JP3179208B2 - Blood bag - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention provides a blood bag capable of separating blood components into components more efficiently when blood collected from a donor is separated and collected.

[Prior art and problems of the prior art]

[0002] A blood bag 51 described in Japanese Patent Publication No. 63-20144 has a blood collection bag 52 as shown in FIG.
Transfer tube 55 and lower transfer tube 55
The Bed 56 is provided, the upper transfer tube 55 and the lower transport Ju
Blood component separation bags 53 and 54 are attached to the other end of the probe 56 . 57 is a blood collection tube. Separation of blood components using these blood bags 51 has been performed as follows.

The blood collected in the blood bag 51 is separated into an upper layer, an intermediate layer, and a lower layer by centrifugal separation.
In the blood component separator described in No. -10229, the blood sample is mounted between two parallel pressing plates, and pressure is uniformly applied to the blood sample 52 from one side, so that only the highly viscous intermediate layer remains in the blood sample bag 52. As described above, the upper layer (plasma) was discharged from the upper outlet 59a of the blood collection bag 52, and the lower layer (red blood cell layer) was discharged from the lower outlet 59, and collected in the blood component separation bags 53 and 54, respectively.

However, from the lower outlet 59 of the blood collection bag 52, the blood component separation bag 5 of the lower transfer tube 56 is connected.
Since no means for closing the blood flow path is provided up to the end on the 4th side, when blood components are separated, blood enters the lower transfer tube 56 and lowers the red blood cell layer. Outlet 5
9, blood component separation bag 5 via lower layer transfer tube 56
At the time of collection into the sample No. 4, other blood components such as leukocytes may be mixed.

The intermediate layer (buffy coat) tends to adhere to the inner wall surface of the blood collection bag 52 and may remain in the lower layer. For this reason, the red blood cell layer may be collected in the blood component separation bag 54 from the lower outlet port 59 via the lower transfer tube 56 while containing the white blood cells, and it has been difficult to collect a pure red blood cell layer. The inventors of the present invention have conducted intensive studies to solve the above-described problems, and as a result, have arrived at the next invention.

[0006]

According to the present invention, blood collected in a blood collection bag is subjected to centrifugal separation to form an upper layer, an intermediate layer,
A blood bag used for separation into a lower layer, wherein an upper transfer tube 5 and a lower transfer tube are provided at the upper end of the blood collection bag 2.
Connect one end of the tube 6 and transfer the upper layer
At the other end of the tube 5 and the lower transfer tube 6
The upper layer separation bag 3 and the lower layer separation bag 4 are attached respectively,
End of the lower layer transfer tube 6 on the blood collection bag 2 side
Is extended to the lower part in the blood collection bag 2 and
The lower outlet 9 is fixed near the bottom of the blood collection bag 2.
And a blood bag 1 provided with a blood flow passage closing member 10 (20) near the upstream of the outlet 9 .

The present invention also relates to a blood bag used for separating blood collected in a blood collection bag into an upper layer, an intermediate layer, and a lower layer by centrifugation.
The upper transfer tube 25 and the lower transfer tube 2
6 and one end of the upper transfer tube.
To the other end of the lower transfer tube 26
Equipped with upper and lower separation bags 23 and 24 respectively
And, wherein an end portion of the blood collecting bag 22 of the lower transfer tube 26 is extended to the intermediate layer residence corresponding portions of the blood collecting bag 22, the suction bi coated with adsorbent leukocytes into the blood collecting bag 22 - encapsulating's A blood bag 21 is provided.

The present invention also relates to a blood bag used for separating blood collected in a blood collection bag into an upper layer, an intermediate layer, and a lower layer by centrifugation.
2 at the upper end , upper layer transfer tube 35 and lower layer transfer tube
36 and connected to the upper layer transfer tube.
And the other end of the lower transfer tube 36
Equipped with upper separation bag 33 and lower separation bag 34 respectively
Then , a partition 40 is formed in the blood collection bag 32, and the interior thereof is fractionated into a blood component storage chamber 42 and an outflow passage 41 below the blood component.
An outlet 39b is formed at the upper end of the lower passage 39 and the outflow passage 41 near the bottom, and near the upstream of the lower passage 39.
Providing blood bag 31 mounted closing member 10 (20 and 43) of the blood flow path near.

[0009]

The upper and lower layers of the intermediate layer can be recovered in a pure state without being mixed.

[0010]

FIG. 1 is a schematic view of a blood bag 1 according to the present invention. The blood bag 1 is moved to the upper end of the blood collection bag 2
Connect one end of feed tube 5 and lower transfer tube 6
The upper transfer tube 5 and the lower transfer tube.
The upper layer separation bag 3 and the lower
It is configured by mounting the layer separation bag 4.
End of the lower layer transfer tube 6 on the blood collection bag 2 side
Extends to the lower part in the blood collection bag 2 as shown in FIG.
And is fixed to the welding portion 15. Above said
A lower layer near the bottom of the blood collection bag 2 of the layer transfer tube 6
An outlet 9 is formed, and a closing member 10 for the blood flow passage is mounted near the upstream of the outlet 9 . The closure member 11 of the blood circulation path is found on the end of the blood collecting bag 2 side of the upper <br/> transfer tube 5.

