JP2845287B2 - Immersion oil for microscope - Google Patents
Immersion oil for microscopeInfo
- Publication number
- JP2845287B2 JP2845287B2 JP2262276A JP26227690A JP2845287B2 JP 2845287 B2 JP2845287 B2 JP 2845287B2 JP 2262276 A JP2262276 A JP 2262276A JP 26227690 A JP26227690 A JP 26227690A JP 2845287 B2 JP2845287 B2 JP 2845287B2
- Authority
- JP
- Japan
- Prior art keywords
- immersion oil
- microscope
- refractive index
- objective lens
- plastic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、高屈折率であり、かつ透明性、粘度、安全
性等の諸特性が良好な顕微鏡用液浸油に関する。Description: TECHNICAL FIELD The present invention relates to a microscope immersion oil having a high refractive index and excellent properties such as transparency, viscosity and safety.
〔従来の技術〕 従来、顕微鏡用液浸油としては、1−フェニル−1−
(3,4−ジメチルフェニル)エタン、1,2−ビス(α−メ
チルベンジル)−4,5−ジメチルベンゼン、2,4−ジフェ
ニル−4−(3,4−ジメチルフェニル)ブタン等の化合
物と流動パラフィンとの混合物や、特開昭63−151913号
公報及び特開昭63−174009号公報に示されているよう
に、フタル酸エステル類とパラフィンまたはポリブテン
との混合物が用いられている。また、高屈折率用の顕微
鏡用液浸油としては、1−ブロムナフタリン等が一般的
である。[Prior art] Conventionally, as immersion oil for microscopes, 1-phenyl-1-
Compounds such as (3,4-dimethylphenyl) ethane, 1,2-bis (α-methylbenzyl) -4,5-dimethylbenzene, and 2,4-diphenyl-4- (3,4-dimethylphenyl) butane As shown in JP-A-63-151913 and JP-A-63-174009, a mixture with liquid paraffin and a mixture of phthalic acid esters with paraffin or polybutene are used. Also, as the immersion oil for microscopes for high refractive index, 1-bromonaphthalene or the like is generally used.
解像力は一般に次式で与えられる。 The resolving power is generally given by the following equation.
(λ=波長、N A=開口数、n=物体の屈折率、α=マ
ージナル光線の角度)式より、n、すなわち顕微鏡用
液浸油の屈折率が大きくなれば解像力が向上することが
わかる。 From the equation (λ = wavelength, NA = numerical aperture, n = refractive index of object, α = angle of marginal ray), it can be seen that the resolution increases as n, that is, the refractive index of the microscope immersion oil increases.
また、同じN A(開口数)の対物レンズを設計する上
で、高屈折率の顕微鏡用液浸油を用いると、先端対物レ
ンズに入射するマージナル光線の角度が小さくなるた
め、低屈折率の顕微鏡用液浸油を用いた場合より先端対
物レンズの形状等の設計が容易となる。Also, when designing an objective lens with the same NA (numerical aperture), the use of a high-refractive-index immersion oil for microscopes reduces the angle of the marginal ray incident on the front-end objective lens. The design of the shape of the tip objective lens and the like is easier than in the case of using liquid immersion oil.
しかし、特開昭63−15193号公報及び特開昭63−17400
9号公報で示されている顕微鏡用液浸油は、nD23が1.515
と小さいために、解像力が低く、かつ先端対物レンズ形
状等の設計を複雑なものにしている。However, JP-A-63-15193 and JP-A-63-17400
The immersion oil for microscopes disclosed in Japanese Patent Publication No. 9 has an nD 23 of 1.515.
Therefore, the resolution is low and the design of the shape of the tip objective lens is complicated.
一方、1−ブロムナフタリンは、nD23が1.657と高い
ために、解像力を高くすることができ、先端対物レンズ
形状等の設計も容易に行うことができるが、黄色に着色
しており、また人体に対して毒性がある。そして、プラ
スチック材料に対する溶解力が強いため、先端対物レン
ズにプラスチックレンズを用いた場合、あるいは対物レ
ンズ保管用のホルダー等がスチレン等凡用の熱可塑性プ
ラスチック材料であった場合は使用することができない
という問題があった。On the other hand, 1-bromo naphthalene is because nD 23 is high and 1.657, it is possible to increase the resolution, but also the design of such tip objective lens shape can be easily performed, were colored yellow, and the human body Toxic to. Since the dissolving power to the plastic material is strong, it cannot be used when a plastic lens is used as the tip objective lens, or when the holder for storing the objective lens is a general thermoplastic material such as styrene. There was a problem.
本発明は、かかる従来の問題点に鑑みてなされたもの
で、高屈折率で、高透明性を有しており、プラスチック
材料に対して溶解性がなく、また人体に対して低無毒性
である顕微鏡用液浸油を提供することを目的とする。The present invention has been made in view of such conventional problems, has a high refractive index, has high transparency, has no solubility in plastic materials, and has low non-toxicity to the human body. An object of the present invention is to provide a certain microscope immersion oil.
上記目的を達成するために、本発明は、 (R1,R2,R3,R4,R5,R6,R7およびR8は低級アルキル
基又はフェニル基)で表される化合物の中から選ばれた
少なくとも1種の屈折率が1.53〜1.65の範囲の化合物に
より顕微鏡用液浸油を構成した。In order to achieve the above object, the present invention provides (R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 and R 8 are lower alkyl groups or phenyl groups). Microscopic immersion oils were composed of compounds ranging from 1.53 to 1.65.
本発明において、上記構造式におけるnは1〜3が好
ましく、また低級アルキル基とは炭素数が5程度までの
ものが好ましい。高級アルキル基では、粘度が大きくな
る等の理由から適用することができない。In the present invention, n in the above structural formula is preferably 1 to 3, and the lower alkyl group preferably has up to about 5 carbon atoms. Higher alkyl groups cannot be applied due to reasons such as an increase in viscosity.
(第1実施例) 下記構造を持つメチルフェニルシリコーンを顕微鏡用
液浸油として用いた。(First Example) Methylphenyl silicone having the following structure was used as immersion oil for a microscope.
(テトラ・メチル・テトラフェニル・トリシロキサン) 上記の組成を有する顕微鏡用液浸油を用いて以下の評
価を行いその結果を表に示した。 (Tetra-methyl-tetraphenyl-trisiloxane) The following evaluation was performed using the microscope immersion oil having the above composition, and the results are shown in the table.
屈折率…JIS K2101に基づき(nD23),アッベ数(ν
D23)の測定を行った。Refractive index: Abbe number (ν) based on JIS K2101 (nD 23 )
D 23 ) was measured.
動粘度…JIS K2283に基づき25℃の動粘度測定を行っ
た。Kinematic viscosity: Kinematic viscosity was measured at 25 ° C based on JIS K2283.
不乾性…JIS K2201電気絶縁油に、蒸発量試験に基づ
き、30℃×24時間の試験を行い、蒸発量1wt%未満を良
好とした。Non-drying: A test was conducted on JIS K2201 electrical insulating oil at 30 ° C. for 24 hours based on the evaporation amount test, and an evaporation amount of less than 1 wt% was determined to be good.
外観 …試料を清浄なガラスビーカー内に取り、濁りの
有無を確認した。Appearance: The sample was placed in a clean glass beaker and checked for turbidity.
光照射試験 …一定量(40±0.5g)の試料をシャーレ(直径9c
m)に取り、光(光源として白色ランプ20Wを使用、ラン
プと試料との距離を15cmとした)を一定時間(24時間,1
20時間)照射後の屈折率の変化を測定した。Light irradiation test: Petri dish (diameter 9c) of a fixed amount (40 ± 0.5g)
m), and light (a white lamp of 20 W was used as a light source, and the distance between the lamp and the sample was 15 cm) for a certain period of time (24 hours, 1 hour)
(20 hours) The change in the refractive index after irradiation was measured.
