JP2929571B2 - Composting of perishable waste - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention mainly relates to a method for artificially treating putrefactive waste consisting of livestock excrement such as chicken dung and cow dung to compost it in a short time.

[0002]

2. Description of the Related Art In treating spoilage waste mainly composed of excrement of livestock such as chickens, pigs, cows, and horses, generally,
This is fermented into compost or used as landfill material, but in the case of landfill, it is becoming difficult to secure a disposal site, and most of the landfills do not rely on composting technology. Is getting worse.

[0003] A general technique of composting is a natural fermentation method, in which the excrement is accumulated in a pit or the like, and air is forcibly fed from below or cut off as necessary to ferment.

[0004] In this case, however, fermentation at anaerobic and low temperature is apt to occur even though air is fed or cut back, resulting in generation of offensive odor.

[0005] For this reason, fermentation usually takes 1 to 12 months because of natural fermentation. In particular, when rice husks or wood chips are mixed, these fermentations are difficult to ferment and remain, so that abnormal fermentation occurs after fertilization.
In addition, there is a problem that it is difficult to perform uniform composting due to uneven fermentation.

For this reason, recently, many techniques for adding various microorganisms to the spoilage waste to promote fermentation and the like have been disclosed. As the microorganism to be added, a mixture of microorganisms isolated from forest rot, or a single kind of bacteria such as koji mold and photosynthetic bacteria are known. Examples of the method of addition include a method of directly adding a microorganism culture solution to a raw material and a method of attaching a microorganism to an appropriate carrier and mixing the raw material.

However, since these microorganisms are all mesophilic bacteria, there is a disadvantage that the form of the microorganism is extremely strongly inhibited in any of the material forms after the heat of fermentation.

[0008] Further, since microorganisms which are not originally present in spoilage waste are cultured and used, the cost is high.

[0009]

SUMMARY OF THE INVENTION In view of the above-mentioned problems of the prior art, the present invention can minimize the generation of offensive odor during the fermentation process, and can greatly reduce the time required for fermentation. It aims to provide a method for composting the putrefactive waste obtained.

Another object of the present invention is to provide a method for composting putrefactive waste which can obtain a homogeneous composting material at a relatively low cost without adding microorganisms from the outside.

[0011]

Means for Solving the Problems In order to achieve the above object, the method of the present invention is characterized in that spoilage waste is matured by Bacillus SP Gallus X (hereinafter referred to as Gallus X).

[0012] Gallus X is a fungus present in spoilage wastes, and is isolated and examined for mycological properties as follows. (1) Morphology The cells are rod-shaped, bacteria having a minor axis of 1-2 μm and a major axis of 5-7 μm. This bacterium moves actively until about the middle of the logarithmic growth phase, but its motility slows down from about the late logarithmic growth phase, and rarely has motility in the stationary phase. The fungus forms spores. Gram staining is indeterminate.

(2) Growth state (55 ° C.) Gravy agar plate culture IFO Gallus X 12550 12983 13737 Growth good or bad defective defective color Gray White Yellow White Transparent Yellow White Transparent Yellow White Transparent IFO Gallus X 12550 12983 13737 Glossy No Yes Yes Diffusible dye No No No No No Gravy agar slant culture Growth Good Poor Poor Poor Poor IFO Gallus X 12550 12983 13737 Color Gray White Yellow white transparent Yellow white transparent Yellow white transparent Luster No Yes Yes Yes Diffusible dye No None None None Juice liquid Culture Surface development Phosphofilm formation Phosphofilm formation Phosphofilm formation Presence or absence of pellicle formation None None None None Litmus milk pH No change No change No change No change Coagulation----Liquefaction----

(3) Physiological properties Reduction of nitrate----Vaginal reaction----MR test----VP test----Indole formation----Formation of hydrogen sulfide----Starch Hydrolysis of +++++ Utilization of citric acid Coser----Christensen----Utilization of inorganic nitrogen source Utilization of nitrate----Utilization of ammonium salt----IFO Gallus X 12550 12983 13737 〓 Production----〓 Urease----〓 Oxidase + ± ++ 〓 Catalase----の Growth range pH4----5----6 ++++++++++++ 7 ++++++++++++++ 8 ++++++ ++ ++ 9 ± ± ± ± 10----Temperature 37 ° C----45 ++ ++ ++ 50 ++ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 65

(4) Use of carbon compound and generation of acid and gas L-arabinose---D-xylose----D-glucose----D-mannose----D-fructose-- --D-galactose-----IFO Gallus X 12550 12983 13737 Maltose-----Sucrose----Lactose----〓 Trehalose----〓 D-Sorbit----〓 D-Mannit − − − − 〓 Inosit − − − − 〓 Glycerin − − − − 〓 Starch − − − − ++++ Growing very well ++ Growing well + Growing ± Growing very slightly − Not growing

(5) Determination of GC content Bacillus stearotherophilus (Bacillus stearo) whose growth temperature range is similar to that of Gallus X bacterium
thermophilus) GC with IFO 12550
The contents were compared.

1. Bacterial strains used: Gallus X, Bacillus stearothermophilus (Bac
illus stearothermophilus)
IFO 12550 was used.

2. Culture conditions Each strain was inoculated into a liquid medium obtained by adding 0.1% glucose to Difco's Heart Infusion Broth.
The culture was stirred overnight at 0 ° C and 65 ° C. 50 ° C for each strain
The growth condition at 65 ° C. and at 65 ° C. was checked, and the culture broth with good growth was used as a seed. 10% of each seed strain was added to a fresh medium, and cultured for 5 hours at a temperature at which growth was good, to obtain a sample for measuring GC content.

3. DNA extraction A 10 ml sample was centrifuged to collect bacteria, and then salin was collected.
After suspending well in e-EDTA, 10,000 xg10
Centrifuged again for minutes and discarded the supernatant. The cells were rapidly frozen with methanol / dry ice. Lysozyme 2mg / ml-10mM Tris-H
0.5 ml of Cl (pH 8.0),
The reaction was performed for minutes to 2 hours. 50 μl of Tris-SDS buffer was added, mixed well, and heated at 60 ° C. for 5 minutes. Add 0.2 ml of 90% (v / v) phenol,
Shake vigorously for 2 minutes. Cool with ice water, add 0.2 ml of chloroform and add
Shake vigorously for a minute. Centrifuged at 10,000 × g for 5 minutes. The solution in the upper layer was added to prevent the precipitation of the intermediate layer from rolling up.
4 ml was gently sucked and transferred to another polypropylene tube. Add 0.5 ml of chloroform and shake for 2 minutes,
Centrifuged at 10,000 × g for 5 minutes. ０．３ 0.3 ml of the solution in the upper layer was sucked so as not to wind up the precipitate in the intermediate layer, and transferred to another polypropylene tube. 〓 and 〓 were repeated once. ５０ 50 μl of an RNase solution was added and reacted at 37 ° C. for 10 minutes.加 え Add 50 μl of proteinase K solution, and add
The reaction was performed for 0 minutes. ９０ 0.2 ml of 90% phenol, 0.2 chloroform
ml was added and shaken for 1 minute.遠 心 Centrifuge at 10,000xg for 5 minutes, and supernatant 0.3ml
Transferred to another polypropylene tube. ０．７ 0.7 ml of 99% ethanol was added and shaken for 1 minute. ＤＮＡ The precipitated DNA was rinsed with 70% ethanol and then with 99% ethanol.乾燥 Dry with a vacuum desiccator.

4. Preparation of GC content measurement sample To the sample of the above ①, add 50 μl of sterile distilled water,
For 1 hour. Then, it was rapidly cooled after heating at 100 ° C. for 5 minutes. 10 μl aliquots were placed in polypropylene tubes. The nuclease P1 solution was added in an amount of 10 μl each, and after closing the lid, the mixture was gently flicked with a finger and centrifuged for several seconds. The reaction was performed at 50 ° C. for 1 hour. Alkaline phosphatase solution was added in an amount of 10 μl each, and after closing the lid, the mixture was gently flicked with a finger and centrifuged for several seconds. The reaction was performed at 37 ° C. for 1 hour. This solution was directly used as a sample for HPLC.

5. Operating conditions of HPLC Column: L-colu manufactured by Chemical Inspection Association
mn ODS Eluent: 0.2 M NH4H2PO4-acetonitrile = 20: 1 Flow rate: 0.5 ml / min Detector: UV spectrophotometer Detection wavelength: 260 nm Temperature: room temperature

6. Calculation of GC content The calculation of the GC content was in accordance with the following equation. Cx (Gx, Tx, Ax) is a nuclease P1 digested D
Indicates the dCMP (dGMP, dTMP, dAMP) peak area of NA. FIG. 2 shows the base composition ratio of Galus X by HPLC. 5.41, 5.70, 6.1
8 and 6.56, respectively, dCMP, dGMP, dTM
P and dAMP peaks were observed. FIG.
The base composition ratio of 12550 is shown. 5.37,5.
DCMP and dG at 71, 6.19 and 6.57, respectively.
Peaks of MP, dTMP and dAMP were observed. Based on these peaks, the calculated Galus X and IFO 125
The GC content of 50 is as follows: Bacterial GC content (mol%) Gallus X 31.1 IFO12550 40.9 Gallus X was obtained from Bacillus stearothermophilus (B. s.
tearothermophilus) IFO1255
0 was found to be somewhat similar to Gallus X in the growth temperature range, differing from any of the other IFOs 12983 and 13737. Therefore, IFO12550 and Galus X, which were similar to Galus X in the growth temperature range,
When the GC content was compared with the above, a difference of about 10% was shown.

From the above results, Gallus X was determined to be a new species of the genus Bacillus, and Gallus X (Bacillus sp.
GallusX). This microorganism was sent to the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology by
Deposited as No. 1.

In the present invention, the above-mentioned Gallus X fungus is multiplied and fermentation of putrefactive waste is carried out. The specific method is as follows. That is, the putrefactive waste is pulverized and kneaded into a paste, and the paste-like material to be processed is heated in a low oxygen environment by frictional heat or heating accompanying the pulverization and kneading. After suppressing the growth of or killing microorganisms containing malodorous bacterium-producing bacteria such as facultative anaerobic bacteria that survive in the material to be treated, while maintaining the temperature of the putrefactive waste at a high temperature, under aerobic conditions The fermentation is started by preferentially starting the fermentation by the Galus X bacteria surviving under the high temperature and oxygen-deficient environment to obtain a composting material.

The raw materials to be treated by the method of the present invention include not only livestock dung but also all putrefactive organic wastes such as excess sludge of water and sewage and food and drink residues.

In order to pulverize and knead the putrefactive waste into a slurry, for example, a processing cylinder having an inlet at one end and an outlet at the other end, and having a pressure screw member and pulverizing teeth therein. Is used. This processing cylinder is fixed horizontally, and when the putrefactive waste filled from the inlet is pumped toward the outlet, the putrefactive waste is finely pulverized by the cooperation of the screw member and the pulverizing teeth. They are kneaded at the same time. The inside of the processing cylinder is filled with the putrefactive waste without gaps. For this reason, the putrefactive waste is kneaded in the above-mentioned pumping process in a state where contact with air is remarkably suppressed, that is, in a poor oxygen state, and generates high frictional heat.

It is desirable to adjust the water content of the putrefactive waste to 50 to 65% before being put into the processing tube. For moisture control, dried livestock dung and the like may be added to the putrefactive waste, but it is desirable to add a fermentation product produced by the method of the present invention. Further, the temperature at which the temperature is increased as the object to be treated is 5 in order to preferentially grow Galus X bacteria.
Optimally between 0 ° C and 60 ° C, but between 45 ° C and 70 ° C
It is sufficient if it is within the range. The temperature may be adjusted by changing the amount of frictional heat generated by adjusting the rotation speed of the screw member provided in the processing chamber, or an external heating method using a heater or the like may be employed.

The object discharged from the processing tube is aerobicly crushed, for example, while maintaining the high temperature state, whereby preferential growth of Galus X bacteria is achieved. This Galus X bacterium may not only survive in a high temperature and oxygen-deficient environment, but may also be added externally as a post-treatment. The externally added Galus X bacterium may be in any state as long as it is a viable cell, and examples of the form of use include a culture solution, a viable cell suspension, a viable cell solid, and a dried product. The amount added is not particularly limited.

[0029]

According to the method of the present invention, the galus X bacteria are mixed in the putrefactive waste in advance, or the putrefactive waste originally containing the gallus X bacteria is ground and kneaded in a non-contact state with air. Paste. During the process of forming the paste or afterwards, the temperature of the material to be treated is increased in anoxic or extremely oxygen-deficient condition, so that microorganisms such as aerobic bacteria and facultative anaerobic bacteria present in the putrefactive waste are removed. An environment that cannot grow is formed.

An object to be treated which has been sufficiently pulverized and kneaded in an oxygen-free or extremely oxygen-deficient state until the components in the raw material are uniform is finely pulverized under aerobic conditions while maintaining a high temperature state. Therefore, in the fermentation process under aerobic conditions, the growth of microorganisms such as facultative anaerobic bacteria involved in the production of malodorous substances remains inhibited. On the other hand, when air is supplied to the high-temperature processing target, the growth of Galus X bacteria, which is an aerobic thermophilic bacterium that has been suppressed until then, is started, and rapid fermentation is performed.

In addition, coarse organic substances mixed in the object to be treated are finely divided by pulverization and kneading, and this organic substance is uniformly distributed in the object to be treated while containing an appropriate amount of water.
In the fermentation process, microbial decomposition is efficiently and uniformly performed over the whole of the material to be treated, and uneven fermentation is prevented.

[0032]

EXAMPLES Hereinafter, the present invention will be described more specifically based on examples. Example 1 1000 kg of cow dung with a water content of about 75% and a water content of about 20
% Of cow manure compost 800 Kg was put into the first treatment cylinder and mixed to make the water content about 56%. Then, while gradually moving toward the discharge port by the rotating blades, the pressure was increased by pushing back by the opposing blades. While crushing and kneading. The putrefactive waste rapidly rises in temperature due to the mutual frictional heat accompanying the kneading and becomes a paste, filling the inside of the treatment tank without gaps.

The workpiece discharged from the first processing cylinder is transferred to the second processing cylinder.
The fermentation was started by continuously introducing the mixture into a processing cylinder, where the mixture was uniformly ground and contacted with air while maintaining a high temperature state. After the fermentation was started, the material to be processed was taken out of the second processing cylinder, and the temperature was measured after 4 to 5 hours.
The product temperature was about 70 ° C. After that, turning over was performed every day for 4 days to obtain a composting material.

Table 1 shows the results of component analysis of the composting material.

[Table 1]

The average value of cow dung compost is 2.3% for total nitrogen, 4.9% for phosphorus, 0.4% for potassium, and 14.1% for carbon. In many cases, the carbon ratio tends to be high.

The cow manure compost requires a high level of fertilizer content as compared with compost, so care must be taken when applying it to ordinary crops, especially fruit crops. That point has been improved and is easier to apply.

Next, the characteristics of the microorganisms separated from the putrefactive waste and the composting material will be described in detail.

Experimental Materials and Methods Preparation of Samples 0.1 g of each requested sample was weighed into each test tube, and 1 ml of physiological saline was added. After vigorously stirring each test tube for 5 minutes, it was allowed to stand for 15 minutes, and the supernatant was used as a sample stock solution. 2. Measurement of viable cell count It was performed by the plate dilution method. The medium used was an ordinary agar medium for separating general bacteria, and a potato dextrose agar medium was used for separating filamentous fungi and yeast. 3. Attitude to Oxygen The aerobic bacteria or facultative anaerobic bacteria were identified by adding an aneropack manufactured by Mitsubishi Gas Chemical Co., Ltd. to a BBL gas pack jar at the time of measuring the viable cell count. Isolates were determined by the OF test. 4. The growth temperature is measured by Sanyo Incubator MR
152. 5. Intestinal bacteria were determined as facultative anaerobic bacilli that show oxidase negative.

