JP3132187U - Housing for microchannel array - Google Patents

Abstract

Disclosed is a microchannel array housing capable of reducing leakage of a liquid measurement sample.
A microchannel array housing according to the present invention is a microchannel array housing in which a resin microchannel array 3 having a flow path forming region 38 is incorporated, and includes a first jig 1, a transparent plate 5, The second jig 2 having the buffer rubber 4 and the rod-shaped structure 21 is provided. And the contact surface with the resin-made microchannel array 3 in the transparent plate 5, the contact surface with the resin-made microchannel array 3 in the buffer rubber 4, the contact surface with the rod-shaped structure 21 in the buffer rubber 4, and the rod In the contact surface of the shaped structure 21 with the buffer rubber 4, the common common region 50 includes at least the flow path forming region 38 and covers a part of the resin microchannel array 3.
[Selection] Figure 2

Description

  The present invention relates to a housing for a microchannel array.

  The fluidity of the fluid containing the formed component becomes more important as the diameter of the tube or the channel approaches the formed component diameter because the formed component may often cause a channel failure. A typical example is blood fluidity. In capillaries that play a role in blood circulation, in many cases there is an inverted relationship that the blood vessel diameter is smaller than the red blood cell diameter or the white blood cell diameter, and the blood fluidity, more precisely, the blood cell fluidity It is an extremely important factor that affects blood flow.

  Recent studies have shown that blood fluidity changes with environmental factors such as diet, exercise, stress, and temperature. For this reason, the quality of capillary blood flow resulting from changes in blood fluidity directly affects the metabolism and activity of tissues, and it can be said that the relationship between health and disease is extremely large. Accordingly, there is a need for simple and reproducible measurement of blood fluidity for purposes such as health assessment, disease prevention, and early diagnosis of disease.

Therefore, a silicon microchannel array manufactured by a semiconductor microfabrication technique for microfabricating a flow path on a silicon substrate has been proposed. In this technique, patterning is performed on a silicon substrate (first substrate) by a photolithographic method, grooves are formed on the silicon substrate by wet or dry etching methods, and the second substrate is a flat plate on the surface on which the grooves are formed. This is a method of forming a blood flow path by joining the two. With this technology, it is possible to design the width and depth ratio, spacing, etc. of the minute flow path according to the purpose, and it is possible to directly observe the actual flow in the flow path through the transparent plate. . In the case of such a silicon microchannel array, 1) the material cost of the silicon substrate is high, 2) the processing cost is high because photolithography is performed for each one, and 3) the fineness for each one is small. Recently, resin microchannel arrays made of resin have been developed because there are practical problems such as inconsistencies in the dimensional accuracy of the flow path and 5) incineration not possible (Patent Literature). 1). The resin-made microchannel array is obtained by molding a first substrate by injection molding using a mold in which convex portions corresponding to the flow paths are formed, and joining a flat second substrate to the first substrate.
JP 2005-265634 A

  However, such a resin microchannel array has a drawback that warpage occurs due to the storage state due to cooling and moisture absorption after molding, and a liquid measurement sample leaks from a gap formed between the first substrate and the second substrate. Was. For this reason, a means for preventing leakage of the resin microchannel array has been desired.

  The present invention has been made in view of the above problems, and an object of the present invention is to provide a microchannel array housing that can reduce leakage of a liquid measurement sample.

  A microchannel array housing according to the present invention is a microchannel array housing into which a resin microchannel array having a flow path forming region is incorporated, and is formed larger than the first jig having an observation hole and the observation hole. A transparent plate disposed on the opposite side of the observation hole, a buffer rubber disposed on the opposite side of the transparent plate with the resin microchannel array interposed therebetween, A first rubber-like structure that is disposed on the opposite side of the buffering rubber to the resin microchannel array and that presses the transparent plate, the resin microchannel array, and the buffering rubber toward the first jig side. Two jigs, a contact surface of the transparent plate with the resin microchannel array, and the resin microchannel in the buffer rubber. The contact surface with the array, the contact surface of the buffer rubber with the rod-shaped structure, and the contact surface of the rod-shaped structure with the buffer rubber are formed with the transparent plate in the resin microchannel array. A common area that is common when moved in the vertical direction with respect to the contact surface includes at least the flow path forming area and covers a part of the resin microchannel array. Thereby, the leakage of the liquid measurement sample can be reduced.

  Further, in the above microchannel array housing, the part of the resin microchannel array covered by the common region may be a region that coincides with the flow path forming region, or a region in the vicinity thereof. .

  In the above microchannel array housing, the contact surface of the cushioning rubber with the resin microchannel array coincides with the flow path forming area or is in the same area as the vicinity of the flow path forming area. You may do it.

  Further, in the above microchannel array housing, the flow path forming area of the resin microchannel array is substantially rectangular, and the common area is made of the resin in the long side direction of the flow path forming area. The entire area of the microchannel array may be covered.

