JP2641161B2 - Yeast extract and its production method - Google Patents

Yeast extract and its production method

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Publication number
JP2641161B2
JP2641161B2 JP5215061A JP21506193A JP2641161B2 JP 2641161 B2 JP2641161 B2 JP 2641161B2 JP 5215061 A JP5215061 A JP 5215061A JP 21506193 A JP21506193 A JP 21506193A JP 2641161 B2 JP2641161 B2 JP 2641161B2
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Japan
Prior art keywords
yeast extract
concentration
filtration
stock solution
production method
Prior art date
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Expired - Fee Related
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JP5215061A
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Japanese (ja)
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JPH0751024A (en
Inventor
誉昌 渡辺
敬子 佐藤
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Asahi Breweries Ltd
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Asahi Breweries Ltd
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Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【0001】[0001]

【産業上の利用分野】本発明は、天然調味料として広く
利用されている酵母エキスとその製造法に関する。更に
詳しくは、高濃度の酵母エキスを冷蔵ないし室温下で保
存しても、清澄で流動性のよい酵母エキスの製造法に関
する。
The present invention relates to a yeast extract widely used as a natural seasoning and a method for producing the same. More specifically, the present invention relates to a method for producing a clear and fluid yeast extract even when a high-concentration yeast extract is stored under refrigeration or at room temperature.

【0002】[0002]

【従来の技術】酵母エキスは、天然調味料として、肉エ
キス、野菜エキス、魚介類エキスなどと共に化学調味料
にはない複雑な呈味性を有しているため、近年広く利用
されている。酵母エキスの製造法としては、酵母エキス
原液を酵素処理(特開昭54−17167、特開昭58
−86060、特開昭62−289161、特開昭63
−112965)、加熱加圧処理(特開昭56−156
72、特開昭61−181356)、超臨界処理(特開
平1−10960)、限外濾過膜による処理(特開平1
−112966、特開平2−219560)などを特徴
としたものが開示されているが、これらの製造法は、酵
母エキスの酵母臭の除去や呈味の改善、収率をアップさ
せるなどを目的としている。また、高濃度の酵母エキス
の製造法としては、限外濾過膜による濃縮(特開平2−
219560)、超高圧の利用(特開平2−25505
9)などがあげられる。さらに酵素処理することによっ
て、透明な酵母エキスを得る製造法(特開平04−14
4667)が開示されている。
2. Description of the Related Art Yeast extract has been widely used in recent years as a natural seasoning because it has a complex taste that is not found in chemical seasonings together with meat extracts, vegetable extracts, seafood extracts and the like. As a method for producing yeast extract, an undiluted solution of yeast extract is treated with an enzyme (JP-A-54-17167, JP-A-58-1983).
-86060, JP-A-62-289161, JP-A-63
-112965), heating and pressurizing treatment (JP-A-56-156)
72, JP-A-61-181356), supercritical treatment (JP-A-1-10960), treatment with an ultrafiltration membrane (JP-A-1-181960)
JP-A-112966, Japanese Patent Application Laid-Open No. 2-219560) are disclosed, but these production methods are intended to remove yeast odor of yeast extract, improve taste, increase yield, and the like. I have. As a method for producing a high-concentration yeast extract, concentration using an ultrafiltration membrane (Japanese Unexamined Patent Application Publication No.
219560), use of ultra-high pressure (JP-A-2-25505)
9) and the like. A process for obtaining a transparent yeast extract by further enzymatic treatment (Japanese Patent Laid-Open No. 04-14)
4667).

【0003】[0003]

