JP3013990B2 - New strain resistant to carbon dioxide and SO2 and method for immobilizing carbon dioxide using the new strain - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to carbon dioxide and
Relates novel strain and method for immobilizing carbon dioxide using a novel strain having a resistance to SO _2, more particularly, a high concentration of carbon dioxide and the new strain Chlorella resistance and excellent for _{SO 2 (Chlorella) KR-1} (KCTC0426BP )
And a method of stably immobilizing carbon dioxide directly from combustion gas discharged in industry using this new strain,
It relates to the use of the new strain as livestock feed.

[0002]

2. Description of the Related Art Recently, the problem of global warming due to the greenhouse effect of carbon dioxide generated due to the use of fuel has become serious, and there has been an emphasis on methods of removing or recovering carbon dioxide emitted to the atmosphere. Research is being conducted. All plants on earth have the property of absorbing carbon dioxide as a source of solar energy and synthesizing and growing organic matter, which is called "photosynthesis". Therefore, in Europe's major developed countries, forests are greened to efficiently capture carbon dioxide and utilize forest resources for various purposes. When utilizing microalgae, which are phytoplankton that live in water, the rate of immobilization of carbon dioxide per unit area can be increased several times to several tens of times compared to cultivating higher plants such as trees. With the fact that the facts have been revealed, research for culturing microalgae to capture carbon dioxide is actively progressing.

Further, if carbon dioxide can be recovered by directly supplying industrial exhaust combustion gas to a microalgae culturing apparatus, the process can be simplified, installation and operation costs of the apparatus can be greatly reduced, and economical costs can be reduced. Benefit. However, in order to practically apply the method of directly supplying industrial exhaust combustion gas to the microalgae culture apparatus, there are some problems to be solved. A typical example is the concentration of carbon dioxide in the combustion gas. While the concentration of carbon dioxide in the LNG combustion gas is the lowest value of about 10%, the concentration of carbon dioxide in the combustion gas discharged from a cement factory using anthracite as a fuel is about 20% and the highest. Value. At present, it is known that the growth of most of the general microalgae species deposited in the strain bank is impaired if the concentration of carbon dioxide is 5% or more [J. Gen.
Microbiol., 130 (1984), pp.2833-28.
38], the concentration of carbon dioxide is too high for general microalgae species to have the activity of immobilizing carbon dioxide in the combustion exhaust gas.

[0004] As a result of research for separating microalgae species having excellent growth activity under the condition of high concentration of carbon dioxide, high growth activity was obtained at the concentration of carbon dioxide (20% or less) in the combustion gas. An excellent microalgal species showing good
ar. Biotechnol., 1 (1993), pp. 21-25;
Kor. J. Chem. Eng., 15 (1998), pp. 449-
450; Japanese Patent Application No. 3-313852 (1991)]. In addition to the high carbon dioxide concentration in the combustion gas, a large amount of exhaust gas (200 to 300) which impairs the growth activity of microalgae is generated when a fuel containing sulfur components (anthracite, heavy oil, etc.) is burned.
toxic components (examples of ppm): SO _x, because it contains NO _x, etc.), by culturing the microalgae, it is impossible to immobilize the carbon dioxide directly from the factory exhaust gas. In particular,
SO _x has fatal toxicity to microalgae, and it has been reported that most microalgal species die when the concentration of SO _x is about 50 ppm [Energy Convers, Mgmt.,
36 (1995), pp. 713-716; Energy Conve
rs, Mgmt., 36 (1995), pp. 689-692].
Thus, by utilizing the microalgae, to immobilize carbon dioxide in the combustion gas containing SO _x has established a flue gas desulfurization process, to a concentration microalgae haves growth activity SO
The concentration of _x must be reduced or microalgal strains with high resistance to SO _x must be isolated or culture techniques established. However, the flue gas desulfurization process is a large-scale process, is restrictedly operated in large-scale industries such as power plants, and is difficult to apply due to high operating costs. The combustion gas is injected directly into the culture reactor of microalgae, in order to fix the carbon dioxide, the development of technologies to enhance the resistance to SO _x securing or microalgae strains with good resistance to SO _x Is required.

