JP3596777B1 - Health foods and oral antitumor agents - Google Patents
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- 235000013402 health food Nutrition 0.000 title claims abstract description 23
- 239000002246 antineoplastic agent Substances 0.000 title claims abstract description 13
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 43
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 43
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 43
- 241001330002 Bambuseae Species 0.000 claims abstract description 41
- 239000011425 bamboo Substances 0.000 claims abstract description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000284 extract Substances 0.000 claims abstract description 31
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 21
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 21
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 21
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 17
- 239000000126 substance Substances 0.000 claims abstract description 7
- 241000287828 Gallus gallus Species 0.000 claims description 16
- 239000004480 active ingredient Substances 0.000 claims description 2
- 239000005428 food component Substances 0.000 claims description 2
- 230000000259 anti-tumor effect Effects 0.000 abstract description 18
- 235000013305 food Nutrition 0.000 abstract description 6
- 238000012423 maintenance Methods 0.000 abstract description 2
- 239000000843 powder Substances 0.000 description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 206010003445 Ascites Diseases 0.000 description 2
- 241000209128 Bambusa Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 208000006268 Sarcoma 180 Diseases 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 235000021590 normal diet Nutrition 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
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- 241000894007 species Species 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007515 enzymatic degradation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
【課題】 ヒアルロン酸とペプタイドの二物質を含有するヒアルロン酸吸収用食品は、これまでの研究により優れた抗腫瘍効果を発揮することが判明している。本発明はそのようなヒアルロン酸吸収用食品に存する抗腫瘍効果をさらに強化するべくなされたものであり、その目的は健康維持機能に優れた健康食品を提供し、併せて優れた抗腫瘍作用を発揮する経口用抗腫瘍剤を提供することにある。
【解決手段】 ヒアルロン酸とペプタイドとアルコール溶液による竹抽出物の少なくとも三物質を含有してなる健康食品を提供する。この健康食品を経口摂取することにより、ヒアルロン酸とペプタイドの二物質を含有する健康食品に存する抗腫瘍効果を上回る抗腫瘍効果を発揮する。
【選択図】 図１
PROBLEM TO BE SOLVED: It has been found that hyaluronic acid-absorbing food containing two substances of hyaluronic acid and a peptide exhibits an excellent antitumor effect by previous studies. The present invention was made to further enhance the antitumor effect existing in such hyaluronic acid-absorbing food, and its purpose is to provide a health food excellent in health maintenance function, and also to have an excellent antitumor action. An object of the present invention is to provide an oral antitumor agent.
A health food comprising at least three substances of a bamboo extract made of hyaluronic acid, a peptide, and an alcohol solution is provided. By orally ingesting this health food, it exhibits an antitumor effect that exceeds the antitumor effect existing in health foods containing two substances, hyaluronic acid and peptide.
[Selection] Figure 1
Description
本発明は、人の健康を維持するための健康食品及び抗腫瘍作用を発揮する経口用抗腫瘍剤に関する。 The present invention relates to a health food for maintaining human health and an oral antitumor agent exhibiting an antitumor action.
本発明者は、蛋白質分解酵素で酵素分解してペプタイト状にした蛋白質とヒアルロン酸を有するヒアルロン酸吸収用食品を開発し、既に特許を取得している（特許文献１参照。）。
また、ヒアルロン酸の体内吸収に関しては、上記特許文献１以外にも特許文献２，３などに示されている。
Further, in-vivo absorption of hyaluronic acid is shown in
上記のヒアルロン酸吸収用食品は、これまでの研究により優れた抗腫瘍効果を発揮することが判明している。本発明は、ヒアルロン酸吸収用食品に存する抗腫瘍効果をさらに強化するべくなされたものであり、その目的は健康維持機能に優れた健康食品を提供し、併せて優れた抗腫瘍作用を発揮する経口用抗腫瘍剤を提供することにある。 The above-mentioned hyaluronic acid absorbing food has been found to exhibit an excellent antitumor effect by previous studies. The present invention was made to further enhance the antitumor effect existing in hyaluronic acid-absorbing foods, the purpose of which is to provide a health food excellent in health maintenance function, and also exhibit excellent antitumor action It is to provide an oral antitumor agent.
