JP3117964B2 - Antitumor substance - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a substance derived from a tumor cell.
Related to antitumor substances synthesized by germs
is there.

[0002]

2. Description of the Related Art Conventional anti-pulmonary substances are chemically synthesized.
Or an extract from a non-tumor organism. Tumor and tumor production
Report on the synthesis of antitumor substances by decomposing living organisms with fungi
There is no notice yet.

[0003]

SUMMARY OF THE INVENTION The present inventor has concluded the research.
As a result, a substance derived from a tumor cell (antitumor substance = tumor cell denatured substance)
Is degraded by a bacterium to produce antitumor
Synthetic substance = Synthesizes a tumor cell degenerating substance, and the molecular structure of this substance
Clarified.

[0004] The present invention utilizes this research result.
The challenge is to convert the substance derived from tumor cells
Antitumor substance that causes degeneration in tumor cells synthesized more
Is to provide.

[0005]

According to the first aspect of the present invention, a tumor
According to the following structural formula synthesized by cell spirits from cell-derived substances
Characterized in that it is an antitumor substance.

[0006] Structural formula

Embedded image

[0007]

EXAMPLES In this example, DDY mice and Ehrlich tumor cells were used. Extracted from material from tumor cells (anti-tumor substance = underlying material of tumor cells modified material synthesis)
Discharge was processed in the following order. (1) Tumor cell culture ascites (500 ml was defined as 1 unit)
After heat treatment (causing heat denaturation of tumor cells), coagulation and extraction were performed at 80% ethyl alcohol concentration.
Toyo filter paper No. Filter through 2. (2) The water-insoluble substance precipitated after the alcohol was removed by evaporation was used as filter paper No. Filter off at 2. (3) After confirming complete removal of the alcohol, acetone was added, and the mixture was treated with acetone at a concentration of 80%. Filter through 2. (4) After removing acetone by evaporation, add chloroform and shake well. The chloroform layer is separated by a separation funnel, chloroform is removed by evaporation, and distilled water is added to prepare an aqueous solution. (5) After adding ether to the aqueous solution and shaking well, the aqueous layer is separated and concentrated. (6) Separate the concentrated solution by a paper chromatography method (descent method, developing solution: butanol: glacial acetic acid: water = 4: 1: 2),
A ninhydrin reaction-positive substance is obtained in the range of Rf = 0.25-0.3. (This is an antitumor substance derived from a tumor cell = a substance that is the basis of the synthesis of a tumor cell degenerating substance.) (7) The method of confirming an antitumor substance includes the substance identified and extracted in the above (6). NaCl 0.5%, glucose 1
%, 40 mg of ATP, pH 6.8-7.0, and cultivation of a bacterium (Bacteria) at 36.5 ° C. for 5 days.
(Reason for 5 days of cultivation: From the 5th day after the start of cultivation, glucose is decomposed by spirit bacteria to lower the pH and the decomposition / synthesis is stopped.) After cultivation, sterile filtration is performed, and then ethyl is added. After treatment with alcohol at a concentration of 80%, filtration and removal of the alcohol by evaporation . Thereafter, the mixture is treated with acetone at a concentration of 80%, filtered, and the acetone is removed by evaporation. Then, chloroform is added and the mixture is shaken to separate and separate the chloroform layer . Chloroform isolated
After evaporating off chloroform, distilled water is added to the layer to form an aqueous solution. Ether is added to this aqueous solution, and after shaking well, the aqueous layer is separated and concentrated to obtain a concentrate. The concentrate was separated by the above-mentioned paper chromatography method, and Rf = 0.13-0.1
Ninhydrin reaction positive substances (anti-tumor substances =
Tumor cytopathic material). Extract this fraction, below
Purified by high performance liquid chromatography under the indicated measurement conditions ,
It was concentrated to about 2 ml . The concentrate in tumor cells (Ehrlich tumor cells) transplanted subcutaneously or intra-abdominal vagina mouse abdominal vagina, from tumor cells abdominal vaginal implantation day 3, each 0.5ml once every 3 days was administered. On day 10 or day 12 after tumor cell transplantation, ascites was collected and degeneration of tumor cells was confirmed by Giemsa staining. This
As shown in the photomicrographs of FIGS. 1 and 2, it was confirmed that the cytoplasm that caused the degeneration of the tumor cells was leaked . Decomposition of the nucleus was also observed. The mechanism of action of the antitumor substance (tumor cell degenerating substance) presented here is thought to be due to degeneration of the tumor cells, which impairs the repair of the tumor cell wall after separation of daughter cells, leading to degradation of the tumor cells. (8) The molecular weight and molecular structure of the synthesized antitumor substance are as follows:
The structure was analyzed by NMR and MS, and the following results were obtained. Although this structure does not contain nitrogen, the ninhydrin coloring observed on the order of the paper chromatography method is considered to be formamide (NH ₂ CHO) coexisting. In addition, this structural formula is recognized as a molecular weight of 196 from the result of mass spectrometry (MS).

[0008] Structural formula

Embedded image

[0009]

According to the present invention, a substance derived from a tumor cell
Provided an anti-tumor substance of the above structural formula synthesized by psychiatric fungi
Is done.

[Brief description of the drawings]

FIG. 1 is a photomicrograph (copy) of a degenerated tumor cell.

FIG. 2 is a photomicrograph (copy) of nucleated cells.

Claims (1)

(57) [Claims] 1. Synthesizing a substance derived from a tumor cell by a psychiatric fungus
An antitumor substance having the following structural formula: Structural formula [ Formula 1]