JP3320879B2 - Anti-gastrin agent - Google Patents
Anti-gastrin agentInfo
- Publication number
- JP3320879B2 JP3320879B2 JP34841193A JP34841193A JP3320879B2 JP 3320879 B2 JP3320879 B2 JP 3320879B2 JP 34841193 A JP34841193 A JP 34841193A JP 34841193 A JP34841193 A JP 34841193A JP 3320879 B2 JP3320879 B2 JP 3320879B2
- Authority
- JP
- Japan
- Prior art keywords
- extract
- fraction
- gastrin
- water
- methanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- Medicines Containing Plant Substances (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、抗ガストリン剤に関す
る。The present invention relates to an anti-gastrin agent.
【０００２】[0002]
【発明の背景技術】紅景天は、チベット等の高山地帯に
生育する多年生草本植物、べんけいそう科のＲｈｏｄｉ
ｏｌａ ｒｏｓｅａ Ｌ属植物の根および根茎で、中国
では民間薬として、抗疲労、抗無酸素症、抗毒、貧血改
善の目的で使用されている。また中国では、スポーツ選
手の酸欠、疲労回復の目的で、紅景天エキスを含有した
健康飲料が市販されている。しかしながら、紅景天およ
びその抽出エキスが抗ガストリン作用による消化性潰瘍
の改善に有効であることは知られていない。BACKGROUND OF THE INVENTION Hongjingtian is a perennial herbaceous plant that grows in alpine areas such as Tibet, and Rhodi, a member of the family Benikeiaceae.
Ola rosea Roots and rhizomes of the L genus plant, which are used as folk medicine in China for the purpose of anti-fatigue, anti-anoxia, anti-poisoning and amelioration of anemia. In China, health drinks containing Hongjing Tian extract are marketed for the purpose of relieving athletes of lack of oxygen and fatigue. However, it is not known that Hongjingtian and its extract are effective for improving peptic ulcer by anti-gastrin action.
【０００３】一方、現在市場に存在している消化性潰瘍
の治療剤は、副作用、効力、治療後の再発等の面で問題
があり、すべてに満足のいくものではない。On the other hand, the therapeutic agents for peptic ulcers currently on the market have problems in side effects, efficacy, recurrence after treatment, etc., and are not all satisfactory.
【０００４】[0004]
【発明が解決しようとする課題】そこで、本発明の目的
は、抗ガストリン作用による消化性潰瘍の改善に有効で
ある薬剤を提供することにある。SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a drug which is effective for improving peptic ulcer by antigastrin action.
【０００５】[0005]
【課題を解決するための手段】本発明者は、上記課題を
解決するために鋭意検討を行った結果、紅景天およびそ
の抽出エキスに、抗ガストリン作用による消化性潰瘍の
改善に有効であることを見い出し、本発明を完成するに
至った。Means for Solving the Problems The present inventor has made intensive studies to solve the above-mentioned problems, and as a result, it is effective to improve the peptic ulcer by anti-gastrin action on Hongjingtian and its extract. This led to the completion of the present invention.
【０００６】すなわち、本発明は、紅景天またはその抽
出エキスを有効成分として含有することを特徴とする抗
ガストリン剤に関する。[0006] That is, the present invention relates to an anti-gastrin agent characterized by containing Benkeiten or an extract thereof as an active ingredient.
【０００７】以下、本発明について詳しく説明する。本
発明の紅景天とは、べんけいそう科のＲｈｏｄｉｏｌａ
ｒｏｓｅａ Ｌ属植物の根および根茎を乾燥させたも
のをいう。また、紅景天抽出エキスとは、紅景天を、メ
タノールやエタノールなどのアルコール類や水等の抽出
溶媒によって抽出されたエキスである。Hereinafter, the present invention will be described in detail. The Red Kageten of the present invention is Rhodiola of the family
It refers to a dried root and rhizome of a rosea L genus plant. In addition, the red-kageten extract is an extract obtained by extracting red-kageten with an extraction solvent such as alcohols such as methanol and ethanol and water.
【０００８】抽出方法、抽出条件は、任意に選択、設定
することができる。次に、得られた抽出エキスを植物成
分の精製、単離に用いられる慣用手段、たとえば各種溶
媒間の分配クロマトグラフィー等によって分画する。分
配クロマトグラフィー処理に用いられる各種溶媒として
は、水、またはアルコール類（メタノール、エタノー
ル、ブタノール等）、エステル類（酢酸エチル、酢酸ブ
チル等）、エーテル類（エチルエーテル、ジオキサン
等）、炭化水素（ベンゼン、トルエン、ヘキサン等）、
ハロゲン化炭化水素（クロロホルム、塩化エチレン等）
またはこれらの混合溶媒を用いればよい。The extraction method and extraction conditions can be arbitrarily selected and set. Next, the obtained extract is fractionated by conventional means used for purification and isolation of plant components, for example, partition chromatography between various solvents. Various solvents used for partition chromatography include water or alcohols (eg, methanol, ethanol, butanol), esters (eg, ethyl acetate, butyl acetate), ethers (eg, ethyl ether, dioxane), hydrocarbons (eg, Benzene, toluene, hexane, etc.),
Halogenated hydrocarbons (chloroform, ethylene chloride, etc.)
Alternatively, a mixed solvent thereof may be used.
【０００９】[0009]
【実施例】以下に、紅景天抽出エキスの抽出例、抽出エ
キス分画例、薬理試験例を示すが、本発明はこれに限定
されるものではない。[Examples] Examples of extraction, extract fractionation, and pharmacological test examples of Hongjingtian extract are shown below, but the present invention is not limited thereto.
【００１０】（紅景天エキスの抽出例） 紅景天２２０ｇを室温にて、メタノール１５００ｍｌで
２回抽出を行った後、メタノールを留去し乾燥させ、メ
タノール抽出物９ｇを得た。（収率４．１％）(Extraction Example of Red Kageten Extract) After 220 g of Red Kageten was extracted twice with 1500 ml of methanol at room temperature, methanol was distilled off and dried to obtain 9 g of a methanol extract. (Yield 4.1%)
【００１１】（抽出エキス分画例） メタノール抽出物の乾燥品５ｇを２５０ｍｌの蒸留水に
溶かし、先ず２５０ｍｌのジエチルエーテルで４回抽出
し、これをエーテル画分とする。次に残った水層に対し
て、２５０ｍｌの酢酸エチルで４回抽出し、これを酢酸
エチル画分とする。更に同様に残った水層に対して、２
５０ｍｌの水飽和ｎ−ブタノールで４回抽出し、これを
ブタノール画分とし、残った画分を水画分とする。各画
分の溶媒を留去後、凍結乾燥させて固形物を得た。それ
ぞれの画分の固形物の収率は、紅景天のメタノール抽出
物乾燥品５ｇに対して、エーテル画分２１重量％、酢酸
エチル画分１１重量％、ブタノール画分３１重量％、水
画分３７重量％であった。更に水画分については、限外
濾過（分画分子量１０００）を行い、高分子量側と低分
子量側に分画し、凍結乾燥させて固形物を得た。その結
果、水画分の固形物に対して、高分子量側の固形物の収
率５４％、低分子量側の固形物４６重量％が得られた。(Example of Extractive Extract Fractionation) 5 g of the dried methanol extract is dissolved in 250 ml of distilled water, and firstly extracted four times with 250 ml of diethyl ether to obtain an ether fraction. Next, the remaining aqueous layer was extracted four times with 250 ml of ethyl acetate, and this was used as an ethyl acetate fraction. Further, for the remaining water layer,
Extract four times with 50 ml of water-saturated n-butanol, use this as the butanol fraction, and let the remaining fraction be the water fraction. After evaporating the solvent of each fraction, it was freeze-dried to obtain a solid. The yield of solids in each fraction was as follows: 5 g of a dried methanol extract of Hongjingtian, 21% by weight of an ether fraction, 11% by weight of an ethyl acetate fraction, 31% by weight of a butanol fraction, and 31% by weight of a water fraction. 37% by weight. Further, the water fraction was subjected to ultrafiltration (fraction molecular weight: 1,000), fractionated into a high molecular weight side and a low molecular weight side, and freeze-dried to obtain a solid. As a result, the yield of the solid matter on the high molecular weight side was 54% and the solid matter on the low molecular weight side was 46% by weight based on the solid matter of the water fraction.
【００１２】 〔胃酸分泌の抑制試験（抗ガストリン試験）〕 ２日間絶食させたラットに対して、紅景天エキスのメタ
ノール抽出品固形物および各抽出画分固形物の下記の用
量を蒸留水に溶解し、腹腔内投与した。その３０分後に
ペンタガストリン（５μｇ／ｋｇ）を皮下投与する。更
に、生理食塩水２ｍｌを経口投与し、５分後に胃を抽出
し胃液を採取する。採取した胃液を遠心後、その上清に
ついて０．０１ＮのＮａＯＨ溶液を用い、中和滴定を行
い、ブランクと比較することで胃液分泌の抑制率を求め
る。なお、対照品として、消化性潰瘍治療剤シメチジン
５ｍｇ／ｋｇを腹腔内投与し、以下同様な操作を行っ
た。その結果を表１に示す。[Stomach Acid Secretion Inhibition Test (Anti-Gastrin Test)] For rats fasted for 2 days, the following doses of the solids of the methanol extract of Kungjing heaven extract and the solids of each extracted fraction were added to distilled water. Dissolved and administered intraperitoneally. Thirty minutes later, pentagastrin (5 μg / kg) is administered subcutaneously. Further, 2 ml of physiological saline is orally administered, and after 5 minutes, the stomach is extracted and gastric juice is collected. After the collected gastric juice is centrifuged, the supernatant is subjected to neutralization titration using a 0.01 N NaOH solution, and the rate of inhibition of gastric juice secretion is determined by comparing with a blank. As a control, 5 mg / kg of the peptic ulcer therapeutic agent cimetidine was intraperitoneally administered, and the same operation was performed thereafter. Table 1 shows the results.
【００１３】[0013]
【表１】 [Table 1]
【００１４】以上の結果から、特に紅景天の水画分固形
物および水画分の高分子量側画分固形物にシメチジンほ
ど作用は強くないが、抗ガストリン作用があることが認
められた。[0014] From the above results, it was confirmed that the water fraction solids of Hongjingtian and the high molecular weight side fraction solids of the water fraction are not as strong as cimetidine, but have an anti-gastrin effect.
【００１５】[0015]
【効果】本発明によって、紅景天およびその抽出エキス
が、抗ガストリン作用を有することが明らかとなり、紅
景天およびその抽出エキスは抗ガストリン作用による消
化性潰瘍の改善に有効である。According to the present invention, it is clear that Hongjingtian and its extract have an anti-gastrin effect, and it is effective for improving peptic ulcer by antigastrin action.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 ＫＨＩＭ．−ＦＡＲＭ．ＺＨ，1986, Ｖｏｌ．20，Ｎｏ．９，ｐｐ．1107− 1134，（Ｃｍｅｍｉｃａｌ Ａｂｓｔｒ ａｃｔｓ：ａｂｓ．ｎｏ．，106： 60790) (58)調査した分野(Int.Cl.7，ＤＢ名) A61K 35/78 ＢＩＯＳＩＳ（ＤＩＡＬＯＧ) ＣＡ（ＳＴＮ) ＭＥＤＬＩＮＥ（ＳＴＮ)──────────────────────────────────────────────────続 き Continued on the front page (56) Reference KHIM. -FARM. ZH, 1986, Vol. 20, No. 9, pp. 1107- 1134, (Cmemical Abstr acts: abs.no., 106: 60790) (58) investigated the field (Int.Cl. 7, DB name) A61K 35/78 BIOSIS (DIALOG) CA (STN) MEDLINE (STN)
Claims (1)
として含有することを特徴とする抗ガストリン剤。1. An anti-gastrin agent comprising as an active ingredient, Red Keiten or an extract thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34841193A JP3320879B2 (en) | 1993-12-27 | 1993-12-27 | Anti-gastrin agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34841193A JP3320879B2 (en) | 1993-12-27 | 1993-12-27 | Anti-gastrin agent |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002102406A Division JP3377997B2 (en) | 2002-04-04 | 2002-04-04 | Substance P antagonist |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07188039A JPH07188039A (en) | 1995-07-25 |
| JP3320879B2 true JP3320879B2 (en) | 2002-09-03 |
Family
ID=18396837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP34841193A Expired - Fee Related JP3320879B2 (en) | 1993-12-27 | 1993-12-27 | Anti-gastrin agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3320879B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2768621B1 (en) * | 1997-09-22 | 2000-04-07 | Oreal | USE OF AN EXTRACT OF AT LEAST ONE PLANT FROM THE ROSACEA FAMILY |
| JP4633219B2 (en) * | 2000-03-02 | 2011-02-16 | グンゼ株式会社 | α-Amylase inhibitor |
| ITMI20031534A1 (en) * | 2003-07-25 | 2005-01-26 | Marfarma S R L Ora Marfarma Holdin G S P A | COMPOSITIONS FOR THE TREATMENT OF ANXIETY AND RELATED DISORDERS |
-
1993
- 1993-12-27 JP JP34841193A patent/JP3320879B2/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| ＫＨＩＭ．−ＦＡＲＭ．ＺＨ，１９８６，Ｖｏｌ．２０，Ｎｏ．９，ｐｐ．１１０７−１１３４，（Ｃｍｅｍｉｃａｌ Ａｂｓｔｒａｃｔｓ：ａｂｓ．ｎｏ．，１０６：６０７９０） |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07188039A (en) | 1995-07-25 |
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