In the microchannel array housing, the flow path forming region of the resin microchannel array has a substantially rectangular shape, and the common region is the flow path in the short side direction of the flow path forming region. Only the formation region may be covered.
By adopting these configurations, the flow path forming region is effectively pressed, and at least the entire periphery of the end of the flow path forming region has an adhesive property between the first substrate and the transparent plate constituting the resin microchannel array. improves.

  ADVANTAGE OF THE INVENTION According to this invention, the housing for microchannel arrays which can reduce the leakage of the liquid measurement sample can be provided.

  The configuration of the microchannel array device will be described with reference to FIGS. FIG. 1 is an exploded perspective view showing the configuration of the microchannel array device. FIG. 2 is a schematic diagram showing the configuration of the microchannel array device. FIG. 2A is a top view showing the configuration of the microchannel array device. FIG.2 (b) is AA sectional drawing of Fig.2 (a). FIG.2 (c) is BB sectional drawing of Fig.2 (a). 3A is a top view of the cushioning rubber 4, and FIG. 3B is a cross-sectional view taken along line AA of FIG. 3A.

  As shown in FIG. 1, the microchannel array device includes a resin-made microchannel array 3 in a microchannel array housing including a first jig 1, a transparent plate 5, a buffer rubber 4, and a second jig 2. It has the structure which incorporated. The 1st jig | tool 1 has a cylindrical recessed part, and has a circular observation hole in the center part of the upper surface. As shown in FIG. 2 (b), the first jig 1 is formed with wings 61 over the entire circumference of the end. The outer periphery of the wing 61 has a rectangular shape. The transparent plate 5 has a disk shape larger than the observation hole of the first jig 1 and is disposed on the opposite side of the observation hole. Thereby, the transparent plate 5 is supported from the 1st jig | tool 1 side. In the case of the transparent plate 5 shown in FIG. 2, a rectangular convex portion is formed at the center of the disc-shaped base. Then, the transparent plate 5 is inserted from the opening of the concave portion of the first jig 1 so that the surface opposite to the convex portion forming surface is in contact with the first jig 1, and the observation surface of the resin microchannel array 3 to be described later Use as The contact surface of the transparent plate 5 with the resin microchannel array 3 has a size that can cover all the flow paths of the resin microchannel array 3. In the case of a shape in which a convex portion is provided on a disk-shaped base like the transparent plate 5 shown in FIG. 2, the top surface of the convex portion is in contact with the resin microchannel array 3, The surface becomes the contact surface.

  The transparent plate 5 has a thickness of 0.5 to 2 mm and has a light transmittance of 80% or more without any problem, soda glass, inorganic substances such as quartz, acrylic resin, cycloolefin resin, styrene resin, acrylic Thermoplastic resins such as styrene-based copolymers and polycarbonate-based resins, thermosetting resins, and photocurable resins can be used. The flow path of the resin microchannel array 3 can be directly observed through such a transparent plate 5, and appropriate measures such as adjustment and stop of the flow path can be taken. Here, the transparent plate 5 side of the first jig 1 is the anti-viewing side, and the side opposite to the transparent plate 5 of the first jig 1 is the viewing side (observation side).

  The resin microchannel array 3 is formed with a flow path such as a large flow path or a fine flow path, and is disposed on the opposite side of the transparent plate 5 from the first jig 1. The large flow path is provided in the vicinity of two opposing sides of the resin microchannel array 3. As shown in FIG. 1, one large flow path (first flow path) has a sample introduction hole 31 and the other large flow path (second flow path) has a sample discharge hole 32. And it has a fine channel between these large channels. That is, the large flow path is disposed to face the fine flow path. The flow channel formation region 38 is a region where a flow channel other than the sample introduction hole 31 and the sample discharge hole 32 is formed in the resin microchannel array 3. The details of the resin microchannel array 3 will be described later.

  The buffer rubber 4 is disposed on the opposite side of the transparent plate 5 of the resin microchannel array 3. That is, it is arranged on the opposite side of the transparent plate 5 from the first jig 1 with the resin microchannel array 3 interposed therebetween. The buffer rubber 4 is disposed between a rod-shaped structure 21 of the second jig 2 described later and the resin microchannel array 3. Thus, since the resin-made microchannel array 3 is pressed by the rod-shaped structure 21 with the buffer rubber 4 interposed, the resin-made microchannel array 3 is uniformly loaded. The buffer rubber 4 is rectangular and has a size that can cover all the flow paths of the resin microchannel array 3, that is, the flow path forming region 38. The buffer rubber 4 has a thickness of 0.5 to 3 mm, and for example, natural rubber, synthetic rubber such as NBR, EPDM, CR, or thermoplastic elastomer such as styrene, olefin, or urethane can be used. As shown in FIG. 3, the buffer rubber 4 has a sample introduction path 41 and a sample discharge path 42. Moreover, the diameter of the sample introduction path 41 and the sample discharge path 42 is preferably 0.5 to 3 mm, and is adjusted to the diameter and arrangement of the sample introduction hole 31 and the sample discharge hole 32 of the resin microchannel array 3. Thus, as shown in FIG. 1, the transparent plate 5, the resin microchannel array 3, and the buffer rubber 4 are sequentially arranged from the first jig 1 side in the recess of the first jig 1. ing.