請求項１に記載したように、ヒアルロン酸と、ペプタイドと、アルコール濃度が５０％〜７０％である含水アルコール溶液による竹抽出物の少なくとも三物質を含有してなる健康食品を提供する。なお、前記ペプタイドは蛋白質を加水分解して体内吸収性を高めた物質として把握される。
また、請求項２に記載したように、前記ヒアルロン酸とペプタイドは、鳥の鶏冠を低分子化して抽出したものである請求項１記載の健康食品を提供する。
また、請求項３に記載したように、前記竹抽出物は、アルコール濃度が６０％である含水アルコール溶液で抽出したものである請求項１又は２記載の健康食品を提供する。
また、請求項４に記載したように、請求項１乃至３の何れか一つに記載の健康食品構成々分を有効成分とする経口用抗腫瘍剤を提供する。
As described in claim 1, a health food comprising at least three substances of hyaluronic acid, a peptide, and a bamboo extract with a hydrous alcohol solution having an alcohol concentration of 50% to 70% is provided. In addition, the said peptide is grasped | ascertained as a substance which hydrolyzed the protein and improved the body absorbability.
In addition, as described in
Further, as described in claim 3, the bamboo extract provides the health food according to
In addition, as described in
本発明の健康食品又は経口用抗腫瘍剤によれば、ヒアルロン酸を体内の組織細胞に効果的に供給しつつ、ヒアルロン酸とペプタイドの二物質を含有する健康食品に存する抗腫瘍効果を上回る抗腫瘍効果が得られる。従って日常的に経口摂取することにより悪性腫瘍（癌）の進行を防ぐか或いは改善させ得る効果がある。 According to the health food or the oral antitumor agent of the present invention, while effectively supplying hyaluronic acid to the tissue cells in the body, the antitumor effect surpassing the antitumor effect existing in the health food containing two substances, hyaluronic acid and peptide. Tumor effect is obtained. Therefore, daily oral intake has the effect of preventing or improving the progression of malignant tumor (cancer).
なお、ヒアルロン酸とペプタイドの起源は特段の限定を要しないが、好ましくは鳥の鶏冠を低分子化して抽出するのがよい。鳥の鶏冠には豊富なヒアルロン酸と適度な蛋白質が含まれており、これを低分子化することでヒアルロン酸とペプタイドの理想的な組合せが簡単に得られる。 The origin of hyaluronic acid and peptide is not particularly limited, but it is preferable to extract the chicken crown from a low molecular weight. The bird's hen's crown contains abundant hyaluronic acid and moderate protein, and the ideal combination of hyaluronic acid and peptide can be easily obtained by reducing the molecular weight.
以下に本発明の健康食品又は経口用抗腫瘍剤の具体的な製造方法について説明する。 Below, the specific manufacturing method of the health food or oral antitumor agent of this invention is demonstrated.
［ヒアルロン酸とペプタイド］
先ず、ヒアルロン酸とペプタイドの混合物の製造方法について、鳥の鶏冠を原料とする場合について次のＡ〜Ｄの工程に基づき説明する。
Ａ．鳥の鶏冠は通常冷凍されているため先ずこれを解凍する。そして、解凍した鶏冠を、０．１％の塩素を溶かした精製水でよく洗浄する。次に、洗浄した鶏冠をミンチ機によってミンチにする。
Ｂ．ミンチを反応釜に入れ、ここに濃度１〜２％程度の苛性ソーダを加えて１５時間程度放置する。これによってミンチが十分ふやける。
Ｃ．ふやけたミンチに塩酸を加えてＰＨ７〜８に戻し、蛋白質分解酵素たるプロテアーゼを０．５％加え、約２．５時間程度酵素分解（加水分解）した後、５０メッシュのスクリーンで一次濾過をし、さらに、目詰まり防止のために濾過助剤としてセライトを１％加えて１０ミクロンの濾紙で二次濾過をする。
Ｄ．前記濾過した濾液に活性炭を０．５％加えてよく撹拌する。この活性炭によって脱臭・脱色された濾液をさらに１ミクロンの濾紙で濾過して活性炭を取り除き、フリーズドライで乾燥させて粉末状に粉砕する。
[Hyaluronic acid and peptides]
First, a method for producing a mixture of hyaluronic acid and a peptide will be described based on the following steps A to D in the case of using a chicken's crown as a raw material.
