JP2531969Y2 - Cell scraper - Google Patents

Description

[Detailed description of the invention]

[0001]

The present invention is used in the field of cell culture and tissue culture to remove cells and tissues from petri dishes and flasks when collecting cells and tissues cultured in petri dishes and flasks. It relates to a cell scraper.

[0002]

2. Description of the Related Art When a cell or tissue is detached from a petri dish, a flask or the like without using trypsin, a cell scraper is used. As a simple one, there is a handmade one in which a rubber piece is attached to a tip of a glass rod with a wire or the like, or a handmade one, but disposable cell scrapers are commercially available from several companies.

[0003] These disposable cell scrapers have been sterilized and have the advantage that they can be used immediately after being taken out of a sterile bag. However, they are disposable and must be stored in sterile bags one by one.
Since a large amount of cell scrapers must be packed in one cardboard case, the volume must be reduced as much as possible, and there are restrictions on the shape and structure. For this reason, most of the conventional disposable cell scrapers which are commercially available, as shown in FIG. 5, for example, a handle (11) to which a blade (13) for scraping cells and tissues is attached in the longitudinal direction and a blade is attached. Are on the same plane.

In such a structure, the scraper of the cell scraper is inserted into the flask from the mouth of the flask with the handle, and the handle (11) is swung right and left to scrape the cells and tissues. When scraping cells and tissues in this way,
The scraped cells and tissues will collect right and left in the flask. In addition, cells and tissues will adhere to both surfaces of the blade (13). Then, in order to collect the scraped cells / tissues, it is necessary to inject a medium or the like into the left and right sides of the flask and to both sides of the blade to wash out and collect. Therefore, it is inefficient to process a large number of samples. Further, in the case of a petri dish, in the method of shaking the petri dish left and right while holding the handle, the cells and tissues scraped off may be scattered outside the petri dish due to the elasticity of the handle, which is not preferable from the viewpoint of work environment safety and health.

[0005] A commercially available disposable cell scraper has a blade (a scraping portion) and a handle integrally formed.
Or it consists of two parts. Both the handle and the blade are made of plastic. If the blade is made of plastic, the material is hard, so that the blade and the tissue are greatly damaged when scraped. In addition, when the blade portion is made of a soft material such as rubber, the force is not transmitted unless the blade is made somewhat large and thick because of its softness, so that cells and tissues cannot be scraped. If the blade is too thick, it will be difficult to insert the scraper through the mouth of the flask. Even in the case of a petri dish, it becomes difficult to scrape one edge of the petri dish.

[0006]

The present invention has been made as a result of various studies in order to solve such a problem of the conventional commercially available disposable cell scraper. The purpose of the present invention is to solve the problem. Another object of the present invention is to provide a compact disposable cell scraper that can efficiently scrape and collect cells and tissues from a flask or a petri dish and does not damage cells and tissues.

[0007]

That is, the present invention comprises a scraping portion consisting of a blade and its support, and a handle for holding the blade. The blade is made of a rubber-like elastic material, and the support and the handle are made of rubber. Along with being made of plastic, the scraping portion has a blade passing direction perpendicular to the long axis direction of the handle, and the blade surface is inclined at an angle of 30 to 70 degrees with respect to the long axis direction of the handle, It is attached to the tip of the handle, and with the projection or through hole of the attachment portion as the axis, it is possible to rotate 90 degrees to the left and right in a plane perpendicular to the long axis direction of the handle. It is a cell scraper which is a feature.

Hereinafter, the present invention will be described in detail with reference to the drawings. 1 and 2 are general views of a cell scraper according to an embodiment of the present invention. FIG. 3 shows an example of a structure of a tip portion of a handle, and FIG. 4 shows an example of a structure of a blade and its support. is there.

The handle (1) and the support (2) of the blade are plastic moldings, and the material is not particularly specified as long as it has a certain level of strength. Examples thereof include ABS resin, hard vinyl chloride resin, polypropylene, Examples include polyethylene, polystyrene, polyester, and TPX resin. The blade (3) is made of a rubber-like elastic material, and is not particularly specified unless it has cytotoxicity. Examples thereof include silicone rubber and viton rubber.

The handle (1) has a rod-like shape as a whole, and the tip of the blade to which the support (2) is attached is tapered, and further has a support (2) at the tip. ) Is provided with a plate-shaped mounting portion (4). Mounting part (4) is 5-15mm long
The surface is inclined at an angle of 30 to 70 degrees with respect to the long axis direction of the plate (1), and the blade (3) and the support (2)
A scraping part made of is designed to be attached at that angle. The inclination angle of the scraping portion is desirably 30 to 70 degrees. If the inclination angle is less than 30 degrees, the angle of the blade (3) with respect to the surface from which the tissue and cells are scraped is insufficient, and the cells and tissues cannot be scraped well. On the other hand, if it is larger than 70 degrees, it becomes difficult to insert the blade portion into the flask when the width of the blade (3) is larger than the diameter of the mouth of the flask.

In order to improve the operability when using a flask having a large culture area, as shown in FIG.
If the handle (1) is bent at the tip and at a position of 30 to 80 mm from the tip, it becomes easier for the blade to reach the four corners of the flask. The angle of bending is preferably in the range of 15 to 30 degrees from the viewpoint of operability.

