JP2883213B2 - Biotransplant material and its manufacturing method - Google Patents
Biotransplant material and its manufacturing methodInfo
- Publication number
- JP2883213B2 JP2883213B2 JP2416119A JP41611990A JP2883213B2 JP 2883213 B2 JP2883213 B2 JP 2883213B2 JP 2416119 A JP2416119 A JP 2416119A JP 41611990 A JP41611990 A JP 41611990A JP 2883213 B2 JP2883213 B2 JP 2883213B2
- Authority
- JP
- Japan
- Prior art keywords
- chitosan
- collagen
- solution
- calcium phosphate
- implant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Materials For Medical Uses (AREA)
- Dental Preparations (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、生体用移植材に関する
ものであり、更に詳しくは歯周骨欠損等、骨、歯等の硬
組織に使用する生体用移植材とその製造方法に関するも
のである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an implant for living body, and more particularly to an implant for living body used for hard tissues such as bones and teeth such as periodontal bone defects, and a method for producing the implant. is there.
【０００２】[0002]
【従来の技術】骨折や骨腫瘍の手術のために骨欠損や空
隙が生じることがある。このような欠損部や空隙部を埋
めるために、近年人工の骨充填材料が開発され、使用さ
れている。 人工の骨充填材料の材質としては金属やセ
ラミックスが知られているが、これらのかち生体適合性
の観点から、好ましいものはリン酸カルシウム系化合物
である。その中でもハイドロキシアパタイト、トリカル
シウムフォスフェートが現在、広く使用されており、多
くの動物実験、一般臨床に使用されている。又、キトサ
ン、コラーゲン、ポリ乳酸等の高分子材料とリンカルシ
ウム系化合物体との複合化の研究が行われている。キト
サンには骨増成の有効性はなく、骨欠損部へのリン酸カ
ルシウム系化合物体補綴時での操作性改善の為に研究さ
れている。コラーゲンは、坑原性を有する為にリン酸カ
ルシウム系化合物体との複合化方法、その含有量につい
て研究されている。2. Description of the Related Art Bone defects and voids may occur due to surgery for fractures and bone tumors. In recent years, artificial bone filling materials have been developed and used to fill such defective portions and voids. Metals and ceramics are known as materials for the artificial bone filling material, and among these, a calcium phosphate compound is preferred from the viewpoint of biocompatibility. Among them, hydroxyapatite and tricalcium phosphate are currently widely used, and are used in many animal experiments and general clinical practice. In addition, research has been conducted on complexing a polymer material such as chitosan, collagen, polylactic acid, and the like with a calcium phosphate compound. Chitosan is not effective for bone augmentation, and has been studied to improve the operability during prosthesis of a calcium phosphate compound to a bone defect. Collagen has been studied for its method of complexing with a calcium phosphate compound and its content because it has antigenic properties.
【０００３】[0003]
【従来技術の課題】従来の顆粒状やブロック状のリン酸
カルシウム系化合物の移植材では、充填した後に、移植
物が移動し、欠損部からはみ出したりする。又移植材の
固定が不十分な為に、移植材周辺での新生骨や結合組織
の生成が不十分である。本発明は、骨欠損部への移植材
が動揺したり、はみだしたりすることなく、周辺での新
生骨や結合組織の生成も十分な移植材を提供することを
目的としている。2. Description of the Related Art In a conventional granulated or block-shaped calcium phosphate compound transplant, after filling, the transplant moves and protrudes from the defect. In addition, because of insufficient fixation of the graft material, formation of new bone and connective tissue around the graft material is insufficient. An object of the present invention is to provide a graft material that does not shake or protrude a graft material into a bone defect, and that can sufficiently generate new bone and connective tissue in the periphery.
【０００４】[0004]
【課題を解決するための手段】本発明者は、人工骨の充
填材料として、使用しているリン酸カルシウム系化合物
体に、キトサン・コラーゲン複合体皮膜を包囲させるこ
とによって、移植材の動揺を防ぐことを見出した。すな
わち、本発明は包囲したキトサン・コラーゲン複合体皮
膜が移植後、膨潤し、リン酸カルシウム系化合物体を固
定させることのできる生体移植材を提供するものであ
る。Means for Solving the Problems The present inventor has proposed a method of preventing a transplantation material from shaking by surrounding a calcium phosphate compound used as a filling material for artificial bone with a chitosan-collagen composite film. Was found. That is, the present invention provides a living body transplant material in which the enclosed chitosan / collagen composite film swells after transplantation and can fix the calcium phosphate compound.
