JP3505260B2 - Micro-detection device for microbial observation and method for purifying contaminated groundwater vein using the same - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION This invention uses micro-sensing apparatus and that for new microorganisms, while observing the state of growth of soil microorganisms in the contaminated soil region present upstream of the contaminated groundwater vein, microorganisms breeding activities promptly and accurately performed the action necessary, remove contaminants, it relates contaminated underground water vein efficiency methods well purifying.

[0002]

2. Description of the Related Art In recent years, environmental pollution caused by production activities and other human activities has become a global problem. Examples of the environmental pollution include air pollution, water pollution, soil pollution, noise, vibration, ground subsidence, and odor. Damages caused by these environmental pollutions are roughly classified into health damages to human bodies and living environment damages. The health damage to the human body may be directly caused by water pollution or air pollution, or indirectly by ingesting marine products or agricultural products polluted by water pollution or soil pollution. On the other hand, living environment damage includes civil engineering / building damage due to ground subsidence, landscape / space occupation damage due to solid waste, unpleasant sensation damage due to noise, vibration, odor, etc., and animal / plant damage due to air pollution and water pollution. .
Of these, the one that has a great impact on life is the contamination of groundwater veins.

By the way, this groundwater vein pollution is caused by pollutants exuding from soil pollution existing in the upstream region, for example, organic compounds discharged from homes and factories or discarded, heavy metals, chemicals, or sprayed pesticides. It is the cause. Therefore, since the groundwater veins can be prevented from being polluted by purifying the soil pollution, various methods for purifying the polluted soil have been attempted so far. For example, if the pollutant is a volatile organohalogen compound such as trichloroethane or tetrachloroethylene, a method has been tried in which a gas is aerated to the soil, the pollutant is transported and removed, and then the aerated gas is treated to recover the pollutant. However, when the pollutant is a heavy metal, a method has been attempted in which the soil is solvent-extracted and the extracted solvent is contacted with a cation exchanger to collect the heavy metal. Since it requires a large amount of treatment agent and has not been put to practical use.

Due to the rapid progress of biotechnology in recent years, microorganisms that act on pollutants that have been considered to be difficult to decompose have been found, and researches for treating contaminated soil using these microorganisms have been conducted. It's coming.

By the way, in order to remove pollutants from contaminated soil by using microorganisms, soil samples are collected from various places in the contaminated area, microorganisms present therein are cultured, and the growth state is observed, thereby acting on the pollutants. Choose a good one,
The method of inoculating it to contaminated soil is adopted.
However, in such a method, a large number of samples have to be collected, and each of them has to be cultured to confirm the result. Therefore, the operation is complicated, and the effect is confirmed after inoculating the contaminated soil with microorganisms. It was also difficult to do.
However, until now, the growth status of such microorganisms has
Since there is no simple detector for observing
The method described above could not be performed efficiently.

[0006]

SUMMARY OF THE INVENTION The present invention is directed to a contaminated area.
Install in a boring hole drilled in the soil and
A simple microbial microphone that allows direct observation of the growth state of microorganisms
(B) A detector is provided for the purpose of providing a method for preventing contamination of a groundwater vein due to contaminated soil by using the detector .

[0007]

Means for Solving the Problems The present inventors have arranged in a plurality of borehole bored in contaminated areas located upstream of the underground water vein, observation of the peripheral wall of the hole directly microbial growth, breeding Seed to develop a device for detecting conditions
As a result of repeated studies, the present invention has been completed.

That is, according to the present invention, the lower end portion is a shaft coupling.
The mechanism allows it to bend freely, with a boring hole in the center
Support member for mounting and driving machine for vertical movement and rotation
And an objective arranged at the opening of the lower end of the endoscope.
Lens or solid-state image sensor and lighting lamp, open upper end of endoscope
Eyepiece, objective lens and eyepiece arranged in the mouth
The optical fiber bundle built into the endoscope
And between the wall of the borehole and the objective lens
Gap that works with the shaft coupling mechanism to control the distance
Microphone for observing microorganisms characterized by having an inter-sensor
(B) When purifying a contaminated groundwater vein and a polluted groundwater vein, bore holes are drilled at multiple locations in the contaminated soil area located upstream of the groundwater vein, and pollutants are used as nutrient sources through these boreholes. After inoculating the contaminated soil with microorganisms, attach the above-mentioned micro-detection device for microorganisms to each boring hole, detect the growth and reproduction state of microorganisms on the surrounding wall over time, and based on the obtained information, It is intended to provide a method for purifying a contaminated groundwater vein, which is characterized by appropriately controlling growth and reproduction conditions to reduce the pollutant and suppress the inflow of the pollutant into the groundwater vein.

