JP3112102U - Optical instruments for observing blood vessels, blood flow, and cellular tissues - Google Patents

Abstract

An object of the present invention is to provide an observation image of an observation part while making it possible to easily observe the sample by separating the observation sample from an optical device without giving any physical stimulation to blood vessels, blood flow, and cell tissues in the cells of a living body to be observed. Observation on a monitor TV or, if necessary, observation with an optical instrument with the naked eye.
A transparent gel-like solution is applied to the surface of a human finger (portion close to the boundary line between a human fingernail and skin) to set a visual field to be observed, and around an objective lens. The light 14 is irradiated. At that time, the clear image 13 · A captured by the zoom microscope 18 is optically converted into the enlarged image 13 · B, and further converted into an electrical signal by the CCD camera 25 to be converted into a CCD camera cable. 27. The enlarged image 13 · B is taken into the personal computer 30 through the CCD camera control interface board 29, and the enlarged image 13 · B is reproduced on the monitor TV 32 which displays the observation video signal.
[Selection] Figure 1

Description

  The present invention relates to an observation optical apparatus for efficiently observing blood vessels, bloodstreams, and cell tissues in various living cells in a natural state without external stimulation.

  Conventional observation of blood vessels, blood flow, and tissue in the cells of a living body can be done by physically collecting the cell tissue from the body or peeling or damaging the skin to observe the blood vessels or blood flow. I was magnifying with an optical instrument. In addition, the observation contents disclosed in Japanese Patent Laid-Open Nos. 11-299761 and 2000-206118 as conventional observation optical instruments require high-intensity illumination. Since the device cannot be integrated in a compact manner, and light is simply emitted from the side of the living body, the reflected light emitted from the surface of the living body becomes noise, making it difficult to clearly observe internal blood vessels, blood flow, and cellular tissues. Met.

  With the conventional methods described above, metamorphic changes cannot be made due to external stimulation on blood vessels, blood flow, and cellular tissues in living cells, and it is not possible to observe as it is. Observation and analysis results could not be obtained because it was difficult to observe internal cellular tissues, blood vessels, and blood flow due to reflection at the surface.

  Furthermore, since light does not easily enter the inside of the observation sample, high-luminance light is required, and blood vessels, blood flow, and cellular tissues in the cells of the living body are stimulated by the heat of the high-luminance light. Therefore, it was not possible to observe well in the natural state.

  The present invention is intended to solve the problems of such a conventional configuration, and the purpose of the present invention is to provide blood vessels, blood flows, and cellular tissues in the cells of a living body to be observed. It is an object of the present invention to provide an observation optical instrument that enables efficient observation and accurate analysis in a natural state so as not to cause metaphysical changes due to non-contact and external stimuli.

  In order to achieve the above object, an observation optical apparatus according to the present invention is configured as follows.

  In order to solve the above-mentioned problems, the device according to claim 1 and claim 2 is an optical device for observing a blood vessel, blood flow, and cellular tissue in a cell of a living body to be observed (the observation sample and objective lens Set a distance (for example, stereo microscope, biological microscope, etc.), focus on the surface of the observation sample, and irradiate the observation sample with light from the outside of the objective lens or from the inside of the objective lens. By applying a gel solution of a transparent body or a colored transparent body to the surface of the sample, the reflected light of the surface that could not be solved until now is canceled, and further, the gel state of the transparent body or the colored transparent body The solution efficiently guides the light to the inside of a living body cell even with a small amount of light, and also provides a lens effect by the gel-like solution of the transparent or colored transparent body Distinct vessel is within cells of said organism high resolution Te, blood flow, tissue images emerge come into view. In addition, since the objective lens of the optical instrument can be separated from the observation sample for non-contact observation, the operation is easy to handle, and the observation sample can be observed in a natural state without physical stimulation. The above-mentioned problems are solved by a new observation optical instrument that has never been observed so that accurate metamorphic changes can be observed.

  As described above, according to the device described in claim 1, the field of view to be observed by applying a transparent or colored transparent gel-like solution to the surface of the observation sample is set by an optical instrument, When the visual field to be observed is irradiated with light from around the objective lens, the reflection of the surface of the observation sample is canceled by the transparent or colored transparent gel-like solution, and even inside the cell efficiently even with a small amount of light. The blood, blood flow, and cell tissue images in the cells of the living body with high resolution are clearly obtained by guiding the light and further causing a lens effect by the gel solution of the transparent body or the colored transparent body. There is no physical stimulus, and observation in a natural state becomes possible.

  As described above, according to the invention described in claim 2, the operation can be performed by allowing non-contact observation by separating the objective lens of the optical device from the blood vessel, blood flow, and cellular tissue of the living body to be observed. It is easy to handle, there is no physical stimulation to the blood vessels, blood flow, and cellular tissues of the living body that you want to observe, and it is possible to observe in the natural state, and it is possible to observe metamorphic changes accurately. The blood vessel, blood flow, and tissue images in the cells of the high-resolution living body are optically converted into enlarged images, and the enlarged images are converted into electrical color video signals with a CCD camera. Captured into a personal computer through the connected CCD camera control interface board and reproduced vividly on a monitor TV that displays the electrical color video signal. Providing new observation optics is possible with the effect.

  Hereinafter, an embodiment of an optical device for observing blood vessels, blood flows, and cell tissues in cells of a living body according to the present invention will be described in detail with reference to FIG.

  FIG. 1 is an explanatory diagram schematically showing an outline of observation of blood vessels, blood flow, and cellular tissues in cells of a human fingertip with an observation optical apparatus (zoom microscope) to which an embodiment of the present invention is applied. .

  That is, in the configuration of the embodiment apparatus shown in FIG. 1, the configuration of the optical device for observing blood vessels, blood flow, and cellular tissues in living cells according to the present invention is as follows. A portion close to the boundary line) is coated with the transparent gel-like solution 11 on the surface of 12 to set a visual field to be observed. A light source 23 and an optical fiber 15 irradiate light 14 from around the objective lens 16 onto the visual field to be observed.

  The effect of the transparent gel-like solution 11 cancels the surface reflection of the visual field to be observed, and the transparent gel-like solution 11 guides the light 14 to the inside of the cell, and further brings a lens effect. A clear image 13A is captured by the zoom microscope 18.

  The clear image 13 · A captured by the zoom microscope 18 is optically converted into the enlarged image 13 · B by the objective lens 16 and the zoom lens 19. The enlarged image 13 · B is captured by a CCD camera 25 and converted into an electrical color video signal 13 · C, and the electrical color is supplied to a personal computer 30 through a connected CCD camera cable 27 and a CCD camera control interface board 29. The video signal 13 · C is captured and reproduced from the personal computer 30 on the monitor TV 32 that displays the electrical color video signal 13 · C.

  Here, the transparent gel-like solution 11 may be colored, but preferably has as few bubbles as possible. Further, in observing blood vessels, blood flow, and cellular tissues in living cells, the transparent gel solution 11 is not only transparent but also colored in red, blue, green, etc. according to the contents and purpose of the sample. A transparent gel-like solution or a gel-like solution to which a component is added, for example, a gel-like solution to which a luminescent component is added may be used.

  Here, the light source 23 includes a switching power source 21 that changes the AC power source to DC, a lamp (for example, a halogen lamp) 24, a heat ray cut filter 22 that cuts heat rays, and the light 14 output from the lamp 24 of the transparent body. The optical fiber 15 is guided to the gel solution 11 and irradiated. Since the transparent gel-like solution 11 does not require high-intensity light, LED light sources suitable for the content and purpose of the sample, such as white, red, green, blue, infrared, and ultraviolet LEDs, may be used.

  Here, the zoom microscope 18 includes the optical fiber 15 that guides the light 14 from the light source 23 to the gel solution 11 of the transparent body, and the objective lens 16 that magnifies and captures the clear image 13A. Further, the zoom lens 19 capable of freely enlarging / reducing the image and the lens barrel 20 for guiding the enlarged image 13 / B to the CCD camera 25 are constituted.

  Further, the optically magnified image 13 · B is converted into the electrical color video signal 13 · C by a CCD element (Charge Coupled Device) 26 inside the CCD camera 25, and the CCD camera cable 27 In this configuration, the electric color video signal 13 · C is taken into the personal computer 30 through the connected CCD camera control interface board 29 and reproduced on the monitor TV 32.

  Here, the electrical color video signal 13 · C taken into the personal computer 30 is further converted into, for example, quantification of cell metamorphosis, blood flow velocity measurement by various image measurement / image processing software 31. The electrical color video signal 13 · C, such as quantification of metastasis of blood vessels, is digitized and can be expressed as various evaluations, examinations and diagnoses.

Therefore, in the specific configuration of the apparatus of the present embodiment, the following effects can be obtained as compared with the case of the conventional example described above.
That is, (A) Unlike conventional observation methods, no physical stimulus is given, and observation can be efficiently performed in a natural state.
(B) “Anti-reflective effect on the surface” just by applying a transparent gel-like solution to the surface of the sample to be observed. It has become possible to observe easily with a variety of effects, such as “Lens effect can be captured to capture clear high-resolution images”.
(C) By providing a distance between the sample and the objective lens that captures an image with this observation optical instrument, it is possible to perform simple observation and simple chemical stimulation (for example, confirmation of the effect of drug administration) on the sample. It was.
(D) Furthermore, this enlarged image is captured by a CCD camera, converted into an electrical color video signal, and taken into a personal computer, enabling digitalization of the image and digitization of the image. Observation, diagnosis, and inspection from various directions Became possible.
Etc.

  In the above-described embodiment configuration, the case of observing blood vessels, blood flow, and cellular tissue in living cells and observing an enlarged color image of the observed image is described. However, it is not necessarily used for observation. The gel-like solution is not limited to transparent sugar water, and is preferably a gel solution that is as clear as possible without bubbles.

  In addition, it is not limited to the point where the image is observed by colorization and digitization, and if necessary, the observation from the eyepiece by the microscope with the naked eye, the image observation by the monochrome or the image observation by the analog camera may be performed. Is also possible.

  As described above in detail with reference to the examples, according to the present invention, a visual field to be observed is set by applying a transparent gel-like solution to the surface of blood vessels, bloodstreams, and cell tissues in the cells of a living body to be observed. Then, light is emitted from around the lens. At that time, the reflection on the surface is canceled by the gel solution, and light is further guided to the inside of the cell, and further, a lens effect is brought about on the image, and a clear image can be observed.

  A clear image captured by a zoom microscope for further magnification observation is optically converted into a magnified image. The magnified image is converted into an electrical color video signal by a CCD camera, captured on a personal computer through a CCD camera control interface base connected to a CCD camera cable, and clearly displayed on a monitor TV that displays the electrical color video signal. It becomes possible to provide a new observation optical instrument that can be reproduced and can be used for diagnosis and inspection such as confirmation of metamorphosis by clinical experiments, early detection of many diseases, and the like.

  In addition, digital images can be digitized by taking the electrical color video signals into a personal computer. Furthermore, image digitization can be simplified with image measurement and image processing software, and observation, diagnosis, and inspection can be performed from various directions. The advantage and future leap can be made.

  Configuration explanatory diagram (observation configuration diagram and schematic diagram) schematically showing an overview of a method of observing blood vessels, blood flows, and cell tissues in cells of a human fingertip with an optical apparatus (zoom microscope) to which an embodiment of the present invention is applied. It is an observation sample enlarged view).

Explanation of symbols

11 Transparent gel solution (clear sugar water)
12 fingers 13-A clear image 13-B enlarged image 13-C electrical color video signal 14 light 15 optical fiber 16 objective lens 17 objective lens replacement ring 18 zoom microscope 19 zoom mechanism 20 lens barrel 21 switching power source ( Conversion from AC power to DC power)
22 Heat ray cut filter 23 Light source 24 Lamp (halogen lamp)
25 CCD camera (digital type)
26 CCD device (Charge Coupled Device)
27 CCD camera cable 28 CCD camera cable connector 29 CCD camera control unit 30 Personal computer 31 Image measurement and image processing software 32 TV monitor

Claims (2)

  By applying a transparent or colored transparent gel-like solution to the surface of the biological sample to be observed in order to observe in a natural state without any external stimulus to the biological sample to be observed, An observation optical instrument characterized by being able to observe the blood vessel, blood flow, and cellular tissue state in a cell on the inner side more efficiently than the surface of the biological sample to be observed.   There is no external stimulus to the biological sample to be observed, and in order to efficiently observe and improve the operability in a natural state, the biological sample to be observed is separated from the objective lens of the optical device without contact. The observation optical apparatus according to claim 1, wherein the observation optical instrument can be observed with a lens.

Images