JP3611648B2 - microscope - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a microscope capable of arbitrarily selecting a retracting direction of an objective lens attached to a revolver in an observation state.
[0002]
[Prior art]
As a conventional microscope with a revolver, one having a configuration as shown in FIG. 6 is known.
In FIG. 6, the frame 1 is composed of a column 3 provided perpendicular to the base 2 and an arm 4 provided in parallel with the base 2 at the top of the column 3. Etc. are fixed integrally. Ribs 5 for securing strength are provided on both sides of the lower surface of the arm 4.
[0003]
A lens barrel 7 and a revolver 9 to which an eyepiece 6 is attached are detachably attached to the distal end portion of the arm 4. Further, a photographic device 10 is mounted on the lens barrel 7, and a revolver 9 is provided with a rotating ring 9a having a plurality of objective lenses 8 so that the rotating ring 9a can be rotated with its rotational center deviating from the optical axis. The objective lens 8 is mounted on the same optical axis as the eyepiece 6 by turning the rotating ring 9a.
[0004]
A guide 11 is attached to the column 3, and the stage receiver 14 can be moved in the vertical direction by turning a focusing handle 12 a provided on the base 2. A stage receiver 14 having an XY stage 13 fixed thereto is attached to the guide 11. A capacitor 15 is attached to one side surface of the stage receiver 14 so as to be movable up and down below the XY stage 13.
[0005]
In the figure, 16 is a lamp house provided at the lower back of the column 3.
In this type of microscope with a revolver, as an observation method, a power switch (not shown) is turned on with a sample placed on the XY stage 13 in advance, and the lamp house 16 is turned on.
[0006]
Next, the focusing handle 12a is turned to move the XY stage 13 up and down to focus, and the knob 12b is turned to move the capacitor 15 up and down to the optimum position. By the way, the mounting direction of the objective lens 8 is forward, backward, or vice versa, but it is generally backward from the viewpoint of easy viewing of the sample, and forward from the viewpoint of securing the back space. .
[0007]
As described above, as a microscope that can change the mounting direction of the revolver 9 forward or backward according to the purpose of use, a microscope having a structure in which the revolver 9 can be inserted and removed by an ant guide is known.
[0008]
In this type of microscope, as described above, when the retracting direction of the objective lens 8, that is, the direction of the revolver is changed to a forward or backward displacement, the lens barrel 7 is once removed from the arm 4 and the direction of the revolver is reversed. It is necessary to reattach it.
[0009]
The revolver can be linearly moved in the longitudinal direction of the arm by the dovetail guide, but the revolver cannot be rotated eccentrically around the optical axis.
For general observation, the retracting direction of the objective lens is sufficient for forward or backward.However, when observation is performed at a high level while inserting physiological and electrical instruments from the left and right while observing, for the lens may interfere with the instruments, each time you change the observation magnification must let save these instruments outside the rotation region of the pair objective lens.
[0010]
Therefore, recently, a microscope having a structure as described in Japanese Patent Laid-Open No. 3-100509 is considered as a revolver-replaceable microscope.
FIG. 7 is a sectional view showing the mounting structure of the revolver of such a microscope.
[0011]
In FIG. 7, a revolver 31 is configured by rotatably holding a rotation operation member 31c and a lens attachment member 31d fixed by screwing an objective lens 32a, 32b on a fixing member 31a having an attachment mount 31b. Yes.
[0012]
In order to attach the revolver 31 having such a configuration to the arm 33, the revolver receiver 34 is placed on a placement surface orthogonal to the optical axis 35 formed on the arm 33, and fixed by screws 36a and 36b.
A lens barrel 37 is detachably mounted on the revolver receiver 34.
[0013]
[Problems to be solved by the invention]
However, in the microscope having the configuration shown in FIG. 7, in order to change the retracting direction of the objective lens, the revolver 31 must be rotated with the lens barrel 37 removed and the screws 36a and 36b removed from above. Operation is troublesome.
