JP3111287B2 - Nutrients with excellent absorption of nitrogen and calcium - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a nutrient having a high nutritional effect containing calcium caseinate hydrolyzate obtained by treating calcium caseinate with a protease of Aspergillus genus. More specifically, a molecular weight of 1000 to 500 having an excellent amino acid absorption promoting effect and calcium absorption promoting effect.
The present invention relates to a nutrient excellent in a nitrogen source and calcium absorption, characterized by containing a calcium caseinate hydrolyzate containing a calcium caseinate peptide as a main component.

[0002]

2. Description of the Related Art Casein, a major protein of milk, has a good amino acid balance, is an excellent nitrogen source for nutrient supply, and is often used for nutritional supplementation. It is also an excellent source of calcium.

[0003] However, in the case of poor absorption of the nitrogen source, in the case of promptly improving nutritional status, and in the case where it is necessary to digest and absorb proteins without imposing a burden on the gastrointestinal tract, casein, which is a protein, is used as it is. Use can be inconvenient.

[0004] Therefore, in order to improve the absorption efficiency of a nitrogen source for supplying nutrients, various proteins other than casein, protein degradation products, and a mixture of free amino acids have been used. Recently, peptides have begun to be used for the purpose of improving nutrition, because peptides absorb faster than a mixture of free amino acids and have no antagonism upon absorption. However, when the absorption efficiency of a peptide or a mixture of free amino acids developed for the purpose of improving the absorption efficiency of the nitrogen source is improved as compared with the case of the protein, when focusing on the absorbed amino acid, A nitrogen source that efficiently and quickly absorbs all essential amino acids has not yet been developed.

[0005] Casein phosphopeptide, which is a trypsin hydrolyzate of casein, is considered to promote the absorption of calcium by the action of solubilizing inorganic calcium salts. Therefore, when casein cannot be digested, or when casein is digested and no inorganic calcium salt is present together with casein, there is a problem that calcium is difficult to be absorbed into a living body.

[0006] Further, a nitrogen source that promotes the efficient absorption of calcium simultaneously with the absorption of amino acids has not yet been developed. Therefore, a conventional nutrient containing a nitrogen source cannot be expected to have an effect of efficiently promoting the absorption of calcium simultaneously with the absorption of amino acids.

[Problems to be solved by the invention]

[0007] The present invention relates to a nutrient containing a peptide which is higher in absorption rate and longer lasting than a mixture of free amino acids as a nitrogen source, and is particularly effective in absorbing all essential amino acids and also promoting calcium absorption. It provides a highly effective nutrient.

[0008]

Means for Solving the Problems The present inventors have conducted intensive studies for the purpose of developing a nutritional supplement containing a peptide which promotes the absorption of amino acids as a nitrogen source and also promotes the absorption of calcium. Molecular weight 1 obtained by decomposing casein calcium with a protease of Aspergillus genus
The present inventors have found that a calcium casein hydrolyzate containing 000 to 5000 casein calcium peptides as a main component achieves the above object, and completed the present invention.

The calcium caseinate hydrolyzate used in the present invention is prepared by suspending and dispersing calcium caseinate as a raw material in water in the range of 2 to 40% by weight, adding a proteinase derived from Aspergillus genus to a temperature of 25 to 70 ° C. The reaction is carried out for 5 to 48 hours while stirring at a temperature in the range described above.

The proteolytic enzyme used in the present invention is an enzyme derived from a genus Aspergillus, and among them, a neutral protease is preferable. Although an enzyme other than the neutral protease can be used, pH adjustment is required at the time of the enzyme treatment, and a salt is generated when the enzyme is neutralized after the treatment, so that the salt must be removed. Commercially available proteolytic enzymes of the genus Aspergillus include, for example, "Amano P" and "Amano A" (manufactured by Amano Pharmaceutical Co., Ltd.), "Morcin"
(Fujisawa Pharmaceutical Co., Ltd.) "Orientase ON"
(Made by Ueda Chemical Industry Co., Ltd.). These enzymes may be used alone or in combination of two or more.

The protease used in the present invention is used in an amount of 0.1 to 10% by weight based on calcium caseinate. When the content of the protease is 0.1% by weight or less, calcium caseinate is hardly decomposed, and the yield of the obtained peptide is low. When the amount of the protease is 10% by weight or more, the decomposition reaction of calcium caseinate occurs without any problem, but there is a problem that it becomes economically expensive.

The treatment temperature with the protease of the present invention is as follows:
A temperature range of 25 to 70 ° C. is desirable. At a treatment temperature of 25 ° C. or less, there is a problem that the activity of the enzyme is low and a long time is required for the decomposition of calcium caseinate. The processing temperature is 70 ° C.
Above, the inactivation of the enzyme is so severe that it is difficult to control the decomposition reaction.

