JP3184708U - Pipette for exhalation / inhalation operation - Google Patents

Abstract

An expiratory / inspiratory operation pipette that can perform a very small sample sample collection and discharge operation, is excellent in economic efficiency, and can effectively prevent cross-contamination.
A pipette that sucks and discharges a specimen sample 8 into and from a tip device 7 by exhalation and inhalation, and is made of a flexible material that is impermeable to gas and liquid and does not itself have elastic resilience. A distal end mounting member for attaching a distal end tool 7 for accommodating the specimen sample 8 is a housing 3 that prevents cross-exchange by separating the inner space into a mouth side space S1 and a tube side space S2 by the diaphragm 4 5 is deviated to the mouthpiece 2 side as much as possible.
[Selection] Figure 2

Description

  The present invention relates to an exhalation / inhalation operation pipette that uses a gas suction / exhaust as a gas suction / exhaust suction source in a tip for receiving a specimen sample.

  Conventionally, in medical and biological research and testing, for example, cells, tissues, culture media, etc. of cells, skin, bones, blood vessels, organs, etc. A driving source that collects and discharges the specimen sample in the tip tool employs various means such as manual operation, electric drive, and breath inhalation / exhaust. In this case, especially when the specimen sample is in the micron unit or the handling amount is extremely small, it is easier to handle, accurate, and economical than the manual or electric one. A tube 02 having a mouthpiece (mouth) 01 that can be gripped by the lips, and a tip attachment member 03 at the tip of the tube 02. A disposable tip 04 is attached to the tip attachment member 03. A so-called exhalation / inspiration pipette (see FIG. 7) is often employed.

  Such a conventional pipette for exhalation / inhalation operation holds the mouthpiece 01 on the lips, inhales, inhales the specimen sample 06 into the sample space 05 of the tip 04, then blows out the breath, The specimen sample 06 is transferred to, for example, a petri dish (not shown) or a microscope or the like. At that time, the air that has contacted the specimen sample 06 in the sample space 05 of the tip tool 04 is sucked into the tube 02 via the tip tool mounting portion 03. Conversely, exhaled air or saliva may enter the tube 02 and come into contact with the specimen sample 06 in the sample space 05 of the tip 04. In this state, there is a possibility that air, exhaled air, or saliva in contact with the previous sample sample 06 remains in the tube 02 even if the tip sample 04 is replaced after the sample sample 06 is discharged from the tip tool 04. There is. In addition, the discharged specimen sample 06 may already be contaminated with breath or saliva.

  Therefore, with this type of conventional structure, even if the tip tool 04 is replaced, it is impossible to prevent complete cross-contamination, and even if washed, 100% sterility and cleanliness are ensured. It is difficult to eradicate cross-contamination, and eventually the equipment itself must be replaced with a new one, which is not economically advantageous.

  As one means for solving such a problem, an airtight housing 07 is attached to the distal end portion of the tube 02, a diaphragm 08 is disposed in the housing 07, and the space 09 in the housing 07 is moved to the distal end side 09A. And the suction side 09B are separated, and the air on the distal end device 04 side moves to the tube 02 and the mouthpiece 01 side, and conversely, the exhalation and saliva on the mouthpiece 01 or tube 02 side move to the distal end device 04 side. There is an appliance to avoid (see FIG. 8, Patent Document 1).

However, this commercial product is not able to block the passage of gas and saliva well because the diaphragm 08 is a thin film made of synthetic resin or nonwoven fabric with fine open cells, so it is not enough to eradicate cross contamination. There is a lack of completeness. Further, since the diaphragm 08 is stretched flat and is provided in a tension state, it is stretched at the time of intake and exhaust, and itself has elastic resilience. However, if the air leaks, the sample sample 06 cannot be sucked into the tip tool 04 well, or the sample sample 06 cannot be discharged from the tip tool 04, which makes operation very difficult. Further, since the housing 07 is disposed immediately behind the attachment 03 of the tip tool 04, the housing 07 interferes with the operation especially when looking through a microscope or the like, and the delicate and delicate operation is hindered. And the handling was very inconvenient.

  The present invention was researched and developed in order to solve the above-mentioned problems of the prior art, and the purpose of the present invention is to make it very easy to collect and discharge a very small amount of sample sample, and at the same time, it is economical. In addition, the present invention is to provide an exhalation / inspiration pipette that can effectively prevent cross contamination.

