JP3559482B2 - Lipase inhibitor - Google Patents

Description

【００１３】
【発明の効果】
このように、本発明によるリパーゼ阻害剤は、リパーゼ阻害活性がきわめて高い。またこの効果は、クローブのエタノール抽出物に顕著であった。 [0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a lipase inhibitor, and more specifically, it is possible to effectively inhibit pancreatic lipase involved in digestion and absorption of lipids in a living body to prevent and suppress obesity and hyperlipidemia, The present invention relates to a lipase inhibitor that can be used for preventing deterioration of foods and the like and odor, and for preventing and preventing diseases such as dermatitis caused by lipase.
[0002]
[Prior art]
In recent years, the diet of Japanese people has been increasing in calories, causing obesity which is a cause of lifestyle-related diseases such as hyperlipidemia, arteriosclerosis, and diabetes. Diet therapy, exercise therapy and the like have been proposed and implemented as treatment and prevention methods. In diet, fat is the highest calorie in food components, and its weight loss is considered to be effective in preventing obesity. However, dietary restrictions are excessively stressful and difficult to continue in daily life, and foods having a digestive absorption inhibitory effect have been developed.
[0003]
Fat in the diet is once broken down by pancreatic lipase and absorbed from the small intestine. For this reason, tetrahydrolipstatin (Biochem. J., 256, 357, 1988) has been developed as a drug that inhibits pancreatic lipase and excretes excess fat. There are many reports of side effects of this lipase inhibitor, and a lipase inhibitor with higher safety has been demanded, and application of the lipase inhibitory action contained in plants and the like has also been reported. For example, extracts of fruits and their seeds (Research Report, Faculty of Agriculture, Meiji University, 69, 16, 1985), soybean protein (Agric. Biol. Chem., 37, 1225, 1973), cereal protein (Nutrition Reports Int., 32, 1107, 1985), radish seed protein (Japanese Journal of Food Industry, 35, 430, 1988), herb (Biosci. Biotech. Biochem., 56, 1478, 1992), wheat (New Food Industry, 40, 65, 1997). , Seaweeds (Lipids, 34,441, 1999), components of oolong tea (JP-A-1-102022), peppers, pumpkin, shimeji, maitake, hijiki, green tea, black tea, water extract of oolong tea (JP-A-3-219872) , Grape seed , Oyster leaf, Puer tea, etc., 12 kinds of plant extracts (Japanese Patent Application Laid-Open No. 9-227398), Labiatae plants (Japanese Patent Application Laid-Open No. 10-262606), and tannins as condensed tannins (Br. J. Nutr., 60, 275, 1988) (Japanese Unexamined Patent Publication No. 9-291039), and the lipase inhibitory effects of specific tannin (Japanese Unexamined Patent Application Publication No. 8-259557) and epigallocatechin gallate (Japanese Unexamined Patent Application Publication No. 3-228664) contained in Kawataketsumei are reported.
[0004]
Further, as for crude drugs, 14 kinds of extracts such as peonies, oren, psyllium, peanuts, genoshoko, tea and the like (Japanese Patent Application Laid-Open No. 64-90131), 6 kinds such as Dokkatsu, Ryokyo, Amberjack, etc., against microbial lipases (JP-A-5-255100) is disclosed. In addition, hemicellulose and wheat bran (J. Food Sci., 49, 956, 1984) (Am. J. Clin. Nutr., 42, 629, 1985), phosphatidylcholine in soybean seeds (Meiji University Agricultural Science Research Report, 73, 9 , 1986), myo-inositol (J. Food Sci., 53, 250, 1988) and flavonoids include luteolin, apigenin, kaempferol, quercetin, myricetin (Japanese Patent Laid-Open No. 7-61927), and hesperidin (Biosci. Biochem., 61, 102, 1997). However, these prior arts do not describe the lipase inhibitory effect of the clove extract.
[0005]
[Problems to be solved by the invention]
INDUSTRIAL APPLICABILITY The present invention has a strong inhibitory effect on pancreatic lipase involved in digestion and absorption of fat in a living body, contributes to prevention and suppression of obesity and hyperlipidemia, and is used for prevention of deterioration of foods and the generation of bad smell It is intended to provide a highly safe lipase inhibitor and a food composition containing the same.
[0006]
[Means to solve the problem]
Natural spices are known for their various medicinal effects and have been used as foods for many years, so their safety is extremely high. From the extracts of these spices, those that inhibit pancreatic lipase were intensively searched. As a result, they have found that spices represented by cloves and extracts thereof have an excellent pancreatic lipase inhibitory action. That is, the present invention is a food composition comprising a lipase inhibitor containing an ethanol extract of clove as an active ingredient, and an ethanol extract of clove.
[0007]
BEST MODE FOR CARRYING OUT THE INVENTION
Cloves, for example, buds before flowering of Syzygium aromaticum have a strong aroma and are used as insecticides, analgesics, gastrointestinal drugs and the like.
Lemongrass, such as Cymbopogon citratus leaves, is used to treat abdominal pain, diarrhea, headache, fever and the like.
Cinnamon, eg, leaves of Cinnamomum verum, are used to treat nausea and hypertension.
Immature berries of allspice, such as Pimenta dioica, control chills.
[0008]
The plant of the present invention can use a dried product, a dried powder or the plant itself.
The extract of the present invention can be obtained, for example, by pulverizing, crushing, or cutting a plant or a dried product thereof and adding a polar or nonpolar solvent thereto. The extract of the present invention can be used as it is, but can be used alone or together with a suitable carrier in the form of a powder or paste by a method such as spray drying, freeze drying, reduced pressure drying, and fluid drying.
These plants and extracts have a high lipase inhibitory activity and can be used for suppressing and preventing obesity based on inhibition of digestion and absorption of fat, and can also be used for preventing degradation of foods and the like and odor generation due to lipase. .
[0009]
【Example】
Hereinafter, the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto.
[0010]
Example 1
The dried plants shown in Table 1 were pulverized, and a 10-fold amount of distilled water was added to the pulverized material, followed by heating at 100 ° C. for 1 hour. This was filtered after centrifugation to obtain an extract.
The clove powder was also subjected to ethanol extraction. A 10-fold amount of ethanol was added to the clove powder and left overnight. This was filtered, dried under reduced pressure, then redissolved in distilled water and used for the test.
[0011]
The lipase inhibitory activity was measured as follows.
Using dimercaprol tributyrate (BALB) as a substrate, 5-thio-2-nitrobenzoic acid produced by the reaction of the resulting dimethylcaprol with 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) Was determined by measuring the absorbance at 412 nm.
50 μl of extract, 50 μl of porcine pancreatic lipase (30 μg / ml, Sigma), 350 μl of 0.1 M Tris buffer (pH 8.5) containing 10 mg / ml DTNB, 3.48 m / ml phenylmethyl fluphonyl fluoride (PMSF) Was incubated at 30 ° C. for 5 minutes, 50 μl of an ethanol solution containing 6.69 mg / ml BALB and 5.73 mg / ml sodium lauryl sulfate was added, and the mixture was incubated at 30 ° C. for 30 minutes. 1 ml of acetone was added to stop the reaction, and the absorbance at 412 nm was measured. The results are shown in Table 1.
[0012]
[Table 1]

[0013]
【The invention's effect】
Thus, the lipase inhibitor according to the present invention has extremely high lipase inhibitory activity. This effect was remarkable in the ethanol extract of clove.

Claims (2)

A lipase inhibitor comprising an ethanol extract of clove as an active ingredient. A food composition comprising an ethanol extract of clove .