JP2808227B2 - Inoculation method of liquorice mushroom - Google Patents
Inoculation method of liquorice mushroomInfo
- Publication number
- JP2808227B2 JP2808227B2 JP5241474A JP24147493A JP2808227B2 JP 2808227 B2 JP2808227 B2 JP 2808227B2 JP 5241474 A JP5241474 A JP 5241474A JP 24147493 A JP24147493 A JP 24147493A JP 2808227 B2 JP2808227 B2 JP 2808227B2
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- Japan
- Prior art keywords
- liquorice
- mushroom
- medium
- solid medium
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Description
【０００１】[0001]
【産業上の利用分野】本発明は、カンゾウタケの菌糸を
培養基に短期間で蔓延させることができるカンゾウタケ
種菌をカンゾウタケ用培養基に接種する方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for inoculating a culture medium for liquorice mushrooms with a liquorice mushroom species capable of spreading the hyphae of liquorice mushrooms on the culture medium in a short time.
【０００２】[0002]
【従来の技術】カンゾウタケ（Ｆｉｓｔｕｌｉｎａ ｈ
ｅｐａｔｉｃａ）は、ビフテキ茸とも呼ばれ、自然界で
は５〜６月または１０月頃、シイなどに発生する。その
子実体（傘）の形状は、肝臓状又は牛の舌状であり、表
面は赤紅色から暗赤褐色を呈する。また、その子実体の
大きさは直径１０〜２０ｃｍ程度である。このカンゾウ
タケは、子実体を薄く切り、生のまま、あるいはバター
等で炒めて食することができ、非常に美味なきのことし
て知られている。したがって、このカンゾウタケを栽培
し、年間を通して、多量に生産できれば、食生活を豊か
にし、また優れた加工食品を提供することができる。し
かし、近年、人工栽培によりきのこの生産量は高まって
いるが、その種類は限られており、カンゾウタケについ
ても人工栽培技術は確立していない。従来、カンゾウタ
ケの培養については、特公昭５２−４４６０３号公報及
び特公昭５４−２７９１２号公報に開示されているが、
これらの技術は、いずれもカンゾウタケの培養に使用し
た培地及びその培養によって得られた栄養菌糸体から坑
腫瘍性を有する物質を得ることを目的とするものであ
り、カンゾウタケの栄養菌糸体を多量に培養する技術で
あって、子実体を得ることを目的とするものではなく、
そのために培養基として液体培地を採用するものであっ
た。一方、固体培地を用いる場合には、きのこの種菌を
培地表面に接種するのが一般的であるが、カンゾウタケ
の場合、このように培地表面に接種すると菌糸の蔓延が
遅く、子実体を得るのに時間がかかることを見いだし
た。2. Description of the Related Art Licorice ( Fistulinah)
Epatica ) is also called beef mushroom and occurs in the wild in the natural world from May to June or around October. The shape of the fruiting body (umbrella) is liver-like or bovine tongue-like, and the surface exhibits a reddish red to a dark reddish brown. The size of the fruiting body is about 10 to 20 cm in diameter. This liquorice mushroom is known to be very tasteless because it can be cut into slices of the fruit body and eaten raw or fried with butter or the like. Therefore, if this liquorice mushroom is cultivated and can be produced in large quantities throughout the year, it is possible to enrich the diet and provide excellent processed foods. However, in recent years, the production amount of mushrooms has increased due to artificial cultivation, but the types thereof are limited, and no artificial cultivation technology has been established for liquorice mushrooms. Conventionally, the culture of liquorice mushrooms is disclosed in JP-B-52-44603 and JP-B-54-27912,
These techniques are intended to be from the vegetative mycelium obtained by medium and the culture was used either for the cultivation of beefsteak fungus obtaining a substance having an anti-neoplastic, a large amount of vegetative mycelium of beefsteak fungus It is a technique for culturing, not for obtaining fruiting bodies,
Therefore, a liquid medium was employed as a culture medium. On the other hand, when using a solid medium, it is common to inoculate a mushroom inoculum on the surface of the medium, but in the case of liquorice mushroom, inoculation on the surface of the medium in this way slows the spread of mycelia and produces fruiting bodies. To take time.
【０００３】[0003]
【発明が解決しようとする課題】本発明は、カンゾウタ
ケの人工栽培において短期間に菌糸を培養基に蔓延させ
ることができる、カンゾウタケの接種方法を提供するこ
とを目的とする。SUMMARY OF THE INVENTION An object of the present invention is to provide a method for inoculating liquorice mushrooms, which can spread hyphae into a culture medium in a short time in artificial cultivation of liquorice mushrooms.
