JP2548243B2 - Reverse pollution prevention device and reverse pollution prevention method using the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention completely prevents the invasion of microorganisms from the intake port of various aseptic water producing devices used in medical institutions such as hospitals, pharmaceutical manufacturers, food manufacturers, etc. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a reverse pollution prevention device and a reverse pollution prevention method using the same.

[Conventional technology and its problems]

It is well known that the non-incorporated water that does not contain impurities such as fine particles and microorganisms such as bacteria is essential for medical institutions such as hospitals and medical product manufacturers as well as food manufacturers.

In recent years, a filtration membrane capable of removing microorganisms such as bacteria has been developed, and a sterile water producing apparatus which can relatively easily produce sterile water and has high reliability has been put into practical use. However, the inside of the water conduit leading to the aseptic water producing device and the intake port may be contaminated by microorganisms such as bacteria suspended in the air, and it has been a long-standing problem to prevent contamination by such microorganisms.

Heretofore, the following four methods have been mainly proposed as methods for controlling the contamination of the inside of the water pipes leading to the sterile water production apparatus and the water intake port with microorganisms.

(1) At the same time as the passage of aseptic water from the aseptic water production equipment is stopped, the heater installed in the conduit is heated to heat the water inside the conduit and release it as water vapor to the outside through the invasion route of bacteria. By drying the inside of a certain water pipe, the growth of bacteria is blocked, and the heater is turned off after a predetermined time has elapsed by a timer, and the inside of the water pipe becomes negative pressure as the temperature inside the water pipe decreases. A heat sterilization method that seals the check valve provided by itself with its own weight and pressure to prevent bacteria from entering.

(2) A valve in which a slanted elliptical rib is integrally molded inside a cylindrical silicone rubber tube, and the elastic pinch has a function of blocking the rib from outside by pressing the rib from one direction or both directions. Elastic pinch valve system that uses a valve.

(3) A silver vapor deposition filling method in which a silver vapor deposit is filled in a faucet and bacteria are prevented from entering from the faucet by utilizing a sterilizing effect of silver ions.

(4) Disinfection method using a disinfectant such as sodium hypochlorite water.

However, with these methods, it is difficult to avoid microbial contamination at the tip of the faucet even if it is possible to prevent the invasion of microorganisms from the water conduit into the aseptic water production system. Therefore, at least the water that first flows out of the faucet cannot be completely sterile water.

The present inventors previously comprised an inorganic halide supply device, an electrolysis device, and a water shutoff valve provided at the intake, and stopped the sterile water obtained by passing raw water through the sterile water production device. After the water valve is closed to store water in the electrolysis chamber of the electrolysis device, inorganic halide is supplied from the inorganic halide supply device into the electrolysis chamber and electrolyzed to sterilize the interior of the electrolysis chamber and the water conduit. A new anti-contamination preventing device was newly developed and proposed as Japanese Patent Application No. 61-107340 (Japanese Patent Application Laid-Open No. 62-262787).

This device makes it possible to disinfect from the water conduit to the intake by generating hypohalite by electrolysis and causing water containing this hypohalite to flow out from the faucet. is there.

As a result of further research conducted by the present inventor, the same effect can be easily achieved without providing a water shutoff valve at the water intake side by providing a water shutoff valve at the water intake side and / or the opposite side of the sterile water manufacturing apparatus. The inventors have reached the present invention.

[Means for solving problems]

That is, the present invention is a reverse pollution preventing device for a sterile water manufacturing apparatus, wherein an electrolytic device is provided between the water intake and the sterile water manufacturing apparatus, and a stop provided on the intake side of the sterile water manufacturing apparatus and / or on the opposite side thereof. After closing the water valve to stop the supply of aseptic water, the inorganic halide is supplied into the electrolysis chamber of the electrolysis apparatus in a state where the sterile water is stored in the electrolysis chamber of the electrolysis apparatus, and the electrolysis chamber and the conduit tube are electrolyzed. Is a reverse pollution preventing device characterized by being sterilized. Further, a second invention is to provide an electrolysis device between the water intake and the sterile water producing device, and to Or, after closing the water shutoff valve provided on the opposite side to stop the supply of aseptic water, while the aseptic water is stored in the electrolysis chamber of the electrolyzer, the inorganic halide is supplied to the electrolysis chamber for electrolysis. By the hypohalite generated by Sterilizing the inside of the electrolysis chamber and the water pipe, and further, by the pressure of the gas generated from the electrode in the electrolysis chamber, the hypohalite-containing water is made to flow toward the water intake port, and the water pipe and the water intake port to the water intake port are sterilized. It is a method for preventing reverse pollution, which is characterized by

[Action]

The anti-contamination prevention device of the present invention is a halide supply device,
It is composed of a water shutoff valve provided on the side of the electrolysis device and the sterile water producing device and / or on the opposite side.

