CN111644457B

CN111644457B - Bioremediation method for metal pollution treatment

Info

Publication number: CN111644457B
Application number: CN202010308770.8A
Authority: CN
Inventors: 钟辉
Original assignee: Liuzhou Geyou Environmental Protection Technology Co ltd
Current assignee: Liuzhou GeYou Environmental Protection Technology Co.,Ltd.
Priority date: 2020-04-18
Filing date: 2020-04-18
Publication date: 2021-10-29
Anticipated expiration: 2040-04-18
Also published as: CN111644457A

Abstract

The invention discloses a bioremediation method for metal pollution treatment, belonging to the technical field of soil remediation, and the bioremediation method for metal pollution treatment comprises the following steps: firstly, ploughing polluted soil, irrigating the soil, airing the soil until the soil is loose, injecting compound microorganism strains into the polluted soil, erecting an auxiliary treatment device, culturing microorganisms for 2-3 months, planting repair plants in a polluted repair area, paving an algae culture film in the auxiliary treatment device, culturing phototropic algae on the algae culture film, removing the whole plant after culturing for 10-12 months, realizing the isolation of the soil area to be treated from the outside by the auxiliary treatment device and forming a small greenhouse, and installing an exhaust pipe and a supply pipe on the auxiliary treatment device for supplementing nutrient substances and exhausting, wherein the algae culture film can timely fall off after propagation and expansion, so that the algae on the algae culture film can provide nutrients for the microorganisms and the plants.

Description

Bioremediation method for metal pollution treatment

Technical Field

The invention relates to the technical field of soil remediation, in particular to a bioremediation method for metal pollution treatment.

Background

In recent decades, due to global industrialization, a large number of potentially toxic substances have been discharged to biospheres, including heavy metals, which may enter the soil, rivers, groundwater through various routes. Heavy metals are pollutants that are difficult to control, have long incubation periods, are a chronic accumulation of hazards, are not easily perceived by people, and are not degradable, and once in the environment will produce a persistent series of pollution that is not easily removed. The tailings pond in the metal mine is an important source of heavy metal pollution in an environmental system, the tailings are solid wastes generated in the mining process of the mine, the wastes contain toxic heavy metals with high concentration, and after the toxic heavy metals are transported to the ground surface from the ground, due to the change of physical and chemical conditions, the release and migration of heavy metal elements in the tailings can generate serious heavy metal pollution to the ecological environment of the ground surface such as the nearby soil. In addition, tailings, waste rocks, and the like in the process of mine development require large-area stacking sites, thereby causing large occupation of land and damage to the original ecosystem of the stacking sites, and causing deterioration of ecological environment. The heavy metal elements in the soil of the mining area are various, such As Cd, Pb, Zn, Cu, As, Bi, Sn, Mo, Se, Te, Ti, Be and the like, and the elements can cause great harm to the ecological environment if precipitated and fixed in the soil or circulated in the nature along other phase interfaces, and directly or indirectly harm human health through contact, food chain and other ways. Slight heavy metal poisoning can cause symptoms such as dizziness, nausea, cough, chest distress, inappetence, skin allergy, mental retardation and the like, and serious heavy metal poisoning can harm the life of people.

A series of researches on the heavy metal contaminated soil remediation technology are carried out at home and abroad, and compared with the traditional physical and chemical remediation technologies such as foreign soil, soil turning, soil leaching and the like, the bioremediation is recognized as an eco-friendly in-situ green remediation technology due to good effect, investment saving, low cost, easy management and operation, no secondary pollution and the like

