JP3077975B1 - Novel microorganism having fat and oil decomposability, and method of treating wastewater containing fat and oil using the novel microorganism - Google Patents

Abstract

The present invention provides a novel fat-decomposing microorganism. SOLUTION: A novel microorganism S. saprophyticus OD-1 (FER) belonging to the genus Staphylococcus and having a decomposability in fat and oil components.
MP-17201).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a novel microorganism capable of decomposing animal and vegetable oils and fats.

[0002]

2. Description of the Related Art Wastewater often contains a large amount of animal and vegetable oils and fats, and the discharge of such wastewater as it is to public sewers is strictly regulated from the viewpoint of maintaining the environment of water bodies. Also, wastewater standards are set for the purpose of such regulations, and each business entity is required to take measures such as installing oil and fat exclusion equipment.

[0003] However, the installation of a conventionally used grease trap or other grease removal facility complicates problems such as accumulation of solidified grease, blockage of sewer pipes, generation of offensive odors, and exceeding of drainage standard values. .

[0004] One of the wastewater treatment techniques for solving this problem is a biological treatment method. Among them, the activated sludge method is particularly well known. This is a method for removing or decomposing pollutants including oils and fats in wastewater using various organisms existing in nature, particularly microorganisms.

[0005] Several microorganisms which are commercially available and especially used for treating, decomposing and removing fats and oils in wastewater, and microbial preparations thereof are known. The main ones are as follows. That is, bacteria include Bacillus.sp., Psuedomonas.sp., And the like, protozoa include amoeba, ciliates, and flagellates, and algae include cyanobacteria, green algae, and diatoms. .

[0006]

These substances can decompose organic substances including fats and oils and some inorganic substances. However, they can be used for wastewater treatment, their safety, the activity of decomposing fats and oils, and the decomposition of fats and oils. Efficiency, fat and oil decomposition time, etc. were not always satisfactory.

Further, there is a problem to be solved in a treatment apparatus for decomposing and removing fats and oils in wastewater using these microorganisms, and in a method for maximally and stably maintaining the decomposition activity of the microorganisms. .

[0008]

Means for Solving the Problems The present inventors have conducted intensive studies to solve the above-mentioned problems, and have succeeded in discovering and separating novel microorganisms which can decompose oils and fats in water with high efficiency. The invention has been completed.

The present invention provides a novel microorganism, S. saprophyticu, which has a high decomposition activity against animal and vegetable oils and fats.
s OD-1 (FERM P-17201). Also,
The present invention relates to a microorganism preparation comprising the microorganism, and a wastewater treatment method for decomposing and removing fats and oils in wastewater treatment water using the microorganism preparation. Further, the present invention provides the use of the microorganism,
For example, the present invention relates to a method for producing an lipolytic substance, which comprises separating and purifying a novel lipolytic substance directly from the microorganism or from a culture solution thereof. The present invention also relates to a microbial preparation for decomposing fats and oils containing the above-mentioned microorganism. Further, the present invention is a wastewater treatment device for treating wastewater using the microorganisms, comprising a diffuser for blowing air into the wastewater, and a trap for preventing the microorganisms from flowing out. The present invention relates to a characteristic wastewater treatment device. The fats and oils to be decomposed and removed by the microorganisms according to the present invention are mainly derived from animals and plants, but are not necessarily limited to their origin, and were obtained by methods including various synthetic chemical techniques. It also includes artificial fats and oils, derivatives thereof, and other various hydrocarbons.

[0010]

[0011]

Further, the present invention provides a treatment apparatus for decomposing and removing fats and oils in waste water, characterized by using the waste water treatment method according to the present invention.
In particular, a processing apparatus characterized by having an air diffuser for blowing air in order to maintain high decomposition activity, and a trap for preventing the outflow of the microorganisms or the microbial preparation and separating the oil from the oil. To provide.

[0013]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail with reference to embodiments of the present invention.

