JP3126448B2 - Plasma collection circuit - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plasma collection circuit used for collecting plasma from a blood donor.

[0002]

2. Description of the Related Art Conventionally, a membrane-type plasma separator has been generally used as a plasma separator used in a plasma collection circuit. This membrane-type plasma separator usually has a separation membrane made of a hydrophobic material.In order to maintain the surface in a hydrophilic state and to facilitate a priming operation prior to plasma separation, the separator is used in advance. It is provided in a state where the liquid is filled therein. In a plasma collection circuit using such a membrane-type plasma separator, the entire circuit is usually filled with a liquid (Japanese Patent Laid-Open No. 2-5963).

[0003]

The plasma collection circuit needs to be sterilized, and is usually autoclaved. However, if autoclave sterilization is performed while the entire plasma collection circuit is filled with liquid, the filling liquid expands due to heating during sterilization, and the liquid from the end of the blood collection needle, anticoagulant introduction needle, priming liquid introduction needle, etc. Spilled and contaminated the needle tip. In the plasma collection circuit, many tubes are used to connect each member, for example, a blood collection needle to a blood storage bag, and a soft vinyl chloride resin tube is generally used as a tube. If autoclave sterilization is performed in a state where the liquid is filled in the soft vinyl chloride resin tube, additives such as a plasticizer and a lubricant in the vinyl chloride resin may be eluted into the liquid. Therefore, the present invention prevents the blood from leaking out of the blood collection needle and the like at the end of the plasma collection circuit and contaminating the blood collection needle, etc., even if the autoclave sterilization is performed. Even when only a portion is filled with liquid, even when many soft vinyl chloride tubes are used in the plasma collection circuit, elution of additives in the vinyl chloride resin during autoclave sterilization can be minimized. A plasma collection circuit is provided.

[0004]

Means for achieving the above object are as follows: a blood collection needle, a blood storage bag, a first tube communicating the blood collection needle with the blood storage bag, a plasma separator, and a waste liquid bag. A second tube communicating the blood cell component outlet of the plasma separator with the waste liquid bag, and a third tube communicating the plasma component outlet of the plasma separator with the waste liquid bag.
, A plasma bag, an anticoagulant introduction needle, a priming solution introduction needle, a fourth tube communicating the first tube with the blood inlet of the plasma separator, and the first tube A fifth connecting the second tube with the second tube
A tube, a sixth tube communicating the third tube and the plasma bag, a seventh tube communicating the anticoagulant introduction needle and the first tube, and the priming liquid introduction needle An eighth tube that communicates with the seventh tube, the fourth tube, and the fifth tube.
And a communicable sealing members provided respectively on the tube and the sixth tube, further, the plasma separation
Inside the vessel, inside the second tube, and inside the third tube.
Inside, the communicating sealing member of the fourth tube is
The inside of the plasma separator side portion from the provided position,
The fifth tube is provided with the communicable sealing member.
From the inside of the second tube side portion,
The sixth tube is provided with the sealing member capable of communicating with the sixth tube.
A plasma collection circuit in which a liquid is filled into the third tube side portion from a position where the plasma collection circuit is located.

[0005] The plasma collection circuit is preferably autoclaved. The said
1, the seventh tube, the eighth tube
And the inside of the blood storage bag is filled with liquid.
It is preferred that they are not present.

Therefore, embodiments of the present invention will be described with reference to the drawings. FIG. 1 shows a schematic diagram of one embodiment of the plasma collection circuit of the present invention. FIG. 2 shows one configuration of a communicable sealing member used in the plasma collection circuit of the present invention. FIG. 3 is an explanatory diagram for explaining an operation in a priming operation of the plasma collection circuit of the present invention.

The plasma collection circuit 1 of the present invention comprises a blood collection needle 10, a blood storage bag 12, a plasma separator 14, a waste liquid bag 16, a plasma bag 18, and an anticoagulant introduction needle 20.
A priming liquid introduction needle 22, a first tube 31 communicating the blood collection needle 10 with the blood storage bag 12, and a second tube 32 communicating the blood cell component outlet 14c of the plasma separator 14 with the waste liquid bag 16. A third tube 33 communicating the plasma component outlet 14b of the plasma separator with the waste liquid bag 16, and a fourth tube 34 communicating the first tube 31 with the blood inlet 14a of the plasma separator 14. And the first
Fifth connecting the first tube 31 and the second tube 32
Tube 35, third tube 33 and plasma bag 1
, A sixth tube 36 communicating the anticoagulant introduction needle 20 and the first tube 31, a priming solution introduction needle 22 and the seventh tube 3
An eighth tube 38 that communicates with the seventh tube 38 and sealing members 40, 41, and 42 that can be communicated with the fourth, fifth, and sixth tubes 34, 35, and 36, respectively.

