JP3146389U - Microscope with manipulator - Google Patents

Abstract

In a microscope with a manipulator having a revolver capable of switching a plurality of objective lenses, the manipulator can return the manipulator quickly and accurately when the objective lens is switched, and the subsequent processing can be performed smoothly and reliably. An attached microscope is provided.
A guide rail 3 is attached to a microscope body 1 via a support 2 and extends in a direction away from a revolver 17, a manipulator 4 slidably provided on the guide rail 3, and a guide rail 3, when the objective lens 15 is switched, the manipulator 4 is provided so as to be retractable along the guide rail 3 in a direction away from the revolver 3. The contact portion provided on the manipulator 4 and the guide rail 3 are provided. And a stopper that contacts and receives the contact portion when the manipulator 4 slides to the revolver 17 side.
[Selection] Figure 2

Description

The present invention relates to a microscope with a manipulator having a plurality of objective lenses attached to a revolver and having a manipulator for processing an object to be inspected. In particular, the return of the manipulator after retraction when the objective lens is switched. The present invention relates to a device that can prevent the position of the manipulator from being shifted and can perform subsequent processing smoothly and reliably.

Conventionally, when processing a specimen such as a cell or micropart using a manipulator under a microscope, it was attached to a revolver when switching the objective lens to change the magnification of the microscope. Since the objective lens group and the micro tool attached to the manipulator physically interfere with each other, once the micro tool is removed or the manipulator is moved to another location, the objective lens is switched and then returned to the original state. The subsequent processing was performed.

However, in the microscope with a manipulator as described above, once the micro tool is removed or the manipulator is moved to another place, these are accurately returned to the original positions after the objective lens is switched. In particular, in the fine work of the micron order, the accuracy of this return is lacking, which hinders the subsequent processing of the specimen.

In addition, if the manipulator is moved and retracted by manipulator operation, the stroke for movement must be provided longer than the practical movable range, and it takes time and effort to manufacture. There is a problem that it takes a long time to evacuate and return.

The present invention has been made to solve the above-described problems.

The invention according to claim 1 includes a revolver and a plurality of switchable objective lenses attached to the revolver, a microscope main body for magnifying a test object, and the microscope main body attached to the microscope main body via a support. , A guide rail extending in a direction away from the revolver, a manipulator provided slidably on the guide rail, and a micro tool that is detachably attached to the manipulator and performs processing on the test object And when the objective lens is switched, the guide rail is provided so that the manipulator can be retracted along the guide rail in a direction away from the revolver, and a contact portion provided in the manipulator; and Provided on the guide rail, when the manipulator slides to the revolver side, it contacts the contact part A microscope with manipulator and a stopper for receiving the manipulator Te.

The invention according to claim 2 is the microscope with a manipulator according to claim 1, wherein a pin body attached to the manipulator is movable up and down, and a blocking member attached to the guide rail and capable of locking the pin body. The blocking member is configured to lock the pin body when the abutment portion of the manipulator is in contact with the stopper and the pin body is moved downward, so that the manipulator When the pin body is retracted upward, the pin body is unlocked and the manipulator slides to the side opposite to the revolver of the guide rail. Is allowed.

The invention according to claim 3 is the microscope with manipulator according to claim 2, further comprising a biasing means for constantly biasing the pin body downward, wherein the lower end portion of the pin body faces downward. The diameter of the pin member is gradually reduced, and when the pin body is urged downward, the block member has a line contact or surface contact with the block member at a portion opposite to the revolver at the lower end of the pin body. An inclined portion is provided.

According to a fourth aspect of the present invention, in the microscope with a manipulator according to the first to third aspects, the guide rail is inclined downward toward the revolver side.

According to the microscope with a manipulator according to claim 1, a guide rail attached to the microscope body via a support and extending in a direction away from the revolver, and a manipulator provided slidably on the guide rail; The guide rail is provided so that the manipulator can be retracted in a direction away from the revolver along the guide rail when the objective lens is switched.
When switching the objective lens, the manipulator can be easily and quickly retracted along the guide rail, and the objective lens can be prevented from interfering with the manipulator and / or the micro tool.
Moreover, since it has a contact portion provided on the manipulator, and a stopper provided on the guide rail, and when the manipulator slides to the revolver side, it contacts the contact portion and receives the manipulator.
When the manipulator once retracted is returned to the original position, the manipulator can be returned to the original position with high reproducibility, and subsequent processing by the micro tool can be performed smoothly and reliably.

According to the invention described in claim 2, in addition to the effect of the microscope with a manipulator according to claim 1, the pin body attached to the manipulator and movable up and down, and attached to the guide rail, the pin body is locked. A blocking member that is capable of locking the pin body when the abutment portion of the manipulator is in contact with the stopper and the pin body is moving downward. Is prevented from reversing to the side opposite to the revolver of the guide rail, and when the pin body is retracted upward, the pin body is unlocked and the manipulator is opposite to the revolver of the guide rail. To allow sliding to the side,
Once the abutment portion abuts against the stopper and receives the manipulator, the pin body 41 is in the state where it is locked to the blocking member 34. It is possible to prevent the guide rail from going backward, and to return the manipulator to the original position with higher reproducibility.

According to the invention described in claim 3, in addition to the effect of the microscope with a manipulator described in claim 2, the pin body is provided with a biasing means that normally biases the pin body downward, and the pin body has a lower end of the pin body. When the pin body is gradually reduced in diameter toward the lower side and the pin body is biased downward, the blocking member is in line contact or surface contact with the blocking member at a portion opposite to the revolver at the lower end portion of the pin body. In addition, since the blocking member is provided with an inclined portion,
The manipulator is pressed toward the revolver due to the urging force of the pin body and the inclination of the inclined portion, and in combination with the contact between the contact portion and the stopper, the positioning accuracy of the manipulator can be improved. The manipulator can be more stably fixed by line contact or surface contact with the blocking member.

According to the invention described in claim 4, in addition to the effect of the microscope with a manipulator according to claims 1-3, the guide rail is inclined downward toward the revolver side,
The stable position of the manipulator is on the revolver side in the guide rail, and the contact state between the contact portion and the stopper can be maintained more reliably, and the positioning accuracy of the manipulator can be improved.

An embodiment of the present invention will be described with reference to FIGS. In FIG. 1, A shows a microscope with a manipulator according to the present invention, and the microscope A with a manipulator is roughly composed of a microscope body 1, a guide rail 3, a manipulator 4, and a micro tool 5. .

As shown in FIGS. 1 and 2, the microscope main body 1 includes a revolver 17 (a rotating device capable of switching a plurality of objective lenses having different magnifications) and a plurality of switchable objective lenses 15 attached to the revolver 17 ( 15a, 15b,...) And a specimen b (for example, a cell for performing artificial insemination, a micro article such as a micro machine), and a mirror base 13 serving as a pedestal And on the back side of the mirror leg 13, the mirror column 12 serving as a column of the microscope main body 1 is positioned substantially above the mirror column 12, and the mirror 2 is interposed via the support 2. An upper member 11 attached to the upper end of the column 12 and a sample stage 16 on which a petri dish c or the like containing the test object b is placed.

Here, the upper member 11 includes an eyepiece lens 14, a revolver 17, and an objective lens 15 (15 a, 15 b,...), And the upper member 11 illuminates the test object b. The light source lamp 18 is also provided, and the optical path is optically refracted by a half mirror (not shown) provided in the upper member 11 to irradiate the object b with illumination light.

Further, the sample stage 16 is configured such that the petri dish c or the like placed on the sample stage 16 is viewed from the front of the microscope body 1 in the left-right direction (X-axis direction), the front-rear direction (Y-axis direction), and the up-down direction (Z-axis direction). 1-4, for example, as shown in FIGS. 1 to 4, it can be moved horizontally by the X-axis knob 16a and the Y-axis knob 16b and vertically by the Z-axis knob 16c. The object b to be magnified can be moved to a desired position, or the focal position can be changed. In the present embodiment, the microscope A with a manipulator configured to manually move the sample stage 16 by providing the knobs 16a, 16b, and 16c is illustrated, but the sample stage 16 may be moved by an actuator. In such a case, actuators (not shown) may be provided corresponding to the X-axis direction, the Y-axis direction, and the Z-axis direction, respectively, and the sample stage 16 may be moved by remotely operating the actuator. Further, the knobs 16a, 16b, and 16c described above and the actuator may be used together by switching.

The guide rail 3 described above is a member that is attached to the microscope body 1 via the support 2 and extends away from the revolver 17 as shown in FIGS. 2, 5, 6 (a) and the like. Yes, when the objective lens 15 attached to the revolver 17 is switched, the manipulator 4 can be retracted along the guide rail 3 in the direction away from the revolver 17 (the direction of the arrow d1 in FIGS. 6A and 6B). For example, as shown in FIG. 7, it is integrally fixed to the support 2 with bolts or the like via attachment members 21 and 22.

In the present embodiment, the support 2 is fixed so as to be sandwiched between the upper end of the lens column 12 and the upper member 11 as shown in FIG. Instead of being sandwiched between the upper end portion of the lens column 12 and the upper member 11, the upper end portion of the lens column 12 may be contacted and fixed. The support body 2 is formed of a metal material such as aluminum or titanium having high rigidity so that the manipulator 4 having a large mass can be reliably supported.

The manipulator 4 described above is slidably provided on the guide rail 3 as shown by the phantom line in FIG. 7. Specifically, the mounting member 46, the sliding member 47, and the bearings 31, 31 are provided. It is attached to the guide rail 3 via. Further, as shown in FIG. 6A, this manipulator 4 is for attaching a micro tool 5 via an arm 44, and any known manipulator 4 can realize a precise movement in the XYZ axis directions. For example, as shown in FIGS. 6A and 6B, knobs 4a, 4b, and 4c are provided, and these are rotated and operated manually, or an actuator (not shown) is provided. May be operated automatically or by providing both knobs 4a, 4b, and 4c and actuators and switching them as appropriate (in this embodiment, the knobs 4a and 4b are attached to the guide rail 3 (support 2) side). It may be attached to the manipulator 4 side).

As shown in FIGS. 6A and 6B, the manipulator 4 is provided with a contact portion 46 a via an attachment member 46, and the manipulator 4 is disposed on the guide rail 3 on the revolver 17 side. When sliding to the left (the left side of FIG. 6 (b)), a stopper 33 is provided to contact the contact portion 46a and receive the manipulator 4, and by contact between the contact portion 46a and the stopper 33, Positioning on the revolver 17 side in the sliding direction of the guide rail 3 of the manipulator 4 is performed.

The above-described microtool 5 is detachably attached to the manipulator 4 and directly acts on the test object b to perform various processes on the test object b. Collecting micro-pipettes that can insert nuclei into the electrodes, electrode probes that cause electrophysiological reactions while directly applying voltage to the specimen b, and collecting foreign matter on the silicon wafer A needle or a knife that cuts the specimen b is used.

By the way, as shown in FIG. 6A, FIG. 8, etc., a pin body 41 that can be moved up and down is attached to the manipulator 4, and a blocking member 34 that can lock the pin body 41 is attached to the guide rail 3, respectively. May be. In the state where the contact portion 46 a is in contact with the stopper 33, the blocking member 34 is engaged with the pin body 41 when the pin body 41 has advanced downward, so that the manipulator 4 can move the revolver 17 of the guide rail 3. And when the pin body 41 is retracted upward, the pin body 41 is unlocked so that the manipulator 4 can move the guide rail 3 to the opposite side (the right side of FIG. 6A). When the pin body 41 is locked to the blocking member 34, the manipulator 4 is moved to the side opposite to the revolver 17 due to reaction or vibration. It is possible to prevent the guide rail 3 from going backward, and the manipulator 4 can be positioned with higher accuracy.

Further, when the pin body 41 is provided, as shown in FIG. 8, a biasing means 49 such as a spring that normally biases the pin body 41 downward is provided, and the lower end portion of the pin body 41 is provided. As shown in FIG. 6A, when the pin body 41 is urged downward as shown in FIG. 6 (a), the portion of the lower end portion 41a of the pin body 41 opposite to the revolver 17 is gradually reduced in diameter. In this case, the blocking member 34 may be provided with an inclined portion 34 a so as to be in line contact or surface contact with the blocking member 34.

By forming the pin body 41 and the blocking member 34 in this way, the manipulator 4 is pressed toward the revolver 17 by the urging force of the pin body 41 and the inclination of the inclined portion 34a, and the contact portion 46a and the stopper 33 are The positioning accuracy of the manipulator 4 can be improved in combination with the contact, and the manipulator 4 can be more stably fixed by the line contact or the surface contact between the pin body 41 and the blocking member 34. .

As described above, the microscope A with a manipulator of the embodiment configured as described above is used as described below. That is, the manipulator 4 is operated under the microscope using the arbitrary objective lens 15 (for example, the objective lens 15a) attached to the revolver 17 as shown in FIG. When the objective lens 15 (15a) is switched to another objective lens 15 (for example, the objective lens 15b) in order to change the enlargement magnification after performing various processes, first, FIGS. 10) From the state shown in FIG. 10A, the knob 42 is operated to retract the pin body 41 upward against the urging force of the urging means 49. At this time, as shown in FIG. 10B, the blocking member 34 is separated from the pin body 41 and the locking of the pin body 41 to the blocking member 34 is released.

Then, when the bent piece 43 is picked with a finger and the manipulator 4 is pulled in the direction of the arrow d1, as shown in FIGS. 9A and 9B, the contact portion 46a and the stopper 33 are separated from each other and the manipulator 4 slides along the guide rail 3, and the micro tool 5 can be moved in a direction away from the revolver 17 (a direction away from the revolver 17).

In this state, the revolver 17 is rotated to switch the objective lens 15 to a desired one (objective lens 15b), and then the manipulator 4 is moved in the direction indicated by the arrow d2 in FIG. When it slides (returns), it returns to the state of FIGS. 6A, 6B, and 10A again. At this time, the lock of the pin body 41 is released as the manipulator 4 slides. Then, as shown in FIG. 10 (d), the pin body 41 is biased downward, and when the contact portion 46a comes into contact with the stopper 33, the lower end of the pin body 41 is reached. The portion 41a is again locked to the inclined portion 34a (see FIG. 10A), and the manipulator 4 is returned to the original position and fixed.

By the way, it is preferable that the guide rail 3 mentioned above inclines downward toward the revolver 17 side, as shown to FIG. 2, FIG. 6 (a). By this inclination, the stable position of the manipulator 4 becomes the revolver 17 side in the guide rail 3, and the contact state between the contact portion 46a and the stopper 33 can be maintained more reliably.

In the case of providing the inclination as described above, as shown in FIG. 6B, the manipulator 4 is provided with the hook 48 via the mounting member 46, and the guide rail 3 is provided with the locking member 35. As shown in FIG. 9B, the hook 48 may be locked to the locking member 35 when the lever is retracted to the side opposite to the revolver 17 (right side of FIG. 6B). By locking the hook 48, it is possible to prevent the manipulator 4 from inadvertently sliding toward the revolver 17 when the objective lens 15 is switched.

In the above-described embodiment, the example in which the guide rail 3 is provided on the right side when viewed from the front of the microscope main body 1 has been mainly described. However, as shown by the phantom lines in FIGS. In particular, when provided on both the left and right sides, the weight balance between the left and right sides of the microscope body can be further maintained, and the manipulator 4 can be stably evacuated and retracted when the objective lens 15 is switched. it can.

It is the schematic perspective view which showed one Example of the microscope with a manipulator which concerns on this invention. FIG. 2 is a schematic front view of FIG. 1. FIG. 2 is a schematic plan view of FIG. 1. FIG. 2 is a schematic diagram on the right side of FIG. 1. It is the schematic perspective view which decomposed | disassembled and showed a part of FIG. It is the schematic which expanded and showed the site | part of the manipulator of FIG. 1, Fig.6 (a) has shown the front view, FIG.6 (b) has each shown the top view. It is the cross-sectional schematic cut | disconnected by the XX line of Fig.6 (a). It is the cross-sectional schematic cut | disconnected by the YY line | wire of Fig.6 (a). FIGS. 9A and 9B are schematic views illustrating the operating state of FIG. 6, and FIG. 9A corresponds to FIG. 6A and FIG. 9B corresponds to FIG. It is the schematic which showed the operation state of FIG. 6, Comprising: It is a figure for demonstrating the positional relationship of a pin body and a prevention member, FIG.10 (a) is a figure by which the pin body is latched by the prevention member. 10 (b) is a view when the locking of the pin body to the blocking member is released, FIG. 10 (c) is a view when the manipulator is retracted, and FIG. 10 (d) is a view during operation. Respectively.

Explanation of symbols

A Microscope with manipulator b
DESCRIPTION OF SYMBOLS 1 Microscope main body 15, 15a, 15b, 15c Objective lens 17 Revolver 2 Support body 3 Guide rail 33 Stopper
4 Manipulator 46a Contact part 5 Micro tool

Claims (4)

A microscope main body having a revolver and a plurality of switchable objective lenses attached to the revolver, and magnifying a test object,
A guide rail attached to the microscope body via a support, and extending in a direction away from the revolver;
A manipulator slidably provided on the guide rail;
A micro tool that is detachably attached to the manipulator and performs processing on the test object;
The guide rail is provided so that the manipulator can be retracted in a direction away from the revolver along the guide rail when the objective lens is switched.
A contact portion provided on the manipulator;
A microscope with a manipulator, provided on the guide rail, comprising a stopper that receives the manipulator by contacting the contact portion when the manipulator slides toward the revolver. A pin body that can be moved up and down attached to the manipulator, and a blocking member that is attached to the guide rail and can lock the pin body;
In the state where the abutment portion of the manipulator is in contact with the stopper, the blocking member locks the pin body when the pin body advances downward, and the manipulator is opposite to the revolver of the guide rail. And preventing the pin body from moving backward, allowing the manipulator to slide to the side opposite to the revolver of the guide rail when the pin body is retracted upward. The microscope with a manipulator according to claim 1. It is provided with a biasing means that normally biases the pin body downward,
The pin body, the lower end portion of the pin body is gradually reduced in diameter downward,
When the pin body is biased downward, the blocking member is provided with an inclined portion so as to be in line contact or surface contact with the blocking member at a portion of the pin body opposite to the revolver. The microscope with a manipulator according to claim 2.   The microscope with a manipulator according to any one of claims 1 to 3, wherein the guide rail is inclined downward toward the revolver side.

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