A. Microorganisms capable of decomposing oils and fats present in an aqueous solution of microorganism screening can be separated from soil. Soil is generally said to be less toxic, and microorganisms isolated from such soil are safe (friendly) for the human body. The search for the useful strain can be performed by using a commonly used animal and vegetable oil and fat component and selecting an appropriate medium and culture conditions. More specifically, a commercially available salad oil can be used as the oil component. Further, as the culture conditions, specifically, the culture can be performed by culturing using a Luria-Bertani medium (hereinafter abbreviated as LB medium). Culture conditions can be easily set by those skilled in the art based on the used medium and the like. For example, the method described in "Microbiological Experiment Method, Microbial Research Method Roundtable, edited by Kodansha (1993)" can be preferably applied.

The microorganisms (bacteria) that decompose the fats and oils obtained by the culture can be separated by various known methods, specifically, by culturing on an LB plate medium containing agar. It is possible.

B. Identification of strains Identification, main properties, properties, and the like of the isolated strains can be performed using various known identification test methods or commercially available identification kits. Is easy for Specifically, "Bergey's Manua
l of DetectiveBacteriology, Holt, JG, etc., William
s & Wilkins (1994) ".

C. Decomposition test of oils and fats in wastewater by microorganisms
Test Decomposition of fats and oils in wastewater by the microorganism of the present invention is possible by measuring the decomposition amount and decomposition rate of various fats and oils components. Further, under the same conditions, comparison with degradation by various known microbial preparations can be easily performed, and the effect of the microorganism of the present invention or the microbial preparation can be tested.

More specifically, a culture medium or a drainage sample is placed in a culture flask having an appropriate volume, sterilized by an autoclave, and then cultured under appropriate conditions by adding an appropriate fat or oil component. After the cultivation, the fats and oils were extracted with n-hexane, and after removing the solvent, the weight of the remaining fats and oils was measured (similarly, a control (no bacteria added) experiment was carried out). Can be performed.

D. Microbial preparations The microorganisms of the present invention can be formulated into various forms by immobilizing them with various known carriers. For example, `` Applied Microbiology Revised Edition, Sawao Murao and Motoo Arai, edited by Baifukan (1993) '', `` Introduction to Biotechnology, Eizo Sada, Takeshi Kobayashi, Hiroyuki Honda, Kodansha Scientific Hook (1
996) "," Industrial enzymes, written by Takayuki Uejima, Maruzen Co., Ltd. (199
By using the method described in "5)," the microorganism according to the present invention can be formulated.

(1) Liquefaction: The powder can be formed by the following steps. (i) the microorganism of the present invention in a nutrient medium for general bacteria such as LB medium;
A preparation is prepared by culturing for about 24 hours or adding a pH adjuster or the like to this. (ii) A preparation is prepared by appropriately concentrating the culture of (i) by freeze-drying or the like, or by adding a pH adjuster thereto. (iii) Cells obtained by recovering cells from the culture of (i) by centrifugation or the like and suspending them in a medium such as physiological saline to an appropriate concentration, or adding a pH adjuster or the like thereto. As a preparation. (iv) The cells are collected from the culture of (i) by centrifugation or the like and suspended in a fresh LB medium, or a suspension prepared by adding a pH adjuster or the like to the preparation. (v) and (iv) are further appropriately concentrated by freeze-drying or the like to prepare a preparation.

(2) Powdering: Powdering can be performed by the following steps. (i) the microorganism of the present invention in a nutrient medium for general bacteria such as LB medium;
A preparation is prepared by culturing for about 24 hours or by adding a pH adjuster or the like to this and then drying it by freeze-drying or the like. (ii) A formulation prepared by appropriately adding fine powder such as fiber waste and sawdust to (i). (iii) The cells were collected from the culture of (i) by centrifugation or the like, suspended in a medium such as physiological saline so as to have an appropriate concentration, or a pH adjuster was added thereto, and lyophilized. The product dried by the method is used as a preparation. (iv) A formulation prepared by appropriately adding fine powder such as fiber waste and sawdust to (iii). (v) Collect the cells from the culture of (i) by centrifugation, etc.
A formulation is prepared by suspending in an LB medium or by adding a pH adjuster or the like to the suspension and freeze-drying or the like. (vi) A formulation is prepared by appropriately adding fine powder such as fiber waste and sawdust to (v). (vii) Further, the microorganism of the present invention can be granulated by a method described in a known document, for example, “Industrial enzyme, Takayuki Uejima, Maruzen Co., Ltd. (1995)”.
Specifically, the cell suspension prepared in the same manner as in the above (i) to (v) can be used as prim granules, malm granules, film-coated malm granules, T granules containing cellulose fibers, and the like. is there.

