JP3441756B2 - Functional powdered beverage and method for producing the same - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a powdered beverage having an effect of multiplying intestinal useful bacteria and a method for producing the same. More specifically, improvement of human intestinal microflora, that is, by increasing the number of useful bacteria such as bifidobacteria and lactic acid bacteria in the intestine, a powdered beverage effective for promoting human health and a method for producing the same. .

[0002]

2. Description of the Related Art Conventionally, it has been difficult to observe bacteria in the intestine. However, due to remarkable progress in anaerobic culture technology in recent years, the number of bacteria in feces reflects the number of bacteria in the intestine, and many studies on intestinal bacterial flora have been conducted.
As a result, it has been clarified that the intestinal bacterium is closely related to human health and affects human physiological condition.

Further, these intestinal bacteria are involved in the metabolism of various substances derived from foods and drugs and endogenous substances, and are important for human nutrition, physiological functions, infectious immunity, carcinogenesis, aging, drug efficacy and the like. Playing an important role (Michioka Tomohi "The World of Enterobacteriaceae", Moubunsha, 1980; DJ Hentges
"Human Intestinal Microf
lora in Health and Diseases
e "Academic Press, 1983).

Among these intestinal bacteria, useful bacteria, such as bifidobacteria and lactic acid bacteria, which are advantageous in terms of defense against infection by human pathogens and viruses, nutrition, suppression of harmful bacterial growth, and Clostridium bacteria, There are harmful bacteria related to carcinogenesis, liver disease, arteriosclerosis, hypertension, infectious diseases, etc. such as pathogenic Escherichia coli. In addition, some harmful bacteria cause opportunistic infections and often bring about unfavorable situations such as sepsis, endocarditis, brain / liver / lung tumor, cystitis, vaginitis. (Michioka Chizu, "The story of intestinal bacteria", Iwanami Shoten, 1978.
Year).

From the above, it is considered that the optimum intestinal bacterial flora is to establish and grow useful bacteria in the human intestine. For this reason, various bifidobacteria preparations and yogurt have been developed in order to increase useful bacteria in the intestine, but gastric acid in the human body, passage through the upper gastrointestinal barrier such as bile acids, The establishment and growth ability of viable bacteria, such as the possibility of growth in the lower digestive tract as a growth site, is a serious problem.

On the other hand, the most important factor necessary for the growth of useful bacteria in the intestine is considered to be sugars, and various sugars such as lactulose, palatinose, fructooligosaccharides, raffinose, stachyose, and xylooligosaccharides are useful bacteria. Is said to be effective for the growth of
Although foods and drinks containing them have been proposed, these saccharides have assimilation to some harmful bacteria such as Escherichia coli and Clostridium bacteria, and are involved in their growth. Therefore, the saccharides are not selectively assimilated to useful bacteria, and the number of useful bacteria in the human intestine is not specifically increased under the present circumstances. From the above, satisfactory results have not yet been obtained.

[0007]

DISCLOSURE OF THE INVENTION An object of the present invention is to provide a powdered beverage capable of selectively growing only useful bacteria such as bifidobacteria and lactic acid bacteria among intestinal bacteria and a method for producing the same. . The useful bacteria referred to in the present invention refer to enterobacteria such as bifidobacteria and lactic acid bacteria that beneficially act on the health of the host, and harmful bacteria are intestinal bacteria such as Clostridium and pathogenic Escherichia coli that adversely affect the health of the host. Refers to inner bacteria.

[0008]

[Means for Solving the Problems] As a result of intensive studies conducted by the present inventors for the purpose of providing a powdered beverage that selectively grows useful bacteria in the human intestine, the mannose straight chain has a chain length of 30 to 30.
For the first time, it was found that by containing a galactomannan whose molecular weight is reduced to 80% or more in the range of 200 units, useful bacteria can be selectively grown and a very good powdered beverage per lot can be obtained. The invention was completed.

The low-molecular-weight galactomannan used in the present invention is, for example, guar gum, locust bean gum, tara gum, or carob gum, which is enzymatically mannose using β-mannanase derived from Aspergillus or Rhizopus. It can be obtained by hydrolyzing only a straight chain. The galactomannan can be changed in molecular weight by changing the reaction time of the enzyme, but the galactomannan added to the powdered beverage of the present invention is a mannose linear chain for the purpose of retaining the effect of growing intestinal useful bacteria. 80 within the range of chain length of 30 to 200 units
% Or more is preferable, and more preferably 50 to 1
The distribution is preferably within the range of 50 units. The chain length of the mannose linear chain of the present invention refers to the number of mannose that is the main chain of galactomannan bound, and the measuring method is not particularly limited, but for example, decomposed polysaccharides can be treated with water. Dissolved in water and using TOSO 803D type high performance liquid chromatography (HPLC) with water as the mobile phase.
Gel filtration is performed with a 3000 PW column and detection is performed with a suggestive refractometer. At this time, a linear dextrin having a known glucose number (glucose number 30, 100, 200) is measured as an index substance to obtain a graph as shown in FIG. From this, 30 to 200 units can be calculated from the area. If the chain length of mannose is shorter than 30 units, the content of reducing sugars will be high and the selective growth effect of only useful enteric bacteria cannot be expected. On the other hand, if the chain length of mannose is 200 units or more, Not only does it become viscous and has a very bad mouth feel when it is drunk, but it cannot be expected to have a proliferative effect on useful enteric bacteria due to its high molecular weight. Further, the dilution degree of the powdered beverage according to the present invention is such that 10 g of the powdered beverage has about one glass of water (18
0 to 200 cc) is desirable.

