JP3079752B2 - Rice disease control method - Google Patents
Rice disease control methodInfo
- Publication number
- JP3079752B2 JP3079752B2 JP04054395A JP5439592A JP3079752B2 JP 3079752 B2 JP3079752 B2 JP 3079752B2 JP 04054395 A JP04054395 A JP 04054395A JP 5439592 A JP5439592 A JP 5439592A JP 3079752 B2 JP3079752 B2 JP 3079752B2
- Authority
- JP
- Japan
- Prior art keywords
- rice
- blight
- pathogenic
- fusarium moniliforme
- moniliforme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、イネに対して弱病原性
の微生物を処理することによりイネばか苗病、イネごま
葉枯病及びイネいもち病を防除する方法に関するもので
ある。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for controlling rice blight seedling disease, rice sesame leaf blight and rice blast by treating microorganisms that are slightly pathogenic to rice.
【０００２】[0002]
【従来の技術】フザリウム・モニリフォルメ（Fusarium
moniliforme) は各種植物に病気を起こす糸状菌で、こ
のフザリウム・モニリフォルメは世界的に分布してい
る。日本では、イネばか苗病、イネ株枯病、ダイズ立枯
病、トウモロコシ赤かび病、ワタ立枯病が知られている
が、特にイネばか苗病は重要病害の一つとなっている。
また、コクリオボルス・ミヤベアヌス（Cochliobolus m
iyabeanus ) はイネごま葉枯病を、ピリキュラリア・オ
リゼ（Pyricularia oryzae) は、イネいもち病を起こ
し、イネの重要病害として知られている。これら３病害
は種子伝染することが知られており、種子消毒はイネ栽
培において重要な作業の一つとなっている。過去におい
て水銀剤で種子消毒することによってこれらの病害を防
除したが、水銀剤の使用中止後ベンツイミダゾール系薬
剤が主流となった。しかし、ベンツイミダゾール耐性の
ばか苗病菌の出現により防除効果の低下が見られ、現在
その代替剤としてイミダゾール系のエルゴステロール生
合成阻害剤が使用されている。2. Description of the Related Art Fusarium moniliforme
moniliforme) is a filamentous fungus that causes diseases in various plants, and this Fusarium moniliforme is distributed worldwide. In Japan, rice blight disease, rice strain blight, soybean blight, corn blight and cotton blight are known, and rice blight disease is one of the important diseases.
Also, Cochliobolus miyabeanus (Cochliobolus m
iyabeanus) causes rice sesame leaf blight and Pyricularia oryzae causes rice blast and is known as an important rice disease. These three diseases are known to be transmitted to seeds, and seed disinfection is one of the important tasks in rice cultivation. In the past, these diseases were controlled by disinfecting seeds with mercury agents, but after the use of mercury agents was discontinued, benzimidazoles became mainstream. However, the control effect has been reduced due to the appearance of benzimidazole-resistant blight seedlings, and imidazole-based ergosterol biosynthesis inhibitors are currently used as substitutes.
【０００３】[0003]
【発明が解決しようとする課題】前記殺菌剤によるイネ
ばか苗病の防除は耐性菌の出現によって防除効果が低下
する可能性が高く、その場合新たなる殺菌剤の開発を必
要とする。本発明はこの殺菌剤による防除に代わる、あ
るいは併用する手段として、新しい防除資材を提供する
ものである。The control of rice blight seedling disease by the fungicide is likely to reduce the control effect due to the emergence of resistant bacteria, in which case a new fungicide needs to be developed. The present invention provides a new control material as a means to replace or use in combination with the fungicide.
【０００４】[0004]
【課題を解決するための手段】本発明者らは、イネばか
苗病菌の病原性を検討中、イネに対しては病原性をほと
んど示さないフザリウム・モニリフォルメ（Fusarium m
oniliforme) を見い出した。これに注目し、非病原性フ
ザリウム・オキシスポラム（Fusarium oxysporum) によ
るサツマイモつる割れ病防除のように、弱病原性フザリ
ウム・モニリフォルメ（Fusarium moniliforme) による
イネばか苗病、イネごま葉枯病及びイネいもち病の防除
を試みた結果、高い防除効果を認め、本発明を完成する
に至った。Means for Solving the Problems The present inventors are examining the pathogenicity of the rice blight seedling fungus, and have studied Fusarium moniliforme, which shows little pathogenicity to rice.
oniliforme). Focusing on this, as in the control of sweet potato vines by non-pathogenic Fusarium oxysporum, rice blight seedling, rice scab and rice blast caused by mildly pathogenic Fusarium moniliforme As a result of the attempt to control pests, a high control effect was recognized, and the present invention was completed.
【０００５】すなわち、本発明は、イネに対して弱病原
性フザリウム・モニリフォルメ（Fusarium moniliform
e) をイネ種子に処理することを特徴とするイネ病害の
防除方法である。[0005] That is, the present invention relates to Fusarium moniliform which is weakly pathogenic to rice.
e) is applied to rice seeds to control rice diseases.
【０００６】この弱病原性フザリウム・モニリフォルメ
（Fusarium moniliforme) は以下の性質を有する。 １）培地上での性質 卵形あるいは棍棒状の小型分生胞子を連鎖状及び擬頭状
に短担子梗（隔膜なし）の先端に形成する。ＰＤＡ（ポ
テトデキストロース寒天培地）上での菌糸伸長は速く、
気中菌糸は綿毛状で、コロニーは白、サーモンピンク、
青あるいは紫色を呈する。リチャーズ液体培地中で、ジ
ベレリンあるいはフザリン酸の産生が認められる場合が
多いが、ジベレリンについては、病原菌よりも産生量が
少ない。 ２）イネに対する病原性 イネ種子を弱病原性フザリウム・モニリフォルメ（Fusa
rium moniliforme) の分生胞子懸濁液5.0X106 個／mlに
２４時間浸漬後、浸種し、播種しても全く徒長苗を生じ
ない。また、開花期に分生胞子懸濁液2.0x105 個／mlを
接種したイネ種子を播種しても徒長苗を生じない。This weakly pathogenic Fusarium moniliforme has the following properties: 1) Properties on culture medium Small ovoid or club-shaped conidia are formed at the tip of short basidiomycetes (without diaphragm) in a chain-like or pseudo-head-like manner. Hyphal elongation on PDA (potato dextrose agar) is fast,
Aerial hyphae are fluffy, colonies are white, salmon pink,
Presents blue or purple. Production of gibberellin or fusaric acid is often observed in Richards' liquid medium, but the production of gibberellin is smaller than that of pathogenic bacteria. 2) Pathogenicity to rice Rice seeds are transformed into a weakly pathogenic Fusarium moniliforme (Fusa
rium moniliforme), immersed in 5.0 × 10 6 conidia spore suspension / ml for 24 hours, then immersed and seeded, no seedlings were produced. In addition, seedling of rice seeds inoculated with 2.0 × 10 5 conidia spore suspension / ml during the flowering stage does not produce seedlings.
【０００７】この弱病原性フザリウム・モニリフォルメ
（Fusarium moniliforme) は、ＰＤＡで１０〜１４日
間、２５℃前後で培養した後、多数の胞子を形成する。
この胞子を水に懸濁させ、ばか苗病、イネごま葉枯病あ
るいはイネいもち病に感染したイネ種子を胞子懸濁液に
一定時間浸漬する。この種子を発芽させ、播種すると発
病苗が著しく減少し、優れた防除効果を示す。[0007] This weakly pathogenic Fusarium moniliforme forms a large number of spores after being cultured at around 25 ° C for 10 to 14 days on PDA.
The spores are suspended in water, and rice seeds infected with blight seedling disease, rice sesame leaf blight or rice blast are immersed in the spore suspension for a certain period of time. When the seeds are germinated and sown, the number of diseased seedlings is remarkably reduced, and an excellent control effect is exhibited.
【０００８】[0008]
【作用】非病原性フザリウム・オキシスポラム（Fusari
um oxysporum) によるサツマイモつる割れ病（Fusarium
oxysporum f.sp.batatas)やバーティシリウム萎凋病
（verticillium dahliae) の防除の場合、その作用は植
物の誘導抵抗性であると考えられている。本発明におい
ても弱病原性フザリウム・モニリフォルメにより抵抗性
が誘導されているかどうかは不明であるが、病原菌と競
合し、病原菌の繁殖の場を少なくすることにより発病を
抑制するのではないかと考えられる。[Action] Non-pathogenic Fusarium oxysporum (Fusari
um oxysporum) caused by sweet potato vine scab (Fusarium)
In the control of oxysporum f.sp.batatas) and verticillium dahliae, the action is believed to be plant induced resistance. In the present invention, it is unknown whether resistance is induced by the weakly pathogenic Fusarium moniliforme, but it is thought that it will compete with the pathogenic bacteria and suppress the onset of disease by reducing the place of propagation of the pathogenic bacteria. .
