JP3438501B2 - Microorganism, method for decomposing acrylamide monomer, method for treating industrial wastewater, and method for decomposing acrylamide monomer in acrylamide-based polymer product - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel microorganism, a method for decomposing an acrylamide monomer using the microorganism, a method for treating industrial wastewater using the decomposing method, and a method for purifying an acrylamide polymer.

[0002]

2. Description of the Related Art In recent years, acrylamide polymers have been widely used as a treatment agent for wastewater treatment, a tertiary oil recovery agent for aged oil fields, a paper strength enhancer, a yield improver, and a coagulant in the papermaking field. , Its usage is increasing steadily. In particular, the acrylamide type paper strength enhancer used in the paper manufacturing industry has an effect of improving drainage and a yield in addition to the effect of increasing the paper strength, and thus is essential as an important chemical in the paper making process. The range has become even wider and its performance has been improved. Such an acrylamide polymer is synthesized by polymerization using an acrylamide monomer as a raw material, but unreacted acrylamide monomer remains inevitable in the acrylamide polymer obtained after its production. Acrylamide monomers are said to damage the nervous system of animals, and in order to prevent them from adversely affecting workers who manufacture and use related products of residual acrylamide polymers, acrylamide monomers are used. In the production of polymers, as a measure for reducing the amount of residual monomers, it has been attempted to lengthen the polymerization time or increase the amount of the polymerization initiator to increase the polymerization rate. Such measures complicate the production of the acrylamide polymer and increase the production cost, so that a more appropriate treatment method is desired. In particular, it is desired to develop a method for efficiently decomposing acrylamide monomers in industrial wastewater of acrylamide monomers, acrylamide polymer manufacturing plants, or plants that use acrylamide polymers.

As a countermeasure for this, a method of decomposing acrylamide monomer by a microorganism has been proposed. For example, Japanese Patent Laid-Open No. 52-79073 describes a method for decomposing acrylamide monomers using a strain of Brevibacterium ammoniagenes. In addition, JP-A-52
-87289 discloses a method for decomposing acrylamide monomers using a Bacillus strain, JP-A-52-9447.
No. 0 discloses a method for decomposing acrylamide monomer using a strain of Brevibacterium and a strain of Bacillus in combination,
Japanese Patent Application Laid-Open No. 52-94473 discloses a method for decomposing acrylamide monomers using a strain of the genus Brevibacterium and a strain of the genus Pseudomonas, and Japanese Patent Application Laid-Open No. 52-99281 discloses a strain of Bacillus and a strain of Pseudomonas. Japanese Patent Application Laid-Open No. 52-102489 describes a method for decomposing acrylamide monomers used in combination, and a method for decomposing acrylamide monomers in which strains of Brevibacterium, Bacillus and Pseudomonas are used in combination.

[0004]

However, these known methods for decomposing acrylamide monomers by microorganisms have been proposed as measures to improve the above-mentioned polymerization time and increase the polymerization rate by increasing the polymerization initiator. Then
It is difficult to efficiently decompose the acrylamide monomer remaining in the polyacrylamide polymer and the acrylamide monomer remaining in the industrial wastewater associated with the use of the acrylamide polymer.

The first object of the present invention is to provide a novel microorganism. A second object of the present invention is to provide a method for decomposing acrylamide monomers using a novel microorganism. A third object of the present invention is to provide a method for treating industrial wastewater by decomposing and removing acrylamide monomer in industrial wastewater, which is related to the use of acrylamide polymer using a novel microorganism. A fourth object of the present invention is to provide a method for decomposing acrylamide monomer in an acrylamide polymer product by decomposing and removing the acrylamide monomer remaining in the acrylamide polymer product containing the acrylamide polymer using a novel microorganism. It is in. A fifth object of the present invention is to realize the above-mentioned second to fourth objects with high efficiency, simplicity and low cost.