【０００５】本発明におけるリン酸カルシウム系化合物
体の好ましい具体例としては、ハイドロキシアパタイ
ト、トリカルシウムフォスフェート、リン酸カルシウム
系生体ガラスがある。Preferred examples of the calcium phosphate compound in the present invention include hydroxyapatite, tricalcium phosphate, and calcium phosphate bioglass.
【０００６】本発明に使用するキトサン・コラーゲン複
合溶液はキトサンを２〜20wt％、コラーゲンを0.1 〜1.
0wt ％含んだものである。キトサンを溶解させる液とし
ては、リン酸、塩酸、酢酸、ギ酸、乳酸、リンゴ酸、ク
エン酸等がある。コラーゲン溶液が0.1wt ％以下では周
辺での新生骨や結合組織の生成が不十分である。又1wt
％以上ではコラーゲンの抗原性の徴候があらわれてく
る。以上からコラーゲンの量を0.1 〜1.0wt ％とした。
キトサン量が２wt％以下では、濃度が薄い為に均一なコ
ーティングが困難である。又２0wt ％以上では、粘性が
高く、多孔質体への含浸が困難である。以上からキトサ
ンの濃度を２〜２0wt％とした。以下、本発明を実施例
によりさらに具体的に説明する。The chitosan / collagen composite solution used in the present invention contains 2-20% by weight of chitosan and 0.1-1.
It contains 0 wt%. Examples of the solution for dissolving chitosan include phosphoric acid, hydrochloric acid, acetic acid, formic acid, lactic acid, malic acid, and citric acid. If the collagen solution is less than 0.1 wt%, the formation of new bone and connective tissue in the periphery is insufficient. 1wt
Above 10%, signs of collagen antigenicity appear. From the above, the amount of collagen was set to 0.1 to 1.0 wt%.
If the amount of chitosan is less than 2% by weight, uniform coating is difficult due to the low concentration. If the content is more than 20% by weight, the viscosity is high and it is difficult to impregnate the porous body. From the above, the concentration of chitosan was set to 2 to 20% by weight. Hereinafter, the present invention will be described more specifically with reference to examples.
【０００７】[0007]
【実施例】実施例１ キトサン10g 、コラーゲン0.1gをPH4.0 のリン酸溶液10
0 ｍｌに溶解し、キトサン−コラーゲン複合溶液を得
た。この液に200 〜1000μの顆粒状トリカルシウムフォ
スフェートを浸漬、乾燥後、過剰なリン酸を洗浄し、ト
リカルシウムフォスフェートをキトサン・コラーゲン複
合体皮膜で包囲した移植材を得た。 EXAMPLE 1 10 g of chitosan and 0.1 g of collagen were added to a phosphoric acid solution of PH 4.0.
This was dissolved in 0 ml to obtain a chitosan-collagen composite solution. After 200-1000 μg of granular tricalcium phosphate was immersed in this solution and dried, the excess phosphoric acid was washed to obtain a graft material in which tricalcium phosphate was surrounded by a chitosan / collagen composite film.
【０００８】作製した移植材をビーグル犬の下顎骨に充
填し、組織学的検索を行った。２ケ月後に犬を屠殺し、
観察したところ、移植材の周囲及び間隔に結合組織、新
成骨が認められた。[0008] The prepared implant was filled into the mandible of a beagle dog, and histological examination was performed. Two months later the dog was slaughtered,
Upon observation, connective tissue and new bone were found around and at the interval of the implant.
【０００９】実施例２ キトサン５g 、コラーゲン１g をPH5.0 のリン溶液100
ｍｌに溶解し、キトサン・コラーゲン複合溶液を得た。
この液に平均孔径200 μの多孔体のリン酸カルシウム系
生体ガラス体に浸漬し、水酸カルシウムにて中和、乾燥
し、生体ガラス体をキトサン・コラーゲン複合体皮膜で
包囲した移植材を得た。 Example 2 5 g of chitosan and 1 g of collagen were added to a phosphoric acid solution of PH5.0 in 100
The resulting mixture was dissolved in the resulting solution to obtain a chitosan / collagen composite solution.
This liquid was immersed in a porous calcium phosphate-based living glass body having an average pore diameter of 200 μm, neutralized with calcium hydroxide, and dried to obtain an implant material in which the living glass body was surrounded by a chitosan / collagen composite film.
【００１０】作製した移植材をビーグル犬の大腿骨に充
填し、組織学的検索を行った。１ケ月後に犬を屠殺し、
観察したところ、移植材のポア内には、旺盛な骨増成が
観察された。[0010] The prepared implant material was filled in a femur of a beagle dog, and histological examination was performed. A month later the dog was slaughtered,
Upon observation, vigorous bone augmentation was observed in the pores of the implant.
【００１１】実施例３ キトサン20g 、コラーゲン0.1gをPH4.0 の酢酸溶液100
ｍｌに溶解し、キトサン・コラーゲン溶液を得た。この
液に200 〜2000μのハイドロキシアパタイト顆粒を浸
漬、乾燥後、過剰な酢酸を洗浄、乾燥し、ハイドロキシ
アパタイトをキトサンコラーゲン複合体皮膜で包囲した
移植材を得た。実験的に歯周病をおこさせた、ビーグル
犬の上顎の第４小臼歯部周囲に、上記の移植材を充填
し、観察を行った。 Example 3 20 g of chitosan and 0.1 g of collagen were added to an acetic acid solution of PH 4.0 in 100
The resulting solution was dissolved in the solution to obtain a chitosan / collagen solution. Hydroxyapatite granules of 200 to 2000 µ were immersed in this solution and dried, and then excess acetic acid was washed and dried to obtain a graft material in which hydroxyapatite was surrounded by a chitosan collagen composite film. The above implant was filled around the fourth premolar region of the upper jaw of a beagle dog that had experimentally caused periodontal disease, and observation was performed.
【００１２】３ケ月までの観察では、ハイドロキシアパ
タイトの顆粒の流出はなく、又移植材の動揺もレントゲ
ン検査では観察されなかった。屠殺し、組織学的検索を
行った。その結果歯の周囲には新成骨の増成がみられ、
良好な歯周支持組織が生成していることが認められた。Up to three months, no hydroxyapatite granules flowed out, and no fluctuation of the implant was observed by X-ray examination. Sacrifice and histological searches were performed. As a result, new bones grow around the teeth,
It was confirmed that a good periodontal support tissue was generated.
【００１３】[0013]
【発明の効果】本発明のリン酸カルシウム系化合物体を
キトサン・コラーゲン複合体皮膜で包囲した生体移植材
は、生体にとって毒性はなく、移植材の動揺もなく、周
辺での新成骨や結合組織の増成がみられる。したがって
本発明による移植材は、歯科及び整形外科分野において
極めて有用である。The biological graft material comprising the calcium phosphate compound of the present invention surrounded by a chitosan / collagen composite film has no toxicity to the living body, does not shake the graft material, and has no effect on the formation of new bone and connective tissue in the vicinity. There is an increase. The implant according to the invention is therefore very useful in the fields of dentistry and orthopedics.
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.6，ＤＢ名) A61L 27/00 ──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int.Cl. 6 , DB name) A61L 27/00
Claims (2)
ン・コラーゲン複合体皮膜で包囲した生体移植材。1. A biotransplant comprising a calcium phosphate compound surrounded by a chitosan / collagen composite film.
域で溶解したキトサン・コラーゲン複合溶液中に浸漬
し、乾燥後脱酸処理する工程を含む生体移植材の製造方
法。2. A method for producing a biograft, comprising the steps of immersing a calcium phosphate compound in a chitosan / collagen complex solution in an acidic region, drying and deoxidizing the solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2416119A JP2883213B2 (en) | 1990-12-28 | 1990-12-28 | Biotransplant material and its manufacturing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2416119A JP2883213B2 (en) | 1990-12-28 | 1990-12-28 | Biotransplant material and its manufacturing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04242658A JPH04242658A (en) | 1992-08-31 |
| JP2883213B2 true JP2883213B2 (en) | 1999-04-19 |
Family
ID=18524361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2416119A Expired - Fee Related JP2883213B2 (en) | 1990-12-28 | 1990-12-28 | Biotransplant material and its manufacturing method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2883213B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4302708C2 (en) * | 1993-02-01 | 1995-06-01 | Kirsch Axel | Covering membrane |
| JP3931134B2 (en) * | 2002-10-31 | 2007-06-13 | 泰彦 田畑 | Implant for living tissue regeneration and method for producing the same |
| JP2005245800A (en) * | 2004-03-05 | 2005-09-15 | Hosoda Denki:Kk | Acidic water containing promotion agent, acidic water containing implant, and mouth wash liquid |
| CN114681667A (en) * | 2022-02-16 | 2022-07-01 | 中南大学湘雅三医院 | Preparation method of drug-loaded sustained-release stent for filling bone defects with anti-osteosarcoma |
-
1990
- 1990-12-28 JP JP2416119A patent/JP2883213B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04242658A (en) | 1992-08-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |