JP2643003B2 - Cell culture bag - Google Patents
Cell culture bagInfo
- Publication number
- JP2643003B2 JP2643003B2 JP7618190A JP7618190A JP2643003B2 JP 2643003 B2 JP2643003 B2 JP 2643003B2 JP 7618190 A JP7618190 A JP 7618190A JP 7618190 A JP7618190 A JP 7618190A JP 2643003 B2 JP2643003 B2 JP 2643003B2
- Authority
- JP
- Japan
- Prior art keywords
- bag
- cell culture
- density polyethylene
- weight
- low
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Description
本発明は細胞培養用バツグに関し，更に詳しくは採取
した細胞に培地を加えて細胞を培養するための酸素透過
性，透明の優れた細胞培養用バツグに関する。The present invention relates to a bag for cell culture, and more particularly to a bag for cell culture having excellent oxygen permeability and transparency for culturing cells by adding a medium to collected cells.
従来，哺乳動物の付着細胞や懸濁細胞を生体外で行う
培養は，ガラス製容器の中で培地と共に行われてきた
が，空気中の酸素の流通が悪いために古くなった培地を
新しい培地と頻繁に交換しながら培養を続ける必要があ
り，培養環境もその都度pHが変わったりして，一定にな
らない欠点があった。 かかるガラス製培養容器の欠点を改良した容器とし
て，イオノマー樹脂からなるプラスチツク容器を細胞培
養に使用する方法が特60−160881号公報に開示されてい
る。Conventionally, culture of mammalian adherent cells or suspension cells in vitro has been performed together with the culture medium in a glass container, but the old culture medium was replaced with a new culture medium due to poor oxygen flow in the air. It was necessary to continue cultivation while frequently exchanging with, and the pH of the cultivation environment also changed each time, and there was a drawback that it was not constant. As a container in which the drawbacks of the glass culture container are improved, a method of using a plastic container made of an ionomer resin for cell culture is disclosed in Japanese Patent Publication No. 60-16081.
しかしながら，この容器は強度が十分でないため破れ
やすく，取扱いが難しいという欠点があった。更にこの
容器はエチレンとアクリル酸などの不飽和カルボン酸の
共重合体のカルボキシル基をNa,K,Cu,Zn,Feなどのカチ
オン金属で架橋した樹脂からなるために，重金属や蒸発
残留物が多く，樹脂中のカチオン金属によっては細胞に
対して毒性を有するものもあった。However, this container has a drawback that it is easily broken due to insufficient strength and is difficult to handle. Furthermore, since this container is made of a resin in which the carboxyl group of a copolymer of ethylene and an unsaturated carboxylic acid such as acrylic acid is cross-linked with a cationic metal such as Na, K, Cu, Zn or Fe, heavy metals and evaporation residues are removed. In many cases, some cationic metals in the resin were toxic to cells.
【課題を解決するための手段及び作用】 本発明は，（ａ）エチレンと炭素数６〜８のαオレフ
インの共重合体からなる線状低密度ポリエチレン70〜95
重量％（ｂ）低密度ポリエチレン30〜５重量％の組成の
フイルムからなる細胞培養用バツグを要旨とする。 本発明において，線状低密度ポリエチレンと低密度ポ
リエチレンの組み合わせのフイルムを用いるが，これは
フイルムの強度・透明性・酸素透過性を考慮して一番好
ましいポリマーアロイとなるからである。これらの分子
量はフイルム形成能があればよく，両者とも10,000〜25
0,000が好ましい。 本発明に用いる線状低密度ポリエチレンは，エチレン
と炭素数６〜８のαオレフインの共重合体で市販のもの
を用いればよいが，この場合αオレフインの炭素数を６
以上に限定する必要がある。 この炭素数が５以下であると，強度が不十分になると
いう問題がある。なおこの炭素数が９以上でも特に問題
はないが，これの製造が難しいという問題はある。 本発明に用いるフイルムのポリマーアロイにおいて，
線状低密度ポリエチレンが70〜95重量％，すなわち，低
密度ポリエチレン30〜５重量％の組成に限定する必要が
ある。 これは線状低密度ポリエチレンが70重量％以上含まれ
ないと透明性・耐熱性・強度の点で好ましくなく，これ
が95重量％を超えると，加工性・ヒートシール性・透明
性・柔軟性の点で好ましくないことになる。 バツグの成形は前記２種のポリマーをブレンダー又は
ミキサーに投入して均一に混合した後，重合体混合物を
溶解し，細胞培養用バツグの形をした金型内に溶解押し
出し・ブロー成形するか，又はフイルム状に押し出した
後，端部をヒートシールして細胞培養用バツグを製造す
る。なお，バツグを形成するフイルムの厚さは細胞の種
類に応じて適宜選択でき，例えば50〜300μである。SUMMARY OF THE INVENTION The present invention relates to (a) a linear low-density polyethylene 70-95 comprising a copolymer of ethylene and α-olefin having 6 to 8 carbon atoms.