[0014]
Japanese Patent Laid-Open No. 3-100509 discloses that when the revolver 31 interferes with the rib when the revolver 31 is eccentrically rotated about the optical axis, the revolver 31 is pulled out from the revolver receiver 34. It is disclosed that the revolver is rotated 180 ° around the virtual optical axis and then inserted into the revolver receiver 34 again.
[0015]
However, in such a configuration, when the direction of the revolver is reversed, the revolver must be removed from the arm one by one.
In order to solve the above problems, the present invention enables the revolver to be rotated about the optical axis and fixed at an arbitrary angle, so that the revolver can be freely set at a desired eccentric angle position, easily and to provide operability have good microscope can rotate the revolver.
[0016]
[Means for Solving the Problems]
The present invention for achieving the above object, constitutes a by Ri microscope in the following means.
The invention corresponding to claim 1 includes a frame in which a column is implanted in a base and an arm extending horizontally in the upper part of the column, a revolver provided with a plurality of objective lenses, A through-hole provided at the tip, and a rotation mechanism that is inserted into the through-hole of the arm, and is capable of rotating the revolver eccentrically about the optical axis and rotatably holding the rotation center coincident with the optical axis;
An eccentric angle of the revolver can be arbitrarily set by screwing into the screw hole and a screw hole formed in the arm tip so as to pass through the through hole from the arm tip in a direction perpendicular to the optical axis. And a fixing screw for fixing.
[0017]
The invention corresponding to claim 2 is the invention corresponding to claim 1, wherein reinforcing ribs for reinforcing the arms of the frame are provided outside the rotation region of the revolver rotated by the rotation mechanism.
[0018]
In the invention corresponding to claim 3, a column is implanted in the base, an arm extending in the horizontal direction is provided on the upper portion of the column, and a frame formed by reinforcing the arm with a reinforcing rib; In a microscope in which a revolver support equipped with a revolver equipped with a plurality of objective lenses is provided in the column so as to be movable up and down , a through hole provided in a tip portion of the revolver support,
A rotation mechanism that is inserted into the through hole of the revolver support, and that is capable of rotating the revolver eccentrically about the optical axis and holding the rotation center in alignment with the optical axis, and a direction orthogonal to the optical axis In addition, the eccentric angle of the revolver is arbitrarily fixed by screwing into the screw hole and the screw hole formed at the distal end portion of the revolver support body so as to pass through the through hole from the distal end portion of the revolver support body. Fixing screws.
[0019]
Therefore, in the microscope with a revolver according to the first aspect of the present invention, the revolver can be rotated about the optical axis with the revolver attached, and can be fixed at an arbitrary angle. Can be freely set to a desired eccentric position.
[0020]
In the microscope with a revolver according to the second aspect of the invention, in addition to the above-described effects, the rib for reinforcing the arm is provided outside the rotation region of the revolver rotated by the rotation mechanism. At the time of rotation, it is possible to rotate without touching the rib, and the eccentric position of the revolver can be easily set without removing the revolver from the revolver receiver.
[0021]
Furthermore, in the microscope of the invention corresponding to claim 3, rotatably mounted on the rotation about the revolver to a support mounted to be movable up and down the column to match the optical axis, and a revolver By positioning it below the arm so that the rib does not enter the rotation region, it is possible to easily set the desired rotational eccentric position with the revolver attached, and to rotate the revolver without hitting the rib. .
[0022]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be described below with reference to the drawings.
FIG. 1 is a side view showing a first embodiment of a microscope with a revolver according to the present invention, FIG. 2 is a view taken along arrow A in FIG. 1, and FIG. 3 shows a mounting structure of a revolver with respect to an arm in the same embodiment. In the detailed diagram, the same parts as those in FIGS.
[0023]
1 and 2, the frame 1 is composed of a column 3 provided perpendicular to the base 2 and an arm 4 provided on the top of the column 3 in parallel with the base 2, and the base 2, the column 3 and the arm. 4 is integrally fixed with a screw or the like. In this case, the arm 4 is provided with a rib 5-1 for ensuring strength. The ribs 5-1 for ensuring the strength of the arm 4 is of a short length l ₂ than the length l ₁ of the conventional rib 5 is used.
