JP2968436B2 - Sample pattern reading device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample emitting light in the form of a flat plate.
More particularly, the present invention relates to a sample pattern reader for reading a weak light emission pattern emitted from a flat sample.

[0002]

2. Description of the Related Art Conventionally, analysis methods by gel electrophoresis have been often used for fractionation and structural analysis of proteins and nucleic acids of biopolymers. Since the gel electrophoresis method can appropriately analyze a very small amount of sample, when the gel electrophoresis method is used, the amount of the sample that can be obtained is often limited.
For this reason, since a limited number of samples are used in the analysis process, particularly high reliability and high detection sensitivity are required.

[0003] Therefore, conventionally, when the amount of available sample is small, the sample to be analyzed is labeled with a radioisotope, the sample is injected into a gel, electrophoresed, and then the gel is subjected to x-rays. It was read as the electrophoresis pattern of a sample by sticking it to a film or the like and exposing it, and reading the pattern of exposure with the radioactive isotope transferred to the x-ray film.

[0004] However, radioisotopes are dangerous and their handling must be strictly controlled. Therefore, in recent years, a chemiluminescence method for detecting an electrophoresis pattern with high sensitivity using chemiluminescence has been developed. In such a chemiluminescence method, a film is exposed to light by chemiluminescence due to a chemical reaction obtained by mixing a substance such as an enzyme labeled on a sample with a luminescent substrate, thereby obtaining a sample pattern.

[0005] As an example of a document reporting the development of a chemiluminescence reading method, see "C. Martin, L. Bresnick,
RR Juo. JC Voyata, and I. Bronstein 1991:
“Improved Chemiluminescent DNA Sequencing”, Bio T
echniques 8, 110-113 ". These techniques are used in principle for various experiments such as DNA base sequence determination, Southern blotting, Western blotting and Northern blotting.

In reading a sample pattern by gel electrophoresis, usually, after electrophoresis is completed, the sample is transferred from the gel onto a membrane and fixed, and a probe or the like labeled with an enzyme involved in luminescence is used. The hybridization causes the target sample fragment to emit light specifically. Regarding the mechanism of luminescence, reagents have been released from many reagent manufacturers including the above-mentioned literature, and are described in the instruction manual. In particular, since they are not directly related to the main part of the present invention described later, the explanation here is made. Is omitted.

[0007] The luminescence time of the enzyme varies depending on the type of reagent, but lasts for about ten minutes to several hours. Exposure to the film is performed by placing the membrane on which the light-emitting sample has been transferred into a transparent resin bag, and attaching a high-sensitivity film that is sensitive to visible light in a light-shielded case. Exposure is performed, for example, for about 10 to 30 minutes while adjusting the exposure time according to the above. As the high-sensitivity film here, an x-ray film or the like used for exposure with a radioactive isotope can be used. The exposed film is developed and the pattern is visually analyzed.
The image of the exposed film is read using an image reading device such as a CCD camera or an image scanner, and analyzed by various image analysis software.

By the way, in the above-described conventional technology, in the electrophoresis pattern reading by the chemiluminescence method , when the light generated by the chemiluminescence is directly read,
Since the emitted light is very weak light, it is particularly necessary to read with ultra-high sensitivity. Therefore, the cooling type C
An expensive high-sensitivity reading device such as a CD camera is required. In addition, image capture by CCD camera
Although it is flexible in that it can be freely enlarged or reduced, it is necessary to simultaneously read a standard scale such as the migration distance when comparing samples, and there is a problem that the conversion is troublesome.

On the other hand, a scanner using a one-dimensional contact type sensor has an advantage of accurately reading the distance accuracy of reading, but on the other hand, it reads with high sensitivity even when high sensitivity is required. The device has not been developed.

Although an image scanner for directly reading a film has been developed, there is a problem that it is not suitable for quantitative analysis due to the non-linearity of the film sensitivity. The method of analyzing a sample pattern using a film, once exposing a high-sensitivity film, developing, and attaching the film to a film reader, and, further, the work of reading the sample pattern is required, The series of operations is complicated, and if the exposure is not performed with an appropriate exposure time,
There is also a problem that the reproducibility and reliability of the experiment are lacking, for example, the luminescence pattern of the target sample cannot be captured or the background noise increases.

The present invention has been made in order to solve these problems, and an object of the present invention is to provide a highly quantitative, inexpensive, highly sensitive, weak light-emitting device that emits light from a flat sample. An object of the present invention is to provide a sample pattern reading device for reading a pattern. Another object of the present invention is to provide a sample pattern reading device capable of correcting and reading out a temporal change in light emission in order to improve quantitativeness.

[0012]

In order to achieve the above object, a sample pattern reading apparatus according to a first aspect of the present invention is a sample pattern reading apparatus for reading an emission pattern of a sample emitting light in a flat plate. The sample to be read is placed and the sample cover
Stage means for forming an optically sealed room by covering the sample, a light condensing means for condensing light having a light emission pattern emitted from the sample, the stage means and the light condensing means. Moving means for relatively moving the means, and divides the light of the light emission pattern condensed by the light condensing means into predetermined sections,
Light receiving means for selectively receiving the light from the compartment by one-dimensionally, and the photoelectric conversion means that converts <br/> into an electric signal the optical signal received by said light receiving means, electricity from the photoelectric conversion means Data processing means for converting a signal into a digital signal and reconstructing an image from an optical signal of a one-dimensionally selectively received section.

In a sample pattern reading apparatus according to a second aspect of the present invention, the stage means includes a mounting table member on which a sample to be read is mounted and a sample cover member. The sample mounted on the mounting table member is covered with the cover member to form an optically sealed room.

In the sample pattern reading apparatus according to the third aspect of the present invention, the light from the luminescent pattern of the sample to be read is converted to the phosphorescent or stimulable light from the sample transferred to the membrane. It is a light pattern that emits light with fluorescence or a chemical change.

Further, in a sample pattern reading apparatus according to a fourth feature of the present invention, the data processing means further includes a correction processing means for correcting a temporal change of the emission intensity.

In a sample pattern reading apparatus according to a fifth aspect of the present invention, the light receiving means includes a shutter element and a light guide for forming a predetermined section, and the sample element is controlled by the shutter element to control a section shutter. Departure
The light of the light pattern is divided by a predetermined section, the light of the divided section is selectively passed one-dimensionally, and the light is derived from a light guide and received.

Further, in the sixth sample pattern reading apparatus according to claim of the present invention further comprises a planar light source for emitting light in a plane with respect to specimen, when the flat-plate-like sample does not emit light The method further comprises causing the planar light source to emit light and reading a transmitted light pattern from the sample.

[0018]

In the sample pattern reading apparatus of the present invention having the above-described features, when reading a light emission pattern of a sample which emits light in a flat plate shape, the sample to be read is placed on the stage means . The stage means is to read
When the sample is placed, it is
To form an optically sealed room
The sample to be read is protected from external light noise.
You. Moving means, said the stage means and the focusing means are moved relative, you <br/> start reading the emission pattern of the sample. Since the light condensing means condenses the light of the light emission pattern emitted from the sample, the light receiving means divides the light of the sample pattern condensed by the light condensing means into predetermined sections. , And selectively receive light from the sections in a one-dimensional manner.
The photoelectric conversion unit converts an optical signal received by the light receiving unit into an electric signal. Then, the data processing means converts the electric signal from the photoelectric conversion means into a digital signal, and
An image is reconstructed from the optical signals of the sections selectively received in a two-dimensional manner.

The stage means here comprises a mounting table member on which a sample to be read is mounted, and a sample cover member. Then, at the time of reading the sample, the sample placed on the mounting table member is covered by the sample cover member to form an optically sealed room. Thus, even when a very weak light emission pattern is read, it is possible to read with high sensitivity by blocking noise of external light.

Further, in the sample pattern reading apparatus wherein the light emitting pattern from the sample to be read, with the phosphorescent or stimulable phosphor or chemical changes, from a sample that has been transferred to a membrane The data processing means further includes a correction processing means for correcting a temporal change in the light emission intensity. Further, the light receiving means includes a shutter element and a light guide forming a predetermined section. When receiving the light emission pattern and more control of the partition shutter by the shutter elements, originating in the sample
The light of the light pattern is divided by a predetermined section, the light of the divided section is selectively passed one-dimensionally, and the light is derived from the light guide and received.

Further, in the sample pattern reading apparatus here includes a planar light source for emitting light in a plane with respect to specimen, when the flat-plate-like sample does not emit light, light is emitted the light source Irradiate in a flat plate. Assuming that the transmitted light pattern is the light emission pattern of the sample, the transmitted light pattern from the sample can be read because it can be handled in the same manner as the light emission pattern.

[0022]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be specifically described below with reference to the drawings. FIG. 1 is a block diagram illustrating the overall configuration of a sample pattern reading apparatus according to one embodiment of the present invention. In FIG. 1, 10 is a reading device main body,
11 is a sample support, 12 is a sample cover unit, 13 is a flat light source, 14 is a reading carriage including a light receiving unit, 15 is a data processing unit, 16 is a printer, and 17 is a data storage device.

Next, the operation of the outline of the entire reference while apparatus 1 will be described in accordance with the reading operation of the light emitting pattern of the sample. Open the sample cover portion 12 in the reading apparatus main body 10, a sample of the emission pattern that has been prepared Me pre, placed on the sample holder 11, closes the sample cover portion 12. Since the light of the light emission pattern of the sample here is very weak light, as described later, the periphery of the sample cover portion 12 and the sample support base 11 has a convex portion around the upper portion of the sample cover portion 12. In addition, the periphery of the housing of the sample support base 11 on the lower side is formed as a concave portion and has a structure in which they are fitted to each other, and the sample cover portion 12 forms a room in which light does not leak from the surroundings. It has a structure to do.

The plate-shaped light source 13 is equipped on the rear surface of the specimen cover 12 (sample surface) is a planar light source for emitting light in a plane against the specimen is placed on the sample holder 11 When the flat sample does not emit light, the flat light source 13 emits light to irradiate the sample surface. Thus, even if the sample does not emit light, transmitted light path coater emissions of the sample by the plate-shaped light source 13, since the handled similarly to the light emission pattern, read it. As will be described later, the light receiving section for reading the light emission pattern of the sample has a high reading sensitivity, and the flat light source 13 can be used sufficiently even if its luminance is low. As the flat light source 13,
Here, an electroluminescent light-emitting body (EL light-emitting body) as a plane light-emitting body is used.

By the way, the light emission from the specimen, so that continued for 10 minutes or more, by the reading carriage 14 is a drive mechanism including a receiving portion therebetween, to move in the right and left direction in FIG. 1, according to the moved reading position Then, the light receiving section reads the light from the light emitting pattern. Read carriage 14
The configuration of the light-receiving section will be described later in detail.
The light emitted from the sample is condensed using a light condensing element such as a SELFOC lens array, is divided into predetermined sections using a PLZT optical shutter, and the light having a size corresponding to the sample reading section is one-dimensionally converted. The light is guided selectively to the photoelectric conversion element by a light guide, converted into an electric signal, and further converted into a digital signal and output.

The data of the digital signal of the light emission pattern thus read is sent to the data processing section 15. Then, the data is processed as image data as necessary in the data processing unit 15 to reconstruct the image of the light emission pattern, and the image is displayed on the screen of the display device.
The data is stored in the data storage device 17 as image data. This image data is also output to the printer 16. Here, as the printer 16, a printer capable of multi-tone expression is preferable.
A full-color printer having a performance of 56 gradations is used.

FIG. 2 is a sectional view of a main part for explaining the structure of the sample cover and the sample support in the main body of the reading apparatus. As shown in FIG. 2, the sample cover unit 12 includes a cover support member 21 that supports the entire cover, and a surface light emitter 22.
And the protection sheet 23. The sample support base 11 includes a sample support plate 24 made of a transparent glass plate and a housing 25 that supports the sample support plate 24. Also,
The reading carriage 14 including the light receiving unit described above is provided inside the housing 25, and the reading carriage 14 is moved in the front-rear direction on a paper surface by a driving mechanism (not shown).

The cover support member 21 on the upper side is formed in a convex portion 26 whose periphery is downward, and the casing 25 of the sample support 11 on the lower side faces the convex portion 26 in accordance with the shape of the convex portion 26. The shape around the portion to be formed is formed in the shape of the concave portion 27. The shape of the convex portion 26 and the concave portion 27 has a structure in which the sample cover portion 12 is fitted to each other when the sample cover portion 12 is closed.

Further, at the time of pattern reading in the sample that emits light, a state where the power of the surface light emitters 22 is turned off, at the time of pattern reading of the sample does not emit light, for example, at the time of pattern reading of the sample of the film, the surface light emitter as 22 when the power is put in (a state of being lit), and the path <br/> terpolymers down reading operation of the sample. This allows the sample to emit light
Both likewise read operation pattern and does not emit light pattern of the sample of is I line. Samples that do not emit light
In the case of the pattern reading will be read light pattern to <br/> transmitted as a pattern of the sample receiving unit.

[0030] When starting the pattern reading sample, closes the sample cover portion 12 by the sample to be read is placed on the sample support plate 24. When the sample cover 12 is closed, the protective sheet 23 holding the specimen, further, the convex portions 26 and concave portions 2 7 fitted, constitutes a room, such as leaking into the light from the surroundings. The reading of the emission pattern of the sample in the room is I line. The start of the reading of the light emission pattern is started by an instruction from the data processing unit 15. When reading is started, the reading carriage 14
To perform a reading operation. At this time, in the case of a film-like sample, the surface light emitter 22 is turned on, the pattern of the sample is detected by light transmitted through the sample , and the light receiving unit detects the pattern, converts the pattern into an electric signal, and reads an image.

FIG. 3 is a perspective view illustrating a driving mechanism of a reading carriage of the reading apparatus main body, and FIG. 4 is a side view illustrating a configuration of a light receiving section of the reading carriage of the reading apparatus main body. FIG. 5 is a perspective view illustrating a configuration of a light receiving unit of a reading carriage of the reading apparatus main body. 3, 4, and 5, 14 is a reading carriage, 23 is a protective sheet for holding a sample, 2.
4 is a transparent glass plate sample support plate, 30a and 30b are guide shafts, 31 is a timing belt, 40 is a flat sample, 41 is a selfoc lens array of a light receiving unit, 42
Is a PLZT optical shutter, 43 is a light guide, 44 is a light guide cap, 45 is a photomultiplier, 46
Is an electric signal line.

As shown in FIG. 3, the drive mechanism of the reading carriage is provided with a reading carriage 14 below a transparent glass plate sample support plate 24. The reading carriage 14 includes a selfoc lens array 41 and a light guide 43. , And a light receiving section including a photomultiplier 45 and the like. The reading carriage 14 is guided by guide shafts 30a and 30b, and is slidably held in right and left directions. In the driving mechanism, a part of the timing belt 31 is fixed to the reading carriage 14, and the timing belt 31 is connected to a driving stepping motor (not shown), and the driving force from the stepping motor is transmitted to the reading carriage 14. doing. Accordingly, the reading carriage 14 moves by the rotation of the timing belt 31.

In the reading carriage 14, FIG.
As shown in the figure, the SELFOC lens array 41, PLZ
A light receiving unit including a T light shutter 42, a light guide 43, a base 44, a photomultiplier 45, etc.
An optical system that reads a light pattern from the optical system is constructed. The SELFOC lens array 41 is an optical component that is a rod-shaped lens and has a different refractive index between the center and the periphery of the rod and has a function as a condensing lens, and the lenses of each rod are arranged in a one-dimensional array. The light emission pattern on the sample is one-dimensionally faithfully imaged on the opposite focus line.

The light of the light pattern emitted from the sample 40 is
The light is condensed by the SELFOC lens array 41 and forms an image on the PLZT optical shutter 42. The light from the sample that has passed through the section where the PLZT optical shutter 42 is in the transmission state is
The light reaches the light entrance of the light guide 43. The reading resolution is determined by the section of the PLZT optical shutter 42. In this example, a one-dimensional PLZT optical shutter of 16 pixels / mm is used.

As shown in FIG. 5, the light guide 43 arranges optical fibers on an array and sets the other end to one.
These are optical components bundled together. In this example, a plastic fiber having a diameter of 0.25 mm is used as the optical fiber so that light from the PLZT optical shutter 42 can be sufficiently taken in. Here, in the light receiving unit, the light from the light guide 43 is directly applied to the photomultiplier 45 via the base 44. However, when a specific wavelength is separated and read, the base 44 and the photomultiplier are used. By inserting an interference filter or colored glass between the 45, multi-color reading becomes possible. Here, the photomultiplier 45 uses a head-on type.
A side-on type can be used without any problem according to the emission wavelength distribution from the sample.

FIG. 6 is a block diagram showing the configuration of the electric system of the reading device. As shown in FIG. 6, the configuration of the electric system includes a microprocessor (CPU) 50 for executing the entire control processing, a read-only memory (ROM) 52 for storing control software, a temporary data Random access memory (RAM) 51 for storage of data and other data processing, PLZT optical shutter 42
Shutter controller 54 for controlling the motor controller 55 which controls the motor for the drive control of the read <br/> calibration Li Tsu di, motor driver 56, read A / D converter for analog-to-digital conversion data 57, a shading correction circuit 58 for correcting a fixed displacement of the optical measurement system including unevenness in the intensity of the light receiving unit and the light source, a light emitter driver 59 for controlling the surface light emitter 22, and an external data processing unit 15. And a SCSI controller 53 for controlling the interface with the controller.

FIG. 7 is a flowchart for explaining the overall operation flow of the electric system of the reading apparatus. As shown in FIG. 7, when the power is turned on, the entire operation of the electric system first initializes the entire apparatus in step 61. The work of this initialization is
Check read only memory (ROM) 52 and random access memory (RAM) 51, turn on light source
Checking the operation of the light source and the light receiving unit when turned off, initializing the interface unit of the SCSI controller 53 for controlling the interface, checking the operation of the driving system and finding the origin of the reading carriage, checking the operation of the PLZT optical shutter 42, checking the operation of the surface light emitter 22 A pattern of light emission unevenness is read.

When the initialization processing is completed, the process proceeds to the next step 62, where the host waits for a command from the host. If a command is input, the process proceeds to step 63, executes the command processing, and again executes step 62.
Return to the command waiting state.

In the case of a read command, at the time of pattern reading of the sample by the transmitted light pattern to turn on the light emitter. In the case of pattern reading of the sample by light emission pattern, remains off the light source of the surface light emitters 22,
Start reading. When reading is started, shading correction processing is performed by the shading correction circuit 58 while acquiring data for each line, and the data is sent to the data processing unit 15 through the interface of the SCSI controller 53. After transmitting one line of data, the motor is driven to start reading the next line.

In the operation of the PLZT optical shutter for each line, each pixel data is read by sequentially transmitting one pixel at a time from the reference side. However, in this case, the ratio (duty ratio) of the time in the transmission state in the whole
Is low. For this reason, in order to read the light of the emission pattern of the sample with higher sensitivity, on / off control of each pixel of the entire line of the PLZT optical shutter is controlled according to an orthogonal function, and about half of the pixels are always on. The reading is performed by controlling so as to be in the state, and the corresponding light amount value is obtained by the inverse operation.

For example, for each pixel "ON state" and "O
Assuming that the FF state is an orthogonal matrix [W] corresponding to “1” and “0”, respectively, and the emission luminance of each section on the sample is a column vector [g], the measured data is [y] as [y] ] = [W] [g] Therefore, the column vector [g] can be obtained by applying the inverse matrix of [W] from the left side, and a Walsh function is applied as the orthogonal matrix. Since the “1,0” matrix and the “1, −1” matrix are directly related by adding appropriate scaling and offset,
Walsh transform (RD Brown: A Recursive Algorithm for
Sequency-ordered Fast Walsh Transforms, IEEE-C, V
ol. C24, No. 8, P819-P822 (1977) and the like can be used.

Although the entire reading time of such a sample pattern reading apparatus is about 5 minutes, when the amount of light emission from the sample changes significantly during that time, the shading is performed so that the overall sensitivity becomes the same on average. Read while performing correction processing. First, prior to reading, for example, in this sample pattern reading apparatus, all the pixels of the optical shutter 42 are turned on, the total light emission amount of each line is read in a short time, and several reference positions are set by a user's instruction. I do.

Next, when reading is started, one of the nearby reference positions is set within a predetermined time of about 30 seconds to about 1 minute.
The light emission from the entire line is read, and the next area is read while correcting the decrease. If the attenuation of the emission time of the already values, simultaneously processing Shudi in g corrected by feeding the reader main body 10 from the data processing apparatus 15 side.

FIG. 8 is a block diagram showing a configuration of a main part of the shading correction circuit. The shading correction circuit (58: FIG. 6) captures the light guide 43 and the sensitivity unevenness of the photomultiplier in the reference area on the home position side. Then have placed Shinano sample or <br/> or, by measuring the entire surface unevenness of the surface light-emitting body 22 while moving the reading carriage of the light receiving portion, as shown in FIG. 8,
The data is stored in the writable nonvolatile memory 71 as shading correction coefficient data. When the time decay of the light emission pattern is apparent, the correction in accordance with the reading time is further multiplied according to the reading direction.

When the sample is set and the pattern of the sample is read, the data is read from the memory for correction, and A
The read data 73 subjected to the / D conversion is converted by using a table to obtain final data. Since the conversion accompanying the shading correction when reading the emission pattern of the sample is performed by a conversion method using a memory table, a correction table 72 for shading correction is provided. In the correction table 72, a value to be converted is previously written as data, and in the conversion accompanying the correction, read data 73 of the pixel data after A / D conversion is added as an address of the memory table, and the corrected data is read out. The data is sent to the data bus 70. As a result, as shown in the graph of FIG. 9, even if the read output level changes depending on the carriage direction pixel, it can be corrected.

As the luminescence pattern of the read sample, luminescence by phosphorescence, luminescence by a stimulable phosphor, or the like can be used in addition to chemiluminescence. In the case of a stimulable phosphor, light emission can be read in a transmission mode from a surface light source by coating on a very thin layer. In the case of electroluminescence, the type of wavelength of the light source can be selected to some extent. Thus, only stimulable light emission can be selectively transmitted through an optical filter set in front of the photomultiplier, and a signal can be detected.

As described above, according to the sample pattern reading apparatus of this embodiment, the reading carriage 14
Can be configured with very high sensitivity, the light source of the surface light emitter is the light source of a normal scanner (about 1000 cd).
The brightness may be sufficiently lower than that. Here, as the surface light emitter, a backlight used in a liquid crystal display or the like can be used. For example, a combination of a fluorescent tube and a diffusion plate, an electroluminescent luminous body, and the like. In this embodiment, an electroluminescent light emitting device of about 50 cd is used.

[0048]

As described above, according to the sample pattern reading apparatus of the present invention, when reading the light emission pattern of a sample , a weak light emission pattern such as chemiluminescence is read with a light receiving portion having a simple configuration. The carriage enables efficient light reception and visualization of the light emission pattern of the sample in the same manner as the simple scanner method. Further, according to this sample pattern reading device, since a planar light source (surface light emitter) is mounted, a conventional film can be read similarly. Since the planar light source (surface light emitter) has no moving part, it is possible to configure a system that is smaller and has a longer life than the conventional method of reading while moving using a fluorescent lamp or the like as a light source.

[Brief description of the drawings]

FIG. 1 is a block diagram illustrating an overall configuration of a sample pattern reading apparatus according to an embodiment of the present invention;

FIG. 2 is a sectional view of a main part for explaining the structure of a sample cover section and a sample support base in the main body of the reading apparatus;

FIG. 3 is a perspective view illustrating a driving mechanism of a reading carriage of the reading apparatus main body.

FIG. 4 is a side view illustrating a configuration of a light receiving unit of a reading carriage of the reading apparatus main body.

FIG. 5 is a perspective view illustrating a configuration of a light receiving unit of a reading carriage of the reading apparatus main body;

FIG. 6 is a block diagram showing a configuration of an electric system of the reading device;

FIG. 7 is a flowchart for explaining the overall operation flow of the electric system of the reading apparatus;

FIG. 8 is a block diagram showing a configuration of a main part of a shading correction circuit;

FIG. 9 is a graph illustrating a state in which a read output level changes depending on a carriage direction pixel.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 10 ... Reading apparatus main body, 11 ... Sample support stand, 12 ... Sample cover part, 13 ... Plate light source, 14 ... Reading carriage including a light receiving part, 15 ... Data processing part, 16 ... Printer, 17 ... Data storage device, 21 ... Cover support member, 2
2 ... surface light emitter, 23 ... protective sheet, 24 ... transparent glass plate sample support plate, 25 ... housing, 26 ... convex part, 27 ... concave part,
30a, 30b: guide shaft, 31: timing belt, 40: flat sample, 41: selfoc lens array of light receiving section, 42: PLZT optical shutter, 43: light guide, 44: base of light guide, 45: photo Multiplier, 46 ... electric signal line.

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Naganori Nasu 6-81-Ouecho, Naka-ku, Yokohama-shi, Kanagawa Prefecture Within Hitachi Software Engineering Co., Ltd. (72) Inventor Toshiaki Ito 6, Onoe-cho, Naka-ku, Yokohama-shi, Kanagawa No. 81, Hitachi Software Engineering Co., Ltd. (56) References JP-A-4-223261 (JP, A) JP-A-61-245762 (JP, A) JP-A-5-10885 (JP, A) JP-A-61-230463 (JP, A) JP-A-61-238046 (JP, A) JP-A-4-199971 (JP, A) JP-A-4-38165 (JP, U) (58) Fields investigated (Int) .Cl. ⁶ , DB name) G06T 1/00 G01N 21/64 H04N 1/04

Claims (6)

(57) [Claims] 1. A sample pattern reading device for reading a light emission pattern of a sample which emits light in a flat plate shape, wherein a sample to be read is placed and a sample cover member is mounted on the sample cover member.
An optically sealed room is constructed to cover the placed sample.
Stage means for forming, a light condensing means for condensing light of a light emission pattern emitted from the sample, a moving means for relatively moving the stage means and the light condensing means, and light condensing by the light condensing means is the light divided into a predetermined section of the light emission pattern, a light receiving means for selectively receiving the light from the compartment by one-dimensionally, that converts the optical signal received by said light receiving means into electrical signals the optical < and a data processing unit for converting an electric signal from the photoelectric conversion unit into a digital signal and reconstructing an image from an optical signal of a one-dimensionally selectively received section. A sample pattern reading device characterized by the above-mentioned. 2. The sample pattern reading device according to claim 1, wherein the stage means includes a mounting table member on which a sample to be read is mounted, and a sample cover member. A sample pattern reading device, wherein a sample mounted on a mounting table member is covered by a member to form an optically sealed room. 3. The sample pattern reading device according to claim 1, wherein the light from the emission pattern of the sample to be read is converted into phosphorescence or stimulable fluorescence from the sample transferred to the membrane, or a chemical change. A sample pattern reading device, which is a light pattern that emits light. 4. The sample pattern reading apparatus according to claim 1, wherein the data processing means further includes a correction processing means for correcting a temporal change of the emission intensity. 5. The sample pattern reading device according to claim 1, wherein the light receiving unit includes a shutter element and a light guide that form a predetermined section, and controls a section shutter by the shutter element to control a light emission pattern of the sample. A sample pattern reading device, wherein light is divided by a predetermined section, the divided light is selectively passed one-dimensionally, and the light is guided by a light guide and received. 6. A flat plate which irradiates a sample with light in a plane.
A light source, and when the flat sample does not emit light,
Light from the sample and the transmitted light pattern from the sample
Reads the pattern of a flat sample read as a turn
A sample pattern reading device, a stage means for mounting a sample to be read, a light condensing means for condensing light of a light emission pattern from the sample, and the stage means and the light condensing means relatively Move
Moving means, and the light of the light emission pattern condensed by the condensing means
Divided into sections, and selectively illuminates light from the sections in a one-dimensional manner.
A light receiving means for receiving the light for converting the optical signal received by said light receiving means into electrical signals
Varying the electric means, the electrical signal from the photoelectric conversion means into a digital signal
In other words, the image is obtained from the optical signal of the one-dimensionally selectively received section.
Data processing means for reconstructing an image.
Sample pattern reading device.