[0006]

Means for Solving the Problems The present inventors extensively searched for microorganisms capable of proliferating using acrylamide monomer as a carbon source and a nitrogen source, that is, microorganisms capable of degrading acrylamide monomer, and as a result, found that the soil of Okayama Prefecture Separated and identified Pseudomonas species No. 0351AM (Pseudomonas s
p. No. It was found that (0351 AM) has an excellent ability to decompose acrylamide monomers, and has completed the present invention. That is, in order to solve the above-mentioned problems, the present invention provides (1) Pseudomonas species No. 3 having the ability to decompose acrylamide monomers. 0351
AM (Pseudomonas sp. No. 0351
AM). The present invention also relates to (2), Pseudomonas species No. 0
351AM (Pseudomonas sp. No. 0
351 AM) to decompose the acrylamide monomer, (3), Pseudomonas species No. 0351AM (Pseu
domonas sp. No. 0351AM) for decomposing acrylamide monomer contained in industrial effluent using (4), acrylamide polymer product containing acrylamide polymer synthesized from acrylamide monomer containing Pseudomonas Species No. 0351A
M (Pseudomonas sp. No. 0351A)
A method for decomposing acrylamide monomers in an acrylamide polymer product, which comprises decomposing residual acrylamide monomers associated with the acrylamide polymer by using M), (5), acrylamide monomers in which the acrylamide polymer product contains acrylamide monomers. (4) consisting of an acrylamide polymer synthesized from
Method for decomposing acrylamide monomer in acrylamide polymer product, (6), The acrylamide polymer product according to (4), wherein the acrylamide polymer is a copolymer of acrylamide monomer and at least one other vinyl monomer. Method for decomposing acrylamide monomer in (7), wherein at least one of the other vinyl-based monomers is at least one of a cationic monomer having a cationic group and an anionic monomer having an anionic group. A method for degrading acrylamide monomers in polymer products is provided.

The present invention will be described in detail below. In the present invention, the acrylamide polymer means at least one kind of polymer synthesized from acrylamide monomers containing acrylamide monomer, and copolymer of acrylamide monomer and at least one other vinyl monomer. "At least one kind of other vinyl-based monomer" vinyl-based monomer includes other acrylamide monomers (a) other than acrylamide monomer,
A vinyl monomer (b) having a cationic group, a vinyl monomer (c) having an anionic group, a nonionic monomer (d), and a crosslinkable monomer (e) are included. Examples of the acrylamide monomers (a) include methacrylamide, N-methyl (meth) acrylamide, N-ethyl (meth) acrylamide, N, N-dimethyl (meth) acrylamide, N-isopropyl (meth) acrylamide, Nt. -N-substituted acrylamides such as octyl (meth) acrylamide are exemplified. One or two of these
One or more species can be used in combination.

Examples of the vinyl monomer (b) having a cationic group include dimethylaminoethyl (meth)
Acrylate, diethylaminoethyl (meth) acrylate, dimethylaminopropyl (meth) acrylate,
Tertiary amino groups such as diethylaminopropyl (meth) acrylate, dimethylaminopropyl (meth) acrylamide, diethylaminopropyl (meth) acrylamide, alkyldiallylamine, dialkyldiallylamine, diallylamine and allylamine, secondary amino groups, 1
Vinyl monomers having a primary amino group, salts of these inorganic or organic acids such as hydrochloric acid, sulfuric acid, formic acid and acetic acid, vinyl monomers having these tertiary amino groups and alkyl halides such as methyl chloride and methyl bromide, benzyl chloride , Benzyl bromide and other arylalkyl halides, dimethylsulfate, diethylsulfate, epichlorohydrin, 3-chloro-2-hydroxypropyltrimethylammonium chloride, glycidyltrialkylammonium chloride and other quaternizing agents. Vinyl monomer containing a secondary ammonium salt, 2-hydroxy N, N, N, N ', N'-pentamethyl-N'-[3-{(1-oxo-2-propenyl)
Amino} propyl] -1,3-propanediaminium dichloride and the like.

