JP3548761B2 - Transparent thermostatic culture vessel for microscope observation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a transparent constant temperature incubator for microscopy capable of setting various culturing conditions by freely controlling temperature, concentration of carbon dioxide and, humidity under microscopic observation, thus enabling a continuous and simple observation and recording of the change with time in observing and photographing the culturing state of various cells in biological, genital or biotechnological research field. SOLUTION: This transparent constant temperature incubator for microscopy consists of a space capable of freely controlling the temperature by mounting transparent exothermic plates whose temperatures are automatically controlled to prescribed values by a temperature controller 1 on the top and the bottom, a carbon dioxide supply port 2 and an exhaust port 3 to control the carbon dioxide gas concentration in the space, an evaporating dish 5 to keep the humidity in the container made airtight by a sealing packing 4. As a result, the incubator can be set to various cell culturing conditions under microscopic observation.

Description

[0001]
[Industrial applications]
The present invention is a biological, reproductive or biotechnology research field, in the field of observation of the culture state of various cells, photographs, when recording such as video, while performing microscopic observation, temperature, carbon dioxide concentration, The present invention relates to a transparent constant-temperature culture vessel for microscopic observation, in which various conditions of culture can be set by freely controlling the humidity, so that the observation and recording of changes over time in the culture state can be performed continuously and easily.
[0002]
[Prior art]
When observing the culture state of cells in the biological, reproductive, or biotechnology research fields, or recording photographs, etc., temporarily remove the cells cultured in a separate large incubator from the incubator. And set it on a microscope for observation or photography. Alternatively, the entire microscope was enclosed in a plastic container to control the temperature, and a small additional container therein was used to control the carbon dioxide concentration and humidity, while performing microscopic observation or photographing.
[0003]
[Problems to be solved by the invention]
This had the following disadvantages.
(A) In the method in which cells cultured in a separate large-sized incubator are temporarily taken out of the incubator, it is not possible to observe the culture state of the cells over time or to record a video.
(B) The method of enclosing the entire microscope in a plastic container requires a large-scale device and requires a large installation space. Further, since the space for temperature control is large, temperature control in a portion where temperature control is originally required tends to be uncertain.
[0004]
[Means for Solving the Problems]
In order to achieve the above object, the transparent thermostatic culture container for microscopic observation of the present invention is a hermetically sealable container in which two transparent heating plates that can be automatically adjusted to a set temperature by a temperature controller are provided above and below, A carbon dioxide supply port and a discharge port for adjusting the concentration of carbon dioxide in the container, and an evaporating dish containing water for keeping the humidity in the container constant, while observing with a microscope, the temperature in the container, Various cells can be cultured while controlling carbon dioxide concentration and humidity. Preferably, it is sealed by a seal packing. The present invention will be described with reference numerals. By providing transparent heating plates (1) which can be automatically adjusted to a set temperature by a temperature controller on the upper and lower sides, a space capable of freely controlling the temperature is provided, and carbon dioxide in the space is provided. Providing a carbon dioxide supply port (2) for adjusting the concentration and a discharge port (3), and having an evaporating dish (5) for keeping the temperature in a container sealed with a sealing packing (4) Is a transparent constant-temperature culture vessel for a microscope that enables cell culture while controlling the temperature, carbon dioxide concentration, and humidity while observing with a microscope.
Preferably, a temperature measuring sensor is provided on each of the two transparent heating plates.
[0005]
[Action]
The temperature of the transparent heat generating plate (1) is automatically controlled at a temperature suitable for the cells to be cultured, an exhaust port is provided from the outlet (3) using a flow control valve (7), and carbon dioxide is supplied from the carbon dioxide supply port (2). Carbon is supplied in a timely manner. At that time, the water (8) in the evaporating dish (5) evaporates and the humidity in the container is kept constant. A container in which the time-dependent changes in the culture state of the cells can be continuously observed and recorded by focusing the microscope objective lens (6) in a state where the temperature, carbon dioxide concentration and humidity are stable.