【０００９】[0009]
【実施例】以下、実施例について具体的に説明する。 実施例１ 静岡県榛原郡榛原町の水田で収穫したイネ種子を表面殺
菌し、この種子をＰＣＮＢ 1000 ppm 、ストレプトマイ
シン 300ppm 含有のＰＤＡに置床し、各種微生物を得
た。このうちフザリウム・モニリフォルメ（Fusarium m
oniliforme) を分離し、この中からイネに対して弱病原
性フザリウム・モニリフォルメ（Fusariummoniliforme)
を得た。これらの菌の諸性質を検討し、記述通りの結
果を得た。この弱病原性フザリウム・モニリフォルメ
（Fusarium moniliforme) を培養し、分生胞子を形成さ
せ分生胞子懸濁液を調整した。イネの品種、日本晴の開
花期にばか苗病菌を接種し、収穫した籾を上記で調製し
た分生胞子懸濁液に２４時間浸漬した後、水に浸種し催
芽した。播種後３〜４葉期の頃、徒長苗数を調査し、防
除効果を判定した。EXAMPLES Examples will be specifically described below. Example 1 Rice seeds harvested in a paddy field in Haibara-cho, Haibara-gun, Shizuoka Prefecture were surface-sterilized, and the seeds were placed on a PDA containing 1,000 ppm of PCNB and 300 ppm of streptomycin to obtain various microorganisms. Of these, Fusarium m
oniliforme) from which Fusarium moniliforme is a mildly pathogenic to rice.
I got Various properties of these bacteria were examined, and the results were as described. This weakly pathogenic Fusarium moniliforme was cultured to form conidia and prepare a conidia spore suspension. The rice variety, Nipponbare, was inoculated with blight seedlings during the flowering period, and the harvested paddy was immersed in the conidia suspension prepared above for 24 hours, then immersed in water and germinated. At the 3-4 leaf stage after sowing, the number of seedlings was examined to determine the control effect.
【００１０】[0010]
【数１】 (Equation 1)
【００１１】[0011]
【表１】 [Table 1]
【００１２】結果は第１表に示す通り、弱病原性フザリ
ウム・モニリフォルメ（Fusarium moniliforme) は、イ
ネばか苗病に対して明かな徒長抑制効果を示した。対照
に用いたフザリウム・オキシスポラム（Fusarium oxysp
orum) は全く徒長抑制効果を示さなかった。As shown in Table 1, the weakly pathogenic Fusarium moniliforme showed a clear inhibitory effect on rice stunt seedling disease. Fusarium oxysporum used as a control
orum) did not show any effect on the suppression of the length.
【００１３】実施例２ イネの品種、新潟早稲（実施例１と同様、接種籾）を用
い、実施例１と同じ方法で弱病原性フザリウム・モニリ
フォルメ（Fusarium moniliforme) を処理した。Example 2 A weakly pathogenic Fusarium moniliforme was treated in the same manner as in Example 1 by using a rice cultivar, Niigata Waseda (inoculated paddy as in Example 1).
【００１４】[0014]
【表２】 [Table 2]
【００１５】結果は第２表に示す通り、弱病原性フザリ
ウム・モニリフォルメ（Fusarium moniliforme) の胞子
濃度が 1.0x104個／mlでも高い徒長制御効果が認められ
た。As shown in Table 2, a high control effect on the length of the plant was observed even when the spore concentration of the weakly pathogenic Fusarium moniliforme was 1.0 × 10 4 / ml.
【００１６】実施例３ 実施例１や２と異なり、イネ品種ササニシキの健全種子
を用いた。この健全種子をイネばか苗病菌の胞子懸濁液
に２４時間浸漬した後、浸種し、発芽直前に弱病原性フ
ザリウム・モニリフォルメ（Fusarium moniliforme) の
胞子懸濁液に、２４時間浸漬し、播種した。あるいは、
この逆の場合や、イネばか苗病菌と弱病原性フザリウム
・モニリフォルメ（Fusarium moniliforme) の胞子懸濁
液を混ぜ、健全籾を処理し、実施例１と同様の方法で調
査した。Example 3 Different from Examples 1 and 2, healthy seeds of rice cultivar Sasanishiki were used. The healthy seeds were immersed in a spore suspension of rice blight seedling disease for 24 hours, immersed in the spore suspension, and immediately before germination, immersed in a spore suspension of a mildly pathogenic Fusarium moniliforme for 24 hours and sown. . Or,
In the reverse case, or by mixing a spore suspension of Fusarium moniliforme and a mildly pathogenic Fusarium moniliforme, a healthy paddy was treated and investigated in the same manner as in Example 1.