As the vinyl monomer (c) having an anionic group, a monovalent, divalent, trivalent, or tetravalent unsaturated carboxylic acid or a salt thereof such as acrylic acid, methacrylic acid, crotonic acid, etc. Monovalent unsaturated carboxylic acid,
Divalent unsaturated carboxylic acids such as maleic acid, fumaric acid, itaconic acid, muconic acid and citraconic acid, and organic sulfones such as vinylsulfonic acid, styrenesulfonic acid, allylsulfonic acid and 2-acrylamido-2-methylpropanesulfonic acid. Acids or sodium salts of these various organic acids,
Alkali metal salts such as potassium salts and ammonium salts,
3-butene-1,2,3-tricarboxylic acid, 4-pentene-1,2,4-tricarboxylic acid, aconitic acid, 4-
Pentene-1,2,3,4-tetracarboxylic acid or their sodium salts, potassium salts, ammonium salts,
Examples thereof include vinylphosphonic acid, isopropenylphosphonic acid, 1-butene-2-phosphonic acid, α-phenylvinylphosphonic acid or their sodium salts, alkali metal salts such as potassium salts, ammonium salts and the like.
The seeds may be used alone or in combination of two or more.

As the above nonionic monomer (d),
For example, esters of alcohols such as alcohols having 1 to 8 carbon atoms with acrylic acid or methacrylic acid, (meth)
Acrylonitrile, styrene, styrene derivatives, vinyl acetate, vinyl propionate, methyl vinyl ether, etc. are exemplified, and one of these can be used alone or in combination of two or more.

Examples of the crosslinkable monomer (e) include di (meth) acrylates such as ethylene glycol di (meth) acrylate, diethylene glycol di (meth) acrylate, triethylene glycol di (meth) acrylate and propylene glycol di (meth) acrylate. Acrylates,
Glycerin di (meth) acrylate, trimethylolpropane / ethylene oxide adduct triacrylate,
Methylenebis (meth) acrylamide, ethylenebis (meth) acrylamide, hexamethylenebis (meth)
Acrylamide, N, N'-bisacrylamide acetic acid,
Bis (meth) acrylamides such as N, N′-bisacrylamide methyl acetate, N, N-benzylidene bisacrylamide, etc., divinyl esters such as divinyl adipate, divinyl sebacate, epoxy acrylates, urethane acrylates, allyl (meth) ) Acrylate,
2 such as diallyl phthalate, diallyl malate, diallyl succinate, diallyl acrylamide, divinyl benzene, diisopropyl benzene, N, N-diallyl methacrylamide, N-methylol acrylamide, diallyl dimethyl ammonium, diallyl chlorendate, glycidyl (meth) acrylate Functional vinyl monomer, 1,3,5-triacryloylhexahydro-S-
Trifunctional vinyl monomers such as triazine, triallyl isocyanurate, N, N-diallyl acrylamide, triallylamine and triallyl trimellitate, tetramethylolmethane tetraacrylate, tetraallylpyromellitate, N, N, N ′, N '-Tetraallyl-1,
4-diaminobutane, tetraallylamine salt, tetrafunctional vinyl monomer such as tetraallyloxyethane, tetramethylolmethane-tri-β-aziridinylpropionate, trimethylolpropane-tri-β-aziridinylpropionate, Water-soluble aziridinyl compounds such as 4,4′-bis (ethyleneiminecarbonylamino) diphenylmethane, (poly) ethylene glycol diglycidyl ether, (poly) propylene glycol diglycidyl ether, (poly) glycerin diglycidyl ether, (poly) glycerin Water-soluble polyfunctional epoxy compounds such as triglycidyl ether, and 3- (meth) acryloxymethyltrimethoxysilane, 3- (meth) acryloxypropyldimethoxymethylsilane, 3- (meth)
