JP2936340B2 - Stabilization of enzyme aqueous solution - Google Patents
Stabilization of enzyme aqueous solutionInfo
- Publication number
- JP2936340B2 JP2936340B2 JP14878690A JP14878690A JP2936340B2 JP 2936340 B2 JP2936340 B2 JP 2936340B2 JP 14878690 A JP14878690 A JP 14878690A JP 14878690 A JP14878690 A JP 14878690A JP 2936340 B2 JP2936340 B2 JP 2936340B2
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solution
- enzyme
- amylase
- stabilization
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Description
【発明の詳細な説明】 産業上の利用分野 本発明は、酵素水溶液中のアミラーゼ活性の安定化方
法、即ち、水溶液状態で長期間の保存が可能で、アミラ
ーゼ活性の低下を押さえ安定性を改善されたアミラーゼ
水溶液に関する。The present invention relates to a method for stabilizing amylase activity in an aqueous enzyme solution, that is, a long-term storage in an aqueous solution state, and a decrease in amylase activity is suppressed to improve stability. To an aqueous amylase solution.
従来の技術 現在使用されている酵素剤は、殆どが粉末製品で、酵
素水溶液に比べ使用時に溶解が必要であるなど取り扱い
が不便で、粉塵の問題も残る。なかでも、使用時におけ
る最も大きな問題は近年の飛躍的に進歩した機械化によ
り、粉末状では、対応出来なくなり、酵素を大量に使用
する業界では、作業ラインに組入れやすい水溶液が要望
されている。しかし、酵素製品は粉末状態では安定であ
るが、水溶液状態では非常に不安定で、保存性が悪い。
特にアミラーゼ水溶液は、液温度が上昇すると、容易に
アミラーゼ活性が急速に低下し、失活してしまう。しか
も、水溶液自体を長時間放置すると、腐敗して使用でき
ない問題がある。従来、酵素水溶液の安定保存として、
食塩、アルコール類等を添加しているが、防腐効果はあ
っても酵素活性の安定化を改善させ得るものでない（公
開特公昭63−17690号公報参照）。その他、種々安定化
剤について研究されているが、アミラーゼの由来によっ
てpH、温度の影響が大きく、又経済面等において満足し
得るものではない。2. Description of the Related Art Most enzyme agents used at present are powder products, which are inconvenient to handle, such as dissolution at the time of use as compared with an enzyme aqueous solution, and the problem of dust remains. Among them, the biggest problem at the time of use is that, due to the rapid progress of mechanization in recent years, it has become impossible to respond in powder form, and in the industry where a large amount of enzymes are used, there is a demand for an aqueous solution that can be easily incorporated into a work line. However, although the enzyme product is stable in a powder state, it is very unstable in an aqueous solution state and has poor storage stability.
In particular, when the temperature of the amylase aqueous solution increases, the amylase activity easily decreases rapidly and becomes inactive. Moreover, if the aqueous solution itself is left for a long time, there is a problem that it rots and cannot be used. Conventionally, as stable storage of enzyme aqueous solution,
Salts, alcohols and the like are added, but they do not improve the stabilization of enzyme activity even though they have an antiseptic effect (see JP-B-63-17690). In addition, various stabilizers have been studied, but the influence of pH and temperature is large depending on the origin of the amylase, and it is not satisfactory in terms of economy and the like.
発明が解決しようとする問題点 本発明の目的は、従来学問上水溶液の状態で長時間の
安定化することは、不可能に近いとい言われていた。そ
の技術的欠点を解消するため、数多くの実験でアミラー
ゼの失活を止める、優れた安定化剤を発見した。その結
果、輸送、貯蔵中においても長時間安定で、尚且つ効果
を維持し得る、アミラーゼ水溶液製品を提供する。Problems to be Solved by the Invention The object of the present invention has been said to be almost impossible to stabilize in the state of an aqueous solution for a long period of time. To overcome its technical drawbacks, numerous experiments have led to the discovery of excellent stabilizers that stop the deactivation of amylase. As a result, the present invention provides an aqueous amylase product which is stable for a long time during transportation and storage and can maintain its effect.
問題点を解決するための手段 本発明は、上記目的を達成するためにグリシン、リゾ
チーム、プロピレングリコールを配合添加することによ
る、酵素水溶液中のアミラーゼ活性の安定化方法を提供
することである。Means for Solving the Problems The present invention provides a method for stabilizing amylase activity in an aqueous enzyme solution by adding glycine, lysozyme, and propylene glycol to achieve the above object.
作用 本発明の酵素水溶液は、グリシン、リゾチーム、プロ
ピレングリコールを添加することにより、アミラーゼ活
性の保存安定性が顕著に向上される。事実、後記実施例
に示す通り、バチルス・ズブチリス由来アミラーゼ水溶
液にグリシン、リゾチーム、プロピレングリコールを添
加、さらに食塩を添加し、40℃で３ケ月放置後の残存活
性は高力価（10,000単位）、低力価（低力価は高力価の
1/100）共に70〜90％を保持している。無添加の場合は
高力価で残存活性が30以下％で、低力価では１ケ月間で
20％以下、３ケ月間では完全に失活してしまう。故に本
発明の酵素水溶液は非常に優れた保存安定性を具備して
いる。本発明者の研究によれば、酵素水溶液中のアミラ
ーゼ活性の安定化効果は、グリシン、リゾチーム、プロ
ピレングリコールの３物質の相乗効果によるもので、後
記実施例で示すとおり、３物質の内１つでも欠けると、
著しく不安定になる。尚、本発明の酵素水溶液は、優れ
た安定性を有する為、夏季の気候条件下においても通常
の保存状態で十分長期保存が可能で、製造当初のアミラ
ーゼ活性を持続し得る。Action The storage stability of amylase activity is remarkably improved by adding glycine, lysozyme and propylene glycol to the aqueous enzyme solution of the present invention. In fact, as shown in Examples below, glycine, lysozyme, and propylene glycol were added to an aqueous solution of Bacillus subtilis-derived amylase, and then sodium chloride was added. Low titer (low titer means higher titer
1/100) both hold 70-90%. In the case of no addition, the residual activity is 30% or less at high titer, and within 1 month at low titer.
Under 20%, it will be completely deactivated in three months. Therefore, the enzyme aqueous solution of the present invention has very excellent storage stability. According to the study of the present inventor, the stabilizing effect of the amylase activity in the enzyme aqueous solution is due to the synergistic effect of three substances of glycine, lysozyme and propylene glycol. But if you lack it,
It becomes extremely unstable. In addition, since the enzyme aqueous solution of the present invention has excellent stability, it can be stored for a sufficiently long period in a normal storage condition even under summer climatic conditions, and can maintain the amylase activity at the beginning of production.
実施例 本発明を詳しく説明するため、実施例を挙げる。Examples Examples will be given to explain the present invention in detail.
実施例Ｉ 酵素、バチルス・ズブチルス由来のアミラーゼを含有
した酵素水溶液（低力価）に、食塩10％を配合し下記実
施処方例（第１表）に従い水で調製した水溶液を、40
℃、３ケ月間の経時変化、即ち、残存アミラーゼ活性
（％）、水溶液の外観を比較観察した。その結果は下記
第２表、第３表の通りで、本発明の酵素水溶液中のアミ
ラーゼ活性の安定化を得た。尚、対照として、グリシ
ン、リゾチーム、プロピレングリコール無添加の酵素水
溶液についての結果も併記した。Example I An aqueous solution prepared by mixing 10% of sodium chloride with an aqueous solution of an enzyme (amylase derived from Bacillus subtilis) (low titer) according to the following example (Table 1) was prepared.
At 3 ° C., changes over time for three months, that is, the remaining amylase activity (%) and the appearance of the aqueous solution were compared and observed. The results are shown in Tables 2 and 3 below, stabilizing the amylase activity in the enzyme aqueous solution of the present invention. As a control, the results for an enzyme aqueous solution without glycine, lysozyme and propylene glycol are also shown.
尚、３ヶ月以後の40℃保存の残存活性については、実
施処方例２は７ヶ月目でも42％の活性があった。 Regarding the residual activity after storage for 3 months at 40 ° C., Example Formulation Example 2 had an activity of 42% even at the 7th month.
上記の結果により、実施処方例２即ち、３物質を配合
することにより、本発明の酵素水溶液中のアミラーゼ活
性の安定化を得た。From the above results, stabilization of amylase activity in the enzyme aqueous solution of the present invention was obtained by blending Example Formulation Example 2, ie, three substances.
実施例II 実施例Ｉにおいて、実施処方例２の内で、プロピレン
グリコールの配合量を10％、15％にした場合の残存アミ
ラーゼ活性（％）を同条件にて安定性試験を行った結果
は、第４表の通りで本発明の酵素水溶液中のアミラーゼ
活性の安定化を得た。Example II In Example I, the stability test was performed on the residual amylase activity (%) when the blending amount of propylene glycol was changed to 10% and 15% in Example 2 under the same conditions. As shown in Table 4, the amylase activity in the aqueous enzyme solution of the present invention was stabilized.
Claims (1)
グリコールの３物質水溶液濃度を4.0重量％〜飽和水溶
液濃度を含有させたことを特徴とする酵素水溶液中のア
ミラーゼ活性の安定化方法。1. A method for stabilizing amylase activity in an aqueous enzyme solution, wherein the aqueous solution of glycine, lysozyme and propylene glycol has an aqueous solution concentration of 4.0% by weight to a saturated aqueous solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14878690A JP2936340B2 (en) | 1990-06-08 | 1990-06-08 | Stabilization of enzyme aqueous solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14878690A JP2936340B2 (en) | 1990-06-08 | 1990-06-08 | Stabilization of enzyme aqueous solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0440893A JPH0440893A (en) | 1992-02-12 |
| JP2936340B2 true JP2936340B2 (en) | 1999-08-23 |
Family
ID=15460652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14878690A Expired - Fee Related JP2936340B2 (en) | 1990-06-08 | 1990-06-08 | Stabilization of enzyme aqueous solution |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2936340B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR28788A (en) * | 1993-05-25 | 1997-03-25 | Henkel Ecolab Gmbh & Co Ohg | Methods and equipment for machine-dishwashing cleaning. |
| JP4733861B2 (en) * | 2001-06-20 | 2011-07-27 | 花王株式会社 | Enzyme particles |
| JP4643212B2 (en) * | 2003-11-28 | 2011-03-02 | シスメックス株式会社 | Cholesterol dehydrogenase stabilization method, cholesterol dehydrogenase-containing composition, and cholesterol measurement reagent |
| KR20100061441A (en) * | 2007-07-06 | 2010-06-07 | 래크리드 인코포레이티드 | Use of hydrolytic and oxidative enzymes to dissolve biofilm in airway passages |
-
1990
- 1990-06-08 JP JP14878690A patent/JP2936340B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0440893A (en) | 1992-02-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |