JP2729883B2 - Method for producing organic secretions using cyanobacteria - Google Patents
Method for producing organic secretions using cyanobacteriaInfo
- Publication number
- JP2729883B2 JP2729883B2 JP4268960A JP26896092A JP2729883B2 JP 2729883 B2 JP2729883 B2 JP 2729883B2 JP 4268960 A JP4268960 A JP 4268960A JP 26896092 A JP26896092 A JP 26896092A JP 2729883 B2 JP2729883 B2 JP 2729883B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- culture solution
- carbon source
- cyanobacteria
- organic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、藍藻の培養液から有機
酸および（または）アルコールを取得する、藍藻を用い
る有機分泌物の製造方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing an organic secretion using a blue-green algae for obtaining an organic acid and / or alcohol from a culture solution of the blue-green algae.
【０００２】[0002]
【従来技術および発明の課題】微生物を使用した有機酸
および（または）アルコールの製造方法については、従
来、多数の報告がある。例えば、特開昭６４−６７１８
０および特開昭６３−８７９８５ではエタノールの製造
方法が、また特開昭５９−１７９０８９では酢酸の製造
方法が提案されている。しかし、これらは何れの場合も
炭素源としてグルコース等の有機炭素源を必要としてい
る。2. Description of the Related Art There have been many reports on methods for producing organic acids and / or alcohols using microorganisms. For example, JP-A-64-6718
0 and JP-A-63-87985 propose a method for producing ethanol, and JP-A-59-179089 proposes a method for producing acetic acid. However, these require an organic carbon source such as glucose as a carbon source in any case.
【０００３】また、特殊な微生物を利用して有機炭素源
を必要としないで有機酸およびアルコールを製造する方
法も提案されている。例えば、特開昭５９−１７９０８
８はアセトバクテリウム属に属する新規な細菌を用い
て、二酸化炭素から酢酸を製造する方法を開示してい
る。しかし、この方法では、酢酸生産に二酸化炭素と同
時にＨ2 等の還元物質が必須となっている。[0003] Further, there has been proposed a method for producing organic acids and alcohols using a special microorganism without requiring an organic carbon source. For example, see JP-A-59-17908.
No. 8 discloses a method for producing acetic acid from carbon dioxide using a novel bacterium belonging to the genus Acetobacterium. However, in this method, a reducing substance such as H 2 is required at the same time as carbon dioxide for acetic acid production.
【０００４】さらに、例えば、Bioscience, Biotechnol
ogy and Biochemistry,Vol.56, No.5,751-754(1992) で
は、炭素源として無機炭素源のみを用いて緑藻を光照射
下で培養し、さらに暗嫌気条件下で培養することによ
り、酢酸およびエタノールを得る方法が報告されてい
る。しかし、この方法では、酢酸およびエタノールの生
産量は極めて低く、実用化は期待できない。Further, for example, Bioscience, Biotechnol
ogy and Biochemistry, Vol. 56, No. 5, 751-754 (1992), cultivation of green algae under light irradiation using only an inorganic carbon source as a carbon source, and further cultivation under dark anaerobic conditions, Methods for obtaining ethanol have been reported. However, in this method, the production amounts of acetic acid and ethanol are extremely low, and commercialization cannot be expected.
【０００５】本発明の課題は、上記のような実情から、
有機炭素源やＨ2 等の還元物質を用いる必要がなく、藍
藻の培養により無機炭素源から有機酸および（または）
アルコールを高収率で取得する方法を提供する点にあ
る。[0005] The object of the present invention is to solve the above situation,
There is no need to use organic carbon sources or reducing substances such as H 2, and organic acids and / or
An object of the present invention is to provide a method for obtaining an alcohol at a high yield.
【０００６】[0006]
【課題を解決するための手段】本発明者等は、上記課題
を解決すべく鋭意研究の結果、藍藻を光照射下で、炭酸
ガス、重炭酸金属塩などの無機炭素源を用いて培養し、
その培養物を暗嫌気条件下で培養することにより培養液
から有機酸および（または）アルコールを取得できるこ
とを見い出し、本発明を完成するに至った。Means for Solving the Problems The inventors of the present invention have conducted intensive studies to solve the above-mentioned problems, and as a result, the blue-green algae were cultured under light irradiation using an inorganic carbon source such as carbon dioxide and metal bicarbonate. ,
The present inventors have found that an organic acid and / or an alcohol can be obtained from a culture solution by culturing the culture under dark anaerobic conditions, thereby completing the present invention.
【０００７】すなわち、本発明によれば、藍藻を光照射
下で、炭素源として無機炭素源を用いて培養し、その培
養物をさらに暗嫌気条件下で培養することにより、有機
酸および（または）アルコールを取得する、藍藻を用い
る有機分泌物の製造方法が提供せられる。That is, according to the present invention, a cyanobacterium is cultured under light irradiation using an inorganic carbon source as a carbon source, and the culture is further cultured under dark anaerobic conditions, whereby organic acids and (or A) A method for producing an organic secretion using a cyanobacterium to obtain an alcohol is provided.
【０００８】以下、本発明を詳しく説明する。Hereinafter, the present invention will be described in detail.
【０００９】ａ） 藍藻を光照射下で無機炭素源を用い
て培養するに当たり、使用する藍藻は、暗嫌気条件下で
培養液中に有機酸および（または）アルコールを放出す
る藍藻であればいかなるものでもよい。好適に用いられ
る藍藻としては、例えばスピルリナ・プラテンシス（Sp
irulina platensis ）財団法人 地球・人間環境フォー
ラム（以下ＧＥＦと記す）寄託NIES-39 、スピルリナ・
プラテンシス（Spirulina platensis ）ＧＥＦ寄託NIES
-46 、オシラトリア・リムネチカ（Oscillatoria limne
tica）ＧＥＦ寄託NIES-36 、ミクロシスティス・アエル
ギノーサ（Microcystis aeruginosa) ＧＥＦ寄託NIES-4
4 、ミクロシスティス・アエルギノーサ（Microcystis
aeruginosa) ＧＥＦ寄託NIES-87 、ノストック・コミュ
ーン（Nostoc commune）ＧＥＦ寄託NIES-24 およびシネ
ココッカス・エロンガタス（Synechococcus elongatus
）微工研寄託ＦＥＲMP-12393などが例示される。A) When cultivating a cyanobacterium under light irradiation using an inorganic carbon source, any cyanobacterium that releases organic acids and / or alcohols into the culture solution under dark anaerobic conditions can be used. It may be something. Preferable cyanobacteria include, for example, Spirulina platensis (Sp
irulina platensis) Deposited by NIES-39, Spirulina Foundation, Global Forum on Human Environment (hereinafter referred to as GEF)
Platensis (Spirulina platensis) GEF deposit NIES
-46, Oscillatoria limne
tica) GIES deposited NIES-36, Microcystis aeruginosa GEF deposited NIES-4
4. Microcystis aeruginosa
aeruginosa) GEF deposit NIES-87, Nostoc commune GEF deposit NIES-24 and Synechococcus elongatus
) FERMP-12393 deposited by the Japan Institute of Fabrication.
【００１０】上述した藍藻は独立栄養微生物であるた
め、光照射下で培養する段階で、ＮａＨＣＯ3 、Ｋ2 Ｈ
ＰＯ4 、ＮａＮＯ3 、Ｋ2 ＳＯ4 、ＮａＣｌ、ＭｇＳＯ
4 、ＣａＣｌ2 等の無機塩類のみを含む水溶液培地でよ
く生育し、従属栄養微生物を培養する時のように、グル
コース、ペプトン、酵母エキス等の有機添加物を培地に
加える必要はない。Since the above-mentioned cyanobacteria are autotrophic microorganisms, NaHCO 3 , K 2 H
PO 4 , NaNO 3 , K 2 SO 4 , NaCl, MgSO
4. It grows well in an aqueous medium containing only inorganic salts such as CaCl 2 , and does not require the addition of organic additives such as glucose, peptone, yeast extract, etc. to the medium as in culturing heterotrophic microorganisms.