However, in the existing bioremediation, microorganisms and plants are mostly used for treating soil, but no auxiliary culture measures are taken for the microorganisms and the plants during the soil remediation, so that the microorganisms and the plants grow and reproduce slowly or even die when being transplanted to the polluted soil, and the efficiency and the effect of the soil remediation are influenced.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the invention aims to provide a bioremediation method for metal pollution treatment, which can realize the treatment of polluted soil by culturing microorganisms and repairing plants, and an auxiliary treatment device is erected in the soil area to be treated, a light-transmitting film and a light-transmitting film are laid on the auxiliary treatment device to isolate the space above the soil area to be treated from the outside and form a small greenhouse, so that the influence of the external environment on the culture of the microorganisms and the repairing plants is reduced, and an exhaust pipe and a supply pipe are arranged on the auxiliary treatment device, so that harmful gas in the device can be timely extracted, and nutrient solution can be provided for the plants and the microorganisms through a spray head on a bracket.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

1. A bioremediation method for metal pollution treatment comprises a soil remediation method, wherein the remediation method comprises the following steps

S1, turning over a soil layer polluted by heavy metal by using a soil ploughing machine to a depth of 30-50 cm;

s2, irrigating the soil to be treated in the step S1 to enable the soil to be in a flooded state, keeping for 3-5 days, and airing until the soil is loose;

s3, uniformly mixing the composite microbial strains with soil with the surface layer thickness of 20-25cm of the soil to be treated, erecting an auxiliary treatment device on a treatment area, laying a light-transmitting film on the outer side of the auxiliary treatment device to seal the space in the auxiliary treatment device and reduce the influence of the external environment on the propagation of microorganisms, inserting an exhaust pipe and a supply pipe on the auxiliary treatment device, extracting harmful gas generated by the propagation of the microorganisms through the exhaust pipe, and providing nutrient solution for the propagation of the microorganisms through the supply pipe;

s4, after the microorganisms are cultured for 2-3 months, planting restoration plants in the soil to be treated, planting the restoration plants in a mixed manner, paving isolation films on the inner walls of the auxiliary treatment devices, planting phototactic algae on the inner layers of two isolation films, forming a small greenhouse environment in the auxiliary treatment devices, volatilizing a nutrient injected into the auxiliary treatment devices and absorbing the nutrient by the algae on the algae culture films, blocking a laser sensor when the algae on the algae culture films propagate and expand to the boundary, driving a film fixing mechanism by the laser sensor to loosen the algae culture films, enabling the algae culture films to fall to the soil to be treated, and providing nutrients for the microorganisms and the plants in the soil to be treated by the algae on the algae culture films;

and S5, after every two algae culture membranes are separated, covering the algae culture membranes on the inner walls of the other two PE membranes by technicians, and removing the whole plant after culturing and repairing the plant for 10-12 months.

Further, supplementary device of administering is including installing at subaerial annular base, the joint has first support and second support on the annular base, the top joint of first support has the draw-in groove, second support joint is on first support, all cut the jack on first support and the second support, it has the fixed tube to peg graft in the jack, the exhaust tube is pegged graft in the fixed tube, the cladding has the printing opacity membrane on first support and the second support, the border joint of printing opacity membrane is in annular base's bottom, threaded connection has a plurality of ground nails on the annular base, ground nail runs through annular base and printing opacity membrane and inserts to ground, the inner wall upper berth of first support and second support is equipped with interior barrier film, can realize providing the isolation environment for microorganism and restoration plant, form small-size greenhouse, guarantee microorganism and plant stable growth, convenient dismantlement installation.

Further, the printing opacity membrane includes the PO base film, has connected gradually adhesive layer and waterproof ventilative layer on the PO base film, and PO base film and waterproof ventilative layer all have good light transmissivity, guarantee that the printing opacity membrane has good light transmissivity, guarantee the daylighting of plant in the device.

Furthermore, the inner isolation membrane comprises a PE membrane, an algae culture membrane covers the inner wall of the PE membrane, phototactic algae are planted on the algae culture membrane, the algae culture membrane is a biodegradable biological membrane, and the phototactic algae planted on the algae culture membrane absorb the nutrients volatilized in the auxiliary treatment device and absorb carbon dioxide generated by microorganisms, so that the utilization rate of nutrient materials injected into the auxiliary treatment device is improved.