加熱劣化試験 …一定量(40±0.5g)の試料を50mlの共栓付三角
フラスコに取り、一定温度(40℃,70℃)の恒温槽中で2
4時間放置し、その後の屈折率,アッベ数および色相の
変化を測定した。Heat deterioration test: A fixed amount (40 ± 0.5 g) of a sample is placed in a 50 ml stoppered Erlenmeyer flask and placed in a constant temperature bath (40 ° C, 70 ° C).
After allowing to stand for 4 hours, changes in refractive index, Abbe number and hue were measured.
耐食性…JIS K2501およびJIS K2400に基づき、全酸価,
塗抹標本用染料への影響を測定し、腐食性の有無を調べ
た。Corrosion resistance: Based on JIS K2501 and JIS K2400, total acid value,
The effect on the smear dye was measured to determine if it was corrosive.
コントラスト …得られた顕微鏡用液浸油を顕微鏡に投入して、
クロムを蒸着した白黒のプレート上に刻んだ白黒の線を
観察して、白黒の線の鮮明さを明瞭および不明瞭で評価
した。Contrast: Put the obtained microscope immersion oil into the microscope,
The sharpness of the black and white lines was evaluated as clear and unclear by observing the black and white lines cut on the black and white plate on which chromium was deposited.
解像力…屈折率により次の二段階で評価した。Resolution: Evaluated by the following two steps based on the refractive index.
良好 →1.558〜1.559 不良 →上記範囲外 透過率…JIS K0115に基づき測定を行い次の二段階で評
価した。Good → 1.558 to 1.559 Poor → Out of the above range Transmittance: Measured based on JIS K0115 and evaluated in the following two stages.
良好 →95%以上 不良 →95%以下 プラスチック溶解度 …厚さ0.5cm,1cm×1cm平板状のプラスチックが入
っている清浄なガラス容器に平板が満たされるように顕
微鏡用液浸油を入れ、24時間後、重量および外観表面形
状を観察した。なお、プラスチック材料はポリカーボネ
イト,ポリスチレン,アクリル樹脂,ポリプロピレンを
用い、いずれかのプラスチック材料に変化が生じた場合
を不良とした。Good → 95% or more Poor → 95% or less Plastic solubility… 0.5cm thick, 1cm x 1cm Fill a clean glass container with flat plastic with immersion oil for microscope to fill the flat plate for 24 hours Thereafter, the weight and external surface shape were observed. The plastic material used was polycarbonate, polystyrene, an acrylic resin, or polypropylene, and any change in the plastic material was regarded as defective.
なお、次表において本評価のそれぞれの項目において
良好なものを「○」とし不良なものを「×」として表記
した。In the following table, in each item of this evaluation, a good one was represented by “○”, and a poor one was represented by “×”.
また、同様の評価を比較例としてフタル酸エステル
類とパラフィンとの混合物、1−ブロムナフタリンに
ついて行い、同表に結果を併記した。Similar evaluations were performed for a mixture of phthalic esters and paraffin and 1-bromonaphthalene as comparative examples, and the results are shown in the same table.
本実施例の顕微鏡用液浸油は、屈折率が1.558と高
く、そのために解像力を向上することができる。そし
て、先端対物レンズの設計も容易になる。また、粘度が
40cStと小さいため、非常に作業性に優れている。さら
に、プラスチックに対する溶解性もなく、先端対物レン
ズおよび保管用ホルダーのプラスチック化を可能として
いる。The microscope immersion oil of the present embodiment has a high refractive index of 1.558, and therefore can improve the resolving power. And the design of the tip objective lens is also facilitated. Also, the viscosity is
Because it is as small as 40 cSt, it has excellent workability. Furthermore, it has no solubility in plastics, which makes the tip objective lens and the storage holder plastic.
(第2実施例) 下記構造を持つメチルフェニルシリコーンを顕微鏡用
液浸油として用いた。 Second Example Methylphenyl silicone having the following structure was used as immersion oil for a microscope.