【００１１】炭素源としては、無機炭素源のみを用い
る。無機炭素源の代表例は、二酸化炭素、重炭酸塩等で
ある。As a carbon source, only an inorganic carbon source is used. Representative examples of the inorganic carbon source include carbon dioxide, bicarbonate and the like.
【００１２】培養液としては、使用する菌株に応じて、
例えば、ＳＯＴ培地（表１）、ＭＡ培地（表２）、ＭＤ
Ｍ培地（表３）、ＢＧ−１１培地（表４）等を適宜選ん
で使用することができる。[0012] As the culture solution, depending on the strain used,
For example, SOT medium (Table 1), MA medium (Table 2), MD
M medium (Table 3), BG-11 medium (Table 4) and the like can be appropriately selected and used.
【００１３】藍藻の培養は、光照射下で行ういわゆる明
所培養である。この明所培養の照度は、たとえば、１０
０〜１００００ルックス、好ましくは５００〜３０００
ルックスである。The cultivation of blue-green algae is a so-called light culture performed under light irradiation. The illuminance of this light culture is, for example, 10
0-10000 lux, preferably 500-3000 lux
Looks.
【００１４】また、光照射下での培養段階では、通気攪
拌培養を行うことも好ましい。培養時間、培養温度、ｐ
Ｈ等の培養条件は、使用する菌株に応じて有機酸および
（または）アルコールの生産量が最大になるように適宜
設定される。一般に、培養時間は３時間以上、培養温度
は２０〜６０℃、好ましくは２５〜５５℃、培養液のｐ
Ｈは６〜１１、好ましくは７〜９が望ましい。In the culture step under light irradiation, it is also preferable to carry out aeration and stirring culture. Culture time, culture temperature, p
Culture conditions such as H are appropriately set so as to maximize the production of organic acids and / or alcohols according to the strain used. Generally, the culturing time is 3 hours or more, the culturing temperature is 20 to 60 ° C, preferably 25 to 55 ° C,
H is preferably 6 to 11, and more preferably 7 to 9.
【００１５】ｂ） つぎに、暗嫌気条件下での藍藻の培
養は、例えば下記のような操作で行われる。すなわち、
上述のように光照射下で培養した藍藻菌体を培養液から
分離し、得られた藍藻菌体を別の培養液に接種し、これ
を嫌気状態下で暗所培養する。B) Next, cultivation of cyanobacteria under dark anaerobic conditions is carried out, for example, by the following operation. That is,
The cyanobacterial cells cultured under light irradiation as described above are separated from the culture solution, and the obtained cyanobacterial cells are inoculated into another culture solution and cultured in the dark under anaerobic conditions.
【００１６】藍藻菌体を培養液から分離する手段は、濾
過、遠心分離等である。分離した藍藻菌体を接種する別
の培養液は、トリス−塩酸塩緩衝液、塩酸塩緩衝液、リ
ン酸塩緩衝液等である。これらの緩衝液のｐＨは６〜１
１、好ましくは７〜１０である。嫌気状態は、培養容器
内の空気を窒素ガス、ヘリウムガス等の不活性ガスで置
換することにより確保される。振盪培養器等を用いて攪
拌しながら培養を行うことも好ましい。培養時間、培養
温度等の培養条件は、使用する菌株に応じて有機酸およ
びアルコールの生産量が最大になるように適宜設定され
る。一般に、培養時間は５〜２０時間、培養温度は２０
〜６０℃、好ましくは２５〜５５℃であり、光を遮断し
て暗所培養を行う。Means for separating cyanobacterial cells from the culture solution include filtration and centrifugation. Another culture solution for inoculating the separated cyanobacterial cells is Tris-hydrochloride buffer, hydrochloride buffer, phosphate buffer and the like. The pH of these buffers is 6-1.