  In order to achieve the above object, an exhalation / inspiration actuating pipette according to claim 1 of the present invention is configured such that a gas suction / exhaust drive source in a tip receiving a sample is a breath inhalation / exhaust. The suction mouth is detachably connected to the suction mouth mounting portion provided at one end of the airtight housing composed of a pair of cup bodies, while the tube is detachably connected to the tube mounting portion provided at the other end, In addition, the housing is provided with a gas-liquid impermeable diaphragm that isolates the internal space between the mouth side and the tube side, and a distal end tool for receiving the specimen sample is detachably connected to the distal end of the tube. The tip attachment member is detachably connected, and the housing is arranged so as to be biased toward the tip side as much as possible with respect to the tip attachment member.

According to this exhalation / inhalation operation pipette, when the suction port is held in the mouth and inhaled, the suction force sucks out the air in the space on the suction side in the housing and decompresses the space. With this decompression, the diaphragm is drawn into the space on the suction side. Due to the deviation of the diaphragm, the space on the suction side is opposed to the diaphragm, that is, the space on the tube side is decompressed. This reduced pressure is transmitted to the sample space of the tip tool through the tube, the sample space is decompressed, and the specimen sample is sucked into the sample space from the tip opening of the tip tool.
Conversely, in order to discharge the specimen sample to another location, when the tip is moved to a predetermined location and breath is blown into the suction port, the space on the suction side in the housing is pressurized. With this pressurization, the diaphragm is pushed into the space on the tube side. Due to the displacement of the diaphragm, the space on the tube side is pressurized, and this pressure is transmitted to the sample space of the tip tool through the tube, the sample space is pressurized, and the specimen sample is discharged from the tip opening of the tip tool. Is done.

  In the pipette for exhalation / inhalation operation configured as described above, there is only a tip attachment member on the distal end side of the tube, and only the tip insert inserted therein, and the housing is separated from the tip attachment member. It will be biased to the mouth side near the mouth as far away as possible. As a result, for example, when manipulating a sample while looking into a microscope, there is no housing that occupies a large portion of the overall volume of the pipette near the eyepiece and around the hand holding the tip. The degree of freedom of operation has been greatly improved, making it very easy to handle.

  In addition, since the housing is located near the mouthpiece at a distance from the tip, the sample space of the tip due to the movement of the diaphragm in the housing compared to the conventional product in which the housing is located immediately behind the tip. The pressure and pressure acting force applied to the inside is hardly affected directly, and is propagated through the air passage in the long tube to change to soft pressure and pressure acting force. Inhalation and discharge can be suppressed, and more delicate handling is possible.

In addition, the diaphragm in the housing isolates the ventilation path on the suction side from the ventilation path on the tube side and the tip side, so that air in contact with the specimen sample to be aspirated is sucked through the tube. Never reach the mouth. Conversely, exhaled air or saliva from the mouthpiece does not reach the tip through the tube. Therefore, cross contamination can be prevented well.

In the above configuration, according to the present invention, as described in claim 2, the diaphragm has an area larger than the plane area of the opening of the equator portion of the cup body and may not have elastic resilience itself. It is a thin film made of a flexible material, and the internal space of the housing can be displaced to the suction side and the tube side only by exhalation and inspiration. After the transition, the position is maintained as long as there is no next exhalation or inspiration Is desirable.
Unlike conventional ones, which generate elastic restoring force on the diaphragm itself, even if the suction port is removed from the mouth, the pressure inside the housing does not change at all, and the sample sample is inhaled and discharged from the tip. Or the risk of hindering quantitative sampling can be prevented.

According to a third aspect of the present invention, the housing is composed of a pair of cup bodies that are divided into the mouth side and the tube side at the equator portion, and the open portion of the equator portion is opposed to each other, and is provided at the equator portion. It is desirable that the connecting structures are integrally connected to each other, and the diaphragm is also integrally fixed at the connecting structure.
This is because the structure is simple and the manufacture is easy, and particularly when both cup bodies are connected only by fitting, even the user can easily exchange the diaphragm.