[0005] In the case of a strain having excellent resistance to SO _x , the maximum concentration at which it can grow is 50 ppm [Japanese Patent Application No. Hei.
-41397 (1993)]. The only microalgae species with growth activity in SO _x under conditions of high concentration of 50ppm or more in the patent application the strains to date, Synechocystis (Synech
ocystis) strain [Japanese Patent Application No. 6-196663 (199).
4)]. Assuming that SO ₂ dissolved in water and converted to sulfite exerts toxicity, a growth test of microalgae was performed while changing the concentration of sulfite in the culture medium for cell culture. As a result, when the concentration of sulfite was 6 mM, Has been reported to grow normally. Based on this, SO equivalent to a sulfurous acid concentration of 6 mM
Back-calculation of the concentration of the _two gases presumed that it would be resistant up to a SO ₂ concentration of 600 ppm, and it has been reported that microalgae species (Synechocystis strain) have strong resistance to SO _x , but this is actually SO _x It does not provide evidence of resistance to Such analyzes, when fixing the carbon dioxide from the combustion gas using a microalgae, main factors on the toxicity is SO _2, is supported by the finding that not SO ₃ [Proceedings of 212th ACS National Co
nference, 41 (1996), pp. 1391-139
6]. Synechocystis is a saltwater microalgal species, but most of the industry is located inland. Inland, freshwater is easier to use than saltwater, so research on isolation of freshwater strains is required. However, freshwater strains are reserved until now because resistance to weak SO _x, SO _x
Carbon dioxide cannot be immobilized directly from gases containing

[0006] While it would be practically most feasible to establish a cultivation technique that would allow microalgae to have a sufficiently strong resistance to SO _x , there has been no work done in this area to date. There is no. Before injecting the combustion gases to the reactor, only it was attempted that to remove the SO _x in the combustion gas in advance by placing the scrubber (prescrubber), slightly delayed kill time of microalgae, fundamental Proceedings of 212th ACS N
ational Conference, 41 (1996), pp.1391
９６1396]. If the technology to reduce the toxic effect of SO _x can be established, the combustion gas can be directly supplied to the carbon dioxide culture device, and the carbon dioxide immobilization process using microalgae is economical accordingly. Other preferable properties of microalgae species that immobilize carbon dioxide from industrial exhaust gas include stability against pH and temperature changes, high-speed growth under high-concentration culture conditions, and the like. Of particular importance is the resistance of microalgae to high temperatures. That is, a tubular photobioreactor, which is attracting attention as a post-microalgae culturing system, must be equipped with a cooling facility because the temperature inside the reactor becomes extremely high when culturing outdoors in summer. Therefore, stability of microalgae to high temperatures is important in order to reduce cooling costs.

Further, microalgae produced using carbon dioxide as a nutrient source are known to be rich in carbohydrates, lipids, proteins, etc., and their use as livestock feed has been studied [Energy Convers, Mgmt. ., 36 (199
5), pp. 713-716]. The technology of cultivating microalgae, removing carbon dioxide and converting it to organic matter and using it as livestock feed is a very promising technology that can simultaneously solve the problem of environmental pollution and food shortage due to carbon dioxide.

[0008]

Under these circumstances, the present inventors conducted research to secure microalgal strains resistant to high concentrations of carbon dioxide gas. The present invention has been completed by isolating microalgae species having high resistance to carbon and SO _x from the natural environment and securing a technique capable of reducing the toxic effect of SO _x . An object of the present invention is to solve the problem of environmental pollution by culturing a novel microalgae, and recovering and immobilizing carbon dioxide from gas discharged from industry. At the same time, it is an object of the present invention to provide a method for solving food shortages by utilizing microalgae produced by the process as livestock feed.

[0009]

SUMMARY OF THE INVENTION The present invention, chlorella with excellent resistance to carbon dioxide and SO ₂ (Chlorell
a) It relates to a new strain of KR-1 (KCTC0426BP). In addition, the present invention, carbon dioxide chlorella (C
hlorella) KR-1 (KCTC0426BP) strain. The present invention also relates to Chlorella KR-1 (KCTC0426BP)
The present invention relates to livestock feed comprising a strain or a culture obtained by culturing the strain. As mentioned above,
Novel strain of the present invention, 10% to 70% of a high concentration of carbon dioxide, it is possible to grow even under conditions of SO ₂ in the 0 to 100 ppm, the carbon dioxide from the combustion gas containing SO _x with novel strain Can be directly immobilized.

[0010]

DETAILED DESCRIPTION OF THE INVENTION Chlorella KR-1
The method of immobilizing carbon dioxide on the (KCTC0426BP) strain is as follows. For industries that use LNG fuel, does not include the SO _x are toxic components in exhaust gas, when the passed directly into the culture reactor microalgae without separate pretreatment step the gas, chlorella (Chlorella ) KR-1
Absorbs carbon dioxide as a nutrient source and emits oxygen. Compared to physicochemical carbon dioxide immobilization methods that operate at high temperatures and pressures and use high catalysts and additives (eg, hydrogen), this reaction occurs under normal temperature and pressure conditions, The necessary light energy utilizes sunlight, and the energy required for the operation of the process is required only when the culture solution is stirred, so that the energy consumption is extremely low and the process is simple. That is, the cost and the operating cost required for the construction of the process equipment are extremely low, so that the practical use is extremely easy.

When the concentration of SO _{2 in} the combustion gas discharged in an industry using a general fuel containing a sulfur component is 100 ppm or less, the SO ₂ is directly passed through a microalga culture reactor without performing another pretreatment step. And Chlorella K
R-1 absorbs carbon as a nutrient source from carbon dioxide and emits oxygen. When the concentration of SO ₂ is 100 ppm or more, the combustion gas is started to be injected into the reactor under the condition that the concentration of the inoculum is maintained at 0.1 g / l and the flow rate of the supplied gas is maintained at 0.5 vvm, and the strain is maintained for 9 hours. By maintaining the pH of the culture solution at 7 and adjusting the flow rate of the combustion gas flowing into the culture medium to 0.05 vvm, carbon dioxide in the strain can be more efficiently immobilized.

In the case of NO _x, the concentration of the inoculated cells is 0.5 g / l.
0.5 vvm gas flow, 300 ppm NO
Immobilization can be performed directly under the conditions described above. Chlorell
a) The flow rate of the combustion gas supplied to the culture medium for immobilizing carbon dioxide in the KR-1 is 0.05 to 0.5 vvm, preferably 0.05 to 0.1 vvm. On the other hand, new strains cultured using carbon dioxide as a nutrient source are rich in carbohydrates, lipids, proteins, etc.
The new strain is mixed with a small amount of amino acids, dried and pulverized, and used as livestock feed. The present invention will be described by the following examples, but the present invention should not be construed as being limited to these examples.

[0013]

EXAMPLES Example 1 Isolation and Culture of New Strains Samples were collected from rivers and soil in Yeongwol region, Korea. 20
The sample collected in a 0 ml test tube and the MBM liquid medium shown in the following Table 1 were placed, and cultured in place for about 3 weeks. A mixed gas of carbon dioxide (carbon dioxide 10%, air 90%) is supplied at a flow rate of 100 ml / min, a fluorescent lamp is used as a light source, and the illuminance is adjusted to about 90 μmol / m ² -sec on the surface of the culture test tube. After that, intensive culture was performed. 1 ml of the pre-cultured sample was placed in a 30 ml test tube containing 10 ml of sterilized distilled water, and stirred to disperse the microalgal cells. With a micropipette
A 0.1 ml sample was taken and mixed in a test tube of sterile distilled water. After repeating this process 5 times, a sample of about 0.1 ml was taken from each test tube, and inoculated into a Petri dish containing a Detmer medium having the composition shown in Table 2 below, and 25-27.
Temperature of 50 ° C. and 50 μmol / m ² -sec.
Stationary culture was performed for 3 weeks. When microalgae colonies appear,
100ml with platinum loop (platinun loop) while checking by microscopy
Was transferred to a 250 ml Erlenmeyer flask containing MBM medium, and the main culture was started.

[0014]

[Table 1] Composition of liquid culture medium (MBM) for culturing microalgae Content of KNO ₃ 250 mg MgSO ₄ .7H ₂ O 75 mg KH ₂ PO ₄ 75 mg K ₂ HPO ₄ 175 mg NaCl 25 mg CaCl ₂ .2H ₂ O 10 mg FeSO _{4 · 7H 2 O 2mg a-} 5 solution 1ml distilled water 1 liter pH 4.5 to adjust a-5 solution _{composition: H 3 BO 3 286mg, MnSO} 4 · 7H 2 O 250mg, ZnSO 4 · 7H 2 O 22.2mg , CuSO ₄ ・ 5H ₂ O 7.9mg, Na ₂ MoO ₄ 2.1mg, distilled water 1 liter

[0015]

TABLE 2 Detmer composition Ingredient including weight Ca (NO ₃₎ of the medium _{2 1,000mg KCl 250mg MgSO 4 · 7H} 2 O 250mg KH 2 PO 4 250mg FeCl 3 2mg agar (agar) 15 g H ₂ O 1 liters

Example 2 Mycological Properties of the Isolated Bacterium The growth characteristics of the isolated microalgae under the culturing conditions (concentration of carbon dioxide in the supplied gas, temperature, pH, etc.) were examined.
A test tube with a length of 60 cm is filled with 200 ml of the M4N medium of Table 3 enriched in essential nutrients, inoculated with the isolated strain, and then illuminated with a fluorescent light having an illuminance of 100 μmol / m ² -sec. A strain growth experiment was performed.

[0017]

Table 3 Composition of M4N medium Component content KNO ₃ 5 g MgSO ₄ .7H ₂ O 2.5 g KH ₂ PO ₄ 0.003 g FeSO ₄ .7H ₂ O 1 ml A-5 solution 1 liter distilled water pH 4.5 the adjustment a-5 solution _{composition: H 3 BO 3 286mg, MnSO} 4 · 7H 2 O 250mg, ZnSO 4 · 7H 2 O 22.2mg, CuSO 4 · 5H 2 O 7.9mg, Na 2 MoO 4 2.1mg, distilled water 1 liter

The microbiological properties of the isolated microalgal strain of the present invention were compared with those of a known Chlorella sp. Strain. The results are shown in Table 4 below.

[0019]

[Table 4] Classification of mycological properties Chlorella sp. Chlorella KR-1 ( KCTC0426BP ) Morphological properties Size: 2 to 10 μm Size: 3 to 7 μm Shape: Spherical Shape: Spherical culture properties Optimal pH: 4.0 to 6.0 Optimum pH: 4.0 ~ 6.0 Optimum temperature: 25 ℃ ~ 35 ℃ Optimum temperature: 25 ℃ ~ 40 ℃ Maximum growth rate: Maximum growth rate: 0.6g cells / 1-day 0.8g cells / 1-day physiological properties the optimum concentration of CO _2: optimum concentration of 5% CO _2: if the 10% (CO ₂ concentration at 10%, if 30% concentration of (CO _2, the fixed rate of the CO ₂ is optimal conditions CO killing CO ₂ at ₂ immobilized activity to supply 15% of the CO ₂ containing 80%) 15% 100ppm of SO ₂ containing 50ppm of SO ₂ when it is 50%) when a matter optimum When supplying CO2, the CO ₂ immobilization activity is 50% of the optimal conditions

As shown in Table 4 above, the new strain Chlorella KR-1 (KCTC0426B) of the present invention was used.
P) is physiologically different from common Chlorella sp. The mycological properties of the new strain of the present invention will be described in detail with reference to FIGS. As can be seen from the micrograph of FIG. 1, the isolated microalgae of the present invention are spherical and have a size of about 3-7 μm. FIG.
Chlorella (C) in the presence of low concentrations of carbon dioxide, below 0%
hlorella) KR-1 (KCTC0426BP) growth rate. Chlorella KR-1 (KCTC0
426 BP) has the fastest growth rate when 10% carbon dioxide is supplied at 1 vvm. When cultured for 7 days, it grows at a high concentration corresponding to about 30 times the initial inoculum amount, and the productivity of cells and the rate of carbon dioxide removal per unit time are as follows:
They are 0.8 g cells / 1-day and 1.6 g carbon dioxide / 1-day, respectively.

