JP3366776B2 - Serum neutral lipid metabolism or serum cholesterol metabolism improving food - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention improves the texture of foods containing plant proteins having an effect of improving serum neutral lipid metabolism or serum cholesterol metabolism, and The present invention relates to a food having an enhanced action of improving serum neutral lipid metabolism or serum cholesterol metabolism. BACKGROUND OF THE INVENTION Serum neutral lipid metabolism or serum cholesterol metabolism is known to be improved by ingestion of vegetable proteins, and for this purpose soy protein Utilization of foods containing plant proteins such as is known. However, foods containing such a vegetable protein do not have a moist feeling and have a poor texture. The texture of the food can be considerably improved by cooking by adding edible oil, shortening, or the like to the vegetable protein, or by cooking such as frying. However, when eating a food whose texture has been improved by this method, fats and oils taken in conjunction with the ingestion of vegetable protein may increase serum neutral lipids or serum cholesterol, eventually causing obesity and associated diseases. Become. Accordingly, an object of the present invention is to improve the texture without adding fats and oils and to enhance the action of improving serum neutral lipid metabolism or serum cholesterol metabolism.
An object of the present invention is to provide a food containing a vegetable protein. Means for Solving the Problems As a result of repeated studies to achieve the above object, the present inventors have found that the addition of a specific phospholipid to vegetable protein improves the texture,
It was also found that this specific phospholipid itself has an effect of improving serum neutral lipid metabolism and serum cholesterol metabolism. [0005] The present invention has been made based on the above-mentioned findings, and comprises serum neutrality comprising 99 to 50% by weight of vegetable protein and 1 to 50% by weight of phosphatidic acid and / or phosphatidate. The present invention provides a food for improving lipid metabolism or serum cholesterol metabolism. Hereinafter, the food for improving serum neutral lipid metabolism or serum cholesterol metabolism of the present invention will be described in detail. As the phosphatidic acid used in the present invention, the constituent fatty acid is a saturated fatty acid having 14 to 24 carbon atoms and a fatty acid having 16 to 22 carbon atoms.
Of unsaturated fatty acids are preferred. The salts constituting the phosphatidate used in the present invention include sodium, potassium, calcium, magnesium, aluminum and ammonium. The content of the above phosphatidic acid or phosphatidate is 1 to 50% by weight, preferably 3 to 50% by weight.
10% by weight. When the content of phosphatidic acid or phosphatidic acid salt is less than 1% by weight, the effect of addition is small, and when it is more than 50% by weight, the amount of vegetable protein in the food becomes small and contains vegetable protein. Not preferred as food. The vegetable protein used in the present invention is not particularly limited, and includes, for example, proteins contained in grains such as wheat, corn, millet, buckwheat, and beans such as soybean. What is necessary is just to select suitably according to the target foodstuff. The food of the present invention may be eaten as it is as a health food, or may be mixed with other food materials and used as a hot cake mix, bread dough, soup powder, noodles, pudding powder and the like. The following are test examples showing the effect of phosphatidic acid or phosphatidate used in the present invention on serum neutral lipid metabolism or serum cholesterol metabolism, and the serum neutral lipid metabolism or serum cholesterol of the present invention. The present invention will be described in more detail with reference to examples of metabolic-improved foods, but the present invention is not limited to these examples. Test Example 1 A 4-week-old male SD rat was fed a diet having the composition shown in Table 1 below and bred for about 3 weeks. That is, the phosphatidic acid-administered group is bred on a diet (PA) containing 5.5% (wt%, hereinafter the same) of phosphatidic acid and 5% soybean triglyceride, and the control group contains 10% soybean triglyceride. The animals were bred on diet (DA) or diet (NA) containing 10% rapeseed triglyceride. No differences were observed in food intake and changes in rat body weight among the three groups during the breeding period. After sacrifice on the 24th day of breeding, serum was separated and serum triglyceride, serum free fatty acid, serum total cholesterol and serum HDL cholesterol were measured. The results are shown in FIGS. 1, 2, and 3.
And FIG. As is clear from FIG. 1, serum triglyceride showed a decreasing tendency in the phosphatidic acid administration group (PA). Moreover, as is clear from FIG. 2, the serum free fatty acids were significantly reduced in the phosphatidic acid-administered group (PA). The above results show that administration of phosphatidic acid to normal rats lowers serum neutral lipids. In addition, as is clear from FIG. 3, there was no significant difference in the serum total cholesterol among the three groups, but as is clear from FIG. 4, the serum HDL cholesterol was increased by the administration of phosphatidic acid.
Serum HDL cholesterol is known to improve arteriosclerosis, and it has been shown that administration of phosphatidic acid improves arteriosclerosis. [Table 1] Test Example 2 In this test, serum neutral lipids and serum cholesterol are known to be genetically elevated in SHC rats (Spon
The present invention is to further elucidate the effect of phosphatidic acid on improving serum neutral lipids and serum cholesterol using taneously hyper cholesterolemic rats). 6-week-old SHC rats were fed a diet having the composition shown in Table 2 below.
The animals were reared for about 3 weeks. That is, the phosphatidic acid administration group is bred on a diet (PA) containing 5.5% phosphatidic acid and 5% soybean triglyceride, and the control group is fed a diet (D) containing 10% soybean triglyceride.
The animals were bred in A). Although there was no difference in food intake between the two groups during the breeding period, the change in the body weight of the rats during the breeding period was greater than that of the control group (DA) as shown in FIG. PA) tended to be low. After sacrifice on the 24th day of breeding, serum was separated and serum triglyceride, serum free fatty acid, serum total cholesterol and serum HDL cholesterol were measured. The results are shown in FIGS. 6, 7, 8 and 9. As is clear from FIG. 6, serum triglyceride showed a remarkably low value in the phosphatidic acid administration group (PA). In addition, as is clear from FIG. 7, serum free fatty acids also showed low values in the phosphatidic acid administration group (PA). It is known that SHC rats have increased synthesis of endogenous neutral lipids and cholesterol. The results of this study suggested that administration of phosphatidic acid reduced the synthesis of endogenous neutral lipids. As is clear from FIG. 8, the serum total cholesterol was significantly reduced in the phosphatidic acid-administered group (PA). Then, using this SHC rat serum, LCAT (Lecithin-Cholesterol Acyltronsferase) was used.
Activity was measured. This enzyme uses phospholipids and free cholesterol in serum and tissues as substrates to form cholesterol esters. The produced cholesterol ester is subjected to the action of HDL in the living body and is excreted from the body through the process of catabolic metabolism in the liver. The LCAT activity was measured by the method of Nagasaki and Akanuma et al. (Nagasaki Toshihide, Akanuma Yasuo, Biochemistry, 46,551,197)
4.) The results are shown in FIG. FIG.
As can be seen from FIG. 0, administration of phosphatidic acid increased serum LCAT activity. That is, administration of phosphatidic acid activates LCAT in SHC rats in which endogenous cholesterol synthesis is increased, and excretes the cholesterol ester generated by metabolism based on the action of HDL and then excretes the body. Was suggested. The serum cholesterol lowering effect of phosphatidic acid was first discovered by the present inventors. [Table 2] Example 1 1.5% sodium phosphatidate was added to isolated soybean protein, and the texture of 1 g of the powder was evaluated. The evaluation was performed by 10 men and women (age 23 to 40). The results are shown in Table 3 below. [Table 3] Example 2 A hot cake mix having the composition shown in Table 4 below was prepared, and the texture after cooking was evaluated using an edible oil (soy triglyceride). Evaluation is 10 men and women each (age 23-
40). The results are shown in Table 5 below. [Table 4] [Table 5] The food of the present invention which improves serum neutral lipid metabolism or serum cholesterol metabolism has the effect of improving the texture without adding fats and oils and improving serum neutral lipid metabolism or serum cholesterol metabolism. Is a food containing a vegetable protein, which is fortified.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows SD on Day 24 of breeding in Test Example 1.
It is a graph which shows the measurement result of the serum triglyceride of a male rat. FIG. 2 shows SD on Day 24 of breeding in Test Example 1.
It is a graph which shows the measurement result of the serum free fatty acid of a male rat. FIG. 3 shows SD on Day 24 of breeding in Test Example 1.
It is a graph which shows the measurement result of the serum total cholesterol of a male rat. FIG. 4 shows SD on Day 24 of breeding in Test Example 1.
It is a graph which shows the measurement result of serum HDL cholesterol of a male rat. FIG. 5 shows SHC during breeding period in Test Example 2.
It is a graph which shows the weight change of a rat. FIG. 6 shows SH on day 24 of breeding in Test Example 2.
It is a graph which shows the measurement result of the serum triglyceride of C rat. FIG. 7 shows SH on day 24 of breeding in Test Example 2.
It is a graph which shows the measurement result of the serum free fatty acid of C rat. FIG. 8 shows SH on day 24 of breeding in Test Example 2.
It is a graph which shows the measurement result of the serum total cholesterol of C rat. FIG. 9 shows SH on Day 24 of breeding in Test Example 2.
It is a graph which shows the measurement result of serum HDL cholesterol of C rat. FIG. 10 is a graph showing the results of measuring the LCAT activity of SHC rats on day 24 of breeding in Test Example 2.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-2-200165 (JP, A) JP-A-1-128919 (JP, A) JP-A-3-297364 (JP, A) JP-A-4- 152861 (JP, A) JP-A-2-42954 (JP, A) JP-A-8-503964 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A23L 1 / 29-1 / 308 A61K 31/66 A61P 3/06

Claims (1)

(57) [Claim 1] 99 to 50% by weight of vegetable protein and 1 to 1 of phosphatidic acid and / or phosphatidic acid salt
A food having improved serum neutral lipid metabolism or serum cholesterol metabolism, characterized by comprising 50% by weight.