1, preferably 7 to 10. The anaerobic state is ensured by replacing the air in the culture vessel with an inert gas such as a nitrogen gas or a helium gas. It is also preferable to perform culturing while stirring using a shaking incubator or the like. The culturing conditions such as culturing time and culturing temperature are appropriately set so as to maximize the production of organic acids and alcohols according to the strain used. Generally, the culture time is 5 to 20 hours, and the culture temperature is 20 hours.
The temperature is 、 60 ° C., preferably 25-55 ° C., and the culture is performed in a dark place while blocking light.
【００１７】こうして、藍藻の培養によって培養液中
に、蟻酸、酢酸、乳酸等の低級有機酸、またはエタノー
ル等のアルコール、または有機酸とアルコールの両者が
生成される。有機酸および（または）アルコールは培養
液から採取してもよいが、そのまま培養液中の溶液の形
態でつぎの工程に使用してもよい。In this way, the culture of cyanobacteria produces a lower organic acid such as formic acid, acetic acid or lactic acid, an alcohol such as ethanol, or both an organic acid and an alcohol in the culture solution. The organic acid and / or alcohol may be collected from the culture solution, or may be used as it is in the form of a solution in the culture solution for the next step.
【００１８】有機酸およびアルコールの同定、培養液中
の濃度の測定は、例えば高速液体クロマトグラフィ、ガ
スクロマトグラフィ等を用いた手法によって行われる。
有機酸および（または）アルコールを培養液から採取
し、精製するには、一般に有機化合物の採取、精製に用
いられる手法を採用することができる。The identification of the organic acids and alcohols and the measurement of the concentration in the culture solution are performed by, for example, a technique using high performance liquid chromatography, gas chromatography or the like.
In order to collect and purify an organic acid and / or alcohol from a culture solution, a method generally used for collecting and purifying an organic compound can be employed.
【００１９】[0019]
【実施例】つぎに、本発明の実施例を幾つか挙げ、本発
明を具体的に説明する。EXAMPLES Next, the present invention will be specifically described with reference to some examples of the present invention.
【００２０】実施例１ スピルリナ・プラテンシス（Spirulina platensis ）Ｇ
ＥＦ寄託NIES-39 の湿菌体１００ｍｇをＳＯＴ培地（表
１）の培養液に接種し、温度３０℃で１２００ルックス
の光照射下１０日間培養を行った。その際、培養液に空
気を供給し、通気攪拌培養を行った。Example 1 Spirulina platensis G
100 mg of wet cells of the EF deposited NIES-39 were inoculated into the culture solution of the SOT medium (Table 1), and cultured at 30 ° C. under light irradiation of 1200 lux for 10 days. At that time, air was supplied to the culture solution, and aeration and stirring culture was performed.
【００２１】次に、培養液を濾紙を用いて濾過すること
により湿菌体約３ｇを得た。得られた湿菌体を５０ｍＬ
の三角フラスコ中のｐＨ８の２０ｍＭリン酸緩衝液３０
ｍＬに接種した。口にゴム栓を施して三角フラスコを密
閉した後、注射針を用いて窒素ガスを供給しフラスコ内
部を窒素ガスで置換することにより、嫌気状態を確保し
た。その後、振盪培養器を用いて攪拌しながら光を遮断
して１０時間、３０℃で培養を行った。その結果、蟻酸
２．４ｍＭ、酢酸９．７ｍＭ、エタノール６．２ｍＭ、
乳酸０．５ｍＭを含む培養液が得られた。濃度の測定は
ガスクロマトグラフィ法によって行った。Next, the culture broth was filtered using filter paper to obtain about 3 g of wet cells. 50 mL of the obtained wet cells
20 mM phosphate buffer pH 8 in Erlenmeyer flask
mL was inoculated. After sealing the Erlenmeyer flask with a rubber stopper on the mouth, an anaerobic state was secured by supplying nitrogen gas using an injection needle and replacing the inside of the flask with nitrogen gas. Thereafter, the cells were cultured at 30 ° C. for 10 hours while shading using a shaking incubator to cut off the light. As a result, formic acid 2.4 mM, acetic acid 9.7 mM, ethanol 6.2 mM,
A culture solution containing 0.5 mM lactic acid was obtained. The concentration was measured by a gas chromatography method.
