JP2805086B2 - Filler for living tissue - Google Patents
Filler for living tissueInfo
- Publication number
- JP2805086B2 JP2805086B2 JP1159307A JP15930789A JP2805086B2 JP 2805086 B2 JP2805086 B2 JP 2805086B2 JP 1159307 A JP1159307 A JP 1159307A JP 15930789 A JP15930789 A JP 15930789A JP 2805086 B2 JP2805086 B2 JP 2805086B2
- Authority
- JP
- Japan
- Prior art keywords
- filler
- tissue
- living tissue
- sponge
- months
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- Materials For Medical Uses (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は、損傷、欠損等の外科的治療、整形外科手術
等に使用される充填材に関する。Description: FIELD OF THE INVENTION The present invention relates to a filler used for surgical treatment of damage, defect and the like, orthopedic surgery and the like.
（従来技術） 損傷、欠損等の外科的治療、及び、整形外科手術等に
おいては組織の再生、拘縮を防止する目的において、欠
損部に充填材が埋入される。(Prior Art) In a surgical treatment of injury, defect, or the like, and in an orthopedic surgery, a filler is inserted into a defect for the purpose of preventing tissue regeneration and contracture.
かかる素材としては、組織反応が少なく、線維芽細胞
の増殖を促し、組織が再生するまで長期にわたってその
強度、形状が維持される機能が求められる。また、特に
適用中においては組織の拘縮を防止する目的において保
形性を有する機能が求められ、また、組織の再生後にお
いては異物として体内に残留することなく速やかに消失
することが理想とされる。Such a material is required to have a function of reducing tissue reaction, promoting the proliferation of fibroblasts, and maintaining its strength and shape for a long period until the tissue is regenerated. In addition, especially during application, a function having shape retention properties is required for the purpose of preventing contracture of the tissue, and after regeneration of the tissue, it is ideal that the tissue disappears quickly without remaining as a foreign substance in the body. Is done.
かかる目的に対し、ミクロポーラスなコラーゲンスポ
ンジが提案されているが、上記の機能を満足しない。For this purpose, a microporous collagen sponge has been proposed, but does not satisfy the above functions.
（発明が解決しようとする問題点） 即ち、例えば、グルタールアルデヒドを用いて架橋さ
せたコラーゲンスポンジは、生体に埋入後２〜３ヶ月後
には完全に生体に分解吸収されて消失してしまい治癒に
必要な長期の強度、保形性を維持しない。(Problems to be Solved by the Invention) That is, for example, a collagen sponge cross-linked using glutaraldehyde is completely decomposed and absorbed by a living body two to three months after implantation into the living body and disappears. Does not maintain the long-term strength and shape retention required for healing.
本発明は、かかる従来の欠点を解消し、組織反応が少
なく、且つ、線維芽細胞の増殖を促すと共に、長期にわ
たって形状、強度が維持され、また治癒後は生体に吸収
される新規な充填材を提供したものである。The present invention solves the conventional drawbacks, reduces tissue reactions, promotes fibroblast proliferation, maintains shape and strength for a long time, and is a novel filler that is absorbed by a living body after healing. Is provided.
（問題を解決するための手段） しかるに、本発明はコラーゲンスポンジと生体分解吸
収性高分子材料との複合材料より成り、かかる生体分解
吸収性高分子材料として繊維状のポリ−Ｌ−乳酸を用い
たこと、およびこれをコラーゲンスポンジ中に混在、も
しくは埋入させて構成したことに特徴を有するものであ
る。(Means for Solving the Problem) However, the present invention comprises a composite material of a collagen sponge and a biodegradable and absorbable polymer material, and uses fibrous poly-L-lactic acid as the biodegradable and absorbable polymer material. And that they are mixed or embedded in a collagen sponge.
（作用） 本発明は、コラーゲンスポンジ中に生体内での分解速
度の遅いポリ−Ｌ−乳酸を混在させて複合化させたこと
によってスポンジ構造のporeを長期にわたって維持で
き、また、繊維状のポリ−Ｌ−乳酸との複合化によって
内部への線維芽細胞の増殖を促すと共に、治癒に必要な
長期にわたっての強度、形状の維持を可能としたもので
ある。(Action) The present invention can maintain a sponge-structured pore over a long period of time by mixing and mixing poly-L-lactic acid, which has a low decomposition rate in a living body, into a collagen sponge. In addition to promoting the growth of fibroblasts inside by complexing with -L-lactic acid, it is possible to maintain the strength and shape for a long time necessary for healing.