Further, according to a fourth aspect of the present invention, the connection structure portion includes a rib integrally extending toward the other cup body over the entire outer circumference of the outer wall of the equator portion of the one cup body, and the other cup body. An insertion groove which is provided with a dimension extending enough to contact the outer surface of the rib of the one cup body over the entire outer circumference of the outer wall of the equator part, and an opening toward the one cup body side is released between the outer wall and the outer wall. It is desirable that the ribs of one cup body be integrated together by inserting the ribs into the insertion groove of the other cup body together.
The means for fixing the diaphragm and the means for assembling the housing are simple, and the housing and the diaphragm can be easily assembled. Especially when both cup bodies are connected only by fitting, the user can also assemble the housing and the diaphragm. This is because disassembly and replacement are also facilitated. Moreover, as the ribs are inserted into the insertion grooves, the portions of the diaphragm that are addressed to the insertion grooves are both pushed into the insertion grooves and fixed, so that the insertion is performed. This is because the separated diaphragm exhibits a function as a sealing member, and keeps the insertion groove airtight, and hence the airtightness of the space in the housing.

Further, as described in claim 5, it is desirable that the outer shape of the housing is a sphere.
When the diaphragm is stretched, the tension applied to the diaphragm is concentrated on one part, such as a prism, and it is easier to be uniform over the entire circumference than when it is unbalanced over the entire circumference. This is because it is smooth and has an advantage that it is difficult to cause a local difference in atmospheric pressure change, and can be configured to be less bulky than a prism or cylinder, and can be easily handled and inexpensively provided.

FIG. 1 is an overall explanatory cross-sectional view including a partially enlarged view showing a pipette for exhalation / inhalation operation of the present invention, omitting an intermediate portion of a tube and a tip, and enlarging a part thereof; It is explanatory drawing of an effect | action. It is an expanded sectional view of the important section showing another composition of a mouthpiece. It is sectional drawing of the principal part which shows another connection means of a cup body. It is sectional drawing of the principal part which shows another connection means of a cup body. It is sectional drawing of the principal part which shows another structure of a front-end | tip tool attachment member. It is whole explanatory drawing of a conventional product. It is a whole explanatory drawing of the conventional product provided with the housing.

Embodiments of the present invention will be described below.
FIG. 1 is an overall view of an exhalation / inhalation operation pipette according to the present invention. This pipette 1 includes a mouthpiece 2, a housing 3 detachably connected to the mouthpiece 2, and a space S in the housing 3. , A tube 6 that detachably connects the housing 3 and the tip attachment member 5, and a tip 7 that is detachably connected to the tip attachment member 5. The distal end tool 7 includes a sample space 7A inside, and the specimen sample 8 (see FIG. 2) can be sucked and accommodated or discharged from the opening 7B at the distal end.

  The mouthpiece 2 includes a gripping portion 2A having a flat cross-sectional shape and a cylindrical connection portion 2B integrally connected to the lower end thereof so that the mouthpiece 2 can be easily gripped, and the connection portion 2B from the gripping portion 2A is provided inside. 2C is provided through the air passage.

  The housing 3 is composed of a pair of cup bodies divided into a cup body 9 on the mouth side and a cup body 10 on the tube side centering on the equator portion 3A, and the pair faces the open portion of the equator portion 3A. Are engaged with each other. Accordingly, the overall shape is spherical. In addition, a columnar connection portion 9A as a mouthpiece attachment portion is integrally provided at the top portion (pole portion) of the cup body 9 on the mouthpiece side, and the top portion (pole portion) of the cup body 10 on the tube side. In addition, a cylindrical connecting portion 10A as a tube mounting portion is integrally provided. Both of these connecting portions 9A and 10A are provided with air passages 9B and 10B connected to the internal spaces S1 and S2 of the cup bodies 9 and 10 at the axial center. The connection portion 9A of the cup body 9 on the suction mouth side and the connection portion 2B of the suction mouth 2 are connected to each other through a short tube 11 so as to allow ventilation. The short tube 11 is a flexible hollow body that can be elastically deformed and is made of silicone resin and has an air passage 11A therein.

Furthermore, the connection structure part 12 of both the cup bodies 9 and 10 and the said diaphragm 4 is provided in the equatorial part 3A of both the cup bodies 9 and 10. FIG. A specific configuration will be described in detail below.
First, on the side edge 9b of the outer wall 9a of the cup body 9 on the mouthpiece side, a rib 121 extending toward the tube side cup body 10 side over the entire circumference has a dimension equivalent to the wall thickness of the cup body 9. Projecting outward (radial direction), it is provided integrally. In addition, the entire circumference of the edge 10b of the outer wall 10a of the equatorial part 3A of the cup-side cup body 10 is in contact with the outer surface of the rib 121 from a portion slightly deviated from the edge 10b to the top (pole side). The U-shaped cross-section of the U-shaped letter with the rib 122 protruding integrally toward the cup body 9 side on the mouthpiece side and the opening opened toward the cup body 9 side on the mouthpiece side. An insertion groove 13 having a letter shape is formed. Therefore, the housing 3 is formed by inserting the rib 121 of the cup body on the mouthpiece side into the insertion groove 13 to a predetermined depth.

The diaphragm 4 is fixed at the edge of the equatorial part 3 </ b> A of the cup bodies 9, 10 of the housing 3. The diaphragm 4 has a flat area larger than the flat area of the open portion of the equatorial part 3A, and is composed of a flexible thin film sheet that does not have elastic resilience. The material is a thin film of silicone resin. The thickness of the sheet is appropriately selected depending on the collection amount of the specimen sample 8, but in the case of this embodiment, it is 0.04 to 0.12 mm, preferably 0.05.
The thing of appropriate thickness is employ | adopted from the range of -0.1mm.

  In order to mount the diaphragm 4 in the housing 3, the diaphragm 4 is placed on the open part of the equatorial part 3 </ b> A of the tube-side cup body 10. Next, the central portion is appropriately pushed into the space S2 of the tube-side cup body 10 until the central portion is close to the top (pole) of the cup body 10. As a result, as shown in FIG. 1, the diaphragm 4 is accommodated in a shape in which the central portion is greatly recessed into the space S2. Subsequently, the rib 121 of the cup body 9 on the mouthpiece side is put on the insertion groove 13 of the cup body 10 on the tube side. From this state, by pressing the cup bodies 9 and 10 closer to each other, the rib 121 of the cup body 9 on the mouthpiece side is inserted into the insertion groove 13 of the cup body 10 on the tube side. . Along with the fitting of the rib 121 to the insertion groove 13, both the portions of the diaphragm 4 that are addressed on the insertion groove 13 are pushed into the insertion groove 13 and fixed. . Since the width of the insertion groove 13 is narrow enough to insert the rib 121, the inserted diaphragm 4 functions as a seal member, and the insertion groove 13 is kept airtight well, The airtightness in the housing 3 is kept good.

  Accordingly, since the diaphragm 4 is a flexible thin film sheet that does not itself have elastic resilience, two spaces in the housing 3 formed by the diaphragm 4, that is, the mouth side space S 1 and the tube. It is possible to move freely between the side space S2 along with exhalation / inspiration, and after the transition, the position is maintained as long as there is no next exhalation or inspiration. Thus, unlike the conventional case in which an elastic restoring force is generated in the diaphragm 4 itself, even if the suction mouth 2 is removed from the mouth, there is no change in the atmospheric pressure in the housing 3, and the specimen sample 8 is attached to the tip tool 7. It is possible to prevent inhalation / exhaustion due to delusion and the possibility of disturbing quantitative collection.

  The tip attachment member 5 is a hollow cylindrical body integrally provided with an air passage 5A. A connection attachment 16 is detachably inserted into the opening 5B on the tube 6 side of the air passage 5A, with a part protruding toward the tube 6, and the other end of the tube 6 is detachably attached to the protruding portion 16A. It is fitted outside. Further, the other end of the tip attachment member 5 is formed in an insertion hole 5C that gradually widens, and the tip 7 is removably inserted into the insertion hole 5C. In this embodiment, the tip tool 7 is configured to be applicable to a small-diameter glass tube for a micropipette as shown in the figure. Further, since the tip attachment member 5 is made of an elastic and flexible material, for example, a silicone resin, the outer surface of the tip attachment 7 is not limited to a small diameter glass tube. Familiarize yourself and keep it airtight.

  The tube 6 is an elastically deformable and flexible hollow body having an air passage 6A therein. The length of the tube 6 employed in this embodiment is 30 cm. The length of the tube 6 is appropriately selected as necessary. The diameter of the air passage 6 </ b> A of the tube 6 is formed substantially equal to the outer diameter of the connecting portion 10 </ b> A provided in the tube side cup body 10 of the housing 3, and one end is the tube side cup body 10. Is externally fitted to the connecting portion 10A.

  In addition to preventing the cross contamination, the mouthpiece 2, the housing 3, the diaphragm 4, the tip attachment member 5, the tube 6, the tip 7, the short tube 11, the rib 12, and the connection attachment 16 From the characteristics of this type of pipette, which maintains the cleanliness of the specimen sample 8 and the reliability of data, etc., a synthetic resin material is desirable to prevent the plasticizer from evaporating like natural rubber. Specifically, polyvinyl chloride, silicone resin, polypropylene, polyethylene, etc. are employed.

In the present embodiment, in the state where the components shown in FIG. 1 are connected to each other, the mouthpiece 2 is held in the mouth and the breath is inhaled. This suction force sucks out air in the suction side space S1 in the housing 3 through the air passage 11A of the short tube 11, and decompresses the space S1. With this decompression, the diaphragm 4 is drawn from the position indicated by the imaginary line in the space S2 to the position indicated by the solid line on the space S1 side in FIG. By the transition of the diaphragm 4, the space S 1 on the suction side faces the diaphragm 4, that is, the space S 2 on the tube side is decompressed. This reduced pressure is transmitted to the sample space 7A of the tip tool 7 through the air passage 6A of the tube 6, the sample space 7A is reduced in pressure, and a specimen sample (culture liquid in the example) 8 is discharged from the tip opening 7B of the tip tool 7. It will be sucked into the sample space 7A.

  Conversely, in order to discharge the specimen sample 8 to another location, the tip 7 is moved to a predetermined location, and when the breath is blown into the mouthpiece 2 from the state shown in FIG. 4 is moved from the suction-side space S1 indicated by the solid line in FIG. 2 to the tube-side space S2 indicated by the solid line in FIG. The transition of the diaphragm 4 is performed by pressurizing the gas in the space S2 on the tube side from the ventilation path 6A of the tube 6 through the ventilation path 5A of the distal end tool mounting member 5 into the sample space 7A of the distal end tool 7. The specimen sample 8 stored in 7A is discharged from the opening 7B.

  In the present embodiment, the diaphragm 4 in the housing 3 separates the air passages 2A and 11A on the suction mouth 2 side from the air passages 6A and 7A on the tube 6 side and the distal end tool 7 side. Air in contact with the specimen sample 8 to be aspirated does not reach the mouthpiece 2 via the tube 6. Conversely, exhaled air or saliva from the mouthpiece 2 does not reach the tip 7 through the tube 6. Therefore, cross contamination can be prevented well.

  Moreover, as described above, the diaphragm 4 is a flexible thin film sheet that does not have elastic resilience as described above. Therefore, the space in the housing 3 is sucked into the housing 3 along with exhalation and inspiration. It is possible to freely move between the mouth-side space S1 and the tube-side space S2, and after the transition, the position is maintained as long as there is no next expiration or inspiration. Thus, unlike the conventional case in which an elastic restoring force is generated in the diaphragm 4 itself, even if the suction mouth 2 is removed from the mouth, the air pressure in the housing 3 is not changed, and the specimen sample 8 is removed from the tip 7. Against the danger of inhaling and discharging in a delusion and hindering quantitative sampling.

  Furthermore, only a small-diameter tip tool mounting member 5 and a tip tool 7 inserted into the tube 6 are present on the tip side of the tube 6, and the housing 3 is possible from the tip tool mounting member 5. It is biased to the mouthpiece 2 near the mouth far away. As a result, for example, in the operation of the specimen sample 8 while looking through a microscope, the housing 3 occupying a large portion of the bulk of the entire pipette does not exist near the eyepiece and around the hand gripping the tip tool 7. The degree of freedom of operation of the pipette 1 is remarkably improved and it is very easy to handle.

  Further, since the housing 3 is located near the mouthpiece 2 at a distance from the tip 7, the tip due to the transition of the diaphragm in the housing 3 is compared to the conventional product in which the housing is located immediately behind the tip. The pressure reducing and pressurizing force applied to the sample space 7A of the tool 7 is not directly affected, and is propagated through the air passage 6A in the long tube 6 so that the soft pressure reducing and pressurizing force is applied. It is possible to suppress instantaneous and sudden inhalation and excretion, and enable more delicate handling.

  In addition, although the example which connected both connection parts 2B and 9A with the short tube 11 was shown in the example of a figure, as shown in FIG. 3, the internal diameter of the connection part 2B of the suction mouth 2 is set to the connection part 9A of the said suction mouth side. A configuration in which the connection portion 2B of the suction mouth 2 is directly fitted into the connection portion 9A of the cup body 9 on the suction mouth side can be employed in the same manner as the outer diameter.

Further, when the housing 3 is configured, an adhesive (not shown) is appropriately poured into the insertion groove 13 as necessary, and the diaphragm 4 and the inner surface of the insertion groove 13 are adhered by this adhesive, It can be connected integrally or integrally by welding. This is an effective means for securing higher airtightness. In addition, as shown in FIG. 4, the stepped portion 14 formed on the connection structure portion 12 between the cup-side cup body 9 and the tube-side cup body 10 formed after the cup bodies 9 and 10 are fitted is bonded. A means for joining with the agent 15 can also be adopted.

  Further, as shown in FIG. 5, the housing 3 does not have the ribs 12, and both cup bodies 9 and 10 have the same diameter, and the edges 9 b and 10 b of the outer walls 9 a and 10 a of the equatorial part 3 </ b> A are In the state where the diaphragm 4 is sandwiched between them, it is also possible to adopt a means for directly connecting the diaphragms 4 together by means such as an adhesive (not shown) or heat welding.

In addition, a connection attachment 16 is employed for connecting the tip tool attaching member 5 and the tube 6, but instead of this structure, as shown in FIG. 6, the connecting tube portion 5 </ b> B is integrally formed with the tip tool attaching member 5. Can also be provided.
Specifically, the connecting cylinder portion 5B is integrally protruded from the upper end of the distal end tool attaching member 5 so that the outer diameter is slightly longer than the diameter of the air passage 6A of the tube 6. This is advantageous in that it can be provided at a lower cost with a reduced number of parts, and can be reduced in labor and manufacturing.

  Further, in the illustrated example, the insertion groove 13 is provided in the tube body 10 on the tube side, and the rib 121 of the 9 edge 9b of the cup body on the mouthpiece side is inserted into this. Even if the structure, that is, the structure in which the insertion groove 13 is provided in the cup body 9 on the mouthpiece side and the rib provided on the edge 10b of the cup body 10 on the tube side is inserted into this, the same effect can be obtained. Needless to say.

DESCRIPTION OF SYMBOLS 1 ... Pipette 2 ... Suction mouth 2C ... Air flow path 3 ... Housing 3A ... Equatorial part 4 ... Diaphragm 5 ... Tip attachment member 5A ... Air passage 6 ... Tube 6A ... Air passage 7 ... Tip tool 7A ... Sample space 8 ... Sample sample DESCRIPTION OF SYMBOLS 9 ... Suction side cup body 10 ... Tube side cup body 12 ... Connection structure part 121 ... Rib 13 ... Insertion groove S ... Space in housing S1 ... Space on suction side S2 ... Space on the tube side

JP 2010-156545 A

Claims (5)

  The suction / exhaust drive source of the gas in the tip that receives the sample is breath suction / exhaust, and the suction port is attached to / detached from the suction port attachment at one end of the airtight housing consisting of a pair of cups. While being connected freely, a tube is detachably connected to a tube mounting portion provided at the other end, and the housing is gas-liquid-impermeable so as to isolate the inner space from the mouth side and the tube side. A diaphragm is provided, and a tip tool mounting member for detachably connecting a tip tool for receiving the specimen sample is detachably connected to the tip of the tube, and the housing is connected to the tip tool mounting member. A pipette for exhalation / inhalation operation, wherein the pipette is arranged as biased as possible to the mouth side.   The diaphragm is a thin film made of a flexible material having an area larger than the plane area of the opening of the equator of the cup body and itself does not have elastic resilience. The pipette for exhalation / intake operation according to claim 1, wherein the pipette is configured so that the space can be displaced between the mouth side and the tube side, and after the transition, the position is maintained as long as there is no next intake / exhaust.   The housing is composed of a pair of cup bodies divided at the mouth side and the tube side at the equator part, facing the open part of the equator part, and being integrally connected to each other by a connection structure part provided on the equator part, The exhalation / inhalation operation pipette according to claim 1, wherein the diaphragm is also integrally fixed at the connection structure portion.   The connection structure includes a rib integrally extending toward the other cup body over the entire outer periphery of the equator outer wall of one cup body, and the one cup over the entire outer periphery of the equator outer wall of the other cup body. An insertion groove is provided that is extended to be in contact with the outer surface of the rib of the body, and an opening toward the one cup body is released between the outer wall and the outer wall. The pipette for exhalation / inhalation operation according to any one of the above items 1 to 3, which is integrated by being inserted into the insertion groove of the other cup body together with the diaphragm.   The pipe for expiratory / inspiratory operation according to any one of claims 1 to 4, wherein the outer shape of the housing is a sphere.

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