JP2732840B2 - ヒトサイトメガロウィルス構造燐タンパク質、その製造および使用 - Google Patents
ヒトサイトメガロウィルス構造燐タンパク質、その製造および使用Info
- Publication number
- JP2732840B2 JP2732840B2 JP62145450A JP14545087A JP2732840B2 JP 2732840 B2 JP2732840 B2 JP 2732840B2 JP 62145450 A JP62145450 A JP 62145450A JP 14545087 A JP14545087 A JP 14545087A JP 2732840 B2 JP2732840 B2 JP 2732840B2
- Authority
- JP
- Japan
- Prior art keywords
- hcmv
- human cytomegalovirus
- genome
- protein
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000701024 Human betaherpesvirus 5 Species 0.000 title claims abstract description 25
- 102000007982 Phosphoproteins Human genes 0.000 title claims description 6
- 108010089430 Phosphoproteins Proteins 0.000 title claims description 6
- 238000004519 manufacturing process Methods 0.000 title description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 32
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 20
- 239000012634 fragment Substances 0.000 claims abstract description 15
- 230000002163 immunogen Effects 0.000 claims abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 6
- 238000002965 ELISA Methods 0.000 claims abstract description 3
- 229960005486 vaccine Drugs 0.000 claims abstract 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 6
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 claims description 2
- 101710172711 Structural protein Proteins 0.000 abstract description 3
- 238000010353 genetic engineering Methods 0.000 abstract description 2
- 239000002299 complementary DNA Substances 0.000 description 8
- 241000283973 Oryctolagus cuniculus Species 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 108020001507 fusion proteins Proteins 0.000 description 6
- 102000037865 fusion proteins Human genes 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 108020005202 Viral DNA Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 102220201851 rs143406017 Human genes 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 1
- 206010062343 Congenital infection Diseases 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 210000003953 foreskin Anatomy 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Communicable Diseases (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3619718 | 1986-06-12 | ||
| DE3619718.1 | 1986-06-12 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20729497A Division JP3272641B2 (ja) | 1986-06-12 | 1997-07-16 | ヒトサイトメガロウィルス構造燐タンパク質の免疫原性部分、その製造および使用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62296893A JPS62296893A (ja) | 1987-12-24 |
| JP2732840B2 true JP2732840B2 (ja) | 1998-03-30 |
Family
ID=6302829
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62145450A Expired - Lifetime JP2732840B2 (ja) | 1986-06-12 | 1987-06-12 | ヒトサイトメガロウィルス構造燐タンパク質、その製造および使用 |
| JP20729497A Expired - Lifetime JP3272641B2 (ja) | 1986-06-12 | 1997-07-16 | ヒトサイトメガロウィルス構造燐タンパク質の免疫原性部分、その製造および使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20729497A Expired - Lifetime JP3272641B2 (ja) | 1986-06-12 | 1997-07-16 | ヒトサイトメガロウィルス構造燐タンパク質の免疫原性部分、その製造および使用 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US5728578A (enExample) |
| EP (1) | EP0252531B1 (enExample) |
| JP (2) | JP2732840B2 (enExample) |
| AT (1) | ATE82985T1 (enExample) |
| AU (1) | AU605155B2 (enExample) |
| DE (1) | DE3782867D1 (enExample) |
| ES (1) | ES2044881T3 (enExample) |
| GR (1) | GR3007031T3 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE132533T1 (de) * | 1988-02-24 | 1996-01-15 | Behringwerke Ag | Strukturelles phosphoprotein (pp28) des menschlichen cytomegalovirus (hcmv), seine herstellung und verwendung |
| US5591439A (en) * | 1989-03-24 | 1997-01-07 | The Wistar Institute Of Anatomy And Biology | Recombinant cytomegalovirus vaccine |
| DK0389286T3 (da) * | 1989-03-24 | 1996-11-18 | Wistar Inst | Rekombinant-cytomegalovirus-vaccine |
| US5552143A (en) * | 1989-03-24 | 1996-09-03 | The Wistar Institute Of Anatomy & Biology | Recombinant cytomegalovirus vaccine |
| GB9014652D0 (en) * | 1990-07-02 | 1990-08-22 | Wellcome Found | Antigen |
| DE4128684A1 (de) * | 1991-08-29 | 1993-03-04 | Behringwerke Ag | Hcmv-spezifische peptide, mittel dazu und ihre verwendung |
| US5744298A (en) * | 1991-08-29 | 1998-04-28 | Behringwerke Aktiengesellschaft | DNA sequences which encode immunoreactive HCMV-specific peptides |
| AU2809897A (en) * | 1996-04-23 | 1997-11-12 | Wistar Institute Of Anatomy And Biology, The | Novel human cytomegalovirus dna constructs and uses therefor |
| DE60109633T2 (de) * | 2000-10-20 | 2006-01-19 | City Of Hope, Duarte | Immunreaktive peptidische ctl-epitope aus dem menschlichen cytomegalovirus pp150 |
| US20040228842A1 (en) * | 2003-02-27 | 2004-11-18 | Shan Lu | Compositions and methods for cytomegalovirus treatment |
| GB0408164D0 (en) | 2004-04-13 | 2004-05-19 | Immune Targeting Systems Ltd | Antigen delivery vectors and constructs |
| GB0716992D0 (en) * | 2007-08-31 | 2007-10-10 | Immune Targeting Systems Its L | Influenza antigen delivery vectors and constructs |
| BR112012015523A2 (pt) | 2009-12-23 | 2017-04-18 | 4-Antibody Ag | membros de ligação do citomegalovirus humano, composição contendo os mesmos, método de tratamento, moléculas de anticorpo, método de produção, molecular de ácido nucléico, célula hospedeira, e, método de neutralização do hcmv. |
| CN104569387B (zh) * | 2015-01-13 | 2017-06-16 | 必欧瀚生物技术（合肥）有限公司 | 一种ELISA检测人血清HCMV pp150 IgM抗体试剂盒 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554101A (en) * | 1981-01-09 | 1985-11-19 | New York Blood Center, Inc. | Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity |
| US4769331A (en) * | 1981-09-16 | 1988-09-06 | University Patents, Inc. | Recombinant methods and materials |
| US4554159A (en) * | 1981-11-12 | 1985-11-19 | Institute Merieux | Vaccine and method of immunizing against herpes simplex virus (types 1 and 2) |
| US4762780A (en) * | 1984-04-17 | 1988-08-09 | The Regents Of The University Of California | Method and composition for screening and diagnosing "HCMV" |
| GB8404368D0 (en) * | 1984-02-20 | 1984-03-28 | Cogent Ltd | Monoclonal antibodies to human cytomegalovirus |
| US4743562A (en) * | 1984-08-21 | 1988-05-10 | The Board Of Trustees Of The Leland Stanford Junior University | Purified human cytomegalovirus protein |
| US4689225A (en) * | 1984-11-02 | 1987-08-25 | Institut Merieux | Vaccine for cytomegalovirus |
| US4808518A (en) * | 1985-02-11 | 1989-02-28 | University Of Tennessee Research Corporation | Recovery of cytomegalovirus antigen and use thereof in an assay |
-
1987
- 1987-06-05 AT AT87111726T patent/ATE82985T1/de not_active IP Right Cessation
- 1987-06-05 EP EP87111726A patent/EP0252531B1/de not_active Expired - Lifetime
- 1987-06-05 ES ES87111726T patent/ES2044881T3/es not_active Expired - Lifetime
- 1987-06-05 DE DE87111726T patent/DE3782867D1/de not_active Expired - Lifetime
- 1987-06-11 AU AU74128/87A patent/AU605155B2/en not_active Expired
- 1987-06-12 JP JP62145450A patent/JP2732840B2/ja not_active Expired - Lifetime
-
1993
- 1993-02-11 GR GR920402953T patent/GR3007031T3/el unknown
- 1993-12-01 US US08/159,710 patent/US5728578A/en not_active Expired - Lifetime
-
1995
- 1995-04-28 US US08/430,274 patent/US5846733A/en not_active Expired - Lifetime
-
1997
- 1997-07-16 JP JP20729497A patent/JP3272641B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62296893A (ja) | 1987-12-24 |
| US5846733A (en) | 1998-12-08 |
| AU605155B2 (en) | 1991-01-10 |
| DE3782867D1 (en) | 1993-01-14 |
| ATE82985T1 (de) | 1992-12-15 |
| AU7412887A (en) | 1987-12-17 |
| EP0252531B1 (de) | 1992-12-02 |
| ES2044881T3 (es) | 1994-01-16 |
| JP3272641B2 (ja) | 2002-04-08 |
| EP0252531A1 (de) | 1988-01-13 |
| JPH10113190A (ja) | 1998-05-06 |
| US5728578A (en) | 1998-03-17 |
| GR3007031T3 (enExample) | 1993-07-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
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| R250 | Receipt of annual fees |
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| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (event date is renewal date of database) |
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