【発明が解決しようとする課題】一般に高濃度酵母エキ
ス(総固形分濃度、30〜75w/w%)は、酵母エキ
ス原液中の不溶性成分の粒度が小さく、ケーク比抵抗が
大きい難濾過性の溶液であることから、従来、高濃度の
酵母エキスを得る製造法として、低濃度(総固形分濃
度、1〜30w/w%)で濾過後、濃縮を行っていた。
しかしながら、この製造法では、低濃度で濾過した直接
の酵母エキスは清澄であるが、濃縮し高濃度になるに従
い、濁りが生じる。その結果、麺つゆ、ラーメンスープ
あるいはタレなどの清澄性が求められる食品へ添加した
場合、濁りが生じたり、添加後の保存温度により、濁り
の量が変化するなどの問題がある。また、先行技術に記
載の透明な酵母エキスを得る製造法では、酵素を使用す
るため工程が煩雑となり、更に設備や酵素などへの費用
がかかり経済的な負担が大きい。一方、輸送の際に、コ
ンテナ包装などの包装形態を使用した場合、従来の高濃
度の酵母エキスは、流動性が悪いため、溶液の排出が困
難であり、さらにそのため取扱いに時間がかかるなど、
酵母エキスの調味料としての利用拡大の障害となってい
る。このように従来の製造法で高濃度の酵母エキスを得
ると、濁りが生じたり、流動性が悪いため、調味料とし
て利用しにくいという問題点がある。そこで本発明の課
題は、高濃度の酵母エキスを冷蔵ないし室温下で保存し
ても、清澄で流動性がよい酵母エキスとその製造法を提
供することにある。
Generally, high-concentration yeast extract (total solid content, 30-75 w / w%) has a small particle size of the insoluble component in the yeast extract stock solution and a large cake specific resistance. Since the solution is a solution, conventionally, as a production method for obtaining a high-concentration yeast extract, concentration was performed after filtration at a low concentration (total solid content concentration, 1 to 30 w / w%).
However, in this production method, the yeast extract directly filtered at a low concentration is clear, but becomes turbid as the concentration is increased to a high concentration. As a result, when added to foods that require clarity, such as noodle soup, ramen soup or sauce, there are problems such as turbidity and the amount of turbidity changing depending on the storage temperature after addition. Further, in the production method for obtaining a transparent yeast extract described in the prior art, the steps are complicated because enzymes are used, and the cost for equipment and enzymes is high, and the economic burden is large. On the other hand, when using a packaging form such as container packaging during transportation, the conventional high-concentration yeast extract has poor fluidity, so it is difficult to discharge the solution, and furthermore, it takes time to handle.
This is an obstacle to expanding the use of yeast extract as a seasoning. As described above, when a yeast extract having a high concentration is obtained by the conventional production method, there is a problem that it is difficult to use as a seasoning due to turbidity and poor fluidity. Therefore, an object of the present invention is to provide a yeast extract that is clear and has good fluidity even when a high-concentration yeast extract is stored under refrigeration or at room temperature, and a method for producing the yeast extract.

【0004】[0004]

【課題を解決するための手段】本発明者らは、前記課題
を解決するために鋭意検討を行った結果、酵母エキス原
液を−18〜5℃で凍らないように7日〜30日間、冷
却後、濾過を行う製造法によって冷蔵、室温下で保存し
ても、清澄で流動性のある高濃度の酵母エキスを得て、
本発明を完成した。更に、冷却する前処理として、加熱
処理工程を加えることにより、効率良く酵母エキスが得
られることを見い出した。すなわち、本発明の第一は、
酵母エキスの総固形分濃度が30〜75w/w%、冷蔵
ないし室温下で清澄で、粘度が10〜1000cpsと
流動性がよいことを特徴とする酵母エキスに関する。本
発明の第二は、酵母エキス原液を、−18〜5℃の凍ら
ない条件下に7日〜30日間保持した後、濾過を行うこ
とを特徴とする前記酵母エキスの製造法に関する。前記
製造法においては、冷却前に酵母エキス原液をpH4.
5〜7.0に調製し、80〜135℃で1〜60分加熱
処理を行うことが好ましい。前記製造法においては、酵
母エキスの温度が、−18〜5℃の凍らない条件下に保
ったまま濾過を行うことが好ましい。また、前記製造法
においては、酵母エキス原液中の酵母エキス濃度を、2
0〜55w/w%とすることが好ましい。前記製造法に
おいては、酵母がビール酵母であることができる。
Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems. As a result, the yeast extract stock solution was cooled for 7 to 30 days so as not to freeze at −18 to 5 ° C. After that, even if refrigerated by the production method of performing filtration, even if stored at room temperature, to obtain a clear and fluid high concentration yeast extract,
The present invention has been completed. Furthermore, it has been found that a yeast extract can be efficiently obtained by adding a heat treatment step as a pretreatment for cooling. That is, the first of the present invention is:
The present invention relates to a yeast extract characterized by a total solid content of the yeast extract of 30 to 75 w / w%, a clarity under refrigeration or room temperature, and a viscosity of 10 to 1000 cps and good fluidity. The second aspect of the present invention relates to a method for producing the yeast extract, comprising maintaining the undiluted yeast extract at -18 to 5 ° C under non-freezing conditions for 7 to 30 days, followed by filtration. In the above production method, the yeast extract stock solution is cooled to pH 4 before cooling.
It is preferable that the temperature is adjusted to 5 to 7.0 and the heat treatment is performed at 80 to 135 ° C. for 1 to 60 minutes. In the above-mentioned production method, it is preferable to perform the filtration while maintaining the temperature of the yeast extract at -18 to 5 ° C so as not to freeze. In the above-mentioned production method, the yeast extract concentration in the yeast extract stock solution is adjusted to 2
It is preferable to be 0 to 55 w / w%. In the above production method, the yeast can be brewer's yeast.