A. The chicken's crown is usually frozen and thawed first. Then, the thawed chicken cap is thoroughly washed with purified water in which 0.1% chlorine is dissolved. Next, the washed chicken crown is minced with a mincing machine.
B. Mince is put into a reaction kettle, caustic soda having a concentration of about 1 to 2% is added thereto, and the mixture is allowed to stand for about 15 hours. As a result, the mince is fully expanded.
C. Hydrochloric acid is added to the minced mince to bring it back to pH 7-8, 0.5% of protease, which is a proteolytic enzyme, is added for about 2.5 hours, and then subjected to primary filtration with a 50 mesh screen. Further, in order to prevent clogging, 1% of celite is added as a filter aid and secondary filtration is performed with a 10 micron filter paper.
D. Add 0.5% activated carbon to the filtered filtrate and stir well. The filtrate deodorized and decolored by the activated carbon is further filtered through a 1 micron filter paper to remove the activated carbon, dried by freeze drying and pulverized into a powder.
上記工程を経て出来た粉末は鳥の鶏冠を低分子化して抽出したものであり、鶏冠中に含まれる蛋白質が低分子化したペプタイドと、数万以下の分子量に低分子化したヒアルロン酸とを含んでいる。
なお、ヒアルロン酸とペプタイドの起源となる原料は、鳥の鶏冠以外に動物の眼球や大動脈等の内臓などでもよい。また、ヒアルロン酸とペプタイドは互いに異なる原料から製造したものであってもよい。また、加水分解の手段も特に限定する必要はなく、蛋白質分解酵素による酵素分解以外にもアルカリ加水分解などでもよい。
The powder produced through the above process is obtained by extracting a bird's chicken crown with a low molecular weight. Contains.
In addition, the raw material from which hyaluronic acid and peptides are derived may be an eyeball of an animal or a visceral organ such as an aorta other than a chicken's crown. Further, hyaluronic acid and peptide may be produced from different raw materials. Further, the hydrolysis means is not particularly limited, and alkaline hydrolysis or the like may be used in addition to the enzymatic degradation using a proteolytic enzyme.
［竹抽出物］
次にアルコール溶液による竹抽出物の製造方法について説明する。原料になる竹は、淡竹、真竹、孟宗竹、バンブータリ（原産地：インドネシア）等、その種類は特に限定されず、また、種々の竹を寄せ集めた雑竹でもよい。また、成長した竹はもちろん若芽である筍でもよく、さらにまた、使用する部位も、稈、根茎、葉のいずれでもよい。竹は、筍を除いて伐採後１年未満でなるべく新鮮なものがよく、筍の場合は収穫後速やかに処理するのがよい。但し、何れの場合も新鮮なうちに冷凍保存すれば、長期間経過後も使用可能である。
[Bamboo extract]
Next, the manufacturing method of the bamboo extract by an alcohol solution is demonstrated. The kind of bamboo used as a raw material is not particularly limited, such as pale bamboo, true bamboo, Munetake bamboo, and bamboo butterfly (origin of origin: Indonesia), and may be miscellaneous bamboo collected from various bamboos. In addition, the bamboo that grows may be a young bud, and the part to be used may be a bud, a rhizome, or a leaf. Bamboo should be as fresh as possible in less than one year after harvesting, except for straw. In the case of bamboo, it should be processed promptly after harvesting. However, in any case, if it is stored frozen while it is fresh, it can be used after a long period of time.