The closing members 10 and 11 are composed of a large-diameter portion 13 and a small-diameter portion 14 via a thin-walled portion 12. The thin-walled portion 12 is easily broken by finger pressure, and the small-diameter portion 14 is removed. The road can be opened. In the present invention, a spherical closing member 20 can be used instead of the closing member 10 as shown in FIG. Closing member 20
Is made of plastic or the like (specific gravity of about 1.09) having a higher specific gravity than red blood cells (lower layer of blood components).
Is when centrifuged blood components are separated into an upper layer (plasma), an intermediate layer (buffy coat), and a lower layer (red blood cells)
It can be detached from the lower transfer tube 6 to open the blood passage.

Next, an example of a method of using the blood bag 1 of the present invention will be described. After blood is collected from a donor via the blood collection needle 8 and the blood collection tube 7 into the blood collection bag 2, the blood collection tube 7 is welded and cut, and centrifuged with the blood outlet of the blood collection bag 2 completely closed. The upper layer,
Separate into an intermediate layer and a lower layer. Subsequently, the closing members 10 and 11
Is broken to open the blood flow path of the upper transfer tube 5 and the lower transfer tube 6, and then presses the blood collection bag 2 to remove the upper layer (plasma) to the upper outlet 9a so that the intermediate layer remains in the blood collection bag 2. Then, the lower layer (red blood cells) is collected into the blood component separation bag 4 through the outlet 9 and the lower layer transfer tube 6 through the upper layer transfer tube 5 and the blood component separation bag 3.

FIG. 4 is a schematic view of another blood bag 21 of the present invention. The blood bag 21 is located on the blood collection bag 22 side.
The end of the lower transfer tube 26 is extended to a portion corresponding to the middle layer retention of the blood collection bag 22, and the blood collection bag 22 is further filled with adsorption beads 30 coated with a leukocyte adsorbent. The specific gravity of the adsorption beads 29 is set to the same level as that of the intermediate layer (buffy coat) of the blood component (the other components are substantially the same as those of the blood bag 1 in FIG. 1, so detailed description is omitted). .

Next, an example of a method of using the blood bag 21 shown in FIG. 4 will be described. As in the case of the blood bag 1, blood is collected and separated into an upper layer, an intermediate layer, and a lower layer. Adsorption beads 3
0 is floating at the same position as the intermediate layer and leukocytes are adsorbed beads 30
Is adsorbed. After the closing member 31 is broken to open the blood flow paths of the upper transfer tube 25 and the lower transfer tube 26, the blood collection bag 22 is pressed to press the intermediate layer and the intermediate layer (white blood cells).
The upper layer (plasma) is transferred to the blood component separation bag 23 via the upper transfer tube 25 and the lower layer (red blood cell layer) is transferred to the lower outlet 29 and the lower layer so that the adsorption beads 30 adsorbed on the blood collection bag 22 remain in the blood collection bag 22. The blood is collected into the blood component separation bag 24 via the transfer tube 26. Even if white blood cells are mixed in the red blood cell layer, the white blood cells are adsorbed by the adsorption beads 30, so that the red blood cells can be collected into the blood component separation bag 24 via the outlet 29a in a pure state.

FIG. 5 is a schematic view of another blood bag 31 of the present invention. The blood bag 31 has a partitioning portion 40 formed by welding or the like to the blood collection bag 32, and the inside thereof is divided into a blood component storage chamber 42 and a blood component lower outflow channel 41. Near the bottom of the blood collection bag 32 of the partition section 40
A lower layer passage 39 is formed beside the passage 39, and close-up members 10 and 20 similar to FIGS. 2 and 3 are mounted near the upstream of the passage 39 as shown in FIGS. Further, the outflow channel 41
A lower outlet 39b is formed at the upper end of the
A lower layer transfer tube 36 is connected, and a blood component separation bag 34 is connected to the distal end of the tube (this is substantially the same as the blood bag 1 of FIG. 1 and will not be described in detail).

At the time of use, as in the case of the blood bag of FIG. 1, after separating into an upper layer, an intermediate layer, and a lower layer by centrifugation in the blood component storage chamber 42, the closing member 10,
11, the blood flow path is opened, the blood collection bag 32 is pressed, and the upper layer (plasma) is passed through the upper outlet 39a and the upper layer transfer tube 35 so that the intermediate layer remains in the blood collection bag 32. Lower layer (red blood cells) to blood component separation bag 33
Is collected into the blood component separation bag 34 via the passage 39, the outflow passage 41, the outlet 39b, and the lower transfer tube 36.

As another embodiment of the closing member arranged upstream of the lower passage 39, a closing member 43 as shown in FIG. 8 can be arranged. Closing member 43
Is composed of a rupture portion 44 and a ruptured portion 45. The rupture portion 44 has a protruding portion 49 formed at an upper portion, and a lower portion is fixed to a bottom portion of the blood collection bag 32. On the other hand, the to-be-fractured portion 45 has a film portion 48 formed inside the cylindrical body 46 with a thin portion 47 interposed therebetween, and the to-be-fractured portion 45 is arranged near the upstream of the passage 39. The rupture portion 44 and the rupture portion 45 are arranged close to each other with a slight gap left therebetween, and are pushed in the direction of the arrow to form the film portion 4 by the projection 49.
The blood passage can be opened by detaching the tube 8 from the tube body 46 or by twisting the ruptured portion 45 back and forth and left and right to detach the membrane portion 48 from the tube body 46.

[0018]

As described above, according to the present invention, the upper layer and the lower layer can be separated and discharged before and after centrifuging the blood components while maintaining a state where the blood components are not mixed with each other. A pure blood component free of components can be obtained.

[Brief description of the drawings]

FIG. 1 is a schematic diagram of a blood bag of the present invention.

FIG. 2 is a partially enlarged view of FIG. 1;

FIG. 3 is a schematic view showing another embodiment of FIG. 2;

FIG. 4 is a schematic view of a blood bag of the present invention.

FIG. 5 is a schematic view of a blood bag of the present invention.

FIG. 6 is a partially enlarged view of FIG. 5;

FIG. 7 is a schematic view showing another embodiment of FIG. 6;

FIG. 8 is a schematic diagram showing another embodiment of FIG. 6;

FIG. 9 is a schematic view of a conventional blood bag.

[Explanation of symbols]

1, 21, 31 Blood bag 2, 22, 32 Blood collection bag 3, 23, 33 Blood component separation bag 4, 24, 34 Blood component separation bag 5, 25, 35 Upper transfer tube 6, 26, 36 Lower transfer tube 7, 27, 37 Blood collection tube 8, 28, 38 Blood collection needle 9, 19, 29 Lower outlet 9a, 19a, 29a, 39a, 39b Upper outlet 10 Closing member 11, 31 Closing member 12 Thin portion 13 Large diameter portion 14 Small diameter portion DESCRIPTION OF SYMBOLS 15 Fused part 20 Closure member 30 Adsorption beads 39 Passage 40 Partition part 41 Outflow path 42 Blood component storage chamber

────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-6-30986 (JP, A) JP-A-5-76581 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61J 1/10

Claims (3)

(57) [Claims] 1. A blood bag used for separating blood collected in a blood collection bag into an upper layer, an intermediate layer, and a lower layer by centrifugation, wherein an upper layer transfer tube 5 is provided at an upper end of the blood collection bag 2. Lower layer transfer
Connect one end of the tube 6 and transfer the upper layer.
At the other end of the feed tube 5 and the lower transfer tube 6
Equipped with upper separation bag 3 and lower separation bag 4 respectively
Then, the end of the lower layer transfer tube 6 on the side of the blood collection bag 2 is
It extends to the lower part in the blood collection bag 2 and is fixed to the welding portion 15.
And a lower outlet 9 near the bottom of the blood collection bag 2.
A blood bag 1 formed and provided with a blood flow passage closing member 10 (20) near the upstream of the outlet 9 . (2)Blood collectionThe blood collected in the bag is centrifuged.
Used to separate the upper, middle and lower layers
A blood bag,, Upper layer transfer tube 25 and lower layer
Connect one end of the transfer tube 26 and
Other than the upper transfer tube 25 and the lower transfer tube 26
The upper layer separation bag 23 and the lower layer separation bag
, And wear it,  SaidUnderlayerTransfer tube 26Blood collection bag 22 sideThe end of
The blood collection bag 22 is extended to a position corresponding to the intermediate layer stagnation.
A blood bag 21 containing beads. (3)Blood collectionThe blood collected in the bag is centrifuged.
Used to separate the upper, middle and lower layers
Blood bag, which is located at the upper end of the blood collection bag 32., Upper layer transfer tube 35 and lower layer
Connect one end of the transfer tube 36 and
In addition to the upper transfer tube 35 and the lower transfer tube 36
The upper layer separation bag 33 and the lower layer separation bag
With the power 34  A partition 40 is formed in the blood collection bag 32,
The blood component storage chamber 42 and the blood component lower outflow channel 41
The blood collection bag 32 of the partition 40Near bottomLower tier
An outlet 39b is formed at the upper end of the passage 39 and the outflow passage 41.
And the lower channel 39Upstream neighborhoodBlood flow passage closing member
10 (20, 43).
Egg 31.