JP3178460U - Photosynthesis unit device - Google Patents

Abstract

A photosynthetic unit device for efficiently culturing cyanobacteria is provided.
In a photosynthetic unit device, a three-stage culture cell made of a transparent glass container is fixed by a frame structure. The culture cell includes a large-diameter cylindrical portion 1a having a branch pipe for overflow of the culture medium and an upper ridge, a spherical cylindrical portion 1b, and a small-diameter cylindrical protrusion 1c connected to the bottom of the spherical body. The culture cells are vertically arranged in three stages, with the upper, middle, and lower culture cells 1 to 3 on the center line. The cyanobacteria grown in the culture cell flows down from the upper stage to the middle stage and from the middle stage to the lower stage by opening a movable valve provided at the lower end of the protrusion 1c.
[Selection] Figure 2

Description

  Microbiological cyanobacteria in the hydrosphere have a stable and efficient system for converting photon energy into molecular orbitals. A small and compact reduced-type culture cell unit apparatus is constructed that can induce microbiota containing cyanobacteria to the maximum growth rate under optimum conditions and maintain the photosynthetic performance while maintaining it.

  Cyanobacteria (organic substances) grown and grown from inorganic substances and light energy can be easily separated and harvested for use in foods, and the use of biotechnology oxidative fermentation technology to further increase the utility value while reducing and oxidizing each other. A technique for establishing a conjugated system is also possible.

  When looking at the background technology, in this space-time field, most of the conventional technology is due to the Ergon reaction (Gibbs energy is the positive reaction direction) which is a vector of self-preservation, self-replication, and self-advantage. Yes, always accompanied by free energy consumption (oxidation / divergence). Humans have expanded and developed with oxidation-type technology, but they are still immersed in this trend of technology. However, there are field limitations, and the physical energy constraints of this space-time field are obeyed. Until now, only the oxidation-type technology has been expected to limit the resources, food, population, etc., and conversely manifested as environmental pollution. In the conventional oxidation type technology, energy declines also in the soil industry, which is a general industry and agricultural base. Currently, there are some reduction-type technologies, but it is hard to say that they are drawing rapid progress in comprehensive science and technology. In contrast, culture of oxygen-generating cyanobacteria can absorb the photon energy emitted by the sun with a photosynthetic dye and convert it into molecular orbitals. First, a reduction technology that acquires such free energy is necessary.

  JP 2001-231538 A   JP-A-10-108565   JP 2003-222 A   JP2003-24043   JP2005-40035

As a background art of the reduction technique, there is an invention of the above-mentioned patent application. Differences between Patent Documents 1 to 5 and those similar to the present invention will be described.
In (Patent Document 1), a mechanism that separates alga bodies by a fixed plate with a through slit separating the upper tank in which the light dispersion carrier is disposed and the lower tank without the light dispersion carrier, and a movable plate that can be opened and closed, and a spurger The culture solution is mixed and stirred by aeration, and the algal bodies are naturally settled in the lower tank by a downward flow to separate the algal bodies.
Since the present invention is a fluid culture and always circulates in the cell, the light and dark portions of the upper and lower tanks are not necessary. Furthermore, algal bodies are gathered in the lower center of the cell hydrodynamically by vortex flow (lateral circulation flow) generated by the axial flow. Further, in the three-stage cell, in order to prevent the algal body concentration and the criticality of the algal body concentration, the upper and middle cells are caused to flow down to the lower cell by the connected drain. The separation mechanism (slit) by the moving plate in the horizontal direction is not used, but it is concentrated and concentrated in the cylinder at the bottom of the lower cell. For this reason, a slit and a liquid feed pump are unnecessary.
(Patent Document 2) has a light source for internal irradiation installed in a culture tank, the alga body separation part at the bottom of the tank has a funnel shape, and the culture solution agitation in the culture part and the alga body separation at the alga body separation part under the tank Is controlled by adjusting the flow rate of the aeration gas.
In this case, fluid culture by convection cannot be performed. The present invention performs fluid culture in a spherical cell. Mitochondrial growth by photosynthesis is convection (longitudinal circulation flow), and aggregate concentration of algal bodies is performed by vortex. In addition, the photon is significant for obtaining energy from the outside, that is, from the sun as much as possible by external irradiation of sunlight and artificial light. In addition, the gas replacement blade and fluid screw ensure the speed-control of photosynthesis.
(Patent Document 3) is characterized by a disc turbine blade for increasing the dissolved oxygen concentration in the culture tank. In the present invention, cyanobacteria are oxygen-generating reductive bacteria. Is unnecessary.
(Patent Document 4) is characterized in that a charged chemical solution and compressed air are charged into a stirring blade from the tip of a nose. In the present invention, inorganic salts are injected by an accurate quantitative drip injection method and gas replacement. Then, vertical convection in a vertical direction is caused to flow circumferentially in a spherical cell by a perforated spoon blade and an axial screw, and this is introduced into fluid culture by this convection.
(Patent Document 5) features a forced overflow by a head tank and a pump and a sealed horizontal culture tank. In the present invention, the culture cell is spherical and open.
The overflow is an overflow for maintaining the reference horizontal plane of the cell. The head tank is a tank for replenishment liquid fixed amount instillation.
In addition to the above-mentioned patent documents, if the acquisition and fixation probability of photon energy is the most important, the present invention is based on whether the acquisition and fixation of external energy (sunlight) is only in industrial production or consumer life. First, we recognized that it was necessary as a basic base of energy, and devised a device with understanding.