次に竹を水洗して不純物を取り除いた後、粉砕機で粉砕して最終的に１０ｍｍ径程度のスクリーンを通過する大きさにする。スクリーンのない粉砕機の場合は、長さが３ｍｍ〜５ｍｍの大きさになるようにすればよい。次に１Ｋｇの粉砕竹を１０Ｋｇの含水アルコール溶液（好ましくはアルコール濃度５０％〜７０％、最も好ましくは６０％）に浸けて十分に色が出るまで待つ。そのための期間は常温で約六ヶ月である。また、６０℃程度に加熱した場合は約２時間でよい。 Next, the bamboo is washed with water to remove impurities, and then pulverized by a pulverizer so as to finally pass through a screen having a diameter of about 10 mm. In the case of a pulverizer without a screen, the length may be 3 mm to 5 mm. Then hydrous alcoholic solution of 10Kg crushed bamboo 1Kg (preferably an alcohol concentration of 50% to 70%, and most preferably 60%) and waits until it exits is sufficiently color immersed in. The period for that is about six months at room temperature. Moreover, when it heats to about 60 degreeC, about 2 hours may be sufficient.
次に遠心分離機で固液を分離して竹のアルコール抽出物である液体を回収し、その液体を６０℃に加熱しながら２０％の重量になるまで濃縮する。この濃縮した竹の抽出物に固形分等量の澱粉を加え、フリーズドライで乾燥させて粉末にする。 Next, the liquid is a bamboo alcohol extract by separating the solid and liquid with a centrifuge, and the liquid is concentrated to a weight of 20% while heating to 60 ° C. An equal amount of starch is added to the concentrated bamboo extract and dried by freeze drying to obtain a powder.
以上のようにして製造した竹抽出物の粉末に前記ヒアルロン酸とペプタイドを有する粉末を混合すれば、本発明の健康食品又は経口用抗腫瘍剤ができる。なお、健康食品又は経口用抗腫瘍剤の形態は、固形、粉末、顆粒、液体等どのようなものでもよく、また、前記粉末にビタミン等他の成分を加えるようにしてもよい。二つの粉末の混合割合は特に限定されないが、好ましくは１：１か又は竹抽出物をそれより少なくするのがよい。また、摂取量についても限定する必要はないが、大人が摂取する場合の大まかな目安としては、１日当たり２００ｍｇ以上が適当である。 The health food or oral antitumor agent of the present invention can be obtained by mixing the powder having the hyaluronic acid and the peptide with the bamboo extract powder produced as described above. The form of the health food or oral antitumor agent may be any form such as solid, powder, granule, liquid, and other ingredients such as vitamins may be added to the powder. The mixing ratio of the two powders is not particularly limited, but it is preferably 1: 1 or less bamboo extract. Moreover, although it is not necessary to limit also about intake, 200 mg or more per day is suitable as a rough standard when an adult takes.