The galactomannan is preferably used in an amount of 0.08 to 0.83 g / kg of body weight per day, preferably 0.08, for obtaining an effective intestinal bacteria-proliferating effect.
If the dose is less than g / kg of body weight, the effect is weak.
If the amount is more than 3 g / kg body weight, unpleasant effects on humans such as abdominal satiety may occur.

A detailed description will be given below with reference to embodiments.

【Example】

Example 1 Citric acid was added to 900 parts of water to adjust the pH to 3.0. To this, 0.2 part of β-mannanase derived from Aspergillus and 100 parts of guar gum powder were added and mixed to obtain 40 parts.
The enzyme was allowed to act for 24 hours at ˜45 ° C. 90 ° C after reaction,
The enzyme was inactivated by heating for 15 minutes. The transparent solution obtained by separating the insoluble matters by filtration was concentrated under reduced pressure (solid content: 20%) and then spray-dried to obtain 65 parts of a white powder of low-molecular-weight galactomannan. The water-soluble dietary fiber content according to the enzyme gravimetric method was 80%. As a fixed bed, G3000PW (Tosoh Corp.) was used for the column.
As a result of measurement by high performance liquid chromatography using 80% or more of the sugar chains of the galactomannan, the chain length of mannose was within the range of 50 to 150 units. At this time, as a standard reagent of sugar chain unit, linear dextrin with known glucose number (glucose number 50, 10
0,150) was used.

Example 2 Using the galactomannan obtained in Example 1, an orange-taste fine powdery beverage was prepared at the compounding ratio shown in Table 1.

[0013]

[Table 1]

Test Example 1 Feces were collected twice during a control period in which 9 healthy adults were eating normally, and then Example 2 was used.
1 cup (180c) of 10g of the powdered beverage obtained by
Mix with water from c) and stir well, then take 12 each
It was ingested for a day, and feces were collected 4 times on the 5th, 7th, 11th and 13th days. Then, the ingestion of the drink was stopped, and feces were collected twice on the 13th and 15th days after the stop.

At the time of collecting feces 8 times in total, the number of intestinal bacteria in the feces, the detection rate and the occupation rate were determined. The number of bacteria and the detection rate are shown in Table 2, and the occupancy rate is shown in Table 3. It should be noted that Tables 2 and 3 show that the powdered beverage of the present invention is before drinking, during drinking-1 (5th and 7th days after the start of drinking), during drinking-
2 (11th day and 13th day of drinking), the results are shown divided into 4 periods after drinking. Also, 2 in each period
Since the feces were collected each time, the numerical value shows the average of 18 in total.

[0016]

[Table 2]

As shown in Table 2, by drinking the powdered beverage of the present invention, a significant increase in the number of Bifidobacterium, which is a useful bacterium among various intestinal bacteria, was observed, and the detection rate was a useful bacterium. A significant increase in lactic acid bacteria was observed respectively.

[0018]

[Table 3]

On the other hand, as shown in Table 3, a significant increase in the occupancy rate of the bifidobacteria, which is a useful bacterium, was observed by ingesting the product of the present invention.

As is clear from these, it was found that the product of the present invention has the effect of selectively growing useful enteric bacteria such as bifidobacteria and lactic acid bacteria.

Comparative Example 1 In the same manner as in Example 1, except that the reaction time was changed to 48 hours, galactomannan having a short mannose straight chain (80% or more of the chain length of mannose was within the range of 5 to 25 units). Was added to the powdered beverage) and a powdered beverage to which it was added was prepared in the same manner as in Example 2, and the same test was conducted on 9 healthy adults. The results are shown in Table 4 for the number of bacteria and the detection rate, and in Table 5 for the occupancy rate.

[0022]

[Table 4]

[0023]

[Table 5]

As is clear from Tables 4 and 5, even if the powdered beverage containing galactomannan having a shorter mannose chain length than that of Example 2 is ingested, no beneficial intestinal bacteria-proliferating effect is obtained. It was

[0025]

EFFECTS OF THE INVENTION The powdered beverage of the present invention has an extremely excellent proliferative effect on useful enteric bacteria such as bifidobacteria and lactic acid bacteria in the human intestine, and further has a very good mouthfeel and flavor. The invention contributes greatly to the promotion of human health.

[Brief description of drawings]

FIG. 1 is a diagram of an elution pattern of gel filtration detected by a suggestive refractometer.

Claims (2)

(57) [Claims] 1. A galactomannan having a low molecular weight such that the chain length of a mannose linear chain for producing a composition for growing useful enteric bacteria for a powdered beverage is 80% or more in the range of 30 to 200 units. how to use. 2. A composition for promoting the growth of intestinal useful bacteria for a powdered beverage, which comprises galactomannan having a low molecular weight such that the mannose straight chain has a chain length of 80% or more in the range of 30 to 200 units.