  The second jig 2 is disposed on the opposite side of the buffer rubber 4 from the resin microchannel array 3. The 2nd jig | tool 2 has the rod-shaped structure 21 in the jig | tool center part. Further, as shown in FIG. 2B, the second jig 2 is formed with wings 62 over the entire periphery of the end. The outer periphery of the wing portion 62 has a rectangular shape. Then, the wing portion 61 of the first jig 1 and the wing portion 62 of the second jig 2 are bonded or welded, and the first jig 1, the transparent plate 5, the resin microchannel array 3, the buffer rubber 4, And the 2nd jig | tool 2 is comprised integrally. Further, the rod-shaped structure 21 of the second jig 2 is formed so as to protrude from the bottom surface of the second jig 2 on which the wing portion 62 is formed to the shock absorbing rubber 4 side. With this rod-shaped structure 21, the buffer rubber 4, the resin microchannel array 3, and the transparent plate 5 are pressed from the second jig 2 side and pressed against the first jig 1 to fix them. The rod-shaped structure 21 of the second jig 2 has a sample introduction tube 22 and a sample discharge tube 23. The sample introduction tube 22 and the sample discharge tube 23 preferably have a diameter of 0.5 to 3 mm, and are adapted to the diameter and arrangement of the sample introduction hole 31 and the sample discharge hole 32 of the resin microchannel array 3. That is, in a state where these are combined, the sample introduction hole 31, the sample introduction path 41, and the sample introduction tube 22 become a series of tubular sample inlets. In addition, the sample discharge hole 32, the sample discharge path 42, and the sample discharge pipe 23 are also a series of tubular sample discharge ports. The tip of the rod-shaped structure 21 has a smooth surface other than the sample introduction tube 22 and the sample discharge tube 23, and the cross-sectional shape of the rod-shaped structure 21 is the flow path formation on the resin microchannel array 3. It has a cross-sectional shape that can cover the region 38.

  Since transparency other than the observation surface is not required, the wing 61 of the first jig 1 and the wing 62 of the second jig 2 can be bonded or welded as the first jig 1 and the second jig 2. In addition, any material having strength that does not break during pressure bonding (for example, an inorganic material such as metal, glass, or ceramic, or a resin) can be used. Then, the wings 61 and 62 of the jigs 1 and 2 of the microchannel array housing incorporating the resin microchannel array 3 are bonded or welded together. A weight of 1 to 20 kg, preferably 5 to 10 kg is applied while pressing the second jig 2 against the first jig 1 at the time of bonding or welding. In addition, in order to complete the bonding while applying a load during bonding or welding, methods such as an acrylate-based instantaneous adhesive, hot plate welding, vibration welding, ultrasonic welding, laser welding, and the like can be used. The microchannel array device is configured as described above.

  In the case of the microchannel array device described above, the contact surface of the transparent plate 5 with the resin microchannel array 3, the contact surface of the buffer rubber 4 with the resin microchannel array 3, and the rod-like structure 21 of the buffer rubber 4. And the contact surface with the cushioning rubber 4 in the rod-shaped structure 21 are common when the contact surface with the transparent plate 5 in the resin microchannel array 3 is moved in the vertical direction. The common region 50 includes at least the flow path forming region 38 and is configured to cover a part of the resin microchannel array 3. That is, in the top view shown in FIG. 2A, the common region 50 includes at least the flow path forming region 38 and is configured to cover a part of the resin microchannel array 3. With such a configuration, the flow path forming region 38 of the resin microchannel array 3 can be covered with the minimum area of the contact surface. Here, the part of the resin-made microchannel array 3 covered with the common region 50 is preferably a region coinciding with the flow path forming region 38 or a region in the vicinity thereof.

  In the case of FIG. 2, the contact surface of the transparent plate 5 with the resin microchannel array 3, the contact surface of the buffer rubber 4 with the resin microchannel array 3, and the contact surface of the buffer rubber 4 with the rod-shaped structure 21. The contact surface of the rod-shaped structure 21 with the buffer rubber 4 is substantially the same except for the sample introduction hole 31 and the sample discharge hole 32 in the top view shown in FIG. That is, all the contact surfaces described above substantially coincide with the common region 50 except for the sample introduction hole 31 and the sample discharge hole 32. The common area 50 substantially coincides with the flow path forming area 38, but may be slightly larger than the flow path forming area 38, for example. Specifically, the flow path forming region 38 has a rectangular shape, and the common region 50 may cover the entire region of the resin-made microchannel array 3 in the long side direction of the flow path forming region 38. The common area 50 may cover only the flow path forming area 38 in the short side direction of the flow path forming area. In this case, in the cross-sectional view shown in FIG. 2B, the outer shape of the entire resin microchannel array 3 and the common region 50 substantially coincide. In the cross-sectional view shown in FIG. 2C, the common region 50 substantially coincides with the outer shape of the flow path forming region 38 of the resin microchannel array 3.