In order to preserve the photon energy from the solar background radiation on the earth, we must rely on the photosynthesis of reduced organisms.
The cyanobacterial dye multi-channel optical system and the corresponding synthesis system have excellent conversion efficiency to this molecular orbital. In order to maintain high-speed division with high efficiency under the optimum conditions for the growth and growth of cyanobacteria, the optical cell and biochemically stable culture cell and the device that can improve the yield of photons and the effect are small in size. It should be intended to be compact and unitized so that it can be installed not only on a plane but also spatially and three-dimensionally.

  To solve this problem, basic physics, chemistry, biological theory and knowledge are necessary, and at present, in order to adapt to human activities of food, energy, environment, and population problems, In addition to agriculture, there is a need for a unit device that can be universally arranged by regarding each individual, home plane, building-shaped space, and solid as a tree shape.

  When energy is viewed from the direction of a physical concept, life can be positioned as a kind of energy engine. Therefore, the acquisition of this energy becomes a necessary condition. In order to preserve and propagate the cyanobacterial photosynthetic system as a means for this purpose, we intensively devised and devised technologies.

  As one of the practical applications, the cyanobacteria are fluidly cultured in a transparent spherical glass (wire netting glass) cell structure. The capacity is 20L-30L, and this is regarded as one large leaf of a plant, and a photosynthesis reaction is performed. This culture cell is made up of upper, middle, and lower three-stage units. Fluid culture is performed by quantitative instillation method, rotary gas-liquid mixing, and convection (longitudinal circulation flow) using an axial screw. The concentration of cyanobacteria is increased by opening the upper and middle coaxially-coupled movable valves that have been concentrated and concentrated into a small-diameter cylindrical part by circulating flow) and flowing down a predetermined amount from the upper stage to the middle stage and from the middle stage to the lower stage. A switching cylinder valve is provided at the lower end of the lowermost cell, and the cylinder concentrates and concentrates the cyanobacteria in a small diameter cylinder. Opened by switching, pumped by plunger driving force, received and harvested by cup set with mesh. A control mechanism that maintains the optimal growth concentration value due to these structures and effects in equilibrium and this control unit were equipped.

  When the photosynthetic system is viewed as the probability (efficiency) of fixing the photon energy to the molecular orbitals, it manifests itself as the division rate and the number of growth in cyanobacteria of single-celled organisms and microalgae.

  The convection and vortex flow, which is the flow of the culture solution, in the transparent spherical cell is not a simple stirring flow, but the light and counter-irradiation, and the ecology of cyanobacteria, especially Spirulina, is floating. For details of the photosynthetic action, physics of thylakoid membrane electron transfer mechanism and stroma biochemical synthesis system, starch synthesis and cytoplasmic degradation synthesis system (glycolytic system and multiple synthesis pathways) Transparent sphere cell structure and fluid dynamics utilizing the fluidity of the culture solution adapted to biochemical action and circadian rhythm, synergistic effect by gas replacement by two-stage rotor and nutrient supply, and the photosynthetic action of cyanobacteria The effect of inducing maximum growth and increasing productivity efficiency appeared.

