JP3228534B2 - Composition for lowering blood alcohol concentration - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for lowering blood alcohol concentration, comprising an ethanol-insoluble extract of a tissue culture panax ginseng as an active ingredient. The composition is useful as a medicine, food (especially functional food) and the like.

[0002]

2. Description of the Related Art Alcohol taken orally (drinked) is absorbed from the digestive tract, enters the blood, and is metabolized mainly by the liver. Metabolism occurs in the liver alcohol dehydrogenase system and the microsomal ethanol oxidation system, both of which are oxidized to produce acetaldehyde. The produced acetaldehyde has various adverse effects on the living body, such as hot flushes, increased heart rate, headache, drowsiness, nausea, allergic symptoms,
Causes sickness, hangover, and hepatitis. This acetaldehyde is metabolized to acetic acid by acetaldehyde dehydrogenase in the liver.If the activity of this enzyme is low or the blood alcohol concentration is high, acetaldehyde accumulates and causes poisoning due to the above harmful effects. There is. Therefore, in order to reduce the occurrence of the symptoms as described above even when ingesting alcoholic beverages, a substance that lowers the alcohol concentration in the blood or a substance that promotes metabolism of acetaldehyde or a substance that reduces the toxicity of acetaldehyde can be obtained. It is considered good.

[0003] Such substances include D-penicillamine (Reference 1) and L-
SH compounds such as cysteine and DL-homocysteine,
Vitamin B ₁ and its derivatives and ascorbic acid (Reference 2), L-methionine (Document 3), egg white hydrolyzate as a metabolic enhancer (various amino mixture) (Reference 4), L-a- as toxicity-reducing agent Alanine (Reference 5) and glycyl-glycine (Reference 6) are known as alcohol absorption inhibitors.

On the other hand, Panax ginseng C.
A. Meyer)
It is known for its tonic, tonic, and gastrointestinal functions. Also,
Ginseng has been reported to suppress hepatitis due to long-term administration of alcohol (Reference 7). Further, a blood alcohol concentration lowering effect is known (Reference 8).

[0005] However, Literature 8 merely describes the blood alcohol concentration lowering effect of ginseng extract, and the effect is not very strong. Ginseng is a natural product, and its effects vary.

[0006]

SUMMARY OF THE INVENTION An object of the present invention is to provide a stable and powerful composition for lowering blood alcohol concentration.

[0007]

Means for Solving the Problems In order to achieve the above object, the present inventors have conducted intensive studies, and as a result, it has been found that an ethanol-insoluble substance of a tissue culture panax ginseng extract has a reproducible and extremely excellent blood alcohol. The present inventors have found that they have a concentration lowering effect, and have conducted further studies to complete the present invention. That is, the present invention is a composition for lowering blood alcohol concentration, which comprises an insoluble ethanol extract of a tissue culture panax ginseng extract.

[0008] The origin of the tissue-cultured Panax ginseng used in the present invention is not particularly limited, and may be derived from any of Japan, Korea, China and the like. The method of producing the tissue culture panax ginseng may be in accordance with a method known per se, for example, JP-B-60-4713, JP-B-63.
A method described in JP-A-21470 or a method analogous thereto is exemplified.

In the present invention, the extract of tissue culture Panax ginseng is usually an extract with a polar solvent, preferably a highly polar solvent, but the composition of the present invention is used for foods (particularly functional foods), pharmaceuticals and the like. Considering that, the extract is preferably an aqueous solvent-based extract. As the aqueous solvent, water alone or a mixed solvent of water and ethanol at any ratio is conveniently used. Here, the mixing ratio of water and ethanol is usually 20:80 to 100: 0, preferably 50:50. At the time of extraction, the extraction efficiency is further improved by heating to a range of room temperature to 100 ° C.