A gist of the present invention is a cell culture bag comprising a film having a composition of 30% to 5% by weight of low-density polyethylene (b). In the present invention, a film composed of a combination of linear low-density polyethylene and low-density polyethylene is used because it is the most preferable polymer alloy in consideration of the strength, transparency and oxygen permeability of the film. These molecular weights need only have a film forming ability, and both have a molecular weight of 10,000 to 25.
0,000 is preferred. The linear low-density polyethylene used in the present invention may be a commercially available copolymer of ethylene and α-olefin having 6 to 8 carbon atoms. In this case, the α-olefin has 6 carbon atoms.
It is necessary to limit to the above. When the carbon number is 5 or less, there is a problem that the strength becomes insufficient. There is no particular problem if the number of carbon atoms is 9 or more, but there is a problem that its production is difficult. In the polymer alloy of the film used in the present invention,
It is necessary to limit the composition of the linear low-density polyethylene to 70 to 95% by weight, that is, 30 to 5% by weight of the low-density polyethylene. This is not desirable in terms of transparency, heat resistance and strength unless linear low-density polyethylene is contained in an amount of 70% by weight or more. This is undesirable in that respect. To form a bag, the two types of polymers are charged into a blender or a mixer and uniformly mixed, and then the polymer mixture is dissolved and extruded and blow-molded into a mold in the shape of a bag for cell culture. Alternatively, after extruding in a film shape, the ends are heat-sealed to produce a cell culture bag. The thickness of the film forming the bag can be appropriately selected according to the type of the cell, and is, for example, 50 to 300 μm.
エチレンと炭素数６のαオレフインの共重合体からな
る線状低密度ポリエチレン（住友化学工業株式会社製FZ
−102）80重量％と低密度ポリエチレン（住友化学工業
株式会社製Ｆ−102）20重量％をミキサーで混合し,200
℃で溶解してフイルム状に押し出し，厚さ100μのフイ
ルムを製造した。このフイルム２枚の端部を熱融着し
て,300ml容量の細胞培養用バツグを形成した。 このバツグについて,2.5Mradのγ線で滅菌後，酸素透
過率・炭酸ガス透過率・水蒸気透過率・溶出物試験を下
記に示す方法で試験した。その結果を第１表に示す。 ［酸素透過率・炭酸ガス透過率］ ASTM−Ｄ−1434に従って測定。 ［水蒸気透過率］ JIS−Ｚ−0208に従って測定。 ［溶出物試験］ 医療用プラスチツク自主規格（日本医療用プラスチツ
ク協会作成）を準用する。 第１表から明らかなように，本発明バツグは酸素透過
率および炭酸ガス透過率がよく，水蒸気透過率が低い。
また溶出物試験結果はいずれも医療用プラスチツクの自
主規格に合格しており，細胞培養用バツグとして優れた
特性を有している。 （比較例１） エチレンと炭素数４のαオレフインの共重合体からな
る線状低密度ポリエチレン（住友化学工業株式会社製FA
−201）80重量％と低密度ポリエチレン（住友化学工業
株式会社製Ｆ−208）20重量％の混合物を用いて実施例
１と同様のバツグを作り，実施例１と強度及び透明性を
比較した。その結果を第２表に示す。 （比較例２） エチレンと炭素数６のαオレフインの共重合体からな
る線状低密度ポリエチレン（住友化学工業株式会社製FA
−201）60重量％と低密度ポリエチレン（住友化学工業
株式会社製Ｆ−208）40重量％の混合物を用いて実施例
１と同様のバツグを作り，実施例１と強度及び透明性を
比較した。その結果を第２表に示す。 （比較例３） エチレンと炭素数６のαオレフインの共重合体からな
る線状低密度ポリエチレン（住友化学工業株式会社製FA
−201）97重量％と低密度ポリエチレン（住友化学工業
株式会社製Ｆ−208）３重量％の混合物を用いて実施例
１と同様のバツグを作り，実施例１と強度及び透明性を
比較した。その結果を第２表に示す。 なお上記比較試験において，強度は23℃において引張
強度で評価し,JIS−Ｚ−1702に従って測定した。そして
透明性は紫外分光光度計を用いて,450nmにおける光透過
率を測定した。 Linear low-density polyethylene made of a copolymer of ethylene and α-olefin having 6 carbon atoms (FZ manufactured by Sumitomo Chemical Co., Ltd.)
-102) 80% by weight and 20% by weight of low-density polyethylene (F-102 manufactured by Sumitomo Chemical Co., Ltd.) are mixed with a mixer,
The mixture was melted at ℃ and extruded into a film to produce a 100 μm thick film. The ends of the two films were heat-sealed to form a 300-ml cell culture bag. The bag was sterilized with 2.5 Mrad of γ-ray, and then tested for oxygen permeability, carbon dioxide gas permeability, water vapor permeability, and eluate by the following methods. Table 1 shows the results. [Oxygen permeability / carbon dioxide gas permeability] Measured according to ASTM-D-1434. [Water vapor permeability] Measured according to JIS-Z-0208. [Eluent test] The voluntary standard for medical plastics (prepared by Japan Medical Plastics Association) shall be applied mutatis mutandis. As is clear from Table 1, the bag of the present invention has good oxygen permeability and carbon dioxide gas permeability, and low water vapor permeability.