  In other words, it is only necessary that a portion overlapping each other in the top view among the contact portions with the members that contact both surfaces of the resin microchannel array 3 can cover the flow path forming region 38. In addition, when a member having low rigidity such as the buffer rubber 4 is in contact with the resin-made microchannel array 3, the size of the overlapping portion is larger than the flow path forming region 38, for example, a resin-made microchannel. It may be the size of the entire array 3. At this time, if the surface of the buffer rubber 4 opposite to the resin-made microchannel array 3 and the contact portion between the highly rigid member such as the second jig 2 substantially coincide with the flow path forming region 38. Good. That is, of these all contact parts, the overlapping part in the top view may be substantially coincident with the flow path forming region 38.

  By incorporating the resin microchannel array 3 into the microchannel array housing having the above-described configuration, at least a part of the resin microchannel array 3 is subjected to weighting. When the jig is pressed with the same force, the pressure increases as the area where the resin microchannel array 3 is subjected to weighting, that is, the area of the common region 50 is smaller. Therefore, as described above, by setting the common region 50 smaller than the outer shape of the resin microchannel array 3 as a whole, the flow path forming region 38 of the resin microchannel array 3 is effectively weighted. . Since the microchannel array in the present invention is made of resin, warping is likely to occur. The warping here is warped so as to be convex toward the first jig 1 side of the resin microchannel array 3. If such warping occurs, the liquid measurement sample that has flowed into the resin microchannel array 3 leaks, which is not preferable. Therefore, as described above, the flow path forming region 38 of the resin microchannel array 3 into which the liquid has flowed is effectively pressed to correct the warpage of the portion. Thereby, the leakage of the measurement sample of the liquid that has flowed into the resin-made microchannel array 3 can be reduced, and the liquid sample can be accurately measured.

  Next, the resin-made microchannel array 3 will be described in detail with reference to FIG. FIG. 4 is a schematic diagram showing the configuration of the resin microchannel array 3. FIG. 4A is a top view showing the configuration of the first substrate 36 on which the flow paths of the resin microchannel array 3 are formed. FIG. 4B is a cross-sectional view taken along line AA of the first substrate 36 of FIG. 4A and the transparent plate 5 as a second substrate that is in close contact with or bonded to the first substrate 36. 4C is a BB cross-sectional view of the first substrate 36 of FIG. 4A and the transparent plate 5 that is in close contact with or bonded to the first substrate 36.

  The first substrate 36 of the resin microchannel array 3 and the transparent plate 5 having a flat surface on the contact surface with the first substrate 36 are in close contact with each other or joined together. On the first substrate 36, a groove and a recessed region as shown in FIG. 4 are provided. Specifically, a first large flow path 33a, a second large flow path 33b, a first fine flow path 34a, a second fine flow path 34b, and a wall portion 35 are provided. A portion on the first substrate 36 where these flow paths are provided is defined as a flow path forming region 38. The outer periphery of the flow path forming region 38 has a substantially rectangular shape. The first substrate 36 is formed larger than the flow path forming region 38, and the flow path forming region 38 is formed at a substantially central portion of the first substrate 36. That is, the first substrate 36 is provided with a flow path forming area 38 and a frame area 39 provided so as to surround the flow path forming area 38.

  The first large flow path 33a and the second large flow path 33b have rectangular depressions (concave portions). The first large flow path 33a is formed in the vicinity of one side of the first substrate 36, and the second large flow path 33b is formed in the vicinity of one side opposite to the one side on which the first large flow path 33a is formed. From the first large flow path 33a and the second large flow path 33b, a plurality of long fine groove groups, a first fine flow path 34a and a second fine flow path 34b, are provided toward the center. In FIG. 4, there are three first microchannels 34a and two second microchannels 34b, which are formed alternately and parallel to each other. Of course, the number of the fine flow paths 34a and 34b is not limited to three and can be any number. And the wall part 35 will be formed between adjacent groove | channels (microchannel 34a, 34b). The wall portion 35 does not divide the completely adjacent fine flow paths 34a and 34b, and is provided with a large number of minute grooves. A minute groove that communicates with the minute flow paths 34a and 34b is a flow path.

  The first substrate 36 is provided with a sample introduction hole 31 into which a physiological saline as a measurement sample, a blood sample, and a reagent are introduced. The sample introduction hole 31 is a through hole provided in the first large flow path 33 a of the first substrate 36. Further, the first substrate 36 is provided with a sample discharge hole 32 for discharging the physiological saline, blood sample, and reagent that have flowed from the sample introduction hole 31. The specimen discharge hole 32 is a through hole provided in the second large flow path 33 b of the first substrate 36.