Further, the repair plant is at least one of Viola baoshanensis, Cedar and Chinese goldthread.

Further, the composite microbial strain comprises at least one of bacillus megaterium, bacillus mucilaginosus, serratia marcescens, pseudomonas aeruginosa and streptomyces clausii.

Furthermore, first support and second support are hollow titanium alloy support, all are connected with the shower nozzle on the inner wall of first support and second support, and charge-in pipeline is all installed to the upper end of first support and second support, and the supply line is pegged graft on charge-in pipeline, conveniently injects into the nutrient agent for supplementary improvement device through first support and second support.

Further, all be connected with laser sensor on the inner wall of first support and second support, the upper and lower both ends of first support and second support all are connected with laser sensor assorted membrane fixed establishment, membrane fixed establishment includes the slider, be connected with the couple on the slider, the couple inserts in the algae cultivation membrane, the spout to the matching with the slider has all been dug to the upper and lower both ends of first support and second support, the bottom fixedly connected with of spout and slider are to the micro-gap switch who matches, when the algae reproduction on the algae cultivation membrane expands too greatly and influences the plant daylighting, can in time make membrane fixed establishment loosen the algae cultivation membrane, make the algae cultivation membrane fall to treating soil department, make the algae on the algae cultivation membrane provide the nutrient for the microorganism and the plant in the soil of treating.

Further, the joint has the fixed network between first support and the second support, and the fixed network is made for stainless steel strip establishment, and the fixed network sets up to four, and is spacing to printing opacity membrane and interior barrier film through the fixed network, consolidates first support and second support through the fixed network.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

(1) this scheme is administered contaminated soil through cultivateing the microorganism and repairing the plant, and treat to administer the soil region and erect supplementary treatment device, through laying printing opacity membrane and printing opacity membrane on supplementary treatment device and make the top space of treating to administer the soil region with keep apart outward and form small-size greenhouse, reduce the influence of external environment to the microorganism and the cultivation of repairing the plant, install exhaust tube and supply line on the supplementary treatment device, can in time take out the interior harmful gas of device and the shower nozzle on the accessible support provide the nutrient solution for plant and microorganism.

(2) The algae culture membrane is laid in the auxiliary treatment device, phototactic algae are cultured on the algae culture membrane to absorb partial harmful gas generated by microorganisms, the phototactic algae absorb volatile nutrient, the laser sensor is covered after the algae are excessively bred, the membrane fixing mechanism is timely triggered after the laser sensor is covered, the algae culture membrane is loosened, the algae culture membrane falls to the soil to be treated, the algae on the algae culture membrane provide nutrients for the microorganisms and plants in the soil to be treated, the algae culture is only carried out on two algae culture membranes at each time, and the influence of the algae breeding on plant lighting is prevented.

(3) The first support of this scheme and second support are hollow titanium alloy support, and the shower nozzle is installed to the ground step of two supports, and charge-in pipeline is all installed to the upper end of first support and second support, and the supply tube is pegged graft on charge-in pipeline, makes the supply tube pour into the nutrient agent into for waiting to pollute plant and microorganism in the soil through the shower nozzle on two supports, and the printing opacity membrane all has good light transmissivity with interior barrier film, and the restoration plant of planting in the messenger's pollution area region has good illumination regulation and nutritive environment.

Drawings

FIG. 1 is a perspective view of an auxiliary remedial device according to the present invention;

FIG. 2 is an exploded view of the auxiliary abatement device of the present invention;

FIG. 3 is a cross-sectional view of an auxiliary abatement device of the present invention;

FIG. 4 is a schematic view of the structure at A in FIG. 3;

FIG. 5 is a schematic view of the structure at B in FIG. 4;

FIG. 6 is a transverse cross-sectional view of the auxiliary abatement device of the present invention;

FIG. 7 is a flow chart of the present invention.

