JP3396934B2 - Denture cleaner - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a protease-containing denture cleanser having excellent stain cleaning power and stable against active oxygen.

[0002]

2. Description of the Related Art In recent years,
With the advent of an aging society, the number of denture users is increasing year by year. When using a denture, food waste tends to accumulate between the denture and the oral mucous membrane, and the inside of the mouth tends to become unclean compared to a healthy person. In particular, denture plaques of denture users have more Candida bacteria than dental plaques of healthy people, which is considered to be the cause of denture stomatitis.

The presence of denture plaques becomes a habitat for bacteria, causes unpleasant denture odors, and is a root cause of tartar formation, and forms stubborn stains that are difficult to remove with conventional foaming type denture cleansers. .

Other denture stains include food residues, stains (colored deposits), tartar and the like.
Of these, food residues can be easily removed by washing with water or brushing, and therefore their weight is low as a target for denture cleansing agents.

In the denture, not only the teeth but also the palate mucosa and the gingiva are made of resin or metal, so that the area to be soiled is large and the soiling is easily noticeable. In particular, the attachment of stains is not preferable from a hygienic and aesthetic point of view, and is a great target for denture cleansing agents.

Although the mechanism of formation of stain is not clear, it is said that the main cause is protein denaturation of pellicle composed of glycoprotein. There are two possible methods for removing stains with a denture cleanser. One is decomposition and bleaching of colored materials by active oxygen, and the other is decomposition of pellicle by enzymes.

From this point of view, it has been conventionally proposed to blend a denture cleanser with a protease as an enzyme (JP-A-48-58006 and JP-A-63-10131).
3, JP-A-1-125316, JP-A-4-3165.
However, there is a problem that protease is inactivated in the presence of active oxygen.

In this case, it is conventionally known that the enzyme is deactivated by active oxygen in this way, and therefore, for the purpose of preventing the deactivation of the enzyme, a two-layer tablet having an enzyme layer and an active oxygen layer is used. (JP-B-60-35325), various methods have been proposed, such as using an enzyme in combination with a specific protective agent (milk powder, gelatin, polyvinyl alcohol) (JP-B-63-25564). However, such methods limit and complicate formulation.

The present invention has been made in view of the above circumstances, and an object thereof is to provide a denture cleansing agent which is excellent in cleaning power for denture stains, stable to active oxygen, and easy to formulate.

[0010]

Means for Solving the Problems and Actions The inventors of the present invention have conducted extensive studies to achieve the above object, and as a result, they are derived from Bacillus lentus and have an optimum pH of 10 to 11 and an optimum pH. By using a protease having a temperature of 40 to 50 ° C., compared with the proteases that have been proposed and used in the conventional denture cleansing agents, the denture stain cleaning effect and the denture plaque removal effect are excellent, and the presence of active oxygen is present. It was found that the present invention is stable even under the following conditions, has little activity decrease, and effectively exhibits the excellent denture cleaning effect and denture plaque removing effect of this protease even after long-term storage. .

The present invention will be described in more detail below. The denture cleanser of the present invention contains Bacillus
From Lentus (Bacillus lentus),
It contains a protease having an optimum pH of 10 to 11 and an optimum temperature of 40 to 50 ° C.

As such a protease, for example, Durazyme (trade name) manufactured by Novo-Nordisk Co., Ltd. can be used.

The amount of the above-mentioned protease is 0.1 to 3
0% (% by weight, the same applies hereinafter), more preferably 0.1 to 2
0% is good. If it is less than 0.1%, a sufficient denture plaque removing effect may not be obtained. On the other hand, 30
%, But it is preferable to use a blending amount of 30% or less in view of the balance between economy and effect.

The denture cleanser of the present invention may contain other enzymes in addition to the above protease. Such enzymes include dextranase, mutanase, β-
1,3-D-glucanase and the like can be mentioned, and when these enzymes are used in combination, a more excellent denture plaque removing effect can be obtained. Particularly, by using the above-mentioned protease and dextranase in combination, a synergistically excellent denture is obtained. Shows the effect of plaque removal. The blending amount of such other enzyme is preferably 0.01 to 10%.

Further, it is preferable that an active oxygen generating substance is blended in the denture cleanser of the present invention. That is, as described above, since the protease according to the present invention has excellent resistance to active oxygen and excellent heat resistance, there is little inactivation during storage and washing, and the effect of active oxygen and the effect of the protease are effectively exhibited. Gives excellent cleaning effect.