As shown in FIGS. 3 (a) to 3 (c), the plate-like mounting portion (4) is provided with a projection (5) for mounting the support (2) on one or both sides thereof. Alternatively, as shown in FIG. 3D, the mounting portion (4) is composed of two plates, and a circular through hole (6) is provided therein.

On the other hand, on the top of the support (2),
As shown in FIGS. 4 (a) and 4 (b) (e and f are corresponding side views), the top is composed of two plates, or FIG. 4 (c) (g is the corresponding side view). 4), a notch (7) is formed in one plate as shown in FIG.
(D) As shown in (h is a corresponding side view), the top is formed of a single plate, and projections (8) are provided on both sides thereof.

FIGS. 4 (a) and 4 (b) for the mounting portion of FIG. 3 (a), and FIGS. 4 (c) and 3 (d) for FIGS. 3 (b) and 3 (c). 4), the supports shown in FIG. 4D are respectively combined, and the projections are fitted into the through holes or notches, so that the support (2) is attached to the tip mounting portion (4) of the handle (1). Attach. At this time the pattern (1)
And the support (2) are T-shaped, but the support (2)
With the projection (5) or the through hole (6) of the mounting portion (4) as an axis, the handle can be rotated left and right within a plane perpendicular to the long axis direction within a range of 90 degrees. Both are plastic fittings and can rotate smoothly.

The blade (3) is fixed to the support (2). The fixing method is as follows. A groove (9) is provided in the support as shown in FIG. May be inserted, or may be fixed by bonding as shown in FIG. Also, the pins may be fitted and fixed. The thickness of the blade (3) is from 1 to 3.5 mm, which is suitable for exhibiting softness characteristics as rubber. If the thickness is larger than this, insertion into the flask becomes difficult, and cells on one side of the petri dish or flask cannot be scraped off.

Next, the method of using the cell scraper of the present invention will be described. Since the product is stored in the sterilization bag, first, the cell scraper is removed from the sterilization bag.
(Hereinafter, the operation is performed by aseptic operation.) Next, the scraping portion composed of the blade (3) and the support (2) is
Rotate degrees. At this time, since the scraping portion is almost parallel to the handle (1), the blade portion can be put into the flask from the narrow mouth of the flask even if the scraping portion is somewhat long. Then, the cells are scraped off by repeatedly pulling the handle (1) forward while keeping the blade (3) in close contact with the culture surface of the flask. Since the blade (3) is made of a rubber-like elastic material and is soft and has good adhesion, the cell tissue can be reliably peeled off with a single passage of the blade (3), and damage to the cell tissue can be prevented. Absent.

Further, a support mounting portion (4) of the handle (1).
Since the angle is formed, the tip of the blade reaches the four corners of the flask by rotating the scraping part left or right or tilting the handle left and right. For this reason, the cell tissue can be reliably removed from the entire culture surface of the flask. After the cells have been scraped, wash the cells on the front side of the blade (3) with a medium or the like into the flask, and then rotate the scraper in the same manner as when the scraper was first placed in the flask to remove the cells. With the handle parallel, pull out the scraping part from the mouth of the flask. Thereafter, the medium is added to the flask, the cells in the scraped flask are washed out of the flask, and a series of operations for collecting the cells is completed.

When the cell scraper according to the present invention is marketed as a disposable product, it is contained in a sterile bag one by one. In this case, the scraper is rotated so that the scraper is rotated so that the handle and the handle are parallel to each other, so that the volume occupied by the cell scraper is minimized, and is within the same volume as a conventional commercial cell scraper. Even if a plurality of pieces are packed in a cardboard case, the volume can be kept small.

[0019]

[Effect of the Invention] According to the present invention, cells and tissues can be scraped and collected efficiently and reliably to every corner of a flask or petri dish without damaging cells or tissues. It can be kept small and is suitable as a disposable cell scraper.

[Brief description of the drawings]

FIG. 1 is an overall view of a cell scraper according to an embodiment of the present invention, wherein (a) is a perspective view and (b) is a side view.

FIG. 2 is an overall view of a cell scraper showing another embodiment of the present invention, wherein (a) is a perspective view and (b) is a side view.

FIG. 3 is a view showing a structure of a tip portion (attachment portion) of a handle of the cell scraper of the present invention.

FIG. 4 is a view showing the structure of a scraping portion (blade and support) of the cell scraper of the present invention, wherein (a) to (d) are front views and (e) to (h) are corresponding side views. It is.

FIG. 5 is an overall view showing an example of a conventional commercially available disposable cell scraper, wherein (a) is a perspective view and (b) is a side view.

[Explanation of symbols]

 1,11 handle 2 support 3,13 blade 4 mounting part 5,8 protrusion 6 through hole 7 notch hole 9 groove

Claims (2)

(57) [Scope of request for utility model registration] 1. A scraper comprising a blade and a support thereof and a handle for holding the blade, the blade is made of a rubber-like elastic material, the support and the handle are made of plastic, and the scraper is The blade passing direction is perpendicular to the long axis direction of the handle, and the blade surface is inclined at an angle of 30 to 70 degrees with respect to the long axis direction of the handle, and is attached to the tip of the handle. A cell scraper characterized by being rotatable left and right within a plane perpendicular to the longitudinal direction of the handle in a range of 90 degrees, with the projection or through hole of the mounting portion as the axis. 2. A handle for holding a scraping portion, which is 3
The cell scraper according to claim 1, wherein the cell scraper is bent at an angle of 15 to 30 degrees at a position of 0 to 80 mm.