Inside the water conduit filled with aseptic water, if left as it is, there is a possibility of secondary contamination by microorganisms that have entered through the water intake. In the reverse pollution control device of the present invention, in order to prevent this secondary pollution, after the use of the sterile water is completed, the water shutoff valve provided on the intake side and / or the opposite side of the sterile water manufacturing device is closed to ensure the aseptic condition. After the supply of water is stopped, hypohalite is generated or injected into the water conduit with sterile water stored in the electrolysis chamber of the electrolyzer. For the generation of hypohalite, for example, after injecting an inorganic halide from an inorganic halide supply device into the stored water of a water conduit, the electrolysis device is energized and the stored water containing the aqueous halide solution is electrolyzed. When the stored water is electrolyzed, the halogen ions contained in the stored water are converted into halogen molecules by anodic oxidation, and the halogen molecules are further reacted as hydroxides to generate hypohalite.

When sodium chloride, for example, is used as the inorganic halide, this series of reactions is shown by the reaction formulas as follows.

The thus-produced hypochlorite is effective against most microorganisms such as viruses, general asporosis bacteria, acid-fast bacteria, bacterial spores, filamentous fungi, algae, protozoa, etc.
It has the ability to complete sterilization in a short time at a concentration of ppm.

This sodium hypochlorite sterilizes the water stored in the electrolysis chamber and at the same time sterilizes the water conduit.

Further, gas such as hydrogen gas is generated from the electrode by electrolysis and fills the space between the sterile water producing device and the electrolysis chamber.
Due to the filled gas pressure, the hypohalite-containing water gradually flows to the water intake port, whereby the water intake port is sterilized and the reverse pollution prevention effect is enhanced.

In addition, the disinfectant-containing water is stored in relation to the negative pressure in the water conduit only when the intake port is composed of pores with a diameter of 2.5 mm or less. Therefore, not only is there a possibility that the inside of the conduit cannot be contaminated by microorganisms when the device is not used, such as at night, but also the intake can prevent secondary contamination of microorganisms by the action of the disinfectant-containing water stored in the intake. Therefore, it is more preferable.

〔Example〕

One embodiment of the present invention will be described with reference to FIG. In FIG. 1, (1) is an aseptic water production device, (2) is an inorganic halide supply device, (3) is an electrolysis device, (4) is a water stop valve on the side opposite to the water intake, and (5) is water intake. Mouth stop valve,
(6) is an intake, and (10) is a water conduit. Examples of the aseptic water production device (1) include a distilled water production device, a pure water production device, an ultraviolet sterilization device, an ultrafiltration device, a reverse osmosis device, and a heat sterilization device which are usually used, but not limited to these. 1 μm vertical and 0.1 μm wide on the surface of hollow fiber
A module composed of a polyethylene porous hollow fiber membrane having slit-shaped ultrafine pores, such as Stella Pore (registered trademark, manufactured by Mitsubishi Rayon Co., Ltd.) can be used.

In addition, it goes without saying that a filtration module using a hollow fiber membrane or a flat membrane of polyacrylonitrile, polysulfone, polyvinyl alcohol, polymethyl methacrylate, etc. other than this can also be used.

As the aseptic water producing apparatus (1), an apparatus using a porous hollow fiber is more preferable because of its simple structure and low cost.

The water stop valve has a function of stopping the raw water and a function of closing the aseptic water production apparatus in order to fill the gas generated by electrolysis, but its installation location differs depending on the type of the aseptic water production apparatus. For example, when using an aseptic water production system including a porous hollow fiber module, at least a water shutoff valve (4) on the side opposite to the water intake is provided. When using other aseptic water production equipment, at least the water stop valve (5) on the intake side is provided. However, when the aseptic water manufacturing apparatus becomes large, both the water shutoff valves (4) and (5) may be provided.

FIG. 2 shows an apparatus for producing aseptic water using a polyethylene porous hollow fiber, (7) is an electrode, (8) is a porous hollow fiber, and (9) is an air vent.