The ethanol-insoluble matter of the extract of tissue culture Panax ginseng is a precipitate formed when the above extract is mixed with ethanol. The ethanol-insoluble matter is prepared, for example, as follows. That is, the extract obtained above is preferably concentrated and dried by a usual method, and further, after dissolving the dried product in a small amount of water, adding ethanol, stirring and filtering by a usual method, and collecting a precipitate. Prepared by This method is called an ethanol precipitation method. In this method, it is efficient to pulverize the dried product of the extract mechanically or by ultrasonication, dissolve it in a small amount of water, and then add ethanol. .

The composition of the present invention is useful for food applications, functional foods, food additives and the like. Further, the composition of the present invention is useful as a drug such as a blood alcohol concentration lowering agent for mammals including humans.

The composition of the present invention may be ingested as it is in the form of the above-mentioned precipitate. However, in consideration of ease of ingestion and easiness of processing, it may be once converted into a fine powder by freeze-drying or the like. preferable. In addition, the composition of the present invention may be used together with additives (carriers, excipients, bulking agents, stabilizers, flavoring agents, flavors, other pharmaceutical ingredients, etc.) known per se, for example, beverages, powdered foods, tablets, It may be formed into granules, liquids, powders, capsules and the like, and formulated.

The amount of ethanol-insoluble extract obtained from the extract of tissue culture panax ginseng, which is an active ingredient of the present invention, for obtaining the effect of lowering the alcohol concentration in blood varies greatly depending on the weight and constitution of the person who takes the extract. Although not clearly specified, it is generally desirable that the single dose is in the range of 0.1 to 10 g, preferably 0.3 to 3 g, when converted into the tissue culture panax ginseng as a starting material. The extract is preferably insoluble in ethanol in the range of 0.2 to 5 g.

The timing of ingesting the composition for lowering blood alcohol concentration of the present invention is not particularly limited, and sickness and hangover can be prevented by ingesting the composition before or simultaneously with the ingestion of alcohol. In addition, it is possible to suppress sickness and hangover by taking it after ingesting alcohol.

The composition for lowering blood alcohol concentration of the present invention does not show toxicity to living organisms and is extremely high in safety, as is clear from the following experimental examples.

[0016]

EXAMPLES The present invention will be described below in detail with reference to experimental examples and examples. Reference Example 1 A callus derived from the root of Panax ginseng (from Korea) was used as a seed. The callus was cultured on a solid MS medium containing agar (Murashige-Skoog medium) under normal culture conditions. As a culture solution, an MS medium containing 0.1 ppm of kinetin and 2 ppm of indoleacetic acid was used. 2 L of this culture was sterilized and inoculated with a predetermined amount of the seed tissue. The inoculation amount was 82 g for the first time, 99 g for the second time, and 121 g (the wet weight for the third time). This was shake-cultured at 25 ° C. for 2 weeks with a reciprocating shaker at 90 strokes per minute to obtain tissue-cultured panax ginseng.

Reference Example 2 500 g of the dried tissue culture panax ginseng obtained in Reference Example 1 was immersed in 50% ethanol and extracted at 85 ° C. for 5 hours to obtain an extract. This extract was lyophilized to 207.5 g.
To obtain an extracted dry powder. Further, the extracted dry powder 10
0 g was dissolved in 0.5 L of water. 2.5 L of ethanol was added dropwise under stirring, and the insoluble matter was collected by filtration through Whatman No. 2 filter paper, and dried to obtain 48 g of ethanol precipitate.

Experimental Example 1 Materials: Tissue-cultured Panax ginseng prepared according to Reference Example 1 and 40 g of a dry powder of a cultivated Panax ginseng were used in 80
Extraction with 0 ml of 50% ethanol and lyophilization gave extract powders (41.3%, 41.3% respectively). Further, 10 g of a tissue culture panax ginseng extract powder was dissolved in 50 ml of water, 250 ml of ethanol was added with stirring, and the mixture was filtered after standing, and the precipitate was collected to obtain 4.7 g of an ethanol precipitate.

