JP2926173B2 - Mono and oligo galacturonic acid production equipment - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an apparatus for automatically producing mono- and oligogalacturonic acid from pectin and pectic acid powder.

[0002]

2. Description of the Related Art In recent years, mono- and oligogalacturonic acids have been attracting attention because they have been suggested to be involved in the bacteriostatic action of Escherichia coli and the like, and to increase the yield of agricultural crops due to the plant's defense against microorganisms. ing. Heretofore, for example, mono- and oligogalacturonic acids have been produced by reacting pectin or pectic acid with enzymes derived from various microorganisms in a badge system. However, in general, the amount of enzyme secreted by microorganisms is small, and in the badge type, it is disposable each time an enzyme reaction is performed, and the consumption of the enzyme amount increases as the production amount of mono- and oligogalacturonic acid increases, which is inefficient. . In addition, as the enzyme reaction is performed in a larger amount, the reaction conditions tend to be uneven, and the produced mono- and oligogalacturonic acids also tend to be uneven. For this reason, there is a disadvantage that complicated operations are not suitable for mass production.

[0003] The applicants of the present application have previously proposed a manufacturing method that eliminates these disadvantages (Japanese Patent Laid-Open No. 6-20568).
No. 7 published). This method is a method for producing mono- and galacturonic acid by feeding a pectin or pectic acid solution to a water-insoluble immobilized carrier on which an enzyme is immobilized, and is an efficient method.

[0004]

SUMMARY OF THE INVENTION Incidentally, in the method for producing mono- and oligogalacturonic acids according to the above-mentioned proposal, a pretreatment step for producing pectin or pectic acid solution from pectin or pectic acid powder, a pectic acid solution And the subsequent post-treatment process must be performed manually one by one, which has been a problem in that the work is complicated and takes a long time, resulting in poor production efficiency.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned problems, and enables pre-processing, reaction, and post-processing to be performed automatically, thereby improving production efficiency and achieving mass production. An object of the present invention is to provide a suitable apparatus for producing mono- and oligogalacturonic acid. Then, the point is to improve the function of each section as necessary.

[0006]

In order to achieve the above object, an apparatus for producing mono- and oligogalacturonic acid using pectin or pectic acid powder as a raw material according to the present invention comprises a raw material supply means for supplying a predetermined amount of the raw material. A raw material solution generating means for adding a buffer solution to the raw material supplied from the raw material supply means to generate a predetermined amount of the raw material solution, a filtering means for filtering the raw material solution generated by the raw material solution generating means, An enzymatic reaction means for performing an enzymatic reaction with a water-insoluble immobilized carrier in which a decomposing enzyme is bound to the raw material solution filtered by the filtration means; And desalination treatment means for performing the following.

[0007] And, the upper Symbol raw material supply means, a hopper for storing the raw material, a guide tube for guiding the raw material supplied from the outlet opening of the hopper, the rotary shaft Toshikatsu guide tube the axis of the guide tube And a transfer rod in the form of a male screw for transferring the supplied material toward the discharge port of the guide tube by this rotation, and a motor for rotating the transfer rod by a predetermined number of rotations.

[0008] was Soshitema, on SL raw material solution generating means, and a raw material tank containing the raw material supplied from the raw material supply means, and the pure water supply unit for supplying pure water to the raw material tank,
A buffer supply unit for supplying a buffer into the raw material tank,
Raw material supplied into the material tank, a stirring portion for stirring the pure water and buffer, and constitutes a heater for the solution in the upper Symbol material tank heating.

[0009] Also, the upper Symbol filtration means, a filtrate tank having an outlet portion which are opened and closed to the inlet portion and timely raw material solution is placed generated in the raw material solution generating means, a plurality of the raw material solution to pass through A through-hole is provided, a tray is provided with a support surface for supporting the filter paper and is moved and arranged at the inlet of the filtration tank, a stacker for stocking a plurality of trays, and arranged at the inlet of the filtration tank. A tray moving mechanism for retreating the tray from the inlet portion, taking out a new tray from the stacker and moving the tray to the inlet portion, and an aspirator for sucking the inside of the filtration tank are provided.

[0010] Further, the upper Symbol enzyme reaction means a reaction column packed with the raw material solution is passed through a water-insoluble immobilized carrier which is degrading enzymes is bound, feeding a raw material solution to the reaction column Kyusuru material solution And a feed pump. Ma
Was, prior position of the upper Symbol reaction column, the raw material solution is filled a small carrier particle sizes than through the feed raw material solution water-insoluble immobilized carrier of the reaction column from the raw solution feed pump and setting only the filter column for filtering. Furthermore ,
Bei the temperature holding portion for holding the upper Symbol reaction column in a constant temperature Etei
You.

Further, the upper Symbol desalting unit, either to a pair of desalting ion exchange resin column, the reaction solution from the enzyme reaction means on one of the ion exchange resin column to allow delivery A switching valve unit for alternately switching the other ion-exchange resin tower so that wash water for washing and regenerating the ion-exchange resin can be supplied, and a wash-water supply unit for supplying wash water for the ion-exchange resin And is configured.

[0012]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an apparatus for producing mono- and oligogalacturonic acids according to an embodiment of the present invention will be described with reference to the accompanying drawings. This manufacturing apparatus is shown in FIGS.
As shown in the figure, an apparatus for producing mono- and oligogalacturonic acid using pectin or pectic acid powder as a raw material. And a pure water generating means 4 to be used. The pure water generating means 4 is configured as shown in FIGS. In the figure, 5 is a well-known pure water device for generating tap water into pure water, 6 is a pure water tank for storing the pure water generated by the pure water device 5, and 7 and 8 are provided before and after the pure water device 5. Filter.

As shown in FIGS. 1 and 2, the manufacturing apparatus includes a raw material supply means 10 for supplying a predetermined amount of raw material,
A raw material solution generating means 30 for adding a buffer to the raw material supplied from the raw material supply means 10 to generate a predetermined amount of the raw material solution;
An enzymatic reaction in which an enzymatic reaction is carried out by a filtering means 50 for filtering the raw material solution generated by the raw material solution generating means 30 and a water-insoluble immobilized carrier having a decomposing enzyme bound to the raw material solution filtered by the filtering means 50 The apparatus includes a means 80, a desalination processing means 100 for performing a desalination treatment of the reaction solution having undergone the enzyme reaction in the enzyme reaction means 80, and a control unit 120 for controlling each means. Hereinafter, each means will be described in detail.