Molecules that are easier to use if the quantum energy, water (hereinafter referred to as H ₂ O), carbon dioxide (hereinafter referred to as CO ₂ ), inorganic salts, and the conditions of the field as the photosynthetic environment are met and energy is acquired. If it is converted to synthesize, it will be converted to organic fertilizer, ethanol, and chemical products using biotechnologies as well as food. And the effect of self-sufficiency and self-preservation mental stability by executing photosynthesis.

    Hereinafter, the form for implementing this invention is demonstrated based on FIGS.

Among cyanobacteria, Spirulina platensis (Spirulina) (NIES-39) (IAM M-135) is cultured. The inorganic salt nutrient solution composition is an SOT medium mainly composed of sodium bicarbonate (NaHCO ₃ ) and has a strong alkaline pH of 9.2 to 10.0 and can prevent foreign contamination such as contamination. Since the absorption spectrum of the photosynthetic dye is visible light of about 380 nm to 770 nm and has a wide absorption spectrum, it can be cultured under sunlight having a high light absorption efficiency, in a bright room, or an artificial light source such as a fluorescent lamp.

  The structure of the culture tank (cell) in which this reducing / oxygen-generating microorganism is cultured in a thin-walled transparent container (with a wire mesh glass or a reinforcing rib on the outside) so as not to interfere with the light transmittance of 20L to 30L. A large-diameter cylindrical part 1a with an upper flange with a branch pipe 1d (FIG. 1) for maintaining the liquid level of the culture liquid, a spherical body part 1b, and a small-diameter cylindrical projection 1c are connected to the center line on the center line. Thus, a transparent and spherical culture cell structure (FIG. 1) is formed in which the inner surface of the body portion requires a curved surface.

This culture cell is composed of a rack 29 (FIG. 2), which is a structure of four struts, in three stages, upper, middle and lower, and the upper cell 1, the middle cell 2 and the lower cell 3 are convection A (FIG. 1, on a spherical surface). The vertical circulation flow of the circumferential flow in the culture tank along the upper and lower sides is explained as convection, and the vortex flow B (FIG. 1, the lateral circulation flow in the culture tank along the spherical surface is explained as the vortex flow. ) Gently, and the upper, middle, and lower stages are cultured and grown by convection by convection, and concentrated and concentrated by vortex. The movable cell 4 is opened by the solenoid 26 with the cyanobacteria of the upper cell collected and concentrated in the small-diameter cylindrical portion 1c, and the cylindrical portion capacity is allowed to flow down to the middle cell by a predetermined amount.
The culture cell is basically composed of upper, middle, and lower three-stage units, and the number of upper cells can be freely increased or decreased.

In this way, the concentration of the middle stage cyanobacteria is increased. Similarly, a notch plunger valve 6b, 6c (FIG. 2) that can be switched by rotating 180 ° is provided at the lower end of the cell at the lower stage, and the cylinder is opened so that cyanobacteria have a small diameter. Assembling and concentrating the concentrated product in the cylindrical part of the tube to the cylinder 6a (FIG. 2), rotating the notched plunger 6b as a movable valve by 180 ° to close the upper culture cell side, It is driven to flow downward, or pumped by a plunger, and received and harvested by a cup 7 with a mesh set.
The notched plunger valves 6b and 6c can be linked together to open and close the cylinder, and suction and pressure feeding by the plunger drive can be operated manually or automatically.

Collective concentration and separation of cyanobacteria by vortex flow.
The optical system II of cyanobacteria electrolyzes water (H ₂ O) by light irradiation to separate oxygen (hereinafter referred to as O ₂ ) gas and moves it from the inside of the cell to the outside of the cell. During this time, buoyancy is generated by O ₂ gas, and it rises toward the water surface. When O ₂ gas bubbles are released, it sinks to the bottom of the water. In Spirulina, the buoyancy of about 0.02 to 0.04 m / s is slightly different depending on each individual. Move at speed. In the absence of light (at night), it remains at the bottom of the water due to the metabolic system and cell division activity.
The flow pattern and flow rate of the flow culture in the cell adapt to this ecological rhythm. In photosynthesis by light irradiation, it flows by gentle convection (flow rate 0.02-0.04 m / s), and when it is stationary on the bottom of the water without light irradiation, vortex flow (outer cell flow velocity is about 0.6 m / s or less) To collect and concentrate.