[0024]
A lens barrel 7 to which an eyepiece 6 is attached is detachably attached on the distal end portion of the arm 4, and a photographic device 10 is further attached on the lens barrel 7.
A revolver 9 is attached below the tip of the arm 4 so as to be eccentrically rotatable about the optical axis. The revolver 9 has a rotating ring 9 a having a plurality of objective lenses 8. By rotating the rotating ring 9 a, the desired objective lens 8 can be placed on the same optical axis as the eyepiece 6. ing.
[0025]
Further, a guide 11 is attached to the front surface of the column 3 and can be moved in the vertical direction by turning a focusing handle 12a provided on the base 2. A stage receiver 14 to which an XY stage 13 is fixed is attached to the guide 11. A capacitor 15 is provided below the XY stage 13, and the capacitor 15 is attached to the stage receiver 14 so as to be movable in the vertical direction.
[0026]
In the figure, 16 is a lamp house provided at the lower back of the column 3.
Here, the details of the mounting structure between the tip of the arm 4 and the revolver 9 will be described with reference to FIG.
[0027]
As shown in FIG. 3, a cylindrical rotating body 17 having a flange portion is fixed to the upper surface of the revolver 9 with screws or the like. The male dovetail portion of the revolver 9 is fitted into the dovetail groove formed in the flange portion of the rotating body 17.
[0028]
The revolver 9 is fixed to the rotating body 17 by tightening the screws 21 from the end face side of the rotating body 17.
The cylindrical portion of the rotating body 17 is inserted into the through hole 4a having an annular step portion on the upper surface portion provided at the distal end portion of the arm 4, and the mounting plate 20 and the rotating body inserted into the annular step portion. The revolver 9 is rotatably held with its rotation center coinciding with the optical axis by coupling 17 to the screw 19.
[0029]
Further, the eccentric angle of the rotating body 17 can be arbitrarily fixed by screwing a screw 18 into a screw hole in a direction orthogonal to the optical axis passing through the through hole 4a from the end face side of the arm 4. .
[0030]
In the microscope with a revolver having such a configuration, as an observation method, a power source (not shown) is turned on with the sample placed on the XY stage 13, and the lamp house 16 is turned on.
[0031]
Next, the focusing handle 12a is turned to move the XY stage 13 up and down to focus, and the capacitor 15 is moved up and down to the optimum position. In the drawing, the direction of the revolver 9 is set backward. In order to change the direction of the revolver 9, the fixing screw 18 is loosened, the revolver 9 is eccentrically rotated about the optical axis, and the fixing screw 18 is tightened and fixed again at an arbitrary position to be fixed.
In the microscope with a revolver according to the first embodiment, since the orientation of the revolver 9 is determined with the manipulator such as a sample, a glass pipette, and an electrode set, the setting efficiency is improved. In this case, by attaching the revolver 9 backward, the objective lens 8 rotates behind the optical axis at the time of magnification conversion. Therefore, if the revolver 9 is installed in front of a peripheral device such as a microcomputer, it will not interfere with the objective lens. In addition, the same product can be used for optimum settings and revolvers (forward and backward) in each field, which is advantageous in terms of cost.
[0033]
Further, since the rib 5-1 for reinforcing the arm 4 has a size and a length that do not enter the rotation region of the revolver 9, the revolver 9 is rotated without being applied to the rib 5-1 when rotating eccentrically. Therefore, the revolver 9 can be easily set to a desired eccentric rotation position without removing the revolver 9 from the rotating body 17.
[0034]
In the first embodiment described above, the mounting direction of the revolver 9 is set rearward, but the direction of the revolver 9 is set to the right side as in the second embodiment described later, for example, according to the arrangement state of peripheral devices and the like. It is possible to set an arbitrary eccentric rotation position so that it can be rotated by 90 °.