【００２２】実施例２ スピルリナ・プラテンシス（Spirulina platensis ）Ｇ
ＥＦ寄託NIES-39 の代わりにスピルリナ・プラテンシス
（Spirulina platensis ）ＧＥＦ寄託NIES-46を用いた
こと以外は、実施例１と全く同様に操作を行った結果、
蟻酸２．２ｍＭ、酢酸１０．３ｍＭ、エタノール５．２
ｍＭ、乳酸０．３ｍＭを含む培養液が得られた。Example 2 Spirulina platensis G
The procedure was performed in exactly the same manner as in Example 1, except that Spirulina platensis GEF deposited NIES-46 was used instead of EF deposited NIES-39.
2.2 mM formic acid, 10.3 mM acetic acid, 5.2 ethanol
A culture solution containing mM and 0.3 mM lactic acid was obtained.
【００２３】実施例３ スピルリナ・プラテンシス（Spirulina platensis ）Ｇ
ＥＦ寄託NIES-39 の代わりにオシラトリア・リムネチカ
（Oscillatoria limnetica）ＧＥＦ寄託NIES-36 を用
い、ＳＯＴ培地の代わりにＭＤＭ培地（表３）を用いた
こと以外は、実施例１と全く同様に操作を行った。その
結果、蟻酸０．３ｍＭ、酢酸３．２ｍＭ、エタノール
１．０ｍＭ、乳酸１．４ｍＭを含む培養液が得られた。Example 3 Spirulina platensis G
The operation was performed in exactly the same manner as in Example 1, except that Oscillatoria limnetica GEF deposited NIES-36 was used instead of EF deposited NIES-39, and MDM medium (Table 3) was used instead of SOT medium. went. As a result, a culture solution containing 0.3 mM formic acid, 3.2 mM acetic acid, 1.0 mM ethanol, and 1.4 mM lactic acid was obtained.
【００２４】実施例４ スピルリナ・プラテンシス（Spirulina platensis ）Ｇ
ＥＦ寄託NIES-39 の代わりにシネココッカス・エロンガ
タス（Synechococcus elongatus ）微工研寄託FERM P-1
2393を用い、ＳＯＴ培地の代わりにＢＧ−１１（表４）
培地を用いたこと以外は、実施例１と全く同様に操作を
行った。その結果、蟻酸３．４ｍＭ、酢酸５．６ｍＭ、
エタノール２．８ｍＭ、乳酸４．６ｍＭを含む培養液が
得られた 。実施例５ スピルリナ・プラテンシス（Spirulina platensis ）Ｇ
ＥＦ寄託NIES-39 の代わりにノストック・コミューン
（Nostoc commune）ＧＥＦ寄託ＧＥＦ寄託NIES-24 を用
い、ＳＯＴ培地の代わりにＭＤＭ培地（表３）を用いた
こと以外は、実施例１と全く同様に操作を行った。その
結果、蟻酸０．２ｍＭ、酢酸２．５ｍＭ、乳酸１．５ｍ
Ｍを含む培養液が得られた。Example 4 Spirulina platensis G
FERM P-1 deposited by Synechococcus elongatus instead of EF deposited NIES-39
BG-11 using 2393 instead of SOT medium (Table 4)
The operation was performed in exactly the same manner as in Example 1 except that the medium was used. As a result, formic acid 3.4 mM, acetic acid 5.6 mM,
A culture solution containing 2.8 mM of ethanol and 4.6 mM of lactic acid was obtained. Example 5 Spirulina platensis G
Exactly the same as Example 1 except that the EF deposited NIES-39 was replaced with the Nostoc commune GEF deposited GEF deposited NIES-24, and the MDM medium (Table 3) was used instead of the SOT medium. The operation was performed. As a result, formic acid 0.2 mM, acetic acid 2.5 mM, lactic acid 1.5 m
A culture solution containing M was obtained.
【００２５】比較例１ 振盪培養器を用いて攪拌しながら光を遮断して培養を行
う代わりに、１２００Ｌｕｘの光照射下、振盪培養器を
用いて攪拌しながら培養を行ったこと以外は、実施例１
と全く同様に操作を行った。その結果、蟻酸１．０ｍ
Ｍ、酢酸３．７ｍＭ、エタノール３．４ｍＭ、乳酸０．
１ｍＭを含む培養液が得られた。COMPARATIVE EXAMPLE 1 Except that the culture was carried out under agitation using a shaking incubator under irradiation with light of 1200 Lux, instead of performing the culture while blocking the light while stirring using a shaking incubator. Example 1
The operation was carried out in exactly the same manner as described above. As a result, formic acid 1.0m
M, acetic acid 3.7 mM, ethanol 3.4 mM, lactic acid 0.1 mM.
A culture solution containing 1 mM was obtained.
【００２６】比較例２ 三角フラスコの口にゴム栓を施してフラスコを密閉した
後、注射針を用いて窒素ガスを供給しフラスコ内部を窒
素ガスで置換することにより、嫌気状態を確保する代わ
りに、三角フラスコの口に綿栓を施し好気条件下で培養
を行ったこと以外は、実施例１と全く同様に操作を行っ
た。その結果、蟻酸０．８ｍＭ、酢酸０．５ｍＭ、エタ
ノール０．５ｍＭを含む培養液が得られた。Comparative Example 2 A rubber stopper was placed on the mouth of an Erlenmeyer flask and the flask was sealed. Then, nitrogen gas was supplied using an injection needle and the inside of the flask was replaced with nitrogen gas. The operation was performed in exactly the same manner as in Example 1 except that the mouth of the Erlenmeyer flask was covered with a cotton plug and cultivation was performed under aerobic conditions. As a result, a culture solution containing 0.8 mM formic acid, 0.5 mM acetic acid, and 0.5 mM ethanol was obtained.
【００２７】比較例３ 三角フラスコの口にゴム栓を施してフラスコを密閉した
後、注射針を用いて窒素ガスを供給しフラスコ内部を窒
素ガスで置換することにより、嫌気状態を確保し、その
後、振盪培養器を用いて攪拌しながら光を照射して培養
を行う代わりに、三角フラスコの口に綿栓を施し好気条
件下、１２００Ｌｕｘの光照射下、振盪培養器を用いて
攪拌培養を行ったこと以外は、実施例１と全く同様に操
作を行った。その結果、蟻酸０．５ｍＭ、酢酸０．４ｍ
Ｍ、エタノール０．６ｍＭを含む培養液が得られた。Comparative Example 3 After closing the flask with a rubber stopper at the mouth of an Erlenmeyer flask, nitrogen gas was supplied using an injection needle and the inside of the flask was replaced with nitrogen gas, thereby ensuring an anaerobic state. Instead of irradiating light with stirring while using a shaking incubator, instead of culturing by irradiating light with a cotton plug in the mouth of an Erlenmeyer flask, stirring culture using a shaking incubator under 1200 Lux light irradiation under aerobic conditions. Except for what was done, the operation was performed exactly as in Example 1. As a result, formic acid 0.5 mM, acetic acid 0.4 m
A culture solution containing M and 0.6 mM of ethanol was obtained.