When the circumference in the cell by flow culture is about 1.2 m and the flow rate is 0.03 m / s, the circumference in the cell is about 40 seconds, the light irradiation side time is about 20 seconds, and the counter irradiation side time is about 20 seconds. Become.
In automatic control, the screw shaft is mounted in advance on the motor base at an angle of about 15 ° (Fig. 3) with respect to the cell axis, and the inclination of the screw shaft twist angle during convection (culture) is vertical and rotates. Number is forward rotation (rotation direction in Fig. 3) is about 15rpm and convection is performed. During vortex (nighttime), the inclination of the screw shaft twist angle is about 30 ° and a fulcrum is provided on the motor mounting base, and the solenoid 30 (Fig. 3) forms the inclination. The vortex is generated at a rotation speed of about 200 rpm, stopped when the vortex has spread over the entire culture cell, and concentrated and concentrated for 4 hours on standby (stop). By repeating this interval driving three times, it is caused to flow down from the upper stage to the middle stage and from the middle stage to the lower stage. The bottom separation is about 50cc at a time, when there is no light irradiation, or at night every 4 hours, twice a day, about 100cc per day by cylinder and plunger, moved to a mesh cup . The cyanobacteria separated into solid and liquid by the mesh cup are pasty. The product (paste-like) is obtained by washing / washing this with a mesh cup and draining it.

While injecting air and CO ₂ gas into the culture solution and dissolving it, O ₂ gas generated by the photosynthetic carbon dioxide assimilation is extracted from the culture solution, which is the inactivation of various enzymes due to oxygen sensitivity in the case of reducing microorganisms. This is done to avoid inhibition of crystallization. At the same time, a new inorganic salt nutrient solution is replenished by installing a stainless steel head tank 10 (FIG. 3) on the upper surface of the unit base, stocking the inorganic salt replenisher, and using a flow rate adjusting valve 11 such as a needle valve and a tube pipe 12. A quantitative drip infusion method is performed in which each drop is dropped into the transparent glass connecting tube 13 (FIG. 3), and each drop is contact-melted with air and CO ₂ gas in the tube.

The rotary gas-liquid mixing with the function to supply CO ₂ and inorganic salts to all the bacteria in the spherical cell evenly, the gas replacement blade 17 and the screw 18 that can maintain the predetermined flow form of each cell on the same axis The upper position is a culture cell equipped with gas-liquid mixing and gas replacement perforated spoon blades 17 and the lower position is a downward axial flow generating screw 18 and its apparatus.

The center of the spoon blade is cut into a U-shape so that the replenisher can be supplied to the center of the rotary punch spoon blade 17, and the fixed-dose teflon tube 14 inserted from the top does not come into contact. The structure is capable of rotating motion. By rotating the rotating direction of the spoon in the direction of travel on the concave side (the rotating direction in FIG. 3), the culture pressure is included to increase the internal pressure on the concave side and the external pressure on the back side (convex side) to be reduced. Through the flow holes processed into the liquid, the liquid flows through at an accelerated flow rate. This through flow creates a turbulent flow behind the spoon, and CO ₂ gas is mixed into the culture solution by the pressurizing effect on the front side (concave surface) of the perforated spoon blade. O ₂ gas is degassed from the culture solution by the pressure reducing effect on the back side (convex surface side) of the spoon from the culture solution, and finally gas is replaced by release into the atmosphere.

  The drip replenisher can be mixed into the culture medium in the cell by the turbulent flow behind the back side (convex surface) of the spoon. Due to the effect of the spoon blade, the new nutrient solution and the culture fluid mixed immediately move to the axial flow of the screw installed coaxially adjacent to the lower portion of the spoon blade, and supplied to the fluid culture in the cell. It becomes convection.

  The inorganic salt replenisher (nutrient solution, liquid medium) in the head tank 10 (Fig. 3) supplements each cell with nutrient solution depletion due to photosynthesis and divergence of the upper, middle and lower cells by quantitative infusion. Keep liquid volume constant. When the fluctuation amount of the culture solution in each cell exceeds the upper limit of a certain volume, the upper branch cell 1d (FIG. 1) and the overflow hose 8 (FIG. 2) are installed at the predetermined position of the cell. The middle cell is caused to flow down from the middle cell to the lower cell, and the overflow liquid in the lower cell is stocked in the overflow tank 9 (FIG. 2).

The culture medium in the cell is provided with a gas-liquid mixed gas replacement blade driven by a motor and a screw, and is mixed with supplementary nutrient solution, CO ₂ gas, and air C (FIG. 3) from the connection pipe 13 (FIG. 3). In addition, the upper and lower circumferential flows along the spherical surface are circulated and flowed as convection A (FIG. 1) and the lateral circumferential flow as vortex B (FIG. 1).

  With the automatic control of the control box 28 (FIG. 2), these flows are made gently, and light is absorbed and light intensity is adjusted while the culture solution is convectively flowed into the spherical cells 1a and 1b, and inorganic salts are added, gas is replaced and cultured. In order to maintain the constant temperature of the liquid (20 ° C. to 40 ° C.), the maximum culture growth rate is maintained at 20 ° C. to 35 ° C. under the optimum conditions maintained by the film heater 24 (FIG. 3) and the air conditioner 25 (FIG. 4). . In addition, the control of the rotational speed of the coaxial rotation of the perforated spoon blade and the axial screw and the ON / OFF interval change the flow velocity of the vortex by the control box 28 (FIG. 2).

  During culture division growth, the bacterial plastid antenna is moved to the light irradiation surface of the cell by convection A (FIG. 1), and the photon is absorbed by increasing the photon yield. Then, the weak light-receiving surface opposite to the light-receiving surface of the cell is moved along the spherical surface during the synthesis system action. This is done in order according to the circadian rhythm.

  Since the inner surface of the bottom of the cell is a spherical surface, the bacteria that have proliferated and divided according to the flow velocity are submerged and moved along the spherical surface by the fluid dynamics application of the vortex B (FIG. 1) of the culture solution. The action is generated, and finally, it is concentrated in the small-diameter cylindrical portion 1c at the lower end portion on the center line. For the concentrated culture fluid drain, a movable valve 4 in which a middle cell is connected on the same axis 5 (FIG. 2) can be provided and operated at an intended time interval. This is also to prevent reaching the critical concentration of mitotic cyanobacteria.

Grounds and advantages of being a transparent spherical glass cell.
In the case of a transparent and spherical glass cell, the reflected light can be used by using a reflecting plate 27 (a mirror or the like) in a part of which the photon can be incident on the entire spherical surface. In the case of hemispherical light irradiation, the location area on the light irradiation side (front side) of the cell is the photosynthetic carbon dioxide assimilation (light reaction) side, and the location area on the counter-irradiation side (back side) is metabolic nitrogen. It is the assimilation (dark reaction) side.
The front and back side area regions of the transparent spherical glass cell are divided, and by the flow of the culture solution by convection, the place can be changed and circulated by the unidirectional flow movement of the front and back place areas.

  The regulation of the assimilation balance between carbon assimilation by photosynthesis and nitrogen assimilation by metabolism is one of the factors that determine the division rate and growth rate. The convection flow rate can secure the residence time and travel time of each region, and by adjusting the flow rate, both assimilation balance and maintenance can be adjusted, and the absorption efficiency of photon energy and the productivity of anabolic substances can be improved.

  In terms of the absorption efficiency of photon energy, it is advantageous that the light intensity is higher in the optical system, but this is not necessary in the synthesis system, and continuous intense irradiation for a long time generates light damage and stress. The convection that is caused to flow circumferentially can prevent and prevent this light inhibition.

The advantage that the culture vessel (cell) can be cultured in a transparent spherical glass cell is that all cyanobacteria in the culture vessel are capable of convection in the cell by an axial screw of the culture solution. Since the flow velocity of the fluid is different and the overall flow order as a laminar flow also has a relative flow velocity difference in a fine region, the rotation (spinning) force acts on each cyanobacteria, and the rotation (spinning) To do.
Moreover, in a large convection, there is a vertical circumferential rotation (revolution) that circulates. By the rotation and revolution described above, the photochromic antennas of cyanobacteria can be evenly distributed with the photon acquisition probability, and the photosynthesis proceeds to maintain a uniform concentration distribution of cyanobacteria throughout the cell.