[0035]
In the first embodiment, the rotating body 17 is fitted to the through hole in the tip of the arm 4 and can be rotated by sliding. However, the rotating body 17 can be rotated to the arm 4 by a bearing, a metal bearing or the like. You may make it attach.
[0036]
The revolver 9 is detachably attached to the rotating body 17 with screws 21. However, the revolver 9 may be fixed with bolts or fixed with screws after being fitted.
Further, in the figure, the rotating body 17 is located between the arm 4 and the revolver 9, but the present invention can be similarly applied to a type in which the rotating body 17 is built in the revolver or the arm.
[0037]
FIGS. 4 to 5 show a second embodiment of the microscope with a revolver according to the present invention. FIG. 4 is a front view and FIG. 5 is a side view. 4 and 5, the same parts as those in FIG. 1 are denoted by the same reference numerals, and the description thereof is omitted. Only different points will be described here.
[0038]
In the second embodiment, as shown in FIGS. 4 to 5, the stage receiver 25 is fixed to the base 2, and a guide movable in the vertical direction by rotating the focusing handle 12a provided on the base 2 side. 11, a revolver support 26 is fixed, and a revolver 9 is attached to the tip of the revolver support 26 in the same configuration as in the first embodiment.
[0039]
Further, one end of the support member 24 is vertically attached to the front end of the base 2, one end of the fixed stage 28 disposed horizontally toward the column 3 side is attached to the other end of the support member 24, and the other end is attached to the column 3. And firmly attached together with the fixing member 27.
[0040]
A sample case 22 filled with a solution is placed on the XY stage 13 provided on the stage receiver 25, and cells 23 are accommodated in the solution.
Reference numeral 29 denotes a manipulator that is placed on the fixed stage 28 and has an electrode that can suck cells at the tip.
[0041]
In the microscope with a revolver having such a configuration, in order to observe the sample, first, the lamp house 16 is turned on by turning on the power switch, and the focusing handle 12a is rotated to move the objective lens 8 up and down to focus. And the knob 12b is turned to adjust the capacitor 15 to the optimum position.
[0042]
Further, the XY stage 13 is moved in the X and Y directions by the operation handle, and the sample case 22 is set to a position where it is desired to observe. When the positioning of the cell to be clamped is completed in this way, the objective lens is retracted upward by the focusing handle 12a, and the manipulator 29 is placed on the fixed stage 28 in order to insert the electrode near the cell 23.
[0043]
The manipulator 29 is firmly fixed on the fixed stage 28 by a magnet, a screw or the like, and in this state, the objective lens 8 is lowered to focus again. The observer will capture the cells and electrodes as images.
[0044]
In order to clamp the cell with the electrode, the manipulator 29 is operated to bring the electrode close to the cell, and the clamping is terminated by suctioning the contacted portion.
The magnification of the objective lens is 4 to 10 times for cell selection, and 20 to 40 times depending on the cell size (diameter 5 μm to 50 μm) during clamping.
[0045]
In the second embodiment has a length of the ribs 5-2 for reinforcing the arm 4 and l _3.
Since the case of the second embodiment revolver support 26 is disposed below the arm 4, as compared with the first embodiment the length of the ribs 5-2 l ₃ can be further increased .
[0046]
4 to 5, assuming that the manipulator 29 is set on the left side on the stage 28, the fixing screw 18 is loosened before setting the manipulator 29, and the revolver 9 is rotated 90 ° eccentrically about the optical axis to the right. The fixing screw 18 was tightened and fixed with the revolver 9 facing right. Thus, if the direction of the revolver 9 is set on the side opposite to the side on which the manipulator 29 is set, the setting space for the manipulator 29 can be increased.
[0047]
In addition, when observing a biological specimen such as a general slide glass, the direction of the revolver may be set to the front side and the rear side.
As shown in FIG. 4, if the manipulator is arranged on the opposite side (left side) to the revolver direction (right side) and the needle or electrode is set outside the rotation area of the objective lens as shown in the figure, the objective can be changed during objective conversion. The lens does not interfere with the electrode of the manipulator 29. Furthermore, when the electrode is inserted from the right side of the figure, the revolver may be rotated 180 ° around the optical axis from this state and set to the left.