[0006]
【Example】
Hereinafter, examples of the present invention will be described.
(A) In the transparent heating plate (1), electrodes (10) are fixed to both ends of a heating glass (9) provided with a transparent conductive film with a conductive adhesive, and lead wires (13) are provided.
(B) A temperature measurement sensor (12) is provided between the heat-generating glass (9) and the cover glass (11), and bonded with a transparent gel silicon adhesive. Although a thermocouple is used for the temperature measurement sensor (12), a thermistor or a resistance temperature detector may be used.
(C) The transparent heating plate (1) with the above configuration is automatically controlled at the set temperature by the temperature controller.
(D) In order to control the concentration of carbon dioxide in the container, the outer periphery of the container is air-tight by a sealing packing (4) and a clamping hook (14). It has an outlet (3) and a flow control valve (7).
(E) The humidity in the container is such that the water (8) in the evaporating dish (5) evaporates and a humidity of 95% or more is constantly maintained.
When the present invention is used, the temperature of the transparent heating plate (1) is automatically controlled at a temperature suitable for the cells to be cultured, and the flow adjusting valve (7) is discharged from the outlet (3). ) To provide an exhaust port, and supply carbon dioxide gas from the carbon dioxide supply port (2) in a timely manner according to the concentration. At this time, if the carbon dioxide gas is supplied with moderate humidification and heating, the stability is further improved. Further, the water (8) in the evaporating dish (5) evaporates and the humidity in the container is kept constant. This is a container in which the cell culture state can be observed and recorded with time by focusing the microscope objective lens (6) in a state where the temperature, carbon dioxide concentration and humidity are stable. At that time, depending on the microscope to be used, it is easy to use even if it is a type that is mounted on a microscope stage as shown in FIG.
[0007]
【The invention's effect】
According to the present invention, the following effects can be obtained in the field of research on biology, reproduction, and biotechnology when observing the culture state of various cells and recording photographs and the like.
(A) The cell culture can be performed over a long period of time while observing with a microscope, so that the time-dependent changes in the culture state can be observed under a microscope. You can record and leave.
(B) The whole is compact and does not take up installation space.
(C) Since the capacity of the whole container is small, the consumption of carbon dioxide gas is small and the running cost is low.
(D) If the two transparent heating plates are provided with temperature measuring sensors, the temperature can be controlled directly below and directly above the cells to be cultured, and accurate temperature control can be easily performed.
[Brief description of the drawings]
FIG. 1 is a perspective view showing an example of the present invention.
FIG. 2 is a sectional view (1) of the present invention.
FIG. 3 is a sectional view (2) of the present invention.
FIG. 4 is a sectional view and a circuit diagram of a heating plate (1).
FIG. 5 is a perspective view of a round type according to the present invention.
FIG. 6 is a sectional view of FIG . 5 ;
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Transparent heating plate 2 Carbon dioxide supply port 3 Outlet 4 Sealing packing 5 Evaporating dish 6 Microscope objective lens 7 Flow control valve 8 Water 9 Heating glass 10 Electrode 11 Cover glass 12 Temperature measurement sensor 13 Lead wire 14 Clamp hook 15 Microscope stage

Claims (3)

Two transparent heating plates that can be automatically adjusted to a set temperature by a temperature controller are installed vertically, a sealable container, and a carbon dioxide supply port and a discharge port for adjusting the carbon dioxide concentration in the container, Equipped with an evaporating dish containing water for keeping the humidity in the container constant, while observing with a microscope, temperature, carbon dioxide concentration, and various cells can be cultured while controlling the humidity in the container. Transparent thermostatic culture vessel for microscope observation. The transparent thermostatic culture vessel for microscopic observation according to claim 1, Two A transparent thermostatic culture vessel for microscopic observation, wherein a temperature measuring sensor is provided on each of the transparent heating plates. The transparent thermostatic culture vessel for microscopic observation according to claim 1 or 2, wherein the container is sealed with a seal packing.

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