Π electron cloud antenna by molecular orbitals.
The photon is in midsummer and is incident on the light receiving area of 1 m ^{2 at} a wavelength of about 380 to 770 nm (visible light) 10 ²¹ per second. The photosynthetic pigments possessed by cyanobacteria for efficiently receiving these are wavelength body band channels of chlorophyll (a, b, d), carotenoids (carotene, xanthophylls) and phycobilins (phycocyanin, phycoerythin), which are carbon conjugates. Because it is a double bond, the antenna directivity of the π-electron cloud is generated. The photon yield of cyanobacteria depends on the light-receiving mechanism on this antenna side and the existence probability of electrons. Only a few have been earned. As a measure for improving the photon yield, in the present invention, the photon quantum antenna is rotated by varying the angle of the π-electron cloud antenna with respect to the photon incident optical axis by rotation and revolution of each individual cyanobacteria by convection in fluid culture. Can increase the probability of electron resonance (electron spin resonance / quantum yield) in the π-electron cloud, and also increase the probability of molecular orbital transition (molecular orbital conversion). That is, the rate at which the molecule is changed from the ground state to the excited state is increased, and acquisition and storage of energy (ATP, NADPH) are also increased. Energy diversion and oxidation always provide energy as needed, that is, a number of synthetic circuits and nucleotide synthesis pathways by the bacterial metabolic system and the progress of the ergonomic reaction are donated to the DNA self-replicating promoter, Division and proliferation are induced, and the target photosynthetic product is increased.

The rotation and revolution of cyanobacteria caused by the flow of the culture solution is a necessary condition for the division and growth of cyanobacteria by photosynthesis. Nutrient salts and CO ₂ gas in contact with the cell membrane of cyanobacteria are used by photosynthesis and lack and deficiency Therefore, it is necessary to move and remove the culture solution in this part, smoothly supply necessary substances and replace the gas with a new replenishment solution, and the flow of the culture solution performs this. In particular, the O ₂ gas generated by photosynthesis is quickly removed from the cell membrane surface, so that the oxidation reaction by active oxygen can be avoided in the reaction group of carboxylase and reduction / regeneration. Meet and maintain growth requirements.

The reason why the culture vessel (cell) is transparent and spherical glass.
The spectrum of solar photon energy is about 300 to 3000 nm on the surface of the earth, and the optical systems II and I with cyanobacterial plastids of microalgae in the hydrosphere are visible light having an absorption spectrum of about 380 to 770 (nm). The material must be capable of transmitting visible light. Moreover, since the culture medium in the cell is alkaline with a pH of 9.2 to 10, glass having chemical resistance is optimal. From the relationship of the strength of the glass and the flow and circulation of the culture solution, a spherical shape is suitable for the cell inner surface.

The reason why the cell diameter is about 0.4 m.
As the bacterial density and concentration in the culture medium increase, the photons are absorbed and the depth of arrival of the photons decreases, which limits the distance from the surface of the culture tank to the center. Therefore, a radius of about 0.2 m was set as a limit.

Reason why the spherical cell capacity is 20L-30L.
When the latitude is 30 degrees or more, the light intensity is halved and the air temperature (room temperature) is likely to fluctuate, so that the temperature of the culture medium in the cell also depends on this. The volume was about 25 L due to the adjustment of the concentration just before the bacteria critical, the depth of arrival of photons from 0.1 m to 0.15 m, and the temperature of the culture solution from about 20 ° C. to 40 ° C.

Grounds for three-stage culture tanks.
・ If there are more than three stages, it will be difficult to adjust the axis of the connecting shaft of the drain valve.
-If the height is 3m or higher, the height will be large, and if it is installed near a window, the lighting from the window will be poor.
・ In three or more stages, if the unit height is large, the center of gravity increases and becomes unstable.
・ In three or more stages, the replenisher head tank is in a high position, making management difficult.

  An apparatus unit in which a culture cell having such a structure and function is compactly set in a small space is suitable for a veranda and a window side 19 having incident light outside and inside the home 19 (FIGS. 4 and 5) of a general household. Install it in a half-day place.

  Even in the case of mass production of photosynthetic products in the hydrosphere, if this small cell unit is arranged in a spatial configuration in consideration of photosynthesis in a building type (apartment etc.) 21, it will be the same tree shape as plants (trees etc.), and photosynthesis production Sex goes up further.

  By operating the culture apparatus unit (FIGS. 2, 5, and 6) by a general household, an organic substance is automatically produced from the photon energy and the inorganic substance, and cyanobacteria can be harvested in-house.

  In the energy storage system, first, a part of the energy that is produced and fixed as an organic substance (biological substance) based on sugar, which is a CC chain, is produced by a reducing bioreactor (Cyanobacteria) 19 that is the conversion of photon energy. And part of it is put into the oxidation bioreactor 20 and converted into heat, energy (ethanol, etc.), organic fertilizer, and other useful substances through fermentation processes using biotechnology, glycolysis and many synthetic pathways. Can be converted (FIG. 6).

  In addition, when the reduction type and oxidation type bioreactor connection system is used as a mutual conjugate system and lives in the greenhouse 21 (FIG. 4), the system becomes a biosphere-like system, and the solar power generation 22 that supplements the mutual conjugate system is installed on the roof of the greenhouse. If a sufficient amount of space is provided to allow the photons to reach the cell moderately, the optical reaction system of cyanobacteria by direct sunlight and long exposure is controlled by the speed of the synthesis system. In order to prevent irradiation inhibition, the roof and walls of the greenhouse will be installed so that photons can enter properly.

  Controls that require power sources inside and outside the greenhouse, air conditioning inside the greenhouse, and the appropriate temperature of each bioreactor are maintained throughout the year, so that it is a quantum mechanical physical quantity originating from the sun as a supplement for petroleum, coal, and other stock energy. It is possible to produce a pure organic material with consideration for the environment by photon energy, and a configuration system that can efficiently acquire and store energy.

  Cyanobacteria have the ability to fix air nitrogen, which does not go through a large energy consumption process like the manufacturing method of industrial chemical fertilizer, and this ability peculiar to prokaryotes leads to the production of organic fertilizer. And organic fertilizers containing molecules can be used for plant cultivation without causing environmental pollution.

  It is a longitudinal cross-sectional view of the small-sized transparent (wire netting, tempered glass) and spherical shape aquatic microbiological culture cell according to an embodiment of the present invention. A indicates convection (vertical circulation flow) which is a circumferential flow in the vertical (vertical) direction. B shows a vortex flow (transverse circulation flow) which is a circumferential flow in the horizontal (horizontal) direction.   1 shows the three-stage configuration of the upper, middle and lower cells of the cell and upper and middle connected drains, separation cylinders, mesh cups, overflow and overflow tanks. FIG. This is the angle conversion mechanism and the replenisher part, the gas displacement drilling spoon blade, the coaxial rotating part of the axial flow screw. C indicates the inflow direction of CO ₂ gas and air.   FIG. 2 is a longitudinal sectional view of a cyanobacterial reduction bioreactor system and an oxidation bioreactor system equipped with a greenhouse and a photovoltaic power generation system.   The installation example of the general house of a culture apparatus unit and a building type house is shown.   An interconjugated block diaphragm is shown by connecting a reduced flow culture system and an oxidized fermentation system.

DESCRIPTION OF SYMBOLS 1 Upper culture | cultivation cell 1a Large diameter cylindrical part 1b A spherical body part 1c Small diameter cylindrical projection part 1d The branch pipe 1e for overflows A horizontal rib (transparent glass)
1f Vertical rib (transparent glass)
2 Middle culture cell 3 Lower culture cell 4 Drain valve 5 Connecting rod 6 Cylinder 6a Cylinder 6b Plunger 1
6c Plunger 2
7 Mesh cup (cup with filter)
8 Overflow hose 9 Overflow tank 10 Replenisher tank (head tank)
11 Flow control valve 12 Connecting tube 13 Connecting tube 14 Nozzle (Teflon tube)
15 Geared Motor 16 Drive Shaft 17 Hole Spoon Blade 18 Axial Screw 19 Unit System 20 Bioreactor (Oxidized Type)
21 Greenhouse 22 Solar power generation panel unit 23 Fluorescent lamp 24 Film heater 25 Air conditioning (greenhouse control) system 26 Solenoid (direct acting solenoid)
27 Reflector (mirror)
28 Control box 29 Rack 30 Solenoid (rotary solenoid)