以下、その構成について、例示する。 Hereinafter, the configuration will be exemplified.
（構成例） ３デニールのポリ−Ｌ−乳酸糸（分子量80000）0.3g
をからめてスライバー状とし、これを縦、横、深さが夫
々６×２×2cmの容器に入れ、これに豚由来のアテロコ
ラーゲン0.3％塩酸溶液50gを1800rpmにて60分間撹拌し
て注いだ。次いで、これを48時間凍結乾燥し、アルコー
ルにて滅菌して本発明充填材を構成した。(Structural example) 0.3 g of 3-denier poly-L-lactic acid yarn (molecular weight: 80000)
The mixture was put into a sliver shape, placed in a container having a length, width and depth of 6 × 2 × 2 cm, and 50 g of a pig-derived atelocollagen 0.3% hydrochloric acid solution was stirred at 1800 rpm for 60 minutes and poured. Next, this was freeze-dried for 48 hours and sterilized with alcohol to constitute the filler of the present invention.
このようにして得た充填材は、ミクロポーラスなスポ
ンジ構造の間にポリ−Ｌ−乳酸糸がランダムに埋入され
て複合化された外観を呈した。The filler thus obtained exhibited a composite appearance in which poly-L-lactic acid yarns were randomly embedded between microporous sponge structures.
また、その物性値は第１表に示すように従来のコラー
ゲン単独のスポンジと比較し、破断強度、破断伸度、ヤ
ング率が格段に高い値を示し、著しい改善が成された。
また、PoreSizeも大きくなっている。In addition, as shown in Table 1, the physical properties of the collagen sponge were significantly higher than those of a conventional sponge made of collagen alone, and the breaking strength, elongation at break, and Young's modulus were remarkably high.
Also, PoreSize is getting bigger.
尚、表における比較例は架橋剤としてグルタールアル
デヒドを使用した豚由来のアテロコラーゲン0.2％塩酸
溶液50gを前記と同様の方法によって処理して得たコラ
ーゲン単独のスポンジである。The comparative example in the table is a collagen-only sponge obtained by treating 50 g of a pig-derived atelocollagen 0.2% hydrochloric acid solution using glutaraldehyde as a crosslinking agent in the same manner as described above.
尚、これの測定はJIS法に準じた。また、その単位は
以下の通りである。 In addition, this measurement followed the JIS method. The unit is as follows.
強度：破断強度（×105），（dyne/cm2） 伸度：破断伸度（％） ヤング率：（×105），（dyne/cm2） PoreSize:（μｍ） 上記の方法により得た本発明充填材を以下の方法によ
り動物実験に供し、組織学的検討と拘縮の状態を観察し
た。Strength: breaking strength (× 10 5 ), (dyne / cm 2 ) Elongation: breaking elongation (%) Young's modulus: (× 10 5 ), (dyne / cm 2 ) PoreSize: (μm) Obtained by the above method The packing material of the present invention was subjected to animal experiments by the following method, and histological examination and the state of contracture were observed.
（適用例） 体重350gのウィスター系ラットの背部筋層上を２×2c
m大に剥離し、その部分に約2cm3大の本発明充填材を埋
植し、経過を観察した。(Application example) 2 × 2c on the back muscle layer of a Wistar rat weighing 350 g
The sample was peeled to a size of m, and about 2 cm 3 of the filler of the present invention was implanted in that portion, and the progress was observed.
＜１ヶ月後＞ スポンジ内周部分で線維芽細胞の侵入が認められるが
中央部では細胞未侵入。<One month later> Infiltration of fibroblasts was observed in the inner periphery of the sponge, but no cells invaded in the center.
＜３ヶ月後＞ スポンジ中央部への細胞侵入は２ヶ月後に比べて増加
している。<After 3 months> Cell invasion into the center of the sponge has increased compared to 2 months later.