The raw material supply means 10 is configured as shown in FIGS. 2, 4, 5 and 6. In the figure, 11
Is a hopper for storing the raw material, which is formed of a metal, a resin, or the like, and has a substantially frustoconical space or the like, and has a large-diameter portion as an upper supply opening 12 and a small-diameter portion as a lower outlet opening 13. I have. Reference numeral 14 denotes a lid which is detachably provided to the supply opening 12. Reference numeral 15 denotes a guide tube supported by the base 1 to support the hopper 11 and guide the raw material supplied from the outlet opening 13 of the hopper 11, and has a communication port 15a communicating with the outlet opening 13. Reference numeral 16 denotes a discharge port at the tip.
Reference numeral 17 denotes a valve body which opens and closes the discharge port 16 and is moved forward and backward by an air cylinder 18 so as to close the discharge port 16 at the time of advance and open the discharge port 16 at the time of retreat. The air cylinder 18 is attached to the distal end of the guide tube 15 via an attachment member 18a. The air cylinder 18 includes the solenoid valve 1
When the stop signal is received from the control unit 120 via the control unit 9, the vehicle advances, and when the start signal is received, the vehicle moves backward.

Reference numeral 20 denotes a transfer rod, which is formed in the shape of a multi-threaded external thread such as a three-threaded screw, is rotated about the axis of the guide tube 15 as a rotation axis, and is rotated in the guide tube 15 and supplied by the rotation. Discharge material 1 of the guide tube 15
It is transferred to 6. The space formed by the external thread of the transfer rod 20 and the guide tube 15 is appropriately determined, and is determined so that the rotation speed and the discharge amount are proportional. The base end 20 a of the transfer rod 20 is rotatably inserted into and supported by the bearing 21 of the guide tube 15. 22
Is a stepping motor for rotating the transfer rod 20 by a predetermined number of rotations via a motor shaft 22a.
Behind 1, it is fixed to the base 1. The stepping motor 22 drives when there is a start signal from the control unit 120 and stops when there is a stop signal,
The drive time for one operation is set in advance and the number of rotations is set, that is, the discharge amount of the raw material in one operation time is determined.

Reference numeral 23 denotes a stirring section, which includes a vertical rotation shaft 24 extending vertically into the hopper 11, a pair of stirring blades 25 provided at the tip of the vertical rotation shaft 24, and a tip attached to the middle of the vertical rotation shaft 24. Is a stirring rod 2 along the wall of the hopper 11
6 is provided. The vertical rotation axis 24 is the lid 1 of the hopper 11
4 is provided rotatably. The tip 25a of one stirring blade 25 is bent upward along the inner surface of the hopper 11, and the tip 25b of the other stirring blade 25 is
1 is bent downward along the inner surface. 26a is a falling blade provided at the tip of the vertical rotation shaft 24 to face the communication port 15a and drop the raw material of the communication port 15 to the transfer rod 20.

Reference numeral 27 denotes a drive mechanism for the vertical rotation shaft 24,
The rotation of the transfer rod 20 is transmitted to the vertical rotation shaft 24 by a gear mechanism 28 and a belt transmission mechanism 29. The gear mechanism 28 includes a horizontal rotating shaft 28b that is rotatably provided on the lid 14 via a bearing portion 28a and is parallel to the transfer rod 20, and a driving bevel gear 28c that is provided at one end of the horizontal rotating shaft 28b.
And a driven bevel gear 28d provided on the vertical rotation shaft 24 and meshing with the driving bevel gear 28c. The belt transmission mechanism 29 includes a driving pulley 29a provided at the base end portion 20a of the transfer rod 20, a driven pulley 29b provided at the other end of the horizontal rotating shaft 28b, and a belt 29c wound between the pulleys 29a and 29b. Become. Double pulley 29
a, 29b are formed to have the same diameter, and bevel gears 28c, 2
The gear ratio of 8d is 1: 1, and the number of rotations of the transfer rod 20 and the vertical rotation shaft 24 is set to be the same.

The raw material solution generating means 30 is configured as shown in FIGS. 2, 4 and 5. In the figure, reference numeral 31 denotes a raw material tank for storing the raw material supplied from the raw material supply means 10. The raw material tank 31 is supported by the base 1 and has an inlet 32 located below the discharge port 16 of the guide tube 15 and supplied with the raw material. 33 is the entrance 32
And is connected to the valve body 17 via a connecting rod 33a so as to open and close in synchronization with the valve body 17.
An O-ring 33b seals the cap 33 from the periphery of the inlet 32. Reference numeral 34 denotes a pure water supply pump that supplies water from the pure water tank 6 to the raw material tank 31. The raw material tank 31 is provided with a sensor 35 for detecting the liquid level, and the control unit 120 drives the pure water supply pump 34 based on the detection of the sensor 35 to adjust the amount of the raw material solution in the raw material tank 31. You.

Reference numeral 36 denotes a buffer supply unit for supplying a buffer into the raw material tank 31. The buffer supply unit 36 is provided with a sodium acetate solution tank 3 for storing the sodium acetate solution.
7 and the raw material tank 3 from the sodium acetate solution tank 37
1 is provided with a sodium acetate solution supply pump 38 for supplying the sodium acetate solution, an acetic acid solution tank 39 for storing the acetic acid solution, and an acetic acid solution supply pump 40 for supplying the acetic acid solution from the acetic acid solution tank 39 to the raw material tank 31. It is configured. The sodium acetate solution supply pump 38 is driven by the control unit 120 to supply a predetermined amount of sodium acetate solution. Also, the raw material tank 31
Is provided with a pH sensor 41. Based on the detection by the pH sensor 41,
The acetic acid solution supply pump 40 is driven to be H. The sodium acetate solution tank 37 and the acetic acid solution tank 39 are provided with liquid level sensors 42 and 43, respectively, and a warning is issued by the control unit 120 when each solution is insufficient.

Reference numeral 44 denotes an agitator for agitating the raw material, pure water and buffer supplied to the raw material tank 31.
A stirring fan 44b rotated at 4a is provided.
Reference numeral 45 denotes a heater for heating the solution in the raw material tank 31. The raw material tank 31 has a temperature sensor 46 for detecting a temperature.
Is provided, and the temperature sensor 4 is
Based on the detection at 6, the heater 45 is turned on and off so that the temperature of the solution becomes a preset temperature. In FIG. 2, reference numeral 47 denotes a photoelectric sensor attached to a discharge pipe of the raw material tank 31 for detecting emptyness of the raw material tank 31. Numeral 48 is an electromagnetic valve which is opened and closed by the control unit 120 in a timely manner in order to send the raw material solution in the raw material tank 31 to the downstream by natural fall.

