JP3331336B2 - Manufacturing method of food and drink to be aseptically filled and packaged - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a sterile (aseptic) has become a serious problem in the food and drink to be filled packaging, Asuriniumu (Arthrinium) genus and / or Chaetomium (Chaetomium) effectively contamination fungal genera The present invention relates to a method for producing aseptically filled food and drink which can be prevented.

[0002]

2. Description of the Related Art In order to distribute processed foods, beverages, and the like filled and packaged in containers at room temperature, hitherto, contents that have been heat-sterilized are hot-filled and sealed in containers while keeping the contents at a high temperature. After filling the containers, retort sterilization in which the entire container is heated has been performed.

On the other hand, in recent years, instead of hot filling and retort sterilization after filling, so-called aseptic (aseptic) filling packaging, in which contents that have been sterilized or disinfected are filled in a sterilized container in an aseptic environment, has been introduced. Is being done.

[0004] The main advantages of aseptic filling and packaging are as follows: 1) The temperature history of the contents is lower than that of hot filling or retort sterilization after filling, and the components are less denatured. There is no need to consider it, and the reason is that the weight and cost of the container can be reduced. In addition, plastic containers and paper-based containers that are rapidly spreading in recent years are non-heat-resistant containers, so retort processing after filling and packaging may not be possible. Spreading rapidly.

[0005] Aseptic filling and packaging require that the contents be sterile, that the packaging material be sterile, and that the atmosphere for filling and sealing the contents into the packaging be sterile. However, although aseptic packaging is technically well established, microbial contamination of products is still frequently reported today.

[0006] Various factors can be considered for the microbial contamination of foods and beverages in the aseptic filling and packaging system. One of the most important factors at present is the presence of microorganisms that are resistant to chemicals used for sterilizing containers. Can be mentioned. In the case of foods and beverages packed aseptically, plastic containers or paper-based containers are frequently used in view of market needs and costs, and these containers are mainly sterilized with hydrogen peroxide, peracetic acid, and hypochlorous acid. Sodium chlorate is used.
However, some microorganisms have resistance to these drugs. As a typical example, Asuriniumu (Arthrinium) genus and Chaetomium (Chaet
omium ) is a fungus of the genus. They actually grow in beverages produced by aseptic filling and packaging, causing troubles which are perceived as foreign in the product.

[0007] As described above, the aseptic filling and packaging system has problems to be solved in the future. In particular, establishment of means for preventing the above-mentioned drug-resistant microorganisms from growing in the product is one of the important problems. .

It is known that licorice or a fraction eluted from licorice with an organic solvent contains an antibacterial substance against bacteria (for example, JP-A-59-46210, JP-A-60-23305). No., JP-A-63-14
No. 5208, Monthly Food Chemical April, Item 94 (1989)). Further, for the mold, Penicillium chrysogenum (Penicillium chrysogenum) (JP 59-46210 JP), Mucor pusillus (M
ucro pusillus ) (JP-A-63-145208)
It is known that the organic solvent extract of licorice has antibacterial properties for the two types.

Glabridin; 3- (2 ′, 4′-di
hydroxyphenyl) -8-dimethylpyrano [8, 7-e] chroman)
Is a kind of isoflavane, and is contained in the root or rhizome of Glycyrrhiza glabra Linne var. (Commonly known as Russia, Afghan, Licorice) which is a kind of licorice in nature. Regarding the physiological activity of grabradine, bacteria ( Staphylococcus aureus , Bacillus subtilis
), Yeast ( Saccharomyces cerevisae , Candida util
is ) and mold ( Mucro pusillus , Aspergillus niger)
) (Chem. Pharm. Bull. 37 (9) 2
528-2530 (1989)), antioxidant action (JP-A-2-2337)
No. 95), a melanin production inhibitory action (Japanese Patent Application Laid-Open No. 1-31).
No. 1011) is known. However, glabridin is Asuriniumu (Arthrinium) genus and Chaetomium (Chaetomium) to have antimicrobial properties against fungi of the genus are not known at all until now.

[0010]

SUMMARY OF THE INVENTION It is an object of the present invention to suppress the growth of microorganisms having resistance to a drug used for sterilizing filling and packaging equipment and containers in food and drink which are aseptically filled and packaged. Another object of the present invention is to effectively prevent troubles such as deterioration of products and generation of foreign substances due to these microorganisms.

[0011]

Means for Solving the Problems The present inventors have solved the above problems.
As a result of intensive studies to solve
But used for sterilization of filling and packaging equipment and containers even at very low concentrations
Earthlinium (Arthrinium
 Genus and ketomium (Chaetomium ) Growth of fungi of the genus
The present invention based on this finding.
completed.

That is, the present invention according to claim 1 provides a peracid
Hydrogen hydride, sodium hypochlorite, peracetic acid, or these
Mixed with a sterilizing agent consisting of material in aseptic filling packaged as food and drink, Asurini <br/> um resistant to the sterilizing agent (Arthrinium) genus and / or Chaetomium (Chaet
omium ) The present invention provides a method for producing aseptically filled food and drink, characterized by adding glabridine as an antifungal agent against fungi of the genus Omium . The present invention according to claim 2, Asuriniumu (Arthrinium) genus fungi, Asuriniumu-Sakkari (Arthrinium sacchari
i ) is a fungus of the genus Chaetomium ,
Chaetomium & Funikora (Chaetomium funicola) a method for producing a food or drink that is aseptic filling and packaging of claim 1, wherein there is provided a. According to a third aspect of the present invention, there is provided a method for producing aseptically filled food or drink as described in claim 1 or 2, wherein the food or drink is a low-acid beverage having a pH of 4.6 or more. The present invention according to claim 4 provides the glabridine aseptically filled food and drink in a range of 1.6 to 10;
It is intended to provide a method for producing a food or drink aseptically filled and packaged according to any one of claims 1 to 3, wherein 0 ppm is added. The method according to claim 5, wherein the low-acid beverage is a tea beverage, a mixed tea beverage, a barley tea beverage, or mineral water. Is provided. The present invention according to claim 6 provides a method for producing a food or drink aseptically filled and packaged according to any one of claims 1 to 5, wherein the container used for aseptic filling and packaging is a non-heat-resistant container. . The present invention according to claim 7 provides a method for producing a food or drink aseptically filled and packed according to any one of claims 1 to 6, wherein the container used for aseptic filling and packaging is a transparent container.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail. The method for producing a food or drink aseptically filled and packaged according to the present invention according to claim 1, comprising: hydrogen peroxide, sodium hypochlorite,
Use a disinfectant consisting of peracetic acid or a mixture of these
The food or drink that is aseptic filling packaging have, resistant to the sterilizing agent
It is characterized by adding glabridine as an antifungal agent against fungi belonging to the genus Arthrinium and / or Chaetomium having the property.

[0014] Glabridin (3- (2 ', 4'-dihydroxyphenyl) -8-dimethy) used in the method of the present invention.
lpyrano [8,7-e] chroman) is a kind of isoflavane and has the following structure.

[0015]

Embedded image

The glabridine used in the method of the present invention may be derived from a natural product or a synthetic product. Examples of glabridine derived from natural products include, for example, Glycyrrhiza glabra Linne var.
It can be purified from roots and rhizomes of Russian, Afghan, and Licorice by a known method (for example, a method described in JP-A-1-3111011).

[0017] This glabridine is used as an earthlinium ( A
rthrinium) genus and / or Chaetomium (Chaetomium)
The present inventors have found for the first time that they have excellent antibacterial properties against fungi of the genus Genus. In particular, as described in claim 2, Arthriniu Sacchari ( Arthriniu
m saccharii ) and ketomium funicola ( Chaetomiu)
m. funicola ) was found to exhibit high antibacterial properties. Asuriniumu (Arthrinium) fungal genera and / or Chaetomium (Chaetomium) genus, since it has a resistance to drugs used in the sterilization of filling and packaging machine, containers, its growth in food that is conventional aseptic filling and packaging It was difficult to properly prevent. However, by employing the method of the present invention, it is possible to effectively prevent the growth of these fungi in foods and the like that are aseptically filled and packaged.

The aseptic filling and packaging generally refers to a system for filling sterilized or sterilized contents into a sterilized container in a sterile environment. In addition, aseptic in aseptic filling and packaging is considered to mean commercial aseptic, and commercial aseptic as used herein refers to microorganisms that are free of food poisoning bacteria and pathogens and that cause rot and economic loss under normal distribution. Is defined as non-existent ("State-of-the-art in sterile packaging and sterilization technology", published by Science Forum). The aseptic filling and packaging in the present invention also complies with this definition.

In the method of the present invention, the process of aseptic filling and packaging can be performed basically in the same manner as the currently used general process of aseptic filling and packaging.

First, the food or drink as the contents is sterilized or sterilized by heating, high pressure treatment, ultraviolet irradiation, radiation irradiation, microfiltration or the like before filling the container. As specific conditions and the like of these processes, conditions and the like in a generally performed method can be appropriately adopted.

The filling and packaging machine is sterilized in advance by a heat treatment with heated steam or hot air or a treatment with various chemicals such as hydrogen peroxide. The atmosphere for aseptic filling and packaging is HEP
By performing the treatment in a clean room using an A filter or the like, it is possible to maintain an aseptic environment. However, the clean room is not completely sterile, as is classified into grades such as class 10 and class 100, depending on the number of dusts present in the atmosphere. It is important to note that bacteria are always floating in the atmosphere. It is assumed.

Means for sterilizing the container generally include heating, ultraviolet irradiation, radiation irradiation, and chemical treatment with ethyl alcohol, hydrogen peroxide, sodium hypochlorite, peracetic acid, ozone, ethylene oxide and the like. Of these, in the case of non-heat-resistant paper-based containers and plastic containers, which are mainly used as containers for aseptic filling and packaging in recent years, hydrogen peroxide is difficult to sterilize by heat or ultraviolet light. Sterilization with sodium hypochlorite, peracetic acid, or a mixture thereof has been performed.

The present invention comprises such a hydrogen peroxide, sodium hypochlorite, peracetic acid, or a mixture thereof .
It is intended for aseptic filling and packaging by a sterilization treatment using a sterilizing agent . That is, the present invention is directed to a case where hydrogen peroxide, sodium hypochlorite, peracetic acid, or a mixture thereof is used for sterilization of a filling and packaging machine and a container, particularly in aseptic filling and packaging . In addition, it is difficult to sterilize by heat, ultraviolet rays, etc., and it is necessary to use hydrogen peroxide, sodium hypochlorite, peracetic acid, or a mixture thereof for sterilization. Non-heat-resistant paper-based containers or plastic containers Is particularly suitable for carrying out the present invention. In such a case, by applying the method of the present invention, it is possible to suppress the growth of fungi having resistance to hydrogen peroxide and the like, and effectively prevent troubles such as deterioration of products and generation of foreign substances due to these fungi. can do.

The food or drink, that is, food and drink, which is the object of the method of the present invention according to claim 1 is not particularly limited as long as it can be aseptically filled and packaged. Examples of the food include cooked rice, rice cake, tofu, cream, yogurt, jelly, bavaroa, mousse, soup, sauce, vinegar, ketchup, mayonnaise, and various extracts.

Examples of the drink include carbonated drinks such as cola and cider, fruit juice drinks, sports drinks, and the like.
Acidified beverages (pH less than 4.6) such as pasteurized lactic acid bacteria drinks, lemon tea, tomato juice, vegetable juice, and dairy products, coffee, cocoa, chocolate, teas, mixed tea,
Barley tea, soy milk, low-acid beverages such as mineral water (p
H4.6 or more).