(3) Immobilization: Various techniques for immobilization can be used for immobilization by known techniques such as a carrier binding method, a cross-linking method, an entrapment method, and a composite method.

E. Wastewater treatment method and treatment apparatus There is no particular limitation on the treatment method for wastewater containing oils and fats using the microorganisms and microbial preparations according to the present invention. The wastewater treatment method, and further, the treatment apparatus and the treatment equipment can be used without major changes. At this time, it is easy for those skilled in the art to optimize the processing conditions, the operating conditions of the processing apparatus, and the like in accordance with the properties of the microorganism according to the present invention.

Further, the method for treating wastewater containing oils and fats using the microorganisms and microbial preparations of the present invention, and the treatment apparatus therefor, generate odors due to putrefaction, decrease the ability to decompose oils and fats due to putrefactive bacteria, sludge and scum. It is preferable to remove solid matter such as garbage which causes generation by a screen or the like. In addition, blowing air by electric pump or pneumatic drive to maintain high decomposition activity,
It is preferable to install a diffuser for that purpose. Further, it is preferable to have a trap for preventing the outflow of the microorganisms or the microbial preparation and for bringing the microorganisms or the microbial preparation into sufficient contact with oil. More specifically, as the trap for contacting the oil, a membrane filter having a predetermined pore size (not allowing microorganisms to permeate, but allowing only water and low molecular compounds to pass) can be used. In the case of wastewater containing a large amount of animal fats and oils that solidifies at a low temperature, a higher effect can be obtained by controlling the temperature of the wastewater in the trap using a heater or the like.

F. The lipolytic substance according to the present invention is a component or a composition secreted by the lipolytic microorganism according to the present invention described above, and may be an animal, vegetable or synthetic oil or a derivative thereof. Can be decomposed.

The lipolytic substance is contained in a culture solution obtained when the lipolytic microorganism according to the present invention is cultured under appropriate culture conditions. This culture solution can be used directly for decomposing oils and fats in wastewater, but if necessary, it can be separated using a known method.

The method for testing the fat and oil decomposability of the obtained fat and oil decomposable substance can be performed in the same manner as that of the fat and oil decomposable microorganism.

Further, the method of using the obtained fat-decomposable substance for decomposing and removing fats and oils in wastewater is not particularly limited.
It is also possible to add the compound itself to wastewater, or to immobilize it on a carrier or the like to form a formulation and use it.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to these examples.

[0030]

EXAMPLE Acquisition of the microorganism according to the present invention The microorganism according to the present invention was obtained by searching from soil. Search contains 10% commercial salad oil
The enrichment culture was performed using a Luria-Bertani medium (hereinafter abbreviated as LB medium, pH 7.2). Enrichment culture is φ
The test was performed by placing 5 ml of LB medium in a test tube of 18 mm x 180 mm and immersing and culturing (170 strokes / min) at 37 ° C.

Separation of fat-decomposing bacteria was carried out using LB containing 1.5% agar.
This was performed by culturing at 37 ° C. on a plate medium. The identification of the isolated strain was performed using a commercial identification kit ID32STAPH (bioMerieux sa, Mar.
cy l'Etoile, France) and obtained the following results.

The test methods for the properties of the isolated strains were performed according to the methods summarized below. Shape and size: Observe with an optical microscope at a magnification of 400 to 1,000 times. Test strain is 37 ℃ on LB or normal agar plate medium
X 1 day, pick from colonies that occur. The normal agar medium used was 5 g of meat extract, 10 g of peptone, 5 g of NaCl,
It consists of 15g of agar and 1,000ml of distilled water.