The blood collection needle 10 is used to puncture a vein of a blood donor. The blood collection needle 10 is a known metal blood collection needle having a puncture needle at the tip capable of puncturing a vein of a blood donor. A needle or a resin-made blood collection needle is preferably used.

The blood storage bag 12 is for temporarily storing blood collected from a blood donor before collecting plasma, and the blood storage bag 12 is made of a soft synthetic resin (for example, a soft vinyl chloride resin). The closed blood storage container formed by the above is preferably used.

The plasma separator 14 is for separating blood collected in the blood storage bag 12 into a plasma component and a blood cell component. The plasma separator 14 flows the blood (whole blood) to be separated. Blood inlet 14a, a blood separating mechanism for separating the inflowing blood into plasma components and blood cell components, a blood cell component outlet 14c for discharging the separated blood cell components, and a plasma component outlet for discharging the separated plasma components 14b. As the plasma separator 14, a hollow fiber membrane type, a flat membrane type, or the like is suitably used.

[0011] The waste liquid bag 16 is placed in the plasma separator 1 before use.
4, for discarding the physiological isotonic solution and the priming solution filled in the interior of the second tube 32 and the third tube 33, etc., formed of a soft synthetic resin (for example, vinyl chloride resin) A sealed closed bag is preferably used. The waste liquid bag 16
May use two containers, a first waste liquid bag communicating with the second tube, and a second waste liquid bag communicating with the third tube.

[0012] The plasma bag 18 is
4 for storing the plasma flowing out from the plasma component outlet 14b and flowing out through the third tube 33 and the sixth tube 36. The plasma bag is formed of a soft synthetic resin. A bag-like material is preferably used.

The anticoagulant introduction needle 20 has an anticoagulant storage container 24 for injecting the anticoagulant into the blood storage bag 12.
It is for puncturing. This anticoagulant introduction needle 20
For example, a bottle needle or a hollow needle formed of metal or plastic can be preferably used. Further, the priming liquid introduction needle 22 is for puncturing the priming liquid storage container 26 so that the priming liquid flows into the blood component separation device. As the priming liquid introduction needle, a bottle needle, a hollow needle, or the like formed of metal or plastic can be suitably used.

In the blood component separation device 1 of the present invention, the blood collection needle 10 and the blood storage bag 12 are communicated by the first tube 31. Further, the blood cell component outlet 14 c of the plasma separator 14 and the waste liquid bag 16 are connected to the second tube 3.
It is communicated by 2. Further, the plasma component outlet 14 b of the plasma separator 14 and the waste liquid bag 16 are connected by a third tube 33. Further, the first tube 31 and the blood inlet 14 a of the plasma separator 14 are connected by a fourth tube 34. In this case, the first tube 3
The communication portion between the first and fourth tubes 34 has three or four
A connector with three ports may be used. A portion of the fourth tube 34 near the blood inlet 14a of the plasma separator 14 is provided with a sealing member
Is provided.

Further, the first tube 31 and the second tube 32 are connected by a fifth tube 35.
In this case, a connector having three ports may be used for a communication part between the first tube 31 and the fifth tube 35 and a communication part between the second tube 32 and the fifth tube 35. In addition, the second tube 35
A seal member 42 that can communicate is provided at a portion near the tube 32 side.

The third tube 33 and the plasma bag 1
8 is communicated with a sixth tube 36. In this case, a connector having three ports may be used as a communicating portion between the third tube 33 and the sixth tube 36. In addition, a seal member 41 that can communicate with the third tube 33 is provided at a portion of the sixth tube 36 near the third tube 33.

The sealing members 40, 41, 42 which can communicate with each other are:
FIG. 1 is for preventing the liquid filled in a specific portion (the interior of the plasma separator 14, the second tube 32, and the third tube 33) inside the blood component separation device 1 of the present invention from flowing out. , Are provided in the fourth, fifth, and sixth tubes 34, 35, and 36. As shown in FIG. 2, the communicating sealing members 40, 41, 42 are constituted by a soft tube 47 containing a breakable member 46 having a communicating portion 45. The communicable sealing members 40, 41, and 42 are in a closed state in a normal state, and are brought into a communicating state by breaking a portion 45 from the outside of the tube 47. The communicating sealing members 40, 41, 4
2 may be of a type in which each tube is pressed to seal the internal flow path, in addition to the one caused by breakage.