FIG. 3 shows Chlorella KR-1 (KC) in the presence of high concentrations of carbon dioxide of 10-70%.
TC0426BP). As the concentration of carbon dioxide increases to 10%, 30%, 50%, 70%
The growth rate decreases proportionately, but the growth rate remains high. In particular, according to the facts reported to date, it is known that the supply of 70% carbon dioxide completely stops the growth of all microalgal strains,
When the strain isolated according to the present invention is cultured for 7 days under a supply condition of 70% carbon dioxide, the initial inoculum cell amount is about 1
It becomes clear that the resistance is tripled, which means that the resistance to the high concentration of carbon dioxide is very excellent. FIG.
Chlorella KR-1 (KCTC)
0426BP) and the growth characteristics of the isolated strain depending on the pH of the culture medium. Chlorella KR
-1 (KCTC0426BP) shows a rapid growth rate at pH 4.0 to 6.0, and has a relatively wide optimum pH range. But,
If the pH is less than 4, it will die, which is due to its resistance to pH, Chlorella, a microalgal species patented by Watanabe et al.
orella) HA-1 [Japanese Patent Application No. 3-313852 (199)
1)].

FIG. 5 is a graph showing a change in temperature due to a change in chlorella.
la) shows the growth rate of KR-1 (KCTC0426BP), and shows the growth characteristics depending on the culture temperature. Although the growth rate at 20 ° C. is somewhat slow, the growth rate does not change so much up to 40 ° C., so that the Chlorella KR-1 of the present invention can be used.
(KCTC0426BP) strain is a mesophilic microorganism (mesophile).
ic microorganism). Further, even at a temperature of 40 ° C., although the growth rate is slightly slowed down, the growth proceeds immediately without a separate adaptation time. It can be seen that the strain of the present invention is more resistant to high temperatures than the Chlorella bacterium isolated from Hanagata et al. Has an adaptation period of 5 days under the same temperature conditions. [Phytochemistry,
31 (1992), pp. 3345-3348]. When culturing microalgae in a sealed tubular reactor using summer sunlight, the temperature of the medium may exceed 40 ° C., so that resistance to high temperatures seems to be very important.

Example 3 Identification and Nomenclature of New Strains The microalgae of the present invention have high concentrations of carbon dioxide and SO ₂
Morphology and optimal temperature, pH, except that it is resistant to
Considering the characteristics of chlorella (Chlorella)
belongs to sp. Chlorell (Chlorell)
a) Named KR-1 (KCTC0426BP), 19
On January 16, 1998, it was deposited at the Genetic Engineering Center in the Institute of Biotechnology, Korea Institute of Science and Technology. The accession number is KCTC0
426 BP.

Example 4: Chlorella KR-1
Of SO in combustion gas_x And NO_x The toxic effects of
SO for quantitative measurement _x And NO_x SO, the main component of_Two 
Uses synthesis gas produced by adding a certain concentration of NO and NO
And performed a growth experiment of Chlorella KR-1
Was. FIG. 6 shows SO in the combustion gas._Two Chlorella depending on the concentration of (C
hlorella) It is a growth curve of KR-1. Chlorella
ella) In the case of KR-1, 60 ppm SO_Two And 15%
When supplying gas containing carbon dioxide at a flow rate of 0.5 vvm
Growth rate supplies only carbon dioxide gas of the same concentration
It is equivalent to about 70% of the time, but the initial amount of bacteria is 4 days
The amount was 20 times as high as that of the amount, and showed high growth activity. 100ppm
SO_Two Culture for 4 days even when supplying gas containing
, The cell growth reached 14 times. However, 150 ppm
SO_Two The gas containing was immediately killed. Therefore,
Microalgal species reported so far [J. Mar. Biotechno
l., 1 (1993), Japanese Patent Application No. 3-313852 (19)
91), compared with Japanese Patent Application No. 5-41397 (1993)].
And SO_Two Strong resistance to

FIG. 7 is a growth curve of Chlorella KR-1 according to the concentration of NO in the combustion gas. chlorella
(Chlorella) KR-1 is 100 ppm NO and 15%
When a gas containing carbon dioxide was supplied at a flow rate of 0.5 vvm, normal growth activity was exhibited, but when a gas containing 300 ppm of NO was supplied, it died. Therefore, since the flow rate of the combustion gas has a very important effect on the growth of the strain, the flow rate of the combustion gas is preferably 0.05 to 0.1 vvm. FIG. 8 shows the pH control of Chlorella KR-
1 is a graph showing the effect of improving the resistance to SO ₂ of No. 1;
This is the result of a growth experiment of KR-1 while supplying a gas containing 250 ppm of SO ₂ at a flow rate of 0.5 vvm. 250ppm
Strain Chlorella (Chlorella) KR-1 has been killed, KR-1 culture medium by addition of 1N NaOH solution in the culture the initial (9 hours started to culture) when only the supplied carbon dioxide gas containing SO ₂ in Is adjusted to 6-7,
Normal growth activity of KR-1 could be maintained. Medium
It can be seen that by adjusting the pH, the resistance of KR-1 to SO ₂ can be increased. Thus, by utilizing the KR-1, thereby completing the method for immobilizing carbon dioxide directly from the general combustion gas containing a large amount of SO _x.

FIG. 9 shows the relationship between the flow rate of gas and chlorella.
ella) is a graph showing the effect of improving the resistance of KR-1 to SO _2, which is the result of a growth experiment conducted while supplying a gas containing 250 ppm of SO ₂ . When the flow rate of the gas supplied to the microalgae culture device was maintained at 0.3 vvm, KR-1 died, but when the flow rate of the supplied gas was 0.05 vvm, normal growth activity was exhibited. Therefore, by reducing the flow rate of feed gas, Chlorella (Chlorella) without adversely affecting the growth rate of the KR-1, it was possible to improve the resistance to SO _2. FIG. 10 is a graph showing the effect of improving the resistance to NO by the amount of inoculated cells. The initial amount of inoculated cells was increased to 0.5 g / 1, and the effect of improving the resistance to SO ₂ and NO was examined. As can be seen from FIG.
when supplying a gas containing ppm of SO _2, Chlorella (Chlorel
la) KR-1 died, but showed normal growth activity when gas containing 300 ppm NO was supplied. Therefore, increasing the initial cell mass has the effect of improving the resistance to NO.

Example 5: Immobilization of carbon dioxide In an experiment of immobilizing carbon dioxide outdoors using sunlight, when an 8 liter reactor (10 cm in diameter) was operated, Chlorella was used. KR-1 (KCTC0
426 BP) has a maximum cell productivity of about 0.8 g cells / 1-da.
y, the maximum cell concentration after 7 days of cultivation 4.
5 g / l were reached. This value is about 2.5 times higher than the maximum cell productivity of 0.33 g / 1-day obtained by culturing Chlorella pyrenoidosa under the same conditions. When the light use efficiency is increased by reducing the diameter of carbon dioxide, the carbon dioxide immobilization speed is expected to be increased by about 10 times or more.

Example 6: Production of livestock feed Chlorella KR-1 (KCTC0426B)
P) When only the strain is used as feed, after the drying and pulverization steps, 0.05 to 0.3% by weight amino acid is added, mixed and used as feed. Chlorella (Chl
orella) KR-1 (KCTC0426BP) was dried in the open air without further treatment, ground in a mortar, and passed through a 10-mesh mixture with the existing blended feed at a weight ratio of 1: 9 to be used as a feed. .

[0029]

EFFECT OF THE INVENTION Chlorella KR-1 of the present invention
(KCTC0426BP) to directly fix carbon dioxide from the combustion exhaust gas of energy-intensive industries (thermal power plants, steelworks, etc.) that emit large amounts of carbon dioxide, which is a major cause of global warming Can be. In particular, in the immobilization of carbon dioxide using the new strain of the present invention, carbon dioxide can be directly removed in the case of combustion exhaust gas of clean fuel (LNG). General fossil fuels containing large amounts of SO _x and NO _x (such as anthracite or heavy oil)
In the case of combustion gas, the culture conditions (pH, gas flow rate, concentration of inoculated cells, etc.) of the new strain are appropriately adjusted to
It can be directly injected without any processing steps to reduce the concentration of SO _x and NO _{x in} the combustion gases, resulting in reduced operating costs and economic benefits of the process. In addition, Chlorella KR-1 (K
CTC0426BP) and its culture can be used as livestock feed and contribute to solving the problem of food consumption.

[Brief description of the drawings]

FIG. 1: Chlorella KR-1 (KCTC04
26BP).

FIG. 2. Chlorella KR-1 (KCTC0426B) in the presence of low concentrations of carbon dioxide of 10% or less.
It is a growth curve of P).

FIG. 3. Chlorella KR-1 (KCTC0426) in the presence of high concentrations of carbon dioxide of 10-70%.
9 is a growth curve of (BP).

FIG. 4. Chlorella KR-1 by pH change
It is a growth curve of (KCTC0426BP).

FIG. 5: Chlorella KR-1 by temperature change
It is a growth curve of (KCTC0426BP).

FIG. 6: Chlorella according to the concentration of SO _{2 in} the combustion gas
ella) Growth curve of KR-1 (KCTC0426BP).

FIG. 7: Chlorel according to NO concentration in combustion gas
la) Growth curve of KR-1 (KCTC0426BP).

FIG. 8: Chlorella KR-1 by pH adjustment
Is a graph showing the effect of improving the resistance to SO ₂ in (KCTC0426BP).

FIG. 9: Chlorella K by adjusting gas flow rate
It is a graph showing the effect of improving the resistance to SO ₂ of R-1 (KCTC0426BP).

FIG. 10 is a graph showing the effect of improving the resistance to NO by the amount of inoculated cells.

Continuation of the front page (51) Int.Cl. ⁷ Identification code FIC12R 1:89) (72) Inventor Jin Screen Korea 305-333 Daejeon Yusn-Quu Eun-Dong Hanbit 110-1401 Apartment 99 (72) Invention Person Dong Dong Sun Korea 305-340 Daejeon Youth-Nook Dol-Yong Dong Jongkong Apartment 1-404 (72) Inventor Jeong Lee South Korea 220-122 Gangwon-de-Wonju Taejang-2dong 1035 -6-2-3 (72 ) Inventor Thorn Chur Parc Republic of Korea 305-390 Daejeon Eusung-k Jammin-Don 106-1702 Expo Apartment 464-1 (56) References JP-A-5-304945 (JP, A) JP-A-7-313141 (JP) , a) JP flat 8-9963 (JP, a) JP flat 8-116965 (JP, a) (58 ) investigated the field (Int.Cl. ^7, DB name) C12N 1/12 BIOSIS (DIALO ) WPI (DIALOG)

Claims (8)

(57) [Claims] 1. Chlorella KR-1 (KCTC0426B) having resistance to carbon dioxide and SO ₂
P). 2. A combustion gas containing carbon dioxide is converted to chlorella (C).
hlorella) A method for immobilizing carbon dioxide, comprising supplying the culture solution of a KR-1 (KCTC0426BP) strain and immobilizing carbon dioxide in the strain. 3. The method for immobilizing carbon dioxide according to claim 2, wherein the flow rate of the combustion gas is 0.05 to 0.5 vvm. 4. The concentration of carbon dioxide in the combustion gas is 10 to 10.
The method for immobilizing carbon dioxide according to claim 2 or 3, wherein the carbon dioxide content is 70%. 5. The concentration of SO _{2 in} the combustion gas is from 0 to 250 pp.
The method for immobilizing carbon dioxide according to claim 2 or 3, wherein m 2. 6. The method according to claim 2, wherein the combustion gas is a combustion gas of a clean fuel or a combustion gas of a fossil fuel. 7. The method for immobilizing carbon dioxide according to claim 2, wherein the pH of the culture solution of the strain is 6.2 to 7. 8. Chlorella KR-1 (KCT
C0426BP) A livestock feed comprising a strain or a culture obtained by culturing the strain.