[0039]

[Table 2] Table 2 shows the results of the number of microorganisms living in the putrefactive waste and the composting material measured by a plate dilution method using a normal agar medium and a potato dextrose agar medium.

The number of bacteria of each material that can grow at 28 ° C. is 1.0 × 10 9 cells / g sample and 8.8 × 10 8 cells / g sample in the putrefactive waste and the composting material, respectively. After the input of waste, a tendency to decrease was observed. This is because microorganisms multiply by assimilating organic matter in spoilage waste, but as the consumption of organic matter progresses to a certain extent, the amount of organic matter in the material that can keep all the microorganisms that have grown so far is alive. , Which suggests that some microorganisms will starve.
Since the starved microorganisms feed on other living microorganisms, the decrease in the number of microorganisms stops at a certain point and the state is maintained. This is a phenomenon generally observed in compost, soil, and the like, and suggests that the composting material in the present invention has been composted to a state of near maturity.

The breakdown of the microorganisms growing at 28 ° C. is as follows:
All materials were approximately 100% bacterial, but in terms of type, considerable differences were found between putrefactive waste and composting material. Gram-negative bacteria, including red pigment bacteria that appear to be photosynthetic bacteria belonging to the genus Rhodoshudomonas, are the overwhelming priority species in putrefactive waste, and only a very small number of gram-positive bacteria belonging to the genus Bacillus are observed. No (see Table 3). In addition, the number of facultative anaerobic bacteria was 5.0 × 10 5 cells / g sample, which accounted for only 0.2% of the total number of isolates in the putrefactive waste, and it was found that most of them were aerobic bacteria ( Table 3). On the other hand, in the case of the composting material, the appearance of the colony was similar to that of the material to be treated, but the number of facultative anaerobic bacteria was reduced to about 1/10 (Table 3). This is probably due to the heat of fermentation. The colony properties of facultative anaerobic bacteria were the same for all materials, and were small white colonies and pinpoint colonies.

[Table 3]

The microorganisms grown at 55 ° C. were mainly bacteria, as in the case of the culture at 28 ° C. The microbial count of each material was 10 6 cells / g sample in spoilage waste, but increased to 10 8 cells / g sample in composting material (Table 3). That is, the number of bacteria having a growth temperature range of 55 ° C. (thermophilic bacteria or thermotolerant bacteria) is the most commonly used bacteria having a growth temperature range of 28 ° C. (mesophilic bacteria: natural environment) even under the highest conditions. The number of detected bacterial groups and those belonging to the Enterobacteriaceae family that produces foul odors is also small (approximately 1/10), suggesting that the fermentation as a whole is proceeding mainly with mesophilic bacteria. However, it was found that the ratio of the bacteria having a growth temperature range at 55 ° C. among the respective materials increased about 100 times as much as that of the composted material (Table 3).

Thus, in the fermentation process of the present material, it is possible that thermophilic bacteria or thermotolerant bacteria are involved in the generation of heat of fermentation and the fermentation during the period of generation of heat of fermentation. Sex was strongly suggested.

Regarding the generation of odor, the putrefactive waste had a strong fecal smell, but the composting material disappeared with only a faint earthy smell, indicating that the quality after fermentation was maintained. Was.

As described above, the microorganisms contained in each material were overwhelmingly bacterial in all the materials, and no filamentous fungi or yeast were detected.

Further, when a growth temperature range test of the bacteria isolated so far was performed, bacteria that did not grow at 37 ° C. but grew well at 55 ° C. were isolated. This bacterium is an aerobic bacterium of undefined gram and has a spore-forming ability.
It was suggested that it may be a strain of Stearothermophilus (Table 4). Therefore, Bacillus stearothermophilus IFO 12550, 12983, 1
When 3 strains of 3737 were ordered and compared, IFO12
Although 550 was slightly similar to Gallus X in the growth temperature range, the other two strains showed significant differences in growth temperature range, motility, and colony properties from Galus X.