Further, the anticoagulant introduction needle 20 and the first tube 31 are connected by a seventh tube 37.
In this case, a connector having three or four ports may be used as a communicating portion between the first tube 31 and the seventh tube 37. In addition, the priming liquid introduction needle 22
The seventh tube 37 is communicated with an eighth tube 38. In this case, a connector having three ports may be used as a communicating portion between the first tube 31 and the seventh tube 37. In addition, the first tube 31
As the eighth tube 38, a transparent flexible synthetic resin tube such as soft vinyl chloride resin or silicone rubber is preferably used.

In the plasma collection circuit 1 of the present invention, the liquid such as RO water is filled in the plasma separator 14, the waste liquid bag 16, and the second tube 32 and the third tube 33 in advance. Have been. Further, it is preferable that the entirety of the second tube 32 and the third tube 33 is filled with liquid. It is sufficient that at least a certain amount of liquid is filled at the connection side of each of the tubes 32 and 33 with the plasma separator 14. Further, the inside of the plasma separator 14 side from the position where the fourth tube 34 is provided with the communicable sealing member 40 and the fifth tube 35 communicable sealing member 42 are provided. The liquid is also filled into the inside of the portion on the second tube 32 side from the position and the inside of the portion on the third tube 33 side from the position where the sealing member 41 capable of communicating with the sixth tube 36 is provided. Is preferred. Further, the waste liquid bag 16 may be filled with some liquid. The plasma collection circuit 1 is sterilized in an autoclave in a state where the above-described portion is filled with a liquid. Then, at least the first tube 31, the sixth tube 36, the seventh tube 37, the eighth tube 38 and the blood storage bag 1
The inside of 2 is not filled with liquid. Therefore, in the plasma collection circuit 1, the plasma separator 14, the waste liquid bag 16
The liquid is filled only inside the tubes 32 and 33 that communicate the fluid and the plasma separator 14, and the filled liquid is
The third sealing members 40, 41, and 42 are configured so as not to flow out to other portions. Further, since the inside of the plasma separator 14 is filled with the liquid as described above, even if the plasma separation membrane to be used is formed of a hydrophobic material, the surface is maintained in a hydrophilic state, Further, the priming operation for removing bubbles before use is facilitated. Then, the plasma separator 14 is connected to the tubes 32, 3 as described above.
3 communicates with the waste liquid bag 16 so that the plasma separator 14 expands by heating during autoclave sterilization.
In addition, it is possible to absorb the expansion of the liquid in the tubes 32 and 33.

Further, in the plasma collection circuit 1 of the present invention,
When the blood and the anticoagulant liquid in the blood storage bag 12 flow into the plasma separator 14 with a head, the first tube 31 and the seventh tube 37 are long enough to ensure a head with the plasma separator 14. It is preferable that the first tube 31 is about 100 cm, and the seventh tube is about 70 cm.

[0021]

The operation of the plasma collection circuit of the present invention in the priming operation will be described with reference to FIGS. First,
The priming liquid storage container 26 storing a priming liquid such as physiological saline is placed above the blood storage bag 12 by 80c.
m, the priming liquid introduction needle 22 is punctured into the priming liquid storage container 26 after closing the forceps 51 of the eighth tube 38. Next, the first tube 31
The forceps 52 of the third tube 33, the forceps 53 of the third tube 33, and the forceps 54 of the second tube 32 are closed respectively, and the communicable sealing members 42 provided on the fourth tube 34 and the fifth tube 35 are manually closed. To make the liquid flowable. Then, the forceps 51 are opened, and the priming solution 20 is opened.
After allowing g to flow into the blood storage bag 12, the forceps 51 are closed. A part of the priming solution 20g flows into the blood storage bag 12 and the anticoagulant introduction needle 20 through the eighth tube 38 and the seventh tube 37. In this way, the priming of the anticoagulant introduction needle 20 is performed. Next, when the forceps 52 of the first tube 31 is opened, the priming solution (the remainder of 20 g of the priming solution) in the blood storage bag 12 flows through the first tube 31 so as to push out air, and And into the waste bag 16 through the second tube, and the other into the blood collection needle 10. Thus, the priming operation of the blood collection needle 10 is performed. And then, the forceps 55 of the first tube 31
And 52 are closed. Next, open the forceps 51 again,
Further, the forceps 53 and 54 are opened, and the priming solution 4
After flowing 00 g into the blood storage bag, the forceps 51 are closed. Here, the priming solution that has flowed flows through the eighth, seventh, and fourth tubes 38, 37, and 34, flows into the plasma separator 14, and then flows into the second and third tubes 32,
The liquid flows into the waste liquid bag 16 through 33. Thereafter, the eighth tube 38 and the second and third tubes 32, 3
The waste liquid bag 16 side of No. 3 is sealed using a high frequency sealing device or the like, and the priming liquid introduction needle 22 and the waste liquid bag 16 are separated from the circuit. As described above, the priming operation of the plasma collection circuit of the present invention is completed except for the inside of the sixth tube 36 which does not require priming.