Acryloxypropyltrimethoxysilane, 3- (meth) acryloxypropylmethyldichlorosilane, 3-
(Meth) acryloxyoctadecyltriacetoxysilane, 3- (meth) acryloxy-2,5-dimethylhexyldiacetoxymethylsilane, 3- (meth) acrylamidopropyltrimethoxysilane, 2- (meth) acrylamidoethyltrimethoxysilane, 1- (meth) acrylamidomethyltrimethoxysilane, 2- (meth)
Acrylamide-2-methylpropyltrimethoxysilane, 2- (meth) acrylamido-2-methylethyltrimethoxysilane, 2- (meth) acrylamidoisopropyltrimethoxysilane, 3- (meth) acrylamidopropyltriethoxysilane, N- ( 2- (meth) acrylamidoethyl) aminopropyltrimethoxysilane, (3- (meth) acrylamidopropyl) oxypropyltrimethoxysilane, 3- (N-methyl (meth))
Acrylamide) propyltrimethoxysilane, 3-
((Meth) acrylamide-methoxy) -3-hydroxypropyltrimethoxysilane, 3-((meth) acrylamide-methoxy) propyltrimethoxysilane, 3
-(Vinylbenzylaminopropyl) trimethoxysilane, dimethyl-3- (meth) acrylamide-propyl-3- (trimethoxysilyl) -propylammonium chloride, dimethyl-2- (meth) acrylamide-
2-methylpropyl-3- (trimethoxysilyl) -propylammonium chloride, 3- (meth) acrylamidopropylmethyldimethoxysilane, 3- (meth)
Acrylamidopropyldimethylmethoxysilane, 3-
(Meth) acrylamidopropylisobutyldimethoxysilane, 2- (meth) acrylamidopropylisobutyldimethoxysilane, 2- (meth) acrylamide-2
-Methylpropyl monochlorodimethoxysilane, 2-
(Meth) acrylamido-2-methylpropylhydrogendimethoxysilane, 3- (meth) acrylamidopropylbenzyldiethoxysilane, 3- (meth) acrylamidopropyltriacetoxysilane, 2- (meth) acrylamidoethyltriacetoxysilane, 4-
(Meth) acrylamidobutyltriacetoxysilane,
2- (meth) acrylamido-2-methylpropyltriacetoxysilane, N- (2- (meth) acrylamidoethyl) aminopropyltriacetoxysilane, 2-
(N-ethyl (meth) acrylamide) ethyltriacetoxysilane, 3- (meth) acrylamidopropyloctyldiacetoxysilane, 1- (meth) acrylamidomethylphenyldiacetoxysilane, 3- (meth) acrylamidopropyltripropionyloxysilane, Three
-(Meth) acrylamidopropyltri (N-methylaminoethoxy) silane, vinyltrichlorosilane, vinylmethyldichlorosilane, divinyldichlorosilane, vinylphenyldichlorosilane, vinyldimethylchlorosilane, vinylmethylphenylchlorosilane, vinyldiphenylchlorosilane, vinyltrimethoxy Silane, vinylmethyldimethoxysilane, vinylisobutyldimethoxysilane, vinyldimethylmethoxysilane, vinyltriethoxysilane, 3-vinylbenzylaminopropyltriethoxysilane, vinylmethyldiethoxysilane, divinyldiethoxysilane, vinyldimethylethoxysilane, vinyldiphenyl Ethoxysilane, vinyltriisopropoxysilane, vinyltributoxysilane, vinyldimethylisobuto Shishiran, vinyl triphenoxy silane, vinyl dimethyl (3-aminophenoxy) silane, vinyl dimethyl (4-aminophenoxy) silane,
Vinyl dimethyl (3-methyl-4-chlorophenoxy)
Examples thereof include silicon compounds such as silane, vinyldimethyl (2-methyl-4-chlorophenoxy) silane, vinyltriacetoxysilane, vinylmethyldiacetoxysilane, and vinyldimethylacetoxysilane.

