JP3112794B2 - Plaque formation inhibitor - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plaque formation inhibitor, which is a food or oral product containing an extract from cacao which has been subjected to alkali treatment and has a plaque formation inhibitory effect, or a decomposition product thereof.

[0002]

2. Description of the Related Art In caries, sucrose in food is converted into sticky glucan by glucosyltransferase produced by Streptococcus genus, which is one of the causative bacteria, and this glucan adheres to the tooth surface. The prevailing theory is that the plaque is formed and the enamel is destroyed by the pH of the tooth surface being reduced by the organic acids produced by the microorganisms growing in the plaque. Therefore, as a method of suppressing caries, a method of suppressing plaque formation, a method of suppressing acid production by bacteria in plaque, and a method of decomposing and removing generated plaque have been proposed.

Conventionally, some substances have already been proposed by searching for a plaque formation inhibitory substance for suppressing plaque formation. Above all, material development from edible materials which humans have been edible for a long time has been actively promoted.For example, Japanese Patent Application Laid-Open No. 61-260017 discloses an oral cavity containing gamma-linolenic acid or linoleic acid which has an effect of preventing dental caries. The composition is
Japanese Patent Application Laid-Open No. 1-9922 discloses an anti-cariogenic and anti-periodontal disease composition containing catechins obtained from green tea.
Discloses a method for producing an anti-cariogenic substance extracted from oolong tea and its use.

As for the cocoa component, an oral composition using an extract extracted from cocoa husk (Japanese Patent Laid-Open Publication No.
01614) and the effect of water extract from defatted cocoa powder [A. Stralfors, Archs oral Biol., 11, 141-161, 1966
Year]. Also, a method for treating cacao beans by extracting a hot water-soluble portion from cacao mass subjected to alkali treatment using an alkali agent has been reported (JP-A-59-169452). Has not been reported at all.

[0005] In addition to safety, a plaque formation inhibitory substance used as a food or an oral product needs to be excellent in food processability and excellent in taste. Further, those which are difficult to extract from raw materials or require complicated extraction and production steps are disadvantageous in terms of cost and are not industrially suitable. Conventional plaque formation inhibitors do not sufficiently satisfy these requirements, and there is a demand for a less expensive and more effective plaque formation inhibitor and a plaque formation inhibitor using the same.

[0006]

DISCLOSURE OF THE INVENTION The present invention relates to an excellent plaque containing a plaque-forming inhibitory substance which is rich in safety and processability, has good taste, and can be efficiently extracted from an inexpensive material. It is intended to provide a formation inhibitor.

[0007]

According to the present invention, an extract extracted with a polar solvent from cocoa mass treated with alkali using an alkali agent or cocoa powder obtained by squeezing oil from the cocoa mass, or a decomposition product thereof is used. A plaque formation inhibitor characterized by comprising:

In the invention having the above constitution, the method of extracting with cacao mass treated with alkali or cocoa powder obtained by squeezing the cacao mass with a polar solvent is basically carried out according to the method of JP-A-59-169452. be able to. That is, the alkali-treated cocoa mass is immersed in an alkali agent, preferably an aqueous solution of an alkali agent such as sodium, potassium, magnesium, ammonium or the like, a bicarbonate or a hydroxide, in the roasted cocoa beans or cocoa nibs. After drying and grinding. Or
It can be obtained by immersing the aqueous solution of the alkaline agent in unbaked cocoa beans or cocoa nibs, and then baking and grinding. Cocoa powder can also be obtained by partially crushing cacao mass obtained by grinding and then grinding.