＜６ヶ月後＞ スポンジ中央部へ線維芽細胞が一定の方向で並ぶ部分
ができた。<After 6 months> There was a portion where fibroblasts were arranged in a certain direction in the center of the sponge.
組織学的検討において埋植３〜４ヶ月後にスポンジの
中央部まで線維芽細胞が十分に侵入し、６ヶ月後におい
ては完全に組織が構築された。In histological examination, fibroblasts sufficiently penetrated to the center of the sponge 3 to 4 months after implantation, and the tissue was completely constructed after 6 months.
一方、拘縮の状態については石膏模型により、その容
積を測定する方法によって行なったが、上記比較例によ
るものは２ヶ月後で初期体積の５〜15％しか残存せず、
４ヶ月後では殆ど生体内に吸収され、消失するという結
果であったが、本発明充填材によると６ヶ月後において
も初期体積の35〜50％が残存し、かかる面においても顕
著な差が見られた。On the other hand, the condition of the contracture was performed by a method of measuring the volume by a plaster model, but in the case of the comparative example, only 5 to 15% of the initial volume remained after 2 months,
After 4 months, it was almost absorbed into the living body and disappeared. However, according to the filler of the present invention, 35 to 50% of the initial volume remained even after 6 months, and there was a remarkable difference in this aspect. Was seen.
（発明の効果） 以上のように本発明による充填材は、その実用結果か
らも明らかなように、用途上の要求特性である、組織反
応がないこと、線維芽細胞の増殖を促すこと、組織が再
生するまで長期にわたってその強度、形状が維持される
こと、組織の拘縮を防止する機能を有すこと、組織の再
生後は体内に分解吸収されて消失してしまうこと等、こ
の種の用途に必要な機能を全て兼ね備えたものであり、
効果的な適用が可能なものである。(Effects of the Invention) As described above, the filler according to the present invention, as apparent from its practical results, has properties required for use such as no tissue reaction, promotion of fibroblast proliferation, The strength and shape are maintained for a long time until the tissue is regenerated, it has the function to prevent contracture of the tissue, and after the tissue is regenerated, it is decomposed and absorbed into the body and disappears. It has all the functions necessary for the application,
It can be applied effectively.
尚、コラーゲンスポンジと生体分解吸収性高分子材料
との複合化率、およびポリ−Ｌ−乳酸繊維の繊維度はそ
の用途、必要機能等に応じて任意に選択可能なものであ
る。The composite ratio of the collagen sponge and the biodegradable and absorbable polymer material and the degree of fiber of the poly-L-lactic acid fiber can be arbitrarily selected according to the use, the required function, and the like.
以上のように本発明は、従来にない新規な構成の生体
組織用充填材を提供したものである。As described above, the present invention provides a filler for living tissue having a novel structure which has not been provided in the past.
フロントページの続き (56)参考文献 特開 昭55−53214（ＪＰ，Ａ) 特開 昭58−41559（ＪＰ，Ａ) 特開 昭60−261460（ＪＰ，Ａ) (58)調査した分野(Int.Cl.6，ＤＢ名) A61L 27/00Continuation of the front page (56) References JP-A-55-53214 (JP, A) JP-A-58-41559 (JP, A) JP-A-60-261460 (JP, A) (58) Fields investigated (Int) .Cl. 6 , DB name) A61L 27/00
Claims (3)
子より成る繊維素材との複合物より成ることを特徴とす
る生体組織用充填材。1. A filler for living tissue, comprising a composite of a collagen sponge and a fiber material comprising a biodegradable and absorbable polymer.
ーゲンスポンジ中に混在し、もしくは埋入されて成るこ
とを特徴とする請求項（１）項記載の生体組織用充填
材。2. The filler for living tissue according to claim 1, wherein the fibrous biodegradable and absorbable polymer material is mixed or embedded in a collagen sponge.