The filtering means 50 is configured as shown in FIGS. 2, 7 to 10. In the figure, reference numeral 51 denotes a filtration tank provided with an inlet 52 into which the raw material solution generated by the raw material solution generating means 30 is put and an outlet 53 to be opened and closed at an appropriate time. The filtration tank 51 is fixed to the base 1 via a base member 54. Reference numeral 55 denotes a disk-shaped tray provided with a plurality of through-holes 55 a through which the raw material solution passes and having a support surface for supporting the filter paper 56 and moved to the inlet 52 of the filtration tank 51. Reference numeral 57 denotes a stacker for stacking a plurality of trays 55 and stocking them. The stacker 57 is provided on an upper surface plate 58 provided at the same position as the inlet portion 52 of the filtration tank 51. The stacker 57 presses the side portions of the trays 55 stacked on the upper surface plate 58, and moves the lowermost tray 55 upward. A take-out opening 59 is formed at a lower portion of the lower portion so that the take-out opening 59 can be taken out by sliding contact with the face plate 58.

Reference numeral 60 denotes a tray moving mechanism for retreating the tray 55 disposed at the entrance 52 of the filtration tank 51 from the entrance 52 and taking out a new tray 55 from the stacker 57 and moving it to the entrance 52. The tray moving mechanism 60 is provided so as to be rotatable in contact with the upper surface plate 58 and to be able to pass through the lower extraction opening 59 of the stacker 57,
A hook-shaped engaging member 61 that engages with the tray 55 by rotation to move the tray 55 is provided, and a drive motor 62 provided on the base member 54 and rotates the engaging member 61.

Reference numeral 63 denotes a pressing member which presses and presses the tray 55 positioned at the inlet 52 of the filtration tank 51 into the opening of the inlet 52, and 64 denotes a pressing member provided on the pressing member 63.
An inlet passage of the raw material solution connected to 1, 65 is a pressing body 63
Is an air cylinder that moves up and down, and is supported by a stand 66 provided on the base member 54, and presses the pressing body 63 when it advances, and releases the pressing of the pressing body 63 when it retreats. Numeral 65a is an electromagnetic valve for driving the air cylinder 65. Reference numeral 67 denotes a discharge port through which the tray 55 opened on the upper plate 58 and retreated from the entrance 52 can drop. At the lower part of the discharge port 67, a removable storage container (not shown) for storing the discharged tray 55 is provided.

The stop position of the engaging member 61 is
Is located at the inlet 52 of the filtration tank 51, and the drive motor 62 of the engagement member 61 rotates the engagement member 61 once from this stop position in response to a start / stop signal from the control unit 120. During this process, the tray 55 is
2, is dropped into the discharge port 67, and a new tray 55 is taken out from the stacker 57 and moved and positioned at the entrance 52. The pressing body 63 is released immediately before the driving motor 62 starts, and the driving motor 62
The operation timing of the air cylinder 65 is determined so as to be pressed after the stop of the operation. The drive timing of the drive motor 62 is set before or during the generation of the solution by the raw material solution generation unit 30. 68 in FIG. 2 and FIG.
Is a sensor for detecting the amount of the tray 55;
The warning is issued by the control unit 120 when the number 5 has decreased.

Reference numeral 70 denotes an aspirator for sucking the inside of the filtration tank 51, and is operated by the control unit 120 when the pressing body 63 is pressed and the raw material solution is supplied into the filtration tank 51. In FIG. 2, reference numeral 71 denotes a raw material solution reservoir tank which is provided at the rear of the filtration tank 51 and stores the processing solution in the filtration tank 51, and 72 feeds the filtered raw material solution from the filtration tank 51 to the raw material solution reservoir tank 71. It is a pump. Reference numeral 73 denotes an electromagnetic valve which is provided at the rear of the outlet 53 of the filtration tank 51 and opens and closes the outlet 53 in a timely manner to enable the supply to the raw material solution reservoir tank 71, and 74 denotes an electromagnetic valve for discharging water. is there. Reference numeral 75 denotes an electromagnetic valve attached to the pressing body 63 for introducing outside air to make the inside of the filtration tank 51 a positive pressure after the filtration. The end of the filtration is detected by a photoelectric sensor attached to a pipe between the raw material tank 31 and the filtering means 50. Reference numeral 76 denotes a remaining amount monitoring sensor attached to the raw material solution reservoir tank 71. The control unit 120 determines that the raw material solution in the raw material solution reservoir tank 71 has become equal to or less than a predetermined amount by a signal from this sensor. The raw material solution generating means 30 and the filtering means 50 are operated.

Under the control of the control unit 120, the raw material tank 31 and the filtration tank 51 are washed. That is, for example, after the filtered raw material solution is fed to the raw material solution reservoir tank 71, the electromagnetic valve 73 is closed and the electromagnetic valve 74 is opened, and the pure water supply pump 34 is driven to feed pure water. Draining and stopping are performed by opening and closing the solenoid valve 74, and the operation is repeated several times. The last washing water is stored in the filtration tank 51 until immediately before the tray moving mechanism 60 replaces the tray 55.

The enzyme reaction means 80 is configured as shown in FIGS. In the figure, reference numeral 81 denotes a reaction column filled with a water-insoluble immobilized carrier to which a raw material solution has passed and to which a decomposing enzyme has been bound, and which is fixed to the base 1. As the degrading enzyme, for example, pectin lyase, pectate lyase, polygalacturonase, or an esterase that hydrolyzes an ester of pectin is used. These enzymes are preferably used depending on pectin or pectic acid used as a raw material and mono- and oligogalacturonic acids required. However, in order to produce oligogalacturonic acid, it is better not to have an exo-type enzyme. Further, as the water-insoluble immobilization carrier, cellulose, agarose, polyacrylamide and the like are used.