[0026] Among these foods and drinks, as described in claim 3, in particular, the genus Arthrinium and / or
Or from Chaetomium (Chaetomium) of points susceptible to damage by fungi, low acid beverages (pH 4.6 or higher) is preferred. In addition, among low-acid beverages (pH 4.6 or more),
Non-heat-resistant plastic containers are frequently used as filling containers, and since the containers and beverages themselves are transparent, the above fungi are easily recognized as foreign substances from outside the containers, such as teas, mixed tea, barley tea, and mineral water. Particularly preferred.

Representative examples of the above-mentioned teas include fermented tea (black tea), semi-fermented tea (oolong tea, etc.), and unfermented tea (green tea) using leaves and stems of the Camellia family (Camellia sinensis). Etc.), post-fermented tea (such as Puer tea) and mixtures thereof, and those obtained by mixing milk with them.
In recent years, yellowish tea using walnut plants as a raw material, bean tea using rosaceae plants, and the like have also been distributed as products packed in aseptic filling containers. In the present invention, these are also included in teas.

The mixed tea is a mixture of the above teas and other raw materials. Other raw materials include, for example, brown rice, and barley such as barley, wheat, and pigeon barley;
Medicinal plants such as ginseng; and various herbs.

In the method of the present invention, glabridine is desirably added in advance to food or drink, which is the content before aseptic filling and packaging. The addition amount at this time is appropriately adjusted depending on the kind of the contents, etc., but is preferably 1.6 ppm or more, and more preferably 6.3 ppm or more. On the other hand, the upper limit of the amount of glabridine is not particularly limited, but if it exceeds 50 ppm, the flavor of foods and beverages will feel slightly uncomfortable, and if it exceeds 100 ppm, these flavors will be considerably impaired. It is good to use it in the range which does not exceed.

Since glabridine is insoluble in water, when it is added to foods and drinks aseptically filled and packaged, it may be used in the form of monoglyceride, monoglyceride acetate, lactate monoglyceride, citrate monoglyceride, diacetyltartaric acid monoglyceride, succinic acid monoglyceride, and monoglyceride tartrate. , Stearyl monoglyceride citrate, polyglycerin fatty acid ester, polyglycerin condensed ricinoleate, ammonium monoglyceride phosphate, sodium monoglyceride phosphate, sorbitan tristearate, sorbitan monolaurate, sorbitan monooleate, sucrose fatty acid ester, Sucrose glyceride, propylene glycol fatty acid ester, lecithin, hydroxylated lecithin, stearyl lactic acid Umm, stearyl sodium lactate,
Surfactants or emulsifiers such as cholic acid, deoxycholic acid, stearic acid citrate, stearic acid tartrate, lactate fatty acid ester, sodium dioctylsulfosuccinate, sodium lauryl lactate, sodium stearyl fumarate, alone or in combination of two or more Should be used in combination.

Water, acetone, methyl alcohol,
It is also possible to separately formulate glabridine using various solvents such as ethyl alcohol and propylene glycol, and / or the above-mentioned surfactant or emulsifier, and use it in the production of food or drink.

Gravlidine alone can be used as an earthlinium ( A
Rthrinium) genus and Chaetomium (Chaetomium) genus sufficiently inhibit the growth of fungi, purposes and applications, depending on the type of product, benzoic acid, sodium benzoate, parahydroxybenzoate isobutyl parahydroxybenzoate isopropyl, parahydroxybenzoate Preservatives such as ethyl, butyl paraoxybenzoate, propyl paraoxybenzoate, sorbic acid and potassium sorbate may be appropriately selected and used in combination.

In the present invention, a paper-based container or a plastic container, which is mainly used as a container for aseptic filling and packaging in recent years, is preferably used, but a glass container, a metal can or the like may be used.

Here, the paper-based container is a combination of paper and a metal such as aluminum; polyethylene, polyester, vinylidene chloride, ethylene vinyl alcohol copolymer resin, synthetic polyamide resin, plastic such as polyethylene terephthalate (PET); It is a container made of material.

The plastic container is made of PE
There are trays, bottles, bags for bag-in-boxes, potion bags, caps and films for sealing them, using T, high-density polyethylene, polypropylene, polystyrene and the like.