  As shown in FIGS. 4B and 4C, the transparent plate 5 having a flat surface is superimposed on the surface of the first substrate 36 on which the flow path is formed, and is adhered or bonded. Thereby, the groove of the flow path forming region 38 forms an internal space between the first substrate 36 and the transparent plate 5. These internal spaces have a structure communicating from the sample introduction hole 31 to the sample discharge hole 32.

  When a blood sample or the like flows into the sample introduction hole 31, it flows from the space of the first large flow path 33a to the long first fine flow path 34a. Then, the blood sample or the like passes through the microgroove provided between the first microchannel 34a and the second microchannel 34b and flows into the second microchannel 34b. White blood cells, platelets and the like contained in a blood sample passing through the minute groove are observed. A blood sample or the like that has flowed from the second fine flow path 34 b to the second large flow path 33 b flows out of the specimen discharge hole 32. The resin microchannel array 3 is configured as described above.

  The transparent resin microchannel array 3 according to the present invention is formed by filling a mold with a transparent thermoplastic resin using a precision mold created by precision machine cutting / polishing, photolithography / electroforming, or the like as a mold. Can be obtained. Examples of the transparent thermoplastic resin include acrylic resins, cycloolefin resins, styrene resins, acrylic / styrene copolymers, polycarbonate resins, polyester resins such as polyethylene terephthalate, and ethylene / vinyl alcohol copolymers. Further, thermoplastic elastomers such as olefins, styrenes, and urethanes, vinyl chloride resins, fluorine resins, silicone resins such as polydimethylsiloxane, thermosetting resins, and photocurable resins can be used.

  In the present invention, since the microchannel array is made of resin, warpage occurs in the first substrate 36 depending on the storage state due to cooling and moisture absorption after molding, and the first substrate 36 and the transparent plate 5 are warped. A gap is created. That is, a gap is generated in the internal space between the first substrate 36 and the transparent plate 5 that becomes a flow path for a blood sample or the like. Thereby, a blood sample etc. will leak from this clearance gap. Therefore, as shown in FIGS. 1 and 2, the flow path forming region 38 is pressed by the rod-shaped structure 21 to force warping of the resin microchannel array 3. And the adhesiveness of the 1st board | substrate 36 and the transparent plate 5 improves at least in the perimeter of the flow-path formation area 38, and generation | occurrence | production of the clearance gap between the 1st board | substrate 36 and the transparent plate 5 can be suppressed. . That is, leakage of a liquid measurement sample such as a blood sample can be suppressed. Thereby, a liquid sample can be measured accurately.

  Next, embodiments of the present invention will be described with specific examples. The present invention will be specifically described based on examples, but the present invention is not limited to these examples.

  A method for producing the first jig 1 and the second jig 2 according to the present invention will be described. Since the jigs 1 and 2 are all manufactured by injection molding a resin, a mold was first manufactured by precision machining. And the resin-made jigs 1 and 2 were obtained by using the produced mold as a mold, filling the mold with an acrylic resin, and molding. The shape of the first jig 1 was such that the diameter of the recess was 27 mm, the observation hole was 11 mm in diameter, the wing portion 61 was 32 mm long × 32 mm wide × 2 mm thick, and the thickness other than the wing portion 61 was 1.5 mm. The height from the bottom surface of the wing 61 to the observation hole was 13 mm. The shape of the second jig 2 was such that the cross section of the rod-shaped structure 21 was 8 mm long × 16 mm wide, or 16 mm long × 16 mm wide. The height of the rod-shaped structure 21 was 9 mm, and the diameters of the sample introduction tube 22 and the sample discharge tube 23 were 1.6 mm. The wing portion 62 was 32 mm long × 32 mm wide × 2 mm thick.

  Next, a method for forming the resin microchannel array 3 will be described. First, a resist layer was formed by applying an organic material (resist) on a substrate. And the board | substrate with which the resist layer was formed and the mask processed into the desired mask pattern were aligned. Then, it exposed by irradiating UV light on a mask with the exposure apparatus suitable for a resist layer. And the exposed resist layer was developed with the developing solution, and the resist pattern was formed on the board | substrate. Further, if necessary, a resist layer was formed on the obtained resist pattern, and a series of steps of exposure and development with UV light was repeated.

Then, a conductive film was deposited on the surface of the substrate having the obtained resist pattern, applied to a nickel plating solution, and electroplated to obtain a metal structure (hereinafter referred to as Ni structure) on the resist pattern.
Furthermore, the obtained Ni structure was used as a mold, and acrylic resin was filled into the mold by injection molding to obtain a resin microchannel array 3.

  The entire outer shape including the flow path forming region 38 and the frame region 39 of the resin microchannel array 3 shown in FIG. 4 was 17 mm wide × 17 mm long × 1 mm thick. The depth of the grooves of the large flow paths 33a and 33b was 80 μm. Fifteen wall portions 35 that partition the recesses of the microchannels 34a and 34b were formed to a height of 80 μm. Further, each of the wall portions 35 has a shape having a total of 5100 fine grooves of 340 with a width of 10 μm and a depth of 5 μm. The diameter of the sample introduction hole 31 and the sample discharge hole 32 was 1.6 mm. Moreover, the area | region (flow-path formation area 38) which forms a groove | channel was 8 mm long x 16 mm wide. Further, water was absorbed into the resin microchannel array 3 to forcibly generate a warp of about 0.3 mm, and the resin microchannel array 3 was obtained.