Reference numeral 82 denotes a reaction column 8 through which the raw material solution stored in the raw material solution reservoir tank 71 is
1 is a raw material solution supply pump to be supplied to 1. The material solution supply pump 82 is constituted by a plunger pump capable of controlling a flow rate. Reference numeral 84 denotes a filter column provided in front of the reaction column 81, through which the raw material solution supplied from the raw material solution supply pump 82 passes and which has a smaller particle size than the water-insoluble immobilized carrier in the reaction column 81. Is filled. Reference numeral 85 denotes a buffer storage tank for washing. The buffer is sucked by a raw material solution supply pump 82 through an opening / closing solenoid valve 86 as required, and is supplied to the reaction column 81.
Supplied to

Reference numeral 90 denotes a temperature holding unit that holds the reaction column 81 at a constant temperature and also holds the raw material solution reservoir tank 71 at a constant temperature. As shown in FIGS. 2 and 12, the temperature holding unit 90 includes a constant temperature bath 91 for keeping tap water at a constant temperature,
A piping 92 for circulating the water in the thermostatic bath 91 around the reaction column 81 and around the raw material solution reservoir tank 71, and a circulation pump 93 interposed in the piping 92 and circulating the water in the thermostatic bath 91 are provided. Reference numeral 94 denotes a temperature sensor for detecting the temperature of the thermostatic bath 91, and reference numeral 95 denotes a heater and a cooler for heating and cooling based on the temperature detected by the temperature sensor. Reference numeral 96 denotes a water level sensor of the constant temperature bath 91, which supplies tap water so that a predetermined amount of water is always supplied to the constant temperature bath 91 based on the detection of the water level sensor 96 by the control unit 120.

The desalting means 100 is constructed as shown in FIGS. In the figure, 101, 10
Reference numeral 2 denotes a pair of ion exchange resin towers for desalination treatment, which are fixed to the base 1. 103 is an ion exchange resin tower 10
The reaction solution from the enzyme reaction means 80 can be supplied to either one of the ion exchange resin towers 101 and 102, and washing water for washing and regenerating the ion exchange resin can be supplied to one of the other ion exchange resin towers 101 and 102. This is a switching valve unit that alternately switches to The switching valve section 103 is constituted by a pair of 4-port rotary valves 105 driven to rotate by a motor 104. Reference numeral 106 denotes an electromagnetic valve for supplying and stopping the supply of the reaction solution from the enzyme reaction means 80, and reference numerals 107 and 108 denote on-off electromagnetic valves for taking out the reaction solution from the enzyme reaction means 80 to another part. 109 is a detector for detecting the production of mono- and oligogalacturonic acid.

Reference numeral 110 denotes a cleaning water supply section for supplying cleaning water for the ion exchange resin. The washing water supply unit 110 draws a corresponding solution from the caustic soda tank 111 for storing caustic soda, the hydrochloric acid tank 112 for storing hydrochloric acid, the pure water tank 6, the caustic soda tank 111 and the hydrochloric acid tank 112, and ion-exchange resin. A washing pump 113 for feeding the towers 101 and 102, and a motor-driven 6-port rotary for switching the pipeline so that the solution in the pure water tank 6, the caustic soda tank 111 and the hydrochloric acid tank 112 is selectively sucked by the washing pump 113. A valve 114 is provided. That is, the enzymatic reaction does not proceed unless some monovalent base is contained in the raw material solution serving as the substrate. Therefore, when preparing a raw material solution, an acetate buffer is added. This acetate buffer is present even if pectin is converted into mono- and oligogalacturonic acid by an enzymatic reaction. Therefore, an ion exchange resin is used to remove the pectin. This ion exchange resin loses its processing ability when a certain amount of base is constrained, but can be regenerated by washing with a high-concentration acid and alkali solution. Therefore, two columns packed with this ion exchange resin were prepared, and one was used to wash the other while the base was being removed with one.

Reference numeral 115 denotes an ion exchange resin tower 10 for desalination treatment.
A reaction solution reservoir tank for storing a desalinated reaction solution through 1, 102, a solenoid valve 116 for supplying and stopping the supply of the reaction solution to the reaction solution reservoir tank 115, and 117 for draining and draining the reaction solution An electromagnetic valve 118 for stopping is a water level sensor of the reaction solution reservoir tank 115, and is notified by the control unit 120 when a predetermined amount is reached. The control unit 120 controls the above-described units, drives each motor at a required timing, performs an opening / closing operation of each electromagnetic valve, or performs a required control based on detection of each sensor. It functions so that processing is performed in order.

Next, the operation of the apparatus for producing mono- and oligogalacturonic acids according to this embodiment will be described with reference to the flowcharts shown in FIGS. In advance, pectin or pectic acid powder is supplied as raw material supply means 1.
0 is stored in the hopper 11. The type of pectin used as a raw material is not limited as long as it is derived from plants, and the type of pectic acid is not limited as long as it is natural or obtained by pretreating pectin. When the device is started (1-
1) First, a pure water supply pump 34 is connected to the raw material tank 31 of the raw material solution generating means 30 from the pure water tank 6 of the pure water generating means 4.
, A predetermined amount of pure water is supplied (1-2). Next, the valve element 17 of the guide tube 15 of the raw material supply means 10 is opened, whereby the cap 33 of the raw material tank 31 is also opened, and the stepping motor 22 is driven to rotate the transfer rod 20 to convey the raw material. A predetermined amount of the raw material is supplied to the raw material tank 31 (1-3).

Here, the operation of the raw material supply means 10 will be described in detail. In the hopper 11, the stirring unit 23 is driven,
The raw material above the outlet opening 13 is agitated by the stirring blades 25 while the raw material is broken by the stirring rod 26, and the communication opening 15 a of the guide pipe 15 is formed from the outlet opening 13 of the hopper 11.
Sent to. In this case, even if the raw materials are strongly bonded to each other and stay in the hopper 11 and do not easily fall into the guide tube 15 from the outlet opening 13, they are finely loosened by the stirring rod 26 and the stirring blade 25, It surely falls from the outlet opening 13 to the guide tube 15. In addition, since the stirring rod 26 and the tips 25a and 25b of the stirring blades 25 rotate along the inner wall of the hopper 11, it is possible to prevent the raw material from adhering to the inner wall of the hopper 11 and stagnating. For this reason, the raw material is easily dropped from the hopper 11 to the guide tube 15, and the raw material is sufficiently supplied to the guide tube 15, so that the amount is not unstable and is supplied in a constant amount. Also, since the transfer rod 20 feeds the raw material by a screw, the amount of the raw material to be sent out is determined in proportion to the number of revolutions, so that a constant amount of the raw material is discharged. Further, since the valve 17 is provided, excess material is prevented from dropping into the material tank 31 when the motor 22 is stopped, and the amount of the material can be made accurate to the target amount.