[0009] Next, with reference to the accompanying drawings, illustrating the micro-detection device for microorganisms observation of the present invention. FIG. 1 is a vertical cross-sectional view of an example of a fiberscope type micro-detection device.
2 is a bottom view thereof, which is mounted in the boring hole 16 by a support member 6. The endoscope 4 constituting this device is vertically moved and rotated by the actuator 10 and the step motor 11 so that the growth and reproduction state of microorganisms can be observed uniformly along the wall surface of the boring hole 16. There is. The tip portion of the endoscope 4 is flexibly connected to the main body by a shaft coupling mechanism 5, and the gap sensor 2
The distance between the objective lens 1 and the wall surface is appropriately adjusted by the step motor 12 which is interlocked with the distance detected by. The wall surface facing the objective lens 1 is illuminated by the illumination lamp 3,
The condition of the observation surface reaches the eyepiece lens 14 from the objective lens 1 through the optical fiber bundle 8 and can be observed at any time from the upper end opening 15 of the endoscope through the naked eye or a microscope as necessary.
The light guide 9 connected to the illumination lamp 3 is connected to the light source 17 through the light guide connection port 13.

FIG. 3 is a longitudinal sectional view showing an example of a fixed image pickup device type micro-detecting device. Instead of the objective lens, a fixed image pickup device 1'having a camera function is provided at the lower end opening of the endoscope tube 4. It is arranged. The information on the wall surface obtained by the fixed image pickup device 1 ′ is input to the control unit 18 via the optical fiber bundle 8. If necessary, the personal computer can be connected to perform arithmetic processing and information processing, or can be connected to a television monitor to monitor the growth and breeding state of microorganisms on the wall surface of the bowling hole.

Next, the above-mentioned micro-detection device for observing microorganisms
Method for purifying contaminated groundwater veins
explain. In this method, first, a plurality of boring holes are formed in a contaminated region located upstream of a contaminated groundwater vein. FIG. 4 is a plan view showing the relationship between the contaminated water vein and the contaminated soil region and the location of the boring hole. That is, when the groundwater veins a and b are contaminated, the pollution source (I) must be present between them and the clean groundwater vein c upstream thereof. Around this pollution source (I), there is usually an area (II) containing contaminated soil contaminated by pollutants diffused from the pollution source. In the present invention, the pollution source (I) and the area (II) including the surrounding contaminated soil are collectively referred to as a contaminated soil area. The borehole is assumed to be located around the pollution source (I) and the area (II) containing the polluted soil with reference to the accumulated data, geological structure, surrounding topography, etc. Several places, usually 4-10
It is drilled at a place.

In the example of FIG. 4 , A, A
Around the area containing contaminated soil at four locations B, C, and D,
Boring holes are drilled at 6 positions b, c, d, e, and f, a total of 10 positions. The size of the boring hole is usually 50 to 200 mm and the depth is 5 to 20 m, but it can be made larger or deeper if necessary.

FIG. 5 shows a cross-sectional view of land in a region where a boring hole is formed. Below the pollution source (I), the groundwater vein (II
I) passes through, and is gradually polluted by pollutants leaching from the area (II) including the pollution source (I) and the surrounding contaminated soil. In the method of the present invention, next, a micro-detecting device for observing microorganisms is attached to each of the boring holes, and the wall surface of the boring holes is observed while vertically moving and rotating the micro-detecting device. Usually, in the contaminated soil, microorganisms whose nutrients are the pollutants are present, but their activity varies depending on the strain type and environmental conditions.

In the method of the present invention, the above-mentioned micro-detecting device for observing microorganisms is operated to observe the growth and reproduction state of the microorganisms on the surrounding wall surface, and if necessary, the active microorganisms are collected through the boring hole. Then, after inoculating the inactive soil, the information obtained by the observation is processed to appropriately control the growth and reproduction conditions of the microorganism. For this condition control, it is advantageous to use a computer to input information, perform arithmetic processing, and interlock the processing result with the supply of nutrient sources and the adjustment of environmental conditions.

[0015]

EXAMPLES Next, preferred embodiments of the present invention will be described with reference to examples.

First, the pollution source and the range of pollutant content are specified. Drilling holes at each point of the soil and determining the type and concentration of pollutants in the holes by the Kimitsu method, pollution groundwater sampling method, suction pump gas chromatography method, ATD / GC / PID analysis method, etc. ,
The pollution source and the pollution range are specified, and the pollution map shown in FIG. 4 is created. At this time, the type and number of microorganisms existing in the soil at each boring point and the surrounding environment of the existing microorganisms, such as temperature, humidity and pH, are also measured. These are measured for each soil stratum in the depth direction together with the type and concentration of the pollutant, and the distribution state in the depth direction is also grasped. If there is a groundwater vein, measure the type and concentration of pollutants contained in the groundwater.

In FIG. 4 , a region (I) surrounded by A, B, C and D is a pollution source, and a region surrounded by a, b, c, d, e and f contains a contaminated soil ( II). In addition, C indicates the upstream of the groundwater vein, and A and B indicate the downstream of the groundwater vein.

Next, based on the pollution map shown in FIG. 4 , pollution source inspection points (A, B, C and D), pollution range inspection points (a, b, c, d, e and f), groundwater vein inspection points. Boring (a, b, c), each hole is shown in Figure 1.
Install the micro-detector shown in. To understand the results of pollution control, it is necessary to reduce the water pollution level to below the standard value by inspecting each of the upstream and downstream water quality of the groundwater vein and implementing the soil remediation measures.

The inserted micro-detector is moved up and down and rotated to observe the growth and activity of microorganisms on the soil on the wall surface on a TV monitor, and if necessary, to reproduce the microorganisms and take necessary measures for the activity. Give. For this treatment, for example, when a microorganism is present in the soil, the nutrient source necessary for the microorganism is added, or a pH adjusting agent or a humidity adjusting agent is added to make it optimal for the reproduction and activity of the microorganism. Na p
Measures such as adjusting to H and humidity are performed. If necessary, a microorganism having a vigorous action may be inoculated and the same treatment may be performed. When the microorganism does not exist in the soil, the microorganism is newly added and the same treatment as described above is performed.

It is effective to record the growth and activity status of microorganisms by the micro-detection device and the degree of soil purification in time series, and to appropriately and swiftly perform the necessary measures for reproduction and activity of microorganisms that decompose pollutants. Is.

[0021]

According to the present invention, a simple microbe micro-detecting device is provided, and by using it , while observing the growth and activity of soil micro-organisms in a boring hole with precision,
Contaminants were polluted by reducing the pollutants from the polluted soil, suppressing the inflow of pollutants into the groundwater vein from the polluted soil, by rapidly and appropriately performing the measures to actively reproduce and activate the pollutant-degrading microorganisms. The groundwater vein can be efficiently purified.

[Brief description of drawings]

FIG. 1 is a fiberscope type microinspection system of the present invention .
Intelligent device vertical section

FIG. 2 is a bottom view of the above.

FIG. 3 is a fixed image pickup device type micro-detector of the present invention .
Longitudinal section

[Fig. 4] Contaminated water vein, contaminated soil area and boring hole
Plan view showing the drilling position

[Fig. 5] Land cross-section of the area where the boring hole is drilled

[Explanation of symbols]

1 Objective lens 1'fixed image sensor 2 Gap sensor 3 lighting lamps 4 Endoscope 5 shaft joint mechanism 6 Support members 8 optical fiber bundle 14 eyepiece 16 boring holes

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI G02B 23/26 (72) Inventor Norihisa Shigemori 2-8-11 Nishishinbashi, Minato-ku, Tokyo 7th Toyo Kaiji Building 8F Foundation Institute for Global Environmental Technology (72) Inventor Yuka Onozawa 2-8-11 Nishi-Shimbashi, Minato-ku, Tokyo 7th Toyo Kaiji Building 8th floor Institute for Global Environmental Technology (72) Inventor Hachiya Masahiko 2-8-11 Nishi-Shimbashi, Minato-ku, Tokyo 7th Toyo Kaiji Building 8th floor, Research Institute for Global Environmental Science and Technology (72) Inventor Kikei Suzuki 2-13-1 Kubo, Kimitsu-shi, Chiba Chiba Kimitsu City Hall, prefecture (56) References JP-A-4-254692 (JP, A) JP-A-3-132590 (JP, A) JP-A-2-157391 (JP, A) JP-A-2-101283 (JP , A) 1-308906 (JP, A) JP-A 1-210594 (JP, A) JP-A 7-57577 (JP, A) Special Table 4-501231 (JP, A) Special Table 7-508324 (JP, A) (58) Fields surveyed (Int.Cl. ⁷ , DB name) B09C 1/10 C02F 3/00 E21B 47/00 G02B 23/24

Claims (5)

(57) [Claims] 1. A support member (6) for attaching a lower end portion to a boring hole (16) in a central position in a freely bendable manner by a shaft coupling mechanism (5) , and a drive mechanism (10, 11) for vertical movement and rotation. Objective lens (1) or solid-state imaging device (1 ') and illumination lamp (3) arranged in an endoscope barrel (4) and an endoscope barrel lower end opening (7) , and an endoscope barrel upper end opening An eyepiece (14) arranged in (15) , an endoscope for connecting the objective lens (1) and the eyepiece (14)
The optical fiber bundle (8) built in (4) ,
A micro-sensor for observing microorganisms, characterized by further comprising a gap sensor (2) interlocking with the shaft coupling mechanism (5) for controlling the distance between the wall surface of the boring hole (16) and the objective lens. apparatus. 2. A microorganism observing micro detecting device according to claim 1, wherein in a fixed image pickup device instead of the objective lens. 3. In purifying a contaminated groundwater vein,
Drilled the borehole at a plurality of contaminated soil region located upstream of the underground water vein, claim 1 contaminants through the bore hole after the microorganisms to nutrient was inoculated into contaminated soil, each bore hole Or , the micro-detection device for observing microorganisms according to 2 is attached, and the growth and reproduction state of microorganisms on the surrounding wall surface are detected over time, and based on the obtained information,
A method for purifying a contaminated groundwater vein, which comprises controlling the growth and reproduction conditions of microorganisms to reduce the pollutants and suppress the inflow of the pollutants into the groundwater vein. 4. The purification method according to claim 3 , wherein the microorganism collected from a borehole in which the growth and reproduction of the microorganism is good is inoculated into the borehole in which the growth and reproduction of the microorganism is poor. 5. A contaminated soil through the borehole, according to claim 3 also supplies the nutrients for promoting the growth of microorganisms
Is the purification method described in 4 .