  As shown in FIG. 3, the buffer rubber 4 pierces the EPDM sheet in a length of 8 mm × width of 16 mm, or a length of 16 mm × width of 16 mm, and at the same time pierces the specimen introduction path 41 and the specimen discharge path 42 to a diameter of 1.6 mm. Rubber 4 was obtained.

  Since the transparent plate 5 is produced by injection molding with a resin, a mold was first produced by precision machining. And the resin-made transparent board 5 was obtained by making the produced metal mold into a casting_mold | template, filling an acrylic resin in a casting_mold | template, and shape | molding. The shape of the transparent plate 5 was a disc shape, and had a diameter of 24 mm and a thickness of 1.5 mm (hereinafter abbreviated as transparent plate 5-A). Moreover, the transparent plate 5 in which a rectangular convex part was formed in the center part of the disk-shaped base | substrate as shown in FIG. 2 was also produced. In this case, the disk-shaped portion has a diameter of 24 mm and a thickness of 1.0 mm, and the rectangular convex portion has a length of 9 mm × width of 17 mm × thickness of 0.5 mm (hereinafter abbreviated as “transparent plate 5-B”).

The obtained first jig 1, second jig 2, resin-made microchannel array 3, buffer rubber 4, and transparent plate 5 were combined in the order shown in FIG. Then, with a weight of about 5 kg applied between the first jig 1 and the second jig 2, the wing part 61 of the first jig 1 and the wing part 62 of the second jig 2 are applied by vibration welding. And a test product was obtained.
As for the leakage state of the liquid sample, blood is injected from the specimen introduction tube 22 of the second jig 2 of the test article, and the presence or absence of leakage is observed at a magnification of 50 times with a real microscope and 500 times with a normal optical microscope. did.

Example 1.
The first jig 1, the second jig 2 whose cross section of the rod-shaped structure 21 is 8 mm long × 16 mm wide, the resin microchannel array 3 forcibly generating warpage of about 0.3 mm, 8 mm long × horizontal The 16 mm buffer rubber 4 and the transparent plate 5-B were combined in the order of FIG. 1 to produce a test product as shown in FIG. Further, the rod-shaped structure 21, the buffer rubber 4, and the transparent plate 5 -B are combined so that the flow path forming region 38 of the resin microchannel array 3 fits the rectangle. In the present embodiment, the contact surface of the buffer rubber 4 and the transparent plate 5-B with the resin microchannel array 3 is approximately the same size as the flow path forming region 38. The contact surface of the buffer rubber 4 with the rod-shaped structure 21 and the contact surface of the rod-shaped structure 21 with the buffer rubber 4 are also approximately the same size as the flow path forming region 38. Thereby, the flow path formation region 38 of the resin-made microchannel array 3 was effectively pressed to the first jig 1 side, and the warp of the resin-made microchannel array 3 was forced. As a result of introducing blood, no leakage was observed in all 5 sheets.

Example 2
The first jig 1, the second jig 2 whose cross section of the rod-shaped structure 21 is 8 mm long × 16 mm wide, the resin microchannel array 3 forcibly generating warpage of about 0.3 mm, 8 mm long × horizontal A 16 mm cushioning rubber 4 and a transparent plate 5-A were combined in the order of FIG. 1 to produce a test product as shown in FIG. Further, the rod-shaped structure 21 and the cushioning rubber 4 were combined so that the flow path forming region 38 of the resin microchannel array 3 fits the rectangle. In this embodiment, the contact surface of the buffer rubber 4 with the resin microchannel array 3 is approximately the same size as the flow path forming region 38. Further, the contact surface of the transparent plate 5-A with the resin microchannel array 3 is larger than the outer shape of the resin microchannel array 3 as a whole. The contact surface of the buffer rubber 4 with the rod-shaped structure 21 and the contact surface of the rod-shaped structure 21 with the buffer rubber 4 are approximately the same size as the flow path forming region 38. In the contact portion between the resin microchannel array 3, the transparent plate 5 -A and the buffer rubber 4, only the overlapping portion can be pressed by the rod-shaped structure 21. For this reason, also in the present embodiment, the flow path forming region 38 of the resin microchannel array 3 is effectively pressed to the first jig 1 side, and the warp of the resin microchannel array 3 is forced. As a result of introducing blood, no leakage was observed in all 5 sheets.