The polyethylene porous hollow fiber is previously subjected to a hydrophilizing treatment, but the above-mentioned membranes other than this are kept hydrophilic even if they are not particularly hydrophilized. These hydrophilic membranes have a property of allowing water to permeate therethrough but not allowing water-insoluble gas to permeate therethrough.

Aseptic water produced by the aseptic water production apparatus (1) is taken in through the intake port (6), but when the use of the aseptic water is completed, the water shutoff valve (4) is closed to stop the supply. At this time, the raw water in the sterile water production device (1) is filtered through the hollow fiber membrane (8) by the atmospheric pressure of the air entering from the air vent (9),
It is dripped gradually from the water intake (6). According to this, the water surface of the raw water in the sterile water production device (1) gradually lowers, but since the hollow fiber (8) does not allow air to permeate, after a certain amount of sterile water has dropped due to negative pressure, the inside of the electrolyte (31) And remains in the water conduit between the aseptic water production system (1) and the electrolysis chamber (3). Here, it is more preferable to set the position of the water intake (6) higher than the electrolysis chamber (31) because the amount of water stored in the electrolysis chamber (31) and the water conduits before and after the electrolysis chamber (31) increases.

The inorganic halide is supplied from the inorganic halide supply device (2) to the sterile water in the electrolysis chamber (31).

As the inorganic halide supply device (2), for example, an inorganic halide aqueous solution supply device having a structure as shown in FIG. 3 can be used.

That is, the bellows type inorganic halide aqueous solution container (22) stably installed by the container stabilizing rod (21) is mounted in the main body of the inorganic halide aqueous solution,
The pressing plate (26) is moved by a certain distance by the feed screw (25) by turning the feed handle (23) for supplying the inorganic halide aqueous solution. By this operation, a fixed amount of the inorganic halide aqueous solution in the inorganic halide aqueous solution container (22) is injected into the electrolytic chamber (31) shown in FIG. 4 through the opening (36). Further, the solution in the electrolysis chamber (31) is prevented from flowing back into the inorganic halide aqueous solution container (22) and the concentration of the inorganic halide aqueous solution in the inorganic halide aqueous solution container (22) is changed and generated. A check valve (24) or the like may be provided at the boundary between the electrolytic chamber (31) and the inorganic halide aqueous solution container (22) in order to prevent gas from entering.

As the inorganic halide aqueous solution container (22), plastic containers having various shapes other than a bellows shape can be applied. As an example, it is possible to apply a pinchable polyethylene bottle or the like, but in this case, it is necessary to provide an air valve in order to restore the container shape to the original shape after supplying the inorganic halide aqueous solution. preferable. Alternatively, a method of using a container having a mechanism such as a syringe and injecting a fixed amount of the inorganic halide aqueous solution by pushing a piston for a fixed length may be adopted.

The concentration and amount of the aqueous inorganic halide solution to be injected into the electrolysis device (3) are determined by the electrolysis chamber (31) in the electrolysis device (3).
Since it depends on the amount of stored sterile water, etc., it cannot be unconditionally determined, but usually about 10 to 40 ml of sterile water in the electrolysis chamber and the conduit tube is used as an inorganic halide aqueous solution with a concentration of about 0.1 to about 30%. Used by injecting about 0.1 to 5.0 ml.

In the present invention, as the inorganic halide aqueous solution that can be used, NaCl, KCl, LiCl, AlCl ₃ , NH ₄ Cl, CaCl ₂ or the like, an aqueous solution of an inorganic chloride that produces chlorine ions by electrolysis, NaI, KI, etc. An aqueous solution of inorganic iodide, N
Examples thereof include aqueous solutions of inorganic bromides such as aBr and KBr. Among them, NaCl is preferably used from the viewpoint of easy availability.

The inorganic halide aqueous solution may be injected into the electrolytic chamber from the inorganic halide aqueous solution container using a metering pump.

Further, in the present invention, not only the method of injecting the inorganic halide aqueous solution into the electrolysis chamber (31) as described above,
For example, it is also possible to adopt a method of supplying a predetermined amount to the electrolytic chamber (31) using an inorganic halide exhibiting a sustained release property such as tablets or capsules formed into pellets. As the tablet or the like, a sustained release tablet in which the component is coated with hydrogel or the like may be used so that the component is gradually eluted. A crosslinked 2-hydroxyethyl methacrylate polymer or copolymer is preferably used as the hydrogel.

Further, a system may be used in which a sustained-release tablet is set in the vicinity of the electrode and is periodically replaced depending on the life of the tablet.