[0048]
As described above, in the microscope with a revolver according to the second embodiment, the revolver is attached to the revolver support that is attached to the column 3 so as to be movable up and down so that the center of rotation coincides with the optical axis. In addition, by positioning the revolver 9 below the arm so that the rib does not enter the rotation area of the revolver, the angle of the revolver 9 can be easily set with the revolver attached, and the revolver 9 is not applied to the rib 5-2. Can be rotated.
Furthermore, by installing the manipulator 29 on the fixed stage 28, the manipulator 29 will not move even if the stage 13 is moved in the X and Y directions. If the needle or electrode can be moved back and forth in accordance with the optical axis, manipulation can be performed without readjustment even when other cells are selected by operating the X and Y stages.
[0050]
【The invention's effect】
As described above, according to the present invention, the revolver can be rotated about the optical axis and fixed at an arbitrary angle, so that the mounting direction of the revolver can be freely set to a desired eccentric position while the revolver is mounted. setting is possible, we can provide operability have good microscope can easily rotate the revolver.
[Brief description of the drawings]
FIG. 1 is a side view showing a first embodiment of a microscope with a revolver according to the present invention.
2 is a view of the revolver of FIG. 1 as viewed from the direction of arrow A. FIG.
FIG. 3 is a detailed view showing a mounting structure of a revolver and an arm in the same embodiment.
FIG. 4 is a front view showing a second embodiment of the present invention.
FIG. 5 is a side view of the same embodiment.
FIG. 6 is a side view showing a conventional microscope with a revolver rotating device.
FIG. 7 is a view showing a main part of a conventional revolver-replaceable microscope.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Frame, 2 ... Base, 3 ... Column, 4 ... Arm, 5-1, 5-1 ... Rib, 6 ... Eyepiece, 7 ... Lens barrel, 8 ... Objective lens, 9 …… Revolver, 9a …… Rotating ring, 10 …… Photo equipment, 11 …… Guide, 12a …… Focusing handle, 12b …… Knob, 13 …… XY stage, 14 …… Stage holder, 15 …… Condenser, 16 ... Lamp house, 17 ... Rotating body, 18 ... Fixed screw, 19 ... Screw, 20 ... Mounting plate, 21 ... Screw, 22 ... Sample case, 23 ... Cell, 24 ... Support member , 25... Stage receiver, 26... Revolver support, 27... Fixed member, 28... Fixed stage, 29.

Claims (3)

A frame in which a column is implanted in the base and an arm extending horizontally is provided at the top of the column ;
A revolver with a plurality of objective lenses;
A through hole provided at the tip of the arm;
A rotation mechanism that is inserted into the through-hole of the arm, and that is capable of rotating the revolver eccentrically about the optical axis and rotatably holding the rotation center coincident with the optical axis;
A screw hole formed at the tip of the arm so as to pass through the through hole from the tip of the arm in a direction perpendicular to the optical axis;
A fixing screw for arbitrarily fixing the eccentric angle of the revolver by screwing into the screw hole;
A microscope comprising: In microscope according to claim 1,
Microscope characterized in that provided outside the rotation region of the revolver to rotate the reinforcing rib for reinforcing the arm of the frame by the rotation mechanism. A column is formed in the base, and an arm extending horizontally is provided at the top of the column, and the arm is reinforced with reinforcing ribs;
In a microscope in which a revolver support body equipped with a revolver having a plurality of objective lenses at the tip is provided on the column so as to be movable up and down,
A through hole provided at the tip of the revolver support;
A rotation mechanism that is inserted into the through hole of the revolver support, and is capable of rotating the revolver eccentrically about the optical axis and holding the rotation center in alignment with the optical axis;
A screw hole formed in the tip portion of the revolver support so as to pass through the through hole from the tip portion of the revolver support in a direction perpendicular to the optical axis;
A fixing screw for arbitrarily fixing the eccentric angle of the revolver by screwing into the screw hole;
A microscope comprising:

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