Example 3
The first jig 1, the second jig 2 whose cross section of the rod-shaped structure 21 is 16 mm long × 16 mm wide, the resin microchannel array 3 forcibly generating warpage of about 0.3 mm, 16 mm long × horizontal A 16 mm shock absorbing rubber 4 and a transparent plate 5-B were combined in the order of FIG. 1 to prepare a test product as shown in FIG. Moreover, it combined so that the rectangle of the transparent plate 5-B might fit in the flow-path formation area 38 of resin-made microchannel arrays 3. FIG. In the present embodiment, the contact surface of the buffer rubber 4 with the resin microchannel array 3 is larger than the flow path forming region 38 and is approximately the same size as the entire outer shape of the resin microchannel array 3. Further, the contact surface of the transparent plate 5-B with the resin microchannel array 3 is approximately the same size as the flow path forming region 38. The contact surface of the buffer rubber 4 with the rod-shaped structure 21 and the contact surface of the rod-shaped structure 21 with the buffer rubber 4 are larger than the flow path forming region 38, and the outer shape of the resin-made microchannel array 3 as a whole. Are approximately the same size. In the contact portion between the resin microchannel array 3, the transparent plate 5 -B, and the buffer rubber 4, only the overlapping portion can be pressed by the rod-shaped structure 21. For this reason, also in the present embodiment, the flow path forming region 38 of the resin microchannel array 3 is effectively pressed to the first jig 1 side, and the warp of the resin microchannel array 3 is forced. As a result of introducing blood, no leakage was observed in all 5 sheets.

Example 4
The first jig 1, the second jig 2 whose cross section of the rod-shaped structure 21 is 16 mm long × 16 mm wide, the resin microchannel array 3 forcibly generating warpage of about 0.3 mm, and the vertical 8 mm × The test rubber as shown in FIG. 7 was produced by combining the rubber 16 for buffering 16 mm wide and the transparent plate 5-B in the order of FIG. Further, the buffer rubber 4 and the transparent plate 5-B were combined so that the rectangular shape of the buffer rubber 4 and the flow path forming region 38 of the resin microchannel array 3 was matched. In the present embodiment, as in the first embodiment, the contact surface of the buffer rubber 4 and the transparent plate 5-B with the resin microchannel array 3 is approximately the same size as the flow path forming region 38. The contact surface of the buffer rubber 4 with the rod-like structure 21 is approximately the same size as the flow path forming region 38. In addition, the contact surface of the rod-shaped structure 21 with the buffer rubber 4 is larger than the flow path forming region 38 and is approximately the same size as the entire outer shape of the resin microchannel array 3. As a result, only the flow path forming region 38 of the resin-made microchannel array 3 was pressed toward the first jig 1, and the warp of the resin-made microchannel array 3 was forced. As a result of introducing blood, no leakage was observed in all 5 sheets.

Example 5 FIG.
The first jig 1, the second jig 2 whose cross section of the rod-shaped structure 21 is 8 mm long × 16 mm wide, the resin microchannel array 3 forcibly generating warpage of about 0.3 mm, 16 mm long × horizontal A 16 mm shock absorbing rubber 4 and a transparent plate 5-A were combined in the order of FIG. 1 to produce a test product as shown in FIG. In addition, the rod-shaped structures 21 were combined with the flow path forming region 38 of the resin microchannel array 3 so that the rectangles fit. In the present embodiment, the contact surface of the buffer rubber 4 with the resin microchannel array 3 is larger than the flow path forming region 38 and is approximately the same size as the entire outer shape of the resin microchannel array 3. The contact surface of the transparent plate 5-A with the resin microchannel array 3 is larger than the outer shape of the resin microchannel array 3 as a whole. The contact surface of the cushioning rubber 4 with the rod-shaped structure 21 is larger than the flow path forming region 38 and is approximately the same size as the entire outer shape of the resin-made microchannel array 3. Further, the contact surface of the rod-shaped structure 21 with the buffer rubber 4 is approximately the same size as the flow path forming region 38. In the contact portion between the resin microchannel array 3, the transparent plate 5 -A and the buffer rubber 4, only the overlapping portion can be pressed by the rod-shaped structure 21. Further, the contact surface of the rod-shaped structure 21 with the buffer rubber 4 is approximately the same size as the flow path forming region 38. Unlike the transparent plate 5 and the jigs 1 and 2, the buffer rubber 4 has low rigidity. For this reason, in this embodiment, only the portion of the resin-made microchannel array 3 corresponding to the contact portion between the rod-shaped structure 21 and the buffer rubber 4 among the overlapping portions is applied to the first jig 1 side. Work. For this reason, also in the present embodiment, the flow path forming region 38 of the resin microchannel array 3 is effectively pressed to the first jig 1 side, and the warp of the resin microchannel array 3 is forced. As a result of introducing blood, no leakage was observed in all 5 sheets.