When the raw material is put into the raw material tank 31, the stirring is performed by the stirring unit 44 for a predetermined time (1-1-
4). Further, the heater 45 is operated and is maintained at the predetermined temperature (1-7, 1-8). Therefore, the dissolution of the raw material is reliably performed. Next, a sodium acetate solution supply pump 38 is supplied from the sodium acetate solution tank 37 of the buffer solution supply section 36.
A predetermined amount of sodium acetate solution is supplied to the raw material tank 31
(1-5). Then, stirring by the stirring unit 44 is performed (1-6). During this time, the pH sensor 41
(1-9), and a necessary amount of the acetic acid solution is supplied from the acetic acid solution tank 39 to the raw material tank 31 by the acetic acid solution supply pump 40 so as to have a predetermined pH (1-10). . In addition, the entire amount of the raw material solution is detected (1-11), and pure water is supplied to a predetermined amount based on the detection (1-12). in this way,
Since the raw material solution is generated in predetermined amounts, it is possible to prevent a situation such as decay of the pectin solution, which is the raw material solution, from occurring.

Further, with the above-mentioned start, heating and cooling are performed by the heater and cooler device 95 in the constant temperature bath 91 of the temperature holding section 90 based on the temperature detection by the temperature sensor 94 so that the water reaches a predetermined temperature. (1-13,
1-14). Further, the water in the thermostatic bath 91 is circulated by the circulation pump 93 (1-15), and the temperatures of the reaction column 81 and the raw material solution reservoir tank 71 are kept constant.

When the raw material solution is generated, the solenoid valve 48 is opened (2-1, 2-2), and the filtration tank 51 of the filtration means 50 is opened.
The raw material solution flows down and filtration is performed (2-3). In this case, before the electromagnetic valve 48 is opened, the electromagnetic valve 74 is opened and the pure water for cleaning stored in the filtration tank 51 is drained (1-16). In addition, in the filtering means 50,
The tray 55 on which the filter paper 56 is placed is positioned at the inlet 52 of the filtration tank 51 by the tray moving mechanism 60, and is pressed against the opening of the inlet 52 by the pressing body 63 by the operation of the air cylinder 65. Then, by driving the aspirator 70, the raw material liquid is filtered by the filter paper 56.

When the filtration is completed, the solenoid valve 75 is opened to make the pressure inside the filtration tank 51 positive, and the solenoid valve is opened to open the filtration tank 5.
The processing solution in 1 is supplied to the raw material solution reservoir tank 71 by the pump 72 (2-4, 2-5). When the supply to the raw material solution reservoir tank 71 is completed, the raw material tank 31
Then, the filtration tank 51 is washed. This is because the electromagnetic valve 73 is closed and the electromagnetic valve 74 is opened, and the pure water supply pump 34
Is driven to feed pure water (2-6, 2-7). Draining and stopping are performed several times by opening and closing the solenoid valve 74, and the last washing water is stored in the filtration tank 51 (2-8, 2-9,
2-10).

The generation and filtration of the raw material solution are repeated. In this case, the replacement of the tray 55 is set before or during the generation of the solution by the raw material solution generating means 30. In this replacement, the air cylinder 65 is retracted, the pressing body 63 is retracted, the pressing of the tray 55 at the opening of the inlet 52 of the filtration tank 51 is released, and then the drive motor 62 of the tray moving mechanism 60 is driven. , Engaging member 61
Is performed by one rotation. As a result, the engaging member 61 moves the tray 55 located at the entrance 52, moves out of the entrance 52, drops the outlet 67, and then engages with the new tray 55 of the stacker 57 to move the tray 55. The new tray 55 is moved and positioned again at the entrance 52. Thereafter, the pressing by the pressing body 63 is performed by the advance of the air cylinder 65. In this way, since the filter paper 56 is replaced every time the raw material tank 31 is used, it is not necessary to use the dirty filter paper 56.
The accuracy of the filtration is improved, and the tray 55 on which the filter paper 56 is placed is simply placed in the stacker 57 so that the exchange is automatically performed, so that the work efficiency is greatly improved.

On the other hand, the raw material solution stored in the raw material solution reservoir tank 71 is supplied to the reaction column 81 through the filter column 84 by the raw material supply pump 82 with the electromagnetic valve 83 opened (2-11, 12). -12). In this case, since the filtration is further performed by the filter column 84,
The impurities are reliably removed and the quality of mono- and oligogalacturonic acids is improved accordingly. Then, in the reaction column 81, the raw material pectin or pectic acid is decomposed into mono- and oligogalacturonic acid by the decomposing enzyme immobilized on the water-insoluble immobilization carrier. In this case, since the flow rate of the raw material solution supply pump 82 can be changed, the enzyme reaction rate can be adjusted by adjusting the flow rate, and desired conditions can be set. Further, since the reaction column 81 and the raw material solution reservoir tank 71 are kept at a predetermined temperature, the enzyme reaction rate can be adjusted by setting the temperature, and desired conditions can be set.
Therefore, decomposition into mono- and oligogalacturonic acids can be reliably performed. That is, since the flow rate of the pectin or pectic acid solution sent to the reaction column 81 and the temperature of the reaction column 81 are controlled, the degree of polymerization of the mono- and oligogalacturonic acids obtained by this enzymatic reaction is determined to be the desired degree of polymerization. It can be set to have a distribution.

Next, the reaction solution from the reaction column 81 is:
It is sent to the desalination processing means 100 via the electromagnetic valve 106 (2-13). In this case, the production of mono- and oligogalacturonic acid is detected by the detector 109 (1-14),
When the generation is not performed, the electromagnetic valve 107 is opened to drain the water (2-15). When the generation is performed, the electromagnetic valve 107 is closed (2-16), and the electromagnetic valve 106 is opened to open the desalination processing means 100. (2-16). It is also possible to open the solenoid valve 108 by manual switching and take it out for other processing (2-17, 2-18).

In the desalting means 100,
The reaction solution is passed through a 4-port rotary valve 105 (3-1) into one of the ion-exchange resin towers 101 (in the case shown) (3-2), and subjected to a desalination treatment.
After passing through the 4-port rotary valve 105 (3-4), it is stored in the reaction solution reservoir tank 115 via the electromagnetic valve 116 (3-5, 3-7). In the other ion-exchange resin tower 102 (in the case shown) (3-3), washing water is passed through to perform washing, and then drained (3-7, 3-8).
The reaction solution can be drained by opening the solenoid valve 117. Thereby, regeneration of the ion exchange resin is performed. Wash water is supplied as follows. At a predetermined timing (3-9), first, a predetermined amount of hydrochloric acid is supplied by the cleaning pump 113, and pure water supplied in advance is drained from any of the ion exchange resin towers 102 (3-10, 3-11). ), Hydrochloric acid is retained for a predetermined time (3-12). Next, the 6-port rotary valve 11
4 is switched (3-13), pure water is supplied by the washing pump 113 to wash the ion-exchange resin tower 102 (3-14, 3-15), and is kept for a predetermined time (3-16). . Next, the 6-port rotary valve 114 is switched (3-17), and a predetermined amount of caustic soda is supplied by the cleaning pump 113 and retained for a predetermined time (3-18, 3-19).

Thereafter, the 6-port rotary valve 114
Is switched (3-20), and pure water is supplied by the washing pump 113 to wash the ion exchange resin tower 102 (3-21, 3-22). Thereafter, the 6-port rotary valve 114 is switched again (3-23), and a predetermined amount of hydrochloric acid is supplied by the cleaning pump 113 and is retained for a predetermined time (3-24, 3-25). Finally, the 6-port rotary valve 114 is switched (3-26), and pure water is supplied by the washing pump 113 to wash the ion exchange resin tower 102 (3-2).
Along with 7, 3-28). Thus, the ion exchange resin is regenerated. The desalting treatment and the washing treatment are performed in the ion exchange resin tower 1
01 and 102 are performed alternately.

[0044]

Next, a more detailed embodiment will be described.
In the pure water generation, in the direct water supply system, impurities contained in the tap water are removed by a filter and an ion exchange resin,
Approximately 2 μS of pure water could be processed with a capacity of 100 (l / h). Ion exchange resin is cartridge type,
The electric conductivity of pure water was constantly measured by a simple electric conductivity meter, and it was replaced when the processing capacity decreased. The throughput of the cartridge is about 3000 liters. In order to minimize the reduction in the processing capacity of the ion exchange resin, a filter of 5 μm was provided in front of the ion exchange resin cartridge. Further, a 0.5 μm filter was attached to the subsequent stage of the ion-exchange resin cartridge in order to avoid contamination of impurities when the processing capacity of the cartridge was reduced. Here, the created pure water is used to make a pectin solution as a raw material solution,
Used for regeneration of ion exchange resin for desalination. In particular, since it is used in large quantities for the regeneration of ion exchange resins,
0 liters can be stored, and the solenoid valve between the water tap and the preceding filter is turned on and off by a contact type remaining amount sensor provided in the storage material tank 31 so that the tank is always full.

In the raw material supply means 10, the following experiment was conducted to confirm the stable supply capability of the powder. 500 g of pectin powder is put into the hopper 11, and the transfer rod 20 (the inner diameter of the guide tube 15 is 22 mm, and the rod diameter is 20 m).
m, a thread portion (lead 7 mm, three-start thread)) is 540 by a stepping motor 22 which rotates 0.72 degrees per pulse.
The amount of repetitive supply when set to supply 5 g by rotating 0 pulses was measured with an electronic balance (Shimadzu EB-300H). Further, the stepping motor 22 is set to 1080, 2
160, 3240, 4320, and 5400 pulses were rotated, and the supply amount at that time was measured. The following results were obtained.

The supply amount when the stepping motor 22 was rotated 5400 pulses was measured 30 times, and the average value was 5.0.
A good result of 67 g and a standard deviation of 0.0328 was obtained. Also, the stepping motor 22 is set to 1080, 216
The result of regression analysis of the relationship between the number of rotations of the stepping motor 22 and the amount of supply when the amount of supply at the time of 0, 3240, 4320, and 5400 pulse rotations was measured ten times, and the average value was used as the amount of supply at that number of rotations. , Correlation function 1.
00, indicating that the desired supply amount can be stably obtained by making the rotation speed variable. Therefore, it was possible to generate a raw material solution with high accuracy.
FIG. 17 shows the result of regression analysis of the relationship between the rotation speed of the stepping motor 22 and the supply amount.

In the production of the raw material solution, the amount produced at one time was 1 liter, and it could be produced automatically from pectin powder by the following procedure. 800 ml of pure water is injected into the raw material tank 31 by the quantitative pure water supply pump 34, 5 g of pectin powder is supplied thereto, and the mixture is heated to 60 ° C. by the heater 45 and stirred for 30 minutes. 30 ml of 1 M acetic acid solution
While injecting, acetic acid is injected so that the pH of the solution becomes 4.3 while measuring with the pH sensor 41. Finally, pure water was injected so that the volume became 1 liter, so that a 0.5% pectin solution was obtained.

In the filtering means 50, the filtration of the pectin solution is performed in accordance with No. Using a paper filter (filter paper 56) of 5A (Toyo Roshi Kaisha, Ltd.), suction filtration is performed with an aspirator 70. Generally, when a pectin solution is filtered, the paper filter becomes clogged.
The tray was changed automatically each time a liter of pectin solution was processed. The number of stocked trays for replacement was set to 10, and when the remaining amount of the tray became 3 or less, the remaining amount sensor was activated and an alarm was issued.

[0049] In the enzyme reaction means 80, the enzyme used in the reaction column 81 is made of Sterum pulp.
(pureum) is cultured in a potato broth medium containing 1% glucose for 3 weeks, and is a pectin-degrading enzyme obtained by a conventional method from the culture filter paper, and 250 μg of this enzyme is dissolved in 0.1 M HEPES buffer (pH 7.5). To 5 ml of a water-insoluble immobilized carrier Affigel 10 (trade name, manufactured by Nippon Bio-Rad Laboratories, Inc.) and packed in a reaction column 81. In addition, the structure was such that water maintained at a constant temperature in the thermostatic bath 91 was circulated, and the enzyme reaction rate was controlled by the temperature of the circulating water. The enzyme reaction rate was also controlled by changing the flow rate of the pectin solution flowing in the reaction column 81. The flow rate of the pectin solution is 0 to 9.99 (ml / mi) by a plunger type pump.
n) can be varied with an accuracy of 0.01 (ml / min), and can be controlled by the control unit 120 using RS232C. Further, a pressure sensor is provided in the plunger pump to detect an abnormality such as clogging of the reaction column 81 or the filter column 84, and the indicated value of this sensor is monitored by the control unit 120. Stopped.

The thermostat 91 of the temperature holding section 90 has a capacity of 2
In order to minimize the influence of the outside air temperature, a double structure was used, and a glass fiber heat insulating material was used in the middle.
Thereby, the temperature set in the range of 5 to 60 ° C. can be kept constant. The circulation pump 93 is 10
(L / min) pump was used. Desalination means 10
The ion exchange resin used at 0 was capable of processing 20 liters of the reaction solution at one time. The pectin solution fed into the reaction column 81 is decomposed into mono- and oligogalacturonic acids by an enzymatic reaction, and is sent to a column filled with a cation exchange resin Apanbarlite (trade name, Nippon Organo Co., Ltd.) to be desorbed. Salted. Using polygalacturonic acid as a raw material, a reaction column 81
The average polymerization degree of mono- and oligogalacturonic acids obtained when the flow rate of the mixture fed to the reactor was 1.0 (ml / min) and the temperature of the reaction column 81 was set at 25 ° C. was about 7. The average degree of polymerization is determined by measuring the amount of reducing terminals of mono- and oligogalacturonic acids by a color reaction according to the Milner-Avigado method, measuring the total amount of galacturonic acid by a colorimetric reaction by the galvazole-sulfuric acid method, and determining the total amount of galacturonic acid. It was determined by dividing by the amount of reducing terminal. FIG. 18 shows the reaction column 8
1 shows the relationship between the flow rate and the average degree of polymerization.

The control section 120 controls the operation of each means by a sequencer. The status of each means is
The data is sequentially transmitted to the general monitoring means using serial communication by RS232C. In addition, switches and lamps for confirming the operation of each means are eliminated by using a liquid crystal display, and the surface of the housing is made cleaner. In addition, visibility and operability are improved by arranging touch switches on the liquid crystal panel. In addition, if a power failure occurs during operation, it is necessary to manually perform pectin solution preparation and return to the origin of the pulse motor used for pectin solution preparation. An uninterruptible power supply was provided.

[0052]

As described above, according to the apparatus for producing mono- and oligogalacturonic acid of the present invention, a solution is automatically formed from pectin or pectic acid powder, and the solution is automatically filtered to decompose pectin or pectic acid. It is possible to automatically and continuously produce mono- and oligogalacturonic acids having a desired degree of polymerization by feeding a reaction column filled with a water-insoluble resin having an enzyme immobilized thereon. Therefore, the pre-treatment step, the reaction step, and the post-treatment step can be automatically performed, and the production efficiency can be improved, and the mass production can be stably performed with little labor. Further, since a predetermined amount of the raw material solution is generated from a predetermined amount of powder, it is possible to prevent a situation such as decay of the pectin solution, which is the raw material solution, from occurring, and to surely produce mono- and oligogalacturonic acids. it can.

Further, the material supply means is provided with an externally threaded transfer rod which is rotated about the axis of the guide tube and is rotated in the guide tube, and the material supplied from the hopper by this rotation is supplied to the discharge port of the guide tube. Since the transfer rod is configured to transfer the raw material , the male screw-shaped transfer rod has a constant space into which the raw material enters, and therefore, by controlling the rotation speed of the motor, the supply amount of the raw material can be made accurate. ,
An accurate raw material solution can be generated. Further, since it is not necessary to measure the weight, the apparatus can be simplified and the cost can be reduced.

Further, the raw material solution generating means includes a pure water supply unit for supplying pure water into the raw material tank, a buffer liquid supply unit for supplying a buffer solution into the raw material tank, a raw material supplied to the raw material tank, Since the system is provided with a stirrer for stirring pure water and a buffer , pure water and a buffer are separately added.
Adjustment of H and the like become easy, and a predetermined amount of raw material solution can be reliably generated from a predetermined amount of powder. In addition, since a heater for heating the solution in the raw material tank is provided , the solubility of the raw material can be increased, and in this respect, the raw material solution can be reliably generated.

[0055] Furthermore, this the filtering means, configured as a tray carrying the filter paper can be replaced by a tray moving mechanism
From may not use a dirty filter paper, therefore, along with the precision of filtration is improved, only keep installed trays stacker, since automatic exchange is performed, significantly improving the working efficiency And the performance of automation can be improved.

Further, since the enzyme reaction means is provided with a reaction column packed with a water-insoluble immobilized carrier to which a raw material solution has passed and to which a decomposing enzyme has been bound, the operation is simpler than that of a badge type. Be able to mass produce. In this case, whether a filter column was provided in front of the reaction column
Further, since filtration is further performed in addition to the filtration by the above-mentioned filtration means, impurities can be surely removed, and thereby contamination of the degrading enzyme can be prevented, so that the quality of mono- and oligogalacturonic acids can be improved. it can. Furthermore,
Since it has a temperature holding unit that holds the reaction column at a constant temperature ,
You can adjust the enzyme reaction rate by setting the temperature,
You can set the desired conditions. Therefore, decomposition into mono- and oligogalacturonic acids can be reliably performed. That is, the degree of polymerization of the mono- and oligogalacturonic acids obtained by the enzymatic reaction can be set so as to have a desired distribution of the degree of polymerization.

Further, the desalting means is provided so that the reaction solution from the enzyme reaction means is supplied to one of the pair of ion exchange resin towers and the washing water is supplied to one of the other ion exchange resin towers. Because of the configuration , the ion-exchange resin towers can be used alternately, so that operations such as replacing the ion-exchange resin towers for the regeneration one by one become unnecessary, and therefore, the performance of automation can be further improved. .

[Brief description of the drawings]

FIG. 1 is a perspective view showing an apparatus for producing mono- and oligogalacturonic acid according to an embodiment of the present invention.

FIG. 2 is a view showing an apparatus for producing mono- and oligogalacturonic acids according to an embodiment of the present invention.

FIG. 3 is a view showing a pure water generating means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 4 is a view showing a raw material supply means and a raw material solution generating means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 5 is a sectional view showing a raw material supply means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 6 is a cross-sectional perspective view of a main part showing a raw material supply unit of the apparatus for producing mono- and oligogalacturonic acids according to the embodiment of the present invention.

FIG. 7 is a view showing a filtering means of the apparatus for producing mono- and oligogalacturonic acids according to the embodiment of the present invention.

FIG. 8 is a perspective view showing a filtering means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 9 is a cross-sectional view showing a filtering means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 10 is a perspective view of a main part showing a filtering means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 11 is a diagram showing an enzyme reaction means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 12 is a view showing a temperature holding unit of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 13 is a diagram showing a desalting means of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 14 is a flowchart illustrating the operation of the apparatus for producing mono- and oligogalacturonic acids according to the embodiment of the present invention.

FIG. 15 is a flowchart showing the operation of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 16 is a flowchart showing the operation of the apparatus for producing mono- and oligogalacturonic acid according to the embodiment of the present invention.

FIG. 17 is a graph showing the relationship between the number of rotations of the motor and the supply amount in the raw material supply means of the apparatus for producing mono- and oligogalacturonic acids according to the example of the present invention.

FIG. 18 is a graph showing the relationship between the flow rate of the solution in the reaction column and the average degree of polymerization in the enzyme reaction means of the apparatus for producing mono- and oligogalacturonic acids according to the example of the present invention.