Gram staining: Modified by Hucker's method. Positives are determined by crystal violet purple and negatives by counterstained safranin red. The test bacterium is cultured at 37 ° C. for 1 day on a normal agar plate medium, and is collected from the generated colony.

Spores: The presence or absence of spores is determined by 1) heating a culture solution shake-cultured in a normal broth medium at 85 ° C. for 15 minutes, diluting and culturing on a normal agar plate medium, and determining the occurrence of colonies. . The normal broth medium used consisted of 3 g of meat extract, 5 g of peptone and 1,000 ml of distilled water. Or 2) Wirtz's law
Spores are stained by (Schaeffer-Fulton's modification), and the bacterial cells are judged to be red and the spores are judged to be green. 0.5% red color
Safranine aqueous solution, green color of spores is due to 5% malachite green aqueous solution.

Motility: 1) Flagella are stained by the method of Nishizawa and Sugawara, and observed with an electron microscope (magnification: 10,000).
The liquid is composed of 100 ml of tannic acid, 1.5 ml of a ferric chloride solution in the form of pharmacy, 2 ml of formalin, 1 ml of 1% NaOH, and 100 ml of distilled water, and the second liquid is composed of 2 g of silver nitrate, a small amount of the aqueous ammonia solution, and 100 ml of distilled water. Alternatively, 2) puncture culture was performed on a semi-fluid ordinary agar high-layer medium, and the turbidity of the entire medium was determined. The semi-fluid agar medium used is meat extract 5g, peptone 10g, NaCl 5g, agar 5
g, consisting of 1,000 ml of distilled water.

Acid resistance: Acid resistance is determined by the Ziiehl-Neeisen staining method. Positive is determined by red of fuchsin carbonate and negative is determined by green of counterstained malachite green. The test bacterium is cultured at 37 ° C. for 1 day on a normal agar plate medium, and is collected from the generated colony. Attitude toward oxygen: [Medium composition] (1,000 ml of distilled water)
Medium, commercially available normal agar medium) meat extract 5g, sodium chloride 5
g, peptone 10 g, agar 15 g (pH 7.0 ± 0.1). [Culture conditions] In a sterilized test tube, the bacteria and the medium are mixed and fixed in a high layer and cultured. 37 ° C x 1 day.

[Observation items] The presence or absence of aerobic or anaerobic growth. Oxidase: [Medium composition] (in 1,000 ml of distilled water) 5 g of meat extract, 10 g of peptone, 5 g of sodium chloride, 15 g of agar
[Culture conditions] A medium is fixed on a sterile petri dish, and streaked culture is performed thereon. 37 ° C x 1 day. [Observation items] Presence / absence of cytochrome: Dimetyl-p-phenylened
A 1% aqueous solution of iamine was dropped, and the color was determined to be positive when the drop color changed to black via pink.

Catalase: [Medium composition] (distilled water 1,00
0ml) meat extract 5g, peptone 10g, sodium chloride 5g,
Agar 15g. Culture conditions] Fix the medium on a sterile petri dish,
Streak culture is performed thereon. 37 ° C x 1 day. [Observation items] The presence or absence of catalase: the presence or absence of an enzyme that catalyzes the decomposition of hydrogen peroxide. One drop of 3% H ₂ O ₂ solution is placed on a slide glass, and one platinum loop of the bacterium is mixed well with the drop. When oxygen bubbles were generated in a large amount or continuously, it was determined to be positive.

Lipase: [Medium composition] LB plate medium containing 1% commercial salad oil. [Culture conditions] A medium is fixed on a sterile petri dish, and streaked culture is performed thereon. 37 ° C x several days. [Observation items] Presence or absence of lipase: Presence or absence of an enzyme that hydrolyzes an ester bond of a fatty acid ester or lipid. Positive was determined when a phenomenon (hereinafter, halo) was observed around the bacteria grown on the medium. [TLC analysis of decomposed fats] In order to clarify the process of decomposition of decomposed fats and oils by vegetable oil decomposition by lipolytic bacteria, analysis was performed by TLC (thin layer chromatography) by the following method. . As a developing solvent, petroleum ether: diethyl ether: acetic acid (7: 3: 2, volume ratio) was used. As a coloring reagent, a 0.5% sulfuric acid-methanol solution was used. The remaining oil extracted after each culture time was diluted 10-fold with n-hexane as a sample, and spotted 1.5 cm from the lower end of a thin layer plate (Diamond K6FSilica gel 60 Å, Whatman) (spot). Was made to be about 2 mm in size and spotted repeatedly twice). Developing solvent (about
20 ml) was placed in a 500 ml beaker and the plate on which the sample was spotted was placed). The developing tank was sealed with a plastic film. After the developing solvent was raised to about 1 cm from the upper end of the plate, the plate was taken out. After drying,
The coloring reagent was sprayed on the plate surface, dried, and heated on a hot plate to develop a color. The production of fatty acids was confirmed from the Rf value of each spot.

Protease: [Medium composition] (distilled water 1,
000ml) skim milk 30g, meat extract 5g, peptone 10
g, sodium chloride 5g, agar 15g. [Culture conditions] A medium is fixed on a sterile petri dish, and streaked culture is performed thereon. 37 ℃ ×
Several days. [Observation item] Presence or absence of protease: Presence or absence of an enzyme that hydrolyzes protein. When a halo was observed, it was determined to be positive.

Amylase: [Medium composition] (1,00 distilled water)
In 0ml) soluble starch 10g, meat extract 5g, peptone 10
g, sodium chloride 5g, agar 15g. [Culture conditions] A medium is fixed on a sterile petri dish, and streaked culture is performed thereon. 37 ℃ ×
Several days. [Observation items] Presence or absence of amylase: Presence or absence of an enzyme that hydrolyzes starch. Lugol's solution was applied around the grown bacteria, and it was determined to be positive when it did not show blue-violet coloring.

Biosurfactant-producing ability: [Medium composition] Commercially available blood agar medium (manufactured by Eiken Chemical Co., Ltd.). [Culture conditions] A medium is fixed on a sterile petri dish, and streaked culture is performed thereon. 37 ° C x several days. [Observation items] Biosurfactant production ability: presence or absence of a substance that emulsifies fats and oils. When a halo was observed, it was determined to be positive.

Production of acid from glucose under anaerobic condition (O
-F test): [Medium composition] (in 1,000 ml of distilled water) glucose 10 g, peptone 2.7 g, sodium chloride 10 g, potassium monohydrogen phosphate 0.3 g, bromothymol blue 0.06 g,
4g agar. [Culture conditions] The above soft agar high-layer medium is fixed in a test tube, and after puncturing the bacteria, liquid paraffin is overlaid on one side and cultured. 30 ° C x several days. [Observation item] Acid generation ability: presence or absence of glycolysis. When both yellowed, it was judged as fermentation type.

Dye: [Medium composition] (1,000 ml of distilled water)
Middle) peptone 20g, glycerin 10g, K_TwoSO_Four 10g, MgCl_Two1.
4g agar 15g. [Culture conditions] Fix the medium on a sterile petri dish
And streak culture on it. 25 ° C x 5 days. [Observation items]
Pigment formation: water-insoluble when colored only around colonies
Water-soluble pigments are formed when the entire medium is colored
It was determined.

Further, the properties shown in Table 2 were determined according to the manual attached to a commercially available kit (manufactured by BioMerieux Biotech).

The properties of this bacterium are summarized in Tables 1 and 2 below.
As a result, this bacterium was identified as Staphylococcus saprophyticus. The bacterium was named S. saprophyticus OD-1 and was registered with the Patented Microorganisms Depositary Center of the Institute of Biotechnology and Industrial Technology of the Ministry of International Trade and Industry on February 5, 1999, at FERM.
Deposited as P-17201.

The bacterium can be stored at 4 ° C. for at least 30 weeks, and has a risk level of 1, which is very safe and very convenient to handle.