In addition, all the eluate test results have passed the voluntary standard for medical plastics, and have excellent characteristics as a bag for cell culture. (Comparative Example 1) Linear low-density polyethylene made of a copolymer of ethylene and α-olefin having 4 carbon atoms (FA manufactured by Sumitomo Chemical Co., Ltd.)
-201) A bag similar to that of Example 1 was prepared using a mixture of 80% by weight and 20% by weight of low-density polyethylene (F-208 manufactured by Sumitomo Chemical Co., Ltd.), and the strength and transparency were compared with those of Example 1. . Table 2 shows the results. (Comparative Example 2) Linear low-density polyethylene made of a copolymer of ethylene and α-olefin having 6 carbon atoms (FA manufactured by Sumitomo Chemical Co., Ltd.)
-201) A bag similar to that of Example 1 was prepared using a mixture of 60% by weight and 40% by weight of low-density polyethylene (F-208 manufactured by Sumitomo Chemical Co., Ltd.), and strength and transparency were compared with Example 1. . Table 2 shows the results. (Comparative Example 3) Linear low-density polyethylene made of a copolymer of ethylene and α-olefin having 6 carbon atoms (FA manufactured by Sumitomo Chemical Co., Ltd.)
-201) A bag similar to that of Example 1 was prepared using a mixture of 97% by weight and 3% by weight of low-density polyethylene (F-208 manufactured by Sumitomo Chemical Co., Ltd.), and the strength and transparency were compared with those of Example 1. . Table 2 shows the results. In the above comparative test, the strength was evaluated by tensile strength at 23 ° C. and measured according to JIS-Z-1702. And the transparency was measured light transmittance at 450nm using ultraviolet spectrophotometer.
【発明の効果】 本発明のポリマーアロイからなる細胞培養用バツグは
酸素透過性・炭酸ガス透過性に優れ，水蒸気不透過性の
性質を有するので，フイルムを通しての酸素・炭酸ガス
が自由に透過し，細胞培養用バツグとして最適である。 更にこのバツグは重金属などの溶出物が少なくバツグ
材料から滲出する不純物が細胞の培養を妨げる心配もな
く，安全に使用可能である。 更にこのバツグは透明性・引張強度にも優れ，細胞培
養用バツグとして十分にその特性を有するものである。The bag for cell culture comprising the polymer alloy of the present invention has excellent oxygen permeability and carbon dioxide gas permeability, and has water vapor impermeability, so that oxygen and carbon dioxide gas can be freely permeated through the film. It is most suitable as a bag for cell culture. Further, the bag has a small amount of eluted substances such as heavy metals and can be safely used without any concern that impurities oozing out of the bag material hinder cell culture. Further, this bag is excellent in transparency and tensile strength, and has sufficient properties as a bag for cell culture.
Claims (1)
インの共重合体からなる線状低密度ポリエチレン70〜95
重量％（ｂ）低密度ポリエチレン30〜５重量％の組成の
フイルムからなる細胞培養用バツグ。1. A linear low-density polyethylene 70 to 95 comprising (a) a copolymer of ethylene and α-olefin having 6 to 8 carbon atoms.
(B) A low-density polyethylene bag for cell culture comprising a film having a composition of 30 to 5% by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7618190A JP2643003B2 (en) | 1990-03-26 | 1990-03-26 | Cell culture bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7618190A JP2643003B2 (en) | 1990-03-26 | 1990-03-26 | Cell culture bag |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03277268A JPH03277268A (en) | 1991-12-09 |
| JP2643003B2 true JP2643003B2 (en) | 1997-08-20 |
Family
ID=13597945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7618190A Expired - Fee Related JP2643003B2 (en) | 1990-03-26 | 1990-03-26 | Cell culture bag |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2643003B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012032761A1 (en) | 2010-09-06 | 2012-03-15 | 東洋製罐株式会社 | Multilayer film and cell culture container |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0698756A (en) * | 1992-09-18 | 1994-04-12 | Nissho Corp | Bag for culturing adhesive cell |
| JP2007104975A (en) | 2005-10-14 | 2007-04-26 | Toyo Seikan Kaisha Ltd | Culture container and culture method |
| JP7155513B2 (en) * | 2017-12-14 | 2022-10-19 | 東洋製罐グループホールディングス株式会社 | Culture vessel substrate and culture vessel |
-
1990
- 1990-03-26 JP JP7618190A patent/JP2643003B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012032761A1 (en) | 2010-09-06 | 2012-03-15 | 東洋製罐株式会社 | Multilayer film and cell culture container |
| KR101484865B1 (en) * | 2010-09-06 | 2015-01-20 | 도요세이칸 그룹 홀딩스 가부시키가이샤 | Multilayer film and cell culture container |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03277268A (en) | 1991-12-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |