JP3122001U - Mobile phase liquid container - Google Patents

Abstract

The present invention provides a mobile phase liquid container that enhances the degassing effect of a mobile phase liquid that has been degassed by a vacuum degassing method using an aspirator.
In a mobile phase liquid container of a liquid chromatograph, a brush-like instrument 6 having cilia 61 is mounted at a position where the cilia 61 is immersed in the mobile phase liquid. Thereby, when solubility changes, since a bubble will be formed in the surface of the cilia 61 and deaeration will be accelerated | stimulated, the deaeration effect will continue longer than before. The formed bubbles may be removed by a method such as applying an appropriate vibration by the operator.
[Selection] Figure 1

Description

  The present invention relates to a mobile phase liquid container suitable as a mobile phase liquid reserve tank used in, for example, a liquid chromatograph.

In liquid chromatography, the mobile phase liquid is degassed before use. This prevents air dissolved in the mobile phase liquid from becoming bubbles and lowering the pumping accuracy, and causing adverse effects such as noise and baseline fluctuations on the detector, and performs stable analysis. Because. There are several methods for degassing, roughly divided into online methods and offline methods. The on-line method is a method of deaeration in the process of sending a mobile phase liquid, and a bubbling method using helium gas or a vacuum membrane deaeration method using a gas-liquid separation membrane is widely used. The reduced-pressure membrane deaeration method is a method in which a mobile phase liquid is passed through a tube made of a gas-liquid separation membrane placed under reduced pressure, and dissolved gas is discharged through the wall of the tube (for example, patents). Reference 1). In contrast, the off-line method is a method in which the mobile phase liquid is degassed in advance before analysis, and the vacuum degassing method using an aspirator is often used as the simplest method.
JP 2000-107510 A

Among the above degassing methods, the bubbling method using helium gas consumes helium gas and thus requires an operating cost. In addition, the reduced-pressure membrane deaeration method is expensive. On the other hand, the vacuum degassing method using an aspirator is simple and does not cost, but the degassing effect is not sustained because re-dissolution of air occurs immediately after the degassing process is completed.
The present invention has been made in view of such circumstances, and an object thereof is to provide a mobile phase liquid container that enhances the sustainability of the degassing effect of the mobile phase liquid processed by the vacuum degassing method using a simple aspirator. And

  In order to solve the above-mentioned problems, the present invention mounts a brush-like instrument having cilia in a mobile phase liquid container of a liquid chromatograph at a position where the cilia is immersed in the mobile phase liquid. As a result, bubbles are formed on the surface of the cilia to promote deaeration, so that the deaeration effect lasts longer than before.

  Since this invention is comprised as mentioned above, even if it performs the deaeration process by the off-line method which is simple and does not cost, the effect lasts long.

The mobile phase liquid container provided by the present invention is characterized in that a brush-like instrument having cilia is mounted so as to be immersed in the liquid in the container.
Therefore, the basic configuration of the best mode is a mobile phase liquid container so configured.

FIG. 1 shows an embodiment of the present invention.
In the figure, reference numeral 1 denotes a container body which is a main component of the mobile phase liquid container 10, and the mobile phase liquid 2 is filled therein. Reference numeral 3 denotes a liquid feeding tube connected to a liquid feeding pump (not shown) of the liquid chromatograph, and a suction filter 31 for preventing mixing of solid foreign matters is attached to the tip of the tube. Reference numeral 4 denotes a lid of the container body 1, through which the liquid feeding tube 3 passes, and a vent hole 41 is provided thereon. Reference numeral 6 denotes a brush-like instrument configured by attaching the cilia 61 to the support column 62, and is detachably attached to the back of the lid 4 at the support column 62 portion. When the mobile phase liquid 2 is filled in the container main body 1, the cilia 61 is immersed in the mobile phase liquid 2. The material of the brush-like instrument 6 (including the cilia 61) is polypropylene or polyethylene that is insoluble in common organic solvents (such as methanol and acetonitrile) used as a mobile phase in a liquid chromatograph.

The apparatus of this embodiment configured as described above operates as follows.
In general, the reason why gas is generated in the mobile phase liquid is a change in the solubility of the gas caused by a change in the outside air temperature or a mixing of the solvent. When the solubility of the gas dissolved in the liquid changes for some reason, the existence state becomes unstable, and it tends to form bubbles on the surface of an object in the vicinity. Utilizing this property, deaeration is performed by forming bubbles on the surface of the cilia 61 when the solubility changes. The formed bubbles may be removed by a method such as applying an appropriate vibration by the operator.

FIG. 2 shows an example of the usage procedure of the apparatus of this embodiment. The procedure for using the apparatus of this embodiment will be described below with reference to FIG. The following items (1), (2)... Correspond to (1), (2).
(1) For example, water and methanol are mixed in the container body 1 as the mobile phase liquid 2 at a predetermined mixing ratio.
(2) Cover the lid 4, connect the vent hole 41 and the aspirator 5 with the decompression tube 7, operate the aspirator 5 while stirring with the stirrer 8, and perform degassing under reduced pressure.
(3) After the deaeration is completed, the brush-like instrument 6 is attached to the back surface of the lid 4 so that the cilia 61 are immersed in the liquid.
(4) The liquid pump 9 is operated to perform liquid chromatographic analysis. When bubbles are attached to the cilia 61 during analysis, they are removed by a method such as applying vibrations as appropriate.

  Instead of attaching / detaching the brush-like instrument 6 to / from the back surface of the lid 4, the lid 4 to which the brush-like instrument 6 is fixed and the lid 4 without the brush-like instrument 6 are prepared and exchanged. Also good. Further, the brush-like instrument 6 does not necessarily have to be attached to the lid 4, and a configuration in which the brush-like instrument 6 is attached to the container body 1 is also possible.

  The present invention can be used for a liquid chromatograph.

It is a figure which shows one Example of this invention. It is a figure which shows the use procedure of this invention apparatus.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Container body 2 Mobile phase liquid 3 Liquid supply tube 4 Lid 5 Aspirator 6 Brush-like instrument 7 Depressurization tube 8 Stirrer 9 Liquid feed pump 10 Mobile phase liquid container 31 Suction filter 41 Ventilation hole 61 Cilia 62 Strut

Claims (1)

  A mobile phase liquid container for storing a mobile phase liquid in a liquid chromatograph, wherein a brush-like instrument having cilia is attached to a position where the cilia is immersed in an internal mobile phase liquid.

Images