JP2531956Y2 - Small pieces for culture - Google Patents

Description

[Detailed description of the invention]

[0001]

BACKGROUND OF THE INVENTION The present invention is used in the fields of tissue culture, cell culture, etc., and is used for culturing cells or the like directly on the surface thereof, and performing staining and fixing as it is to obtain small pieces for culture which can be used as specimens. It is about.

[0002]

2. Description of the Related Art In the field of tissue culture, cell culture, and the like, in order to prepare a microscope specimen of cultured tissue or cells, the tissue or cells are generally cultured in a Petri dish or a plate having a plurality of wells. When doing, place the glass cover glass used when preparing the microscope preparation in a Petri dish or well, culture the tissue or cells on the cover glass in the medium, and after culturing, After removing the cover glass from the Petri dish or the well and fixing and staining the cells, the cover glass is adhered and fixed on a slide glass to prepare a specimen for a microscope. However, at this time, the cover glass used has a drawback that it is very easily broken and often breaks during handling.

[0003] In order to overcome the disadvantage that the cover glass made of glass is easily broken, the present inventors used a plastic film instead of glass and provided a claw portion formed by two cuts on the outer periphery. Invented and disclosed a small piece for culture which is easy to handle. (3)
No. 700)

[0004] In recent years, such small pieces for culturing made of plastic film have been generally used. However, since the specific gravity of plastic is lower than that of glass, the film floats in the medium during culturing. However, there has been a problem that cells and tissues adhere to and proliferate on both surfaces of the film.

[0005]

[Problems to be Solved by the Invention] The present invention is intended to solve such problems in the preparation of microscope specimens by culturing tissues and cells. It is an object of the present invention to provide a small piece for culturing, which can be easily adhered to the culturing surface of the above, and which is structurally simple and inexpensive.

[0006]

That is, the present invention has a thickness of 0.1 mm.
Small, semicircular, fan-shaped, elliptical or polygonal small pieces made of a plastic film of 0.5 to 0.30 mm. An adhesive was applied to one side of the small pieces and bonded to release paper. A culture piece.

Hereinafter, the present invention will be described in detail with reference to the drawings.

FIG. 1 and FIG. 2 are views showing examples of the shape of a small piece for culture according to the present invention. The small piece (1) is made of a plastic film, and is made of a material that has transparency for observation with a microscope, is hardly damaged,
There is no particular limitation as long as it has requirements such as chemical resistance and non-toxicity when culturing tissues or cells. Examples of such a plastic include polyester, polyethylene, polypropylene, and TPX resin. Further, one surface of the small piece (1) may be subjected to a hydrophilic treatment in order to improve the adhesion of the cells. As the method, plasma treatment, corona treatment, γ-ray treatment and the like are very generally used.

[0009] An adhesive is applied to the other side and bonded to release paper (4). When used, it is peeled off from the release paper and used, but it is desirable that the adhesive is transparent. Further, it is necessary that the material be water-resistant so as not to be peeled off for at least one week to 10 days at the time of culturing, and to be free from cytotoxicity due to eluted substances in the medium. , Urethane and acrylic adhesives can be used safely. On the other hand, the release paper is not particularly limited, and generally used paper such as silicone-coated paper can be used.

The adhesive may be applied to the entire surface of the small piece (1). However, in order to facilitate removal from the Petri dish or the bottom of the well after culturing, a part of the end of the small piece or shown in FIG. As described above, it is preferable to avoid applying the adhesive to the nail (3) formed by the two cuts (2) around the small piece (1).

The small piece coated with the adhesive is bonded to release paper to protect the coated surface and facilitate the handling, and is commercialized. However, the same shape as the small piece or FIGS. )
As shown in FIG. 3, small pieces (1) may be laminated one by one to a release paper piece large enough to accommodate one small piece to form a single piece product, or a sheet as shown in FIG. 3 (c). A plurality of small pieces (1) may be bonded to a release paper (4) in a shape of a cut sheet or a long rolled product. Such a product form may be appropriately selected according to the frequency of use of the small pieces for culture, convenience of use by the user, and the like.

The shape of the small piece (1) may be appropriately selected from a circle, an ellipse, a polygon and the like as shown in FIGS. 1 and 2, and the size and shape are made according to the wells of a Petri dish or plate to be used. I can do it. In addition, by devising the size and shape of the small piece (1) and making it semicircular or fan-shaped, a plurality of pieces can be used in one well. When the claw portion (3) is provided as shown in FIG. 2, the claw portion (3) can be bent as shown in FIG. 4 to provide a knob white when handling the small piece (1). ,preferable. In addition, the thickness of the small piece (1) is 0.05 to 0.30.
The thickness is preferably in the range of mm, but if it is thinner than 0.05 mm, it is difficult to maintain the shape, and if it is thicker than 0.3 mm, it will be an obstacle to microscopic observation.

When culture is started using the small pieces, first, the small pieces (1) are peeled off from the release paper (4), and the adhesive-coated surface of the small pieces (1) is brought into close contact with the Petri dish or the bottom of the well. At this time, a part of the end of the small piece (1) or the nail part (3) is bent, and is set as a grip portion by tweezers when the small piece (1) is taken out after the culture.

After the cell culture or tissue culture on the small piece is completed, the small piece (1) is taken out of the Petri dish or well, and attached to a slide glass (7) with an adhesive as shown in FIG. After fixing and staining the cells and tissues, the cells are covered with a cover glass (6) and stored as a specimen.

[0015]

[Effect of the Invention] The small pieces for culturing according to the present invention prevent breakage, which is a drawback of the conventional glass slide glass, and have no floating during culture, which is a drawback of the plastic cover slip. It can adhere to the bottom of the well and can be manufactured simply and at low cost.

[Brief description of the drawings]

FIG. 1 is a view showing an example of the shape of a culture piece according to the present invention.

FIG. 2 is a view showing another example of the shape of the culture piece according to the present invention.

FIG. 3 is a diagram showing a state in which the small pieces for culture according to the present invention are stuck to release paper.

FIG. 4 (a) is a perspective view of a culture piece provided with a nail portion,
(B) is a figure which shows the state which pinches a nail | claw part with tweezers and peels off release paper.

FIG. 5 is a diagram showing a structure of a specimen in which a small piece for culture is sandwiched between a slide glass and a cover glass.

Claims (2)

(57) [Scope of request for utility model registration] 1. A small piece of a plastic film having a thickness of 0.05 to 0.30 mm and having a circular, semicircular, fan-shaped, elliptical or polygonal shape, and an adhesive is applied to one surface of the small piece. And a small piece for culture characterized by being bonded to release paper. 2. A small piece of culture product, wherein a plurality of the small pieces of culture according to claim 1 are aligned and stuck on release paper to form a cut sheet or long roll.