening.
In addition, with the test sample remaining in the sample cell, another liquid test sample or cleaning liquid for measuring optical characteristics is further introduced into the sample cell in each step, and the test sample or cleaning liquid remaining in the sample cell is introduced. Can be discharged from the sample cell and replaced with another liquid test sample or cleaning solution.
[0007]
The infusion device for a test sample for optical property measurement of the present invention is connected to a three-way stopcock for connecting one end to a sample cell holding a liquid test sample for optical property measurement, and to the other end of the three-way stopcock. Equipped with a syringe. For example, a sample container is connected to the other end of the three-way cock.
The infusion apparatus for a test sample for optical property measurement according to the present invention includes a syringe for discharging a sample for connection to a sample cell holding a liquid sample for optical property measurement. Moreover, the sample in a sample cell can be discharged | emitted outside from the open | released end of a three-way cock by connecting between a syringe and a sample cell via a three-way cock.
[0008]
The above infusion device can be applied to, for example, a polarimeter that measures the concentration of fructose, sucrose, glucose and the like in an aqueous solution.
In particular, a monochromatic light source that projects substantially parallel light, a polarizer that transmits only the polarized light component in a specific direction of the substantially parallel light, and a liquid test that is disposed so that substantially parallel light that has passed through the polarizer is transmitted. A sample cell for holding the sample, a coil for applying a magnetic field in the propagation direction of substantially parallel light that passes through the test sample in the sample cell, a current source for passing a current through the coil, and a current for flowing through the coil Magnetic field sweeping means for sweeping, magnetic field modulation means for modulating the current flowing through the coil, an analyzer that transmits only a polarized component in a specific direction out of the light that has passed through the test sample, and light that has passed through the analyzer is detected An optical sensor, a lock-in amplifier for phase-sensitive detection of the output signal of the optical sensor using the vibration modulation signal of the magnetic field modulation means as a reference signal, a test test based on the magnetic field sweep signal of the magnetic field sweep means and the output signal of the lock-in amplifier By using a polarimeter comprising a calculating unit for calculating the optical rotation, easy polarimeter measurement of optical rotation is obtained.
In particular, the concentration of glucose and albumin in urine can be determined easily and with high precision by measuring the optical rotation of urine using this polarimeter. Therefore, it is possible to provide an excellent urinalysis apparatus.
[0009]
【Example】
The infusion device of the present invention can be applied to optical property measuring devices for various liquid samples.
In the following Reference Examples and Examples, a polarimeter is used as an example of an optical property measuring apparatus, and in particular, a magnetic field is applied to light propagating in a sample, and optical rotation by the optically rotatory substance in the sample is compensated by application of the magnetic field. A magnetic field application type polarimeter that determines the concentration of a substance will be described.
[0010]
<< Reference Example 1 >>
The infusion device of this reference example will be described with reference to FIG.
The temporary storage container 1 stores the collected liquid test sample. The sample cell 2 holds the test sample supplied from the temporary storage container 1. With respect to the test sample accommodated in the sample cell 2, light is transmitted in the direction of the arrow in the figure, and the optical characteristics are measured. The sample cell 2 has an optical path length of 50 mm and can hold a test sample of about 5.7 cc.
[0011]
The sample cell 2 is obtained by processing as follows.
First, the side surface in the major axis direction (55 mm in length) of a rectangular parallelepiped aluminum block was machined into a cylindrical shape with a diameter of 17 mm by cutting out both sides leaving a length of 10 mm. Furthermore, a cylindrical space portion having a diameter of 12 mm was formed between the two surfaces in the major axis direction so as to be inclined by about 5.7 degrees (≈tan-1 (5/50)) with respect to the major axis. . Next, circular holes each having a depth of 2.5 mm and a diameter of 22 mm were formed in these surfaces, and a circular glass plate 4 having a thickness of 2.5 mm and a diameter of 22 mm was fitted therein.
A coil 3 for applying a magnetic field to a test sample accommodated in the sample cell 2 is wound around the cylindrical outer periphery of the sample cell 2 cut out. The coil 3 is formed by winding an enameled wire having a diameter of 0.7 mm for 600 revolutions.
The sample cell 2 is provided with a vent 6 having a diameter of 1.0 mm and an introduction / discharge port 5 having a diameter of 2.5 mm at the upper end and the lower end of the cylindrical space.
The introduction / discharge port 5 is connected to the temporary storage container 1 through a pipe 7 having a diameter of 2.5 mm. The temporary container 1 is held by an elevator 8.
[0012]
Hereinafter, the operation method of this infusion apparatus is demonstrated.
First, a test sample is supplied to the temporary storage container 1 from a beaker or the like. When used for monkey supply of a urine test apparatus, urination may be performed directly in the temporary storage container 1.
Here, when the temporary storage container 1 is supplied, the height of the temporary storage container 1 is increased by the elevator 8 so that the liquid level of the supplied test sample is lower than the introduction / discharge port 5 of the sample cell 2. Adjusted. Here, when bubbles are generated in the test sample when the test sample is supplied, it is preferable to wait until the bubbles finish moving upward.
When the rising of the bubbles is completed, the temporary storage container 1 is raised by the elevator 8. At this time, the sample cell 2 is fixed. When the liquid level of the test sample in the temporary storage container 1 becomes higher than the introduction / discharge port 5, the test sample in the temporary storage container 1 is introduced into the sample cell 2 from the introduction / discharge port 5. When the temporary storage container 1 is further raised and the liquid level of the test sample in the temporary storage container 1 becomes higher than the optical path, the optical characteristics of the test sample can be measured. Here, the opening 7a on the temporary storage container 1 side of the pipe 7 is positioned below the liquid level of the test sample in the temporary storage container 1 before and after the test sample is introduced into the sample cell 2. Arranged. That is, as shown to a figure, you may distribute | arrange to the lowest end of the side surface of the temporary storage container 1, and may distribute | arrange to the location away from the lower end of the side surface as long as the bottom surface or the conditions are satisfy | filled.
When the test sample is introduced into the sample cell 2, the air in the sample cell 2 is discharged from the vent 6. In particular, when the test sample is introduced from the lowermost end of the sample cell 2 by giving an inclination to the axis of the cylindrical space portion, the test sample is supplied to the sample cell 2 more smoothly. This further prevents bubbles from being mixed into the test sample in the sample cell 2.
[0013]
When the test sample in the sample cell 2 is discharged, the temporary storage container 1 is lowered, and the test sample in the sample cell 2 is returned to the temporary storage container 1 from the introduction / discharge port 5. At this time, air flows into the sample cell 2 from the vent 6.
When the inside of the sample cell 2 is cleaned, water or a cleaning liquid may be put into the temporary storage container 1 and introduced into the sample cell 2 and discharged in the same manner as described above.
When exchanging the test sample in the sample cell 2, if there is a sufficient amount of the test sample, a new test sample is put in the temporary container 1 and moved to the sample cell 2 in the same manner as described above. The previous test sample in the inside may be discharged from the vent 6 and thus the test sample may be replaced. The same applies to the cleaning.
[0014]
With the above configuration, the test sample can be introduced without mixing bubbles in a sample cell previously installed in the optical system. Also, it is not necessary to remove the cell from the optical system when discharging the test sample from the sample cell, replacing the test sample, or cleaning the cell. Therefore, highly accurate optical characteristics can be measured, and workability is greatly improved.
[0015]
Example 1
The outline of the infusion apparatus of a present Example is shown in FIG.
The sample cell 2 used in this example is the same as that used in Reference Example 1. However, the other end of the pipe 17 connected to the introduction / discharge port 5 is connected to the end c of the three-way cock 10. Further, the end b of the three-way cock 10 is connected to the syringe 9, and the end a is connected to the funnel 11 through the pipe 18.
First, the test sample is put into the funnel 11. When used as a urine test apparatus, urination may be performed directly in the funnel 11. Next, the test sample is sucked into the syringe 9 through the ab of the three-way stopcock 10.
[0016]
Here, when the test sample is inhaled into the syringe 9, if bubbles are generated in the test sample in the syringe 9, the process waits until the bubbles move upward and stop. When stopped, the three-way cock 10 is passed through bc, and the syringe 9 is further driven to introduce the test sample into the sample cell 2. After introducing the test sample until the liquid level becomes higher than the optical path 4, the measurement is performed. In particular, by arranging the syringe 9 with the plunger portion facing upward, the test sample can be introduced into the sample cell 2 while collecting the bubbles of the test sample in the syringe 9. Thereby, it can prevent that a bubble mixes in the test sample introduce | transduced into the sample cell 2. FIG.
When the test sample is discharged from the sample cell 2, the test sample in the sample cell 2 is sucked into the syringe 9 through the bc of the three-way cock 10, and then is discharged through the ab into the funnel 11. When the sample cell 2 is cleaned, water or a cleaning liquid is put into the funnel 11 and then moved to the sample cell in the same manner as described above, and is discharged after cleaning. Similarly to Reference Example 1, a new test sample or cleaning liquid may be sent to a sample cell containing the test sample and replaced with the previous test sample or cleaning liquid.
[0017]
Note that the temporary storage container 1 of Reference Example 1 may be used instead of the funnel 11.
Further, for example, a rubber tube may be used for the pipe 18 and the opening end may be immersed in a bowl containing the test sample.
Further, even if the three-way stopcock 10 is not used, the connection between the syringe 9 and the pipe 17 is appropriately cut off, and a test liquid or a cleaning liquid such as water is directly collected by the syringe 9 or is discharged from the sample cell 2. The same effects as described above can be obtained.
As described above, in the infusion device of the present embodiment, the syringe 9 substantially fulfills the functions of the temporary container 1 and the elevator 8 in the infusion device of Reference Example 1.
[0018]
<< Reference Example 2 >>
The infusion device of this reference example is shown in FIG.
In the infusion device of this reference example, the same sample cell 2 as that used in Reference Example 1 is used. However, a suction port 12 having a diameter of 2.5 mm is formed instead of the ventilation port 6.
The infusion device of this reference example uses the same syringe 9, three-way cock 10 and funnel 11 as those used in Example 1 . The suction port 12 of the sample cell 2 is connected to the end a of the three-way cock 10 by a pipe 14. An end b of the three-way cock 10 is connected to the syringe 9. The introduction / discharge port 5 of the sample cell 2 is connected to the funnel 11 and the pipe 13.
[0019]
The test sample is supplied to the funnel 11. Moreover, when using it for a urinalysis apparatus, you may urinate directly to the funnel 11.
The test sample of the funnel 11 is introduced into the sample cell 2 by passing through the ab of the three-way cock 10 and sucking with the syringe 9. Measurement is performed after the liquid level of the test sample in the sample cell 2 is made higher than the optical path.
With the above configuration, even if bubbles generated when passing the pipe 13 when the test sample is poured into the funnel 11 are mixed into the sample cell 2, the bubbles are further sucked into the syringe 9 by being sucked by the syringe 9. Can be moved. After the bubbles sucked into the syringe 9 reach the upper surface of the test sample and stop, the test sample is reintroduced into the sample cell, so that the mixing of the bubbles into the sample cell 2 can be prevented.
[0020]
When the test sample is discharged from the sample cell 2, the test sample in the sample cell 2 is once sucked by the syringe 9 while passing through the ab of the three-way cock 10 and then passed between bc of the three-way cock 10 The test sample in the syringe 9 is discharged from the end c. Here, if the discharge cannot be completed in one operation, the above operation may be repeated.
When the inside of the sample cell 2 is cleaned, water or a cleaning solution is injected into the funnel 11 and then supplied to the sample cell 2 as in the case of the test sample, and after cleaning, it is discharged. In addition, water or cleaning liquid is introduced into the syringe 9 from the end c of the three-way cock 10 through the bc of the three-way cock 10, and then introduced into the sample cell 2 from the syringe 9 through the ab of the three-way cock 10. You may do it.
The three-way stopcock 10 is not necessarily required. For example, the syringe 9 may be driven to discharge the test sample in the sample cell 2 to the funnel 11 side.
[0021]
<< Reference Example 3 >>
This reference example demonstrates the case where the infusion apparatus of the reference example 2 is used for sample supply of a polarimeter.
The configuration of the polarimeter of this reference example is shown in FIG.
The semiconductor laser projection module 15 projects laser light having a wavelength of 780 nm as elliptical substantially parallel light having a major axis of about 4 mm and a minor axis of about 2 mm. The semiconductor laser projection module 15 continuously oscillates the semiconductor laser projected by the built-in semiconductor laser drive circuit.
Of the light projected from the semiconductor laser projection module 15, the polarizer 23 transmits only a polarized light component in a specific direction, for example, light having a polarized light component parallel to the paper surface.
The sample cell 2 receives the light projected from the semiconductor laser projection module 15 and transmitted through the polarizer 23, and propagates the light inside.
The analyzer 24 transmits a polarized light component in a specific direction out of the light transmitted through the sample cell 2. Here, the analyzer 24 is in a state of orthogonal Nicols with the polarizer 23. That is, when the polarizer 23 transmits only light having a polarization component parallel to the paper surface, the analyzer 24 is disposed so as to transmit only light having a polarization component perpendicular to the paper surface. The optical sensor 25 detects light transmitted through the analyzer 24.
[0022]
The computer 20 issues a command signal to the current source 19 to sweep the current flowing through the coil 3 in the range of -5 to 5A. On the other hand, the signal generator 21 supplies a 1.3 kHz vibration modulation signal to the current source 19. The current source 19 converts the vibration modulation signal from the signal generator 21 into a vibration modulation current signal having an amplitude of 0.02 A, and further superimposes the vibration modulation signal on the sweep current instructed by the computer 20, and then supplies this to the coil 3.
The lock-in amplifier 22 performs phase sensitive detection on the output signal of the optical sensor 25 using the vibration modulation signal of the signal generator 21 as a reference signal. Since the output signal of the lock-in amplifier 22 corresponds to the angular frequency component of the output signal of the optical sensor 25, the extinction point is when the output signal of the lock-in amplifier 22 becomes zero.
The computer 20 records and analyzes the output signal of the lock-in amplifier 22.
[0023]
Actually, the optical rotation of a sucrose aqueous solution having a temperature of 20 ° C., a pure water and a concentration of 250 mg / dl was measured using the polarimeter.
FIG. 5 shows an output signal of the lock-in amplifier 22 when the current flowing through the coil 3 is swept in the range of −1.5 to 1.5 A. In FIG. 5, the horizontal axis is the current J flowing through the coil 3, and the vertical axis is the output signal (arbitrary value) of the lock-in amplifier 22.
In the figure, the solid line indicates the measurement result of pure water that does not show optical rotation. The extinction point is when J is zero. This is a state in which the magnetic field is not applied to the pure water as the test sample, and the polarization direction is not rotated by the optical Faraday effect.
On the other hand, the dotted line in the figure is the measurement result of the sucrose aqueous solution. In this case, the extinction point is when J is 1.21A. That is, it is a straight line obtained by translating the solid line by + 1.21A width. The deviation width of the extinction point corresponds to the optical rotation of the test sample.
[0024]
Furthermore, sucrose aqueous solutions having concentrations of 50, 100, 150 and 250 mg / dl were measured in order using the above polarimeter at a temperature of 20 ° C.
First, an aqueous sucrose solution having a concentration of 50 mg / dl was poured into the funnel 11, sucked with a syringe 9, introduced into the sample cell 2, and the optical rotation was measured. When the measurement was completed, the sucrose aqueous solution in the sample cell 2 was sucked into the syringe 9 and discharged from the end c of the three-way cock 10. Next, water was poured into the funnel 11 and this water was introduced into the sample cell 2 to clean the sample cell 2. After discharging this water, an aqueous sucrose solution having a concentration of 100 mg / dl was poured into the funnel 11 and similarly introduced into the sample cell 2 to measure the optical rotation.
The result is shown in FIG. In the figure, the horizontal axis is the concentration, and the vertical axis is the current J that becomes the extinction point. As is clear from the figure, it was confirmed that the concentration and the measured value were approximated by a linear equation. From this, according to the polarimeter of a present Example, mixing of the bubble to a sample cell can be prevented, and a highly accurate measurement is attained. In addition, a large number of test samples can be efficiently measured by cleaning the inside of the cell.
As described above, the polarimeter of this reference example can measure the glucose concentration with high accuracy. Similarly to glucose, albumin showing optical activity can be measured with high accuracy. That is, by using it for urinalysis, it is possible to accurately grasp the urine sugar value and the urinary albumin concentration. Further, since the test sample can be easily replaced and the cell can be easily cleaned, the work load on the user can be greatly reduced.
In addition, as a means to move a test sample, you may use a pump other than the elevator and syringe used in the said Example.
[0018]
【The invention's effect】
According to the present invention, it is possible to provide an infusion device for an optical measuring device that is inexpensive and has high workability. In addition, since this infusion device can avoid the introduction of bubbles during the introduction of the test sample into the sample cell, the use of this infusion device provides a compact and easy-to-use polarimeter and urinalysis device at low cost. It becomes possible to do.
[Brief description of the drawings]
FIG. 1 is a schematic view showing a configuration of an infusion device according to a reference example of the present invention.
Is a schematic view showing the arrangement of the infusion device of one embodiment of the present invention; FIG.
FIG. 3 is a schematic view showing a configuration of an infusion device according to another reference example of the present invention.
FIG. 4 is a schematic diagram showing the configuration of a polarimeter of one reference example of the present invention.
FIG. 5 is a characteristic diagram showing a relationship between a current J flowing through a coil and an output signal of a lock-in amplifier in pure water and a sucrose aqueous solution obtained using the polarimeter.
FIG. 6 is a characteristic diagram showing the relationship between the concentration of an aqueous sucrose solution and the current value J at which the extinction point appears in the same polarimeter.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Temporary storage container 2 Sample cell 3 Coil 4 Glass plate 5 Introduction discharge port 6 Ventilation port 7 Piping 8 Elevator 9 Syringe 10 Three-way stopcock a, b, c End part 11 Funnels 13, 17, 18 Piping 15 Semiconductor laser projection module 17, 18 piping 19 current source 20 computer 21 signal generator 22 lock-in amplifier 23 polarizer 24 analyzer 25 optical sensor

Claims (2)

Container for temporarily housing the test sample liquid for measuring the optical properties, the holding test sample, the sample cell for transmitting the projected light in the test sample, wherein the pre-SL container A pipe connecting the sample cell and a place immersed in the sample, a three-way cock arranged in the path of the pipe, and a syringe connected to one end of the three-way cock, and at the other end of the three-way cock The container is connected through a part of the conduit, the sample cell is connected to the other end of the three-way cock through the other part of the conduit, and the syringe has a plunger portion upward. Using the arranged optical property measurement device,
Injecting a test sample or washing liquid into the container;
Introducing the test sample or the cleaning liquid injected into the container into the syringe through the conduit by sucking using the syringe ; and
Waiting until the movement of bubbles in the test sample or cleaning liquid introduced into the syringe stops;
Introducing the test sample or the cleaning liquid introduced into the syringe into the sample cell through the conduit with the syringe, and a method for injecting the test sample for optical property measurement. In a state where the test sample remains in the sample cell, another liquid test sample or a cleaning solution for measuring optical characteristics is further introduced into the sample cell through the steps, and the test sample remaining in the sample cell is introduced. The method for injecting a test sample for optical property measurement according to claim 1, wherein the test sample or the cleaning liquid is discharged from the sample cell and replaced with the other liquid test sample or the cleaning liquid .

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