In order to supply the replenisher to the center of the rotary perforated spoon blade 17, the quantitative drip corrosion-resistant resin tube 14, which is notched in a U shape from the center to the top of the spoon blade and inserted from the top, A structure that can rotate without touching. By rotating the rotating direction of the spoon in the direction of travel on the concave side (the rotating direction in FIG. 3), the culture pressure is included to increase the internal pressure on the concave side and the external pressure on the back side (convex side) to be reduced. Through the flow holes processed into the liquid, the liquid flows through at an accelerated flow rate. This through flow creates a turbulent flow behind the spoon, and CO ₂ gas is mixed into the culture solution by the pressurizing effect on the front side (concave surface) of the perforated spoon blade. O ₂ gas is degassed from the culture solution by the pressure reducing effect on the back side (convex surface side) of the spoon from the culture solution, and finally gas is replaced by release into the atmosphere.

Claims (3)

Cultivation of cyanobacteria among microalgae that grow in the hydrosphere in reducing / oxygen-generating microorganisms, this phototrophic organism (autotrophic microorganism) is cultured in a tens of liters of transparent containers (wire-mesh glass) The structure of the tank (cell) is that a large-diameter cylindrical part with an upper gutter with a branch pipe for maintaining the liquid level of the culture medium, a spherical body part, and a small-diameter cylindrical projection on the bottom spherical surface are connected on the center line. Air and CO ₂ gas into the cyanobacterial culture of reduced micro-organisms in the hydrosphere, and a transparent and spherical culture tank (cell) that requires a curved inner surface of the main culture tank structure. while the charged solvent action, pull the O ₂ gas generated by the photosynthesis carbon dioxide assimilation in the culture, at the same time replenishment of new nutrient solution is carried out in a quantitative instillation, the whole in a spherical cell bacteria CO ₂ and inorganic salt evenly rotary gas-liquid mixture having an effect for feeding, the screw type can be maintained for each cell a given flowing form and gas replacement blade, upper coaxially liquid mixture and the gas replacement In order to supply the replenisher to the center of the rotary punching spoon blade, the upper part from the center of the spoon blade is The U-shaped cutout is inserted into the cutout from the top, and the fixed-dose nosepiece is structured so that a flexible chemical-resistant tube can rotate without contact. The culture solution in each cell in the lower stage is cultivated in a culture tank while gently mixing convection and vortex while mixing CO ₂ , air, and supplementary nutrient solution with gas-liquid mixed gas replacement blades and screws by means of shaft rotation. Fluid flow By doing this, the culture tank (cell) which adjusts light absorption and light quantity, and maintains the maximum growth rate under the optimal conditions maintained at the constant temperature (20 ° C. to 40 ° C.) of the input of inorganic salts, gas replacement, and culture medium.   Hydrodynamic theory can be applied to the curvature of the spherical culture tank (cell), and the convection (longitudinal circulation flow) and vortex flow (transverse circulation flow) can be gently configured at regular intervals by the axial flow of the screw. A small-diameter cylindrical tube located in the center of the lower part, which is a fluid culture that performs a photosynthetic reaction mainly during convection. The structure according to claim 1, wherein the cells are aggregated and concentrated in a three-stage structure on the center line of the culture tank (cell), the upper cell, the middle cell, and the lower cell. A movable valve with the cyanobacteria provided at the lower end of the small-diameter cylindrical part is concentrated and concentrated in the lower small-diameter cylindrical part. , The middle than the upper, to flow down by a predetermined amount in the lower than the middle, photosynthesis device apparatus of the culture tank according to claim 1, such as the middle, sequentially increasing the concentration of lower cyanobacteria (cell).   In order to prevent in advance the criticality of the concentration of cyanobacteria divided and proliferated by photosynthesis by the structure described in claims 1 and 2, a switching plunger valve is provided at the lower end of the small-diameter cylindrical tube of the lowermost cell, and the cylinder is Opened, the cyanobacteria gathered and concentrated in this small diameter cylindrical part is sucked into the cylinder and concentrated, secured, and provided with a valve, switched in the take-out direction at a predetermined time cycle, and driven by a plunger The photosynthetic unit device according to claim 1, wherein the photosynthetic unit device is equipped with these, which are pumped by force and received and separated and harvested by a cup in which a mesh is set, and can be installed in a general household in a compact manner.

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