Examples of the active oxygen generating substance include potassium monopersulfate (oxane), sodium perborate (perbon), sodium percarbonate and the like. One of these may be used alone or two or more of them may be used in combination. can do.
The blending amount thereof is preferably 10 to 80%, particularly preferably 20 to 70%. If it is less than 10%, sufficient bleaching power may not be obtained, and if it is more than 80%, the bubble strength may decrease.
Total balance causes quality deterioration.

Further, the denture cleanser of the present invention may contain one or more condensed phosphates such as sodium pyrophosphate, sodium tripolyphosphate, and sodium acid polyphosphate in order to stabilize the enzyme. It is preferable from the point. The amount of the condensed phosphate compounded is preferably 1 to 30%, particularly preferably 3 to 20%. If it is less than 1%, the combined effect with the enzyme is not observed, and if it exceeds 30%, the combined effect reaches saturation, and further addition is meaningless.

Furthermore, by blending the denture cleanser of the present invention with a carbonate such as sodium hydrogen carbonate, sodium carbonate or potassium carbonate and an acid agent such as anhydrous citric acid, succinic acid or malic acid, Foams when dissolved in water,
It is possible to promote the dissolution and dispersion of other components in water. Furthermore, it is preferable to add an anionic, nonionic or amphoteric surfactant in order to improve the detergency and ensure appropriate bubbles. Examples of such a surfactant include linear alkylbenzene sulfonate sodium and sodium lauryl sulfate.

The denture cleanser of the present invention is prepared in the form of powder, granules, tablets, etc., and components such as excipients suitable for such formulation can be added.

The denture cleanser of the present invention is used by dissolving it in water at the time of use, and the pH of the solution at the time of use is 5
In the range of from 11 to 11, good denture plaque removing power and stain removing power are exhibited, so that an appropriate pH adjusting agent can be blended.

[0021]

Industrial Applicability The denture cleanser of the present invention has an excellent denture cleansing effect and a denture plaque removing effect, and the protease is stable in the presence of active oxygen. It is not always necessary to devise a formulation, and the formulation is simple.

[0022]

EXAMPLES The present invention will be specifically described below by showing experimental examples and examples, but the present invention is not limited to the following examples. The proteases used in the following examples are as shown in Table 1.

[0023]

[Table 1]

[Experimental Example 1] Evaluation of denture stain (tannin stain) cleaning power Preparation of denture cleaning agent A powdery denture cleaning agent prepared by mixing enzymes with different excipients was prepared according to the formulation shown in Table 2 below. did.

[0025]

[Table 2]

Preparation of Tannin Stain Chips A white acrylic chip that had been sandblasted was prepared using
5% albumin solution → 3% Japanese tea + 1% coffee +1
% Aqueous solution of black tea → 0.57% aqueous solution of ammonium iron citrate, which is repeatedly dipped for 1 hour each, and this operation is continued for 2 weeks. The sample is taken out of the stain solution, lightly brushed the surface of the acrylic chip in running water to remove stains with weak adhesion, and then air-dried to obtain a sample chip. Evaluation method of tannin stain detergency The color difference (E2) of the sample chip to which tannin stain is attached is measured, and the sample chip is placed in a beaker filled with 150 ml of distilled water and kept at a constant temperature of 25 ° C. 3.0 g of the denture cleanser immediately after preparation of the above Formulations 1 to 8 is added, the denture cleanser is washed for 20 minutes, and the color difference (E3) of the washed sample chip is measured. The cleaning rate was calculated by the following formula, and the cleaning power was evaluated according to the following criteria. The results are shown in Table 3. Cleaning rate (%) = [(E3-E2) / (E1-E2)] × 100 However, E1 is the cleaning power of the color difference tannin stain of the chip before the tannin stain is attached 91% or more: ○ 70 to 90%: △ 69% or less: ×

[0027]

[Table 3]

[Experimental Example 2] Evaluation of protease activity after storage over time Storage conditions 50 g of the denture cleansing agent prepared in Experimental Example 1 was filled in a polypropylene container having an internal volume of 100 ml, and a hole of about 1 mm was formed in the center of the lid. Open it to make it an open system. It was stored in a constant temperature / humidity chamber at 40 ° C. and 75% for 1 month and then taken out to obtain a sample. Enzyme stability evaluation method 150 ml of distilled water is placed in a beaker and kept at a constant temperature of 40 ° C. Add 3.0 g of the above-mentioned prescription 1 to 7 denture cleaner,
The protease activity after time was measured. From the residual rate, the stability was evaluated according to the following criteria. The results are shown in Table 4. The activity of protease was measured by the method of Anson-Hagiwara et al. Enzyme residual rate 71% or more: ○ 50 to 70%: △ 49% or less: ×

[0029]

[Table 4]

[Experimental Example 3] Evaluation of removing power of denture plaque during cleaning Evaluation method of removing power of denture plaque Candida albicans and Str
50 μl of pre-cultured bacterial solution of ptococcus sobrinus (concentration of 0.1 at turbidity OD = 550 nm)
And BHI medium (37gB containing 1% sucrose solution)
HI + 10g sucrose / l concentration) 3ml
Was added to the test tube, and the test tube was kept at a 30 ° inclination.
Anaerobic culture is performed for 0 hours to prepare an artificial plaque. Wash twice with distilled water to remove artificial plaques that are not completely attached to the wall of the test tube.

Next, after adding 4 ml of distilled water, 100 mg of the denture cleaner prepared in Experimental Example 1 was added, and the mixture was kept at 37 ° C. for 3 days.
It was left to act for 0 minutes. Similarly, after washing twice with distilled water,
The remaining plaque after leaving for a while was dispersed by ultrasonic waves, and the turbidity (OD = 550 nm) was measured. A denture cleanser containing no enzyme was separately manufactured, and the same operation was performed (for OD control), and the removal rate of denture plaque was calculated according to the following formula. The results are shown in Table 5. Removal rate (%) = {(OD control-OD sample)
/ OD control} × 100 Denture plaque removing power was evaluated according to the following criteria. Denture plaque removing power 81% or more: ◎ 60 to 80%: ○ 30 to 59%: △ 29% or less: ×

[0032]

[Table 5]

From the above results, by using the protease according to the present invention, it has excellent denture stain cleaning power,
Moreover, it was found that it is stable in the denture cleanser in the presence of the active oxygen generating substance and has an excellent denture plaque removing power.

[Example 1] Potassium monopersulfate 12 Sodium perborate 8 Sodium carbonate Balance sodium hydrogen carbonate 35 Anhydrous citric acid 15 Lactose 5 Polyvinylpyrrolidone 3 Sodium tripolyphosphate 12 Sodium lauryl sulfate 1 Dextranase 1 Inventive protease 2 Powdered fragrance 1 Blue No. 1 Fine total 100%

Example 2 Potassium monopersulfate 10 Sodium perborate 10 Sodium percarbonate 45 Sodium carbonate balance Tripolyphosphate 10 Anhydrous sodium sulfate 5 Sodium lauryl sulfate 3 Dextranase 2 Protease 5 of the present invention 2 Perfume 2 Blue 1 No. 100%

[Example 3] Potassium monopersulfate 20 Sodium perborate 30 Sodium carbonate Balance sodium hydrogen carbonate 20 Anhydrous citric acid 10 Polyethylene glycol 3 Lactose 2 Polyvinylpyrrolidone 2 Sodium tripolyphosphate 3 Anhydrous sodium sulfate 5 Sodium lauryl sulfate 1 Invention Protease 1 Powdered fragrance 1 Blue No. 1 Fine total 100%

Example 4 Potassium monopersulfate 35 Sodium perborate 15 Sodium carbonate Balance sodium hydrogencarbonate 25 Anhydrous citric acid 10 Polyethylene glycol 1 Polyvinylpyrrolidone 1 Sodium tripolyphosphate 5 Sodium lauryl sulfate 1 Dextranase 0.5 Invention Protease 1 Powdered fragrance 0.5 Blue No. 1 Fine total 100%

Example 5 Potassium monopersulfate 15 Sodium perborate 5 Sodium percarbonate 10 Sodium carbonate balanced sodium hydrogencarbonate 20 Anhydrous citric acid 15 Polyethylene glycol 1 Lactose 5 Sodium tripolyphosphate 10 Sodium lauryl sulfate 0.5 Dextra Nase 0.5 Protease of the present invention 0.5 Powdered fragrance 1.5 Blue No. 1 Fine total 100%

Example 6 Potassium monopersulfate 5 Sodium percarbonate 60 Sodium carbonate Balance sodium tripolyphosphate 10 Anhydrous sodium sulfate 5 Sodium lauryl sulfate 2 Protease of the present invention 10 Powdered fragrance 1 Blue No. 1 Micrometer 100%

─────────────────────────────────────────────────── ─── Continuation of the front page (58) Fields surveyed (Int.Cl. ⁷ , DB name) A61K 7/30

Claims (3)

(57) [Claims] 1. An optimum pH derived from Bacillus lentus.
The denture cleansing agent is characterized by containing a protease having a pH of 10 to 11 and an optimum temperature of 40 to 50 ° C. 2. The denture cleanser according to claim 1, further comprising an active oxygen generating substance. 3. The denture cleanser according to claim 1, which further comprises dextranase.