JP2674674B2 - Removal method of nitrate nitrogen in roughage - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of use] The present invention is contained in roughage, and when ingested by livestock and humans, it combines with other substances in the body to exhibit poisoning symptoms,
The present invention also relates to a method for removing nitrate nitrogen in a roughage which is adapted to remove nitrate nitrogen that produces a carcinogen.

[Conventional technology]

Conventionally, it has been known that roughage such as grass contains nitric nitrogen such as nitric acid and nitrate. It is also known that when these are ingested by livestock and humans, they become nitrite in the body and combine with other substances to cause nitrate poisoning symptoms, or to generate carcinogenic nitrosamine substances. .

[Problems to be solved by the invention]

However, as mentioned above, when ingested by livestock and humans, it becomes nitrous acid in the body, causing poisoning symptoms by combining with other substances, and nitrate nitrogen which is a source of producing carcinogenic substances, The current situation is that no technology has been developed to actively remove it from roughage.

The present invention has been made in view of the above circumstances, and an object thereof is to provide a method for easily removing nitrate nitrogen, which is a toxic substance, after ingesting a roughage and entering the body.

[Means for solving the problem]

In order to achieve the above object, the present invention, in a roughage containing nitrate nitrogen, Pichia (Pichia anomala) genus species killer strain, which is a strain having assimilating nitrate reductase in yeast, Lactobacillus plantarum (Lactobacillus plantarum) KTC4-3 strain was inoculated together with this, and after using this enzyme to reduce nitrate nitrogen,
Further, it is characterized in that nitrate nitrogen is removed from the roughage by converting it into a bacterial cell component and growing it.

[Action]

By the above-mentioned method, nitrogen nitrate is reduced by yeast and lactic acid bacteria, further converted into bacterial cell components and removed, and neither poisoning symptoms nor carcinogenic substances are produced.

〔Example〕

Hereinafter, with reference to the drawings and the attached table, examples of using the present invention as a raw material for sweet sorghum will be described.

(1) Implementation method [Strains used] Yeast is a storage and collection strain and a total of more than 20 isolates, and is a Pichia species that is resident in silage. A strong killer strain was used. Lactobacillus is Lactobacillus plantarum.
s plantarum) KTC4-3 strain was used. The yeast was shake-cultured in yeast extract 0.1% Czapec Dox medium for 3 days with shaking, and the lactic acid bacteria were also cultured in MRS medium in 2%.
After culturing for one day, the cells were inoculated with the inoculum size shown in Table 1.

[Materials] In Experiments 1 and 2 (preliminary test), sweet sorghum cultivated in the field was used as a material, and after finely cutting, a residue of only the stem was prepared with a three-roll type squeezing machine. 10k fresh food
g, also in Experiment 3 (this test), 1
40 kg / district tested. At that time, in Experiment 1, potassium nitrate was added to NO ₃ to supplement 0.4% / fresh matter, and in Experiments 2 and 3, 0.2% was supplemented.

[Treatment Section] The three treatments were carried out by the four treatment sections shown in Table 2.

[Pretreatment step] The residue after inoculation with yeast and lactic acid bacteria was deposited in the room for 2 days at a thickness of 3.5 cm in the preliminary test and 15 cm in the same test. During this time, after repeating 3 to 5 times (see FIG. 1), the material was packed in silos.

[Analysis / Measurement method] Freeze-drying method is used for water, ion chromatography method and Bremner method are used for nitrate state, etc. Diluted plates were used.

(2) Implementation results. In the preliminary test, 2 days after the inoculation, the yeast inoculation area (3, 4 areas)
The product temperature was about 3 ° C higher than that in the non-inoculation area (1, 2 areas). In addition, in this test, an increase in the product temperature was observed about 16 hours after the inoculation. In particular, fever was remarkable in the 4 groups where yeast and lactic acid bacteria were used together (see FIG. 1).

.3 No significant increase in the number of lactic acid bacteria was observed in the lactic acid bacteria inoculated area (2, 4 areas). On the other hand, yeast showed remarkable growth, reaching 8.88 to 9.67 cfu / g (common logarithmic display, colony forming unit) in the 3rd and 4th plots (see Table 2).

. In all of the experiments, the loss of nitrate nitrogen (NO ₃ ) was more remarkable in the yeast inoculation group than in the yeast non-inoculation group. Especially, 66.5 to 76.2% in the preliminary test, about 7 It showed twice as high disappearance (see Table 2).

.Nitrite (N
O ₂ ) was not detected (see FIG. 2), and ammonia nitrogen was also not detected to a very small amount. In addition, since the yeast has seen a remarkable increase in cfu during this period, it can be said that NO ₃ that disappeared in the yeast inoculation zone was converted and removed as it was into the bacterial cell component.

Although the silo is unopened, the silage prepared by filling it into a PVC silo (preliminary test) did not detect the occurrence of defective fermentation in any silo in appearance.

From this test result, the method for removing nitrate nitrogen of the present invention shows that long crops for grass and feed (rough forage) and silage prepared by fermenting them and similarly containing nitrate nitrogen. It is also applicable to garden vegetables such as carrots and radish that are processed into pickles.

〔The invention's effect〕

As described above, according to the method for removing nitrate nitrogen in the roughage of the present invention, the roughage containing nitrate nitrogen, by inoculating the yeast and lactic acid bacteria having nitrate assimilation ability, the nitrate nitrogen is a fungal component. Since it can be converted into, erased, and removed, the work is simple, and it is possible to prevent the danger of entering into the body and causing poisoning symptoms or producing carcinogens.

[Brief description of the drawings]

FIG. 1 shows an embodiment of the present invention, and is a graph showing changes in material temperature after inoculation with yeast and lactic acid bacteria, and FIG. 2 is an ion chromatogram of a water extract of sweet sorghum juice residue.

Claims (1)

(57) [Claims] 1. A killer strain of Lactobacillus plantarum which is a lactic acid bacterium is used as a roughage containing nitrate nitrogen, in which a killer strain of the genus Pichia anomala, which is a strain having assimilating nitrate reductase in yeast, is used. (Lactobacillus
plantarum) KTC4-3 strain was inoculated together, nitrate nitrogen was reduced using this enzyme, and then converted to bacterial components and grown to remove nitrate nitrogen from the roughage. Method for Removing Nitrate-Nitrogen from Roughage.