In the present invention, the amount of each component (a) to (e) used is preferably in the following range. The amount of component (a) used is 100 to 70 mol%, preferably 100 to 80 mol%, based on the total molar amount of the monomer components used. Similarly, the component (b) is 20 mol% (2
0 mol% or less or 0 to 20 mol%, the meaning of "at most" is the same below), preferably at most 15 mol%,
Component (c) is at most 15 mol%, preferably at most 1
The amount of component (d) is at most 20 mol%, preferably at most 10 mol%. The component (e) is
It is in the range of at most 5 mol% with respect to the total molar amount of the used monomer components excluding the component (e). Note that (b) to (e)
Is zero for any one component, two components, three components and all components
May be These acrylamide polymers are polymers obtained by other known polymerization methods, and the molecular weight can be set arbitrarily, and the molecular weight is not particularly limited. The acrylamide polymer may be an aqueous solution, an emulsion, or a powder, but in the case of a powder, it is preferable to carefully dissolve it in water so as not to cause lumps (macomo shape).

In the present invention, Pseudomonas species No. 0351AM (Pseudomonas
sp. No. 0351AM), which can be widely collected from nature such as forests, uplands, and sludges. This Pseudomonas species No. 0351AM (Ps
eudomonas sp. No. 0351AM) is
It is a microorganism newly isolated and identified from the soil of Okayama prefecture by the present inventors. This microorganism was produced on November 19, 1996.
It has been deposited at the Institute of Biotechnology, Institute of Biotechnology, Institute of Industrial Science, dated to FERM P-15953.
The mycological properties are shown in Table 1. The mycological tests in these tables were carried out based on the test method in the second volume (revised edition) (1985) of "Classification and Identification of Microorganisms", edited by Takeharu Hasegawa.

[0014]

[Table 1]

The above-mentioned mycological properties are based on the Virginize Manual of Systematic Bacteriology (B
energy's Manual of System
tic Bacteriology) Vo. 1 (198
4 years), No. 0351AM is a gram-negative aerobic bacillus, which has motility due to polar flagella, and thus Pseudomonas
It is considered to be a bacterium belonging to the genus. Pseudomonas (Pseudomo) that have similar biochemical properties to this strain
Pseudomonas putida (Pse)
udomonas Putida) and Pseudomonas
Pseudomonas lemoig
nei). However, Pseudomonas Petida (P
Seudomonas putida) produces a fluorescent dye, whereas this strain does not produce a fluorescent dye. Pseudomonas remoignei (Pseudomo)
nas lemoignei) grows at 41 ° C, whereas this strain does not grow at 41 ° C, grows at less than 41 ° C, and particularly well at 20 to 37 ° C. Since it does not exist, it is considered that this strain is a new strain of the genus Pseudomonas. Therefore, this strain was designated as Pseudomonas species No. 0351AM (Pseudom
onas sp. No. 0351 AM). In the present invention, Pseudomonas species No. is not limited to the above strains or mutants thereof. 035
1AM (Pseudomonas sp. No. 035
1AM), and any bacterium that has the ability to decompose acrylamide monomers can be used.

As a medium composition for culturing the above-mentioned microorganisms, either natural medium or synthetic medium can be used, so long as these microorganisms can grow, and the medium may be either solid or liquid medium. For example, sugars such as glucose, fructose, and sucrose as carbon sources,
Organic acids such as acetic acid and citric acid, alcohols such as ethanol and glycerol, etc. as a nitrogen source, in addition to general natural nitrogen sources such as peptone, meat extract, yeast extract, protein hydrolysate and amino acid, various inorganic and organic ammonium salts Etc. can be used. In addition, inorganic salts such as potassium salt, magnesium salt, iron salt, calcium salt and the like are appropriately used as necessary. Culturing of the above-mentioned microorganism can be performed by a conventional method. Usually, the culture temperature is 20 to 3
It is performed at 7 ° C., preferably 20 to 35 ° C., pH 4 to 8.5, preferably pH 4.5 to 8.0 under aerobic conditions by means such as shaking or aeration stirring.

In the microbial decomposition of acrylamide monomer according to the present invention, the above microorganism is cultured in a medium containing acrylamide monomer as a carbon source and a nitrogen source and other inorganic salts, or the above microorganism is previously cultured as described above. After culturing under the conditions and collecting these microorganisms, the microorganisms may be contacted with an acrylamide monomer. It should be noted that the microorganism of the present invention can sufficiently decompose acrylamide monomers even in the presence of an acrylamide polymer. Further, known acrylamide monomer-degrading microorganisms include, for example, JP-A-52-
As described in Japanese Patent Publication No. 94473, microbial treatment of acrylamide monomer is carried out in a pH range of pH 6.5 to 8.5, and further diammonium hydrogen phosphate is added as a nitrogen source in a medium to add acrylamide monomer to single carbon. Processing as a source. On the other hand, since the microorganism of the present invention is capable of decomposing sufficient acrylamide monomer even in an acidic region, a polyacrylamide paper strength enhancer or the like in which the pH of the product containing the acrylamide polymer is 4-6. It is particularly useful for the above-mentioned paper additive for the decomposition of acrylamide monomer (residual monomer at the time of production or monomer produced at the time of natural decomposition due to long-term storage, etc.) which is incidental to the acrylamide polymer. Further, the microorganism of the present invention can utilize the acrylamide monomer as a single carbon source and a single nitrogen source, and therefore can efficiently decompose the acrylamide monomer. Further, in the present invention, in addition to the microorganism itself, an enzyme produced by the microorganism can be used. That is, a culture solution obtained by removing solids such as bacterial cells from a culture obtained by culturing a bacterium can also be used.

As the method for contacting the acrylamide monomer with the microorganism and / or enzyme used in the present invention, when the acrylamide monomer in the industrial wastewater related to the use of the acrylamide polymer is decomposed, the above-mentioned industrial wastewater is used. The microorganisms and / or enzymes described in 1 above may be added and mixed, and treated in the same manner as a general activated sludge treatment method. Further, in order to decompose the acrylamide monomer remaining in the acrylamide polymer obtained by the above-mentioned other known polymerization method or generated by the decomposition of the polymer, the above-mentioned microorganism and / or enzyme is added to the acrylamide polymer. Just mix. In general, the acrylamide polymer-based paper strengthening agent has a high product viscosity of 1000 cps or more (at least 1000 cps) by a Brookfield viscometer, and is therefore diluted before being used in a paper mill. However, the aforementioned microorganisms and / or enzymes may be added after dilution. When the microorganism of the present invention is brought into contact with the acrylamide polymer, the concentration of the aqueous solution of the acrylamide polymer is 30.
It is preferably less than or equal to wt%, preferably 0.001 to 5%. The contact time is usually 1 to 10 days, but the contact time depends on the amount of acrylamide monomer or the concentration of microorganisms in the system. For example, in the case of industrial wastewater containing 0.1% of acrylamide monomer, the contact time is 24 hours.
Can be decomposed by 00%. The decomposed products are water, carbon dioxide, ammonia and the like. Further, for example, in the case of an acrylamide polymer in which 0.08% of acrylamide monomer remains, about 80% of the remaining acrylamide monomer can be decomposed in a contact time of 24 hours. Also,
It is also possible to shorten the contact time by adjusting the initial addition amount of the microorganism.

In this way, the acrylamide monomer is utilized as a carbon source and a nitrogen source for microbial growth by the decomposition by the above microorganisms, and its decomposed products become water, carbon dioxide, ammonia and the like, which become substances existing in the natural world and are rendered harmless. And can be removed by volatilization. In addition, in the present invention, "Pseudomonas species
No. 0351AM (Pseudomonas sp.
No. "0351 AM)" means that not only the microorganism itself but also the above-mentioned enzymes produced by the microorganism, both of them, and at least one of them may be used together with other substances. It means that the cause of decomposability results from the microorganism. Further, "acrylamide-based polymer products containing acrylamide-based polymers" refers to paper strength additives, paper additives such as yield improvers, coagulants for water treatment, and other industrial products. Other ingredients may be added accordingly.

[0020]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Further,% in each example is% by weight. The medium used for the experiment was a medium sterilized by a conventional method. Reference Example 1 (Separation of Strain) About 2 g of a soil sample collected from various places in Okayama Prefecture is mixed with 10 ml of sterile physiological saline and left to stand. 100 μl of the supernatant was added to 10 ml of the medium shown in Table 2, and shake culture was performed at 30 ° C. for about 4 days. After the culture, 100 μl of the culture was newly inoculated into the above-prepared medium and shake culture was performed at 30 ° C. for about 3 days. The obtained culture was inoculated into a medium having the above medium composition with an acrylamide monomer concentration of 0.3%, and the culture was performed twice in the same medium. The culture was applied to a plate medium prepared by adding 1.5% agar to a medium having the above-mentioned medium composition with an acrylamide monomer concentration of 0.3%, and cultured at 30 ° C. for 7 days to form colonies. The formed colonies were purified twice, and Pseudomonas species No. 0351AM (Pseudomonas
sp. No. 0351 AM) was obtained. The microorganisms were identified according to the above test method, and the morphological, physiological and chemical properties of the isolated microorganisms were as shown in Table 1.

[0021]

[Table 2]

Reference Example 2 (Synthesis of anionic acrylamide polymer) A 50% acrylamide aqueous solution 24 was placed in a four-necked flask equipped with a stirrer, a thermometer, a reflux condenser and a nitrogen gas inlet tube.
Charge 3.3 g, acrylic acid 10.0 g, sodium methallyl sulfonate 0.51 g, water 614.97 g,
The pH was adjusted to 3.0, the temperature was raised to 60 ° C. under introduction of nitrogen gas, and then 8.44 g of a 5% ammonium persulfate aqueous solution was added,
The reaction was carried out at 0 ° C for 1 hour. The reaction product was cooled to room temperature to obtain an aqueous solution of an anionic acrylamide polymer having a solid content of 14.8%, a Brookfield viscometer viscosity (25 ° C.) of 6,850 cps and a pH of 4.3. When the amount of acrylamide monomer in this aqueous solution was measured by gas chromatography, the amount of acrylamide monomer was 0.08%.

Reference Example 3 (Synthesis of Amphoteric Acrylamide Polymer) In a four-necked flask equipped with a stirrer, a thermometer, a reflux condenser and a nitrogen gas inlet tube, a 50% acrylamide aqueous solution 52 was added.
9.0 g, dimethylaminoethyl methacrylate 31.
4 g, itaconic acid 10.4 g, 1% methylenebisacrylamide aqueous solution 21.6 g, isopropyl alcohol 3
6.0 g, water 1320.8 g, 20% sulfuric acid aqueous solution 48.
Charge 3 g to adjust the pH to 3.0 and introduce nitrogen gas at 60 ° C.
The temperature is then raised to 9.1, and then a 5% ammonium persulfate aqueous solution 9.1 is added.
2g and 9.52g of 2% sodium metabisulfite aqueous solution
Was added and reacted at 80 ° C. for 1 hour. While adding 86 g of water, the reaction was cooled to room temperature at a solid content of 15.6.
%, Viscosity by Brookfield viscometer (25 ° C) 1
An aqueous solution of an amphoteric acrylamide polymer having a pH of 4.9 and 2,000 cps was obtained. When the amount of acrylamide monomer in this aqueous solution was measured by gas chromatography, the amount of acrylamide monomer was 0.09%.

Example 1 Broth liquid medium (manufactured by Nissui Pharmaceutical Co., Ltd., medium pH 7)
Pseudomonas species No. 0351AM
(Pseudomonas sp, No. 0351A
One platinum loop of the cells of M) was inoculated and shake culture was performed at 30 ° C. for 3 days. 1 platinum loop of the obtained culture was inoculated into 10 ml of the medium (pH 6) of Table 2 shown in Reference Example 1 (using acrylamide mono as a single carbon source and a nitrogen source), and the growth of microorganisms Turbidity at 660 nm was measured by a spectrophotometer. The results are shown in Table 3.

[0025]

[Table 3]

Comparative Example 1 Known acrylamide momer-degrading bacteria A and B shown in Table 4
Tests were carried out in the same manner as in Example 1 except that was used to measure the growth of microorganisms. The results are shown in Table 3.

[0027]

[Table 4]

Example 2 Pseudomonas species No. was added to the medium (pH 4, 6, 8) of Table 2 shown in Reference Example 1. 0351AM (Pse
udomonas sp. No. One platinum loop was inoculated with the bacterial cells of 0351 AM), and shake culture was carried out at 30 ° C. for 3 days. 100 μl of the obtained culture was inoculated into 10 ml of the same medium newly prepared, and the degradation rate of acrylamide monomer and the growth of microorganisms were measured. The decomposition rate of acrylamide monomer is measured by gas chromatography GC-14.
A (manufactured by Shimadzu Corporation), using DB-WAX column (J &
(Manufactured by W Co.) and using FID as a detector to measure acrylamide monomer in the medium. For the growth of microorganisms, the turbidity of the culture at 660 nm was measured by a spectrophotometer. The results are shown in Table 5.

[0029]

[Table 5]

Example 3 In the medium used in Example 2, a test was conducted in the same manner as in Example 2 except that the acrylamide monomer concentration was 0.3% and the medium pH was 4.0, to decompose the acrylamide monomer. Rate and microbial growth were measured. The results are shown in Table 6.