［抗腫瘍実験１］
本発明の健康食品（又は経口用抗腫瘍剤）の抗腫瘍効果を確認するため、以下のような実験を行った。なお、ヒアルロン酸とペプタイドの抽出方法は上記のとおりであり（こうして製造した粉末を本実験において鶏冠抽出物という。）、また、アルコール溶液による竹抽出物は次のようにして製造した。
すなわち、筍の身（皮を剥いだ中味の部分で、通常食用にする部分）をエタノール濃度が６０％である含水エタノール溶液に浸けて６０℃×２時間加熱し、そうして抽出した液体を濾過し、減圧濃縮（５０℃）し、乾燥（６０℃）させて粉末にした。
以上の竹抽出物の粉末と前記鶏冠抽出物の粉末を１：１の割合で混合して本発明品を得た。そして、実験食として一般的な通常飼料と、その通常飼料に１％の本発明品を混ぜたものと、前記通常飼料に１％の鶏冠抽出物を混ぜたものと、前記通常飼料に１％の竹抽出物を混ぜたものを夫々用意した。
次に腹水癌細胞（sarcoma-180）１×１０６個を５週齢のICRマウス（雄性）に対して右鼠頚部皮下に移植し、この細胞移植当日より前記した四種の実験食を四群に分けたマウスに各群１種のみ自由摂取させ、隔週で腫瘍の大きさを計測し、腫瘍面積を算出した。
その実験結果を図１に示す。この実験結果から明らかなように本発明の健康食品（又は経口用抗腫瘍剤）は、何も混ぜない通常飼料はもちろん、鶏冠抽出物と竹抽出物を夫々単独で混合した飼料より優れた抗腫瘍作用を発揮することが確認できた。
なお、図１から明らかなようにアルコール溶液による竹抽出物単独でも抗腫瘍作用を発揮するのであるが、アルコール濃度を変えて抽出した竹抽出物単独、竹の種類を変えて抽出した竹抽出物単独、同種の竹でも稈の外周部と内周部で抽出した竹抽出物単独、熱水で抽出した竹抽出物単独で上記と同様の実験を行った結果、１００％濃度のアルコール溶液を使用するより水を含んだアルコール溶液を使用した方が強い抗腫瘍効果を発揮し、特にアルコール濃度が６０％である含水アルコール溶液を使用した場合に最も強い抗腫瘍効果を発揮することが確認された。この実験結果から鶏冠抽出物に混合する竹抽出物としては、含水アルコール溶液で抽出したもの、アルコール濃度が５０％〜７０％である含水アルコール溶液で抽出したもの、アルコール濃度が６０％である含水アルコール溶液で抽出したものがより好ましい。また、同様に竹の中でも、孟宗竹、筍の身、同種類の竹の場合は完成した稈の外周部が特に好ましい。ちなみに熱水で抽出した竹抽出物単独では有効な抗腫瘍作用が確認できなかった。
[Anti-tumor experiment 1]
In order to confirm the antitumor effect of the health food (or oral antitumor agent) of the present invention, the following experiment was conducted. In addition, the extraction method of hyaluronic acid and a peptide is as above (The powder manufactured in this way is called a chicken crown extract in this experiment), and the bamboo extract by an alcohol solution was manufactured as follows.
That is, the body of the cocoon (the part that has been peeled off and is the part that is usually edible) is immersed in an aqueous ethanol solution having an ethanol concentration of 60% and heated at 60 ° C. for 2 hours, and the extracted liquid is Filter, concentrate under reduced pressure (50 ° C.) and dry (60 ° C.) to a powder.
The bamboo extract powder and the chicken crown extract powder were mixed at a ratio of 1: 1 to obtain the product of the present invention. Then, a normal normal feed as an experimental food, a mixture of the normal feed with 1% of the product of the present invention, a mixture of the normal feed with 1% chicken crown extract, and 1% of the normal feed Each of the bamboo extracts was prepared.
Next, 1 × 10 6 ascites tumor cells (sarcoma-180) were transplanted subcutaneously into the right heel neck of 5-week-old ICR mice (male). The mice divided into groups were allowed to ingest only one species per group, the tumor size was measured every other week, and the tumor area was calculated.
The experimental results are shown in FIG. As is apparent from the experimental results, the health food (or oral antitumor agent) of the present invention is superior to a feed obtained by mixing a chicken crown extract and a bamboo extract alone, as well as a normal feed without mixing anything. It was confirmed that the tumor action was exerted.
As can be seen from FIG. 1, the bamboo extract alone with an alcohol solution exerts an anti-tumor effect, but the bamboo extract alone extracted by changing the alcohol concentration, the bamboo extract extracted by changing the kind of bamboo Independently, even with the same kind of bamboo, the same bamboo extract extracted from the outer and inner peripheries of bamboo shoots and the bamboo extract extracted with hot water alone were used. As a result, a 100% alcohol solution was used. It was confirmed that the use of an alcohol solution containing water exerted a strong antitumor effect, especially when a hydrous alcohol solution having an alcohol concentration of 60% was used. . From this experimental result, the bamboo extract to be mixed with the chicken crown extract is extracted with a hydroalcoholic solution, extracted with a hydroalcoholic solution having an alcohol concentration of 50% to 70%, or hydrous with an alcohol concentration of 60%. What extracted with the alcohol solution is more preferable. Similarly, among the bamboos, the outer periphery of the finished cocoon is particularly preferable in the case of a bamboo sword, a bamboo shoot, or the same kind of bamboo. Incidentally, an effective antitumor effect could not be confirmed with the bamboo extract alone extracted with hot water.