(ペンタフェニル・トリメチル・トリシロキサン) 本実施例の顕微鏡用液浸油についても、第1実施例と
同様の評価を行った。解像力は、1.580〜1.581の範囲を
良好とする。 (Pentaphenyl / trimethyl / trisiloxane) The same evaluation as in the first example was performed for the microscope immersion oil of the present example. The resolution is preferably in the range of 1.580 to 1.581.
本実施例の顕微鏡用液浸油は、屈折率が1.580と非常
に高いため、解像力を非常に向上することができる。そ
して、先端対物レンズの設計も非常に容易になる。ま
た、粘度が175cStと小さいため、作業性に優れている。
さらに、プラスチックに対する溶解性もなく、先端対物
レンズおよび保管用ホルダーのプラスチック化を可能と
している。Since the microscope immersion oil of this embodiment has a very high refractive index of 1.580, the resolving power can be greatly improved. In addition, the design of the tip objective lens becomes very easy. Also, since the viscosity is as small as 175 cSt, it is excellent in workability.
Furthermore, it has no solubility in plastics, which makes the tip objective lens and the storage holder plastic.
なお、上記各実施例では、1種の化合物により液浸油
を構成したが、本発明はかかる実施例に限定されるもの
ではなく、2種以上の化合物を混合して構成してもよ
い。In each of the above embodiments, the liquid immersion oil is constituted by one kind of compound. However, the present invention is not limited to such an example, and may be constituted by mixing two or more kinds of compounds.
以上のように、本発明の顕微鏡用液浸油は、1.53〜1.
65の屈折率を有しており、そのために解像力を向上させ
ることができ、また先端対物レンズの設計も容易にする
ことができる。また、プラスチックに対する溶解性がな
く、レンズ、ホルダー等の液浸油が触れる部分のプラス
チック化を可能とした。更に、低粘度であり、作業性に
優れている。As described above, the immersion oil for a microscope of the present invention is 1.53 to 1.
It has a refractive index of 65, so that the resolving power can be improved and the design of the tip objective lens can be facilitated. In addition, since there is no solubility in plastic, it is possible to convert parts such as lenses and holders to be in contact with immersion oil into plastic. Furthermore, it has low viscosity and excellent workability.
Claims (1)
基又はフェニル基、nは1以上の整数)で表される化合
物の中から選ばれた少なくとも1種の化合物からなり、
屈折率が1.53〜1.65であることを特徴とする顕微鏡用液
浸油。(1) Wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 and R 8 are lower alkyl groups or phenyl groups, and n is an integer of 1 or more. Consisting of at least one compound,
An immersion oil for microscopes having a refractive index of 1.53 to 1.65.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2262276A JP2845287B2 (en) | 1990-09-28 | 1990-09-28 | Immersion oil for microscope |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2262276A JP2845287B2 (en) | 1990-09-28 | 1990-09-28 | Immersion oil for microscope |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH04138408A JPH04138408A (en) | 1992-05-12 |
JP2845287B2 true JP2845287B2 (en) | 1999-01-13 |
Family
ID=17373548
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2262276A Expired - Fee Related JP2845287B2 (en) | 1990-09-28 | 1990-09-28 | Immersion oil for microscope |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2845287B2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005340397A (en) * | 2004-05-25 | 2005-12-08 | Tokyo Ohka Kogyo Co Ltd | Immersion liquid for liquid immersion lithography process, and resist-pattern forming method using immersion liquid |
DE102009010503B4 (en) * | 2009-02-25 | 2017-02-16 | Carl Zeiss Ag | High-index immersion liquids and their use |
JP7052383B2 (en) * | 2018-01-30 | 2022-04-12 | 株式会社ニコン | Immersion for microscopes |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5510172A (en) * | 1978-07-10 | 1980-01-24 | Nippon Zeon Co | Rubber hose for fuel |
-
1990
- 1990-09-28 JP JP2262276A patent/JP2845287B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JPH04138408A (en) | 1992-05-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
LAPS | Cancellation because of no payment of annual fees |