酸であることを特徴とする請求項（１）、（２）項記載
の生体組織用充填材。3. The filler for living tissue according to claim 1, wherein the biodegradable and absorbable polymer material is poly-L-lactic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1159307A JP2805086B2 (en) | 1989-06-20 | 1989-06-20 | Filler for living tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1159307A JP2805086B2 (en) | 1989-06-20 | 1989-06-20 | Filler for living tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0323864A JPH0323864A (en) | 1991-01-31 |
| JP2805086B2 true JP2805086B2 (en) | 1998-09-30 |
Family
ID=15690945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1159307A Expired - Lifetime JP2805086B2 (en) | 1989-06-20 | 1989-06-20 | Filler for living tissue |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2805086B2 (en) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9206509D0 (en) * | 1992-03-25 | 1992-05-06 | Jevco Ltd | Heteromorphic sponges containing active agents |
| US5565210A (en) * | 1993-03-22 | 1996-10-15 | Johnson & Johnson Medical, Inc. | Bioabsorbable wound implant materials |
| JPH08257045A (en) * | 1993-05-12 | 1996-10-08 | Shuji Ichinohe | Sponge belt resuscitation reaction |
| GB2281861B (en) * | 1993-09-21 | 1997-08-20 | Johnson & Johnson Medical | Bioabsorbable wound implant materials containing microspheres |
| CA2175049A1 (en) * | 1993-10-28 | 1995-05-04 | Timothy Ringeisen | Improved process and device for treating and healing a bone void |
| US5981825A (en) | 1994-05-13 | 1999-11-09 | Thm Biomedical, Inc. | Device and methods for in vivo culturing of diverse tissue cells |
| TW369414B (en) * | 1994-09-30 | 1999-09-11 | Yamanouchi Pharma Co Ltd | Bone formation transplant |
| JP2778510B2 (en) * | 1995-03-09 | 1998-07-23 | 日産自動車株式会社 | Ultrasonic thickness measurement sensor |
| JP2010029684A (en) * | 1998-03-06 | 2010-02-12 | Yoshihiko Shimizu | Collagen material and process for producing the same |
| EP1053758A1 (en) * | 1999-05-19 | 2000-11-22 | Resorba Chirurgisches Nahtmaterial Franz Hiltner GmbH & Co. | Bioabsorbable implant |
| JP3603179B2 (en) | 1999-09-09 | 2004-12-22 | グンゼ株式会社 | Cardiovascular tissue culture substrate and tissue regeneration method |
| KR20020062301A (en) | 1999-11-09 | 2002-07-25 | 덴끼 가가꾸 고교 가부시키가이샤 | Use of soluble cellulose derivative having been made hardly soluble in water and process for producing the same |
| GB0006439D0 (en) * | 2000-03-18 | 2000-05-10 | Univ Nottingham | Polymeric composite materials and their manufacture |
| CA2425935C (en) | 2000-11-07 | 2011-03-29 | Cryolife, Inc. | Expandable foam-like biomaterials and methods |
| EP1433487A1 (en) * | 2001-08-22 | 2004-06-30 | National Institute of Advanced Industrial Science and Technology | Method of bone regeneration |
| US20030059460A1 (en) * | 2001-09-27 | 2003-03-27 | Yasuhiko Tabata | Hybrid material for regeneration of living body tissue |
| KR20030032420A (en) * | 2001-10-18 | 2003-04-26 | 한국과학기술연구원 | Porous Scaffold Made of Biodegradable Polymer for Reconstructing Damaged Ocular Tissue |
| JP2005087641A (en) * | 2003-09-19 | 2005-04-07 | Jms Co Ltd | Bio-absorbent high polymer film |
| US20080176206A1 (en) | 2007-01-18 | 2008-07-24 | Toshiharu Shinoka | Cardiovascular tissue culture substrate |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2843963A1 (en) * | 1978-10-09 | 1980-04-24 | Merck Patent Gmbh | BODY-RESORBABLE SHAPED MATERIAL BASED ON COLLAGEN AND THEIR USE IN MEDICINE |
| DE3037513C2 (en) * | 1980-10-03 | 1983-05-05 | Steffan, Wolfgang, 8425 Neustadt | Collagen wound dressing |
| JPS60261460A (en) * | 1984-06-11 | 1985-12-24 | 株式会社 高研 | Artifical skin comprising collagen and poly-alpha-amino acidmembrane |
-
1989
- 1989-06-20 JP JP1159307A patent/JP2805086B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0323864A (en) | 1991-01-31 |
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