［抗腫瘍実験２］
実験食として一般的な日本クレア（株）製の通常飼料と、その通常飼料に前記実験１と同じ本発明品を１％混ぜたものと、前記通常飼料に１％の鶏冠抽出物を混ぜたものを用意した。
次に腹水癌細胞（sarcoma-180）１×１０６個を５週齢のICRマウス（雄性）に対して右下腹部皮下に移植し、この細胞移植当日より前記した三種の実験食を三群（一群当たり２０匹）に分けたマウスに各群１種のみ自由摂取させ、移植後２８日目に腫瘍を摘出しその重量を計測した。
その結果を図２に示す。この結果から明らかなように本発明の健康食品（又は経口用抗腫瘍剤）は、何も混ぜない通常飼料はもちろん、鶏冠抽出物のみを混合した飼料より優れた抗腫瘍作用を発揮することが確認できた。
[Anti-tumor experiment 2]
Normal diet made by Nippon Claire Co., Ltd., which is a general experimental diet, 1% of the same invention product as in Experiment 1 mixed with the normal diet, and 1% chicken crown extract mixed with the regular diet I prepared something.
Next, 1 × 10 6 ascites tumor cells (sarcoma-180) were transplanted subcutaneously into the right lower abdomen of 5-week-old ICR mice (male). Mice divided into (20 mice per group) were allowed to ingest only one species per group, and tumors were extracted 28 days after transplantation and their weights were measured.
The result is shown in FIG. As is clear from this result, the health food (or oral antitumor agent) of the present invention can exhibit an antitumor action superior to a feed containing only a chicken crown extract as well as a normal feed containing nothing. It could be confirmed.
以上本発明を実施の形態について説明したが、もちろん本発明は上記実施形態に限定されるものではない。例えば実施形態では竹抽出物を得るためのアルコール溶液としてエタノールを使用したが、メタノール等、その他のアルコール溶液であってもよい。また、実施形態ではアルコール濃度が６０％である含水アルコール溶液を使用したが、量産化する場合はアルコール濃度５０％〜７０％の含水アルコール溶液を使用してもよい。 The embodiments of the present invention have been described above, but the present invention is of course not limited to the above embodiments. For example, in the embodiment, ethanol is used as the alcohol solution for obtaining the bamboo extract, but other alcohol solutions such as methanol may be used. Moreover, although the hydrous alcohol solution whose alcohol concentration is 60% was used in the embodiment, a hydrous alcohol solution having an alcohol concentration of 50% to 70% may be used for mass production.
Claims (4)
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| US7972636B2 (en) | 2004-02-25 | 2011-07-05 | Hidemoto Kusaka | Beverage and medicament containing bamboo extract as a main ingredient |
| JP4576583B2 (en) | 2005-03-22 | 2010-11-10 | キユーピー株式会社 | Hyaluronic acid or a salt thereof, method for producing the same, and cosmetics and food compositions containing the same |
| WO2007099830A1 (en) | 2006-02-24 | 2007-09-07 | Q.P. Corporation | Novel low molecular weight hyaluronic acid and/or salt thereof, and cosmetic preparation, pharmaceutical composition and food composition each using same |
| JP4712746B2 (en) * | 2006-09-15 | 2011-06-29 | キユーピー株式会社 | Acidic beverage |
| JP5814496B2 (en) * | 2008-08-25 | 2015-11-17 | アダプトゲン製薬株式会社 | Antitumor agent for oral use |
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