The cocoa mass or the cocoa powder thus obtained is added with a polar solvent, preferably water or a hydrous alcohol, optimally at a temperature of 70 to 130 ° C., 3 to 10 times the amount of hot water to extract the soluble part. To obtain an extract. In addition, the decomposition product is acid, alkali,
A decomposition product obtained by the action of an enzyme, preferably an acid such as dilute hydrochloric acid, dilute sulfuric acid, or an organic acid, an alkali such as sodium hydroxide, potassium hydroxide, or sodium carbonate, or a protease, dextranase, or hemihydrate. It can be obtained by decomposing by the action of enzymes such as cellulase, phenolase, tyrosinase and tannase.
As the decomposition product of the present invention, a plaque formation inhibitory action obtained by fractionating the decomposition product obtained as described above by a conventional operation using a commercially available ion exchange resin, column chromatography, or the like. Also included are fractions with strong

[0010] The obtained extract or decomposed product is pH 6-8
After adjusting to, desalting and concentrating, as a liquid substance, or, further, by adding a spray-drying and the like and dried to obtain a plaque formation inhibitory substance as a solid substance, containing this substance Thereby, an effective plaque formation inhibitor can be provided. Examples of such a plaque formation inhibitor include a food or an oral product containing the extract or degradation product of the present invention, and preferably, the extract of the present invention.
Food products such as chocolate, gum, candy, tablets, cookies, cocoa, beverages, ice cream, etc., or oral products such as toothpastes or mouthwashes containing 0.01 to 10%.

[0011] Plaque is formed by converting sucrose in food into sticky glucan by glucosyltransferase produced by Streptococcus mutans and accumulating it on teeth. The extract extracted with a polar solvent from cocoa mass treated with alkali or cocoa powder obtained by squeezing the cocoa mass with a polar solvent or its decomposed product remarkably causes adhesion and accumulation (plaque formation) of glucan to teeth by Streptococcus mutans. Shows inhibitory action. Therefore, the sucrose in the plaque formation inhibitor containing the extract or the degradation product of the present invention can move from the oral cavity to the digestive tract as it is without being affected by the plaque formation of Streptococcus mutans. .

[0012] Cocoa mass is an inexpensive food material that has been used for foods since ancient times, and an extract or its decomposition product that can be extracted more efficiently than cacao mass has high safety, taste,
It has good properties in terms of food processing suitability.

[0013]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to the following examples. First, a method for preparing an extract or a decomposition product extracted from cocoa mass subjected to alkali treatment using an alkali agent or cocoa powder obtained by squeezing oil from the cocoa mass is described, and then plaque formation of these extracts or decomposition products is shown. An inhibition test is shown, and then a specific formulation of the plaque formation inhibitor of the present invention is shown as an example.

[0014]

Example 1 Preparation method of alkali-treated cacao mass extract 1.6% by weight of potassium carbonate and 25% by weight of water were added to cocoa nibs peeled and crushed, and alkali-treated while mixing at 90 ° C. for 1 hour, followed by roasting. 500 kg of hot water at 70 ° C. was added to 100 kg of cacao mass of pH 7.0 obtained by baking and grinding, and mixed and stirred while keeping the temperature at 70 ° C. in a colloid mill.

Next, using a continuous solid-liquid separator, centrifugal force of 250
Separate continuously at 0G, and a mixture of water-soluble part and fine particle part 3
80 kg, cocoa butter 60 kg and extraction residue 160 kg were obtained. The mixture of the water-soluble part and the fine particle part was concentrated under reduced pressure at 70 ° C. and 180 Torr to obtain a concentrated liquid having a solid content of 50% by weight. The concentrate
It was dried continuously at 0.3 Torr and pulverized to obtain a cocoa mass extract with a water content of 1.5% by weight. The cocoa mass extract had a yield of 21% by weight based on the cocoa mass.