[Explanation of symbols]

 Reference Signs List 1 base 2 panel 3 frame 4 pure water generating means 5 pure water device 6 pure water tank 7, 8 filter 10 raw material supply means 11 hopper 12 supply opening 13 outlet opening 14 lid 15 guide pipe 15a communication port 16 discharge port 17 valve body Reference Signs List 18 Air cylinder 18a Mounting member 19 Solenoid valve 20 Transfer rod 20a Base end 21 Bearing 22 Stepping motor 22a Motor shaft 23 Stirrer 24 Vertical rotation shaft 25 Stirrer blade 25a Tip 25b Tip 26 Stirrer rod 26a Falling blade 27 Drive mechanism And 28 gear mechanism 28a bearing portion 28b horizontal rotating shaft 28c driving bevel gear 28d driven bevel gear 29 belt transmission mechanism 29a driving pulley 29b driven pulley 29c belt 30 is a raw material solution generating means 31 raw material tank 32 inlet 33 cap 33a connecting rod 33b O −Ring 34 Pure water supply pump 35 Sensor 36 Buffer supply unit 37 Sodium acetate solution tank 38 Sodium acetate solution supply pump 39 Acetate solution tank 40 Acetate solution supply pump 41 pH sensor 42, 43 Liquid level sensor 44 Stirring unit 44a Motor 44a 44b Stirring fan 45 Heater 46 Temperature sensor 47 Photoelectric sensor 48 Solenoid valve 50 Filtration means 51 Filtration tank 52 Inlet 53 Outlet 54 Base member 55 Tray 55a Through hole 56 Filter paper 57 Stacker 58 Top plate 59 Extraction opening 60 Tray moving mechanism 61 Engaging member 62 Drive motor 63 Pressing body 64 Inlet passage 65 Air cylinder 66 Stand 67 Discharge port 68 Sensor 70 Aspirator 71 Raw material reservoir tank 72 Pump 73 Solenoid valve 74 Solenoid valve 75 Solenoid valve 76 Remaining amount monitoring sensor 8 Enzyme reaction means 81 Reaction column 82 Raw material solution supply pump 83 Opening / closing solenoid valve 84 Filter column 85 Buffer storage tank 86 Opening / closing solenoid valve 90 Temperature holding section 91 Constant temperature bath 92 Piping 93 Circulation pump 94 Temperature sensor 95 Heater, cooler device 96 Water level Sensor 100 Desalination means 101, 102 Ion exchange resin tower 103 Switching valve unit 104 Motor 105 4-port rotary valve 106 Solenoid valve 107, 108 Open / close solenoid valve 109 Detector 110 Wash water supply unit 111 Caustic soda tank 112 Hydrochloric acid tank 113 Wash Pump 114 6-port rotary valve 115 reaction solution reservoir tank 116 solenoid valve 117 solenoid valve 118 water level sensor 120 control unit

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Takeshi Takeshi, Aomori City, Aomori Prefecture, Yatsuo 202-4, Ashiya 202-4 Aomori Prefecture Industrial Technology Development Center (72) Inventor, Norimitsu Hanamatsu Aomori City, Aomori Prefecture 4 in the Atsuya 202, Aomori Prefecture Industrial Technology Development Center (72) Inventor Fujitoshi Shinoki, in the Aomori City, Aomori City, Aomori 2024 in the Aomori Prefecture Industrial Technology Development Center (72) Inventor Shunichi Mikami 5-1, Oaza Metal Town, Hirosaki City, Aomori Prefecture Towa Denki Kogyo Co., Ltd. (72) Inventor Hideyuki Nara 5-1, Oji Metal Town, Hirosaki City, Hirosaki City, Aomori Prefecture (JP, A) (58) Field surveyed (Int. Cl. ⁶ , DB name) C12P 19/14 C12M 1/40

Claims (1)

(57) [Claims] 1. An apparatus for producing mono- and oligogalacturonic acid using pectin or pectic acid powder as a raw material, a raw material supply means for supplying a predetermined amount of the raw material, and a buffer solution added to the raw material supplied from the raw material supply means. A raw material solution generating means for generating a predetermined amount of the raw material solution, and a filtering means for filtering the raw material solution generated by the raw material solution generating means,
Enzymatic reaction means for performing an enzymatic reaction with a water-insoluble immobilized carrier having a decomposing enzyme bound to the raw material solution filtered by the filtration means; and desalting of the reaction solution having the enzymatic reaction performed by the enzymatic reaction means. Bei a desalination treatment means for processing
A source hopper for storing the source material;
A guide that guides the raw material supplied from the outlet opening of the hopper
A pipe and an axis of the guide pipe as a rotation axis and in the guide pipe.
The raw material supplied by this rotation is
Male threaded transfer rod for transfer to the discharge port of the id tube
And a motor for rotating the transfer rod by a predetermined number of rotations.
Ete configure, the raw material solution generating means, supplied of from the raw material supply means
Raw material tank for storing the raw material
A pure water supply unit for supplying water and a buffer solution in the raw material tank
And a buffer solution supply section for supplying
A stirrer for stirring the raw material, pure water and buffer solution
And a heater for heating the solution in the raw material tank, wherein the filtering means is provided with a raw material generated by the raw material solution generating means.
Inlet for the feed solution and an outlet that is opened and closed in a timely manner
A filtration tank with an opening, and a
If a number of holes are opened and a supporting surface for supporting the filter paper is provided
To be moved to the inlet of the filtration tank
And a stacker that stocks multiple trays
The tray placed at the inlet of the filtration tank is removed from the inlet
Exit and remove a new tray from the stacker.
A tray moving mechanism for taking out and moving to the entrance,
An aspirator that sucks the inside of the filtration tank
And, the enzymatic reaction unit, the enzyme the raw material solution is passed through
Reaction column filled with bound water-insoluble immobilized carrier
And a raw material solution feed for feeding the raw material solution to the reaction column.
And a pump, and at the front of the reaction column,
Water insoluble in the above reaction column when the fed raw material solution passes
Filling a carrier with a smaller particle size than the immobilized carrier
Providing a filter column for filtering the liquid, comprising a temperature holding section for holding the reaction column at a constant temperature , the desalination means, a pair of ion exchange resin for desalination treatment
Column and one of the ion-exchange resin towers.
The reaction solution from the
The ion exchange resin is washed and regenerated in the on-exchange resin tower.
Switching valve that alternately switches to enable the supply of cleaning water
And a washing water supply unit for supplying washing water for the ion exchange resin.
And an apparatus for producing mono- and oligogalacturonic acid.