The calcium caseinate hydrolyzate used in the present invention can be obtained by suspending and dispersing calcium caseinate in water in the range of 2 to 40% by weight and decomposing it with an enzyme. 2% by weight
If it is less than 1, the enzymatic decomposition reaction can be carried out without any problem, but the yield obtained is low due to the low concentration. On the other hand, when the content exceeds 40% by weight, the viscosity of the solution increases, and the stirring becomes difficult.
There is a problem that dispersion is difficult to be performed uniformly.

The aqueous solution of calcium caseinate hydrolyzate obtained by the method of the present invention can be used in the form of an aqueous solution as it is, or in the form of a dried powder by a method such as spray drying or freeze drying.

The calcium caseinate hydrolyzate obtained by the method of the present invention has a calcium caseinate peptide having a molecular weight of 1,000 to 5,000 as a main component. The calcium casein hydrolyzate has a high absorption rate of a nitrogen source, is persistent, and can efficiently supply all essential amino acids, and at the same time, can supply calcium. A highly effective nutrient can be provided.

In the nutritional supplement of the present invention, in addition to the calcium casein hydrolyzate, other lipids, carbohydrates, and other nutritive essential or desirable components can be appropriately compounded.

As other nitrogen sources of the nutrient of the present invention, commonly used animal and plant proteins such as casein, milk protein, soy protein, wheat protein and the like can be used.

The lipids used in the present invention include vegetable oils such as sunflower oil, palm oil, rice oil, soybean oil, cottonseed oil, corn oil, sunflower oil, olive oil and coconut oil.
Animal fats and oils such as lard, beef tallow, milk fat and the like can be mentioned, and one or a mixture of two or more thereof can be used.

Examples of the saccharide used in the present invention include cellulose, starch, dextrin, saccharides and sugar alcohols, and one or a mixture of two or more thereof can be used. In addition, as other components, vitamins and minerals commonly used for nutritional supplements can be blended.

The form of the nutritional supplement of the present invention can be appropriately selected according to the intended use, such as a drink, an infusion, and the like. Next, the present invention will be described with reference to examples, but the present invention is not limited to the scope of the examples.

[0021]

【Example】

Embodiment 1 FIG. 500 g of casein calcium powder (from New Zealand) having a calcium content of 1.3% was suspended in 5 l of water, and 10 g of a neutral protease of Aspergillus genus (trade name: Amano P manufactured by Amano Pharmaceutical Co., Ltd.) was added.
The reaction was performed at 5 ° C. for 20 hours. Next, at 80 ° C, 30
The mixture was subjected to a heat treatment for 10 minutes to inactivate the enzyme, followed by filtration. The filtrate was spray-dried to obtain 450 g of calcium casein hydrolyzate.

The obtained calcium casein hydrolyzate was subjected to gel filtration chromatography using Waters high performance liquid chromatography (manufactured by Nippon Waters Limited). Super Loose 1 in analytical column
2 (Pharmacia Co., Ltd.) using a phosphate buffer (pH
= 7) was flowed at a flow rate of 0.5 ml / min and detected at 220 nm. As a result of the measurement, the fraction of the obtained calcium casein hydrolyzate having a molecular weight of 1,000 to 5,000 was 75.2%.

Test Example 1 Amino acid absorption test in rat small intestine An amino acid absorption test of the calcium casein hydrolyzate obtained in Example 1 was performed. A sample injection catheter was inserted into the duodenum of a Wistar male rat weighing about 200 g, and a portal vein blood sampling catheter was inserted into the portal vein. After the operation, a solid meal and water were given freely, and the animals were bred for 2 days. On the 3rd day after fasting overnight, 1 ml of the sample solution was administered to the duodenum,
Portal blood was collected every 5, 10, 20, 30, 60 and 120 minutes after administration.

Blood was collected at 0 hours before sample administration. Amino group in plasma of collected blood is converted to TNBS
(Trinitrobenzenesulfonic acid) method, and individual free amino acids were quantified using a Hitachi 8500 automatic amino acid analyzer. The administration samples were divided into the following three groups, and each of the five samples was administered. Group A: the product of the present invention (20% aqueous solution of calcium casein hydrolyzate) Group B: 20% free amino acid mixture (amino acid mixture of the same composition as the product of the present invention) Group C: 20% calcium caseinate aqueous solution Only base quantification was performed.

As a result of quantifying all amino groups in the portal vein plasma,
As shown in Table 1, the amino group concentration in Group A was clearly higher than those in Group B and Group C. (P <0.05). Furthermore,
Similarly, as a result of quantifying the total free amino acid concentration, as shown in Table 2, the total free amino acid concentration in Group A was higher than that in Group B. Table 3 shows the results of quantifying the concentration of all essential amino acids.
As shown, the total essential amino acid concentration of group A was higher than that of group B (P <0.05). Tables 1, 2 and 3 below
Is shown.

[0026]

[Table 1]

[0027]

[Table 2]

[0028]

[Table 3]

From the above, it can be concluded that Group A clearly has superior amino acid absorption from the intestinal tract as compared with Groups B and C, and is also superior in comparison of the amount of essential amino acids absorbed.

Test Example 2 Calcium absorption test by intestinal loop method The effect of the calcium casein hydrolyzate obtained in Example 1 on the absorption of calcium from the intestinal tract was tested. Weight about 2
After fasting 00 g male Wistar rats overnight, a ligated intestinal loop was formed in the upper part of the small intestine under anesthesia. sample,
0.2 ml of a 40 mM phosphate buffer (pH 7.4) and subsequently a sample solution were injected into a 0.2 ml loop using a syringe. After the injection, the small intestine was returned to the intraperitoneal cavity, left under anesthesia for 2 hours, and then the liquid in the loop was taken out. The solution in the loop was washed and collected using a 0.01 N hydrochloric acid solution.

In another rat, the solution in the loop was immediately collected after the sample injection, and this was set to 0 hour. The total amount of calcium remaining in the solution in the loop at 0 and 2 hours after collection was measured using a calcium measurement kit (Wako Pure Chemical Industries, Ltd .; Calcium C-Test Wako) to calculate the calcium absorption rate (%). The administration samples were divided into the following four groups, and the total calcium concentration in the sample was 28.5 mM.
(Calculated as calcium chloride). The number of cases was four each. Group D: 28.5 mM calcium chloride solution Group E: invention product (10% aqueous solution) Group F: invention product (2.58%) + 21.2 mM calcium chloride solution Group G: 10% calcium caseinate solution

From the results shown in Table 4, the calcium absorption rate of group E was significantly higher than that of group D or G containing the same amount of calcium (P <0.05). Also, in the case of group F in which calcium chloride was added to the product of the present invention, the calcium absorption was almost the same as in the case of group E.

[0033]

[Table 4]

As described above, the calcium casein hydrolyzate of the present invention is superior to other materials in calcium absorption and has an effect of increasing the absorption of inorganic calcium.

Embodiment 2 FIG. 35 g of the calcium casein hydrolyzate obtained in Example 1, 25 g of safflower oil and 160 g of dextrin were put in 780 g of water. Further, 4.0 g of a mineral mixture, 1.0 g of a vitamin mixture, and 1 g of a sucrose fatty acid ester were added, and the mixture was pre-emulsified with a homomixer and then emulsified with a homogenizer. The obtained emulsion is heated at 121 ° C.
Nutrition was obtained by sterilizing for 30 minutes.

Embodiment 3 FIG. 20 g of the calcium caseinate hydrolyzate obtained in Example 1, 15 g of sodium caseinate, 150 g of dextrin, 10 g of sucrose, 20 g of soybean oil, and 5 g of medium-chain triglyceride were placed in 780 g of water. Further, 4.0 g of a mineral mixture, 1.0 g of a vitamin mixture, 1 g of a sucrose fatty acid ester, and a small amount of vanilla flavor were added, preliminarily emulsified by a homomixer, and then emulsified by a homogenizer. The obtained emulsifier was sterilized at 121 ° C. for 30 minutes to obtain a nutrient.

[0037]

According to the present invention, the molecular weight of 1000 to 5 obtained by the present invention is obtained.
The nutrient containing calcium caseinate hydrolyzate whose main component is 000 casein calcium peptide has a high absorption rate of the nitrogen source, is long lasting, and can efficiently supply all essential amino acids, and at the same time,
A supply of calcium can be provided. The nutritional supplement of the present invention is excellent in the absorption of amino acids and also promotes the absorption of calcium at the same time, and thus is useful when their digestion and absorption become a problem or when nutrition is improved.

──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Shigehiro Mori 19-48 Yoshino, Matsumoto City Examiner, Kissei Pharmaceutical Industry Co., Ltd. Seiko Tamura (56) References JP-A-2-154644 (JP, A) JP 47-29577 (JP, A) JP-A-2-69419 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 38/00-38/58

Claims (1)

(57) [Claims] A molecular weight of 1,000 to 5 obtained by treating calcium caseinate with a protein enzyme of Aspergillus genus.
Calcium casein hydrolyzate containing 000 casein calcium peptide as a main component.