The device comprises an annular base 1, a first support 2, a second support 3, a fixing tube 4, a light transmission film 5, a PO base film 501, an adhesive layer 502, a waterproof and breathable layer 503, a fixing net 6, an inner isolating film 7, a PE film 701, an algae culture film 702, a sliding block 8, a hook 9 and a microswitch 10.

Detailed Description

The drawings in the embodiments of the invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1: referring to FIGS. 1 and 7, a bioremediation method for metal pollution remediation includes a soil remediation method of remediating metal pollution by using

s3, uniformly mixing a compound microorganism strain with soil with the surface layer thickness of 20-25cm of the soil to be treated, wherein the compound microorganism strain comprises at least one of bacillus megatherium, bacillus mucilaginosus, serratia marcescens, pseudomonas aeruginosa and streptomyces clausii, erecting an auxiliary treatment device on a treatment area, laying a light-transmitting film 5 outside the auxiliary treatment device to seal the space in the auxiliary treatment device and reduce the influence of the external environment on the propagation of microorganisms, inserting an exhaust pipe and a supply pipe on the auxiliary treatment device, pumping harmful gas generated by the propagation of the microorganisms through the exhaust pipe, and providing nutrient solution for the propagation of the microorganisms through the supply pipe;

s4, after the microorganisms are cultured for 2-3 months, planting restoration plants in the soil to be treated, wherein the restoration plants are mixed and planted, the restoration plants are at least one of viola baoshanensis and common snowball, meanwhile, the inner wall of the auxiliary treatment device is paved with the isolation films 7, phototactic algae are planted on the inner layers of the two isolation films 7, so that a small greenhouse environment is formed in the auxiliary treatment device, the nutrient injected into the auxiliary treatment device can be absorbed by the algae on the algae culture film 702 after being volatilized, when the algae on the algae culture film 702 is propagated and expanded to the boundary, the laser sensor is shielded, the laser sensor driving film fixing mechanism loosens the algae culture film 702, the algae culture film 702 falls to the soil to be treated, and the algae on the algae culture film 702 provide nutrients for the microorganisms and the plants in the soil to be treated;

s5, after every two algae culture membranes 702 are separated, technicians cover the algae culture membranes 702 on the inner walls of the other two PE membranes 701, and the whole plants are removed after the plants are cultured and repaired for 10-12 months.

Referring to fig. 1-3, the auxiliary treatment device comprises an annular base 1 installed on the ground, a first support 2 and a second support 3 are clamped on the annular base 1, a clamping groove is clamped at the top end of the first support 2, the second support 3 is clamped on the first support 2, jacks are drilled on the first support 2 and the second support 3, fixing pipes 4 are inserted in the jacks, an exhaust pipe is inserted in the fixing pipes, a light-transmitting film 5 is coated on the first support 2 and the second support 3, the boundary of the light-transmitting film 5 is clamped at the bottom end of the annular base 1, a plurality of ground nails are connected on the annular base 1 in a threaded manner, the ground nails penetrate through the annular base 1 and the light-transmitting film 5 to be inserted into the ground, inner isolation films 7 are laid on the inner walls of the first support 2 and the second support 3, a fixing net 6 is clamped between the first support 2 and the second support 3, the fixing net 6 is made of stainless steel bars, the four fixing nets 6 are woven, the light-transmitting film 5 and the inner separation film 7 are limited through the fixing net 6, and the first support 2 and the second support 3 are reinforced through the fixing net 6.

Referring to fig. 1-3, the light-transmitting film 5 includes a PO base film 501, an adhesive layer 502 and a waterproof breathable layer 503 are sequentially connected to the PO base film 501, and both the PO base film 501 and the waterproof breathable layer 503 have good light transmittance, so as to ensure that the light-transmitting film 5 has good light transmittance and light collection of plants in the device.