Experimental method: Wistar male rats (8-10 weeks old) which had been fasted for 24 hours from the day before the test were subjected to 3 g of ethanol precipitate of tissue-cultured panax ginseng, 3 g of the above 50% alcohol extract powder of tissue-cultured panax ginseng, and cultivated panax ginseng 3 g of the above 50% alcohol extract powder was suspended in 15 ml of water, and 5 ml of the powder
Is orally administered. Immediately thereafter, 30% (w / v) of ethanol is orally administered. From ethanol administration, 0, 30,
After 60, 90, 120, and 180 minutes, about 100 μl of blood is collected from the tail vein using a hematocrit tube under anesthesia. The collected blood is centrifuged at 12000 rpm for 5 minutes. To 20 μl of the obtained plasma, 580 μl of 0.33 ml / L perchloric acid is added and mixed. After centrifugation at 3000 rpm for 5 minutes, the amount of ethanol in the supernatant was measured using an ethanol measurement kit (Boehringer Ingelheim Yamanouchi).

Experimental results: As shown in FIG. 1, only the ethanol-insoluble substance of tissue culture panax ginseng showed a very remarkable blood alcohol concentration lowering effect.

Experimental Example 2 Experimental method: A dry powder of ethanol precipitate of tissue culture panax ginseng was administered at a dose of 5 g / kg to three ddY male mice, respectively, and observed for 2 hours to examine acute toxicity. Experimental results: No deaths were observed in the group administered with the dry powder of the ethanol precipitate of tissue culture ginseng. Thus, the composition for lowering blood alcohol concentration of the present invention has extremely low toxicity and high safety.

Example 1 Beverage Tissue cultured ethanol precipitate of Panax ginseng dry powder 1 g Liquid sugar 10 g Organic acid (citric acid) 0.3 g Vitamin C 1 g Cyclodextrin 1 g Flavor 0.5 ml Mix the above ingredients and add water. After making up to 100 ml, it was sterilized by heating to obtain a beverage product.

Example 2 Granules / Powder Tissue-cultured Panax ginseng ethanol precipitate dry powder 1 g Seasoning appropriate amount Cyclodextrin 1 g The above ingredients are blended and mixed well to obtain a powdered food. Granules are prepared by mixing the above components, adding a small amount of water, kneading, sizing and drying. The above amount is taken as one package, and one to three packages are taken at a time.

Example 3 Hard capsules were filled with 0.5 g of a dry powder of ethanol insoluble tissue panax ginseng in ethanol. This capsule is 1
Take 1-6 capsules at a time.

[0025]

Since the composition of the present invention significantly lowers the blood alcohol concentration, it is possible to prevent and prevent sickness and hangover caused by drinking, and to prevent alcohol hepatitis in the long term. It can be used for food and medicine.

[0026]

[Literature]

1. Ngasawa, HT, et al .: Life Sciences vol. 20,
187, 1977 2. Isamu Morii, Keito Tsurumi et al .: Alcohol Studies vol. 11 No.
2: 12, 1974 3. Peter. CJ: Analitical Biochem., Vol. 87, 11
6, 1977 4. JP-A-58-157724 5. JP-A-61-134313 6. JP-A-3-127439 7. Eui, SC, et al .: Korean Biochem. J .: vol.
11 (1), 1, 1978 8. Lee, FC, et al .: Clinical and Experimental
Pharmacology & Physiology: vol. 14, 543, 1987

[Brief description of the drawings]

FIG. 1 is a graph showing that the composition of the present invention exhibits an excellent blood alcohol concentration lowering effect.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-60-188326 (JP, A) JP-A-3-7235 (JP, A) Nihon Pharmaceutical Journal, vol. 93, no. 4, p 261-270, 1989 (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 35/78 BIOSIS (DIALOG) CA (STN)

Claims (3)

(57) [Claims] Claims: 1. A decrease in blood alcohol concentration comprising an ethanol-insoluble substance of a tissue culture panax ginseng extract
Agent . 2. The agent for lowering blood alcohol concentration according to claim 1, wherein the extract of tissue culture panax ginseng is an extract with an aqueous solvent. 3. The agent according to claim 2, wherein the aqueous solvent is water alone or a mixed solvent of water and ethanol.