【0005】以下、本発明について詳しく説明する。酵
母エキス濃度が20〜55w/w%、その他の固形分濃
度10〜20w/w%であって、総固形分濃度が30〜
75%である酵母エキス原液をpH4.5〜7.0、好
ましくは、pH4.5〜5.0に調製し、温度80〜1
35℃、好ましくは110〜135℃で、1〜60分、
好ましくは1〜5分で加熱処理する。加熱処理後、−1
8〜5℃の凍らない条件下に7日〜30日間、好ましく
は7日〜9日間、保持し、透過率0.5〜2.0(da
rcy)、好ましくは0.8〜1.0(darcy)の
ケイソウ土などの濾過助剤を酵母エキス原液に対して3
〜10w/w%、好ましくは3〜6w/w%添加して、
濾過を行い、その濾過液としての酵母エキスを得た。こ
の酵母エキスは、その総固形分濃度が30〜75w/w
%、好ましくは、30〜60w/w%と高濃度であり、
冷蔵ないし室温保存下でも清澄で、粘度が10〜100
0cps、好ましくは10〜500cpsと流動性がよ
いことを特徴とするものである。本発明の酵母エキスの
製造法は、酵母エキス原液を冷却保持後、濾過すること
により目的とする酵母エキスを得ることができるが、冷
却する前に加熱処理する工程を追加することで、濾過の
際の濾過時間を短縮することができ、必要濾過面積も少
なくてすむので、更に経済性がよく、効率よく酵母エキ
スを得ることができる。なお、酵母エキス原液の製造法
として、自己消化法、加圧処理法、酵素分解法などがあ
げられるが、酵母エキス濃度が20〜55w/w%、そ
の他の固形分濃度10〜20w/w%、総固形分濃度が
30〜75w/w%である酵母エキス原液が得られる製
造法であれば、特に限定されるものではない。また、濾
過法としては、前述のケイソウ土濾過のほか、メンブラ
ン濾過、限外濾過、精密濾過などを用いることができ
る。
Hereinafter, the present invention will be described in detail. The yeast extract concentration is 20 to 55 w / w%, the other solid concentration is 10 to 20 w / w%, and the total solid concentration is 30 to
A 75% yeast extract stock solution is adjusted to pH 4.5-7.0, preferably pH 4.5-5.0, and the temperature is adjusted to 80-1.
35 ° C., preferably at 110 to 135 ° C., for 1 to 60 minutes,
The heat treatment is preferably performed for 1 to 5 minutes. After heat treatment, -1
It is kept for 7 to 30 days, preferably for 7 to 9 days under the condition of non-freezing at 8 to 5 ° C, and has a transmittance of 0.5 to 2.0 (da).
rcy), preferably 0.8 to 1.0 (darcy) of a filter aid such as diatomaceous earth with respect to 3% of the yeast extract stock solution.
-10 w / w%, preferably 3-6 w / w%,
Filtration was performed to obtain a yeast extract as the filtrate. This yeast extract has a total solid content concentration of 30 to 75 w / w.
%, Preferably 30 to 60% w / w,
It is clear even under refrigeration or room temperature storage and has a viscosity of 10 to 100.
The fluidity is good at 0 cps, preferably 10 to 500 cps. The method for producing the yeast extract of the present invention can obtain the target yeast extract by filtering after keeping the yeast extract stock solution cooled, but by adding a heat treatment step before cooling, the filtration is performed. In this case, the filtration time can be shortened and the required filtration area can be reduced, so that the yeast extract can be obtained more economically and efficiently. In addition, examples of a method for producing a yeast extract stock solution include an autolysis method, a pressure treatment method, and an enzymatic decomposition method. The yeast extract concentration is 20 to 55 w / w%, and the other solid content concentration is 10 to 20 w / w%. The production method is not particularly limited as long as a yeast extract stock solution having a total solid content of 30 to 75 w / w% can be obtained. As the filtration method, in addition to the diatomaceous earth filtration described above, membrane filtration, ultrafiltration, microfiltration and the like can be used.