The inorganic halide supplied to the electrolysis chamber (31) mixes with sterile water in the electrolysis chamber (31) and is then electrolyzed by energizing the electrodes.

That is, in FIG. 4, an anode (32) and a cathode (33) are provided in the lower part of the electrolysis chamber (31), and a power source (34)
Direct current electricity from is supplied.

The electrodes used for the anode (32) and the cathode (33) are usually platinum electrodes that do not undergo changes such as corrosion even when immersed in water for a long period of time, metals such as copper and nickel, or synthetic resins, ceramics, etc. Also used are electrodes that have been plated or vapor-deposited with gold or platinum. Further, in order to facilitate the exchange of these electrodes, the cap (35) provided with the electrodes may be fixed by screws as shown in FIG.

As the power source (34), it is possible to use a household AC electricity (100V) transformed into a direct current by a transformer or a battery such as a dry battery. Further, a timer may be installed between the power source (34) and the electrode so as to energize at a predetermined time.

The voltage and current applied to the anode (32) and the cathode (33) and the energization time cannot be unconditionally determined because they vary depending on the type, concentration and amount of the inorganic halide aqueous solution in the electrolysis chamber (31). However, when the inorganic halide is NaCl, for example, the effective chlorine concentration in the electrolysis chamber (31) is usually adjusted to 0.5 to 500 ppm, especially about 10 ppm or more for sterilizing microorganisms such as mold. To be done. The electrolysis time with the aqueous sodium chloride solution varies depending on the concentration of the aqueous sodium chloride solution, the supply amount, the current amount, etc., but is preferably 10 to 120 minutes.

In the apparatus of FIG. 2, by injecting an inorganic halogen derivative into the electrolyzer (3) and then electrolyzing it, hypohalite is produced and at the same time, a gas such as hydrogen is produced from the electrode.

Then, since the porous hollow fiber (8) does not pass the gas which is hardly soluble in water, the generated gas which is hardly soluble in water is blocked and filled in the direction of the aseptic water producing device (1). The hypohalite-containing water in the electrolyzer (3) is pushed toward the inside of the water intake (6) and gradually flows out. The generation of the flow of hypohalite-containing water by such gas pressure can disinfect between the electrolytic chamber and the water intake.

Further, as shown in FIG. 5, the water intake (6) has a single or a plurality of pores (61) having a diameter of 2.5 mm or less in the faucet nozzle (60).
In the case of a water intake system, part of the water containing hypohalite that flows from the electrolyzer to the intake due to gas pressure is stored in the intake, which completely prevents the reverse contamination of microorganisms from the intake. can do.

The faucet nozzle (60) can be attached to and detached from the water conduit (5) by screws, fitting means, etc., so that the faucet nozzle can be easily washed or replaced.

The pores (61) have a diameter of 2.5 mm or less, and are provided in a single number or in a plurality. If the diameter is larger than 2.5 mm, the disinfectant-containing water completely flows out from the intake port and is not stored at all, or only slightly stored, so that the object of the present invention cannot be sufficiently achieved.

FIG. 6 shows an embodiment of the present invention by an aseptic water producing apparatus using the porous hollow fiber modules (48) (49). Tap water is supplied from (411) by opening the solenoid valve (410), filtered by the porous hollow fiber modules (48) (49), and sterile water flows out from the water intake (41). At the end of use of sterile water, close the solenoid valve (410). At this time, the water pipe (4
3), the electrolysis chamber (44) is filled with sterile water. Next, after turning the handle (47) to supply the inorganic halide-containing water (46) to the electrolysis chamber (44), the electrode (45) is energized to perform electrolysis for a certain period of time. As a result, the hypohalite is dissolved in the water conduit (43) in and around the electrolysis chamber. The water in the electrolysis chamber (44) and the water conduit (43) gradually flows out of the water intake port (41) during the electrolysis and after the electrolysis due to the pressure of the gas generated by the electrolysis. Intake (41)
Is composed of pores having a pore diameter of 2.5 mm or less, and the hypohalite-containing water is stored in both the water guide pipe (43) and the intake port (41) to completely prevent secondary contamination by microorganisms.

Note that (412) is a control unit for automatically performing the above operation, and (50) connects the control unit (412) to the solenoid valve (410), the control unit (412) to the electrode (45). It is an electric cord.

7 and 8 show another embodiment of the present invention.
(51) is an intake port consisting of pores with a diameter of 2.5 mm or less, (54)
Is an aseptic water production system using a reverse osmosis membrane. Since a sterile water production system using a reverse osmosis membrane is susceptible to secondary contamination by microorganisms, a small sterilization filter (52) is provided as shown in Fig. 7, but it is not necessary to provide it as shown in Fig. 8. Good.
The operation and effect are almost the same as those of the apparatus shown in FIG.

Next, FIG. 1 shows an example in which a reverse osmosis membrane device is used as the sterile water production device (1). In this case, the stop valve (5)
Is installed and the water shutoff valve (5) is closed at the end of use of sterile water. At this time, a part of the aseptic water between the water shutoff valve (5) and the water intake port (6) flows out from the water intake port (6), but is generated in the water conduit between the water shutoff valve (5) and the electrolysis chamber. Due to the negative pressure, all of it remains in the electrolytic chamber without flowing out. Therefore, by injecting an inorganic halide from the inorganic halide supply device (2) and performing electrolysis, the same effect as in the case of the hollow fiber module can be obtained. Further, since the water is blocked by the water shutoff valve (5), the hypohalite-containing water flows to the water intake port (6) by the generated gas pressure to prevent secondary pollution.

Even in this case, it is preferable to place the water intake higher than the electrolysis chamber, especially higher than the water shutoff valve (3) because the amount of water stored in the electrolysis chamber and the water conduit increases.

[Example 1] In the apparatus shown in Fig. 1, Steapore (manufactured by Mitsubishi Rayon Co., Ltd.) was used as an apparatus for producing sterile water.

Then inoculate 0.5 ml of 30 ml of sterile water in the conduit with the inoculum cultivated by the method detailed below, and inoculate the inoculum into the electrolysis chamber with a syringe (for 1 ml) of 10 ⁵ to 10 ⁶ It was inoculated so that it would be / ml. Next, 0.5 ml of a 10% sodium chloride aqueous solution was injected into the electrolytic chamber by an inorganic halide supply device, a direct current of 10 mA was applied to the two electrodes, and electrolysis was performed for 60 minutes.

After the electrolysis is completed, the water containing sodium hypochlorite is allowed to flow out, and then sterile water is passed from the sterile water manufacturing device and taken from the tap, and the 10th revised Japanese Pharmacopoeia “General test method 37. Sterility test method” The sterility test was carried out based on Judgment was carried out by culturing at 30 ° C. for 7 days in the recent test and at 20 ° C. for 10 days in the fungal test. Table 2 shows the results.

 The inoculum was adjusted by the following method.

(Preparation of inoculum) Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger are used as inoculum.
E. coli, Staphylococcus aureus, and Pseudomonas aeruginosa were cultured at 35 ° C for 24 hours and then passed for 3 consecutive passages for 3 consecutive passages. Candida albicans
After culturing at 25 ° C for 48 hours, the cells were subcultured every 2 days for 2 passages, and Aspergillus niger was cultured at 25 ° C for 1 week.

E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida
Albicans was washed three times with a sterilized physiological saline in a centrifuge at 3000 rpm for 10 minutes, and then used as a bacterial solution having a bacterial count of 10 ⁸ / ml.

For Aspergillus niger, a spore suspension was prepared using sterile physiological saline containing 0.05% polysorbate 80, washed with a centrifuge three times at 3000 rpm for 10 minutes, and then resuspended in sterile physiological saline. , 10 ⁷ cells / ml.

From the above results, it was found that the secondary pollution between the water outlet and the water intake of the aseptic water production system is completely prevented by the reverse pollution control system of the present invention.

[Example 2] In the apparatus shown in Fig. 7, the solenoid valve (410) was opened, tap water was injected into the sterile water producing apparatus to produce sterile water, and the sterile water was fed into the electrolysis chamber with an internal volume of 30 ml. Sterile water was stored in the electrolysis chamber.

Next, 1 ml of a 10% sodium chloride aqueous solution was injected into the electrolysis chamber, two electrodes were energized, and the chlorine concentration in the electrolysis chamber after 60 minutes had elapsed was measured to find an effective chlorine concentration of 194 ppm. Due to the gas pressure generated by the electrolysis, the sodium hypochlorite-containing water in the electrolysis chamber gradually flowed out from the intake. When 120 minutes passed after the completion of electrolysis, the effective chlorine concentration of the water stored in the intake port (41) was measured and found to be 4 ppm.

[Example 3] Escherichia coli IFO3972 and Serratia were used as test bacteria.
marcescens IFO12648 was cultured at 35 ° C. for 24 hours, and the cells were passaged for 3 consecutive days for 3 consecutive days. Then, using a sterilized physiological saline, the cells are washed three times at 300 rpm for 10 minutes with a centrifuge to prepare a bacterial solution of 10 ⁸ cells / ml.

After adjusting the test bacterial solution (10 ⁸ cells / ml), sterilized physiological saline 3
1 ml of the test bacterial solution was inoculated to 00 ml to make the viable cell count in physiological saline 10 ² cells / ml.

After using aseptic water in the same apparatus as in Example 1, electrolysis was performed in the same manner as in Example 1 to prepare a test bacterial solution prepared with 20 ml of sodium hypochlorite-containing water stored in the water intake (41). Contacted. Water stored at the intake after being left for 5 days
When the viable cell count in 20 ml was measured, E. Coli and S. marcesce
Both ns were 0. From this result, it is clear that microbial contamination from the intake can be completely prevented.

〔The invention's effect〕

As described above, according to the reverse pollution preventing device of the present invention, it is possible to surely sterilize the inside of the water conduit from the sterile water manufacturing device to the water intake to prevent reverse pollution, and further its structure is simple and handling. Is extremely simple. In particular, since a water shutoff valve is not required between the electrolyzer and the water intake, the cost is low, and the system for automation can be simplified.

[Brief description of drawings]

FIG. 1 is an explanatory view of a reverse pollution preventing device of the present invention, FIG. 2 shows an example in which the present invention is applied to an aseptic water producing device comprising a porous hollow fiber module, and FIG. 3 is an inorganic halide supply. FIG. 4 is a schematic explanatory view of the apparatus, FIG. 4 is a schematic explanatory view of the electrolysis apparatus, FIG. 5 is a perspective view of an intake port, and FIG. 6 is an example in which the present invention is applied to an aseptic water production apparatus including a porous hollow fiber module. FIG. 7 and FIG. 8 show an example in which the present invention is applied to a reverse osmosis membrane aseptic water producing apparatus. (Main symbols in the drawing) 1: Aseptic water production device 2: Inorganic halide supply device 3: Electrolysis device 4: Water stop valve 5: Water stop valve 6: Water intake valve 10: Water pipe

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification number Internal reference number FI Technical indication C02F 1/50 531 C02F 1/50 531K 531P 540 540B 550 550D 560 560E 560F (72) Inventor Kazuo Shimizu 2-11-33, Noritake Shinmachi, Nishi-ku, Nagoya-shi, Aichi Tome Industries Co., Ltd. (72) Inventor Masahiro Morita 4-226-1, Higashi-Funao-cho, Hamadera, Sakai City, Osaka Prefecture

Claims (4)

(57) [Claims] 1. A reverse pollution preventing device for a sterile water manufacturing apparatus, wherein an electrolysis device is provided between the water intake and the sterile water manufacturing apparatus, and a stop provided on the intake side and / or the opposite side of the sterile water manufacturing apparatus. After the water valve is closed to stop the supply of aseptic water, the inorganic halide is supplied into the electrolysis chamber in a state where the sterile water is stored in the electrolysis chamber of the electrolysis apparatus, and the electrolysis chamber and the water conduit tube are electrolyzed. Reverse contamination prevention device characterized by sterilizing 2. The reverse pollution preventing device according to claim 1, wherein the water intake is located higher than the electrolytic chamber. 3. A single or a plurality of pores having a diameter of 2.5 mm or less are provided at the water intake, so that the hypohalite-containing water is caused to flow toward the water intake by the pressure of the gas generated from the electrode in the electrolysis chamber. The reverse pollution preventing device according to claim 1, wherein the hypohalite-containing water is stored at an intake. 4. An electrolyzer is provided between the aseptic water producing apparatus at the water intake and a water shutoff valve provided at the aseptic inlet side and / or the opposite side of the aseptic water producing apparatus is closed to supply the aseptic water. After stopping, aseptic water is stored in the electrolysis chamber of the electrolyzer.Supplying an inorganic halide in the electrolysis chamber, and sterilizing the electrolysis chamber and water conduit by hypohalite generated by electrolysis. In addition, the pressure of the gas generated from the electrode in the electrolysis chamber causes the water containing hypohalite to flow toward the water intake port, and sterilizes the water pipe inside the water intake port and the water intake port. Method.