(Table 1 Main Properties of Isolated Strains) ====================================== ======== Item ----------------------------------------- ---- Form Staphylococcus aureus Size 0.65 μm Gram stain + Spores-Motility + Attitude to oxygen Aerobic oxidase + Catalase + Lipase + Protease-Amylase-Biosurfactant-producing ability + Production of acid from glucose under anaerobic condition − Settlement color NP ============================================== = NP: Does not produce characteristic colony pigment

(Table 2 Properties of Isolated Bacteria) ============================================== ======= Items ------------------------------------------ ---- Urease + arginine dihydrolase-ornithine decarboxylase-hydrolysis of esculin-acid formation glucose + fructose + D-mannose-D-maltose + lactose + trehalose + D-mannitol + raffinose-sucrose + N- Acetylglucosamine + D-turanose + L-arabinose-D-ribose-D-cellobiose-Reduction of nitrate-Formation of acetoin-β-galactosidase + β-glucuronidase-Arginine allyl amidase-Alkaline phosphatase-Pyrrolidonyl allyl amidase + Novobiocin resistance + ================================================

5 ml was aseptically collected from the growth medium of the bacteria, centrifuged at 10,000 × g for 5 minutes, and the cells were suspended in distilled water. This was performed three times to wash the cells. The washed cells were dried at 105 ° C. to a constant weight, and then weighed. This was defined as the dry weight of the cells.

[0051] measurement of total oxygen consumption total oxygen consumption, was carried out using a Yuasa Ionics Inc. total oxygen consumption spectrometer TOD1248NS.

Measurement of Degradation of Vegetable Oil (1) Preparation of Microbial Suspension: This test uses S. saprophyticus
Using OD-1 (FERMP-17201), a microorganism suspension was prepared as follows. LB medium (pH
7.2) One platinum loop was inoculated with 5 ml of the preserved bacteria, and shaking culture was performed overnight at 28 ° C. This was used for the following experiments. In order to compare the fat and oil decomposition rates, five commercially available microbial preparations from two companies (A and B) and three (3) companies from Japan (A, B and C)
Four formulations from the company were used. The fat-decomposing microorganisms contained in each are Bacillus sp. Or a mixed bacterium (Bacillus sp.
p.) or a mixed microorganism group.

LB medium containing 1% of commercially available salad oil
(pH 7.2), and cultured with shaking at 28 ° C. for 24 hours as described above. This preculture solution was subjected to the following fat / oil decomposition test. (2) Wastewater sample: Wastewater from two restaurants and one food factory was collected and used as it was in the experiment. (3) Degradation reaction and determination of effect: 100 ml of LB medium or other medium (Table 3) or drainage sample (Table 4) was placed in a 500-ml baffled culture flask, and sterilized by autoclaving at 121 ° C. for 15 minutes. Thereafter, commercially available salad oil (excluding wastewater samples) and 1% of pre-cultured bacterial solution were added in a clean bench, and cultured at 160 rpm at each temperature in a rotary culture machine. After the culture, the oil and fat was extracted with n-hexane, and the weight of the remaining oil and fat was measured after removing the solvent. Similarly, a control (without adding bacteria) experiment was performed, and the difference from the weight of the fat and oil was defined as the fat and oil decomposition amount.

(Table 3 Oil and Fat Single Medium) =================================== Yeast Extract 0.01% Ammonium sulfate 0.2% dipotassium hydrogen phosphate 0.09% potassium dihydrogen phosphate 0.06% magnesium sulfate heptahydrate 0.02% calcium chloride dihydrate 0.005% iron (II) sulfate heptahydrate 0.002% pH 7.0 with distilled water Preparation ===================================

(Table 4 Artificial drainage) =================================== Peptone 0.25% Ammonium sulfate 0.25% Dipotassium hydrogen phosphate 2.5% pH 7.0 Prepared in tap water ==========================================

As shown in FIGS. 1 (A), 1 (B), and 1 (C), the optimal conditions for fat and oil degradation by the present bacteria in an LB medium are as follows:
It turns out that it is 25 degreeC and pH7.2. In addition, the fat and oil decomposition rate was higher as the fat and oil concentration was lower, and the fat and oil decomposition rate was 24% after 24 hours in the case of 0.67 g-fat / L-medium. In addition, this bacterium was able to decompose oils and fats with high efficiency even at a high oil and fat concentration of 51 g-oil / L-medium.

Further, FIG. 2 shows that 25 ° C., pH 7.2, 51 g-fat / L
-Shows the change over time and the growth curve of fat and oil decomposition in the medium. The doubling time of this bacterium is extremely fast at 0.54 hours,
After time, the amount of fat and oil decomposition was 11.5 g-fat / L-medium.
This degradation amount is about three times higher than the conventionally known bacteria (Okuda, S., K. Ito, H. Ozawa, and K. Izaki,
1991, J. Ferment. Bioeng., 71, pp424-429. ).

Table 5 shows the percentage of vegetable oil degraded by the present bacterium and commercial microbial preparations in LB medium.

It is shown that this bacterium has about 2 to 5 times the degradation rate as compared with existing microbial preparations.

[0060] *: Cannot be measured due to difficulty in extraction. Conditions: LB medium (pH 7.
2), 25 ℃, commercial salad oil 5ml

Further, Table 6 shows the fat and oil decomposition rate of the present bacteria in various wastewaters. It is shown that this bacterium is applicable to a relatively low-contamination wastewater such as a fat and oil single medium and artificial wastewater. In addition, oil contained in certain wastewater
It was also shown to degrade 48% in 24 hours.

(Table 6 Results of wastewater treatment by this bacterium) ============================================ =============================== Treatment solution pH TOD (ppm) Oil concentration (g / L) Decomposition amount * Decomposition rate (%) Inorganic medium 7.0 1180 4.40 0.139 3.16 Artificial drainage 7.0 4950 4.43 1.70 38.5 Artificial drainage 7.0 48.12 3.57 7.41 LB medium 7.2 25300 4.22 2.51 59.4 LB medium 7.2 9.47 4.48 47.3 A certain restaurant drainage 5.9 1730 4.64 2.40 48.4 A certain restaurant Drainage 7.2 0.271 0.131 48.3 Certain type B restaurant drainage 4.3 740 0.378 0 0 Food factory 5.1 1840 2.31 0 0 ============================ ======================================== * :( g-oil / L / day )

From the above results, it was determined that this bacterium was extremely effective as an oil / fat-decomposing microorganism preparation. Further, the bacterium has lipase activity and biosurfactant producing ability.

[0064]

As described above, the microorganism of the present invention has an excellent effect of decomposing fats and oils. Therefore, for example, by using the wastewater treatment equipment and apparatus for treating wastewater containing fats and oils, the removal of fats and oils from the wastewater can be performed with high efficiency and safety.

The wastewater treatment apparatus of the present invention optimizes the action of the microorganisms for decomposing and removing fats and oils, and enables safe and efficient removal of fats and oils from wastewater.

[Brief description of the drawings]

FIG. 1. Temperature (A), p given to fat and oil decomposition by OD-1 strain
It is a figure which shows the influence of H (B) and fats and oils concentration (C). Here, ● indicates the decomposition rate, and Δ indicates the amount of fat and oil decomposition.

FIG. 2 is a graph showing the change over time of fat and oil degradation by the OD-1 strain. Here, ● indicates the amount of fats and oils decomposed, and X indicates the dry weight of bacterial cells.

Continuation of the front page (56) References JP-A-2-291259 (JP, A) JP-A-61-28397 (JP, A) JP-A-10-34130 (JP, A) JP-A 7-110047 (JP) (A) JP-A-6-170377 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) WPI (DIALOG) BIOSIS (DIALOG)

Claims (5)

(57) [Claims] 1. A novel microorganism, S. saprophyticus OD-1 (FERM), which is degradable to animal and vegetable oils and fats.
P-17201). 2. A method for treating wastewater, wherein the method is performed using the microorganism according to claim 1. 3. A method for producing an oil-decomposable substance, comprising culturing the microorganism according to claim 1, and separating and purifying a novel oil-decomposable substance from the culture solution. 4. A microbial preparation containing the microorganism according to claim 1 for decomposing fats and oils. 5. A wastewater treatment apparatus for treating wastewater using the microorganism according to claim 1, comprising: an air diffuser for blowing air into the wastewater; and a trap for preventing the microorganism from flowing out. A wastewater treatment device comprising: