JP3160860B2 - Eradication and antibacterial agents - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a disinfectant / antibacterial agent.

[0002]

2. Description of the Related Art Wood vinegar as a liquid substance obtained by cooling flue gas generated by carbonizing thinned wood (oak) and conifer (pine, cypress) thinned wood is used as an insect repellent, deodorant, activating crops, It is used for many purposes mainly in agriculture such as soil improvement. These carbonized substances are generated from natural substances and have a high decomposability even if they flow out, so they are considered to have little effect on environmental pollution. In these dry distillation liquids, 80 to 90% is moisture, and acetic acid, alcohol, ester, aldehyde, phenol, etc.
Several components are qualitatively known. Therefore,
The soil deodorizing effect is considered to be mainly due to formaldehyde and its derivatives, and also to acetic acid, formic acid, and alcohols. On the other hand, rice husk liquor, which is a dry distillation material of rice husks, has less affinity for tar and other viscous substances than wood vinegar. Is small. Therefore, it has an advantage that it can be easily diluted, and it is presumed that it has more utility value than wood vinegar including economical aspects. In other words, rice husk is generated in large quantities as a by-product of rice agriculture, and new effective use of rice husk can be expected. In addition, various antibacterial products have been developed for bacteria, but most of them use chemical substances, so there are concerns about side effects.

[0003]

An object of the present invention is to provide a useful disinfectant / antibacterial agent which has high antibacterial and antibacterial abilities, has no side effects, and has no smoked odor. .

[0004]

Means for Solving the Problems In order to achieve the above-mentioned object, the present invention comprises a raw rice vinegar component and a diluting component, and the raw rice vinegar component diluted with a physiological saline as the diluting component is used as a whole. Characterized in that there is no smoked odor adjusted to a ratio relationship of 1 to 5% of

[0005]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be described below in detail. The disinfecting and antibacterial agent is composed of raw rice vinegar and a diluting component.
The ratio is adjusted so that the ratio of the raw rice vinegar component diluted with water is 1 to 5% of the whole. <Specific example 1> The ratio of the ratio between the raw rice vinegar component and the diluting component is
Raw rice vinegar is diluted with physiological saline as a diluting component so as to make up 1% of the whole. <Specific Example 2> The ratio of the ratio between the unhulled vinegar component and the diluting component in the disinfecting / antibacterial agent was
Raw rice vinegar is diluted with a physiological saline solution as a diluting component so that the component becomes 3% of the whole. <Specific Example 3> The ratio of the ratio between the unhulled rice vinegar component and the diluting component is as follows.
Raw rice vinegar is diluted with physiological saline as a diluting component so that the vinegar component is 5% of the whole. <Comparative Example 1> The test agent was composed of only physiological saline. <Comparative Example 2> The test agent was configured by diluting with physiological saline as a diluting component so that the ratio relationship between the raw rice vinegar component and the diluting component was 7% of the whole.

[0006] Preparation of the E. coli and Staphylococcus aureus-containing solution to be used for the experiment was carried out by using Eshelichia c.
oli AHU1714) or Staphylococcus
cusaureus cowan) with a platinum loop and sterilized 0.8
The mixture was diluted with 9 ml of 5% saline. Further, this mixed diluted solution was diluted 100 times with physiological saline. In the experiment, first, 8 ml of physiological saline was added to each test tube, and 1 ml of a solution containing coliform bacteria or Staphylococcus aureus was added thereto at a time, and then each of the eradication of the above-mentioned specific examples 1, 2 and 3 was removed.・ Antibacterial agent,
Then, 1 ml of each of the test agents of Comparative Example 2 was added to bring the total volume to 10 ml. In Table 1 below, the result obtained by adding the specific example 1 is referred to as Experimental example 1, the result obtained by adding the specific example 2 is referred to as Experimental example 2, the result obtained by adding the specific example 3 is referred to as Experimental example 3, and the comparative example 2 is added. This was designated as Experimental Example B. In the case of Experimental Example A according to Comparative Example 1, 1 ml each of a solution containing coliform bacteria or Staphylococcus aureus and 9 ml of physiological saline were added to make the total volume of each solution 10 ml. Next, stir with a touch mixer for 30 seconds,
Incubated for hours. Thereafter, 1 ml of the solution in the test tube was taken out and provided for measurement of the viable cell count. For the measurement of Escherichia coli, about 15 ml of desoxycholate medium was added to a petri dish and hardened.
Further, 5 ml was added, and after overlaying, the number of colonies after culturing at 37 ° C for 24 hours was counted. For measurement of Staphylococcus aureus, about 15 ml of a standard agar medium was added to a Petri dish, and after culturing at 37 ° C. for 48 hours, the number of colonies in the Petri dish was counted (see Table 1).
did.