[0022]

According to the plasma collection circuit of the present invention, there are provided a blood collection needle, a blood storage bag, a first tube connecting the blood collection needle and the blood storage bag, a plasma separator, a waste liquid bag, and the plasma separation bag. A second tube communicating the blood cell component outlet of the blood vessel with the waste liquid bag, a third tube communicating the plasma component outlet of the plasma separator with the waste liquid bag, a plasma bag, an anticoagulant An introduction needle, a priming liquid introduction needle, a fourth tube communicating the first tube with the blood inlet of the plasma separator, and a fourth tube communicating the first tube with the second tube. A fifth tube, a sixth tube communicating the third tube with the plasma bag, a seventh tube communicating the anticoagulant introduction needle with the first tube, and the priming solution introduction Needle and the seventh An eighth tube communicating the cube, the fourth tube, the fifth tube and before
And a communicable sealing member provided on each of the sixth tubes , further comprising:
Inside the tube, inside the third tube, inside the fourth tube
The position where the sealing member capable of communicating with the tube is provided.
The inside of the plasma separator side portion from the position, the fifth tube
From the position where the communicable sealing member is provided.
Inside the second tube side portion, the sixth tube
Before the position where the communicable sealing member is provided.
Since the inside of the third tube side is filled with liquid, during autoclave sterilization, liquid is prevented from flowing out from the blood collection needle at the end of the plasma collection circuit and contaminating the blood collection needle. Further, even when many soft vinyl chloride tubes are used in the plasma collection circuit, elution of additives from the vinyl chloride resin during autoclave sterilization can be reduced as much as possible.

[Brief description of the drawings]

FIG. 1 is a schematic diagram showing one embodiment of a plasma collection circuit of the present invention.

FIG. 2 is a cross-sectional view of a communicable sealing member used in the plasma collection circuit of the present invention.

FIG. 3 is an explanatory diagram for explaining an operation in a priming operation of the plasma collection circuit of the present invention.

[Explanation of symbols]

 Reference Signs List 10 blood collection needle 12 blood storage bag 14 plasma separator 16 waste liquid bag 18 plasma bag 20 anticoagulant introduction needle 22 priming liquid introduction needle 24 anticoagulant storage container 26 priming liquid storage container 31 first tube 32 second tube 33 Third tube 34 Fourth tube 35 Fifth tube 36 Sixth tube 37 Seventh tube 38 Eighth tube 40 Communicable sealing member 41 Communicable sealing member 42 Communicable sealing member

Claims (3)

(57) [Claims] 1. A blood collection needle, a blood storage bag, a first tube communicating the blood collection needle with the blood storage bag, a plasma separator, a waste liquid bag, a blood cell component outlet of the plasma separator, and A second tube communicating with the waste liquid bag, a third tube communicating the plasma component outlet of the plasma separator with the waste liquid bag, a plasma bag, an anticoagulant introduction needle, and a priming liquid introduction needle A fourth tube communicating the first tube with a blood inlet of the plasma separator; a fifth tube communicating the first tube with the second tube; A sixth tube for communicating the tube with the plasma bag, a seventh tube for communicating the anticoagulant introduction needle and the first tube, a priming solution introduction needle and the seventh tube, Eighth communicating with A tube, the fourth tube,
And a said fifth tube and the sixth communicable sealing member respectively provided tube, further, before
The inside of the plasma separator, the inside of the second tube, and the third
Inside the tube, the fourth tube can communicate with the
The plasma separator side portion from the position where the sealing member is provided
Inside the minute, the communicable seal of the fifth tube
From the position where the material is provided to the second tube side portion
Inside, the sealing member capable of communicating with the sixth tube
Of the third tube side portion from the position where
A plasma collection circuit having a liquid filled inside . 2. The plasma collection circuit according to claim 2, wherein
2. The method according to claim 1, wherein the plasma is collected.
Road. 3. The first tube and the seventh tube.
Inside the eighth tube and the blood storage bag.
Is an unfilled liquid or claim 1 or
3. The plasma collection circuit according to 2.