【０００４】[0004]
【課題を解決するための手段】本発明は、きのこにおけ
る通常の接種方法とは異なり、固体培地全体に種菌を分
散させて接種した後、該培地を培養すると短期間にカン
ゾウタケの菌糸を培養基に蔓延させることができるとの
知見に基づいてなされたのである。すなわち、本発明
は、カンゾウタケの種菌を殺菌済カンゾウタケ用固体培
養基と混合することにより該種菌をカンゾウタケ用培養
基全体に分散させることを特徴とするカンゾウタケの接
種方法を提供する。本発明においては、任意の固体培地
を使用することかできるが、（ａ）木質原料と（ｂ）糖
類と有機体窒素を含む栄養物質とを含有し、ｐＨが3.5
〜5.5 の範囲にある固体培地を使用すると、より短期間
でカンゾウタケの菌糸を培養基に蔓延させることができ
るので、カンゾウタケの人工栽培の実用化を行う上で好
ましい。ここで、（ａ）の木質原料としては、例えば、
オガクズなどの木材の粉体を利用することが好ましい
が、その他にも樹木を粉砕機により粉砕、若しくは割砕
するか、あるいは鉋、スライサー等により薄片化したも
の等も利用できる。The present invention is different from the usual inoculation method in mushrooms in that the inoculation is carried out by dispersing a seed bacterium throughout a solid medium, and then culturing the medium, the mycelia of Licorice mushrooms are cultured in a short time. It was based on the finding that it could spread. That is, the present invention provides a method for inoculating liquorice mushrooms, which comprises dispersing the inoculum throughout the whole culture medium for liquorice mushrooms by mixing the inoculum of liquorice mushroom with a sterilized solid culture medium for liquorice mushrooms. In the present invention, any solid medium can be used, but it contains (a) a woody raw material, (b) a nutrient containing saccharides and organic nitrogen, and has a pH of 3.5.
The use of a solid medium in the range of 5.5 to 5.5 is preferable in that artificial hypha mushroom cultivation can be practically used because the mycelia of liquorice mushroom can be spread in the culture medium in a shorter period of time. Here, as the woody raw material (a), for example,
It is preferable to use wood powder such as sawdust, but it is also possible to use a material obtained by crushing or breaking a tree with a crusher, or flaked with a plane, a slicer, or the like.
【０００５】また、木質原料として用いる樹木は、ブ
ナ、シイ、コナラ、ミズナラ、ケヤキ、カシ、ハンノ
キ、シラカバ等の広葉樹、スギ、モミ、カラマツ、アカ
マツ、ヒノキ等の針葉樹のいずれであってもよいが、特
にブナ、シイ、コナラ、ミズナラが好ましい。なお、木
質原料は、目開き11.10mm の篩を通過でき、かつ目開き
850μｍの篩を通過し得ないもの、好ましくは目開き6.
00mmの篩を通過でき、かつ目開き1.00mmの篩を通過し得
ないものが、全体の50重量％以上、好ましくは70重量％
以上、さらに好ましくは80重量％以上を占めるものであ
る。より短期間でカンゾウタケの菌糸を培養基に蔓延さ
せるためである。[0005] The tree used as the woody raw material may be any of broad-leaved trees such as beech, sardine, oak, oak, zelkova, oak, alder, birch, etc., and conifers such as cedar, fir, larch, red pine, and hinoki. However, beech, shrimp, oak and oak are particularly preferred. The wood raw material can pass through a sieve with a mesh of 11.10 mm and
Those that cannot pass through a 850 μm sieve, preferably 6.
What can pass through a sieve of 00 mm and cannot pass through a sieve with an opening of 1.00 mm is 50% by weight or more, preferably 70% by weight of the whole
The content is more preferably 80% by weight or more. This is because the hypha of the liquorice mushroom spreads in the culture medium in a shorter time.
【０００６】（ｂ）の栄養物質は、糖類と有機体窒素と
を含むものである。この栄養物質として、グルコース、
ショ糖、デンプン、α化デンプン、マルツエキス等の糖
類、及びアミノ酸類、ペプトン、酵母エキス等の有機体
窒素を、それぞれについて１種又は２種以上を選択して
混合したものを使用できる。また、最初から糖類と有機
体窒素とを含む栄養物質として、例えば、トウモロコシ
の芯を粉砕して粒状又は粉状にしたコーンコブやコーン
ブランなどのトウモロコシ系の栄養添加物、オカラ、脱
脂大豆、大豆粉等の大豆系の栄養添加物、小麦胚芽、ラ
イ麦の全粒粉、カラス麦の全粒粉等の麦系等の栄養添加
物、米ヌカ、フスマ、麹などを挙げることができ、これ
らのなかでも、特に麹が好ましい。また、最初から糖類
と有機体窒素とを含む栄養物質に、糖類及び／又は有機
体窒素を加えて使用してもよい。なお、この栄養物質に
含まれる糖類及び有機体窒素の含量は、特に制限されな
いが、糖類は、栄養物質全体の０．１〜９０重量％、有
機体窒素は０．０２〜５０重量％であるのが好ましい。
また、前記木質原料と栄養物質との使用比率は、特に制
限されないが、乾燥重量として１：１〜９：１、好まし
くは３：１〜９：１となるようにする。なお、この固体
培地に、必要に応じてリン酸塩、マグネシウム塩、カル
シウム塩等の無機質及び金属塩類を添加しても良い。[0006] The nutrient (b) contains sugars and organic nitrogen. Glucose,
A saccharide such as sucrose, starch, pregelatinized starch, and malt extract, and an organic nitrogen such as amino acids, peptone, and yeast extract, each of which may be used alone or in combination of two or more, may be used. Also, as a nutrient containing sugars and organic nitrogen from the beginning, for example, corn cob or corn obtained by grinding corn core into granules or powder
Maize-based nutritional additives such as bran , okara, defatted soybeans, soybean-based nutritional additives such as soybean flour, wheat germ, rye whole wheat, oats whole wheat and other wheat-based nutritional additives, rice bran, Bran, koji and the like can be mentioned, and among these, koji is particularly preferred. Further, a saccharide and / or organic nitrogen may be added to a nutrient substance containing saccharide and organic nitrogen from the beginning. The content of the saccharide and the organic nitrogen contained in the nutrient is not particularly limited, but the saccharide is 0.1 to 90% by weight of the whole nutrient and the organic nitrogen is 0.02 to 50% by weight. Is preferred.
Further, the usage ratio of the woody raw material to the nutrient substance is not particularly limited, but is set to 1: 1 to 9: 1, preferably 3: 1 to 9: 1 as a dry weight. In addition, minerals and metal salts such as phosphates, magnesium salts, and calcium salts may be added to the solid medium as needed.
【０００７】前記木質原料と栄養物質とを含有する固体
培地の水分は、培地全体の重量に対して、５０〜７０重
量％、好ましくは５０〜６０重量％、特に好ましくは５
６〜５８重量％である。固体培地においては、加熱殺菌
した後のｐＨを、３．５〜５．５、好ましくは４〜５．
２、さらに好ましくは４．２〜５．０とする。培地のｐ
Ｈは、添加する栄養剤の種類や量により変化する。従っ
て、この培地のｐＨの調整は、例えば、加熱殺菌前又は
加熱殺菌後の培養基に、適当な無機酸及び有機酸、例え
ば、塩酸溶液、乳酸溶液、酢酸溶液、コハク酸緩衝液等
を添加することにより行う。なお、培地のｐＨ測定は、
培地５ｇを蒸留水５０ｍｌ中に懸濁させ、１０分間放置
した後にその懸濁液のｐＨを測定することにより行う。
本発明では、上記固体培地を常法により殺菌した後に
固体あるいは液体の種菌を上記固体培地に無菌的にほぼ
均一になるように混合し菌を固体培地中に分散せて培養
を行う。殺菌後の固体培地をクリーンベンチ等の無菌的
な環境下で種菌と無菌的に混合したあとで種菌がほぼ均
一になるように混合された固体培地を作成し培養を開始
する。あるいは、液体種菌等培地の隙間に流し込むこと
ができる形態の種菌を用いる場合は殺菌後の固体培地に
種菌を流し込むことにより、ほぼ均一に種菌が存在する
固体培地を作成し培養を開始してもよい。尚、固体培地
に対して任意の量の種菌を分散させることができるが、
好ましくは固体培地１００重量部あたり、０．４重量部
以上、好ましくは０．４〜２０重量部、より好ましくは
１〜１０重量部である。[0007] The water content of the solid medium containing the woody material and nutrients is 50 to 70% by weight, preferably 50 to 60% by weight, particularly preferably 5 to 60% by weight, based on the weight of the whole medium.
6 to 58% by weight. In a solid medium, the pH after heat sterilization is 3.5 to 5.5, preferably 4 to 5.5.
2, more preferably 4.2 to 5.0. Medium p
H changes depending on the type and amount of the nutrient added. Therefore, the pH of this medium is adjusted, for example, by adding an appropriate inorganic acid and organic acid, for example, a hydrochloric acid solution, a lactic acid solution, an acetic acid solution, a succinate buffer, etc. to the culture medium before or after heat sterilization. It is done by doing. In addition, pH measurement of the culture medium,
Suspending the media 5g of distilled water 50 ml, it performed more on measuring the pH of the suspension after standing for 10 minutes.
In the present invention, the solid medium is sterilized by a conventional method, and then a solid or liquid inoculum is aseptically mixed with the solid medium so as to be substantially uniform, and the bacteria are dispersed in the solid medium and cultured. After the sterilized solid medium is aseptically mixed with the inoculum under an aseptic environment such as a clean bench, a solid medium in which the inoculum is mixed so as to be substantially uniform is prepared and culture is started. Alternatively, in the case of using a seed in a form that can be poured into the space of the medium such as a liquid seed, by injecting the seed into the sterilized solid medium, even if a solid medium in which the seed is present is almost uniformly prepared and the culture is started. Good. In addition, any amount of inoculum can be dispersed in the solid medium,
Preferably, the amount is 0.4 parts by weight or more, preferably 0.4 to 20 parts by weight, more preferably 1 to 10 parts by weight, per 100 parts by weight of the solid medium.
【０００８】具体的には、固体培地を、所定の容器、好
ましくは、酸素や二酸化炭素等の気体は通すが、胞子等
の侵入を防止できるフィルター付き容器に充填する。続
いて、この培地を、例えば１００〜１２０°Ｃ、３０〜
３００分間の条件で加熱殺菌処理を施す。その後、殺菌
処理した固体培地に、無菌的に種菌を混合して、種菌が
固体培地に全体に万遍なく分散するようにして接種し、
この固体培地を、前記した酸素や二酸化炭素等の気体は
通すが、細菌の浸入を防止できるフィルター付き容器に
充填し、温度１０〜３０℃、好ましくは２０〜２５℃、
湿度５０〜９０％、好ましくは６０〜９０％、暗条件下
で培養する。種菌を接種した後、従来の方法によるより
も短期間で固体培地全体に菌糸が蔓延する。[0008] Specifically, the solid medium is filled in a predetermined container, preferably a container with a filter through which gas such as oxygen or carbon dioxide can pass but which can prevent invasion of spores and the like . Subsequently, the medium is heated at, for example, 100 to 120 ° C. and 30 to
Heat sterilization is performed under the condition of 300 minutes. Thereafter, the seed medium is aseptically mixed with the sterilized solid medium, and the seed medium is inoculated so as to be dispersed evenly throughout the solid medium.
This solid medium is passed through a gas such as oxygen or carbon dioxide as described above, but is filled in a container with a filter capable of preventing bacteria from entering, and the temperature is 10 to 30 ° C, preferably 20 to 25 ° C,
Culture is performed under a dark condition at a humidity of 50 to 90%, preferably 60 to 90%. After inoculation of the inoculum, the hypha spreads throughout the solid medium in a shorter time than with conventional methods.
【０００９】[0009]
【発明の効果】本発明によれば、短期間にカンゾウタケ
の菌糸を蔓延させ子実体発生基を得ることができる。
又、短期間に菌糸が蔓延するので子実体発生基の害菌に
対する抵抗性が増加し、カンゾウタケの培養を阻害する
害菌の増殖を減らすことができる。したがって、本発明
の接種方法は、実用的なカンゾウタケの人工栽培を行う
のに極めて適した方法である。次に、本発明の内容を実
施例により説明する。According to the present invention, it is possible to spread mycelia of liquorice in a short time to obtain a fruiting body generating group.
In addition, since hyphae spread in a short period of time, the resistance of the fruiting body generating group to harmful bacteria is increased, and the growth of harmful bacteria that inhibit cultivation of liquorice mushrooms can be reduced. Therefore, the inoculation method of the present invention is a method extremely suitable for carrying out artificial cultivation of liquorice mushrooms. Next, the contents of the present invention will be described with reference to examples.
【００１０】[0010]
【実施例】実施例１ 目開き２ｍｍの篩を通過せず、かつ目開き６ｍｍの篩を
通過するブナのチップ（全 体の１００重量％）５３４
ｇ及び乾燥麹１７８ｇに水分が６０重量％になるように
水を加えて、固体培地を得た。続いて、得られた固体培
地を２．５ｋｇ容量のフィルター付き、きのこ培養用パ
ウチ（商品名；キノパック、製造者；日昌（株））に密
度が０．５ｇ／ｃｍ３となるように充填し、これに１２
１℃、６０分間の条件で加熱殺菌処理をした。加熱殺菌
後の固体培地は、ｐＨ４．９であった。その後、無菌的
な条件下でカンゾウタケの種菌１７ｇ（上記固体培地と
同じ組成の培地に培養して得た種菌）を、該加熱殺菌し
た固体培地に加えて混合し、種菌を固体培地中に均一に
分散させた。この固体培地を、温度２５℃、湿度８５
％、暗所の条件下で２５日培養してカンゾウタケの菌糸
を培養基に蔓延させた。Example 1 Beech chips (100% by weight of the whole) 534 which do not pass through a sieve having an opening of 2 mm and pass through a sieve having an opening of 6 mm
g and 178 g of dried koji, water was added so that the water content was 60% by weight to obtain a solid medium. Subsequently, the obtained solid medium was filled into a mushroom culture pouch (trade name; Kinopack, manufacturer; Nissho Corporation) with a 2.5 kg capacity filter so as to have a density of 0.5 g / cm 3. And this has 12
Heat sterilization was performed at 1 ° C. for 60 minutes. The solid medium after heat sterilization had a pH of 4.9. Thereafter, 17 g of a liquorice mushroom inoculum (an inoculum obtained by culturing on a medium having the same composition as the solid medium) was added to the heat-sterilized solid medium under aseptic conditions and mixed, and the inoculum was homogenized in the solid medium. Was dispersed. This solid medium was heated at a temperature of 25 ° C. and a humidity of 85.
% Of the liquorice mushroom was spread on a culture medium for 25 days under dark conditions.
【００１１】比較例１ 実施例１に記載のブナのチップ７７５ｇ及び乾燥麹１７
８ｇを用いて、実施例１と同様にして固体培地を調製し
た（培地の水分含量 ６０％）この固体培地を２．５ｋ
ｇ容量のフィルター付き、きのこ培養用パウチ（商品
名； キノパック、製造者；日昌（株））に密度が０．
５ｇ／ｃｍ３となるように充填しこれを１２１℃、６０
分間の条件で加熱殺菌処理を施した。加熱殺菌後の固体
培地は、ｐＨ４．９であった。その後、無菌的な条件下
でカンゾウタケの種菌１７ｇ（上記固体培地と同じ組成
の培地に培養して得た種菌）を、該加熱殺菌した固体培
地の表面に接種した。この固体培地を、温度２５℃、湿
度８５％、暗所の条件下でカンゾウタケの菌糸が蔓延す
るまで培養したところ５５日間を要した。以上のよう
に、この方法によるとカンゾウタケの菌糸を培養基に蔓
延させるまでに延べ５５日かかり、実施例１のように２
５日以内にはカンゾウタケの菌糸を培養基に蔓延させる
ことができなかった。Comparative Example 1 775 g of the beech chips described in Example 1 and dried koji 17
Using 8 g, a solid medium was prepared in the same manner as in Example 1 (water content of the medium: 60%).
A mushroom culture pouch (trade name; Kinopack, manufacturer; Nissho Corporation) with a filter capacity of g capacity is 0.
5 g / cm 3 was charged at 121 ° C. and 60 ° C.
A heat sterilization treatment was performed under the conditions of minutes. The solid medium after heat sterilization had a pH of 4.9. Thereafter, 17 g of liquorice mushroom inoculum (inoculation obtained by culturing on a medium having the same composition as the solid medium) was inoculated on the surface of the heat-sterilized solid medium under aseptic conditions. This solid medium was cultured under conditions of a temperature of 25 ° C. and a humidity of 85% in a dark place until the hyphae of liquorice mushroom spread, and it took 55 days. As described above, according to this method, it takes a total of 55 days for the hyphae of the liquorice mushroom to spread on the culture medium.
Within 5 days, the mycelium of Licorice could not be spread on the culture medium.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭48−61240（ＪＰ，Ａ) 特開 昭63−17629（ＪＰ，Ａ) 特開 昭63−3730（ＪＰ，Ａ) 特開 平３−292816（ＪＰ，Ａ) 特公 昭47−38992（ＪＰ，Ｂ１) 特公 昭54−27912（ＪＰ，Ｂ１) 特公 昭52−44603（ＪＰ，Ｂ１) (58)調査した分野(Int.Cl.6，ＤＢ名) A01G 1/04──────────────────────────────────────────────────続 き Continuation of front page (56) References JP-A-48-61240 (JP, A) JP-A-63-17629 (JP, A) JP-A-63-3730 (JP, A) JP-A-3-3 292816 (JP, A) JP-B-47-38992 (JP, B1) JP-B-54-27912 (JP, B1) JP-B-52-44603 (JP, B1) (58) Fields investigated (Int. 6 , DB name) A01G 1/04
Claims (1)
菌済カンゾウタケ用固体培養基と混合することにより該
種菌をカンゾウタケ用培養基全体に分散させることを特
徴とするカンゾウタケの接種方法。1. A method for inoculating a liquorice mushroom, comprising mixing a seed of a liquorice mushroom with a solid culture medium for a killed liquorice mushroom containing a koji and dispersing the seed bacterium throughout the culture medium for a liquorice mushroom.
Priority Applications (12)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5241474A JP2808227B2 (en) | 1993-09-28 | 1993-09-28 | Inoculation method of liquorice mushroom |
| US08/305,353 US5590489A (en) | 1993-09-28 | 1994-09-13 | Method for growing fruit body of Fistulina hepatica |
| GB9502142A GB2284428B (en) | 1993-09-28 | 1994-09-16 | Method for mixing inocula of Fistulina hepatica with a sterilized solid culture medium |
| GB9418742A GB2282388B (en) | 1993-09-28 | 1994-09-16 | Method for growing fruit bodies of Fistulina hepatica |
| GB9502143A GB2284429B (en) | 1993-09-28 | 1994-09-16 | Method for forming fruit bodies of Fistulina hepatica |
| TW083108710A TW283622B (en) | 1993-09-28 | 1994-09-22 | |
| NL9401563A NL192716C (en) | 1993-09-28 | 1994-09-26 | Method of cultivation of fruit bodies of mushrooms. |
| CN94116581A CN1051202C (en) | 1993-09-28 | 1994-09-27 | Method for growing fruit body of fistulina hepatica |
| FR9411525A FR2710493B1 (en) | 1993-09-28 | 1994-09-27 | Process for developing the hat of Fistulina Hepatica. |
| KR1019940024568A KR0136838B1 (en) | 1993-09-28 | 1994-09-28 | Method of growing fruit body of fistulina hepatica |
| DE4434681A DE4434681A1 (en) | 1993-09-28 | 1994-09-28 | Process for growing fruiting bodies of Fistulina hepatica |
| NL9700003A NL9700003A (en) | 1993-09-28 | 1997-04-09 | Method of inoculating Fistulina hepatica |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5241474A JP2808227B2 (en) | 1993-09-28 | 1993-09-28 | Inoculation method of liquorice mushroom |
| US08/305,353 US5590489A (en) | 1993-09-28 | 1994-09-13 | Method for growing fruit body of Fistulina hepatica |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0795820A JPH0795820A (en) | 1995-04-11 |
| JP2808227B2 true JP2808227B2 (en) | 1998-10-08 |
Family
ID=26535281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5241474A Expired - Fee Related JP2808227B2 (en) | 1993-09-28 | 1993-09-28 | Inoculation method of liquorice mushroom |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2808227B2 (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5113697B2 (en) * | 1971-11-27 | 1976-05-01 | ||
| JPS5244603A (en) * | 1975-10-03 | 1977-04-07 | Matsushita Electric Ind Co Ltd | Automatic record player |
| DE2735000C3 (en) * | 1977-08-03 | 1980-05-08 | Siemens Ag, 1000 Berlin Und 8000 Muenchen | Diagnostic procedure for an electric starter motor |
| GB8613360D0 (en) * | 1986-06-03 | 1986-07-09 | Mycotech Ltd | Mushroom cultivation |
| JP2551937B2 (en) * | 1986-06-19 | 1996-11-06 | コ−ペクス エクスプロタル−アラポト クタト エス ジヤルト キツセオベトケゼト インノボコ−プ サクソポルト | Mushroom medium sterilization, inoculation, bagging method and equipment |
| JPH03292816A (en) * | 1990-04-12 | 1991-12-24 | Kazuki Ono | Method for culturing mushroom |
-
1993
- 1993-09-28 JP JP5241474A patent/JP2808227B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0795820A (en) | 1995-04-11 |
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