[0016]

Example 2 Preparation method of alkali-treated cocoa powder extract Peeled cocoa beans, 2.5% by weight of potassium bicarbonate and 30% by weight of water were added to crushed cocoa nibs, alkali-treated at 90 ° C. for 1 hour, and roasted. The baked and ground cacao mass was squeezed to obtain a cocoa cake having an oil content of 22% by weight, which was pulverized to obtain a cocoa powder. To 100 kg of the cocoa powder, 600 kg of hot water at 100 ° C. and 2 kg of ethyl alcohol for food addition were added, and the mixture was formed into a colloid using a high-pressure homogenizer, and separated at a centrifugal force of 3200 G using a cage centrifuge. The portion having the water-soluble portion, the fine particle portion, and the cocoa butter was centrifuged again to separate the cocoa butter from the mixture of the water-soluble portion and the fine particle portion. The mixture was powdered in the same manner as in Example 1 to obtain a cocoa powder extract having a water content of 2.5% by weight.

[0017]

Example 3 Preparation of Acid Decomposed Product 20 g of the alkali-treated cocoa mass extract obtained in Example 1 and 1000 ml of 1N sulfuric acid were placed in a 2000 ml Erlenmeyer flask,
After heat treatment at 5 ° C. for 6 hours, filtration with filter paper was performed. The obtained filtrate was neutralized with an aqueous sodium hydroxide solution, further desalted by electrodialysis, concentrated under reduced pressure, and lyophilized to obtain 9.0 g of an acid decomposition product.

[0018]

Example 4 Method for Preparing Fractions 9.0 g of the acid hydrolyzate obtained in Example 3 was dissolved in 100 ml of water, and a strongly acidic cation exchange resin (Amberlite 200C,
Column packed with Rohm and Haas (US 2.0)
× 30 cm) and adsorbed. The fraction not adsorbed to the resin and the washing solution washed with 200 ml of water after the adsorption operation were combined to obtain a non-adsorbed fraction. This fraction was neutralized with an aqueous sodium hydroxide solution, further desalted by electrodialysis, concentrated under reduced pressure, and lyophilized to obtain 8.2 g of a fraction.

[0019]

Example 5 Preparation of Alkali Decomposed Product 20 g of the alkali-treated cacao mass extract obtained in Example 1 and 1000 ml of 1N aqueous sodium hydroxide solution were placed in a 2000 ml Erlenmeyer flask, heated at 95 ° C. for 6 hours, and then filtered. Filtration. The obtained filtrate was neutralized with hydrochloric acid,
Further, the solution was desalted by electrodialysis, concentrated under reduced pressure, and freeze-dried to obtain 10.2 g of an alkaline decomposition product.

[0020]

Example 6 Method for Preparing Decomposed Product Using Hemicellulase 20 g of the alkali-treated cacao mass extract obtained in Example 1 was used.
Place in a 300 ml Erlenmeyer flask and add 50 mM phosphate buffer (pH 6.
5) Add 200ml and dissolve. Hemicellulase (Sigma)
Was added and reacted at 37 ° C. for 18 hours. Stop the reaction by heating at 100 ℃ for 10 minutes, concentrate under reduced pressure, and freeze-dry.
8.7 g of a hemicellulase degradation product was obtained.

[0021]

Example 7 Plaque Formation Inhibition Test Alkali-treated cacao mass extract obtained in Example 1, alkali-treated cocoa powder extract obtained in Example 2, Example 3
The plaque formation inhibition test was performed on the acid digest obtained in the above, the fraction obtained in Example 4, the alkaline digest obtained in Example 5, and the hemicellulase digest obtained in Example 6 as follows. I went to.

Brain Heart Infusion medium containing 1.0% sucrose and 0.5% of each test sample 1
0 ml was added to a glass test tube, a glass rod of the same length as the test tube was put therein, and Streptococcus mutans MT8148 strain previously cultured at 37 ° C for 18 hours in a Brain Heart Infusion medium without sucrose. Broth 10
0 μl was added. After stationary culture at 37 ° C. for 24 hours, the glass rod was transferred to the above medium, and further cultured at 37 ° C. for 24 hours, and the amount of plaque formed on the glass rod (A) was measured by the phenol sulfate method. Plaque amount formed when no test sample is added (B)
Table 1 shows the results obtained by determining the inhibition rate (%) by the following formula using this as a control.

Inhibition rate (%) = [(BA) × 100] ÷ B From Table 1, in the plaque formation test performed by adding the extracts of Examples 1 to 6 or the decomposition products thereof, Streptococcus
It can be seen that the conversion of sucrose to adherent glucan by the enzyme produced by mutans was suppressed, and as a result, the amount of plaque formed on the glass rod was suppressed.

[0024]

[Table 1] Test sample Inhibition rate (%) Cocoa mass extract 50 Cocoa powder extract 52 Acid digest 62 Fraction 70 Alkaline digest 60 Hemicellulase digest 55

[0025]

Example 8 Chocolate Cocoa Mass 20 Cocoa Butter 19 Sugar 49.4 Powder Milk 6.0 Lecithin 0.6 Cocoa Mass Extract * 5.0 Total 100 (wt%) * Alkali-treated cacao mass extract obtained in Example 1

[0026]

[Example 9] Gum Gum base 20 Sugar 55 Glucose 15 Ginger syrup 14.99 Acid hydrolyzate * 0.01 Total amount 100 (wt%) * Acid hydrolyzate obtained in Example 3

[0027]

[Example 10] Candy sugar 50 starch syrup 43 water 2.O Hemicellulase hydrolyzate * 5.0 Total amount 100 (wt%) * Hemicellulase hydrolyzate obtained in Example 6

[0028]

Example 11 Cocoa Cocoa Powder 38.5 Powdered Sugar 35 Glucose 16 Powdered Milk 5.5 Cocoa Powder Extract * 5.0 Total 100 (% by Weight) * Alkali-treated Cocoa Powder Extract Obtained in Example 2

[0029]

Example 12 Dentifrice Calcium phosphate dibasic 42 Glycerin 18 Carrageenan 0.9 Sodium lauryl sulfate 1.2 Saccharin sodium 0.5 Butyl paraoxybenzoate 0.005 Fraction * 1.0 Fragrance 1.0 Water Remaining amount 100 (wt%) * Obtained in Example 4 Fraction

[0030]

Example 13 Mouthwash Ethyl Alcohol 15 Sorbitol 5 Glycerin 7 Sodium Lauryl Sulfate 0.8 1-Menthol 0.05 Saccharin Sodium 0.1 Alkaline Decomposed Substance * 0.1 Perfume 0.05 Water Remaining Amount Total 100 (% by weight) * Alkali obtained in Example 5 Decomposition The plaque formation inhibitor of the present invention processed and prepared by the above-mentioned composition has an effective plaque formation inhibitory action, has high safety, and shows good taste.

[0031]

According to the present invention, excellent plaque formation containing a plaque formation inhibitory substance which is rich in safety and food processing suitability, has good taste, and can be efficiently extracted from inexpensive food materials. An inhibitor can be obtained.

────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ identification code FI A61P 1/02 A61P 1/02 (58) Investigated field (Int.Cl. ⁷ , DB name) A61K 35/78 A23G 1/00 A23G 3/00 101 A23G 3/30 A61K 7/26 A61P 1/02 BIOSIS (DIALOG) CA (STN)

Claims (5)

(57) [Claims] 1. A plaque formation inhibitor characterized by containing cocoa mass treated with an alkali agent or an extract extracted from a cocoa powder obtained by squeezing oil from the cocoa mass with a polar solvent or a decomposed product thereof. Agent. 2. The plaque formation inhibitor according to claim 1, wherein the alkali agent is a carbonate, bicarbonate or hydroxide of sodium, potassium, magnesium, ammonium or the like. 3. The plaque formation inhibitor according to claim 1, wherein the polar solvent is water or hydroalcohol. 4. The degraded product is a degraded product obtained by reacting an extract with an acid, an alkali or an enzyme, or a fractionated product obtained by fractionating the extract by a usual operation. The plaque formation inhibitor according to claim 1. 5. The plaque formation inhibitor according to claim 1, wherein the plaque formation inhibitor comprising an extract or a decomposition product thereof is a food or an oral product.