[0031]

[Table 6]

Example 4 The aqueous solution of the anionic acrylamide polymer shown in Reference Example 2 was added to Pseudomonas species No. 03
51AM (Pseudomonas sp. No. 03)
51%) was added as a bacterial cell solid content in an amount of 0.1% and sufficiently stirred, and then the mixture was allowed to stand at 30 ° C. and the decomposition rate of acrylamide monomer was measured by gas chromatography. The results are shown in Table 7.

[0033]

[Table 7]

Example 5 The aqueous solution of the amphoteric acrylamide polymer shown in Reference Example 3 was added to Pseudomonas species No. 0351A
M (Pseudomonas sp, No. 0351A
M) was added as a cell solid content in an amount of 0.1% and sufficiently stirred, and the mixture was allowed to stand at 30 ° C. for 48 hours, and then the decomposition rate of acrylamide monomer was measured by gas chromatography.
The results are shown in Table 8.

From the results of the above Examples and Comparative Examples, Pseudomonas species No. 0351AM (Pseud
Omonas sp. No. 0351AM) is shown to utilize an acrylamide monomer as a carbon source and a nitrogen source, and it can be seen that the acrylamide monomer has a higher resolution than the bacteria A and B of Comparative Examples.

The above polymer solution can be used as it is or by adding other additives as a paper additive such as a paper strengthening agent, and the results similar to the above can be obtained for these products. Further, the results similar to the above can be obtained for industrial wastewater after using these products in the paper manufacturing method.

[0037]

According to the present invention, Pseudomonas species No. 0351AM (Ps
eudomonas sp. No. 0351AM), thereby providing a method for decomposing acrylamide monomers using this novel microorganism, a method for treating industrial wastewater that decomposes acrylamide monomers in related industrial wastewater using acrylamide polymers, and acrylamide polymers, etc. It is possible to provide a method for decomposing acrylamide monomer in an acrylamide polymer product, which can decompose and remove the acrylamide monomer remaining in the acrylamide polymer product, with high efficiency, simplicity and low cost.

[Table 8]

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI C08J 11/02 C08J 11/02 // (C12N 1/20 C12N 1/20 C12R 1:38) C12R 1:38 (56) Reference Documents JP-A-52-99281 (JP, A) JP-A-52-94473 (JP, A) JP-A-52-102489 (JP, A) JP-A-52-105273 (JP, A) JP-A-6- 134433 (JP, A) JP-A-51-144777 (JP, A) ARCH MICROBIOL (1990), Vol. 154, No. 2, p. 192-198 (58) Fields investigated (Int. Cl. ⁷ , DB name) C12N 1/00-1/38 C02F 3/00 C08J 11/00 BIOSIS / WPI (DIALOG)

Claims (7)

(57) [Claims] 1. Pseudomonas species No. having an ability to decompose acrylamide monomer. 0351AM
(Pseudomonas sp. No. 0351A
M) a microorganism. 2. Pseudomonas species No. 0
351AM (Pseudomonas sp. No. 0
351 AM) to decompose an acrylamide monomer. 3. Pseudomonas species No. 0
351AM (Pseudomonas sp. No. 0
351 AM) to decompose the acrylamide monomer contained in the industrial wastewater. 4. An acrylamide-based polymer product containing an acrylamide-based polymer synthesized from a monomer containing an acrylamide-based monomer, Pseudomonas species No. 0351AM (Pseudom
onas sp. No. A method for decomposing acrylamide monomer in an acrylamide polymer product, which comprises decomposing residual acrylamide monomer associated with the acrylamide polymer by using 0351AM. 5. The acrylamide polymer product comprises an acrylamide polymer synthesized from acrylamide monomers containing an acrylamide monomer.
The method for decomposing acrylamide monomer in the acrylamide polymer product according to 1. 6. The acrylamide polymer is at least one of an acrylamide monomer and another vinyl monomer.
The method for decomposing acrylamide monomer in an acrylamide polymer product according to claim 4, which is a copolymer with a seed. 7. The acrylamide monomer in the acrylamide polymer product according to claim 6, wherein at least one of the other vinyl monomers is at least one of a cationic monomer having a cationic group and an anionic monomer having an anionic group. Disassembly method.