Referring to fig. 5-6, the inner isolation film 7 includes a PE film 701, an algae cultivation film 702 covers the inner wall of the PE film 701, phototactic algae is planted on the algae cultivation film 702, the algae cultivation film 702 is a biodegradable bio-film, and the phototactic algae planted on the algae cultivation film 702 absorbs the nutrients volatilized in the auxiliary treatment device and absorbs carbon dioxide generated by microorganisms, so as to improve the utilization rate of the nutrients injected into the auxiliary treatment device.

Referring to fig. 2-3, the first support 2 and the second support 3 are both hollow titanium alloy supports, the inner walls of the first support 2 and the second support 3 are both connected with spray heads, the upper ends of the first support 2 and the second support 3 are both provided with feeding pipes, and the supply pipes are inserted into the feeding pipes, so that the nutrients can be conveniently injected into the auxiliary treatment device through the first support 2 and the second support 3.

This scheme is administered contaminated soil through cultivateing the microorganism and repairing the plant, and treat to administer the soil region and erect supplementary treatment device, through laying printing opacity membrane 5 and interior barrier film 7 on supplementary treatment device and make the top space of treating administering the soil region with keep apart outward and form small-size greenhouse, reduce the influence of external environment to the cultivation of microorganism and repairing the plant, install exhaust tube and supply tube on the supplementary treatment device, the accessible installs air exhaust device additional on the exhaust tube and bleeds, be connected with the input that the nutrient solution merit device carried out the nutrient solution on the supply tube, harmful gas in the accessible time extraction device and the shower nozzle on the accessible support provide the nutrient solution for plant and microorganism.

Referring to fig. 3-4, the inner walls of the first bracket 2 and the second bracket 3 are both connected with laser sensors, the laser sensors are installed on the inner side walls of the brackets, and the laser sensor is positioned at the boundary of the inner isolation film 7, the upper and lower ends of the first bracket 2 and the second bracket 3 are both connected with a film fixing mechanism matched with the laser sensor, the film fixing mechanism comprises a slide block 8, the slide block 8 is connected with a hook 9, the hook 9 is inserted into the algae culture film 702, the upper and lower ends of the first bracket 2 and the second bracket 3 are both provided with chutes matched with the slide block 8 in the direction of the slide block 8, the bottom end of the chute is fixedly connected with a microswitch 10 matched with the slide block 8 in the direction of the slide block 8, when the algae on the algae culture film 702 is propagated and expanded too much to affect the plant lighting, the membrane fixing mechanism can be timely loosened to enable the algae culture membrane 702 to fall to the soil to be treated, so that the algae on the algae culture membrane 702 can provide nutrients for microorganisms and plants in the soil to be treated.

The algae culture membrane 702 is laid in the auxiliary treatment device, phototactic algae is cultured on the algae culture membrane 702 to absorb partial harmful gas generated by microorganisms, the phototactic algae also absorbs volatile nutrient, the laser sensor is covered after the algae are excessively bred, the membrane fixing mechanism is timely triggered after the laser sensor is covered, the algae culture membrane 702 is loosened, the algae culture membrane 702 falls to the soil to be treated, the algae on the algae culture membrane 702 provides nutrients for the microorganisms and plants in the soil to be treated, the algae culture is only carried out on two algae culture membranes 702 every time, and the influence of the algae breeding on plant lighting is prevented.

The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.

Claims

1. A bioremediation method for metal pollution remediation, comprising a soil remediation method, characterized in that: the repairing method comprises

s3, uniformly mixing the composite microbial strains with soil with the surface layer thickness of 20-25cm of the soil to be treated, erecting an auxiliary treatment device on a treatment area, laying a light-transmitting film (5) on the outer side of the auxiliary treatment device to seal the space in the auxiliary treatment device and reduce the influence of the external environment on the propagation of microorganisms, inserting an exhaust pipe and a supply pipe on the auxiliary treatment device, extracting harmful gas generated by the propagation of the microorganisms through the exhaust pipe, and providing nutrient solution for the propagation of the microorganisms through the supply pipe;

s4, after the microorganisms are cultured for 2-3 months, planting remediation plants in the soil to be treated, wherein the remediation plants are mixed and planted, meanwhile, the isolation films (7) are laid on the inner walls of the auxiliary treatment devices, phototactic algae are planted on the inner layers of the two isolation films (7), so that a small greenhouse environment is formed in the auxiliary treatment devices, the nutrients injected into the auxiliary treatment devices can be absorbed by the algae on the algae culture film (702) after being volatilized, when the algae on the algae culture film (702) propagate and expand to the boundary, the laser sensor is shielded, the laser sensor drives the film fixing mechanism to loosen the algae culture film (702), so that the algae culture film (702) falls to the soil to be treated, and the algae on the algae culture film (702) provides nutrients for the microorganisms and the plants in the soil to be treated;

s5, after every two algae culture membranes (702) are separated, technicians cover the algae culture membranes (702) on the inner walls of the other two PE membranes (701), and the whole plant is removed after the plants are cultured and repaired for 10-12 months;

the auxiliary treatment device comprises an annular base (1) arranged on the ground, a first bracket (2) and a second bracket (3) are clamped on the annular base (1), the top end of the first bracket (2) is clamped with a clamping groove, the second bracket (3) is clamped on the first bracket (2), jacks are drilled on the first bracket (2) and the second bracket (3), fixed pipes (4) are inserted in the jacks, the air exhaust pipe is inserted in the fixed pipe, the first support (2) and the second support (3) are coated with a light-transmitting film (5), the boundary of the light-transmitting film (5) is clamped at the bottom end of the annular base (1), the annular base (1) is in threaded connection with a plurality of ground nails which penetrate through the annular base (1) and the light-transmitting film (5) and are inserted into the ground, inner isolation membranes (7) are paved on the inner walls of the first support (2) and the second support (3); the inner isolation membrane (7) comprises a PE membrane (701), an algae culture membrane (702) covers the inner wall of the PE membrane (701), phototactic algae are planted on the algae culture membrane (702), and the algae culture membrane (702) is a naturally degradable biological membrane.

2. The bioremediation method for metal pollution abatement according to claim 1, wherein: the light-transmitting film (5) comprises a PO base film (501), an adhesive layer (502) and a waterproof breathable layer (503) are sequentially connected onto the PO base film (501), and the PO base film (501) and the waterproof breathable layer (503) both have good light-transmitting property.

3. The bioremediation method for metal pollution abatement according to claim 1, wherein: the repairing plant is at least one of Viola baoshanensis and herba Centellae.

4. The bioremediation method for metal pollution abatement according to claim 1, wherein: the compound microorganism strain comprises at least one of bacillus megaterium, bacillus mucilaginosus, serratia marcescens, pseudomonas aeruginosa and streptomyces clausii.

5. The bioremediation method for metal pollution abatement according to claim 1, wherein: the hollow titanium alloy support is characterized in that the first support (2) and the second support (3) are hollow titanium alloy supports, the inner walls of the first support (2) and the second support (3) are connected with spray heads, the upper ends of the first support (2) and the second support (3) are provided with feed pipes, and the supply pipes are inserted into the feed pipes.

6. The bioremediation method for metal contamination remediation according to claim 1 or 5, wherein: all be connected with laser sensor on the inner wall of first support (2) and second support (3), the upper and lower both ends of first support (2) and second support (3) all are connected with laser sensor assorted membrane fixed establishment, membrane fixed establishment includes slider (8), be connected with couple (9) on slider (8), couple (9) insert in alga cultivates membrane (702), the upper and lower both ends of first support (2) and second support (3) all open the spout to the matching with slider (8), the bottom fixedly connected with and slider (8) assorted micro-gap switch 10 of spout.

7. The bioremediation method for metal pollution abatement according to claim 1, wherein: the joint has fixed network (6) between first support (2) and second support (3), fixed network (6) are made for stainless steel strip establishment, fixed network (6) set up to four.