[0007]

[Table 1]

According to Table 1, in Examples 1 to 3, both the coliforms and Staphylococcus aureus were significantly reduced as compared to Example A. As shown in Experimental Example B, the higher the concentration of raw rice vinegar, the greater the effect of this sterilization ability. In Experimental Example 3, about 95% of the coliform bacteria were removed and 100% of the Staphylococcus aureus were removed. On the other hand, in Experimental Example B having a high sterilization ability, there is a smoked smell. From this, it was confirmed that in Experimental Examples 1 to 3 in which the raw rice vinegar concentration was 1 to 5%, the coliform group and Staphylococcus aureus could be efficiently eliminated and there was no smoked smell.

[0009]

[Table 2]

[0010] In Table 2, a solution containing about twice the number of coliform bacteria in the experiment of Table 1 was used, and the same experiment was performed.
It shows the germicidal ability when the temperature was kept for 30 minutes and for 7 days. In the 30-minute incubation, the eradication rates of Experimental Examples 4 and 5 were lower than those of the 2-hour incubation in Table 1, but Experimental Example 6 showed a high 96% eradication rate. Furthermore, when the temperature was kept for 7 days, the number of bacteria was not detected in all of Experimental Examples 4 to 6. Therefore, even when the number of coliforms is large, 9 hours, as in the case of Experimental Example 6, where the raw rice vinegar has a 5% concentration in the 30-minute incubation.
It was confirmed that 5% of the bacteria could be removed, and that it was possible to remove 100% of the unhulled vinegar even in the case of Experimental Example 4 having a concentration of 1%, and that there was no smoked smell when kept warm for 7 days.

[0011] Table 3 shows the measurement results of samples collected from a river (Shinkawa River: No. 1 Shinkawa Bridge, Teine-ku, Sapporo-shi) with a high inflow of treated sewage water and a high quality of coliform bacteria and nutrient salts. is there. Preparation of eradication and antibacterial agents and measurement of coliform bacteria
According to the method in Table 1 described above. That is, 8 ml of the sample was added to each test tube so that the total volume became 10 ml, and the adjusted E. coli group-containing solution and the specific example 1 were added thereto.
Add 1 ml of each of the bactericidal and antibacterial agents of Comparative Example 2 to Comparative Example 2 and stir with a touch mixer for 30 seconds. Then, keep the temperature at 20 ° C for 30 minutes to measure coliform bacteria, and keep the temperature at 20 ° C for 2 hours. Then, the number of general viable bacteria was measured. In Table 3 below, the result obtained by adding the specific example 1 is referred to as Experimental example 7, the result obtained by adding the specific example 2 is referred to as Experimental example 8, the result obtained by adding the specific example 3 is referred to as Experimental example 9, and the comparative example 2 is added. This was designated as Experimental Example E.

[0012]

[Table 3]

[0013]

[Table 4]

Table 3 shows the disinfecting ability of the sample water after incubating for 30 minutes against intestinal bacteria. The disinfecting ability increases as the concentration of rice vinegar increases and the concentration of rice vinegar is 1%. In the case of Example 7, 20%, in the case of Experimental Example 8 in which the concentration of rice vinegar is 3%, 80%, and in the case of Experimental Example 9, in which the concentration of rice vinegar is 5%, 85%.
% Eradication rate. Thus, it was confirmed that in a river with a high degree of contamination, the sterilization ability was increased when the concentration of rice vinegar was 3% or more. Table 4 shows 2 samples for general living bacteria of sample water.
It shows the bactericidal ability after heat retention for 60 hours, and the bactericidal rate is 60% in the case of Experimental Example 7 in which the concentration of rice vinegar is 1%, and the concentration of rice vinegar is 3%.
It is 85% in the case of Experimental Example 8 and 94% in the case of Experimental Example 9 in which the concentration of rice vinegar is 5%. Like the coliform group, the eradication effect increases as the concentration of rice vinegar increases. confirmed. In addition, general viable bacteria are more easily eradicated than the coliform group, which is considered to be due to the effect of the heat retention time. From the above, in the case of Experimental Example 9 in which the concentration of rice vinegar is 5%, it is possible to eliminate 90% or more of the coliform bacteria and / or Staphylococcus aureus by keeping the temperature for about 2 hours, and there is no smoked smell. confirmed.

Table 5 shows the estuary (Otaru Canal:
This is a measurement result of a sample collected from the Nakagawa estuary point on the Temiya side of Otaru City. Preparation of the bacteria-eliminating / antibacterial agent and measurement of the coliform bacteria were performed according to the methods in Table 1 described above. Total volume 1
9 ml of a sample is put into each test tube so as to be 0 ml, and the sterilizing / antibacterial agent of Example 3 and the test agent having a rice vinegar concentration of 10% are added to each tube.
Add 30 ml with a touch mixer and stir for 20 seconds.
Incubated at 2 ° C. for 2 hours. Then, sample 1 ml from the test tube
Was taken out and placed in a sterilized petri dish, and the numbers of general viable bacteria and coliforms were measured. For the measurement of the number of general viable bacteria, about 15 ml of a standard agar medium was added to a Petri dish, and after culturing for 48 hours, the number of colonies in the Petri dish was counted. The measurement of the viable cell count is the same as that in Table 1 described above. In Table 5 below, the one to which the specific example 3 was added was set as an experimental example 10, and the one to which a test agent having a rice vinegar concentration of 10% was added was set as an experimental example F.

[0016]

[Table 5]

Table 5 shows the bactericidal abilities of the coliforms and general viable bacteria after incubation for 2 hours with a sterilizing / antibacterial agent having a rice vinegar concentration of 5%. 80 with live bacteria
%, And the eradication rate was about 10% lower than the experimental examples in Tables 1 and 3 in spite of the small number of viable bacteria in the sample. This means that the sample contains about 80% of seawater, which may have improved the bactericidal resistance of viable bacteria. In this way, by maintaining the temperature for 2 hours using a disinfectant / antibacterial agent having a rice vinegar concentration of 5%, it is possible to remove about 90% or more of both the coliforms and general viable bacteria, and further for 7 days 100% for keeping warm
It was estimated that the bacteria could be removed, and it was confirmed that there was no smoking odor.

[0018]

A. Effects of the Invention According to claim 1, since it has high antibacterial and antibacterial abilities, and has no side effects and no odor of smoking, it is useful for rivers, regulating ponds, sewage treatment plants, etc., and also for cleaning foods and dishes. It can be widely used for applications requiring disinfection, disinfection, and antibacterial.

Claims (1)

(57) [Claims] 1. A smoke comprising a raw rice vinegar component and a diluting component , wherein the ratio of the raw rice vinegar component diluted with physiological saline as a diluting component is adjusted to 1 to 5% of the whole. Disinfectant and antibacterial agent characterized by no odor.