Comparative Example 1
The first jig 1, the second jig 2 whose cross section of the rod-shaped structure 21 is 16 mm long × 16 mm wide, the resin microchannel array 3 forcibly generating warpage of about 0.3 mm, 16 mm long × horizontal A 16 mm cushioning rubber 4 and a transparent plate 5-A were combined in the order of FIG. 1 to produce a test product as shown in FIG. In this comparative example, unlike the above embodiment, all the contact surfaces are larger than the outer shape of the entire resin microchannel array 3. For this reason, the entire resin microchannel array 3 was pressed toward the first jig 1 side. Therefore, the flow path forming region 38 of the resin microchannel array 3 cannot be effectively pressed, and the warp of the resin microchannel array 3 is not forced. As a result of introducing blood, leakage was observed in 2 out of 5 sheets.

  Thus, the contact surface with the resin-made microchannel array 3 in the transparent plate 5, the contact surface with the resin-made microchannel array 3 in the buffer rubber 4, the contact surface with the rod-shaped structure 21 in the buffer rubber 4, And a common area 50 common when the contact surface of the rod-shaped structure 21 with the buffer rubber 4 is moved in the vertical direction with respect to the contact surface of the resin microchannel array 3 with the transparent plate 5, It is sufficient that at least the flow path forming region 38 is included and the resin microchannel array 3 is partially covered. That is, at least any one of all the contact surfaces may be approximately the same size as the flow path forming region 38 in a top view. Thereby, the warp of the resin-made microchannel array 3 is forced. Further, the leakage of the liquid measurement sample is reduced, and the liquid sample can be accurately measured.

  As described above, the common region 50 covers the entire region of the resin microchannel array 3 in the long side direction of the flow path forming region 38, and the flow path forming region in the short side direction of the flow path forming region 38. Although only 38 may be covered, it is not restricted to this. As long as at least the flow path forming region 38 can be effectively pressed and no gap is formed between the first substrate 36 and the transparent plate 5, the common region 50 may be determined in any way. For example, the common area 50 may be formed larger than the flow path forming area 38 in the short side direction of the flow path forming area 38, or the common area 50 is formed only in the flow path forming area 38 in the long side direction. It may be.

It is a disassembled perspective view which shows the structure of the microchannel array apparatus concerning embodiment. It is a schematic diagram which shows the structure of the microchannel array apparatus concerning embodiment. It is a schematic diagram which shows the structure of the rubber | gum for buffer concerning embodiment. It is a schematic diagram which shows the structure of the resin-made microchannel arrays concerning embodiment. FIG. 3 is a schematic diagram illustrating a configuration of a microchannel array device according to a second embodiment. FIG. 6 is a schematic diagram illustrating a configuration of a microchannel array device according to a third embodiment. FIG. 6 is a schematic diagram illustrating a configuration of a microchannel array device according to a fourth embodiment. FIG. 10 is a schematic diagram illustrating a configuration of a microchannel array device according to a fifth embodiment. It is a schematic diagram which shows the structure of the microchannel array apparatus concerning the comparative example 1. FIG.

Explanation of symbols

1 1st jig, 2nd jig, 3 resin microchannel array, 4 cushioning rubber,
5 transparent plate, 21 rod-shaped structure, 22 sample introduction tube, 23 sample discharge tube,
31 Sample introduction hole, 32 Sample discharge hole, 33a First large flow path, 33b Second flow path,
34a 1st microchannel, 34b 2nd microchannel, 35 wall part, 36 1st board | substrate,
38 channel formation region, 39 frame region, 41 sample introduction channel, 42 sample discharge channel,
50 common areas, 61 wings, 62 wings

Claims (5)

A microchannel array housing in which a resin microchannel array having a flow path forming region is incorporated,
A first jig having an observation hole;
A transparent plate that is formed larger than the observation hole and disposed on the non-viewing side of the observation hole;
A rubber cushion disposed on the side opposite to the first jig of the transparent plate with the resin microchannel array interposed therebetween,
A rod-shaped structure that is disposed on the opposite side of the buffer rubber from the resin microchannel array and presses the transparent plate, the resin microchannel array, and the buffer rubber toward the first jig side. A second jig,
Contact surface of the transparent plate with the resin microchannel array, contact surface of the buffer rubber with the resin microchannel array, contact surface of the buffer rubber with the rod-like structure, and the rod shape A common area common when the contact surface with the buffer rubber in the structure is moved in a direction perpendicular to the contact surface with the transparent plate in the resin microchannel array is at least the flow path formation. A microchannel array housing including a region and covering a part of the resin microchannel array.   2. The microchannel array housing according to claim 1, wherein the part of the resin-made microchannel array covered by the common region is a region that coincides with the flow path forming region or a region in the vicinity thereof.   3. The microchannel array according to claim 1, wherein a contact surface of the buffer rubber with the resin-made microchannel array coincides with the flow path formation region or a region near the flow path formation region. housing. The flow path forming region of the resin microchannel array is substantially rectangular,
The microchannel array housing according to any one of claims 1 to 3, wherein the common region covers the entire region of the resin microchannel array in the long side direction of the flow path forming region. The flow path forming region of the resin microchannel array is substantially rectangular,
The microchannel array housing according to claim 1, wherein the common area covers only the flow path forming area in a short side direction of the flow path forming area.

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