JP2590440B2 - Shrimp glass sprout sex attractant - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a sex attractant for use in controlling shrimp murmurs belonging to the order Lepidoptera, Pteridophyta, or for investigating the developmental fate.

[0002]

Background Art Shrimp glass sparrow is an insect belonging to the order Lepidoptera and is an agricultural crop pest that eats crops such as sweet potato. Up to now, pest control has been mainly carried out by spraying pesticides, but recently there has been a demand for the development of control methods that have little adverse effect on the environment, and pest control systems that rely solely on pesticides. Therefore, there is a need for the development of new control methods using behavior control substances and natural enemies.

[0003] Recently, the chemical structure of sex pheromones has been elucidated for many pests, and it has become possible to efficiently carry out investigations on the occurrence of pests using attractive sex pheromones. Using sex attractants,
Methods are being developed to control pests by capturing large amounts of adult males or disrupting male and female mating behavior.

[0004] In view of these circumstances, the present inventors have studied the sex pheromone of shrimp glass sparrows belonging to the order Lepidoptera of the Lepidoptera, isolated a substance having high attraction to males from female adult shrimp glass sparrows, and Chemical synthesis was attempted, and it was found that the obtained compound showed an effective attraction effect on male shrimp glass sparrows. The present invention has been completed based on these findings.

[0005]

DISCLOSURE OF THE INVENTION The present invention provides a sex attractant for shrimp viper, characterized by containing (E, E) -11,13-hexadecadienal as an active ingredient. Hereinafter, the present invention will be described in detail.

The active ingredient (E, E) -1 of the present invention
1,13-Hexadecadienal alone has a strong sexual attraction effect on male shrimp murmurs. This active ingredient
As it is, or dissolved in an organic solvent such as hexane to form a solution, which is adsorbed on a suitable carrier, for example, plastic or rubber such as polyethylene, or by encapsulation in a plastic capsule or capillary,
It can be a sustained-release preparation of the type that is gradually released into the air.

Next, the isolation and identification of the active substance of the sex attractant according to the present invention from a female adult and a test for confirming the biological activity of the active substance will be described in detail. (1) Separation and identification of active substance The tracking of the active substance was performed by an assay method using a gas chromatograph equipped with an antenna potential detector using a biopotential generated in a male antenna when an active substance was detected.

[0008] Adults were emerged from pupae obtained by breeding using artificial feed. Sex pheromone secretory glands near the abdominal terminal nodes of 20 female adults were cut out and extracted with hexane for 5 to 30 minutes. The extract was fractionated by column chromatography packed with florisil. Analysis of this 5% -ether / hexane fraction using a gas chromatograph equipped with an antennal potential detector revealed that there was one component that generated a clear potential in the male antennae of the shrimp porpoise. When this fraction was analyzed using a gas chromatograph-coupled mass spectrometer (GC-MS), the mass spectrum showed that the active substance was an unsaturated aliphatic aldehyde having 16 carbon atoms. The mass spectrum of this active substance is shown in FIG.

The mass spectrum of this active substance is shown by A
ndo et. al. [Agricultural a
nd Biological Chemistry Vol. 52, No. 6, pages 1415-1423, (1988)]
As a result of analysis based on the report by
It was found that the compound had a double bond at each of the 11th and 13th positions. From these analytical results, the active substance was estimated to be 11,13-hexadecadienal. Then, 11,13-hexadecadienal was separately synthesized, and the four isomers were subjected to gas chromatographic analysis. As a result, the active substance was identical to the (E, E) -isomer. Also, its mass spectrum was completely consistent with that of the active substance (FIG. 1). Based on the above findings, the above-mentioned active substance was identified as (E, E) -11,13-hexadecadienal.

(2) Biological activity test of active substance The attracting activity of the compound obtained by the above-mentioned chemical synthesis on adult males was examined using an indoor wind tunnel. A predetermined amount of the chemically synthesized compound was contained in a filter paper piece of 4 cm in length and 1 cm in width and suspended in a wind tunnel as an attraction source, and then male adults were released from downwind and their behavior was observed. When the compound was contained in an amount of 0.01 to 1 μg per piece of filter paper, the behavior of repeatedly attracting male moths was observed. Also, the approaching male, on a piece of filter paper containing the synthetic compound,
They were found to exhibit mating behavior such as opening the mating device and bending the abdomen.

[0011]

[Table 1]

As described above, a substance exhibiting a high sex-inducing activity for males of the prawn sparrow belonging to the order Lepidoptera of the Lepidoptera was isolated, and the chemical structure of the substance was determined by collation with the chemically synthesized compound. The sex attractant according to the present invention is used for the survey of the occurrence of shrimp glass sprout, and according to the occurrence of the shrimp glass sprout, it is effective for the effective use of the insecticide. It is very useful that it can be used for controlling shrimp murmurs by utilizing the property of sex attractant instead of controlling.

[Brief description of the drawings]

FIG. 1 is a mass spectrum diagram of an active substance of a sex attractant according to the present invention.

 ──────────────────────────────────────────────────続 き Continued on the front page (56) References AGRIC. BIOL. CHEM. 52, No. 6, pp. 1415-1423 (1988)

Claims (1)

(57) [Claims] 1. A sex attractant for shrimp glass sparrows comprising (E, E) -11,13-hexadecadienal as an active ingredient.