【0006】[0006]

【実施例】以下に実施例を示すが、本発明はこれに限定
されるものではない。
EXAMPLES Examples will be shown below, but the present invention is not limited to these examples.

【0007】実施例1 酵母エキス原液の調製 ビール酵母から従来の方法で自己消化後、塩酸でpH
5.0に調製し、酵母エキス濃度が50w/w%になる
まで加熱濃縮し、塩濃度が15w/w%になるように食
塩を添加して、酵母エキス原液を得た。
Example 1 Preparation of a stock solution of yeast extract After autolysis of brewer's yeast by a conventional method, the pH was adjusted with hydrochloric acid.
The mixture was adjusted to 5.0, concentrated by heating until the yeast extract concentration became 50 w / w%, and salt was added so that the salt concentration became 15 w / w% to obtain a yeast extract stock solution.

【0008】実施例A 酵母エキス原液を135℃、1分間加熱処理後、0〜5
℃で原液が凍らないように7日間保った。その後、原液
を5℃以下に保ったまま、濾過率1.0(darcy)
の濾過助剤(ハイフロースーパーセル)を原液に対し
て、3w/w%添加して、濾過圧力2kg/cm2で濾
過を行い、酵母エキスを得た。
Example A After heat-treating a yeast extract stock solution at 135 ° C. for 1 minute,
The stock solution was kept at 7 ° C. for 7 days so as not to freeze. Thereafter, while keeping the stock solution at 5 ° C. or lower, the filtration rate is 1.0 (darcy).
Was added to the stock solution at 3 w / w%, and filtration was performed at a filtration pressure of 2 kg / cm 2 to obtain a yeast extract.

【0009】実施例B 酵母エキス原液を熱処理しないで、実施例Aと同様に0
〜5℃で原液が凍らないように7日間保持した後、濾過
を行い、酵母エキスを得た。
Example B The same procedure as in Example A was carried out except that the undiluted yeast extract solution was not heat-treated.
After keeping the stock solution at 液 5 ° C. for 7 days so as not to freeze, filtration was performed to obtain a yeast extract.

【0010】対照例 酵母エキス原液を135℃、1分間加熱処理後、0〜5
℃で原液が凍らないように1日間保った。以下、実施例
Aと同様に、濾過を行い、酵母エキスを得た。得られた
酵母エキス、実施例A,B,対照例および酵母エキス原
液について、各温度下での清澄性及び濾過能力、ケーク
比抵抗、粘度について比較したものを表1〜3に示す。
Control Example After heating the undiluted yeast extract at 135 ° C. for 1 minute,
The stock solution was kept at 1 ° C. for one day so as not to freeze. Thereafter, filtration was performed in the same manner as in Example A to obtain a yeast extract. Tables 1 to 3 show comparisons of the obtained yeast extract, Examples A and B, control examples, and undiluted yeast extract in terms of clarity and filtration ability, cake specific resistance, and viscosity at each temperature.

【0011】[0011]

【表1】 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 酵母エキス温度(℃) 実施例A 実施例B 対照例 酵母エキス原液 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 5 − − + + 20 − − + + 37 − − + + ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ −;清澄 ±;やや濁りあり +;濁りありTable 1 エ キ ス Yeast extract temperature (° C) Example A Example B Control Example Yeast extract stock solution 55 − − + + 20 − − + +37 − − + ++ − −; clarification ±; slightly turbid + ; Cloudy

【0012】[0012]

【表2】 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 実施例A 実施例B 対照例 酵母エキス原液 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 濾過処理能力(kg/8hr) 4,500 570 4,500 * ケーク比抵抗 3.49×108 1.11×1011 3.49×108 * ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 濾過処理能力:濾過面積15m2の場合の数値である。 *酵母エキス原液については、濾過効率が悪いので一般
に濾過は行わない。 ケーク比抵抗とは、単位濾過面積あたりのケーク(不溶
性成分)の与える抵抗のことであり、数字が小さいほど
濾過性が優れている。
2 Example A Example B Example Control Yeast extract Stock solution ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Filtration capacity (kg / 8hr) 4,500 570 4,500 * Cake Specific resistance 3.49 × 10 8 1.11 × 10 11 3.49 × 10 8 * ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ ━ Filtration capacity: It is a numerical value when the filtration area is 15 m 2 . * Filtration is not generally performed on the undiluted yeast extract because of its poor filtration efficiency. The cake specific resistance is the resistance given by cake (insoluble component) per unit filtration area, and the smaller the number, the better the filterability.

【0013】[0013]

【表3】 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 実施例A 実施例B 対照例 酵母エキス原液 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 粘度(cps) 18 19 24 1250 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 粘度の測定温度:35℃Table 3 A Example A Example B Control Example Yeast extract stock solution粘度 Viscosity (cps) 18 19 24 1250 ━━━━━━━測定 Measurement temperature of viscosity: 35 ℃

【0014】以上の結果から、酵母原液を1週間冷却保
持後、濾過を行うことにより、清澄で流動性の良い酵母
エキスが得られることが確認された。さらに、冷却する
前に加熱処理の工程を追加することで、濾過処理能力が
高まり、ケーク比抵抗が低くなることから、効率良く高
濃度の酵母エキスが得られることがわかった。
From the above results, it was confirmed that the yeast stock solution was cooled and held for one week, and then filtered to obtain a clear yeast extract having good fluidity. Furthermore, it was found that by adding a heat treatment step before cooling, the filtration capacity was increased and the cake specific resistance was reduced, so that a high-concentration yeast extract could be obtained efficiently.

【0015】実施例2 実施例Aで得た酵母エキスを、5℃、1週間冷却保存し
たもの(実施例C)、5℃、1ヶ月冷却保存したもの
(実施例D)、20℃、1週間保存したもの(実施例
E)について、清澄性、粘度について比較したデータを
表4に示す。
Example 2 The yeast extract obtained in Example A was stored by cooling at 5 ° C. for 1 week (Example C), stored at 5 ° C. for 1 month (Example D), 20 ° C., Table 4 shows data comparing the clarity and the viscosity of the sample stored for a week (Example E).

【表4】 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 実施例A 実施例C 実施例D 実施例E ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 清澄性 − − − − 粘度(cps) 18 18 18 18 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ −;清澄 ±;やや濁りあり +;濁りあり 以上の結果から、実施例Aの酵母エキスを冷蔵または室
温保存しても、清澄性、粘度が変わらないことから、本
発明で得られた酵母エキスは、冷蔵ないし室温下で清澄
で、流動性が良いことが確認された。
[Table 4] Example A Example C Example D Example E ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Clarity----Viscosity (cps) 18 18 18 18 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ −; clarity ±; slightly turbid +; turbid Therefore, even if the yeast extract of Example A is refrigerated or stored at room temperature, the clarity and viscosity do not change, so that the yeast extract obtained in the present invention is clear at refrigeration or room temperature and has good fluidity. Was confirmed.

【0016】[0016]

【発明の効果】本発明の酵母エキスは、酵母エキスが高
濃度含有されているのにもかかわらず、清澄で流動性が
よいことから、取り扱いやすくなり、酵母エキスの調味
料としての利用が拡大した。更に、製造工程を工夫する
ことで、経済性が良く、工業レベルでの製造に優れてい
る製造法を提供することができた。
EFFECT OF THE INVENTION The yeast extract of the present invention is clear and has good fluidity despite its high concentration, which makes it easy to handle and expands the use of yeast extract as a seasoning. did. Further, by devising the manufacturing process, it was possible to provide a manufacturing method which is economical and excellent in manufacturing at an industrial level.

Claims (2)

(57)【特許請求の範囲】(57) [Claims] 【請求項1】 酵母エキスの総固形分濃度が30〜75
w/w%、冷蔵ないし室温保存下で清澄で、粘度が10
〜1000cpsと流動性がよいことを特徴とする酵母
エキス。
1. The yeast extract having a total solid content concentration of 30 to 75.
w / w%, clear under refrigerated or room temperature storage,
A yeast extract characterized by having good fluidity of up to 1000 cps.
【請求項2】 酵母エキス原液を、−18〜5℃の凍ら
ない条件下に7日〜30日間保持した後、濾過を行うこ
とを特徴とする請求項1記載の酵母エキスの製造法。
2. The method for producing a yeast extract according to claim 1, wherein the yeast extract stock solution is kept at -18 to 5 ° C. under non-freezing conditions for 7 to 30 days, and then filtered.
JP5215061A 1993-08-06 1993-08-06 Yeast extract and its production method Expired - Fee Related JP2641161B2 (en)

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Application Number Priority Date Filing Date Title
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JP2641161B2 true JP2641161B2 (en) 1997-08-13

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007222078A (en) * 2006-02-23 2007-09-06 Sanei Gen Ffi Inc Method for purifying yeast extract
JP4833940B2 (en) * 2007-08-22 2011-12-07 アサヒビール株式会社 Yeast feeder

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