In the present fermentation process, the faecal odor disappears because the growth of facultative anaerobic bacteria including intestinal bacteria is suppressed while the heat of fermentation is generated, while the thermophilic Bacillus genus contained in feces is contained. This is probably because the fermentation was continued by the bacteria Gallus X.

Example 2 1000 kg of cow dung with a water content of about 75% and a water content of about 14
% Of rice hulls was treated in a galus plant in the same manner as in Example 1, and then fermentation was started. 4 ~ from fermentation start
After a lapse of 5 hours, the temperature of the product reached about 70 ° C. due to the heat of fermentation.
Thereafter, cutting was performed every day for one week to 10 days to obtain a composting material.

Example 3 1000 kg of chicken manure with a water content of about 75% and a water content of about 14
% Of rice hulls was treated in a galus plant in the same manner as in Example 1, and then contacted with air while maintaining a high temperature state to start fermentation. After 4 to 5 hours from the start of fermentation, the temperature of the product reached about 65 ° C. due to the heat of fermentation. Then 7
For 2 days, cutting was performed every second day to obtain a composting material.

Example 4 A scallop having a water content of about 80% and a wheat stalk having a water content of about 20% were mixed in a weight ratio of 6 to 4 and treated in a galls plant in the same manner as in Example 1; While maintaining the state, it was put into a box with a hole and fermentation was started. After 4 to 5 hours from the start of fermentation, the temperature of the product reached about 65 ° C due to the heat of fermentation. After that, for 7 days, we piled up without switching back,
Composting material was obtained.

Example 5 Straw having a moisture content of about 60% used as a litter in a stall was treated in a galus plant in the same manner as in Example 1, and then contacted with air while maintaining a high temperature to start fermentation. . After a lapse of 4 to 5 hours from the start of fermentation, the temperature of the product is about 65
° C. After that, cutting was performed every second day for 5 days to obtain a composting material.

Example 6 1000 kg of pig dung with a water content of about 85% and a water content of about 14
% Of rice hulls was treated in a galus plant in the same manner as in Example 1, and then put into a perforated box while maintaining the high temperature, and air was ventilated from below to start fermentation. After 4 to 5 hours from the start of fermentation, the temperature of the product reached about 65 ° C. due to the heat of fermentation, and composted in 7 days.

An example in which the material composted by the method of the present invention is used for soybean cultivation will be described below. 1. Test method (1) Test crop soybean (Fukuyutaka) (2) Test period July-November 1990 (3) Test plot The fertilizer test was conducted in 9 test plots. Table 5
Shown in Test Zones 1 to 3 are zones to which a material obtained by composting cow dung by the method of the present invention is applied, and Test Zone 1 is a zone where 10
The test zone 2 which applied 250 kg per a, the test zone 2 was the main composting material 500 kg, and the test zone 3 was the main composting material 500 kg.
It is a ward where 50 kg of charcoal has been applied to the plant. The carbon / nitrogen ratio (C / N) of the composting material used in this test was 17, and the contents of nitrogen (N), phosphoric acid (P), and potassium (K) were 1.09, respectively. 0.32, 1..
38%. Test plots 4 to 6 are plots to which the material obtained by composting pig manure by the method of the present invention is applied, and test plot 4 is a plot to which the above-mentioned pig manure composting material is applied at 250 kg per 10 g, and a test plot 5
Is the above-mentioned pig manure composting material of 500 kg, and the test zone 6 is a zone in which the above-mentioned pig manure composting material of 500 kg is added with 50 kg of charcoal. The nitrogen (N), phosphoric acid (P), and potassium (K) contents of the pig manure composting material used in this test were 1.30 and 0.6, respectively.
2, 0.48%. Test plots 7 to 8 are plots to which a compost produced by conventional technology for cow dung was applied, test plot 7 was a plot to which 250 kg of this composting material was applied per 10 g, and test plot 8 was to apply the same composting material of 500 kg. It is a ward.
The carbon / nitrogen ratio (C / N) of the above materials used in this test was 15.5, and the contents of nitrogen (N), phosphoric acid (P), and potassium (K) were 0.45, respectively. 0.65,
0. 65%. Test zone 9 is a compost-free zone.
Only basal fertilizer is applied.

[Table 5]

(4) Outline of Cultivation Type Cultivation leveling: 7/6 tractor rotary with depth 10
Seeding: 4.8 plants per 7/7 m2 (75 cm x
28cm) 1 point 3 grain seeding Application: 7 / 7m2 Base fertilizer PK Chemical (P20%, K2
0%) 30Kg / 10a Medium cultivation soil: 1st 7/20, 2nd 8/8 control machine Pest control: 1st 8/20 Ministop M dust 4
Kg / 10a, 2nd 8/31 Badan powder 4Kg / 10
a, 3rd 9/15 lanthanate wettable powder 1,000 times liquid 4th 10/1 lannate wettable powder 1,000 times liquid 5th 10/15 lannate wettable powder 1,000 times liquid

2. Table 6 shows the results of the test and the results of the test. This Table 6
The following can be said from (1) The material obtained by composting cow dung and swine dung by the method of the present invention generally had better growth and higher yield than non-application. (2) With respect to the application rate, 500 Kg of a material obtained by composting cow dung by the method of the present invention, and 250 kg by a material obtained by composting pig dung by the method of the present invention, showed a good yield. (3) A section where 500 kg of material composted by the method of the present invention using cow dung or pig dung as a raw material is applied, and charcoal is added,
It was recognized that the fertilization was improved by the addition and the sales increased. (4) From the above, it was confirmed that, for the material composted by the method of the present invention using cow dung as a raw material, the yield increased with the application of 500 kg, but further increased with the use of charcoal. With the material composted by the method of the present invention using pig dung as a raw material, the application of 500 kg grew well, but the ripening was poor and the yield decreased. However, it was recognized that the use of charcoal could prevent this and increase the sales.

[Table 6]

[0056]

As described above, according to the present invention, since the Galus X bacteria existing in the putrefactive waste are grown to compost the putrefactive waste, it is extremely economical and controllable. The perishable waste can be composted in an easy manner.

Further, according to the present invention, Galus X bacteria are preferentially proliferated to putrefactive waste to promote fermentation, so that facultative anaerobic bacteria in putrefactive waste can be killed or eliminated. Since growth is suppressed, composting can be performed without generating bad odor.

Further, in the present invention, if the spoilage waste is pulverized and kneaded and the temperature is raised in an oxygen-deficient environment to promote fermentation by Galus X bacteria, a uniform composting material without uneven fermentation can be obtained. What you can get.

[Brief description of the drawings]

FIG. 1 is a flow sheet of a method for treating putrefactive waste according to the present invention.

FIG. 2 Base composition ratio of Galus X by HPLC analysis

FIG. 3. B. by HPLC. stearothermo
base composition ratio of philus IFO12550

[Table 4]

Claims (5)

(57) [Claims] 1. A method for composting putrefactive waste, which comprises maturing putrefactive waste with Bacillus SP Gallus X. 2. A method in which putrefactive waste is pulverized and kneaded into a paste, and the paste-like material is heated in a low oxygen environment by frictional heat or heating accompanying the pulverization and kneading. , Once suppressing the growth of microorganisms in the object to be treated, and then maintaining the temperature of the object to be treated at a high temperature,
Contacting the object with air to initiate preferential growth of Bacillus SP Gallus X bacteria in the surviving object under the high temperature and oxygen-deficient environment; Law. 3. The method for composting putrefactive waste according to claim 2, wherein Bacillus SP Gallus X is added to the putrefactive waste or the outside of the object before contacting the object with air. A method for composting putrefactive waste, characterized in that: 4. The method for composting putrefactive waste according to claim 2, wherein the temperature of the putrefactive waste is maintained at 40 ° C. to 70 ° C. 5. The method for composting putrefactive waste according to claim 2, wherein the moisture of the putrefactive waste is adjusted to 50 to 65%.