【００２８】[0028]
【表１】 [Table 1]
【表２】 [Table 2]
【表３】 [Table 3]
【表４】 [Table 4]
【表５】 [Table 5]
【００２９】[0029]
【発明の効果】本発明により、光合成を行うため無機物
のみを炭素源として、有機炭素源もしくはＨ2 等の還元
物質を必要とすることなく、藍藻の培養により無機炭素
源から有機酸および（または）アルコールを高収率で取
得することができる。Industrial Applicability According to the present invention, organic acids and (or or) can be obtained from an inorganic carbon source by culturing cyanobacteria, using only inorganic substances as a carbon source for photosynthesis and without requiring an organic carbon source or a reducing substance such as H 2. ) The alcohol can be obtained in high yield.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:89） (Ｃ１２Ｐ 7/40 Ｃ１２Ｒ 1:89） (Ｃ１２Ｐ 7/54 Ｃ１２Ｒ 1:89） (Ｃ１２Ｐ 7/56 Ｃ１２Ｒ 1:89） (72)発明者 浅田 泰男 茨城県つくば市春日１丁目102番506号 (72)発明者 常盤 豊 茨城県土浦市桜ケ丘46−12 審査官 村上 騎見高 (56)参考文献 ＢＩＯＴＥＣＨＮＯＬ．ＢＩＯＥＮ Ｇ．，ＶＯＬ．28，ＮＯ．７（1986）, Ｐ．1014−1023──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 6 Identification number Agency reference number FI Technical display location C12R 1:89) (C12P 7/40 C12R 1:89) (C12P 7/54 C12R 1:89) (C12P 7/56 C12R 1:89) (72) Inventor Yasuo Asada 1-102-506, Kasuga, Tsukuba-shi, Ibaraki Prefecture (72) Inventor Yutaka Tokiwa 46-12 Sakuragaoka, Tsuchiura-shi, Ibaraki Pref. 56) References BIOTECHNOL. BIOEN G. , VOL. 28, NO. 7 (1986), p. 1014−1023
Claims (1)
素源を用いて培養し、その培養物をさらに暗嫌気条件下
で培養することにより、有機酸および（または）アルコ
ールを取得する、藍藻を用いる有機分泌物の製造方法。An organic acid and / or alcohol is obtained by culturing a cyanobacterium under light irradiation using an inorganic carbon source as a carbon source, and further culturing the culture under dark anaerobic conditions. A method for producing organic secretions using cyanobacteria.
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|---|---|---|---|
| JP4268960A JP2729883B2 (en) | 1992-10-07 | 1992-10-07 | Method for producing organic secretions using cyanobacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4268960A JP2729883B2 (en) | 1992-10-07 | 1992-10-07 | Method for producing organic secretions using cyanobacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH08168389A JPH08168389A (en) | 1996-07-02 |
| JP2729883B2 true JP2729883B2 (en) | 1998-03-18 |
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ID=17465693
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|---|---|---|---|
| JP4268960A Expired - Lifetime JP2729883B2 (en) | 1992-10-07 | 1992-10-07 | Method for producing organic secretions using cyanobacteria |
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|---|---|---|---|---|
| JP2001354407A (en) * | 2000-06-08 | 2001-12-25 | Rikogaku Shinkokai | Method for removing and recovering carbon dioxide by indigo-blue bacteria |
| JP4669990B2 (en) * | 2005-02-24 | 2011-04-13 | 学校法人東京農業大学 | Lactic acid production method |
| BRPI0821508B1 (en) | 2007-12-11 | 2018-12-26 | Synthetic Genomics Inc | Cell culture comprising a recombinant cyanobacterium of synechococcus elongatus species and method for converting inorganic carbon to fatty acids. |
| WO2018051916A1 (en) * | 2016-09-14 | 2018-03-22 | 国立大学法人神戸大学 | Method for producing organic acid |
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1992
- 1992-10-07 JP JP4268960A patent/JP2729883B2/en not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| ＢＩＯＴＥＣＨＮＯＬ．ＢＩＯＥＮＧ．，ＶＯＬ．２８，ＮＯ．７（１９８６），Ｐ．１０１４−１０２３ |
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