JP3012469B2 - Reagent container used in chemiluminescence measurement device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a reagent container used in a chemiluminescence measuring apparatus (hereinafter, referred to as a reagent container).

[0002]

2. Description of the Related Art For example, a chemiluminescent enzyme immunoassay device (chemiluminescent EIA device) is used as a device for performing a blood test to measure the concentration of tumor markers and hormones in blood.
There is. As a reagent for causing a chemiluminescent reaction in the chemiluminescent EIA device, a substrate solution (including a luminescent substrate), a luminol solution, a microperoxidase (mP
OD), and liquid luminescence reagents such as enhancers as luminescence intensity enhancers. When these reagents are used in the apparatus, they are generally housed in a hard polyethylene container (polytank), and are configured to be guided through a transparent pipe made of tetrafluoroethylene resin.

[0003]

However, as described above, when a reagent is stored in a plastic tank, the air space in the plastic tank increases during the process of using the reagent, and for example,
The composition changes due to the incorporation of CO _{2 in} the air, the propagation of microorganisms, and the like, whereby the pH value changes, causing phenomena such as a decrease in chemiluminescence ability and an increase in blank value generation. In addition, the same phenomenon as described above occurs in the reagent moving in the pipe due to the influence of light in the pipe and the fixed-quantity injection unit in the apparatus. For this reason, there has been an inconvenience that a stable result cannot be obtained, for example, an error occurs in the measurement result.

SUMMARY OF THE INVENTION The present invention has been made in consideration of the above-mentioned circumstances, and has a reagent for containing and storing a reagent without damaging the composition and properties of the reagent used in a chemiluminescence measuring device such as a chemiluminescence EIA device. It is intended to provide containers.

[0005]

In order to achieve the above object, a reagent container according to the first invention of the present application has a container body for accommodating a reagent used for a chemiluminescence reaction, which is excellent in flexibility.
To prevent the reagent from contacting air.
Has excellent non-breathability and does not irradiate the reagent with external light.
Into a bag made of a material with excellent light-shielding properties
The bag-shaped container body is formed in a case having a light-shielding property and rigidity.

[0006] The reagent container of the second invention of this application is:
The container body for accommodating the reagent used for the chemiluminescence reaction is excellent in flexibility, and the reagent is not exposed to air.
Excellent non-breathability to prevent
Material with excellent light-shielding properties to prevent partial light irradiation
Bag shape to form consisting of charges, as well as accommodating the bag-shaped container body in a case having a light shielding property and rigidity, hermetically shielding and non-breathable pipe deriving the reagent into the mouth portion of the container body It is configured to be connected to.

[0007]

According to the first aspect of the present invention, the reagent container is provided with a container body for accommodating the reagent which is excellent in flexibility and which is exposed to the air.
Excellent non-breathability to prevent
Excellent light shielding properties to prevent external light from being irradiated
Formed in a bag shape made of a material, since this has a bag-shaped container body is configured to accommodate the light-shielding and a case having rigidity, of course during the storage of the reagent, in the container even in the course of use touching reagent to the air, it is rather name to or upon exposure to external light, such as the composition and properties of the reagents is not impaired.

In the reagent container of the second invention, in addition to the constitution of the reagent container of the first invention, a light-shielding and non-ventilating pipe is air-tightly connected to the mouth of the bag-shaped container body. ,
Of course during storage of the reagent, the touching reagent in and the pipe vessel into the air even during use, it is rather name to or upon exposure to external light, such as the composition and properties of the reagent is impaired There is no.

[0009]

1 and 2 show an embodiment of the first invention. In these figures, reference numeral 1 denotes a reagent container, which comprises a bag-shaped container body 2 for storing a reagent and a case 3 for housing and holding the container body 2.

The container body 2 is formed into a sealed bag body by stacking two sheets 4 and joining the periphery thereof by a method such as heat fusion, as shown in an enlarged view in FIG. It is a thing. That is, the sheet body 4 includes, for example, a polyester sheet 5 having a thickness of 10 to 20 μm, an aluminum sheet 6 having a thickness of 10 to 20 μm, and a thickness of 5 to 5 μm.
A polyethylene sheet 7 having a thickness of 0 to 100 μm is sequentially laminated to form a laminated sheet body 4 having a three-layer structure, and is extremely excellent in flexibility, light-shielding properties, and non-breathability. The two sheet members 4 having such excellent properties are joined so that the polyester sheet 5 is on the outside and the polyethylene sheet 7 is on the inside, thereby forming a closed bag-shaped container body 2.

Reference numeral 8 denotes a mouth portion formed on the upper portion of the container body 2, which comprises a mouth member 8A and a lid member 8B screwed to the mouth member 8A to seal an opening (not shown) of the mouth member 8A. Become.
Both the mouth member 8A and the lid member 8B are made of hard plastic, and are configured to maintain the airtightness in the container body 2.

The case 3 is made of a material having appropriate light-shielding properties and rigidity, such as cardboard or plastic.
For example, it is formed in the shape of a rectangular parallelepiped, and its upper surface 3A is divided into, for example, four parts. And this case 3
Below one side surface 3B, a barcode display section 9 indicating the type of reagent and the serial number is formed by a method such as printing or labeling.

The reagent container 1 configured as described above is used as follows. That is, in the container body 2,
A reagent used for the chemiluminescence reaction, for example, a luminol solution is filled in such a manner that air is not mixed therein, and quickly sealed with the lid member 8B. In this manner, the container body 2 filled with the reagent is accommodated in the case 3 with the opening 8 facing upward. When the reagent in the reagent container 1 is supplied to the chemiluminescence EIA device, a pipe or a syringe for quantitative injection is used. In this case, for the pipe, for example, ethylene tetrafluoride resin colored black or the like is used. And a syringe colored in brown is used as the syringe.

In the reagent container 1 having the above-described structure, the container body 2 for storing the reagent has non-air permeability and light-shielding properties, and the case 3 for housing the container body 2 has light-shielding properties. of touching the reagent in the container to air during storage, it is rather name to or upon exposure to external light, the composition, such as by propagation of penetration and microorganisms of CO ₂ in the air is changed, whereby the pH value Does not change and the chemiluminescence ability does not decrease or the amount of generation of the blank value does not increase, so that the composition and properties of the reagent are effectively prevented from being impaired. Since the case 3 has appropriate rigidity, even if the container body 2 is made of a flexible material, the container body 2 is held in a predetermined posture by the case 3 and protected from external pressure. And
In particular, there is no direct contact with other objects.

As described above, in order to lead the reagent to the mouth 8 of the container body 2, a pipe made of a tetrafluoroethylene resin colored black or the like is used, and a syringe colored brown is used. In the process of using the reagent,
Since the reagent does not come into contact with air or receive external light irradiation, the composition and properties of the reagent are not impaired as described above.

FIG. 3 shows the measurement of carcinoembryonic antigen (CEA ) which is one of the measurement items of the chemiluminescence EIA device when the reagent is stored in the reagent container 1 and when the reagent is stored in the conventional plastic tank. In this figure, the stability of the data is shown. In this figure, the points indicated by + symbols indicate the CEA concentration when the reagent container 1 of the present invention was used, and the points indicated by ○ symbols indicate the conventional values. Shows the CEA concentration when the poly tank was used. From this figure, it can be seen that according to the reagent container 1 of the present invention, a more stable measurement result can be obtained as compared with the conventional case.

In the above-described embodiment, the flexible container body 2 is completely housed in the rigid case 3, but instead of this, as shown in FIG. The container body 2 may be stored in the case 10 so as to be held at the mouth 8 so as not to protrude from the inside of the semi-open case 10. With this configuration, the composition and properties of the reagent are not impaired, as in the above-described embodiment. In the case of this embodiment, the container body 2 is not completely protected by the case 10, but the container body 2 is held in a predetermined state and can be easily protected.

FIGS. 5 and 6 show an embodiment of the second invention. In these figures, FIG. 1, FIG. 2 and FIG.
Since the same reference numerals shown in the above indicate the same items,
The description is omitted. In this embodiment, the first
Instead of the lid member 8B used in the present invention, a pipe 11 of an appropriate length for drawing out the reagent in the container body 2 is airtightly connected to the mouth member 8A, and 8C is provided at one end of the pipe 11. And a connection member 12 screwed to the mouth member 8A,
Is a hermetic plug provided on the other end side of.

Here, as shown in FIG. 6, the pipe 11 has an insertion pipe 11a of an appropriate length inserted through the opening 8a of the mouth member 8A and inserted into the container main body 2, and a device side (not shown). And a connection tube 11b connected to the connection pipe 11b. The connecting pipe 11b and the hermetic plug 12 are made of, for example, ethylene tetrafluoride resin colored black or the like.

It is needless to say that the reagent container 1 thus configured has the same effect as the reagent container 1 of the first invention.

The present invention is not limited to the above embodiment, but can be implemented in various modifications. For example, in the three-layer structure of the container body 2, an aluminum vapor-deposited layer having a predetermined thickness may be formed instead of the aluminum sheet 6. Further, a multilayer polyethylene film may be used instead of the three-layer structure.

[0022]

As described above, according to the reagent container of the present invention, the container body for containing the reagent is said to be excellent in flexibility.
In particular, the reagent must be kept
Excellent in temperability and the reagent is not irradiated with external light.
Into a bag made of a material with excellent light-shielding properties
And, because it is constituted by accommodating the bag-shaped container body in a case having a light-shielding property, of course during the storage of the reagent, the reagent in the container even when used process or contact with the air, outside the it is rather name to or irradiated with light, such as the composition and properties of the reagents is not impaired. Also the case
Has appropriate rigidity, so that the container body is made of a flexible material.
Even if it is composed of
Position and protected from external pressure.
Are also possible such as in particular contact with etc. Other objects directly
Absent.

When the reagent container of the present invention is used, stable measurement can be performed as compared with the case of the conventional polytank.

[Brief description of the drawings]

FIG. 1 is a perspective view showing an example of a reagent container according to the first invention of the present invention.

FIG. 2 is an exploded perspective view showing a main part of the reagent container.

FIG. 3 is a diagram for explaining the performance of the reagent container and a conventional reagent container.

FIG. 4 is a view showing another embodiment of the first invention.

FIG. 5 is a perspective view showing an example of the reagent container of the second invention of the present invention.

FIG. 6 is an exploded perspective view showing a main part of the reagent container.

[Explanation of symbols]

1 ... reagent container, 2 ... container body, 3, 10 ... case, 8 ...
Mouth, 11 ... piping.

Claims (2)

(57) [Claims] 1. A container body for accommodating a reagent used for a chemiluminescence reaction is excellent in flexibility and said reagent is
Excellent non-breathability to prevent contact with air, and
Shading to prevent external light from irradiating the reagent
A container for a reagent used in a chemiluminescence measuring apparatus, wherein the container is formed in a bag shape made of a material having excellent properties, and the bag-shaped container body is housed in a case having light-shielding properties and rigidity. 2. A container body for accommodating a reagent used for a chemiluminescence reaction is excellent in flexibility and said reagent is
Excellent non-breathability to prevent contact with air, and
Shading to prevent external light from irradiating the reagent
The bag-shaped container body is formed in a bag shape made of a material having excellent air permeability, and the bag-shaped container body is housed in a light-shielding and rigid case. A container for reagents used in a chemiluminescence measuring apparatus, wherein the pipes are connected in a gas-tight manner.