The paper-based container and the plastic container are
What does not have heat resistance is currently the mainstream from the viewpoint of cost and the like, but in the present invention, as described in claim 6, such non-heat resistant one is preferable. this is,
The method of the present invention is based on the object of suppressing the growth of mold having resistance to a drug used for sterilization when aseptically filling and packaging food and drink in such a non-heat-resistant container. . Here, the non-heat-resistant container means 95
A heating condition of 10 ° C. for 10 minutes or more is equivalent to a container in which the original performance of the container, particularly the sealing property and the shape, are impaired.

When transparent or translucent low-acid beverages such as tea, mixed tea, barley tea and mineral water, which are particularly preferred in the present invention, are aseptically filled and packaged,
As described in claim 7, the use of a transparent container is preferable because the effects of the present invention are further emphasized. That is, when aseptically filling and packaging a transparent or translucent low-acid beverage such as teas, mixed tea, barley tea and mineral water, a transparent container is often used, and the fungus is easily recognized as a foreign substance. In such a case,
The invention is very suitable.

[0038]

The present invention will be described below with reference to production examples and examples, but the present invention is not limited by these examples.

Production Example 1 (Preparation of Gravlidine) 5.0 g of a licorice oil extract was dissolved in a small amount of ethyl acetate.
This was converted to silica gel (Wakogel C-200, Wa
(made by Ko Ltd.) and dried to obtain a gel powder. This gel powder is suspended in hexane, and Wakog
el C-200 column (3.0 cm × 13 cm, solvent: hexane), 170 mL per fraction,
Ethyl acetate concentration in hexane is 10%, 20%, 30%
% And 40%. The fraction eluted with 60% hexane: 40% ethyl acetate was concentrated and dried by an evaporator to obtain 2.1 g of a viscous substance.

This viscous substance was dissolved in a small amount of chloroform, and the mixture was subjected to a Wakogel C-200 column (2.2 cm ×
23 cm, solvent: chloroform), 50 mL of 1
After washing with 00% chloroform and 270 mL of 99% chloroform: 1% methanol, fractionation was performed by elution with 99% chloroform: 1% methanol. The fractions in which only a single brown spot appeared on TLC (thin layer chromatography) were combined, concentrated and dried by an evaporator, and then dissolved in toluene to purify crystals. The crystals were collected and subjected to NMR and mass spectrometry to confirm that they were glabridine.

Preparation Example 2 (Preparation of glabridine preparation) 1 g of glabridine prepared by the method of Preparation Example 1 was mixed with a mixed solvent of ethanol-propylene glycol (1: 1) 60
After dissolving in mL, 3 g of an emulsifier (trade name: L1695, manufactured by Mitsubishi Chemical Foods, sucrose laurate) was added.
Distilled water was added to this solution to make the total amount 100 mL, thereby preparing a glabridine preparation.

Example 1 10% containing glabridine prepared by the method of Production Example 1
20 μL of DMSO and 10 μL / mL of chloramphene
Potato dextrose agar medium containing Nicole (Niss
0.4 mL of a 24-well plate for tissue culture (ICN)
(Made by Biomedical) in each well. On this medium
To Earthlinium Sakari (Arthrinium saccharii
) And ketonium funicola (Chaetomium funicola
) Of 5 μL of spore solution (about 200 spores)
After culturing at 30 ° C. for 72 hours, the growth of these fungi was observed.
I thought. The results are shown in Table 1.

[0043]

[Table 1] Table 1 (Mold growth)

As is evident from Table 1, when the concentration of glabridine was 1.6 ppm or more, the growth of all fungi was remarkably suppressed. In particular, when the concentration was 6.3 ppm or more, they did not grow at all. Thus, it was found that glabridine, even at a very low concentration, inhibits the growth of fungi of the genus Earthrinium and the genus Ketomium.

Example 2 (Production of Green Tea Beverage) 30 g of green tea was extracted with 900 g of ion-exchanged water at 70 ° C. for 5 minutes, and then filtered through filter paper (No. 2, Advantech) to remove tea leaves. , 800 g of green tea extract (pH 6.0, Brix 1.1 °, tannin concentration 2
70 mg / 100 mL). 30 g of the green tea extract
℃ below the drinking concentration (tannin concentration 60mg /
100 mL), and dilute with deionized water.
Ascorbic acid (200 ppm) and the glabridine preparation prepared by the method of Production Example 2 were prepared at a glabridine concentration of 2 pp.
m, and sodium bicarbonate was added thereto to obtain a green tea mixture having a pH of 6.0.

The obtained green tea mixture was subjected to a retort sterilization treatment (121 ° C., 7 minutes) and then cooled to 70 ° C. This was mixed with P-3 Oxonia Active (Ecolab, manufactured in advance to a peracetic acid concentration of 1500 ppm).
After sterilization at 40 ° C. for 30 seconds using 5% peracetic acid), a 500 mL PET bottle washed with sterilized water was filled in a clean bench (class 100). The bottle was sealed with a plastic cap that had been subjected to the same sterilization treatment as the bottle, to obtain a green tea beverage in aseptic filling and packaging.

Example 3 (Production of Black Tea Beverage) Black tea was removed by extracting 30 g of black tea with 900 g of ion-exchanged water at 70 ° C. for 5 minutes, followed by filtration through a filter paper (No. 2, Advantech). , 800 g of black tea extract (pH 5.5, Brix 1.1 °, tannin concentration 3
00 mg / 100 mL). 30% of the black tea extract
℃, the extract, milk, sugar, emulsifier (DK
Ester E-6000, manufactured by Daiichi Kogyo Seiyaku) and ion-exchanged water in a weight ratio of 33: 25: 5: 0.2:
In addition, the glabridine preparation prepared by the method of Production Example 2 was added to a glabridine concentration of 10 p.
pm, and sodium bicarbonate was added to adjust the pH to 6.8 to obtain a milk tea mixture.

The resulting milk tea mixture was stirred while being heated to 60 ° C., and then homogenized (homogeneous pressure: 200 kg / cm ² ) with a homogenizer. This was subjected to a retort sterilization treatment (121 ° C., 20 minutes) and then cooled to room temperature. Thereafter, in the same manner as in Example 2, a 500 mL P
The ET bottle was aseptically filled and packed.

Example 4 (Production of Mixed Tea) A mixed tea was trial-produced using a raw material containing 65% of oolong tea, 20% of black tea, 5% of jasmine, 4% of skin, 4% of hibiscus and 2% of banaba. 30 g of the obtained mixed tea is 9
Extraction was performed with 900 g of ion-exchanged water at 0 ° C. for 10 minutes, followed by filtration with a filter paper (No. 2, manufactured by Advantech) to remove tea leaves, and 820 g of a mixed tea extract (pH
4.5, Brix 0.9 °, tannin concentration 150 mg /
100 mL). The mixed tea extract was cooled to 30 ° C. or lower, diluted with ion-exchanged water to a drinking concentration (Brix 0.2 °), and L-ascorbic acid (200 p.
pm) and the glabridine preparation prepared by the method of Production Example 2 so that the glabridine concentration becomes 5 ppm,
Sodium bicarbonate was added thereto to obtain a mixed tea mixture having a pH of 6.0.

This was subjected to a retort sterilization treatment (121 ° C., 7 minutes) and then cooled to 30 ° C. Then, it was aseptically filled and packaged in a 500 mL PET bottle in the same manner as in Example 2.

Example 5 (Production of Barley Tea Beverage) Barley tea barley (80 g) was extracted with 1600 g of ion-exchanged water at 90 ° C. for 30 minutes, and then filtered through filter paper (No. 2, Advantech) to remove the raw material residue. Remove and 1
440 g of barley tea extract (pH 4.9, Brix 0.6
°). The barley tea extract is cooled to 30 ° C. or less,
It was diluted with ion-exchanged water to a drinking concentration (Brix 0.4 °). L-Ascorbic acid (200 ppm) and the glabridine preparation prepared by the method of Production Example 2 were added so that the glabridine concentration became 10 ppm, and sodium hydrogen carbonate was added thereto to obtain a green tea mixture having a pH of 6.0.

This was subjected to a retort sterilization treatment (123 ° C., 20
Minutes) and then cooled to room temperature. Then, it was aseptically filled and packaged in a 500 mL PET bottle in the same manner as in Example 2.

Example 6 (Production of coffee beverage) Coffee extract (trade name: coffee extract M-0-2)
0, Brix20, manufactured by Takasago Coffee), milk, sugar, emulsifier (trade name: Sunsoft Super V-103, manufactured by Taiyo Kagaku) and ion-exchanged water in a weight ratio of 4.5:
0: 5: 0.2: 80.3, and the glabridine preparation prepared by the method of Production Example 2 was added so that the glabridine concentration was 25 ppm.
An appropriate amount of sodium bicarbonate was added thereto, and the pH was adjusted to 6.
8 was obtained.

This coffee mixture was stirred while being heated to 60 ° C., and then homogenized (homogeneous pressure: 200 kg / cm ² ) with a homogenizer. This was subjected to a retort sterilization treatment (121 ° C., 20 minutes), and then cooled to room temperature with water.
Thereafter, in the same manner as in Example 2, a 500 mL PET
Bottles were aseptically filled and packaged.

[0055]

According to the present invention, in foods and drinks to be aseptically filled and packaged, microorganisms having resistance to a drug used for sterilization of a filling and packaging apparatus and a container are prevented from growing in a product. Troubles such as deterioration of the product and generation of foreign matter can be prevented. That is, according to the present invention, Asuriniumu has become a serious problem in the food and drink that is aseptic filling and packaging (Arthrinium) genus and / or Ketoniumu (Chaetomium) contamination by fungal genera, especially Asuriniumu-Sakkari (Arthrinium sacc
harii ) and / or Ketomium funicola
ium funicola ) can be effectively prevented. In addition, glabridine exhibits a remarkable effect even in a very low concentration or a very low concentration. Thus, according to the present invention, the safety and reliability of these products can be dramatically improved without impairing the flavor of foods and beverages to be aseptically filled and packaged.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI B65D 81/28 A23L 2/00 P Examiner Takeshi Ukai (56) References JP-A-2-223795 (JP, A) Chem. Pharm. Bull. , 37 (1989), p. 2528-2530 (58) Field surveyed (Int. Cl. ⁷ , DB name) A23L 3/3544 A23L 2/44 JICST file (JOIS)

Claims (7)

(57) [Claims] (1) hydrogen peroxide, sodium hypochlorite, peroxide
Using acetic acid or a disinfectant consisting of a mixture of these
The aseptic filling packaged as foods or drinks Te, resistant to the sterilizing agent
As antifungal agents against Asuriniumu (Arthrinium) fungal genera and / or Chaetomium (Chaetomium) genus with, and characterized by the addition of glabridin, manufacturing method of food or drink that is aseptic filling and packaging. 2. Earthlinium (Arthrinium Genus true
Bacteria are earthly sacchari (Arthrinium saccha
rii ) And ketomium (Chaetomium ) Genus fungi
But, Ketomium Funicola (Chaetomium funicola
 2. The method of claim 1, wherein the food or beverage is aseptically filled and packaged.
Production method. 3. The method according to claim 1, wherein the food or beverage is a low-acid beverage having a pH of 4.6 or more. 4. The food or drink to be aseptically filled and packaged in an amount of 1.6 to 100 ppm of glabridin.
The method for producing a food or drink aseptically filled and packaged according to any one of the above. 5. The low-acid beverage is a tea beverage, a mixed tea beverage,
The method for producing aseptically filled food or drink according to any one of claims 1 to 4, which is barley tea beverage or mineral water. 6. The method according to claim 1, wherein the container used for aseptic filling and packaging is a non-heat-resistant container. 7. The method according to claim 1, wherein the container used for aseptic filling and packaging is a transparent container.