【００１７】[0017]
【数２】 (Equation 2)
【００１８】[0018]
【表３】 [Table 3]
【００１９】結果は第３表に示す通り、いずれの処理方
法でも弱病原性フザリウム・モニリフォルメ（Fusarium
moniliforme) は、ばか苗病菌による徒長を著しく抑制
し、高い防除効果が認められた。As shown in Table 3, the results were as follows. In all treatment methods, the mildly pathogenic Fusarium moniliforme (Fusarium) was used.
moniliforme) remarkably suppressed the growth caused by the idiot fungus and showed a high control effect.
【００２０】実施例４ 次に、イネごま葉枯病に自然感染したムツホマレを用
い、実施例１と同じ方法で弱病原性フザリウム・モニリ
フォルメ（Fusarium moniliforme) を処理した。播種後
３〜４葉期の頃、発病苗数を調査し、防除効果を判定し
た。Example 4 Next, a weakly pathogenic Fusarium moniliforme was treated in the same manner as in Example 1 using Mutshomare naturally infected with rice sesame leaf blight. At the 3-4 leaf stage after sowing, the number of diseased seedlings was investigated to determine the control effect.
【００２１】[0021]
【数３】 (Equation 3)
【００２２】[0022]
【表４】 [Table 4]
【００２３】結果は第４表に示す通り、弱病原性フザリ
ウム・モニリフォルメ（Fusarium moniliforme) は、イ
ネごま葉枯病に対して明かな発病抑制効果を示した。As shown in Table 4, the weakly pathogenic Fusarium moniliforme showed a clear inhibitory effect on rice sesame leaf blight.
【００２４】実施例５ 次に、イネいもち病に自然感染した日本晴を用い、実施
例１と同じ方法で弱病原性フザリウム・モニリフォルメ
（Fusarium moniliforme) を処理した。播種後３〜４葉
期の頃、発情苗数を調査し、防除効果を判定した。Example 5 Next, Nipponbare naturally infected with rice blast was treated with Fusarium moniliforme in the same manner as in Example 1. At the 3-4 leaf stage after sowing, the number of estrus seedlings was examined to determine the control effect.
【００２５】[0025]
【表５】 [Table 5]
【００２６】結果は第５表に示す通り、弱病原性フザリ
ウム・モニリフォルメ（Fusarium moniliforme) は、イ
ネいもち病に対して明かな発病抑制効果を示した。As shown in Table 5, the weakly pathogenic Fusarium moniliforme showed a clear inhibitory effect on rice blast.
【００２７】[0027]
【発明の効果】本発明に係るイネに対して弱病原性のフ
ザリウム・モニリフォルメ（Fusariummoniliforme)
は、イネに対して病原性も無く、また病害防除方法は、
イネばか病、イネごま葉枯病及びイネいもち病に対して
高い効果を示すことから、農業生産に有用である。According to the present invention, Fusarium moniliforme which is weakly pathogenic to rice according to the present invention.
Is not pathogenic to rice, and the disease control method is
It is highly effective against rice blight, sesame leaf blight and rice blast, and thus is useful for agricultural production.
Claims (1)
ニリフォルメ（Fusarium moniliforme) をイネの種子に
処理することを特徴とするイネ病害の防除方法。1. A method for controlling rice diseases, comprising treating rice seeds with Fusarium moniliforme, which is weakly pathogenic to rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP04054395A JP3079752B2 (en) | 1991-02-05 | 1992-02-05 | Rice disease control method |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3-35230 | 1991-02-05 | ||
| JP3523091 | 1991-02-05 | ||
| JP04054395A JP3079752B2 (en) | 1991-02-05 | 1992-02-05 | Rice disease control method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0565209A JPH0565209A (en) | 1993-03-19 |
| JP3079752B2 true JP3079752B2 (en) | 2000-08-21 |
Family
ID=26374173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP04054395A Expired - Fee Related JP3079752B2 (en) | 1991-02-05 | 1992-02-05 | Rice disease control method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3079752B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU670222B2 (en) * | 1993-12-28 | 1996-07-04 | Japan Tobacco Inc. | Disease control agent for useful gramineous plants and control method |
| JP3085895B2 (en) | 1995-11-20 | 2000-09-11 | 日本たばこ産業株式会社 | Novel strain belonging to Exerohilum monoceras and its use |
| CN110558337A (en) * | 2019-07-19 | 2019-12-13 | 湖南科技学院 | biocontrol preparation for preventing and treating rice blast and preparation method thereof |
-
1992
- 1992-02-05 JP JP04054395A patent/JP3079752B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0565209A (en) | 1993-03-19 |
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